BestScope BS-7020 User manual

Inverted Fluorescence Microscope
BS-7020
Instruction Manual
To ensure correct operation, please read this manual thoroughly before using the
microscope and keep it near the product for easy reference.

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CONTENTS
User notice.....................................................................................................................3
I. Nomenclature .............................................................................................................6
2. Installation .................................................................................................................6
2-1 Installation Diagram..........................................................................................7
2-2 Installing steps ..................................................................................................8
2-2-1 Installing and replacing the lamp ...........................................................8
2-2-2 Installing the lamp house .......................................................................9
2-2-3 Mounting the objectives ........................................................................9
2-2-4 Installing the stage lengthen splint and the mechanical ruler.............10
2-2-5 Installing the stage inserted plate........................................................10
2-2-6 Installing the eyepiece..........................................................................10
2-2-7 Installing the color filters......................................................................11
2-2-8 Connecting the power cord..................................................................13
3. Controls....................................................................................................................14
4. Adjustment &Operation...........................................................................................15
4-1 Transmitted Light Adjustment ........................................................................15
4-1-1 Turning on the lamp .............................................................................15
4-1-2 Adjusting the brightness.......................................................................15
4-1-3 Adjusting the tension of the coarse adjustment knob.........................15
4-1-4 Using color filter ...................................................................................15
4-1-5 Using the aperyure diaphragm.............................................................15
4-1-6 Removing the condenser lens ..............................................................15
4-2 Stage ...............................................................................................................18
4-2-1 Placing the specimen............................................................................18
4-2-2 Moving the specimen..........................................................................18
4-3 The viewing tube ............................................................................................19
4-3-1 Adjusting the diopter............................................................................19
4-3-2 Adjusting the interpupillary distance...................................................19
4-3-3 Selecting the light path.........................................................................20
4-4 Adjustment of Power Supply Unit ..................................................................22
4-4-1 Power supplier connection...................................................................22
4-4-2 Controls on Power Supply Unit ............................................................22
4-4-3 Centering the Mercury Burner .............................................................23
5. Phase Contrast Observation ....................................................................................24
5.1 Name of components .....................................................................................24
5-1-1 Phase contrast objective ......................................................................24
5-1-2 Phase contrast slider ............................................................................24
5-2 Installation and usage.....................................................................................24
5-2-1 Installing the phase contrast slider ......................................................24

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5-2-2 Centering the light annulus..................................................................25
6. Microscope photography and video........................................................................26
6-1 Microscope video............................................................................................26
6-1-1 Selecting the light path.........................................................................26
6-1-2 Installing the video set .........................................................................26
6-1-3 Focus.....................................................................................................26
6-2 Microscope photography ...............................................................................27
6-2-1 Selecting the light path.........................................................................27
6-2-2 Installing the photography set .............................................................27
6-2-3 Focus.....................................................................................................27
6-2-4 Adjusting the color temperature..........................................................27
6-3 Digital photography ........................................................................................28
6-3-1 Selecting the light path.........................................................................28
6-3-2 Installing the photography set .............................................................28
7. Specifications ...........................................................................................................29
7-1 Main specifications.........................................................................................29
7-2 Objective Specifications..................................................................................30
7-3 Fluorescence mirror block ..............................................................................30
8. Troubleshooting.......................................................................................................33
9. Product Standard .....................................................................................................31

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User notice
1. Application
BS-7020 Inverted Biological microscope is the dedicated microscope for biological and medical
areas, applied in the field of microscopic measurement, health agencies, laboratories, research
institutes and universities and other units for biology, genetics, immunology, chemistry,
environment protection, oceanography, pharmacology, bacterial observation, education and
professional studies.
2. Safety Precaution
1. Do not keep the instrument in a direct sunlight, high temperature or humidity, dusty
and easy shaking environment. Make sure the stage is plane, horizontal and stable
enough.(Weight: about 11.5 kg)
2. When moving the microscope, please hold the instrument by the lower side of the
observation tube④and the illumination column②. (Fig.1）
3. If the bacterium solution or the water splash to the stage, objective or viewing tube, set
the main switch to off state and unplug the power cord. Then wipe away any liquid.
Otherwise, the instrument will be damaged.
4. When working, the lamp house on the top of the arm③(Fig.1) will become very hot,
be sure there is enough room around the lamp house (especially the top side for
cooling).
Fig.1

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5. Before replacing the lamp bulb or fuse, turn the main switch①to the “O”(off) position,
then cut off the power. If the lamp is on, or soon after it has been turned off, it is hot
and will cause serious burns, please do the replacement after it cool down completely.
★Specified lamp: the halogen lamp 6V30W (PHILIPS5761)
6. Earth this instrument to prevent the lightning strike.
7. Use the specified power cord, please.
8. The product should stored in a shady location and no acidic gases, alkalis, organic
solvents and other hazardous materials surrounding, the storage period is usually not
more than 6 months.
9. ★always ensure that the grounding terminal of the microscope and that of the wall
outlet are properly connected. If the equipment is not grounded, we can no longer
warrant the electrical safety performance of the equipment.
3. Maintenance and Storage
1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges,
wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%).
★Since solvents such as ether and alcohol are highly flammable, they must be handled carefully.
Be sure to keep these chemicals away from open flames or potential source of electrical
sparks---for example, electrical equipment that is being switched on or off. Also remember to
always use these chemicals only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other than the
glass components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted
neutral detergent.
3. be careful not to spill any liquid –such as a culture solution –on the unit. if you do spill
anything, immediately set the main switch to off and unplug the power cord. then wipe away any
liquid on microscope.
4. Do not disassemble any part of the microscope as this could result in malfunction
or reduced performance.
5. If no objectives are mounted, be sure to cover the objective mounting threaded positions on
the revolving nosepiece to prevent any dust and spilled culture solution
from getting on the lenses inside.
6. When not using the microscope, keep it covered with a dust cover. Make sure
the lamp socket is cool before covering the microscope.

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4. Safety Symbols
Symbol
Explanation
Indicate that the surface becomes hot, and should not be touched
with bare hands.
Before use, carefully read the instruction manual. improper use
could result in personal injury to the user and/or damage to the
equipment.
Indicate that the main switch is ON.
Indicate that the main switch in OFF.
VERSED BIOLOGICAL MICROSCOPE
INVERTED BIOLOGICAL MICROSCOPE

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I. Nomenclature
INVERTED BIOLOGICAL MICROSCOPE
BS-7020 Inverted Fluorescence Microscope
Lamp House
Reflected Fluorescent Mirror Block
Fig.2
Lamp house
Filter holder
Eyepieces
Phase contrast slider
Illumination column
Condenser
Mechanical ruler
Auxiliary stage
Objective
4x, 10x, 20x, 40x
Main switch
Light intensity control knob
Main frame
35/54 mm Petri dish holder
Terasaki holder
Observation tube
Protection barrier
Stage

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2. Installation
2-1 Installation Diagram
The following figure shows the installation sequence of the components. The number in the
figure shows the installation sequence.
Before installing, be sure every component is clean, do not score any parts or glass
surface.
Keep well with the supplied S1.5 and S2 hexagon wrench. When changing the components,
you will need it again.
⑧
Fig.3
④

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2-2 Installing steps
2-2-1 Installing and replacing the lamp (Fig.4)
Please use the specified halogen Lamp 6V30W.
1. Hold to the bulb ①after you wrap it with gauze or other protection materials, then depress
the plugs②into the jack④on the lamp house, ensure the filament and the
bolt③are in a same level.
2. Replacing the lamp when using or soon after
When using, or soon after it is turned off, the lamp, the lamp house and nearby parts will be
very hot and will cause serious burns. Please turn the main switch to “O” (off), pull up power
plug, and make sure the bulb, the lamp house and periphery are all cool. Then, you can do
your replacement.
Please insert the bulb gently, or it will be damaged by excessive extrusion.
Do not touch the Halogen bulb with your bare hands. It will shorten the service life or cause
it to burst. If you leave fingerprints on the surface carelessly, clean it with a dry soft cloth.
Fig.5
Fig.4

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2-2-2 Installing the lamp house (Fig.5)
Keep the BNC connector plugs③and the lamp house pin②in line, and keep the
bolt①and the condenser jack④in line, too. Then push the lamp house into the
illumination unit gently until they are against each other.
2-2-3 Mounting the objectives (Fig.6-7)
1. Turn the coarse adjustment knob①as Fig.6 shows until the revolving nosepiece is set at its
lower limit.
★For ensuring the safety of the instrument during transportation, the nosepiece is located in
the lowest position and the tension adjustment collar②is adjusted to an appropriate tension
while leaving the factory.
2. Screw the lowest magnification objective onto nosepiece from the nearside, then turn the
nosepiece clockwise, mount other objectives according the magnification sequence of low to
high.
◎Mount objectives in this way will make the change of magnification to be very easy in using.
◎It also can install the objective through the stage opening.
★Clean the objective regularly, the objective used in the inverted microscope is very sensitive
to dust.
★Be sure to cover any unused threaded positions with the objective caps to prevent dirt and
dust from getting inside.
★When operating, use the low magnification objective (4X or 10X) to search and focus the
specimen at first, then use higher magnification objectives if necessary.
★When replacing the objectives, slowly turning the nosepiece until you hear “clicked”, that
means the objective enter into the right position—center of the light path.
Fig.7
Fig.6

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2-2-4 Installing the stage lengthen splint and the mechanical ruler (Fig. 8)
2-2-5 Installing the stage inserted plate (Fig.9)
INVERTED BIOLOGICAL MICROSCOPE
2-2-6 Installing the eyepiece (Fig.10)
1. Remove the cap of the eyepiece tube①.
2. Insert the eyepiece②into its tube③until they are against each other.
3. screw tightly the bolt③with provided S1.5 wrench to keep the eyepiece from dropping out.
◎Stage lengthen splint can be installed at either
side of the stage to enlarge the work surface.
But you can’t install the mechanical ruler
together at the same side.
◎Generally, the mechanical ruler will be installed
at the right side for comfortable adjustment.
1. Installing the stage lengthen splint
First, Screw the fixed bolt① on to the splint,
then mount it on to the stage from right or left
below, screwing down the bolt until it stay
hard.
2. Installing the mechanical ruler
Please install the ruler in the same way as the
stage splint.
1. When using the glass stage ①, there is no
special requirement, you just need to place it in
a plane.
2. Install the stage inserted plate on to the stage
opening.
◎Turn the disk, let the V nick face the user, so the
recognition of the objective will become easier.
Fig.8
Fig.9

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2-2-7 Installing the color filters (Fig.11)
INVERTED BIOLOGICAL MICROSCOPE
2-2-8
Fig.11
Let the filters cool down sufficiently before replacing
them. Take out the filter holder①and insert the
required filters②.
Push the filter down to the bottom as shown in Fig.11
③so that it does not tilt. If the filter is inclined or is
not pushed down to the bottom④, it may fall off the
filter holder.
◎More than one filter can be stacked in the filter holder.
You can mount as many as you like, as long as the total
thickness does not exceed 11mm.
Mounting the Mercury Burner
(Fig.12 & fig.13)
1. Loosen the burner socket clamping screw ①,
and remove the burner socket. (fig.12)
2. After removing the plastic backstop ④,
securely insert the + pole (the wide head) of the
specified mercury burner②to the lower
terminal first and then the –pole(the thin head)
to the upper terminal, then tighten the two
socket clamping screws③. (fig.13)
Fig.12
Fig.10
①

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Fig.15
● Be sure to use a 100W HBO spherical mercury
burner.
●Be sure to mount positive pole(the wide head)
before the other , or the damage to the burner
may occur.
● Never subject the burner to excessive force
when mounting the Mercury Burner.
● Be careful and avoid leaving fingerprints or dirt
on the mercury burner. Attached stain may
cause distortion in glass which could result in a
ruptured burner. If stained, wipe it a way gently
with clean gauze.
★To prevent any hazard, always turn the main
switch on the power supply unit to “O” (OFF),
unplug the power cord plug from the mains
outlet, and wait for at least 10 minutes before
replacing the burner.
2-2-9 Lamp house, power supply unit and
cord connections (Fig.14＆15)
1．Connect The lamp house to the access tube,
and then tighten the lamp house with the
ring④, as shown in fig14.
2．Plug the connector from lamp house securely
into the connector⑥on the power supply unit
and make sure the cord is correctly connected.
3. Connect the two ends of power cord to the
power supply unit and wall outlet respectively.
(Make sure that the main switch⑦of the
power supply is set to “O”（OFF）before
connecting cables.)
● Verify that the voltage and the frequency of the
AC mains outlet matches the setting of the
voltage and the frequency switches on the rear
of the power supply units(fig.15) and improper
setting may degrade burner performance , or in
the worst case(although very rare), cause the
burner to explode.
● It is better to use the supplied power cord and
the same type power cord should be used if
you lose or damage the old one.
④②③
Fig.14
Fig.13
⑦
⑤
Voltage
⑥
Frequency
④

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2.2.10 Attaching the protection barrier, stage
center plate and Fluorescence mirror switching
lever (Fig.16)
1. Extend The light shielding plate into the lower part
of the platform, then tighten the two clamping screws②.
2. Fit the stage center plate into the opening on the stage.
3. Screw the Fluorescence mirror switching lever③into
the reflected fluorescence illuminator unit below the nosepiece.
2-2-11 Connecting the power cord (Fig.17,18 &19)
2-2-12 Replacing the fuse (Fig.17-19)
★Cables and cords are vulnerable when bent
or twisted. Never subject them to excessive
force.
1.Make sure that the main switch of the power supply is set
to “o”(OFF)before connecting cables.(Fig.17)
2.Connect the plug④of the illumination column firmly to
its jack⑤on the rear of the microscope.(Fig.18)
3. Connect one end of the power cord②into its connector
③ on the rear of the microscope.(Fig.18)
4. Connect the other end of the power cord②to a wall
outlet⑥.(Fig.19)
★Do always use the supplied power cord. If lost or
damaged, select the same standard cord, please.
★Connect the power cord correctly, to ensure the
instrument is grounded.
Fig.19
★Do remember to turn the main switch① to the state of “O”
(off) before replacing the fuse, and unplug the power
cord②. Rotate the fuse kits out of the holder by the
“--”type screwdriver, replace a new fuse, then rotate back
to the holder again.
★Fuse rating: 250V, 1A.
Fig.16
Fig.17
②
③
Fig.18

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3. Controls
Fig.20
Light Annulus Centering
Bolt
Aperture iris diaphragm
lever
Filter holder
Fine focusing knob
Coarse focusing
knob
Tension adjustment collar
Phase contrast slider
Optical path switching knob

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4. Adjustment & Operation
4-1 Transmitted Light Adjustment
4-1-1 Turning on the lamp (Fig.21)
4-1-2 Adjusting the brightness (Fig.21)
4-1-3 Adjusting the tension of the coarse
adjustment knob (Fig.22)
★Be sure to use the tension adjustment ring①
to adjust the rotation tension of the coarse
adjustment knob.
Adjustment procedure:
◎How to adjust the tight tension
Turning the tension adjustment ring ① with
your finger by counter-clockwise in the figure,
the tight tension of the coarse focus knob② is
increasing；And if at the contrary direction, the
tight tension will decline.
If the nosepiece dropped automatically, or the
specimen defocused soon even you focus with the
fine focus knob③. It means the coarse focus knob
is too loose, you should screw it down at the
direction shown by the arrowhead in the Fig.22.
Connect the power supply, turn on the main
switch①(shown in Fig.21)which on the
bottom side of the base to “－”(on).
Turning the brightness adjustment knob②
clockwise, the voltage raise, and the brightness
strengthen; Whereas turning at the contrary
direction, the voltage decline and the brightness
weaken.
◎Using the lamp in a low voltage condition
will prolong the service life.
Fig.21
Fig.22

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4-1-4 Using color filters (Fig.23)
◎using appropriate filters according to the purposes allows you to observe and photograph
specimens more effectively. Particularly, the use of the LED filter is recommended in
observation and photomicrography because it renders more neutral colors.
◎More than one filter can be stacked in the filter holder (filter diameter: 45mm maximum;
thickness of stacked filters: 11mm)
Fig.23
XDINVERTED BIOLOGICAL MICROSCOPE
-202 型倒置生物显微
Filter
Application
IF550
Monochrome contrast filter (green)
LBD
Color temperature conversion filter (for observation and
photomicrography)
SIF800
Exposure time compensation in photomicrography
Fig.24
70-80%
30-20%

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4-1-5 Using the aperture diaphragm (Fig.24)
4-1-6 Removing the condenser lens (Fig.25)
BS-7020 型倒置生物显微
◎the aperture iris diaphragm determines the numerical aperture of the illumination
system in bright field observation.Only when the numerical aperture of the objective
and the illumination system being matching, you can obtain the higher image
resolution and contrast, and the increased depth of focus, too.
● Checking the aperture iris diaphragm
Remove the eyepiece when necessary (and inset the centering telescope if you have
one), then look into the eyepiece sleeve; you will see the field of view as shown in
Fig.24.now adjust the aperture iris diaphragm lever as required.
● Generally, when observing a dyed specimen, set the aperture iris diaphragm② to 70% to
80% of the N.A. of the objective①in use. However, when observing a culture specimen,
which is not dyed, set the aperture iris diaphragm lever toward“
” .
To provide more working distance, turn the condenser’s lower section ① and remove it.
The height of Petri dish can be up to 150mm.
★When you do this, however, keep in mind that proper illumination cannot be achieved.
Remove the condenser lens only when using a large culture vessel.
Fig.25

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4-2 Stage
4-2-1 Placing the specimen (Fig.26& Fig.27)
4-2-2 Moving the specimen
Put the specimen in the center of the stage.
★ to obtain the best image effect, please
select the containers, such as culture dish and
culture bottle, with the bottom thickness of
1.2mm, and the same thickness is also required
for the specimen slide.
◎Using the Φ35mm culture dish
You can lay a Φ35mm culture dish on the stage
directly by using the standard center board①of
the stage.
◎Using the mechanical ruler
1. When using the 96bit or 24bit micro-titration
board, please fasten it tightly by the stage clips
②.
2. When fastening other model boards, please use
the following supplied brackets with mechanical
ruler:
⚫Terasaki bracket⑦for Terrasaki board.
⚫Culture dish bracket⑤forΦ35mm culture dish.
⚫Slide bracket⑥for slide and Φ54mm culture dish
Turning the transverse knob③and lengthways
knob④, move the specimen to the required
position.
(Movement Range: 120 (width)×78 (Length) mm)
Fig.27
Turn the X-axis and Y-axis knobs of the mechanical stage or move the specimen directly by
hand.
★Be careful when changing objectives. When objectives are switched after observing the
specimen with an objective with short working distance, the newly selected objective
may interfere with the stage center plate or Petri dish holder. Turn the coarse focusing
knob slightly to lower the objective properly for that.
Fig.26

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4-3 The viewing tube
4-3-1 Adjusting the diopter (Fig.28)
4-3-2 Adjusting the interpupillary distance
(Fig.28-29)
1. Look into the right ocular by your right eye, then
revolving the coarse focus knob to focus on the
specimen.
2. Then use your left eye to look into the left
ocular. If the image is not sharp, just use the
diopter adjustment ring①to adjust please.
★There are ±5 diopter in the adjustment ring.
The number which the reticle on the eyepiece
holder pointed is your eye’s diopter graduation.
1. While looking through the left eyepiece with
your left eye, turn the coarse and fine focus
adjustment knobs to bring the specimen into
focus. While looking through the right eyepiece
with your right eye, turn only the diopter
adjustment ring ①to focus on the specimen.
★There are ±5 diopter in the adjustment ring.
The number which the reticle on the eyepiece
holder pointed is your eye’s diopter graduation.
Fig .28
While looking through the eyepieces, move
both eyepieces until the left and right fields of
view coincide completely.
★The reticle on the interpupillary distance
indicator③, pointed by the spot “.” ②on the
eyepiece holder, shows the scale of the
interpupillary distance. (Fig.28)
The range of the interpupillary distance:48～
75mm.
Fig.29