Bioer LifePro User manual

Caution: The Bioer Co. reserves the right to modify this manual at any time without notice.
The manual contains copyright protected and patented material. Without prior written consent from
Hangzhou Bioer Technology Co., Ltd., any part of the manual shall not be duplicated, reproduced or
translated into any other language.
File No.: BYQ60630000000ESM
File version: July 10, 2009 Version 1.0
Before initial use of this instrument, please carefully
read this manual
!

LifeProThermalCycler ImportantNotes
—i —
Important Notes
1. Usual practice
Caution: Important information is contained in any item and should be carefully read. Failure
to observe the instruction would result in damage or abnormal function of
instrument.
Warning! The warning message requires extremely careful operation of a certain step. Failure
to observe the instruction would result in serious personal injury.
2. Safety
During operation, maintenance and repair of this instrument, the following basic safety notes shall
be observed. In case of failure to follow these measures or the warnings or notes indicated herein,
the basic protection provided by the instrument, its safety criteria of design and manufacture, and its
predicted use range would be impaired.
Hangzhou Bioer Technology Co., Ltd. shall be irresponsible for the consequence resulted from
user’s failure to observe the following requirements.
Note: This instrument conforms to Class Ⅰand Protection Class IP20 of GB4793.1 Standard
and it is for indoor use.
a) Instrument earth
In order to avoid electrical shock accident, the input power wire of instrument shall be reliably
earthed. This instrument uses 3-core earth plug and this plug is provided with a third (earth) leg, is
merely used for earth type power socket and is a safety unit. If the plug is impossible to insert into
the socket, the socket shall be correctly fixed by electrician; otherwise, the earth plug would lose
safe protection function.
b) Keeping apart from the live circuit
The operator shall not disassemble instrument without permission. Only qualified professionals are
allowed to replace elements or adjust the machine inside. Replacement of elements under
energizing state shall be prohibited.
c) Use of power supply
Before turning on AC power, make the power voltage consistent with the instrument-required one (a
±10% deviation is allowed). Make the rated load for power socket not below instrument’s maximum
load.
d) Power wire
Generally, this instrument uses accompanied power wire. The damaged power wire shall be
replaced by one of the same type and the same specification. It can’t be repaired. The power wire
shall neither be compressed nor be laid in gathered personnel location.
e) Insertion and withdrawal of power wire
At insertion and withdrawal of power wire, the plug shall be correctly held with hand. At insertion, the
plug shall be completely and tightly inserted into the socket and at withdrawal heavily pulling the
wire shall not be allowed.
f) Placement of instrument
This instrument shall be fixed in a low RH and low dust place apart from water source (e.g. water
pool and water pipeline) and the room shall be well ventilated, and free of corrosive gas or and
interference of strong magnetic field. The instrument shall not be placed in a wet or dusty location.
The openings on this instrument are for ventilation circulation and in order to avoid over-heat of the

LifeProThermalCycler ImportantNotes
—ii —
instrument body they shall not be blocked or covered. When a single set or several sets of
instrument are used, the interval between its surrounding ventilation opening and its nearest object
shall not be below 50cm or 100cm. Also, don’t use the instrument on loose or soft surface, or the air
inlet at instrument bottom might be blocked.
Excessive temperature would impair the test performance or result in fault of instrument. This
instrument shall not be used in location subjected to direct sunshine. The instrument shall be kept
away from hot gas, stove and all other heat source.
If the instrument is to be set aside for a long time, the power plug shall be withdrawn and the
instrument covered with soft cloth or plastic film to avoid entrance of dust.
g) Notes during operation
During test, cares shall be taken to prevent liquid from dropping onto the instrument.
Caution: In any of the following cases, immediately cut off the power supply, withdraw the power
plug from the power socket, contact the supplier or ask qualified repair personnel for
treatment:
yLiquid drops into inside of the instrument.
yThe instrument is rained or watered.
yThe instrument works abnormally, especially generates abnormal sound or odor.
yThe instrument is dropped or its casing is damaged.
yThe function of instrument obviously changes.
3. Instrument label
Warning! The instrument is adhered with mark “HOT SURFACE!” During operation of instrument
or a period immediately after completion of running of a program, any portion of body
shall not be contacted with metallic portion (module or hot-lid) nearby the said mark;
otherwise skin would be burnt.
4. Maintenance of instrument
In order to ensure full contact and good heat conduction between the test tube and the conical holes,
the conical hole on the module shall be regularly cleaned with clean soft cloth soaked with a little
absolute alcohol. The stained surface of this instrument, if any, shall be cleaned with soft cloth
soaked with cleaning paste.
Warning! At cleaning of instrument, its power supply shall be cut off.
The instrument surface shall not be cleaned with corrosive cleaning agent.
5. After-sales service
The warranty content and scope are shown in the warranty sheet.
Note: yAfter unpacking, immediately check the goods against the packing list. In case of
damaged or missed goods, please immediately contact the supplier.
yAfter qualification of acceptance, properly fill the product acceptance sheet and send
(or fax) the copied sheet to the supplier for filing and maintenance.
yAfter unpacking, the packing box and packing materials shall be well kept for future
repair. For the damage of instrument occurring during transporting to the repair
department due to improper package, Hangzhou Bioer Technology Co., Ltd. shall bear
no liability.

User’s Manual of LifePro Thermal Cycler Content
CONTENT
Chapter I Introduction......................................................................................................................1
1. Introduction of PCR technology...................................................................................................... 1
2. Example of PCR technology application scope .............................................................................. 1
3. Features of LifePro Thermal Cycler................................................................................................ 1
4. Use of LifePro Thermal Cycler........................................................................................................ 1
Chapter II Features...........................................................................................................................3
1. Normal working conditions.............................................................................................................. 3
2. Transportation and storage conditions ............................................................................................ 3
3. Basic parameters ............................................................................................................................ 3
4. Software functions .......................................................................................................................... 4
Chapter III Preparations...................................................................................................................5
1. Structure diagram............................................................................................................................ 5
2. Keyboard diagram........................................................................................................................... 6
3. Instruction for buttons ..................................................................................................................... 6
4. Check before start........................................................................................................................... 7
5. Start ................................................................................................................................................ 7
Chapter IV Instruction for operation...............................................................................................8
1. How to run PCR program ............................................................................................................... 8
1.1. How to edit PCR program ........................................................................................................ 9
1.2. How to set system parameters...............................................................................................12
1.3 Gradient module temperature distribution features ................................................................. 13
2. How to input/output PCR program................................................................................................ 14
2.1 Connect to USB flash memory................................................................................................ 14
2.2 Input file from USB flash memory ........................................................................................... 14
2.3 Output file to USB flash memory.............................................................................................15
Chapter V Connection to computer..............................................................................................16
1. Software installation...................................................................................................................... 16
2. Online setting ................................................................................................................................ 16
2.1 Tool software installation ....................................................................................................... 16
2.2 Device configuration .............................................................................................................. 17
2.2.1 Connect the device to computer by network cable (cross, B type connection.) ............... 17
2.2.2 Run Network Enabler Administrator................................................................................. 17
2.2.3 Configuration ...................................................................................................................18
3. Main interface ............................................................................................................................... 22
3.1 Tool bar.................................................................................................................................. 22
3.2 Basic operation ...................................................................................................................... 22
3.3 User menu ............................................................................................................................. 25
3.4 File menu ............................................................................................................................... 26
3.5 Tool menu.............................................................................................................................. 29
Chapter VI Firmware upgrading....................................................................................................32
1. Device configuration ..................................................................................................................... 32
2. Way to upgrade instrument software ............................................................................................ 32
Chapter VII Failure analysis and handling ................................................................................... 39
1. Failure analysis and handling ....................................................................................................... 39
Attachment 1 Wiring diagram........................................................................................................ 41
Attachment 2 Value and Meaning of Parameter Setting ............................................................... 42

LifePro Thermal Cycler Introduction
－1－
Chapter I Introduction
The contents in this chapter include introduction of PCR technology, examples of PCR application,
introduction of Thermal Cycler and features of LifePro Thermal Cycler.
1. Introduction of PCR technology
Polymerase chain reaction (PCR) is a nucleic acid amplification that simulates natural DNA
replication in vitro. It takes 2 DNA chains to be amplified as template, uses a pair of artificially
synthesized oligonucleotide primer as mediator, and amplifies certain DNA fragment quickly and
specifically by thermostable DNA polymerase. Ever since PCR technology was invented by Mullis in
1985 and thermostable DNA polymerase was discovered by Erlich in 1988, this simple, fast, specific
and sensitive technology has been widely applied to life science areas with astonishing speed.
Remarkable achievements have been witnessed in cytology, virology, oncology, genetic disease,
forensic medicine, animal/plant immunology and etc. It is a milestone in contemporary molecular
biology development.
2. Example of PCR technology application scope
yResearch: gene clone, DNA sequencing, mutation analysis, gene recombination and fusion,
protein-regulating DNA sequence identification, transposition insertion site mapping, genetic
modification detection, synthetic gene construction, clone construction, carrier expression, certain
gene’s incision enzyme diversity detection and etc.
yClinical diagnosis: bacteria (spirochete, mycoplasma, Chlamydia, mycobacterium, rickettsia,
diphtheria bacillus, pathogenic E. Coli, dysentery bacillus, aeromonas hydrophilic and C. difficile
and etc.); virus (HTLV, HIV, HBV, HCV, HPVS, EV, CMV, EBV, HSV, measles virus, rotavirus,
parvovirus B19 and etc.); parasite (malaria and etc.); human genetic disease (Lesh-Nyhan
syndrome, Mediterranean anemia, hemophilia, BMD, DMD, cystic fibrosis and etc.)
yImmunology: HLA typing, T cell receptor or antibody diversity qualification, autoimmune disease
gene mapping, lymphokine quantification and etc.
yHuman genome project: DNA mark generated from scatter repetitive sequence, genetic map
construction (detect DNA, polymorphism, sperm drawing), physical map construction, sequencing,
expression map and etc.
yForensic medicine: crime scene specimen analysis, HLA-DQαanalysis and etc.
yOncology: pancreas cancer, rectal cancer, lung cancer, thyroid cancer, melanin cancer,
hematological malignant tumor and etc.
yHistology/population biology: genetic cluster research, evolution research, animal protection
research, ecology, environmental science, experimental genetics and etc.
yPaleontology: archeology and museum specimen analysis and etc.
yBotany/zoology: animal inflectional disease diagnosis, plant pathogen detection and etc.
3. Features of LifePro Thermal Cycler
LifePro Thermal Cycler is a PCR instrument with multiple functions and several applications. Below
are its features:
yUse advanced thermoelectric refrigeration technology from ITI of US and thermoelectric
refrigeration elements made under quality management of Japan, which are steady and reliable
in quality.
yTemperature can be changed as fast as 4°C /second. The speed to increase or decrease
temperature can be set as per need of experiment.
ySeveral modes for temperature control, better compatibility of reagent.
yLarge LCD screen, user-friendly operation interface, simple and easy operation
yPower-off protection available, programs can continue after power recovery.
yHeight and pressure of hot-lid can be set for different reaction tubes, which prevent reagent
evaporation and pollution effectively.
yConnect to ordinary computer by RJ45 standard interface, which is easy for software upgrading.
4. Use of LifePro Thermal Cycler
Integrating a number of advanced technologies, LifePro Thermal Cycler meets the demand of

LifePro Thermal Cycler Introduction
－2－
different PCR experiments and rank top in similar products.
LifePro Thermal Cycler is attached with:
1) 0.2 ml standard centrifugal tube
2) Strip of 8 tube
3) Strip of 12 tube
4) Full/half apron plates

LifeProThermalCycler Features
－3－
Chapter II Features
This chapter introduces the usage, transportation, storage conditions of the instrument, as well as
its basic parameters, performance and functions.
1. Normal working conditions
Ambient temperature: 10°C ～30°C
Relative humidity: ≤70%
Power supply: 220V～, 50Hz
Caution: Before using the instrument, please make sure the working condition meets the above
requirements. Especially the power line must be reliably earthed.
2. Transportation and storage conditions
Ambient temperature: -20°C ～+55°C
Relative humidity: ≤80%
3. Basic parameters
Specification TC-96/G/H(b)A
Sample size 96×0.2ml,96 micro-plate
12x8 test tube bar,8x12 test tube bar
Scope of temperature 4°C ~ 99°C
Heating Rate(max) ≥4.0°C
Cooling Rate(max) ≥4.0°C
Temperature uniformity ≤±0.2°C（55°C）
Temperature control precision ≤±0.1°C
Temperature fluctuation ≤±0.2°C
Maximum gradient 30°C
Minimum gradient 1°C
Scope of hot-lid temperature setting 30°C ~ 110°C
Scope of hot-lid working temperature 105°C ±5°C
File saving amount ≥99（pieces）
Energy consumption (maximum input power) 600W
Operation mode Continuous operation mode
Outline dimension 351mm×279mm×250mm（L×W×H）
Fuse 250V 8A Ф5×20
PC interface RJ45
Weight (kg) 10

LifeProThermalCycler Features
－4－
Caution: If the hot-lid temperature setting is lower than module temperature setting, the hot-lid
temperature shown on screen is higher than hot-lid setting.
4. Software functions
a) Edit the file (including file number, temperature and time of each segment, cycle times setting,
file view, revision and deletion, automatic file connection, gradient temperature setting, the
maximum paragraph, step and cycle times for editable file are 5, 16 and 99 respectively. The
scope for gradient temperature setting is 1°C～30°C.)
b) Save the file
c) Revise the file (adjust the rate for temperature rise/fall, refine temperature and time for cycling
automatically, and display and calibrate the time.)
d) Display the temperature, time, cycle times setting and real-time value at file running (including
sound prompt, total/rest time estimation for file running, failure protection and alarming.)
e) Stop the file running (including pause the file running, stop the file running, recover
automatically after power off.)
Caution: The above-mentioned software function is merely for reference. The software
function would be modified without notice.

LifeProThermalCycler Preparations
－5－
Chapter III Preparations
This chapter introduces the structure of instrument, keyboard for operation, function of buttons, and
preparations before start. Please get familiar with the contents in this chapter for the first time to use
this instrument.
1. Structure diagram
Note: ①Hot-lid ②Keyboard ③LCD screen
④USB interface ⑤Ventilation hole ⑥Block
⑦RJ45 interface ⑧Power switch ⑨Power socket (with fuse)
Caution: The fuse specification must be 250V, 8A, Φ5×20. Use qualified product to replace
the fuse. Contact fuse supplier or manufacturer when necessary.
①
③
④
②
⑥⑤
⑦
⑧
⑨

LifeProThermalCycler Preparations
－6－
2. Keyboard diagram
3. Instruction for buttons
Display mode switch key: switch display mode between curve
and text during running.
Plus/minus key: switch the plus/minus for program
temperature editing and time refining.
Cursor left/right key: press once to move the cursor left/right.
Cursor up/down key: press once to move the cursor up/down.
Confirmation key: press button to accept the setting value on
screen at file setting.
Menu key: press button to accept the item above the button on
screen.
Character key: to input
characters, press button
continuously to input
letters.

LifeProThermalCycler Preparations
－7－
4. Check before start
Check the following before insert the plug and power on the instrument:
1) Whether the voltage of power supply matches the voltage required by instrument. (see Chapter
II for requirements of power supply)
2) Make sure the power line pug has inserted in power socket reliably.
3) Earth the power line reliably.
5. Start
Power on, (turn power to “-”), the amplifier gives a “du-du” sound, and the fan starts to run, which
indicates the power supply is available.
“Self testing••••••” is shown on screen, and then the instrument takes about 1 to 2 minutes for self
testing. Please wait with patience. The main interface appears if no problem is found, then you can
edit, view, modify or delete the PCR amplification file.

LifePro Thermal Cycler Instruction for operation
－8－
Chapter IV Instruction for operation
This chapter explains how to edit, view, modify and delete the PCR amplification file, how to run
PCR amplification file, and how to set the instrument system parameters in detail.
Warning! If the instrument sound or display is abnormal after start, or failure alarm and
indication is shown during self testing, please turn off the power immediately and
contact the supplier.
Caution: If the sample amount for test is less than countersink amount on module, please
distribute the sample test tube as uniform as possible. In this way it ensures that the
hot-lid presses at the test tube top steadily at file running, the module bears uniform
load, and the temperature change in test tubes keeps uniform.
Caution: Turn the knob at anti-clockwise direction as per diagram before covering the lid
(clockwise rotation for pressing, anti-clockwise rotation for loosing) until a
“dang-dang” sound is heard.
Cover the lid and turn the knob at clockwise direction until a “dang-dang” sound is
heard, and then turn another step.
When a “dang-dang” sound is heard when knob is rotated at first, regardless of
clockwise or anti-clockwise, press the unlocking device by finger, turn the knob at
the opposite direction for two steps, and then rotation knob on unlocking device can
be released.
1. How to run PCR program
Power on, the thermal cycler gives a “du-du” sound, which indicates the power supply is available.
“Self testing ……” is shown on screen, and the instrument takes about 1 to 2 minutes for self testing.
Please wait with patience. The main interface appears if no problem is found.
Shown on the upper left of main interface is
the status of hot-lid setting.
Shown in the middle of main interface is the
information such as the Bioer information,
software version, module type, default
program, default user, control mode, sample
amount and so on.
Press “File” to access the interface for
file list.
Press “System” to access the interface
for system parameter setting.
Press “Run” to confirm running mode
and test tube type if file password is not
asked, press “Run” to bounce the
interface below if file password is asked.
Press number key to input the password.
Press “Back” to return to main interface.
Press “Accept” to access the interface for file running if Control Mode is Block now, press
“Accept” to bounce the dialog box for test tube volume setting if Control Mode is Tube now.
Hotlid=off 2009-05-1 10:05:00
BIOER
Bioer Technology Co.,Ltd
Version: 2008-1.1
Block: B-96G
DefaultUser: BIOER
DefaultFile: PCR1
Control Mode: Tube
File UsbLink S
y
stem Run
Main interface

LifePro Thermal Cycler Instruction for operation
－9－
Hotlid=off 2009-05-1 10:05:00
BIOER
Bioer Technology Co.,Ltd.
Version: 2008-1.1
Block: **×**
DefaultUser:BIOER
DefaultFile:PCR1
Control Mode: Tube
Back Run
Hotlid=off 2009-05-1 10:05:00
BIOER
Bioer Technology Co.,Ltd
Version: 2008-1.1
Block: **×**
DefaultUser: BIOER
DefaultFile: PCR1
Control Mode: Tube
Back Next
Press to +/- select the test tube type in this
interface.
Press “Back” to return to main interface.
Press “Next” to access the interface for file
running if Control Mode is Block now,
press “Next” to bounce the interface below
if Control Mode is in Tube mode now.
Press Character key to input the micro liter
amount of sample size. Different sample sizes
have different overshoot temperature and
lasting time when temperature control mode is
Tube.
Press “Back” to return to main interface.
Press “Run” to access the interface for file
running.
1.1. How to edit PCR program
Press “File” in main interface to access the
interface for file list. As shown in figure, there
are 3 files in the file list. Use the up/down key
to select user when blinking bar blinks in the
User column, and the file for selected user is
shown in the File Name column; shift to File
Name by right click, then the blinking bar
blinks in the File Name column, select
corresponding User file by up/down key.
Press “Edit” to edit the file pointed by
cursor.
Press “New File” to edit a new file.
Press “Delete”, it asks “Confirm delete
file?” select Delete or Back as per
information.
Press “Run” to confirm running mode and
test tube type if file password is not asked, press “Run” to bounce the dialog box below if file
password is asked:
Input Password:
-
Hotlid=off 2009-05-1 10:05:00
No. User File Name Save Time
1 BIOER1* PCR1 2009-05-1
2 BIOER2 PCR2* 2009-05-1
3 BIOER3 PCR3 2009-05-1
Edit NewFile Delete Back Run
Test tube type selection
Select Tube Type:
0.2ml
Select Sample Volume:
050
μl
Interface for file list

LifePro Thermal Cycler Instruction for operation
－10－
Press “Run” to access the interface for file running when Control Mode is Block; press “Run” to
bounce the dialog box below if Control Mode is Tube now:
It is the same with main interface, confirm the password, input the sample type and micro liter
amount of sample size.
Note:
1. “*” on upper right of user and file indicates this file is the default for system running.
2. File can be modified in the interface for system parameter setting.
Interface for file editing
Segment and paragraph can be edited in this
interface. The file is comprised of segments
and paragraphs, while segments are
contained in paragraphs. Cycle times can be
set for segments in paragraphs (see figure
below for relation). Press up/down or
left/right key to move the cursor, the place
where cursor is are highlighted. Press
character key to change the parameter
setting. Press plus/minus key to change the
plus/minus symbol.
Press “+Seg.” key for segment editing,
where you can set temperature (Temp),
lasting time (Time), rate for temperature
rise/fall (Ramp), temperature increment
in each cycle (+Temp) and time
increment in each cycle (+Time). Press
“Delete” to delete the segment where cursor is.
Press “+Cycle” key for paragraph editing, where you can set cycle amount and cycle starting
segment. The figure shows that it cycles from segment 1 to segment 3, and cycles for 10 times.
Repeat to press “+Cycle” key to add paragraphs (maximum is 5). Press “Delete” to delete the
current paragraph immediately. Move the cursor up/down to enter previous or next paragraph.
Press “Save” key to access the interface for file saving.
Press “Back” key to return to the interface for file list.
Special caution: 1. Please save the new edited PCR program before running, or the file can’t run.
2. If “Time” is set as “--:--”, hot-lid is closed automatically when module
temperature control works.
Hotlid=off 2009-05-1 10:05:00
100
75
50
25
0
Temp 1c 95.0 2c 55.0 3c 72.0 4 04.0
Time 01:00 02:00 01:45 00:00
Seg. Temp(C) Time Ramp(C/s) +Temp +Time
1c 95.0 01:00 #.# +0.0 +0:00
2c 55.0 02:00 #.# +0.0 +0:00
3c 72.0 01:45 #.# +0.0 +0:00
4 04.0 00:00 #.# +0.0 +0:00
Cycle1：×10 From 01 to 03
+Seg. +Cycle Delete Back Save
Relation of segment and paragraph in the file
Select Sample Volume:
050
μ
l
Cycle1 ×** From ** to **
Se
g
. 1
Se
g
. 2
Se
g
. 16
Cycle2 ×** From ** to **
·
·
·
·
·
·
Cycle5 ×** From ** to **
File

LifePro Thermal Cycler Instruction for operation
－11－
Press character key in this interface to input
file name, press confirmation key to move
the cursor forward. Select User and File
Name by up/down key. Delete character by
left key, move cursor by right key. Press
Caps Key to shift between capital and lower
case. At upper left on screen it indicates
capital or lower case (as shown in the figure
abc).
Press “Main” key to return to main
interface
Press “Save” key to save the file. When
file is saved normally, the system shows
“File have been saved. Please continue
another operation”. Press confirmation
key to make it disappear. If file has the
same name with another, the system
asks to name again (Rename) or continue to save (Save).
Press number key to set and save password.
Press “Run” to access the interface for file running if Control Mode is Block now, press “Run” to
bounce the dialog box below if Control Mode is Tube now:
It is the same with main interface; confirm the password; input the sample type and micro liter
amount of sample size.
Caution: 1. File name and user name are not case sensitive.
2. Please remember the password.
3. The password should be 0~9 numeric characters.
As the file runs normally, “•••” at Now
Running keeps blinking. The system
shows “File run over” when running is
completed.
Press “Stop”, it shows “Confirm stop
running?”
Select stop (stop) or continue (Start)
as per information.
Press “Pause”, it shows “Now pause
running”, you can select continue
(Start) as per information.
Press “View File” to view the edited
PCR program”.
Press “Skip” to skip the current
temperature section and go to the
next temperature.
Hotlid=off 2009-05-1 10:05:00
abc
User：PCR1
File Name：PCR1
Password：PC****
Password Check：PC****
Main Caps Save Run
Interface for file saving
Select Sample Volume:
050
μl
File running interface 1
Hot lid=off 2009-05-1 10:05:00
User/File: Bioer/Bioer
Temp: 95.0C
Time: 01.00
Remain Time: 00h56m
Stop Pause Start Skip Run

LifePro Thermal Cycler Instruction for operation
－12－
Press to shift between running
interface 1 and running interface 2.
1.2. How to set system parameters
Press “System” in main interface to access
the interface for system parameter setting.
Press confirmation key to move the cursor
to the next item. Roll and select the items
with “ ” by cursor up/down key, or press
character key to input information for other
items, press cursor left/right key to move
the cursor.
Press “Back” to return to main menu.
Press “Save” to save parameters.
Special caution: press “Save” to confirm
after system parameter setting or the system
parameter won’t change.
1.2.1 “Date”, “Time” indicate the default system time after system start.
1.2.2 “Default File”, “Default User” indicates the default running program name and user after
system start.
1.2.3 “Control Mode” indicates the default temperature control mode after system start, including
“Block” and “Tube” choices.
a) “Block” mode only concerns the temperature control for module during temperature
rise/fall. Now that the temperature rise/fall of reagent lags behind the module, the actual
temperature change of reagent is different from the program we set. The actual time that
the reagent reaches the set temperature is far less than the set time (see figure below for
relation between module temperature and reagent temperature).
Hotlid=off 2009-05-1 10:05:00
Date: 03-10-22
Time: 14:53:19
Default User: PCR1
Default File: BIOER
Control Mode: Block
Hotlid: OFF
Key Sound: Yes
Run End Sound: Yes
Back Save
Interface for system parameter setting
Hotlid=off 2009-05-1 10:05:00
100
75
50
25
0
Temp 1c 95.0 2c 55.0 3c 72.0 4 04.0
Time 1:00 02:00 01:45 00:00
File Name:PCR1
User:BIOER NowRunning•••
1c Temp=00.0 Time=00:00 Cycle1 01 of 10
Total Time: 00h 56m Remainig Time:00h 56m
Stop Pause Start Skip View File .
module
reagent
File running interface 2

LifePro Thermal Cycler Instruction for operation
－13－
b) “Tube” mode considers the temperature rise/fall of reagent that lags behind the module.
There is an overshoot when it reaches the set temperature so that the reagent reaches
the set temperature faster. At the set time same with “Block” mode, obviously the actual
time that reagent reaches set temperature is prolonged. (See figure below for relation
between module temperature and reagent temperature.
1.2.4 “Hotlid” indicates the on/off status of hot-lid.
1.2.5 “Key Sound” and “Run End Sound” indicate the sound to press key and the sound at program
finish respectively.
See attachment 2 (Value and Meaning of Parameter Setting) of this manual for specific index
of each parameter above
Caution: 1. Press “Save” to confirm the system parameter setting, or the system parameter
won’t change.
2. It calibrates the clock inside the instrument as per Beijing time upon ex-factory.
3. It is recommended to check the date and time again for the first time to use.
1.3 Gradient module temperature distribution features
The gradient module uses 3-line temperature control unit. The gradient temperature is distributed as
curve, as shown in figure below (the set temperature is 40°C and the set gradient temperature is
20°C.) The temperature gradient in each hole row on module is nor uniform. The temperature
difference on hole rows at center is higher than the that at outside. Therefore, at gradient module
operation, the temperature of each hole row shown on instrument governs.
Gradient graphs
40
42
44
46
48
50
52
54
56
58
60
123456789101112
Column(left to right)
Temperature[ ℃]
module
reagent

LifePro Thermal Cycler Instruction for operation
－14－
2. How to input/output PCR program
2.1 Connect to USB flash memory
Press “UsbLink” in main interface to access
the interface for USB connection. If USB
flash memory is inserted, as the figure at
left shows:
Press “Back” to return to main
interface.
Press “Input” to select file input from
USB flash memory, it shows USB user
list.
Press “Output” to select to output file
USB flash memory, it shows local file
user list.
If no USB flash memory is inserted, as the
figure at left shows, press “Back” to return
to main interface.
2.2 Input file from USB flash memory
Press “Back” to return to interface of
USB flash memory connection.
Press cursor up/down to select copy
file user.
Turn page by cursor left/right if user
number is more than 30.
Press “View” to see USB file list.
Hotlid=off 2009-05-1 10:05:00
User1
User2
Press Left / Right Key to Previous / Next Page.
Back View
Hotlid=off 2009-05-1 10:05:00
The Instrument has Connected UsbDisk.
Please Choose Input or Output File.
Press F1 to Back
Press F4 to Input File
Press F5 to Output File…
Back Input Output
USB connection interface
Hotlid=off 2009-05-1 10:05:00
No disk connection.
Press F1 to Back
Back k
Interface of USB connection fail

LifePro Thermal Cycler Instruction for operation
－15－
Press “Back” to return to interface of
USB flash memory user list.
Press cursor up/down to select copy
file.
Turn page by cursor left/right if user
number is more than 30.
Press “Copy” to input file.
If copy is correct, it shows the dialog
box below:
If not, it shows:
2.3 Output file to USB flash memory
Press “Back” to return to interface of
USB flash memory connection.
Press cursor up/down to select copy
file user.
Turn page by cursor left/right key if
user number is more than 30.
Press “View” to see device user file list.
Press “Back” to return to interface of
device user list.
Press cursor up/down key to select
copy file.
Turn page by cursor left/right key if
user number is more than 30.
Press “Copy” to output file.
If copy is correct, it shows the dialog
box below:
If not, it shows:
Caution: 1. USB reads/writes 500 users, each user has 2,000 files.
2. USB reads/writes bioer file at USB root directory in fixed directory.
3. Each user is the next subfolder under bioer file.
4. Don’t plug USB flash memory in/out quickly. To protect device and USB flash
memory from damage, the device will terminate the USB function until next run if
USB flash memory is plugged in/out quickly.
5. The operation for dual module is same. It only connects USB flash memory at
start interface of two modules.
Hotlid=off 2009-05-1 10:05:00
File1
File2
Press Left / Right Key to Previous / Next Page.
Back Copy k
Hotlid=off 2009-05-1 10:05:00
Local
User1
User2
Press Left / Right Key to Previous / Next Page.
Back View
Hotlid=off 2009-05-1 10:05:00
Local
File1
File2
Press Left / Right Key to Previous / Next Page.
Back Copy k
USB file list
FileSaved
File in
p
uterror.
FileSaved
File output error.
Device file list

User’s Manual of LifePro Thermal Cycler Connection to computer
－16－
Chapter V Connection to computer
1.Software installation
Click “Setup.exe” in accompanied disc; install the LifePro computer connection program, which is
able to control 30 LifePro instruments on one computer.
2.Online setting
LifePro is equipped with embedded online module inside and tools for setting are available.
2.1 Tool software installation
Step 1, click and run nead_setup.exe. Step 2, click Next.
Step 3, select destination location, click Next. Step 4, Click Next.
Step5,clickInstall. Step 6, complete installation, click Finish.