BestScope BS-2052 Series User manual

Biological Microscope
BS-2052 Series
Instruction Manual
This manual is for BS-2052 series biological microscope. To ensure the safety, obtain optimum
performance and to familiarize yourself fully with the use of this microscope, it is recommended strongly
that you study this manual thoroughly before operating the microscope.

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CONTENTS BS-2052
CONTENTS .........................................................................................................0
USER NOTICE......................................................................................................2
1. Components Name ........................................................................................3
2．Assembly......................................................................................................4
2.1 Assembly Diagram..................................................................................4
2.2 Assembly procedure...............................................................................5
3. Adjustment and Operation.............................................................................9
3.1 Adjustment set diagram .........................................................................9
3.2 Adjustment and Operation...................................................................11
4．Technical Specifications..............................................................................15
5．Outfit .........................................................................................................17
6．Troubleshooting Guide...............................................................................19

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USER NOTICE BS-2052
I. Safety Note
1. Open the box carefully to avoid the accessories, like lens, dropping to ground or being damaged.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and easy
shaking environment. Make sure the stage is flat, horizontal and firm enough.
3. When moving the microscope, carefully carry it with the handle and the base.
4. Make sure the instrument is earthed, to avoid lighting strike.
5. For safety, be sure the main switch is in “O” (off) state and cut off the power supply before
replacing the bulb or the fuse. If you replace the bulb during use or right after use, allow the
lamp bulb and the lamp house to cool completely before touching.
(Designated bulb: 1W S-LED or 6V20W Halogen Lamp)
6. Check the input voltage: be sure the input voltage which is signed in the back of the microscope
is consistent with the power supply voltage, or it will bring a serious damage to the instrument.
7. Always use the power cord provided by BestScope.
8. The electrical equipment of the microscope should be discard as electronic waste.
II. Maintenance and Care
1. All the lenses have been adjusted properly; do not dismount them by yourself please.
2. The nosepiece and coarse and fine focusing parts are so delicate that it is forbidden to
disassemble them carelessly by yourself.
3. Keep the instrument clean, and do not pollute the optical element when wiping away the dust
on the instrument.
4. The contaminations on the prism, like fingerprints and oil smudges, could be gently wiped with
a piece of soft cloth or tissue paper, gauze which has been immersed in pure alcohol or ether.
(Note that the alcohol and ether are highly flammable, do keep them away from the fire or
potential sources of electrical sparks, and use them in a drafty room as possible as you can.)
5. Do not attempt to use organic solvents to clean the microscope components other than the
glass components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted
neutral detergent.
6. When using, if the microscope is splashed by liquid, cut off the power at once, and wipe away
the splash.
7. Do not disassemble any parts of the microscope, as this will affect the function or reduce the
performance of the microscope.
8. Place the instrument in a cool, dry position. When not using the microscope, keep it covered
with a dust cover. Make sure the lamp socket is cool before covering the microscope.

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1. Components Name BS-2052
Eyepiece
Binocular viewing head
Nosepiece
Objective
Main body
Stage
Condenser
Illumination

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2．Assembly BS-2052
2.1 Assembly Diagram
The following figure shows the installation sequence of the components. The number in the figure
shows the assembly steps.
Before installing, be sure every component is clean, do not score any parts or glass surface.
Keep well with hexagon wrench provided. When changing the components, you will need it again.
Main body
Objective
Binocular viewing head
10X Eyepiece
Power cord
①
②
③
④

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2.2 Assembly procedure
Fig.1
Fig.3
Fig.2
2.2.1 Installing binocular viewing head
(Fig.1-2)
Insert the binocular viewing head into the
microscope head and turn it to a proper
position, then tight it.
2.2.2 Installing the eyepiece (Fig3-4)
Insert the eyepiece into the eyepiece
tube until they are against each other, as
shown in Fig.4.
Fig.4
4

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Fig.6
Fig.5
2.2.3 Installing the objective (Fig.5-6)
1. Adjusting the coarse focus knob until the
support device of the mechanical stage
reaches its low limit position.
2. Screw the lowest magnification objective
into the nosepiece from the left or the right
side, then revolve the nosepiece clockwise
and mount other objectives by the
sequence of low to high magnification
Installing objective this way will make the
change of magnification to be easier during
using.
Clean the objective regularly, for lens is
susceptible to dust.
When operating, use 10×magnification
objective to search and focus specimen
firstly, then replace with higher
magnification objective if necessary.
When replacing the objective, slowly turn
the nosepiece until you hear “clicked”,
that means the objective is in place.
2.2.4 Mounting the filters (Fig.7)
1. Open the condenser carriage①;
2. Place the required filter②into the
condenser, then close the condenser
carriage.
★the filter of the standard outfit is green
and baby blue.
Fig.7

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2.2.4 Connecting the Power Cord
(Fig.8-10)
★The cable and cords are vulnerable when
bent or twisted, never subject the power cord
to excessive force.
1. Set the main switch①to “O” (off) state
before connecting the power cord.
2. Insert the plugs②into the power jack③
of the microscope safely.
3. Plug the power cord④into the power
supply receptacle⑤. Make sure the
connection is well.
★Do use the supplied power cord all the
time. If lost or damaged, select the same
standard cord, please.
★A wide range of voltage, 100V～240V, is
acceptable for this microscope.
2.2.5 Replacing the fuse (Fig.9-10)
Do remember to set the main switch①to
the state “O” (OFF) and unplug the power cord
before replacing the fuse. Rotate the fuse out
of the holder③, replace with a new fuse, then
rotate it back to the holder again.
Fig.9
⑤
Fig.10
Fig.8
④
③
①
②

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2.2.8 Installing and Replacing the bulb
(Fig.11-12)
Please use the specified halogen Lamp 6V20W.
1. Loosing the screw①.
2. Hold the bulb②after you wrap it with gauze
or other protection materials, and then insert
its pin deeply into the jack in the lamp holder.
3. During microscope using or soon after it is
turned off, the bulb, the lamp house and
nearby parts will be very hot and will cause
serious burns. Please turn the main switch to
“O”(off) and disconnect the power plug, and
make sure the bulb, the lamp room and
periphery are all cool. Then, you can do your
replacing.
Insert the bulb gently. Squeezing too hard will
damage the bulb.
To prevent reduced bulb life or cracking, do not
touch the bulb with bare hands. If fingerprints
are accidentally left on the bulb, wipe the bulb
with a soft cloth.
Fig.11
Fig.12
①
②

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3. Adjustment and Operation BS-2052
3.1 Adjustment set diagram
Right Fine Focus Knob
Right Coarse Focus Knob
Tension Adjustment Collar
Main Switch
Portrait Adjustment Knob
Lateral Adjustment Knob
Fig.13
Aperture Iris Diaphragm adjustment

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BS-2052
Left Coarse Focus
Knob
Interpupillary Distance Indicator
Brightness Adjustment Knob
Fig.14
Clocking Set
Condenser Focusing Knob
Left Fine Focus Knob

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3.2 Adjustment and Operation
3.2.1 Brightness Adjustment (Fig.15-16)
1. Connect the power cord and set the main
switch①to “－”state (ON).
2. Turning the brightness adjustment knob ②.
3.2.2 Placing the Specimen (Fig.17)
1. Place the specimen the center of the mechanical
stage and use the stage clips to clamp it.
2. Turn the portrait and lateral adjustment
knob①of the mechanical ruler, move the
specimen to the required position.
Be careful when changing the objective. If you
finish the observation with the short working
distance objective, and want to change another one,
be careful of not letting the objective touch the
specimen.
3.2.3 Focusing the Specimen (Fig.18)
1.Focus the specimen with 10X objective. To avoid
the objective touching the specimen during focusing,
you should raise the mechanical stage to let the
specimen close to the objective at first, then slowly
separate them to bring the specimen to focus.
Turn the coarse focus knob①conversely to
lower the specimen and search images in the
10×ocular simultaneously, and then use the fine
knob②to focus. After that, you can replace with
other magnification objectives safely, and focus
without the risk of damaging the specimen.
To make the observation more convenient, you
can use the locking set③to fix the stage in a
vertical direction.
Fig.15
Fig.18
Fig.17
①
①
②
①
③
Fig.16
②

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3.2.4 Condenser Adjustment (Fig.19)
Turn the condenser focus knob①to move the
condenser up and down. Raise the condenser when
using the high magnification objective, and descend
it when using the low magnification one.
1. Focus the specimen with 10× objective.
2. Adjust the condenser focus knob①to get a
clear image of the field iris diaphragm.
3. Turn the condenser centering knobs②to
center the image of field iris diaphragm in the
field of view.
The condenser and the objective are coaxial. It
has been adjusted before leaving factory, so please
do not adjust them by yourself.
The highest position of the condenser has been
adjusted too. It also needn’t any user’s operation.
(The top surface of the condenser is
0.03mm-0.4mm lower than the stage top surface.)
3.2.5 Aperture Iris Diaphragm Adjustment
(Fig.20)
Turn the aperture iris diaphragm stick①to
adjust the aperture iris diaphragm.
The aperture iris diaphragm is designed for
the adjustment of the numerical aperture, not for
the brightness.
Generally, setting the aperture iris diaphragm
to 70- 80% of the N.A. of the objective in use will
provide an image with good contrast. If you want
to observe the image of the aperture iris
diaphragm, remove one eyepiece and look through
the tube. You will see a dark circle encroaching on
the bottom of the tube.
图20
Fig. 19
Fig.20
②
①
①

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3.2.6 Adjusting the Interpupillary Distance
(Fig.21)
The interpupillary distance range: 47mm～
78mm.
While looking through the eyepieces, move
both eyepieces round until the left and right fields
of view coincide completely.
3.2.7 Adjusting the Diopter (Fig.22)
1. Turn the eyepiece①to adjust the diopter
while looking through it.
★ The diopter range of the eyepiece is ±5
diopter. The number aligned to the line on
the viewing head is the diopter in use.
3.2.8 Adjusting the tension adjustment
collar (Fig.23)
Turn the tension adjustment collar①with
your fingers. When the collar is turned in the
direction of the arrow, the tension of the coarse
adjustment knob②increases. Turning the collar in
the opposite direction decreases the tension.
If the nosepiece descends on its own or if the
specimen gets out of focus quickly even when it is
brought into focus using the fine adjustment
knob③, it means the tension of the coarse
adjustment knob is too low. Turn the collar in the
direction of the arrow to increase the tension.
Fig.22
Fig.23
Fig.21
①
①
②
③

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3.2.9 Adjusting the Interpupillary Distance
(Fig.24)
The microscope with ECO:
The equipment can induct the front
surroundings in 1m. At the moment, it light red and
light source keeping working.
When people left and no object within the front
1m, the red light would flashed. After 15 minutes,
light source stop working.
Fig.24
ECO

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4. Technical Specifications BS-2052
4.1 Main Specifications
Optical System
Finite optical system / Infinite optical system
Viewing Head
Seidentopf Binocular Head, 30°Inclined, Interpupillary Distance 47-78 mm
Eyepiece
WF10X/18 or WF10X/20
Nosepiece
Backward Quadruple Nosepiece
Objective
Achromatic Objective / Infinite Semi-plan Objective 4×, 10×, 40×, 100×
Focusing
Coaxial Coarse and Fine Adjustment, Fine Division 0.002mm, Moving Range 20mm
Condenser
Abbe Condenser, NA1.25
Stage
Double Layers Mechanical Stage160×140mm, Moving Range 74×50mm
Rackless Timing Belt Stage150×139mm, Moving Range 75×52mm
Illumination
1W S-LED /6V20W Halogen Lamp
4.2 Eyepiece and objectives
4.2.1 Achromatic Objective
Magnification
Numerical
Aperture (NA)
Focal Length (mm)
Focal distance (mm)
Working Length
(mm)
Objective
4×
0.10
0.17
31.05
37.5
dry
10×
0.25
0.17
17.13
7.316
dry
40×
0.65
0.17
4.65
0.632
dry
100×
1.25
0.17
2.906
0.198
oil
4.2.2 Infinite Semi-plan Objective
Magnification
Numerical
Aperture (NA)
Focal Length (mm)
Focal distance(mm)
Working Length
(mm)
Objective
4×
0.10
0.17
45
16.8
dry
10×
0.25
0.17
18
5.8
dry
40×
0.65
0.17
4.5
0.43
dry
100×
1.25
0.17
1.8
0.13
oil

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4.2.3 Eyepiece
Eyepiece
Magnification
Focal Length (mm)
Field of View (mm)
Wide field eyepiece
10×
24.95
Φ18
Wide field eyepiece
10×
25
Φ20
4.2.4 Total Magnification
Eyepiece
10×
10×
10×
10×
Objective
4×
10×
40×
100×
Total Magnification
40×
100×
400×
1000×

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5. Outfit BS-2052
Note: “●”Standard Outfit, ○”optional
Item
Specification
Quantity
Standard Outfit
BS-2052A/AT
Main Body
Main Stand
1
●
Battery Charging
1
○
ECO Function
1
○
Double Layers Mechanical Stage
1
●
Condenser Holder
1
●
Viewing Head
Seidentopf Binocular Head
1
●
Seidentopf Trinocular Head
1
○
Condenser
Abbe Condenser, NA=1.25
1
●
Nosepiece
Backward Quadruple Nosepiece
1
●
Illumination
1W SLED
1
●
6V20W Halogen Lamp
1
○
Blue, Green Filter
1 each
○
Spare Fuse 50T 250V 500mA
2
●
Eyepiece
10×/18 Wide Field Plan Eyepiece
2
●
Objective
Achromatic Objective4×
1
●
Achromatic Objective10×
1
●
Achromatic Objective40×
1
●
Achromatic Objective100× (Oil, Spring)
1
●

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Note: “●”Standard Outfit, ○”optional
Item
Specification
Quantity
Standard Outfit
BS-2052B/BT
Main Body
Main Standard
1
●
Battery Charging
1
○
ECO Function
1
○
Rackless Stage
1
●
Condenser Holder
1
●
Viewing Head
Infinite Seidentopf Binocular Head
1
●
Infinite Seidentopf Trinocular Head
1
○
Condenser
Abbe Condenser, NA=1.25
1
●
Nosepiece
Backward Quadruple Nosepiece
1
●
Illumination
1W SLED
1
●
6V20W Halogen Lamp
1
○
Blue, Green Filter
1 each
○
Spare Fuse 50T250V 500mA
2
●
Eyepiece
10×/20 Wide Field Plan Eyepiece
2
●
Objective
Infinite Semi-plan Objective 4×
1
●
Infinite Semi-plan Objective 10×
1
●
Infinite Semi-plan Objective 40×
1
●
Infinite Semi-plan Objective 100× (Oil,
Spring)
1
●

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6．Troubleshooting Guide BS-2052
6.1 Optical System
TROUBLE
CAUSE
SOLUTION
1. The edge of the field of
view is dark or the
brightness is not uniform
The nosepiece is not in the located
position (objective and light path not
coaxial)
Locate the nosepiece
properly where it clicks
The surface of the lamp becomes black
Change a new lamp bulb
A lens (the objective, condenser,
eyepiece or collector) is dirty.
Clean it thoroughly
2. Dirt or dust is visible in
the field of view
Dirt/dust on the specimen
Replace with a clean
specimen
Dirt/dust on the eyepieces
Clean them
3. Visibility is poor
Image is not sharp;
Contrast is poor;
Details are indistinct
Specimen is not covered
Add cover glass on it
The thickness of the cover glass is not
suitable
Use standard cover glass
with thickness of 0.17mm
Specimen is placed reversely
Turn it over
Dry objective has oil on it. (especially for
40X objectives)
Wipe the oil
A lens (the objective, condenser,
eyepiece or collector) is dirty.
Clean it
Immersion oil is not used with the 100x
objective
Use specified oil
Air bubbles existed in the immersion oil
Eliminate the bubble
The aperture iris diaphragm is stopped
down too far
Adjust the aperture iris
diaphragm properly
Dirt or dust on the eyepiece
Clean it
4. One side of image is
blurred
Condenser is not properly centered
Center the condenser with
the centering screw
The nosepiece is not properly engaged
Engage the nosepiece
properly
The specimen is not clamped
Clamp it with stage clips
5. The brightness is not
enough
The aperture iris diaphragm is too small
Adjust it properly
The condenser is too low
Adjust it properly
A lens (the objective, condenser,
eyepiece or collector) is dirty.
Clean it