BestScope BS-2080D User manual

BestScope International Limited
Motorized Microscope
With Auto-Focus System
User Manual
This manual is for BS-2080D microscope. To ensure the safety, obtain optimum
performance and to familiarize yourself fully with the use of this microscope, it is
recommended strongly that you study this manual thoroughly before operating the
microscope.

1
Safety Precautions
Observe safety precautions
Periodic inspection
Not use when the microscope breaks down
Disposal of abnormal situation
Warning symbols
Warning symbols
Prohibition symbols
Designation Symbol
The product designed is conformation of the requirements about
safety. Incorrect operation is dangerous, maybe cause fire, electric
shock or other serious personal injury
To avoid such accidents, please follow the instructions
please read the warning noteson page 2 carefully
For the long-term safety , regular inspection is
recommended . Contact your product supplier for detail
strongly
Contact your product supplier for help.
Abnormal noise,
heat, smoke, or a
burning odor
(1) Shutdown the power.
(2)Unplug all power cords and cables
(3) Contact your product supplier for help
These symbols will be used on
microscope and throughout the whole
manual . So fully understand the
meaning of the signs is necessary
before you continue to read the
manual. Make sure that you observe
all warnings given.
the
WARNING
Indicates that errors in operation may
lead to death or serious injury
Indicates that errors in operation may
lead to fire, shock, or equipment
damage
DANGER
CAUTION
FIRE
ELECTRIC SHOCK
NO HUMIDITY
PROHIBITION
NO WET HANDS
NOT
DISMANTLING
INSTRUCTION PULL THE PLUG

2
Not take the following precautions may result in fire,
electric shock, other serious injury or even death
Please use the special power adapter provided, use other
power adapter will not ensure the safety of persons and
equipment.
DANGER
FIRE
ELECTRIC SHOCK
PULL THE PLUG
when the following exceptions occur, please shutdown the
power at once and pull the plug from the AC outlet:
(1) abnormal noise, heat, smoke or a burning odor
(2) water inside into the equipment
(3) foreign body inside into the microscope
(4) the microscope is dropped or the cabinet damaged
INSTRUCTION
Any of the following exception occurs ,
please cut off the electricity supply at once
CHECK THE ELECTRICAL SOURCE
Don’t let the water inside into the equipment
or stain the surface of the equipment
Doing so may cause fire, electric shock or damage microscope
NO HUMIDITY
Do not use the microscope under humidity
and dusty environment
Doing so may cause fire, electric shock or damage microscope
Prevent damage to power cord
When disconnect the AC power cord, do not pull the power
cord, please grasp the plug and pull it out
Clean up the impurity attached to the pin of
the power plug
INSTRUCTION
INSTRUCTION
If the surface of the power plug pins and nearby parts have
dust or metal Particles, please pull the plug from the AC outlet,
then wipe them with a dry cloth
Do cut off the power
microscope
before cleaning the
Before cleaning the lamp or the microscope, Be sure that you
had turned off the power and disconnected the AC power cord.
NOT
DISMANTLING
NOT
DISMANTLING
Doing so may cause fire, electric shock or damage microscope
Do not refit the microscope
Do not disassemble the microscope
Inspection, maintenance or repair of microscopes require
specialized technicians, if you want, please contact the product
supplier.
All the lenses have been well checked and adjusted. It is
forbidden to disassemble them yourself.
The nosepiece and coarse/fine focus unit have a compact and
precise mechanical structure; please don’t disassemble them
by yourself.
INSTRUCTION
INSTRUCTION
Do pull the power plug from the AC wall
outlet when not use the microscope for a
long time
For safety, be sure the main switch is in “O”(off) state before
replacing the halogen lamp or the fuse, then cut off the power,
and do the operation after the lamp bulb and the lamp house
completely cool down.( Specified: Halogen Lamp24V/100W)
Do cut off the power
lamp
before replacing the
To connect the power plug and the AC power outlet too long
time will lead to fire.
WARNING Not take the following precautions will lead to electric shock,
personal injure or the surrounding objects damage
NO WET HANDS PROHIBITION
PROHIBITION
PROHIBITION
INSTRUCTION
INSTRUCTION
INSTRUCTION
INSTRUCTION
Do not plug the power plug with wet hands
Otherwise will cause electric shock.
Do not put too much pressure on the
microscope.
Doing so may cause the product to overturn or fall, causing
personal injury.
Do not place the product in an inclined or
unstable place
Doing so may cause the product to overturn or fall, causing
personal injury.
Keep the microscope clean, wipe dust
regularly
Do not use organic solvents to clean the non-optical
microscope parts. To clean them, use a neutral detergent.
Lens stains such as fingerprints, grease, use a clean soft
cotton cloth, lens paper or cloth dipped in pure alcohol
(ethanol), xylene or ethyl to gently wipe away. (Note that
xylene and alcohol are highly flammable, do keep them away
from the fire or potential sources of electrical sparks, and use
them in a drafty room as possible as you can.)
Do not place the power cord near heat
sources
Doing so may lead to power lines cover melted, causing fire or
electric shock.
Pus h t h e powe r p l ug int o t h e sock e t
completely.
If the plug is not fully inserted into the socket, it likely to
generate heat or the accumulation of dust between them, that
may cause a fire and Contacting the plug pins may cause
electric shock.
Place the microscope in a good working
environment
Do keep the microscope out of direct sunlight, high temperature
or humidity, dusty, and easy shaking environment, Make sure
that the loading platform is smooth, horizontal and firm enough.
When running, the lamp house and nearby parts will be very
hot. Please ensure there is enough cooling room for them.
Open the box and move the microscope
carefully
Open the box caref ully to prevent the lens and other
accessories dropping to ground and being damaged. When
moving the microscope, please use two hands to grip with the
two sides of the microscope body. Otherwise, the main body
may fall, causing personal injury.
PROHIBITION
PROHIBITION

Contents
Safety Precautions ..............................................1
Danger ......................................................1
Warning .....................................................2
1. Components.............................................. ......... .. .. .4
2. Installation .....................................................6
2.1 Installation Diagram .........................................6
2.2 Installation Steps ...........................................7
3. Adjustment ...................................................13
4. Observation Steps ..............................................14
...
5. Adjustment Operation .........................................15
6. Control Box Menu Setting .......................................18
6.1 Main menu .....................................................18
6.2 Sub Menu ......................................................................................... .18
7. Technical Specifications ........................................20
8. Troubleshooting Guide .........................................21
8.1 Optical system ...............................................21
8.2 Mechanical system.............................................22
8.3 Electrical system .............................................22
3

1. Name of Components
FrontView
4
Eyepiece (Ocular)
Nosepiece
Objectives
Lead Screw Platform(Stage)
(With Communication Interface )
Main Body
Camera
Video Adapter
Control Box
Rear View
Trinocular Viewing Unit
Computer Communication
Interface
Communication Interface
(Serial Port)
Power Outlet
Power Switch
Communication Interface
(Parallel Port)
Power Switch
Signal Transmission
Interface
Platform Communication
Interface
Communication Interface
(Serial Port)
Communication Interface
(Parallel Port)
Power Outlet
Lamp House
Lamp connector

Control Panel
5
1. Name of Components
OK
Cancel
RTF
AF
RTF OFF
RTF ON
LUM ZAxis OBJ
Display Panel
P l a t f o r m C o n t r o l
Touchpad
Z-axis Lift Wheel
F u n c t i o n
Buttons
A u t o - F o c u s
Button
R e a l - F o c u s
Button
B r i g h t n e s s
Adjust Button Z - a x i s L i f t
Button
O b j e c t i v e s C h a g e
Button
R T F S t a t u s
Indicator
Lens Change Button: change the objectives at
clockwise or counterclockwise .
Auto-Focus Button: Focus automatically once.
Real-time Focus Button: Control the real-time
focus control function ON or OFF. IF ON,
moving specimens, microscope automatically
focus the specimens all the whole time.
RTF Status Indicator: Displays the current state
of real-time focus function.
Function Buttons: Menu settings buttons.
down
Display Panel: Displays the current status of the
platform and the information ofthe microscope System
. The current platform position, lens position and the
system brightness. When insert the system setting
status, it displays system setup parameters.
Z-axis Lift Wheel: roll the wheel to control the Lead
screw platform toup and down. (Z-axis Movement).
Platform
Control TouchPad: Use your fingertip moving on the
Touchpad. Simultaneously, the Microscope stage will
be moving as your fingertip do. (X-axis and Y axis
movement).
Brightness adjustment Button: Adjust the brightness .
Z-axis Lift button: Z-axis's up and

2. Assembly
2.1 Assembly Diagram
6
The following figure shows the installation sequence of the components. The number in the
figure shows the assembly steps.
Before installing, be sure every components is clean, do not score any parts or glass
surface.
Keep well with hexagon wrench provided. When replacing the components, you will need it
again.
Lamp House
Main Body
Stage Support Device
Objectives
Lead Screw
Platform (Stage)
Specimen(Sli ce)
Holder
Trinocular Viewing Unit
Video Adapter
Camera
Eyepieces

2.2 Assembly Steps
2.2.1 Installing Stage Support Device
Before installing the device, be sure
to adjust the coarse focus knob.
Make the guide board (see figure
1)down to the limit position, so you
can install the stage support device
easily.
Hold on the stage support device
(figure 2), place it from the top of the
guide board (figure 1), let the device
(figure2) falling free until it reach
the limit position. Use the hexagon
wrench to screw down the screw
and locking block , make the
stage support device (figure1) and
the guide board fixed together. (see
figure 3)
fig. 1
fig. 2
7
fig. 3
2. Assembly
The lead screw platform and the stage
support device have been adjusted
horizontally and fixed together before leaving
factory. Please don’t disassembly it unless
necessary, otherwise ,it will affect the
observation precision of the microscopy.

2.2 Assembly Steps
2.2.2 Installing the Trinocular Viewing Unit
fig. 4
2.2.3 Installing and Replacing the Lamp
fig.5
8
2. Assembly
1. Loosen the fixed bolt ① on the
microscope main body, but do not let it
① fall out.
2. Insert the trinocular viewing unit into
the microscope head (Figure4), turn to a
proper position, let the two eyepiece
tubes toward the front, then use the
hexagon wrench screw down the fixed
bolt ① to fix ( See Figure 4 ).
Only allow to use the specified halogen
Lamp 24V100W ①.
Do not touch the Halogen bulb with
your bare hands. It will shorten the service
life or cause it to burst. If you leave finger
marks on the surface carelessly, clean it
with a dry soft cloth.
1. Remove the lamp House cover
Loosen the fixed bolt ① of the lamp house
cover by hex wrench. Pull up and remove
the Lmap House cover as shown by ②.
2. Install the bulb
Hold the bulb with gauze or other protection
materials.Press down the bulb deadlever
③ . Insert the bulb's pins fully into the jack
of the lamp house ④ . Then back the bulb
deadlever into its original position to fix the
bulb.
3. Replacing Lamp when using or soon after
When using, or soon after it is turned
off, the lamp, the lamp house and nearby
parts will be very hot and will cause serious
burns. Please turn the main switch on “O”
(off), pull out power plug, and make sure the
bulb, the lamp room and periphery are all
cool. Then, you can do your replacing.


2.2 Assembly Steps
2.2.4 Installing the Lamp House
Fixed Bolt
fig. 6
fig. 7
2.2.5 Installation the lead screw platform
9
fig. 8
2. Assembly
Adjust the coarse focus knob. Make
the stage support device (see figure
2)down to the lowest position,so
you can install the lead screw
platform ① easily.
Hold on the lead screw platform ①
(figure 8),place it from the top of the
Stage Support Device (figure 2).
push the lead screw platform gently
until it reaches the limit position
with Keeping the stage support
device (figure2) in level.Use the
hexagon wrench screw down the
fixed bolt ② , make the lead screw
platform and the stage support
device fixed together(see Figure
8).
Keep the lamp house in line with the
jack on the back of the microscope,
then pushing the lamp holder into the
illumination kits gently until they are
against each other. Use the hexagon
wrench screw down the fixed bolt(see
figure 6),then the installation is finished
(figure 7).

2.2 Assembly Steps
2.2.6 Installing objectives
fig. 9
fig. 10
If the objective mounted holes on
the nosepiece is not in use ,
please cover them by the lens
cap to prevent dust or dirt into the
internal.
100 × objective is a oil lens,
when use, please drop some
cedar oil on the specimen first, so
y o u c a n a c h i e v e t h e b e s t
observation
2.2.7 Installing the Eyepieces
10
fig. 11
2.2.8 Installing the Video Adapter
2. Assembly
1. Adjusting the coarse focus knob
until the support device of the stage
reaches its low limit position.
2. Mount the objectives in the right
place as the label shows.
Installing objective this way will
make the change of magnification to
be easier during using.
Clean the objective regularly, for
lens is susceptible to dust.
W h e n o p e r a t i n g , u s e 1 0 ×
magnification objective to search
and focus specimen firstly, then
replace with higher magnification
objective if necessary.
1-4X 2-10X 3-20X 4-40X 5-100X
Insert the eyepiece ① into the
eyepiece tube ② of the trinocular
viewing unit until they are against
each other as shown in Fig.11.
Insert the video adapter ③ into the
trinocular viewing unit, then screw
down the bolt④ to fix it. The result
is showing in figure 11.
1
2
3
4
5

2.2 Assembly Steps
fig. 12
fig. 13
11
2. Assembly
2.2.10 Installing Camera
Insert the Camera into the
video adapter (see Figure 12)
①
②
2.2.11 Installing Specimen Bracket
Place the specimen Bracket ① on
the opening of the lead screw
platform ② (Figure 13).

2.2 Assembly Steps
12
fig. 14
PC
PC
Adapter
AC POWER
6
7
2.2.12 Connection Cable and Power Cord
The cable and cords are vulnerable
when benting or winding, never
subject the power cord to
excessive force.
Turn the main switches of the main
body and the control box ① to “O”
(off) state before connecting the
power cord
1. Use the specified cable to
connect the platform
communication interface as shown
in ①.
2.Use the specified cable to
connect the communication
interface (parallel port) as shown
in ②.
3. Use the specified cable to
connect the communication
interface (serial port) as shown in
③.
4. Use the specified cable to
connect the camera and the control
box into the computer separately
as shown in ④.
5. Plug the power cord firmly into
the socket of the main body and the
control box as shown in ⑤ .
6. Insert the lamp house Firmly into
the main body as shown in ⑥.
Do use the specified power cord all
the time.
Do not plug anything but power
cord into the outlet.
Do use the three-phase power
with correct ground connecting . Or
it will be dangerous.
Do not touch the cables and power
cords to lamp house or nearby.
Doing so may lead to their cover
melted, causing fire or electric
shock. please keep them away
from the lamp house to avoid such
accidents.
7. Fixed the trinocular viewing unit
into the main body as shown in ⑦.
2. Assembly

13
Power Switch
Interpupillary Distance
Scale
Optical Switch Push Rod
Platform Control TouchPad
Diopter Ring
Swing Condenser
Z-axis Lift Wheel
Operate Keyboard
(see the name of components)
Power Switch
(see the name of components)
Condenser Adjustment
Fine Focus Knob
Coarse Focus Knob
Tension Adjustment
Collar
Field Diaphragm
3. Adjustment

OK
Canc el
RTF
AF
LUM ZA xi s OBJ RTF OF F
RTF O N
14
4. Observation Steps
1. Install the microscope by following
the assembly steps.
2.Turn on the power switches on the
main body and the control box
separately.
Note: After the power connecting ,
the microscope will automatically
ca r ry o ut a se rie s of de v ic e
initialization, which will take some
time. It's length determined by the
position of each axis(including the
screw platforms, Z axis, converter)
b e f o r e t h e i n i t i a l i z a t i o n . I n
generally, the initialization will
complete within a minute, be
patient please.
3. Adjust the brightness adjust button
t o o bt a i n a n a p pr o pr i at e
brightness。
4 . P l a c e t h e s p e c i m e n o n t h e
specimen holder。
5. After the initialization finished ，the
n o s e p i e c e s w i t c h t h e 1 0 ×
objectives into the optical path
automatically
6. Turn the eyep iece tube of
trinocular viewing unit to adjust
the interpupi l lary distance (see
Figure 16)
7. Adjust diopter adjustment ring as
shown in Figure 16.
8. Slide on the platform control
tou chpad gent ly , contro l th e
platform to move, let the speciate
go to the desired position.(see
Figure 17).
9. Press the auto-focus button to
focus automatically (see Figure
17).
fig. 15
fig. 16
fig. 17
Z-axis Lift
Wheel
Platform Control
Touchpad B r i g h t n e s s Adjust Button
Auto-Focus
Button
Power Switch

OK
Cancel
RTF
AF
LUM ZAxis OBJ RTF OFF
RTF ON
15
5.1 Turn on the power
5.2 Adjust The Brightness
5.3 Adjust The Tension Adjustment Collar
5.4 Place The Specimen
After the initialization, the specimen
holder should be in the center of the
optical path or nearby. If not find in the
c e n t e r , o r p l a c e t h e s p e c i m e n t
inconveniently, please slide the "platform
control touchpad" (see Figure 17) and
scroll "Z-axis scroll down" (see Figure 17)
to m ove the h old er in to a s uit abl e
position,then input the specimen (see
Figure 19).
If the control not open, you can use the
coarse and fine focus knob to adjust the
platform'lift, turn the X-axis and Y-axis
screw knobs to move the platform.
Press the "Z-axis lift button" gently to
adjust the Z-axis:"↑" to make Z-axis up, "↓
" to make Z-axis down, .
Press the "Objectives
change button" to switch the magnification
of the objectives.There two buttons,both
t h e y c a n s w i t c h , j u s t i n d i f f e r e n t
direction.(see Figure 20).
Be careful when switching the objectives.
Especially after a short working distance
observation,the objective may collide with
the specimen.
The Tension Adjustment Collar
Wrench
fig. 18
fig. 19
fig. 20
Slice（ ）specimen
Turn the power switch of main body and
control box on to "Ⅰ " (open) position
separately , connect the power and wait the
micros co pe to finis h the initia li zatio n
patiently as shown in figure 15.
Press the "brightness adjustment button"
gently: "↑" to make light brighter and "↓" to
make light dim. please adjust the light to an
appropriate brightness. ( Figure 17 ).
The tightness of the tension adjustment
collar has been adjusted at the factory, if too
loosing ( that means the lead strew stage
will glid down automatically by its own
weight),turn the tension adjustment collar
turn with a wrench in an anticlockwise
d i r e c t i o n u n t i l t h e t i g h t n e s s i s i n
order(Figure18).
O b j e c t i v e s
change button
Z-axis lift button
5. Adjustment Operation

OK
Cancel
RTF
AF
LUM ZAxis OBJ RTF OFF
RTF ON
16
5. Adjustment Operation
fig. 21
fig. 22
Auto-Focus
Button
RTF Status
Indicator
Real-Time
Focus Button
fig. 23
5.5 Adjusting the Interpupillary Distance
5.6 Adjusting the Diopter
5.7 Focus
The interpupillar distance range:
48mm～75mm. When observing
with two eyes, hold on the left and
right prism holder, turn the axis,
adjust the interpupillary distance
until the left and right fields of view
coincide completely as shown in
figure 21.
The right ocular tube is fixed. So by
turning the left diopter ring after
the right ocular focus on the
specimen, the operator who’s left
and right eye has different eyesight
can obtain a comfortable focus
position with both eyes (see figure
22).
This microscope has automatic
focus function. Press the "auto-
f o c u s b u t t o n " g e n t l y , t h e
mi cro sc ope w il l f ocu s on t he
specimens automatically .
T h e " R T F s t a t u s i n d i c a t i o n "
showes the work state of the "Real-
time focus on the button". "RTF
ON" means features real-time
focus is working,the microscope
can do the auto-focus in real time
(See figure 23).
A f t e r t h e i n i t i a l i z a t i o n , t h e
specimen should be around in the
image plane.If you have lift the Z-
axis during the operation, please
do a 4 X focus again to esure the
microscope working normally.Do
the steps as follow: Press the
"objectives change button" gently
to switch the 4 × objective into the
center of the optical path, then
press the "Auto-focus button" to
focus on the specimen again .now
you can use the microscope to do
o b s e r v a t i o n w i h t a n y
magnification.

17
5. Adjustment Operation
Swing Condenser
A p e r t u r e
Diaphragm
Field Diaphragm
Optical Switch Push Rod
fig. 24
fig. 25
fig 26
5.8 Adjust the Swing Condenser
5.9 Adjusting the Field Diaphragm
5.10 Switch The Optical Path
The center of the condenser and the light
axes of the objective are coaxial. It has
been adjusted at the factory, so you needn’t
to adjust them by yourself.
The highest position of the condenser has
been adjusted too. It also needn’t any
user’s operation.
Turn the condenser focus knob to shift the
condenser. It needs to raise the condenser
w h e n u s i n g t h e h i g h m a g n i f i c a t i o n
objective, and to decline when using the low
magnification one.
1.Using the Swing Condenser
When using the low magnification objective,
turn out the condenser, and let it away from
th e lig h t pa t h. Wh ile u sin g the h igh
magnification objective, turn it into the light
path (Figure 24).
2.Adjusting the Aperture Diaphragm
The aperture diaphragm is designed for the
adjustment of the numerical aperture , not
for the brightness. Generally, reducing the
diaphragm opening to 70- 80% of the N.A.
val ue of the respective objecti ve will
provide an image of acceptable quality. If
you want to observe the image of the
aperture diaphragm, remove one eyepiece
and look through the tube. You will see a
dark circle encroaching on the bottom of the
tube(Figure 24).
The control for the field diaphragm is a ring
use d for adju s t i n g t h e a rea of fiel d
diaphragm. When using, turn the ring to
reduce the field diaphragm, look into the
field, if the diaphragm image is faintness,
do the follow steps: first, turn the condenser
focus knob, shift the condenser holder to
the position where the observed image of
the field of view is sharp; then open the field
diaphragm, let the image full of the field of
view , reduce the mixed light, improving
the quality of the image.
When the optical switch push rod of the
trinocular viewing unit is pushed in, all the
light enter the binocular tube, so you can do
the binocular observation. While the lever
pull out, some light still enter the binocular
tube, and the left go up , enter the video
tube, so you can observe the specimen with
the video equipmentes.

1.Moto 2.Sensor
3.LUM SET 4.Comm >>
Sensor Enabled：
X：=1 Y：=1 Z：=1 O：=1
Initial Luminance:
32
Comm BANDRATE:
19200
X Speed： >>
Startup：001 Max：100
5.Calibrate 6.AF SET
<<
Y Speed： >>
Startup：001 Max：100
Z Speed： >>
Startup：001 Max：128
18
6. Control Box Menu Setting
6.1 Main menu
6.2 Sub Menu
You can enter the Main Menu Setting by
pressing the OK key. And the display
panel will show as Fig.27. Use left and
right arrow to select the Menu. Press OK
key to enter Sub Menu.
1．Moto：stepper motor speed setting。
2．S e n s o r ：l i m i t s w i t c h
enabled/disabled.
3．LUM SET： Initial brightness setting.
4．Comm：communication baud rate
setting
5．Calibrate：system parameter setting
6．AF SET：Auto Focus reference
position setting
1．Moto(stepper motor speed setting)
Moto setting is used to set the stepper
motor’s start speed and maximum speed.
Use left and right arrow to select the
parameter. Use up and down arrow, or roll
the Z Axis Wheel to change the value.
Press OK key to save and exit the Moto
setting.
2．Sensor(limit switch enabled/disabled)
S e n s o r s e t t i n g i s u s e d t o
enabled/disabled the limit switch. When
the value set 1 which mean enabled the
limit switch, the microscope will drive
correspo n d i n g M O TO to the default
setting position after power on. When the
value set 0 which mean disabled the limit
switch, the corresponding MOTO do
nothing after power on. Use left and
rightarrow to select the parameter. Use up
and down arrow, or roll the Z Axis Wheel to
change the value. Press OK key to save
and exit the Sensor setting(see Figure
29).
3．LUM SET(Initial brightness setting)
LUM SET is used to set the startup
Luminance value. The value can be set
from 0 to 99. Use up and down arrow, or
roll the Z Axis Wheel to change the value.
Press OK key to save and exit the LUM
SET setting.
4．Comm(communication baud rate
setting)
C o m m s e t t i n g i s u s e d t o s e t t h e
communication baud rate. Recommend
value is 19200. Use up and down arrow, or
roll the Z Axis Wheel to change the value.
Press OK key to save and exit the Comm
setting.
fig. 27
fig. 29
fig. 30
fig. 31
fig. 28

X：Area Offset Init>>
75000 37500 37500
<Y：Area Offset Init>
55000 22500 22500
<<10--20 20--40 40--100
1600 1600 1600
<O：Init Offset 4--10>
4X 200 1600
<Z：Area Offset Init>
20000 9000 18000
4X-AF 10X-AF 20X-AF>
18000 18000 18000
<40X-AF 100X-AF
18000 18000
19
6. Control Box Menu Setting
6.2 Sub Menu
5．Calibrate(system parameter setting)
Calibrate setting is used to set the system
parameter.
Area set: is used to set the stepper moving
range.
Offset: is used to rectify the startup
position.
Init: is used to memorize the startup
position.
4—10, 10—20,20—40,40—100: is used
to set the stepper distance between
neighbor Objective. Use left and right
arrow to select the parameter. Use up and
down arrow, or roll the Z Axis Wheel to
change the value. Press OK key to save
and exit the Calibrate setting(see Figure
32).
6．AF SET(Auto Focus reference position
setting)
AF SET (Fig.10) is used to set the Auto
Focus reference position.This value is
useful to Auto Focus Module of Control
Box. With this data it can save a lot of time
to find focus. Use left and right
arrow to select the parameter. Use up and
down arrow, or roll the Z Axis Wheel to
change the value. Press OK key to save
and exit the AF SET. This parameter can
be set as follow. First, put a common slice
on the Stage. Then find the focus position
by controlling the Z Axis Wheel.Repeat
this process with all the objectives and
record the focus position’s value. After
that enter the AF SET menu and displace
the value of 4X-AF, 10X-AF, 20X-AF, 40X-
AF and 100X-AF by the new recorded
value.Finally enter the menu Calibrate
(system parameter calibration), adjust the
Offset of the Z axis , make the stage 's
initial position near the focal plane. Adjust
the Z axis'parameters ,make them same
as the 4X-AF initial parameters.If these
parameters are setted correctly, the stage
will be moved to the near position of
focus.That will obviously reduce the time
of auto focus and real-time focus ( Figure
33 ).
fig. 3 2
fig. 33