BestScope BS-6000B User manual

Inverted Metallurgical
Microscope
Model Number
BS-6000B
User Manual
This manual is written for Inverted Metallurgical Microscope BS-6000B. Toensurethesafety,obtain optimum
performance and to familiarize yourself fully with the microscope, it is strongly recommended that you readthis
manual carefully before operating the microscope.

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Contents
User Notice.............................................................................................................................................2
I. Safety Precautions...............................................................................................................................2
II. Maintenance and Care....................................................................................................................... 2
III. Safety Symbols.................................................................................................................................3
1. Name of Components.........................................................................................................................4
2. Assembly Procedures......................................................................................................................... 5
3. Adjustment Set Diagram.................................................................................................................. 10
4. Adjustment Procedures.....................................................................................................................12
4-1 Microscope Base............................................................................................................................ 12
4-2 Mechanical Stage........................................................................................................................... 14
4-3 The Viewing Tube.......................................................................................................................... 15
5. Microscope Video and Photography................................................................................................ 16
5-1 Microscope Video.......................................................................................................................... 16
5-2 Microscope Photography............................................................................................................... 17
6．Technical Specification.................................................................................................................. 19
6-1 Main Technical Specification.........................................................................................................19
6-2 Objectives Specification................................................................................................................ 19
7．Trouble Shooting............................................................................................................................ 20

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User Notice
I. Safety Precautions
1. Be careful when moving the microscope, to avoid the lenses dropped or damaged.
2. Do keep the microscope out of direct sunlight, high temperature or humidity, dusty and easy shaking
environment. Make sure the stage is smooth, horizontal and firm enough.
3. When moving the instrument, grip two sides of the bottom of the microscope with your two hands.
4. When running, the lamp house and nearby parts will be very hot. Please ensure there is enough cooling
room for them.
5. Make sure the instrument is earthed, to avoid lighting strike.
6. For safety, be sure the main switch is in “O”(off) state and cut off the power supply before replacing the
bulb or the fuse. If you replace the bulb during use or right after use, allow the lamp bulb and the lamp
house to cool completely before touching.
(Designated bulb: 6V30W Halogen Lamp)
7. Check the input voltage: be sure the input voltage which is signed in the back of the microscope is
consistent with the power supply voltage, or it will bring a serious damage to the instrument.
II. Maintenance and Care
1. All the lenses have been adjusted properly; do not dismount them by yourself please.
2. The nosepiece and coarse and fine focusing parts are so delicate that it is forbidden to disassemble them
carelessly by yourself.
3. Keep the instrument clean ,and do not pollute the optical element when wiping away the dust on the
instrument.
4. The contamination on the prism, like fingerprints and oil smudges, could be gently wiped with a piece
of soft cloth or tissue paper, gauze which has been immersed in pure alcohol or ether. (Note that the
alcohol and ether are highly flammable, do keep them away from the fire or potential sources of
electrical sparks, and use them in a drafty room as possible as you can.)
5. Do not attempt to use organic solvents to clean the microscope components other than the glass
components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral
detergent.
6. When using, if the microscope is splashed by liquid, cut off the power at once, and wipe away the
splash.
7. Do not disassemble any parts of the microscope, as this will affect the function or reduce the
！Make sure that the input voltage is consistent with the power
supply voltage, or it will bring a serious damage to the instrument.

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performance of the microscope.
8. Place the instrument in a cool, dry position. After using the microscope, remember to cover it with dust
helmet. Do wait for the lamp house cooling completely before cover.
III. Safety Symbols
Symbol Explanation
Indicates that the surface becomes hot, and should not be touched with bare hands.
Before use, carefully read the user manual .Improper use could result in personal injury to
the user and/or damage to the equipment.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.

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1. Name of Components
Fig.1
Trinocular
Viewing Head
Microscope Body
Stage Stage center plate
NosepieceObjectiveMetallurgical
Illumination System
eyepiece

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2. Assembly Procedures
Fig.3
Fig.2
2-1 Mounting the Objectives
(Fig.2 and Fig.3)
1. Turning the coarse focusing knob①as the figure shows
till the nosepiece gets to its lower limit.
★For ensuring the safety of the instrument during
transportation, the nosepiece is located in the lowest
position and the tension adjustment collar②is adjusted
to an appropriate tension while leaving the factory.
2. Screw the lowest magnification objective on to the
nosepiece from the nearside, then turn the
nosepiece clockwise, mount other objectives according the
magnification sequence of low to high.
◎Mounting objective in this way will make it easier to
change magnification.
◎The objective can also be mounted through the opening
on the stage.
★Clean the objective periodically, for the objectives used in
the inverted microscope are susceptible to dust.
★Do cover all the unused holes③on the nosepiece with
dust caps, to prevent the dirt and dust from getting inside.
★When operating, use the low magnification objective
(4X or 10X) to search and focus the specimen at first, then
replace with the higher magnification objective if
necessary.
★When replacing the objective, turn the nosepiece slowly
until you hear “clicked”, that means the objective enters
into the right position, center of the light path.
①②
③

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Fig.5
Fig.6
2-2 Mounting Auxiliary Stage and the
Mechanical Ruler
◎Auxiliary stage can be installed in either side of the
stage to enlarge the stage surface. But you can’t install
the mechanical ruler at the same side.
◎Generally, the mechanical ruler will be installed at
the right side for comfortable operation.
1. Installing the auxiliary stage
First, Screw the clamping bolts into to the auxiliary
stage, then into the main mechanical stage from below
on right or left side; Tighten it with a screwdriver until
the auxiliary stage is securely attached.
2. Installing the mechanical ruler
Please install the ruler in the same way as the
auxiliary stage.
2-3 Installing and Replacing the Bulb
(Fig.4-Fig.7)
Please use the specified halogen Lamp 6V30W.
1. Pull out the lamp house①asshowninfig.4
2. Holding the bulb ①with a piece of gauze or other
protection materials, insert the bulb pins straight and
fullyintothepinholes③on the lamp house. (Fig.5)
3. Bulb replacement during use or right after use. The
bulb, the lamp house and nearby parts will be very hot.
Please set the main switch to “O” (off), disconnect the
power cord, and make sure the bulb, the lamp house
and periphery are all cool. Then, you can do your
replacing.
Fig.4

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Fig.7
Fig.8
Fig.9
Please insert the lamp gently, or it will be damaged
by excessive extrusion.
Do not touch the halogen bulb with bare hands. It
will shorten the service life or cause it to burst. If you
leave fingerprints on the surface carelessly, clean it
withapieceofdrysoftcloth.
4. Align the socket①with pins③,and the bolts②with the
jacks④.then push the lamp-house all the way into
place.(Fig.6-7)
2-4 Installing the Stage Center Plate
（Fig.8）
1. When using the glass stage ①, there is no special
requirement, you just need to place it in the center of the
mechanical plain stage.
2. Install the stage center plate on to the stage opening.
2-5 Installing the Eyepiece (Fig.9)
1. Remove the cap of the eyepiece tube.
2. Insert the eyepiece into eyepiece tube until they are
against each other.
2-6 Mounting the video (photography)
adapter(Fig.10,11,12)
Insert the video (photography) adapter tube (Fig.10)
into the trinocular viewing head (Fig.11), and screw down
the bolt to fix it, as shown in Fig.12.
Fig.10 Fig.11
①

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Fig.13
Fig.12
Fig.14
①
②
①
2-7 Mounting Filters and Polarizer
(Fig.13)
Insert the filter①into the corresponding jack.
For polarization observation, insert the polarizer②
into the right jack, as shown in Fig.13.
★Standard filters include blue, yellow and green
filters.
2-8 Mounting Analyzer (Fig.14)
Insert the analyzer①into the jack under the
trinocular viewing head if necessary, as shown in
Fig.14
★Analyzer can be used alone or matched with the
polarizer.

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④
Fig 15
Fig.16
2-10 Replacing the Fuse (Fig.15-16)
Do remember to set the main switch①to the
state of “O” (OFF) and unplug the power cord
before replacing the fuse. Rotate the fuse⑦kits out
of the holder⑧with the “--”type screwdriver,
replacewithanewfuse,thenrotateitbacktothe
holder again.
★Fuse rating: 250V, 500mA.
2-9 Connecting the Power Cord
(Fig.15, Fig.16 and Fig.17)
★The cable and cords are vulnerable when
bent or twisted, never subject the power cord to
excessive force.
1. Set the main switch①to “O” (off) state before
connecting the power cord.
2. Insert the plugs into the power jack②of the
microscope safely.
3. Plug the power cord ③into the power supply
receptacle④. Make sure the connection is well.
4. Insert aviatic BNC connector plugs⑥into aviatic
BNC connector jack⑤.
★Do use the supplied power cord all the
time. If lost or damaged, select the same
standard cord, please.
★Connect the power cord correctly, to
ensure the instrument is grounded.
①
②
⑧
⑦
⑤
⑥
③
Fig.17

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3. Adjustment Set Diagram
Diopter adjustment ring
Fine focusing
knob
Light path selecting lever
Brightness
adjustment knob
Tension adjustment
collar
Main
switch
Coarse
focusing knob
Interpupilary
distance indicator
Fig.18

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Analyzer
FilterPolarizer Aperture
diaphragm
Condenser
adjustment lever
Field
diaphragm
Fig.19

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4. Adjustment Procedures
4-1 Microscope Base
4-1-1 Turning On the Lamp (Fig.20)
Connect the power supply, then set the main
switch①(shown in Fig.20) on the side of the base to
“－”(on).
4-1-2 Adjusting the brightness (Fig.21)
Turning the brightness adjustment knob ①
clockwise, the voltage raises, and the brightness is
strengthen; whereas turning at the contrary
direction, the voltage declines, and the brightness is
weaken.
◎Using the lamp in a low voltage condition, will
prolong the service life.
4-1-3 Adjusting the Tension Adjustment Collar
(Fig.22)
★ The tension of the coarse focusing knob ②
has already been adjusted properly before
leaving factory.
◎How to adjust the tension of the coarse focusing knob
Turn the tension adjustment collar ①. while revolving at
the direction of the arrow in the figure, the tension of the
coarse focusing knob②is increasing; and if at the
contrary direction, the tension will decline.
If the nosepiece descends on its own, or the specimen
gets out of focus soon even you focus with the fine focus
knob③, It means the coarse focusing knob is too loose.
You should screw the tension adjustment collar down at
the direction of the arrowhead in fig.22 to increase the
tension.
Fig.21
Fig.22
Fig.20
①
①
①②③

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4-1-4 Adjusting the Illumination Set
(Fig.23)
1. Make sure the field of view is bright and
even, and has no filament shadow. If there is
filament shadow, please adjust the condenser
adjustment knob③to proper position.
2. Adjust the field iris diaphragm①and
Aperture iris diaphragm②to a proper
condition, then you can get a clear image.
When searching for the image, you need to
adjust the diaphragm simultaneously until the
image becomes sharpest.
Fig.23
★ Aperture Diaphragm: the aperture diaphragm (iris diaphragm) is not used tocontrol
the brightness but control the aperture angle of the incident ray. The diaphragm is not
adjusted properly until the incident rays are just full of the objective lens. In this case, the
contrast and sharpness of the image will be the best. What to be noticed is that when
changing the objective, you should repeat the adjustment of the aperture diaphragm.
★ Field Diaphragm: used to control the size of the field of view, reduce the reflected rays
of the tube and glare, thus increase the contrast of image. Generally, the field diaphragm is
of proper size when the rays are just full of the field of view of eyepiece.
①
②
③

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①
②
4-2 Mechanical Stage
4-2-1 Placing and Moving the Specimen
(Fig.24 and Fig.25)
1. Place the slide in the center of the stage and use the
stage clips to clamp it gently.
2. The specimen can be moved to desired position by
turning the X-axis knob①and Y-axis knob②.
(stroke: 120mm along X-axis direction, 78mm along
Y-axis direction.)
★Be careful when changing the objectives. If you
finish the observation with the short working
distance objective, and want to change another
one, be careful of not letting the objective touch
the stage center plate or specimen slide.
Fig.24
Fig.25

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4-3 The Viewing Tube 4-3-1 Adjusting the Diopter (Fig.26)
1. Looking through the right ocular with your right
eye, revolve the coarse and fine focusing adjustment
knob to focus on the specimen.
2. Then use your left eye to look through the left
ocular. If the image is not sharp, turn only the
diopter adjustment ring①to focus on the specimen
please.
★There are ±5 diopters on the diopter
adjustment ring. The number which the reticle
on the eyepiece holder points to is your eye’s
diopter graduation.
4-3-2 Adjusting the Interpupillary Distance
(Fig.27)
When observing with two eyes, hold on the left and
right prism holders②,③, turn them around the axis to
adjust the interpupillary distance until the left and right
fields of view coincide completely.
★The scale on the interpupillary distance indicator,
pointed by the dot “.” on the eyepiece holder, shows
the interpupillary distance. (Fig.27)
The range of the interpupillary distance: 48～75mm.
4-3-3 Switching the Light Path (Fig.28)
◎Slide the light path selector lever①by your thumb
to select the light path you need.
◎For the binocular observation, push in the lever
until you hear “clicked” .while for video or
photography, pull out the lever until it reaches the
“clicked” position.
Fig.27
Fig.28
Fig.26
①
①

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Light Path Selecting Lever Brightness Proportion Application
Pushed in
100% used for binocular
observation
Binocular observation
Pulled out
20% used for binocular
observation, and 80% used for
video or photography
Binocular observation and television
\micrography \ video can be performed
simultaneously.
4-3-4 Polarization Observation
1. Mounting the polarizer and analyzer, the detail is available in section 2-7, 2-8.
2. The analyzer hand wheel can be rolled from 0° to 90°. When the field of view becomes the darkest, the
orthogonal polarization position is reached and polarization observation can be performed.
5. Microscope Video and Photography
5-1 Microscope Video
5-1-1 Selecting the Light Path (Fig.29)
★Just used in the trinocular viewing tube.
Pull out the light path selecting lever, until you
hear the “clicked”.
★For the observation of dark specimen, you can
focus it through the binocular at first, then change
the light path.
5-1-2 Installing the Video Set (Fig.30)
1. Loosen the locking bolt①on the trinocular
viewing tube, and take out the dust cap②.
2. Remove the dust cover on the both ends of the
video accessories③, and revolve the CCD/CMOS
set into the screw thread end---the video adapter○
4
with C-mount.
3. Install the video accessories③into the
tri-through port as shown in fig.30, and screw
down the bolt①.
Fig.29
①

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5-2 Microscope Photography
Fig .30
5-2-1 Selecting the Light Path
★Just used in the trinocular viewing tube.
5-2-2 Installing the Photography Set (Fig.31)
1. Loosen the locking bolt①on the trinocular viewing
tube, and take out the dust cap②.
2. Install the photography accessories③into the
tri-through port, and screw down the locking bolts①.
3. Couple the digital photography adapter with the
camera set, then insert the adapter into the
accessories③.
● Before connecting the camera set with the digital
photography adapter, please remove the camera gun
firstly. Pay attention to the camera port type, please.
● To avoid disturbing from the binocular, please place
the viewfinder toward the side of the microscope
when installing the camera set.
● The magnification of photomicrograph = magnification
of objective ×magnification of photography adapter.
Fig.31
1
CCD
Video
2
3
5-1-3 Focus (Fig.30)
Looking through binocular, focus the specimen to
get a sharp image, and then check the image on the TV
or the computer which is connected with the
microscope video set. If it is not clear, please revolve
video adapter○
4until the image is sharp enough.
1
2
3
Camera
Digital
Digital photography adapter
○
4

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★When shooting the micrograph, the shutter release will bring some impact. In order to weaken the
impact and obtain a clear image, you could select a longer time of exposure or decrease the brightness
to have some compensation.
★This explanation is used for Nikon single-lens reflex digital camera.
5-2-3 Focus
Do the binocular observation and focus the specimen firstly. When in microscope photography, do use the
camera viewfinder to focus the specimen. Please refer to the user manual of the photo attachment to obtain
the details.
5-2-4 Adjusting the Color Temperature
◎When shooting the chromophotograph with the sunlight film:
1. Mount the blue filter into the filter bracket.
2. Turn the brightness adjustment knob to the maximal limit, then you can obtain a sunlight illumination.

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6．Technical Specification
6-1 Main Technical Specification
Optical System Infinite Optical System
Viewing Tube Compensation Free Trinocular Tube Inclined at 30; interpupilary distance: 48-75mm
Division ratio: 20% for Binocular Viewing and 80% for Video Viewing & photomicrography
Eyepiece High Point ,Extra Wide Field Eyepiece EW 10X/20
Nosepiece Quintuple Nosepiece
Objective Plan achromatic objective :4×,10×,20×,40×,80×(Optional).
Focusing System Coaxial Coarse and Fine Focusing adjustment ,Vertical Objective Movement,
Coarse Stroke:37.7 mm per rotation, Fine Stroke:0.2mm per rotation
Stage Area: 160×250mm
Mechanical
Ruler (Optional)
X/Y Coaxial Control at Right Hand Side, Moving Range:
120 (width)×78 (Length)mm
Kohler
Illumination Halogen Lamp 6V30W, Preset Center, Intensity Continuously Adjustable
Filter Blue, Yellow, Green and Ground Glass
6-2 Objectives Specification
Magnification Aperture Number
(N.A)
Working Distance
(mm)
Conjugate Distance
(mm)
4X 0.1 25.4 ∞
10X 0.25 11 ∞
20 X 0.4 6 ∞
40 X 0.6 3.7 ∞
80 X 0.9 0.2 ∞