BANDELIN SONOPULS HD 5020 User manual

Instructions for use
SONOPULS
Ultrasonic homogeniser
Valid for:
SONOPULS HD 5020
SONOPULS HD 5050
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© 2023
1 2 GmbH & Co. KG, Heinrichstraße 3 – 4, Germany, 12207 Berlin,
Phone: [phone] - 0, Fax: [phone], [email protected]
Certified in accordance with ISO 9001 and ISO 13485

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Table of contents
1 About this operating manual ........................................................................................................... 7
2 Safety .................................................................................................................................................. 8
2.1 Use of the device .............................................................................................................................8
2.2 Explosion hazard ..............................................................................................................................8
2.3 Hazards due to high temperature ..............................................................................................9
2.4 Danger of electric shock ................................................................................................................9
2.5 Danger due to ultrasound .......................................................................................................... 10
2.6 Danger due to the sonicating medium ................................................................................... 10
2.7 Harmful to health due to ultrasound noise .......................................................................... 10
2.8 Keep out of reach of children .................................................................................................... 10
2.9 Damage to the probe and sonication vessel ........................................................................ 11
2.10 Disturbance of wireless communication ............................................................................... 11
2.11 Safety labels on the device ........................................................................................................ 11
3 Design and function ........................................................................................................................12
3.1 Overview ......................................................................................................................................... 12
3.2 Symbols and buttons on the touch screen ........................................................................... 13
3.3 Function ........................................................................................................................................... 13
4 Preparation for operation ...............................................................................................................14
4.1 Set up homogeniser ..................................................................................................................... 14
4.2 Mounting/removing the probe ................................................................................................. 14
4.3 Switching the device on and off ............................................................................................... 16
4.4 Select probe .................................................................................................................................... 16

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5 Operation ..........................................................................................................................................17
5.1 Immersion depth notes .............................................................................................................. 17
5.2 Preselect parameters for sonication ...................................................................................... 17
5.2.1 Set amplitude or power .............................................................................................................. 17
5.2.2 Enter sonication time .................................................................................................................. 18
5.2.3 Set pulsation .................................................................................................................................. 18
5.2.4 Setting temperature monitoring .............................................................................................. 18
5.3 Start sonication ............................................................................................................................. 19
5.4 Creating and starting programs ............................................................................................... 20
5.4.1 About programs ............................................................................................................................ 20
5.4.2 Creating a program ....................................................................................................................... 20
5.4.3 Activating and deactivating a program .................................................................................. 20
5.4.4 Starting a program ....................................................................................................................... 22
5.5 Creating documentation ............................................................................................................. 23
5.6 Set settings .................................................................................................................................... 24
5.6.1 Adjusting the button function ................................................................................................... 24
5.6.2 Adjust brightness .......................................................................................................................... 24
5.6.3 Set the language ........................................................................................................................... 24
5.6.4 Setting time and date .................................................................................................................. 25
5.6.5 Set sleep mode .............................................................................................................................. 25
5.6.6 Configuring the network ............................................................................................................. 25
5.6.7 Set temperature unit ................................................................................................................... 26

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5.7 Troubleshooting a malfunction ................................................................................................. 27
6 Maintenance .....................................................................................................................................28
6.1 Cleaning the device ...................................................................................................................... 28
6.2 Refurbish probe ............................................................................................................................. 28
6.3 Repair ............................................................................................................................................... 29
7 Disposal .............................................................................................................................................30
8 Device information ..........................................................................................................................31
8.1 Technical data ................................................................................................................................ 31
8.2 Ambient conditions ...................................................................................................................... 32
8.3 CE conformity ................................................................................................................................ 33
9 Accessories .......................................................................................................................................34
9.1 Available probes ............................................................................................................................ 34
9.2 Optional accessories ..................................................................................................................... 35
9.3 Optional tool ................................................................................................................................... 36

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1 About this operating manual
These instructions for use contain necessary and useful information on how to operate the
device safely and efficiently.
• Read these instructions for use before using the device.
• Pay special attention to chapter 2 Safety.
• If you pass this device on to others, please enclose these instructions for use.
• Contact your specialist dealer or BANDELIN if any of your questions are not answered in
these instructions for use. Information on service can be found in chapter 6.3 Repair.
Illustrations are provided as examples and are not to scale.
About this operating manual

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2 Safety
2.1 Use of the device
The device is intended to be used in laboratories, clinics and in industrial research.
It performs diverse tasks during sample preparation in Quality Assurance, scientific
experiments, analyses, and in pilot or short-series manufacture. Only use the GM 5000
ultrasonic generators with the ultrasonic transducers from chapter 8 Device information.
Examples of applications are:
• Disruption of cells, bacteria, viruses, tissue
• Production of the finest emulsions
• Homogenising of substances
• Degassing of liquids
• Sample preparation for the grain size analysis
• Acceleration of chemical reactions
• Sample preparation for sewerage analysis
Do not sonicate non-liquid media. In case of improper use, the manufacturer does not
assume any responsibility for the safety as well as the functionality.
2.2 Explosion hazard
Do not use the device with combustible liquids as a sonicating medium.
Combustible vapours may be generated when flammable liquids are sonicated. Vapours may
ignite, e.g. on electrical components, resulting in an explosion.
Do not use combustible solvents in open sonication vessels, as the operational safety of the
device could be compromised. Safe extraction of combustible vapours must be guaranteed.
Safety

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2.3 Hazards due to high temperature
Ultrasound heats up the sonicating medium. The device, the sonicating medium and the
sonication vessel can become very hot during prolonged ultrasound operation. Contact with
these products may cause burns.
• Do not leave the ultrasound switched on for longer than necessary.
• Allow the probe and sonication vessel to cool down before touching them.
Non-aqueous liquids can heat up many times faster than water. A possible flashpoint can
be reached or exceeded after a very short sonication time. In the case of high-boiling liquids,
the temperature can rise to over 120 °C due to the energy input of the ultrasound. This can
lead to fires and severe burns.
2.4 Danger of electric shock
The device is an electrical device. Failure to follow safety rules can result in a life-threatening
electric shock.
• Protect the device from moisture and wetness. Keep the surfaces and the touchscreen
clean and dry.
• Do not spray or expose the device to splash water.
• Do not cover the device.
• Position the device so that it is easy to unplug the mains connection at any time and
without difficulty.
• The device must be plugged into a power outlet with an earthing socket that fits the
earthing contact on the device plug.

WARNING
For devices with type E+F plugs, please note:
Combining with type K sockets (particularly common in Denmark) is not permitted.
• Do not connect any external voltage sources to the external interfaces.
• If you notice a defect in the device, disconnect the mains plug immediately. Do not
connect a defective device to the mains.
• Repairs should only be carried out by the manufacturer.
Safety

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2.5 Danger due to ultrasound
Strong ultrasound destroys cell structures. Exposing a body part to ultrasound can lead
not just to skin damage, but also to internal tissue damage. On fingers, the periosteum can
become damaged.
• Do not touch the probe, the booster horn or the standard horn during operation. Do not
touch the sonication fluid during operation.
• Never sonicate living beings.
2.6 Danger due to the sonicating medium
The sonicating media treated with the device can be toxic, corrosive or otherwise harmful to
health. Vapours and aerosols can also be dangerous.
• Wear gloves and safety goggles when handling hazardous preparations.
• Make sure that the sonicating medium does not splash. This can particularly occur with
small quantities of sonicating medium. Only switch on the device once the probe has been
immersed in the sonicating medium. Start sonication with a low amplitude.
• Do not lean over the ultrasonic vessel, in order to avoid vapours from coming into contact
with the eyes or from being inhaled.
• In case of dangerous vapours, use a suction device.
2.7 Harmful to health due to ultrasound noise
The ultrasound noise typical of the process can be perceived as very unpleasant. Remaining
within a radius of 2 m for an extended period of time may cause damage to health.
• Use a sound proof box; see chapter 9 Accessories. The sound proof box significantly
reduces the noise level.
• When operating without a sound proof box, wear hearing protection.
2.8 Keep out of reach of children
Children cannot identify hazards posed by the device. Therefore, keep the device out of the
reach of children.
Safety

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2.9 Damage to the probe and sonication vessel
Probes are sensitive to shock. Handle the probes with care.
Make sure that the probe does not touch the sonication vessel. This can damage the probe
and the vessel.
Foreign bodies on the contact surface between the probe and standard horn or booster
horn can destroy both parts. Therefore, always clean the contact surfaces thoroughly before
mounting the probe.
The sound-emitting surface of the probe is subject to erosion. Erosion reduces the efficiency
of the sonication process. The sonication of corrosive liquids leads to increased wear.
The sound-emitting surface can be reconditioned up to a certain point of erosion. See
chapter 6.2 Refurbish probe.
2.10 Disturbance of wireless communication
The device may interfere with other wireless communication devices in the immediate
vicinity.
If interference occurs with the operation of a wireless device, move it further away from the
device.
2.11 Safety labels on the device
• Observe all safety labels on the device.
• Keep the safety labels in legible condition. Do not remove them. Replace them if they are
no longer legible. To do so, please contact our Customer Service. See chapter 6.3 Repair
Symbol Meaning Explanation
Wear ear pro-
tectors
Wear hearing protection or use a sound proof box when oper-
ating the unit.
Safety

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Design and function
3 Design and function
3.1 Overview
2
1
34 5 6 7
8
9
10
11
12
Overview of the device
1 Ultrasound generator
2 Touchscreen
3 Connector for ultrasonic converter
4 LAN connection
5 USB Type B port
6 USB Type A port
7 Mains switch
8 Cold device socket with fuse holder
9 Ultrasonic converter
10 Start/Stop key
11 Standard horn/booster horn
12 Probe

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Design and function
3.2 Symbols and buttons on the touch screen
Return to previous screen
Reset values
Confirm/Save
Go to startup screen
Calling up the screen help
Start
Pause
Stop
3.3 Function
The device convert the absorbed grid energy into high-frequency energy. The ultrasonic
converter converts the high-frequency energy into ultrasound. At the tip of the probe,
mechanical deflections are created that are transferred into the sonicating medium as
ultrasonic waves with a high power density. The standard horn or booster horn allows
multiple amplification of the mechanical amplitude at the probe tip.
For sonication, the power or amplitude can be selected as the reference variable. If the
amplitude is selected as the reference variable, it is kept constant by the ultrasonic converter
regardless of the load, provided that the permissible maximum power is not exceeded.
An optional temperature sensor enables the temperature of the sonicating medium to be
monitored.
The device saves documentation of its function. Documentation can be backed up via a USB
port or LAN port for evaluation and archiving.
The device is operated with a touchscreen. In addition, the ultrasound can be switched on
and off with a button on the ultrasonic converter.

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4 Preparation for operation
4.1 Set up homogeniser
1. Place the ultrasonic generator on a firm, level and dry surface.
• Make sure that the ventilation slots in the bottom plate of the ultrasonic generator
and the fan at the rear are not closed or blocked.
• Prevent liquid from entering the unit.
2. Screw the probe onto the ultrasonic converter; see 4.2 Mounting/removing the probe.
3. Clamp the ultrasonic converter on the black housing in a suitable holder. BANDELIN
recommends the HG 40 stand; see 9 Accessories.
4. If you are using a sound proof box, place the holder with the ultrasonic converter in the
sound proof box.
5. Insert the plug of the ultrasonic converter into the connection on the rear of the
ultrasonic generator.
6. Optional: Connect the temperature sensor to the ultrasonic converter.
7. Check that the mains switch is set to “0”. Insert the mains plug into a grounded socket.
4.2 Mounting/removing the probe
NOTICE
Damage to the ultrasonic converter
If the ultrasonic oscillating system in the housing of the ultrasonic converter twists, the
ultrasonic converter will be damaged.
- Always use the sickle spanner to secure the standard horn or booster horn.
- Never hold the ultrasonic converter by the housing when tightening or loosening the
probe.
Requirement
• The ultrasonic converter is disconnected from the ultrasonic generator.
• Tools required: 1 sickle spanner, 1 spanner (supplied tool set).
Preparation for operation

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Procedure
1. Clean the mounting surfaces of the ultrasonic converter and the probe.
2. Screw the probe on hand-tight.
3. Place the ultrasonic converter on a firm tabletop. In the case of probes, support the
probe tip with a thick underlay if necessary.
4. Place the sickle spanner on the ultrasonic converter. Support the sickle spanner on the
tabletop; see also the following figures.
5. Firmly tighten the probe with the spanner. The use of a torque wrench (Code No. 3662)
is recommended – not for HD 5020. The required tightening torque can be found in the
product information supplied with the probe.
A video on loosening and attaching the probe is available:
https://www.youtube.com/watch?v=hjTC_cvO4kQ
Tighten the probe
Loosen the probe
Preparation for operation

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4.3 Switching the device on and off
Switching on the ultrasonic homogeniser for the first time
Allow the device to stand for 2 hours at its operating location before switching it on so that it
can adapt to the climatic conditions.
1. Switch on the device using the mains switch on the rear of the ultrasonic generator.
» The “Language” screen appears.
2. Select your language.
» The “Time/Date” screen appears.
3. Tap the values to be changed. Enter the desired values. Confirm the entries with the
“Confirm/Save” button. Exit the “Time/Date” screen by pressing the “Confirm/Save”
button.
» The startup screen appears.
If the language and time are set, the startup screen will appear immediately the next time
the device is switched on. If the startup screen does not appear, see 5.7 Troubleshooting a
malfunction.
Switching the ultrasonic homogeniser off
Switch off the device using the mains switch on the rear of the ultrasonic generator.
4.4 Select probe
The control unit of the device automatically detects the connected ultrasonic converter. The
mounted probe must be selected via the touchscreen
1. On the startup screen, tap “Probe”.
2. Select the probe used.
3. Confirm your entry with the “Confirm/Save” button.
Preparation for operation

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5 Operation
5.1 Immersion depth notes
The recommended immersion depth for probes is 10 ... 20 mm, to prevent the aspiration and
mixing-in of air. If mixed-in air is desired, the probe may be immersed just a few millimetres.
When producing emulsions, the probe should be immersed to the height of the surface
separating the liquids to be mixed.
5.2 Preselect parameters for sonication
5.2.1 Set amplitude or power

Information
In the case of greater immersion depths or the sonication of liquids with high viscosities,
the probe is more heavily damped. As a result, the preset amplitude may not be reached.
The device can regulate the emitted ultrasonic power so that either a set amplitude is kept
constant or a set power is kept constant. The amplitude is given in %. The absolute value of
the amplitude depends on the combination of standard horn or booster horn and probe. The
power is specified in watts and depends on the respective ultrasonic converter.
1. On the startup screen, tap “Parameters”.
2. Tap “Ampl./Power”.
3. Tap “Amplitude” if sonication is to be carried out with a constant amplitude, or “Power”
if sonication is to be carried out with a constant power.
4. Select the desired value for amplitude or power. You can move the bar of the selected
setting parameter or enter a numerical value.
5. Confirm your entry with the “Confirm/Save” button.
With amplitude regulation, the amplitude is kept constant. The power can vary depending
on the condition of the medium. When the respective maximum power is reached, the
amplitude is reduced.
Power control keeps the power constant. The power can vary depending on the condition of
the medium. When the respective maximum amplitude is reached, the power is reduced.
Operation

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5.2.2 Enter sonication time
1. On the startup screen, tap “Parameters”.
2. Tap on “Time”.
3. Make the desired settings for the sonication time.
» A time setting is only effective if “Continuous operation” is not activated.
4. Confirm your entry with the “Confirm/Save” button.
5.2.3 Set pulsation
The sonication can be switched on continuously or in pulses.
1. On the startup screen, tap “Parameters”.
2. Tap “Pulsation”.
3. Turn pulsation on or off.
4. Enter how long you want the sonication to be alternately switched on and off.
5. Confirm your entry with the “Confirm/Save” button.
5.2.4 Setting temperature monitoring
With the BANDELIN temperature sensor TM 5000 you can monitor the temperature of
the sample during sonication. You can set whether the ultrasound is switched off or only a
warning tone is emitted when a limit value between 0 °C and 100 °C is reached. To do this,
connect the TM 5000 temperature sensor to the lid of the respective ultrasonic converter
after removing the dust cap. The temperature now appears on the display.
1. On the startup screen, tap “Parameters”.
2. Tap “Temperature.”
3. Make the desired changes. To set the temperature, you can move the bar or enter a
numerical value.
4. Confirm your entry with the “Confirm/Save” button.

Information
Only the TM 5000 temperature sensor is compatible with the unit.
Operation

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Operation
5.3 Start sonication
Requirement
• The probe tip is immersed in the sonicating medium; see 5.1 Immersion depth notes.
1. On the startup screen, tap the “Start” button.
» The “Sonication” screen appears.
» The preselected sonication parameters are displayed; see chapter 5.2 Preselect
parameters for sonication.
2. Optional: Tap one of the buttons “Amplitude,” “Power,” “Time,” “Pulsation” or
“Temperature” to change the preselected settings for the current sonication. Confirm
the new settings by tapping the “Confirm/Save” button.
3. Tap the “Start” button at the bottom of the screen.
» The sonication begins.
» If the timer was set, the sonication ends automatically after the set time.
» You can interrupt the sonication at any time with the “Pause” button or end it with
the “Stop” button.
» After the end of sonication, a screen with a summary of the sonication appears.
4. Tap the “Confirm/Save” button
» The sonication screen reappears with the last settings.

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5.4 Creating and starting programs
5.4.1 About programs
Programs are saved parameter sets for sonication. Amplitude or power, time and pulsation
are stored in a program. A total of eight programs can be stored. Each program can be active
or inactive. The sequence in which the programs are executed can be defined as required.
5.4.2 Creating a program
1. On the startup screen, tap “Programs”.
» The “Programs” overview screen is shown.
2. Tap on a program number.
3. If a screen with the summary of the program parameters appears, tap “Edit”.
4. Tap the “Ampl./Power,” “Time” or “Pulsation” button to edit the program parameters;
see 5.2 Preselect parameters for sonication. Confirm your entry with the “Confirm/
Save” button.
5. Tap the “Confirm/Save” button to save the program.
5.4.3 Activating and deactivating a program
Activating a program
1. On the startup screen, tap “Programs”.
» The “Programs” overview screen is shown.
2. Tap on a program number.
3. If a screen with the summary of the program parameters appears, tap “Edit”.
4. Under “Sequence,” select the position in the sequence of programs.
5. Set the sliding switch to “on”.
6. Tap the “Confirm/Save” button to save the activation.
7. Tap the “Back” button on the screen with the summary of the program parameters.
» The “Programs” overview screen is shown.
» Activated programs are shown in colour.
» For programs that are assigned a position in the sequence, the three dots under the
program number are shown in black.
Operation