biochrom Ultrospec 50 User manual

Biochrom Ultrospec 50, 60, 70 & 80
UV Visible Spectrophotometers
Accessories User Manual

TABLE OF CONTENTS:
1. HEALTH & SAFETY ...........................................................................................................................1
2. OPERATION .....................................................................................................................................1
3. INSTALLATION.................................................................................................................................1
4. PASSIVE ACCESSORIES.....................................................................................................................2
4.1. SINGLE CELL HOLDER ..............................................................................................................3
4.2. VARIABLE PATHLENGTH CELL HOLDER ...................................................................................4
4.3. WATER THERMOSTATED CELL HOLDER ..................................................................................4
4.4. FILM HOLDER ..........................................................................................................................4
4.5. TEST TUBE HOLDER.................................................................................................................5
4.6. PELTIER CONTROLLED FLUID CIRCULATOR.............................................................................6
5. ACTIVE ACCESSORIES ....................................................................................................................18
5.1. 8-POSITION CELL CHANGER ..................................................................................................19
5.2. 5-POSITION THERMOSTATTED CELL CHANGER ....................................................................19
5.3. SIPPER & FLOWCELL..............................................................................................................20
6. INSTALLING THE INTERNAL PRINTER ............................................................................................24

Issue 02 1
1. HEALTH & SAFETY
2. OPERATION
The operation of all Biochrom Ultrospec accessories is described in the relevant user manual.
3. INSTALLATION
Before installing any accessory in the Biochrom Ultrospec you must ensure that the instrument is
disconnected from the mains.
Note: The images in this manual show the installation of accessories in a double beam instrument.
Accessories are installed in split beam instruments in exactly the same way. All accessories detailed
in this manual are available for both split beam (Ultrospec 50) and double beam instruments
(Ultrospec 60, 70 & 80).
Care must be taken when handling all heated accessories
There are no bio-hazardous materials within the unit; however this unit could be used with
bio-hazardous samples. Before using the instrument the customer should have in place
decontamination procedures designed to protect laboratory workers from occupationally acquired
infections. The sample chamber cell holders are removable and may be decontaminated using the
appropriate disinfectant for the bio hazard in question, rinsed with distilled water and then allowed
to dry. The sample chamber and exterior may be wiped with a suitable disinfectant cleaning wipe.
Decontamination. Equipment returned for repair should include an appropriate
decontamination certificate
It is the responsibility of the customer to ensure that the user of the equipment is provided
with a safe working environment.
Any chemicals used in Analyses should be used, stored and disposed of in accordance with
manufacturer’s guidelines and local safety regulations
Toxic Fumes. Efficient laboratory ventilation must be provided when working with volatile
solvents or toxic substances
Waste disposal. Disposal of some solvents and chemicals may be classed as hazardous waste
and must be dealt with in accordance with local regulatory practice.
Personal protective equipment. This is not required to operate the unit but the samples measured
may require PPE. A local risk assessment should be carried out.
Toxic fumes.

Issue 02 2
4. PASSIVE ACCESSORIES
Accessories that do not interact with the instrument’s software are named passive accessories.
There installation is described below:
To allow easy access to the thumbscrew at the front of the accessory open the cell chamber lid and
lift out the front panel (below left).
With the front panel removed the accessory is removed and replaced as outlined below:
1. Loosen the front thumb screw by turning anticlockwise.

Issue 02 3
2. With the thumb screw fully unscrewed, lift the accessory
and gently pull forward.
3. To insert the desired accessory, lift the front end, slide into
the brackets at the back of the cell chamber (indicated by the
two arrows) and tighten the thumb screw by turning
clockwise.
With the accessory inserted simply replace front panel, switch on the instrument and it will be ready
to use.
4.1. SINGLE CELL HOLDER
The single cell holder is designed to take
measurements of 10 mm pathlength cuvettes. All
Biochrom Ultrospec are supplied with a single cell
holder as standard.

Issue 02 4
4.2. VARIABLE PATHLENGTH CELL HOLDER
The variable pathlength cell holder is designed to use 1,
2, 5, 10, 20, 50 & 100 mm pathlength cells. To
accommodate different pathlengths the clips should be
moved to secure the cell and spacers used as required.
4.3. WATER THERMOSTATED CELL HOLDER
The water thermostated cell holder is designed to
work with water circulators to achieve constant
temperatures in the range 5 to 80 °C. The accessory
has to be connected to an external fluid circulator so
that the water flows into the bottom of the cell
holder (the right hand inlet) and flows out through
the top of the cell holder (the left hand outlet).
4.4. FILM HOLDER
The film holder is designed to measure the
absorbance of solid materials including thin flexible
sheet materials such as plastic, acrylic, gelatine.
Applications also include the quality control of
tinted optical lenses.

Issue 02 5
4.5. TEST TUBE HOLDER
The test tube holder allows samples to be measured
in a test tube of between 10 and 24mm diameter
and up to 160mm tall

Issue 02 6
4.6. PELTIER CONTROLLED FLUID CIRCULATOR
Content
4.6.1. GENERAL INFORMATION ................................................................................................7
4.6.1.1. General Warnings ...................................................................................................7
4.6.1.2. Quality, Reliability and Safety .................................................................................7
4.6.1.3. SAFETY MARKINGS..................................................................................................8
4.6.2. INSTRUMENT DESCRIPTION............................................................................................8
4.6.2.1. Controller front and rear panel...............................................................................9
4.6.2.2. Specifications ..........................................................................................................9
4.6.2.3. User interface........................................................................................................11
4.6.3. INSTALLATION...............................................................................................................12
4.6.3.1. Checking the serial number ..................................................................................12
4.6.3.2. Checking mains supply and fuses..........................................................................12
4.6.3.3. Instrument set-up .................................................................................................13
4.6.4. OPERATING INSTRUCTIONS ..........................................................................................14
4.6.4.1. Manual operation .................................................................................................14
4.6.5. MAINTENANCE..............................................................................................................16
4.6.5.1. Cleaning the instrument .......................................................................................16
4.6.5.2. Water level alarm..................................................................................................16
4.6.6. TROUBLESHOOTING......................................................................................................17
4.6.6.1. Audible alarm........................................................................................................17

Issue 02 7
4.6.1. GENERAL INFORMATION
4.6.1.1. General Warnings
Check the voltage selector
Always disconnect the mains plug before starting any work inside the instrument
Set the temperature to the
required values on power on
If using substances dangerous for health and
the environment, observe the laws and
the standards in force in the laboratory where this instrument is used
If using flammable or explosive substances take the necessary precautions
4.6.1.2. Quality, Reliability and Safety
This equipment has been designed with emphasis on QUALITY, RELIABILITY and SAFETY, and we will
accept responsibility for these aspects only if the following conditions are met:
Electrical installation of the room or building in which the equipment is to be used must
comply with regulations specified in the country where the equipment is to be used.
The equipment must be used in accordance with the instructions for use provided by
BIOCHROM.
All modifications and repairs to the equipment must be carried out by BIOCHROM or its
authorised Agents.
Modifications must not be carried out unless they conform to approved Engineering Service
Information issued according to the appropriate BIOCHROM procedure.
The equipment installation must be carried out in accordance with local requirements for
responsibility and warranty.

Issue 02 8
4.6.1.3. Safety Markings
CE mark
The "CE" mark for this product makes reference to the directives
•89/336/EEC amended by 93/68/EEC for electromagnetic compatibility
•73123/EEC amended by 93/68/EEC for low voltage
COPYRIGHT All rights reserved. The information contained in this publication may not be used
for any purpose other than that for which it was originally supplied. The publication may not be
reproduced in part or in whole without written consent of BIOCHROM. In order to maintain and
improve standards of manufacturing, methods of functioning and to increase reliability, BIOCHROM
equipment is periodically reviewed. For this reason, the contents of this publication are subject to
change without notice.
4.6.2. INSTRUMENT DESCRIPTION
The Controller is a Peltier effect thermostatting system using an internal sealed liquid circuit allowing
full compatibility and connection to all thermostattable accessories.
The Peltier effect is based on the reversed thermoelectric effect. This is a phenomenon in which a
temperature difference is caused by a flow of current through a closed circuit consisting of two
different metals.
The Controller provides temperatures ranging from 20 °C to 60 °C with ±1 °C stability and ±0.1 °C
repeatability. The features of the Controller include:
Peltier effect for heating and cooling the liquid of circulation
Internal flow pump to circulate the liquid
The Controller can operate in either manual or remote control via a USB port
The Controller is supplied with one set of thermally insulated connection tubes to connect
the Controller to any thermostattable accessory
The Controller is supplied with a water level sensor to ensure correct operation
The Controller is supplied with high temperature sensor to avoid damaging the system and
malfunctions

Issue 02 9
4.6.2.1. Controller front and rear panel
The front and the rear panel have grids to dissipate the heat generated in the unit. Two fans force
the air movement. These grids must be free to guarantee the instrument specifications.
The front panel features the display with keypad.
The rear panel features the mains power connection with mains voltage selector, the USB connector
for remote control, the mains switch and the "Water Fill" inlet and outlet connections.
4.6.2.2. Specifications
4.6.2.2.1. Keypad/Display Unit
The keypad/display unit has a LCD screen. All programmable parameters can be displayed in specific
menus.
Three buttons are used to access the various functions and change the set parameters.
4.6.2.2.2. Programmable Parameters
Temperature
4.6.2.2.3. Displayed Values
the current thermostat temperature
the set temperature
the heating/cooling power
4.6.2.2.4. Operating Specifications
Temperature range from 20°C to 60°C
Temperature accuracy better than ± 0.15 °C from 20 °C to 60 °C
Temperature repeatability typically ± l °C
Temperature stability typically ± 0.1 °C
Specifications valid for ambient temperatures from 15°C to 35°C

Issue 02 10
4.6.2.2.5. External Control Possibilities
USB as standard.
4.6.2.2.6. Dimensions
Width: 170 mm Height: 280 mm Depth: 430 mm Weight: 6 kg
4.6.2.2.7. Electrical data
Mains supply: 115/120 V or 220/240 V t 10% (factory setting 220/240 V)
Frequency: 50/60 Hz
Power: 120 VA
CE marked

Issue 02 11
4.6.2.2.8. Description of Symbols
Caution (Refer to accompanying documents).
Symbol identifying the use of substances dangerous for health and environment. For
these substances reference must be made to the laws and standards in force in the
laboratory where this instrument is used. If using flammable or explosive substances
take the necessary precautions.
Note: Connection to the protective ground is ensured through the power supply cable which has
to be in accordance with CEE 7 (IEC 23-5).
4.6.2.2.9. Environmental conditions
Performance and safety of the Controller are guaranteed at all times if it is operated under the
following environmental conditions:
Installation category II with smaller transient over voltages (standard IEC 664)
Pollution degree 2 (standard IEC EN 61010-1)
The instrument is designed for internal use and altitudes below 2000 m
Mains voltage fluctuations must not exceed t10% of the nominal voltage
In locations where the relative humidity is lower than 80%
In locations free from dust or vapours of solvents and acids
Room temperature between 10°C and 35°C
4.6.2.3. User interface
The menu-driven interface of the Controller allows the user to set the temperature and to view the
configuration.
Three buttons are used to access the various functions and change the set parameters.
The multi-functional Controller with its display is described below under manual operation.

Issue 02 12
4.6.3. INSTALLATION
4.6.3.1. Checking the serial number
The plate containing the serial number is located on the rear panel at the base of the unit. Check
that this number is identical to the serial number on the delivery note. If not, please inform
BIOCHROM immediately.
4.6.3.2. Checking mains supply and fuses
The Controller is factory set to operate at 220 VAC.
For operation at a voltage of 120 VAC, the voltage selector at the rear of the Controller unit must be
reset as described below.
Power supply
Type of fuse - 250 V
240 V
T 1A, slow-blow
120 V
T 2A, slow-blow
Adjustment for frequency change between 50 and 60 Hz is not necessary.
Fig. 3-1: Selecting the voltage
Voltage selector with fuse
Main switch

Issue 02 13
4.6.3.2.1. Changing the Mains Voltage Setting and Fuses
The voltage set is shown in the window of the voltage selector below the mains input connection. To
change this setting switch off the unit at the mains switch, remove the mains cable and proceed as
follows:
Slide out the fuse holder by inserting a small screwdriver alternatively into the two slots at the left
and right and pulling it out of the housing.
Remove the original fuses and replace with those of correct rating (see above) and push the holder
back in position.
Move the slider of the voltage selector with a screwdriver to the desired value. The new selected
voltage will be now visible.
4.6.3.3. Instrument set-up
4.6.3.3.1. Start up
The Controller is supplied completely assembled. It is only necessary to make the appropriate
connections.
Before starting the Controller you should familiarize yourself with the key functions as described in
section 4.
Position the Controller at a suitable position in your system
Leave at least 5 cm in front and the back of the Controller for free air circulation.
Unscrew the tap on the "Vent" connector.
Mount the "Vent" connector supplied with the instrument.
Connect the supplied tubes to "Water in" and "Water out" fast connections.
Connect the tubes to the accessory to be thermostatted.
Switch on the Controller.
The instrument initializes and after one minute it is ready to go.
Check the system for water level ("Water level alarm sensor).

Issue 02 14
4.6.3.3.2. External Connections
The Controller has no special electrical external connections which would allow relay control etc.
Operations are performed either manually or via remote control using the USB connections.
Contact BIOCHROM for further information about the remote control functions.
4.6.4. OPERATING INSTRUCTIONS
4.6.4.1. Manual operation
Main page and functions of the buttons
Page
Display
L button
Middle
button
R button
Stand by
Stand by
On
Main
Normal status
Current temperature
Set temperature
Heating / cooling power
(R)
Menu
Off
Modify the
temperatur
e setpoint
Temperature
setpoint
Flashing selected
parameter
Down
Up
Change
Alarm status
Alarm description
Menu
Beep on /
off
Reset
alarm /
Change (1)
Menu
Menu selection
Scroll
Select
Exit
(1) Only if the manual alarm reset option has been configured

Issue 02 15
Menu page and functions of the buttons
Menu
Description
L button
Middle
button
R button
Parameter
User parameters
Scroll
Select
Exit
Configure
Configuration
parameters
Scroll
Select
Exit
Diagnostic
Diagnostic values
Scroll
Select
Exit
Parameters setting
User parameters and functions of the buttons
Number
Description
Default
value
L button
Middle
button
R button
P-01
Temperature unit of measure
°C
Scroll
Select
Change
P-02
Auto start (after switching on)
NO
Scroll
Select
Change
P-03
Contrast
5
Scroll
Select
Change
P-04
Beep on/off
YES
Scroll
Select
Change
P-05
Manual alarm reset
NO
Scroll
Select
Change
Configure parameters
A password is required to enter this menu. CAUTION: all these parameters are correctly set in the
factory: no changes should be made by the user!!!

Issue 02 16
Diagnostic parameters
Description
Unit of measure
Cell temperature
°C
Radiator temperature
°C
Water resistance
Kohm
Power
%
Cell voltage
Volt
4.6.5. MAINTENANCE
The Controller requires very little routine maintenance by the user.
Any operation that requires the units to be opened must only be performed by BIOCHROM
technicians or authorized personnel.
Always unplug from the mains when opening the units
4.6.5.1. Cleaning the instrument
For normal cleaning operations on the instrument only use a cloth dipped in water or neutral
detergent, do not use organic or abrasive solvents.
4.6.5.2. Water level alarm
Check for signs of water level. It is recommended that this is done prior to starting analysis. If a
"Water level" alarm is signalled immediately check for spillage in the accessory compartment and
remove it with appropriate measures. Using the refilling bottle supplied to fill the system with liquid
via the inlet or outlet connectors until the light turns off.

Issue 02 17
4.6.6. TROUBLESHOOTING
The Controller is able to indicate malfunctions and incorrect use. The messages available are directly
visible on the display, indicated audibly or transmitted via USB for remote communication.
4.6.6.1. Audible alarm
The Controller will send an audible alarm if either a leakage was detected or another failure occurs.
4.6.6.1.1. Water level Alarm
The Controller is equipped with a "Water level" sensor. This means even small leakage will be
detected.
After the leakage is signalled on the Controller press the RESET ALARM button and remove the
leakage immediately by checking all connections and fill up the system with distilled water.
4.6.6.1.2. Other system Alarms
The following alarms are available:
Cell temperature too high
Radiator temperature too high
Cell temperature sensor failure
EEPROM memory failure
If one of these alarms is activated, the Controller will signal the failure by an audible signal and
display relative messages on the display.
Action: Switch off the instrument immediately!
If the failure does not disappear after the instrument is switched on again, contact service.
IMPORTANT!
THE CONTROLLER IS SHIPPED ALREADY FILLED WITH DISTILLED WATER. DURING
INSTALLATION, WHEN CONNECTING THE TUBING THE WATER LEVEL IN THE TANK WILL
DROP AND WILL NEED TO BE TOPPED UP. ONLY USE THE DISTILLED WATER SUPPLIED IN
THE SEPARATE 200CC CONTAINER INSIDE THE PACKAGING TO TOP UP THE WATER IN
THE UNIT.

Issue 02 18
5. ACTIVE ACCESSORIES
Accessories that interact with the instrument’s software are named active accessories. The
operation of active accessories is described in the relevant user manual.
The installation of active accessories that use the internal accessory connector is outlined below.
Note: The Biochrom Ultrospec will be supplied with a single cell holder as standard; this is removed
as described above.
1. To insert the accessory, lift the front end, slide into the
brackets at the back of the cell chamber (indicated by the
two arrows) and tighten the thumb screw by turning
clockwise.
2. With the accessory inserted and secured using the
thumb screw, plug the 15-pin plug into the internal
accessory connector.
3. With the accessory inserted and plugged into the internal
accessory connector, replace front panel and switch on
the instrument. The accessory will be calibrated during
instrument start-up.