BestScope BS-2073 Series User manual

Biological Microscope
BS-2073, BS-2074 Series
Instruction Manual
BS-2073B BS-2074B
This manual is for the biological microscope Model BS-2073, BS-2074. To ensure the safety,
obtain optimum performance and to familiarize yourself fully with the use of this microscope, it is
recommended strongly that you study this manual thoroughly before operating the microscope.

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CONTENTS BS-2073,BS-2074
User Notices ............................................................................................2
1. Components Names.............................................................................4
2. Assembly..............................................................................................5
2.1Assembly Diagram................................................................................................................5
2.2Assembly Procedure.............................................................................................................6
3. Adjustment And Operation ..................................................................9
3.1Adjustment Set Diagram ......................................................................................................9
3.2Operation...........................................................................................................................13
4. Video Attachment..............................................................................18
4.1Installing Trinocular Viewing Head And Video Attachment ...............................................18
4.2Focusing the Specimen ......................................................................................................18
5. Digital Viewing Head..........................................................................19
5.1Digital Viewing Head..........................................................................................................19
5.2 Installing............................................................................................................................19
6. Fluorescent Observation ....................................................................20
6.1Fluorescent Set...................................................................................................................20
6.2Installing.............................................................................................................................20
6.3Method of Application.......................................................................................................20
7. Phase Contrast Observation...............................................................21
7.1Components Name ............................................................................................................21
7.2Installation And Use ...........................................................................................................21
8. Dark Field Observation.......................................................................23
8.1Bright and Dark Filed Components ....................................................................................23
8.2Installation And Use ...........................................................................................................23
9. Simple Polarization Set.......................................................................24
9.1Components Name ............................................................................................................24
9.2Installation And Use ...........................................................................................................24
10. Technical Specifications....................................................................25
11. Trouble Shooting..............................................................................26

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Use Notices BS-2073,BS-2074
Ⅰ. Configuration Instruction
1. BS-2073 is the basic model and uses NIS45 system;
2. BS-2074 is coding model, using NIS60 system, with brightness memory, wire resistance stage and other
configurations.
Ⅱ. Principle and Application
Principle: Using the appropriate adjustment combination of two lens sets, the specimen is magnified and
observed. The inverted real image is obtained through objective, then the image is further
magnified by the eyepiece.
Application: Biological microscope is used by medical and health units, colleges and universities, research
institutes for the observation of microorganism, cell, bacteria, tissue culture, suspension,
sediment,etc.
Ⅲ. Safety Marks
The Following marks are on the microscope. Figure out the meaning of these symbols and always use the
microscope in the safest way.
Ⅳ. Safety Note
1. Open the box carefully to avoid the accessories, like lens, being polluted by fingerprint or sweat strain,
dropping to ground and being damaged.
2. Do keep the microscope out of direct sunlight, high temperature or humidity, dusty and easy shaking
environment. Make sure the stage is flat, horizontal and firm enough.
3. When moving the microscope, carefully carry it with the handle and the base.
4. If the bacterium solution or the water splash to the stage, objective or viewing tube, pull out the power
cord at once, and wipe up the microscope. Otherwise, the instrument will be damaged.
5. To keep at least 10cm distance all around between the microscope and other objects for ventilating and
cooling.
6. Make sure the instrument is earthed, to avoid lighting strike.
7. For safety, be sure the main switch is in “O” (off) state and cut off the power supply before replacing
the fuse. Check the input voltage: be sure the input voltage which is signed in the back of the microscope
is consistent with the power supply voltage, or it will bring a serious damage to the instrument.
8. Always use the power cord provided by us.
Symbol
Meaning
Power on
Power off
/
Up Lighting/Down Lighting
F.S.: Field Stop, :Expanding F.S. :Narrowing F.S.
Direction of light intensity. When turning to the tip, the intensity
decreases from strong to weak
SLEEP
Dormant State
LOCK
Light intensity is locked

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Ⅴ. Maintenance and Care
1. All the lenses have been adjusted properly, do not dismount them by yourself please.
2. The nosepiece and coarse and fine focusing parts are so delicate that it is forbidden to disassemble them
carelessly by yourself.
3. Keep the instrument clean, wipe dust every day, and do not pollute the optical element when wiping
away the dust on the instrument. Clean the Objective by professional staff once a month.
4. The contaminations on the prism, as finger mark and oil, could be gently wiped with a piece of lens
tissue which has been immersed in a small amount of the mixture of xylene(70%) and alcohol(30%). Note
that the xylene and the alcohol are all burned easily. When in use, do not operate the power switch of
various electrical equipment, and do not let them near the fire. Please make sure the room is ventilation.
5. Don’t use organic solvent to wipe the non-optical elements, when you need to clean, use the soft
detergent, please.
6. When using, if the microscope is splash by liquid, cut off the power at once, and wipe up the moisture.
7. Do not disassemble any parts of the microscope. That will affect the function or decline the
performance of the microscope.
8. Place the instrument in a cool, dry position. After using the microscope, remember to cover it with dust
helmet. Do wait for the lamp house cooling completely before cover.
9. Using environment:
a) Use indoor;
b) Working temperature: 0℃~40℃;
c) Maximum relative humidity: it is 80% for temperature of 31℃, the following is the linear decrease,
70% for 34℃, 60% for 37℃, 50% for 40℃;
d) The highest elevation is 2000m。
10. Storage and transport environment:
a) Range of working temperature: -25℃~+65℃
b) Range of relative humidity: 0%~90%
Warnings:
It may harm the safety of the users if not operating in accordance with the instructions. In addition, it
may damage the microscope. Please operate the microscope strictly according to this instruction.
Transmitted light sources (condenser or collector) may produce harmful optical radiation. Do not look
directly at the transmitted light, which may cause eye damage
◎This microscope will not cause radiation and the electromagnetic interference to the surrounding
environment, totally accordance with the EMC certification standards.
This instruction uses these symbols to mark prominent words:
means it will hurt the operator or damage the equipment (objects around included)
without paying attention to the warnings in this instruction.
★means it will damage the equipment if not following the instruction.
◎means comments (for operating and maintenance)

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1.Components Name BS-2073,BS-2074
Model BS-2074
Model BS-2073
Nosepiece
Condenser
Eyepiece
Stage
Viewing head
Objective
Main body
Condenser
Eyepiece
Viewing head
Encoded nosepiece
Stage
Main body
Objective

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2.Assembly BS-2073,BS-2074
2.1 Assembly Diagram
The diagram below shows how to assemble the various components. The numbers indicate the order of assembly.
When assembling the components, make sure that all parts are free of dust and dirt, and avoid scratching
any parts or touching glass surfaces.
Keeping the hexagon- spanner well, when change the spare parts, you will use it.
①
②
Objective
Power cord

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2.2 Assembly Procedure
2.2.1 Installing the Objective (Fig.1-2)
1. Adjust the coarse focus knob until the support device of
the mechanical stage reach its low limit position.
2. Screw the lowest magnification objective into the
nosepiece from the left or the right side, then revolve the
nosepiece clockwise/counterclockwise and mount other
objectives by the sequence of low to high magnification.
◎Installing objective this way will make the change of
magnification to be easier during using.
★BS-2074 Model: Turn on the power supply. The
corresponding objective magnification on the LCD screen
will be highlighted (as shown in figure 3) when the
nosepiece moves to a certain position. Then install the
corresponding objective at this location.
★Clean the objective regularly, for lens is susceptible to
dust.
★When operating, use 10× magnification objective to
search and focus specimen firstly, then replace with
higher magnification objective if necessary.
★When replacing the objective, slowly turn the nosepiece
until you hear “clicked”, that means the objective enter
the required position--the light path center.
Fig.2
Fig.1
Fig.3
10X

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2.2.2 Connecting the power cord (Fig.4-5)
★The cable and cords are vulnerable when bent or twisted,
never subject the power cord to excessive force.
1. Set the main switch① to “O” (off) state before connecting
the power cord.
2. Insert the plug② into the power socket ③of the
microscope safely. Make sure the connection is well.
3. Insert the plug④on the other end of the power cord into
the power supply receptacle⑤ safely. Make sure the
connection is well.
★Do use the supplied power cord all the time. If lost or
damaged, select the same standard cord, please.
★Insert the power adapter into the holeⅡto charge for
charging model.
★Wrap the power cord around the back cover I when not in
use.
★Make sure that used voltage is consistent with the
required input voltage of instrument. If it is inconsistent,
please contact the supplier.
2.2.3 Replace the fuse (Fig.6-7)
The main switch must be set to the state “O” (OFF) before
replacing the fuse. Open the drawer of the fuse box by
Allen wrench at first. Then as shown in the figure 7, push
the fuse out gently through square hole under the fuse
drawer with Allen wrench.
★The middle of the fuse is thin glass. Please be careful
when you open the fuse drawer and push out the fuse.
Fig.4
Fig.5
Fig.6
①
②
③
I
Ⅱ
Fig.7

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2.2.4 Mounting the filters (Fig.8)
Place the required filter into the appropriate hole of the
collector.
2.2.5 LED lamp replacement (Fig.9-11)
◎Generally, LED lamp is very durable, so it is not easy to
damage. If it is unfortunately damaged, please purchase the
LED (Fig.11) from your vendors.
1. Loosen the screw “I”of brightness adjustment knob to take
the brightness adjustment knob down. Loosen six screws of
the bottom plate to open the bottom plate①;
2. Loosen screw② to take the LED lamp③ down and unplug
the connector④from the breadboard. Mount new LED
lamp, tighten screw② and insert the connector fully into
the breadboard.
★Note: Be careful to take the bottom plate down slowly in
case that the wire inside the microscope is pulled apart.
Fig.9
Fig.10
Fig.11
④
①
②
③
I
Fig.8

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3.Adjustment And Operation BS-2073,BS-2074
3.1 Adjustment Set Diagram
Model BS-2073
Tension Adjustment
Collar
Interpupillary Distance
Indicator
Aperture Diaphragm
Main
Switch
Brightness Adjustment
Knob
Left Coarse
Focus Knob
Left Fine Focus Knob
Collector
Phase Contrast Slider
Condenser Focusing Knob
Field Stop

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BS-2073,BS-2074
Portrait Adjustment Knob
Lateral Adjustment Knob
Power Socket
Right Fine Focus Knob
Locking Knob

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BS-2073,BS-2074
Model BS-2074
LCD Screen
The symbol is converted
to” ”when up lighting
Time adjustable, time division 1min
The number changes from 0 to
100, the change value is 5 for one
grid
Solid blue dot
Number adjustable
LCD(Liquid Crystal Display)
Phase Contrast Slider
Main
Switch
Brightness Adjustment
Knob
Left Coarse
Focus Knob
Tension Adjustment Collar
Condenser Focusing Knob
Aperture Diaphragm
Interpupillary Distance
Indicator
Left Fine Focus Knob
Collector
Filed Stop

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BS-2073,BS-2074
Portrait Adjustment Knob
Lateral Adjustment Knob
Power Socket
Right Fine Focus Knob
Locking Knob
USB Interface

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3.2 Operation
Fig.12
①
②
3.2.1 Brightness Adjustment (Fig.12-15)
1. Connect the power cord and set the main switch① on the
right side of microscope to “－”state (ON).
2. Turning the brightness adjustment knob② in the direction
shown in the figure, the voltage raise, and the brightness
strengthen; whereas turning at the contrast direction, the
voltage decline, and the brightness weaken.
◎Other operation of brightness adjustment knob for
BS-2074 model
1. Click Knob: enter the standby state and “SLEEP” appears on
the screen,as shown in figure 13. Click again to
eliminate the state, the “SLEEP” on the screen disappears
and the normal working state is displayed;
2. Long press the knob for 3s: choose to set sleep after the
fixed time, and the value of minute starts to beat. The
value of hours tarts to beat after clicking the knob. The
time can be increased or reduced by turning the knob. The
increase or decrease of lattice value is 1 minute and the
maximum value can be set to 8 hours. After setting to the
required time, the time number beats three times and then
stops beating, which meaning the setting is successful.
Time begins to decrease by minute;
Fig.13
Fig.14
TIME
SLEEP

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Fig.15
3. Double-click Knob: lock brightness or unlock (Fig.15). The
brightness adjustment knob fails when locking, and ”LOCK”
appears on the LCD screen; Double-click the knob again to
release the lock and the “LOCK” on the screen disappears;
1. Press+upward rotation: switch to up lighting;
5. Press+downward rotation: switch to down lighting.
★”LOCK” means that the user sets specific brightness when
using a certain magnification objective, and uses the lock
function to prevent it from being changed by another user.
(In this case, when switching to another magnification
objective, the brightness automatically change to the
brightness of the corresponding magnification, but the
brightness adjustment knob fails.

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3.2.2 Placing the Specimen (Fig.16-17)
1. Place the specimen in the center of the mechanical stage
and use the stage clips to clamp it.
2. Turn the portrait and lateral adjustment knob①of the
mechanical ruler, move the specimen to the required
position.
★Be careful when changing the objective. If you finish the
observation with the short working distance objective,
and want to change another one, be careful of not letting
the objective touch the specimen.
3.2.3 Adjusting the Interpupillary Distance (Fig.18)
The interpupillary distance range:47mm～78mm. While
looking through the eyepieces, move both eyepieces until
the left and right fields of view coincide completely.
3.2.4 Focusing the Specimen (Fig.19)
1. Focus the specimen with 10X objective. To avoid the
objective touching the specimen during focusing, you
should raise the mechanical stage to let the specimen
close to the objective at first, then slowly separate them
to bring the specimen to focus.
2. Turn the coarse focus knob conversely to lower the
specimen and search images in the 10×ocular
simultaneously, and then use the fine knob② to focus.
After that, you can replace with other magnification
objectives safely, and focus without the risk of damaging
the specimen.
★To make the observation more convenient,
you can use the locking set③ to fix the
stage in a vertical direction.
Fig.16
Fig.17
Fig.18
Fig.19
②
③
①

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3.2.5 Adjusting the tension adjustment collar (Fig.20)
★The tension of the coarse focus knob had already
adjusted before leaving factory. If it is too loose(the
mechanical stage slides down because of its own
weight), please turn the tension adjustment collar①
until the tightness is appropriate. When the collar is
turned in the direction of the arrow, tension of the
coarse adjustment knob increases. Turning the collar in
the opposite direction decreases the tension.
If the stage descends on its own or if the specimen gets
out of focus quickly even when it is brought into focus
using the fine adjustment knob, it means the tension of
the coarse adjustment knob is too low. Turn the collar in
the direction of the arrow to increase the tension
3.2.6 Aperture Iris Diaphragm Adjustment (Fig.21)
Turn the aperture iris diaphragm collar②to adjust the
aperture iris diaphragm. Turn the scale mark of aperture
iris diaphragm collar to the same number as the
objective magnification.
★The aperture iris diaphragm is designed for the
adjustment of the numerical aperture, not for the
brightness. Generally, setting the aperture iris
diaphragm to 70- 80% of the N.A. of the objective in
use will provide an image with good contrast. If you
want to observe the image of the aperture iris
diaphragm, remove one eyepiece and look through the
tube. You will see a dark circle encroaching on the
bottom of the tube.
Fig.20
Fig.21
②
①

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3.2.7 Adjustment of Field Stop (Fig.22)
Turn the field stop ring③in the direction shown in
the figure to close the filed stop. Turn the field stop
ring in the opposite direction to increase the filed stop.
According to the magnification of the objective, adjust
the filed stop until filed stop is circumscribed with
filed which will reduce the mixed light and improve the
quality of the image.
3.2.8 Condenser Adjustment (Fig.23-24)
Turn the condenser focus knob① to move the
condenser up and down. Raise the condenser when
using the high magnification objective, and descend it
when using the low magnification one.
Centering the Condenser:
1. Adjust the condenser focus knob① to the highest
position.
2. Focus the specimen with 10× objective.
3. Rotate field stop ring②until the image of field stop
can be observed.
4. Adjust the condenser focusing knob① to focus the
image of field stop.
5. Rotate the centering screw③by Allen wrench to
center the field stop.
6. Gradually open the field stop until the image of the
field stop inscribed with the field of viewing. That
means the condenser is centred correctly.
7. Slightly increase the field stop in reality operation to
make the image circumscribed with the field.
Fig.23
Fig.24
③
④
①
③
Fig.22
②

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4.Video Attachment BS-2073,BS-2074
4.1 Installing Trinocular Viewing Head And Video
Attachment (Fig.25)
1. Mount the trinocular viewing head according to the
assembly path①in Fig.25 into the round dovetail of
the microscope and tighten the screwⅠto fix the
viewing head;
2. Mount the video attachment according to the assembly
path② into the connecting seat of the trinocular
viewing head and tighten the screw Ⅱ;
3. Mount the thread interface of CCD or CMOS according
to the path③ into the video attachment.
4.2 Focusing the Specimen
After obtaining clear image by binocular observation,
observe the image on the computer or the monitor.
If it is not clear, please turn the focusing ring Ⅲon the
video attachment until the image is sharp enough.
◎The CCD/CMOS will not rotate following the rotation of
focusing ring Ⅲto avoid the entanglement of data
line, which is more convenient.
◎Binocular/Trinocular Switching Viewing Head (Fig.26)
Select the required optical path with optical path
switching knob④
Sign
Eye: Camera (%)
100:0
0:100
Fig.25
Fig.26
Ⅰ
①
②
Ⅱ(back)
③
Ⅲ
④

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5.Digital Viewing Head BS-2073,BS-2074
Fig.28
5.1 Digital Viewing Head (Fig.27-28)
There are two types of digital viewing heads to choose
from: wired digital viewing head (Fig.27) and wireless
digital viewing head(Fig.28).
1. The wired digital viewing head only has
one interface. It is connected to the computer through
the data line. The software ScopeImage 9.0 should be
installed on the computer to process image;
2. On the wireless digital viewing head, ①
is connected to the mouse; ②is the power socket,
connected to the power supply; ③is the HDMI
interface, the other end can be connected to the
computer or mobile phone with Touchscope Pro
software; ④is connected to the network cable.
5.2 Installing (Fig.29)
Mount the digital viewing head according to the
assembly path in Fig.29 into the round dovetail of the
microscope and tighten the screw⑤to fix the viewing
head.
Fig.29
Fig.27
①
②
③
④
⑤