Qiagen EZ1 Advanced XL User manual

Sample to Insight__
November 2021
Quick-Start Protocol
EZ1&2™ ccfDNA Kit
The EZ1&2 ccfDNA Kit (cat. no. 954854) is shipped at room temperature (15–25°C). Upon
receipt, store all kit components dry at room temperature.
Further information
 EZ1&2 ccfDNA Kit Handbook : www.qiagen.com/HB-2915
Safety Data Sheets: www.qiagen.com/safety
Technical assistance: www.support.qiagen.com
Notes before starting
Plasma samples should be at room temperature before use. If plasma/serum was stored
frozen, thaw the samples by incubating at 37°C in a water bath.
If the sample volume is less than 2 ml, 4 ml, or 8 ml, adjust the volume with PBS to 2 ml,
4 ml, or 8 ml.
Procedure
1. Load reagent cartridges into the cartridge rack.
2. Resuspend Magnetic Bead Suspension EZ by vortexing briefly and then tap to ensure all
the liquid is at the bottom of the tube. Remove the cap from the tube with the bead
solution placed in position 11 of the EZ1&2 ccfDNA cartridge (see Figure 1).

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EZ1&2 ccfDNA Kit 11/2021
Figure 1. EZ1&2 ccfDNA cartridge.
3. Transfer 900 µl Elution Buffer EZE into position 12 of the EZ1&2 ccfDNA cartridge.
4. Remove caps of all tubes and prepare the Tip Rack as follows (see Figure 2):
Position 4/A: Tip holder with Filter Tip (provided)
Position 3/B: new large volume tube (7 ml) (provided)
Position 2/C: new large volume tube (7 ml) (provided)
Position 1/D: new 1.5 ml elution tube (provided)
Figure 2. Tip Rack - Large Volume.

EZ1&2 ccfDNA Kit 11/2021
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5. 2 ml/4 ml protocol: Transfer 2 ml/4 ml sample into the 7 ml tube in position 2/C. Leave
the 7 ml tube in position 3/B empty.
8 ml protocol:Split your sample and transfer 4 ml into each of the 7 ml tubes in positions
2/C and 3/B.
Procedure on the EZ2®Connect
1. Turn on the EZ2 Connect instrument.
2. Tap “DNA” on the Applications panel and select the “EZ1&2 ccfDNA Kit” and press
Next. Follow onscreen instructions for selection of protocol, parameter definition, sample
position selection, sample IDs, and worktable setup.
3. Open the instrument door. Load the cartridge rack into the instrument.
4. Place the tip rack into the instrument.
5. Close the instrument door; press START to initiate the EZ1&2 ccfDNA protocol.
6. The display will show “Protocol finished” when the run is completed. Select Finish.
Open the instrument hood. Remove the elution tubes containing the purified ccfDNA (in
75 µl) from position 1/D of the tip rack. Discard the sample preparation waste.
Optional: Follow onscreen instructions for UV decontamination of worktable surfaces.
7. Perform regular maintenance after each run. Press Finish to return to the Home Screen.
Procedure on the EZ1®Advanced XL
1. For use on EZ1 Advanced XL instrument, insert the EZ1 Advanced XL ccfDNA Card
completely into the card slot and turn on the instrument. Please make sure the instrument
is turned off prior to inserting the card.
2. Press START to initiate the EZ1&2 ccfDNA protocol. Follow onscreen instructions for
selection of protocol, data tracking, and worktable setup. Close the instrument door;
press START to start the protocol.
3. Open instrument door. Load the cartridge rack into the instrument.
4. Place the tip rack into the instrument.

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EZ1&2 ccfDNA Kit 11/2021
5. The display will show “Protocol finished” when finished. Press ESC.
6. Open the instrument door. Remove the elution tubes containing the purified ccfDNA (in
75 µl) from position 1 of the tip rack. Discard the sample preparation waste. Press ENT.
The report file is transferred automatically.
Optional: Follow onscreen instructions for UV decontamination of worktable surfaces.
7. Perform regular maintenance after each run. Press ESC to return to the Main Menu.
Document Revision History
Date Changes
11/2021 Initial release.
Scan QR code for handbook.
For up
-to-date licensing information and product-
specific disclaimers, see the respective
QIAGEN kit handbook or user manual.
Trademarks: QIAGEN
®, Sample to Insight®, EZ1®, EZ1&2™, EZ2®(QIAGEN Group). Registered names, trademar
ks, etc. used in this document, even when not
specifically marked as such, are not to be considered unprotected by law.
1126415 11/2021 HB-2891-002 © 2021 QIAGEN, all rights reserved.
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