Thermo Scientific HESI-II Probe User manual

HESI-II Probe
User Guide
70005-97000 Revision C April 2009

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Release history: Revision A June 2008, Revision B September 2008, Revision C April 2009
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Thermo Scientific HESI-II Probe User Guide v
C
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Precautions for Handling the HESI-II Probe . . . . . . . . . . . . . . . . . . . . . . . . . .viii
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .ix
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Chapter 2 Functional Description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
Chapter 3 Removing and Installing the HESI-II Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Removing the HESI-II Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Installing the HESI-II Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Adjusting the Probe Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Chapter 4 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
Flushing the Sample Transfer Line, Sample Tube, and HESI-II Probe . . . . . . . 19
Replacing the Needle Insert. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Chapter 5 Replaceable Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
Contents

Thermo Scientific HESI-II Probe User Guide vii
P
Preface
This HESI-II Probe User Guide provides you with information on using the
heated-electrospray ionization probe. It also provides procedures for installing and
maintaining the HESI-II probe.
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.
Safety and special notices include the following:
CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION
notice is accompanied by an appropriate CAUTION symbol.
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Note Highlights information of general interest.
Tip Highlights helpful information that can make a task easier.

Preface
viii HESI-II Probe User Guide Thermo Scientific
Precautions for Handling the HESI-II Probe
When operating the mass spectrometer in the heated-electrospray mode, do not touch the
heated surfaces of the HESI-II probe or the Ion Max API source housing. Touching the
heated surfaces of the probe or the source housing can cause burns when the probe is
operating at typical temperatures of 350 to 450 °C.
When operating the mass spectrometer in the heated-electrospray mode, if you observe liquid
leaking from the sample inlet, place the mass spectrometer in Standby mode before you
tighten the fitting to eliminate the leak.
CAUTION AVOID BURNS. At typical operating temperatures (350 to 450 °C), the
HESI-II probe can severely burn you. Before removing the probe from the API source
housing, allow the probe to cool to room temperature (for approximately 20 minutes)
before you touch it.
CAUTION AVOID ELECTRIC SHOCK. Do not tighten the probe sample inlet fitting
to eliminate a liquid leak while the mass spectrometer is in operation. If you touch liquid
leaking from the probe sample inlet while the mass spectrometer is in operation, you
might receive an electric shock.
A
S
Hot surface caution
General caution
Hot surface caution
Avoid touching liquid leaking from
the sample inlet while the mass
spectrometer is in operation.

Preface
Thermo Scientific HESI-II Probe User Guide ix
Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need.
YTo contact Technical Support
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YTo contact Customer Service for ordering information
YTo copy manuals from the Internet
Go to mssupport.thermo.com and click Customer Manuals in the left margin of the
window.
YTo suggest changes to documentation or to Help
• Complete a brief survey about this document by clicking the link below. Thank you
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