Beckman Coulter Optima LE-80K User manual

Published by the Centrifuge Instrument Systems Development Center of Beckman Coulter, Inc., Palo Alto, California 94304
LLE7/8-IM-5
Instruction Manual
Optima™LE-80K
Preparative Ultracentrifuge
TM

Symbol
Symbol
Symbole
Símbolo
Simbolo
Title / Titel / Titre / Titulo / Titolo /
Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione
Attention, consult accompanying documents
Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate
On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)
Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)
Protective earth (ground)
Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra
Earth (ground)
Erde
Terre
Tierra
Scarica a terra
!

SAFETY NOTICE
This safety notice summarizes information basic to the safe operation of the equipment
described in this manual. The international symbol displayed above is a reminder that all
safety instructions should be read and understood before installation, operation, mainte-
nance, or repair of this instrument. When you see the symbol on other pages, pay special
attention to the safety information presented. Observance of safety precautions will also
help to avoid actions that could damage or adversely affect the performance of the
equipment.
Other symbols may also be displayed on the equipment. These are reproduced and
described on the inside of the front cover.
Safety During Installation and/or Maintenance
This instrument is designed to be installed by a Beckman Coulter Field Service
representative. Installation by anyone other than authorized Beckman Coulter personnel
invalidates any warranty covering the instrument.Also, if the instrument needs to be
moved, a Beckman Coulter Field Service representative must reinstall and relevel the
instrument in its new location.
Any servicing of this equipment that requires removal of any covers can expose parts
which involve the risk of electric shock or personal injury. Make sure that the power
switch is turned off and the instrument is disconnected from the main power source,
and refer such servicing to qualified personnel.
Electrical Safety
To reduce the risk of electrical shock, this instrument uses a three-wire electrical cord and
plug to connect this equipment to earth-ground. Make sure that the matching wall outlet
receptacle is properly wired and earth-grounded.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid
may get into the instrument and damage electrical or mechanical components.
Safety Against Risk of Fire
Fuses protect certain electrical circuits within this instrument against overcurrent condi-
tions. For continued protection against the risk of fire, replace only with the same type and
rating specified.
This instrument is not designed for use with materials capable of developing flammable
or explosive vapors. Do not centrifuge such materials (for example, chloroform or ethyl
alcohol) in this instrument nor handle or store them near the centrifuge.
!

Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the Beckman Coulter rotors and accessories designed for use in this
instrument.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop a rotor by hand.
• Do not move the centrifuge while the drive motor is spinning.
• In the event of a power failure, do not attempt to retrieve the sample from the instru-
ment for at least one hour. Then follow the instructions for recovery of the sample
under
TROUBLESHOOTING AND MAINTENANCE
.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic,
toxic, or radioactive. Such materials should not be used in this instrument, however, unless
all necessary safety precautions are taken .
• Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that they are free of micro-organisms. some of the most virulent—
Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacterium, and certain
systemic fungi—further emphasize the need for aerosol protection. Handle other infec-
tious samples according to good laboratory procedures and methods to prevent spread
of disease. Because spills may generate aerosols, observe proper safety precautions for
aerosol containment. Do not run toxic, pathogenic, or radioactive materials in a rotor
without taking appropriate safety precautions. Biosafe containment should be used
when Risk Group II materials (as identified in the World Health Organization Labora-
tory Biosafety Manual ) are handled; materials of a higher group require more than one
level of protection.
•Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the instrument and accessories before
requesting service by Beckman Coulter Field Service.

Published by the Centrifuge Instrument Systems Development Center of Beckman Coulter, Inc., Palo Alto, California 94304
LLE7/8-IM-5
January 2003
Instruction Manual
Optima™LE-80K
Preparative Ultracentrifuge
© 2003 Beckman Coulter, Inc.
Printed in the U.S.A.

I.S. EN ISO 9001

Contents
iii
Page
INTRODUCTION
Instrument Certification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Scope of this Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
Radio Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
Installation Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii
SECTION 1: DESCRIPTION
Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Standby Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Hardkeys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Run Status Indicators. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Diagnostic Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Vacuum System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Temperature Sensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Temperature Control System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Overspeed System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Rotor Energy System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
Drive. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
Name Rating Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
SECTION 2: RUN PREPARATION
Entering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Standard Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Keypad. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

_')a
(Clear Entry) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
ENTER/RECALL

iv
Page
Parameter Keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

_74))(a
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

_8-1)a
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
(Accumulated Centrifugal Effect) . . . . . . . . . . . . . . . . . . . . 2-4

_,30(a
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

_8)14a
(Temperature). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

_%'')0a
(Acceleration). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

_()')0a
(Deceleration). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Programmed Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7

_463+a
(Program) and

_7%:)a
. . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Activation Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8

_:%'991a
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8

_78%68a
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9

_7834a
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9
Delayed Start Program Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9
Entering Program 0 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10
Running Program 0 with a Second Program . . . . . . . . . . . . . . . . 2-10
SECTION 3: RUN PROCEDURE
Standard Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Programmed Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Delayed Start Program Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Points to Remember . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
SECTION 4: TROUBLESHOOTING AND MAINTENANCE
In Case of Power Failure During the Run . . . . . . . . . . . . . . . . . . . . . . 4-1
Retrieving Your Sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Diagnostic Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
Sterilization and Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
Storage and Transportation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
Supply List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
Warranty
ω
2t

Illustrations
v
Tables
Page
Figure 1-1. The Control Panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Figure 1-2. The Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Figure 4-1. Removing the Control Head Cover. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Figure 4-2. Depressing the Latch to Loosen the Front Panel . . . . . . . . . . . . . . . . . . 4-4
Figure 4-3. Interior View of Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Figure 4-4. Reinstalling the Front Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Figure 4-5. Location of Diagnostic Messages on the Control Panel. . . . . . . . . . . . . 4-7
Page
Table 1-1. Run Status Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Table 2-1. Vacuum LEDs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
Table 4-1. Diagnostic Troubleshooting Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8

Introduction
vii
INSTRUMENT CERTIFICATION
To assure full system quality, the Beckman Coulter Optima™
LE-80K ultracentrifuges have been manufactured in an NSAI-
registered ISO 9001 facility. They have been designed and tested to
meet the laboratory equipment safety standards and regulations (only
when used with Beckman Coulter rotors) of CSA and CE Mark.
SCOPE OF THIS MANUAL
This manual is designed to familiarize you with the Beckman Coulter
Optima LE-80K Preparative Ultracentrifuge—its functions, specifi-
cations, operation, and routine operator care and maintenance.
•The following introductory pages contain the instrument specifica-
tions and preinstallation requirements.
• Section 1 provides a brief description of the instrument, the
operating controls, and indicators.
• Section 2 explains how to use the control panel to enter run
parameters for standard and programmed operation.
• Section 3 covers operating procedures for both kinds of runs.
• Section 4 lists possible malfunctions, together with probable
causes and corrective actions, and maintenance procedures.
We recommend that you read this entire manual, especially the
SAFETY NOTICE
and all safety-related information, before operating
or performing maintenance on this instrument.

viii
Introduction
➠NOTE
If the Optima LE-80K is used in a manner other
than specified in this manual, the safety and
performance of this instrument could be
impaired. Further, the use of any equipment
other than that intended for use by Beckman
Coulter has not been evaluated for safety. Use of
any equipment not specifically recommended in
this manual is the sole responsibility of the user.
CONVENTIONS
NOTES, CAUTIONS, AND WARNINGS
Certain symbols are used throughout this manual to call attention to
safety-related and other important information. The symbols used and
their definitions are as follows:
➠NOTE
Used to call attention to information that should
be followed during installation, use, and/or
servicing of the equipment.
!CAUTION
Used to indicate a potentially hazardous situa-
tion which, if not avoided, may result in minor or
moderate injury and/or mechanical damage. It is
also used to alert against unsafe practices.
WARNING
!
Used whenever an action or condition may
potentially cause serious personal injury or
loss of life. Mechanical damage may also
result.

ix
Introduction
WARNING
Indicates high voltage or risk of electric
shock. Refer servicing of all areas displaying
this symbol to service personnel.
TYPOGRAPHIC CONVENTIONS
Certain typographic conventions are used throughout this manual to
distinguish names of user interface components, such as keys and
displays.
• Key names (for example,

_78%68a
or ) appear in
capital letters within boxes.
• Display names (for example, TEMP°C or SPEED-RPM ) appear
in bold type.
RADIO INTERFERENCE
This instrument has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environ-
ment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with
this instruction manual, may cause interference to radio communica-
tions. Operation of this equipment in a residential area may cause
interference, in which case the user will be required to correct the
interference at his or her own expense.
ENTER/RECALL

x
Introduction
CANADIAN REGULATIONS
This digital apparatus does not exceed the ClassA limits for radio
noise emissions from digital apparatus as set out in the radio interfer-
ence regulations of the Canadian Department of Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques
dépassant les limites applicables aux appareils numériques de Classe
A prescrites dans le reglement sur le brouillage radioelectrique édicté
par le Ministère des Communications du Canada.

xi
Introduction
SPECIFICATIONS
Only values with tolerances or limits are guaranteed data. Values
without tolerances are informative data, without performance
guarantee.
CONTROL FEATURES
Speed
Set speed . . . . . . . . . . . . . . . . . . . . . 1000 rpm to 80 000 rpm in increments of 100 rpm
Speed control . . . . . . . . . . . . . . . . . . actual rotor speed will be ±50 rpm of the set speed (above 1000 rpm)
Speed display . . . . . . . . . . . . . . . . . . indicates rotor speed in increments of 10 rpm at speeds below 1000 rpm,
and increments of 100 rpm at speed above 1000 rpm
Time
Set time . . . . . . . . . . . . . . . . . . . . . . up to 99 hours and 59 minutes; HOLD for runs of unspecified length
Time display. . . . . . . . . . . . . . . . . . . indicates time remaining in timed runs, time elapsed in HOLD runs, or
estimated time remaining in ω
2
t runs
Rotor Temperature
Set temperature . . . . . . . . . . . . . . . . 0 to 40°C in increments of 1°C
Temperature control. . . . . . . . . . . . . ±1°C of set temperature
Temperature display. . . . . . . . . . . . . indicates rotor temperature in increments of 0.1°C
Temperature stability . . . . . . . . . . . . within 0.3°C of set after equilibrium is reached
STOP
VACUUM–
µ
SPEED–RPMTIME–HR:MIN TEMP–˚C
POWER OFF
X 10,000
SAVE
PROGHOLD
w
2
t
DECEL
ACCEL
VACUUM
START
STOP
TIME
TEMP
CE
0
123
456
789
SPEED
ENTER/RECALL

xii
Introduction
ω
2
t Integrator
Set ω
2
t . . . . . . . . . . . . . . . . . . . . . . . up to 9.99 × 10
14
radians squared per second
ω
2
t display . . . . . . . . . . . . . . . . . . . . shows accumulated centrifugal force to three significant digits
(in exponential notation)
Acceleration . . . . . . . . . . . . . . . . . . . . selection from two profiles: maximum acceleration from 0 rpm to set speed,
or slow acceleration from 0 to 500 rpm, followed by maximum acceleration
to set speed
Deceleration . . . . . . . . . . . . . . . . . . . . selection from three profiles: full dynamic braking to 0 rpm, slow (full brake
to 500 rpm followed by reduced braking to a gentle stop), or no brake
Operation . . . . . . . . . . . . . . . . . . . . . . standard, programmed, or delayed start; instrument memory can store up to
nine individual programs
OPERATIONAL FEATURES
Revolution Counter . . . . . . . . . . . . . . displays accumulated total number of rotor revolutions in tens of thousands
Barrier Ring . . . . . . . . . . . . . . . . . . . . 41-mm (1.63-in.) heat-treated steel alloy armor ring surrounded by a 12-mm
(0.50-in.) steel vacuum chamber to provide full protection for the operator
Door . . . . . . . . . . . . . . . . . . . . . . . . . . . 17.5-mm (0.69-in.) high-strength structural steel
Vacuum . . . . . . . . . . . . . . . . . . . . . . . . diffusion pump in series with a mechanical pump reduces chamber pressure
to below 5 microns (0.7 Pa)
Instrument Classification . . . . . . . . . R (uses all Beckman Coulter preparative rotors except Types 35 and
42.1 rotors with serial numbers 1299 or lower); not equipped for zonal or
continuous-flow operation
INSTALLATION REQUIREMENTS
!CAUTION
Do not attempt to install or turn on the power
to the instrument. Its purchase price includes
installation by Beckman Coulter Field Service.
Installation by anyone other than authorized
Beckman Coulter personnel invalidates the
warranty.

xiii
Introduction
PREINSTALLATION REQUIREMENTS
Preinstallation requirements have been sent prior to shipment of the
instrument. (Copies are also attached to the shipping container.) The
following information is provided in case the ultracentrifuge must be
relocated. Contact your Beckman Coulter Field Service representa-
tive to adjust and level the instrument if it must be moved. (The pads
on each leveling leg are designed to prevent possible rotation of the
instrument in case of a rotor mishap.)
Electrical Data
Instrument rating: 220 to 240 VAC, 50 Hz, 20 A
200 to 240 VAC, 50/60 Hz, 20 A
Power line range: 180 to 264 VAC, 60 or 50 Hz (single-phase), 30 A
To reduce the risk of electrical shock, this equipment uses a three-
wire electrical cord (1.8 m; 6 ft) and plug to connect the equipment
to earthground. In regions where the instrument is supplied with
an unterminated cord, a plug that meets local electrical and safety
requirements must be supplied. (Contact your local Beckman Coulter
office for specific information regarding these requirements.) See the
Table below for the required wire connections. Make sure that the
matching wall outlet receptacle is properly wired and earth-grounded.
Measured
Line Voltage
Wall Outlet:
Hubbell 9330,
Bryant 96-30-FR,
or Equivalent
(NEMA 6-30 R)
30-ampere
Circuit
Protector
Earth
Ground North
American
Plug
30-ampere
Circuit
Protector

xiv
Introduction
Required Wire Connections
To ensure safety, the instrument should be wired to a remote emer-
gency switch (preferably outside the room where the centrifuge is
housed, or adjacent to the exit from that room), in order to disconnect
the instrument from the main power source in case of a malfunction.
Space
Locate the Optima LE-80K in a clean, safe, uncluttered environment.
Be sure to provide a 5.1-cm (2-in.) clearance on each side of the
instrument, as the feet extend about 5.1 cm (2 in.) beyond the instru-
ment. (The pads under each foot have been designed to prevent
possible rotation of the instrument in the event of a rotor mishap.)
A 15.2-cm (6-in.) clearance is required at the rear of the instrument
for servicing the control panel and to ensure sufficient air ventilation
during operation.
The instrument will operate within specifications in a laboratory with
ambient temperatures ranging from 15 to 40°C.
WARNING
!
Do not place the instrument near areas
containing flammable reagents or
combustible fluids.
Wire Insulation
Color Terminal
Symbol
Harmonized North American
Green/Yellow Earth ground
Light Blue Neutral N L
Brown Live or Line L L

xv
Introduction
PHYSICAL DATA
Weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 465 kg (1025 lb)
Height (overall) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120.7 cm (47.5 in.)
Width. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94.0 cm (37 in.)
Depth. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67.3 cm (26.5 in.)
Clearances . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.1 cm (2 in.) sides;
15.2 cm (6 in.) rear
Finish. . . . . . . . . . . . . urethane paint on top surface; general-purpose paint
on other surfaces; coated polycarbonate finish on control panel
Maximum heat dissipation into the room . . . . . . . . . 1.0 kW (3400 Btu/hr)
Humidity restrictions. . . . . . . . . . . . . . . . . . . . . . . <95% (non-condensing)
Noise level measured 0.91 m (3 ft) in front of the instrument . . . . . .57 dBa
Installation category . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . II
Pollution degree. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
cm
in.
94.0
37
5.1
2
5.1
2
67.3
26.5
15.2
6

1
Description
1-1
This section describes major instrument components.
POWER
A circuit breaker, labeled I (on) and O (off), is located on the right
side of the instrument and controls electrical power to the instrument.
Power may be left ON at all times (see STANDBY MODE below)
except in the case of an emergency or when maintenance is required.
A red POWER OFF button is provided on the neck of the control
head for your convenience (see Figure 1-1). Pressing the POWER
OFF button trips the circuit breaker to the off position. To return
power to the instrument, the circuit breaker must be returned to the
up position.
STANDBY MODE
When the instrument is at rest, it automatically goes into standby
mode. In this condition, only minimal power is used by the instru-
ment. To restore the instrument to fully operational mode, press
any key.
CONTROL PANEL
Figure 1-1 shows the Optima LE-80K control panel, which includes a
video display and keys for entering run information.

1-2
Description
Figure 1-1. The Control Panel
HARDKEYS
•Parameter hardkeys are used to enter the run conditions. They are
_74))(a, _8-1)a, and _8)14a to the left of the keypad and _%'')0a,
_()')0a, , _,30(a, _463+a, and _7%:)a along the bottom of the
display area.
• Activation keys control specific ultracentrifuge functions. These
are _:%'991a,_78%68a and _7834a, located to the right of the
keypad.
The keypad is used to enter numerical values. It consists of
the numbers 0 through 9, a _')a (clear entry) key, and an
key.
DISPLAYS
Digital displays indicate rotor speed, run time, rotor temperature, the
ω
2t value (when in that mode), and the selected program number (if
used). The displays serve a dual purpose.
VACUUM–µ
SPEED–RPM TIME–HR:MIN TEMP–˚C
POWER OFF
X 10,000
Location of
Diagnostic
Messages
Parameter
Keys
Parameter
Keys Keypad Activation
Keys
SAVEPROGHOLDw2tDECELACCEL
VACUUM
START
STOP
TIME
TEMP
CE
0
123
456
789
SPEED
ENTER/RECALL
ω
2t
ENTER/RECALL