Hettich ZENTRIMIX 380 R User manual

Read the instructions prior to performing any task!
Translation of the original operating manual
ZENTRIMIX
Operating manual
1 / 88
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Rev. 03 / 02.2021
AB3200

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Andreas Hettich GmbH & Co. KG
Föhrenstraße 12
78532 Tuttlingen
Germany
+49 (0)7461 / 705-0
+49 (0)7461 / 705-1125
[email protected]
[email protected]
www.hettichlab.com
© 2017

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Table of contents
Table of contents
1 Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Symbol on the shipping carton label. . . . . . . . . . . . . . . . . . . 8
1.3 Personal protective equipment. . . . . . . . . . . . . . . . . . . . . . . 8
1.4 Personnel qualication. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.5 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.6 Replacement parts/consumable material. . . . . . . . . . . . . . . 10
1.7 Scope of delivery. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
1.8 Return shipments. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2 Safety information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3 Device description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4 Transport and storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5 Commissioning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.1 Unpacking the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.2 Setting up, connecting and switching on the device. . . . . . . 23
6 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.1 Operating elements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.2 Opening the lid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.3 Closing the lid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.4 Quick-stop function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.5 Loading the rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6.5.1 Loading a rotor for classical centrifugation. . . . . . . . . . . . . 32
6.5.2 Loading a rotor for dual centrifugation. . . . . . . . . . . . . . . . 33
6.5.3 Handling of bio-safety systems. . . . . . . . . . . . . . . . . . . . . 34
6.6 Starting centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
6.7 Dual centrifugation: Validating application. . . . . . . . . . . . . . . 36
6.8 Emergency unlocking. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
6.9 Removing and installing the rotor. . . . . . . . . . . . . . . . . . . . . 39
6.10 Cooling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7 Software description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7.1 Program settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7.1.1 Calling up/loading program (RCL 1-99). . . . . . . . . . . . . . . 44
7.1.2 Save programs (STO 1-99). . . . . . . . . . . . . . . . . . . . . . . . 44
7.1.3 Calling up/loading a program link (RCL A-Z). . . . . . . . . . . . 45
7.1.4 Editing a program link (EDIT A-Z). . . . . . . . . . . . . . . . . . . . 46
7.1.5 Saving a program link (STO A-Z). . . . . . . . . . . . . . . . . . . . 47

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Table of contents
7.2 Centrifugation parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7.2.1 Temperature (t/°C). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
7.2.2 Start-up and run-down parameters. . . . . . . . . . . . . . . . . . 48
7.2.3 RCF and RAD. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.2.4 Speed (RPM). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
7.2.5 Runtime (t/hms). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
7.2.6 Precooling of the rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
7.3 Machine Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
7.3.1 Change Lock. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
7.3.2 Change PIN. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
7.3.3 Info. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
7.3.4 Operating Time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
7.3.5 Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
8 Cleaning, disinfection and maintenance. . . . . . . . . . . . . . . . . . . . 68
8.1 Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
8.2 Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
8.3 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
9 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
9.1 Performing a mains reset. . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
10.1 Type plate. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
10.2 Dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
11 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
12 Declaration of conformity and list of standards. . . . . . . . . . . . . . . 79
13 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
14 Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
15 Appendix. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
A Rotors and accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

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Introduction
Signal words
Warning categories
General symbols
1 Introduction
1.1 Symbols
Signal word Meaning
DANGER This combination of symbol and signal
word indicates an immediate dangerous
situation that will result in death or serious
injury if it is not avoided.
WARNING This combination of symbol and signal
word indicates a possible dangerous sit-
uation that can result in death or serious
injury if it is not avoided.
CAUTION This combination of symbol and signal
word indicates a possible dangerous sit-
uation that can result in minor injury if it is
not avoided.
NOTICE This combination of symbol and signal
word indicates a possible dangerous sit-
uation that can result in material and envi-
ronmental damage if it is not avoided.
Warning signs Type of danger
Warning – danger zone.
Warning – hand injuries.
Warning – high-voltage.
This listing symbol denotes descriptions of tasks that you must per-
form.
■This dot is for denoting lists.
Cross references are indicated as follows: ⮫ Chapter 1.1 ‘Symbols’
on page 5

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Introduction
Symbols on the device
Warnings/symbols on the device which are no longer rec-
ognizable should be immediately replaced by the operating
company.
The images shown in the following depict the positions of
the warnings and symbols afxed to the device.
Fig. 1: Warnings on the device
Attention, general danger point
Nonobservance of this warning can lead to material damage and personal
injury.
Before using the device, make sure you read the operating instructions and
observe the safety information.
Warning - Biological contamination!
Before using the device, make sure you read the operating instructions and
observe the safety information.
Warning - Hand injury
Nonobservance of this warning can lead to material damage and personal
injury.
Before using the device, make sure you read the operating instructions and
observe the safety information.

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Introduction
IOIOI
RS232
Fig. 2: Warning stickers on the right outer side
Attention, general danger point
Nonobservance of this warning can lead to material damage and personal
injury.
Before using the device, make sure you read the operating instructions and
observe the safety information.
Symbol for separate collection of electric and electronic devices
Symbol according to directive 2002/96/EC (WEEE). Applies in the countries
of the European Union, as well as in Norway and Switzerland.
Type plate
Nameplate with technical data specications.
Symbol for TÜV-inspected device
From TÜV-inspected device.
IOIOI
RS232
IOIOI
RS232
Fig. 3: Notice at the RS232 interface
Symbol for RS232 interface
This symbol indicates the RS232 interface (only for device with RS232 inter-
face).

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Introduction
1.2 Symbol on the shipping carton label
This way up.
Shows the correct upright position of the package.
Fragile, handle with care.
Refers to a medical device that can break or be damaged if handled carelessly.
temperature range
The shipping packaging must be stored, transported and handled within the temperature range
shown (-20°C - +60°C).
humidity range
The shipping packaging must be transported and handled within the indicated humidity range
(10% - 80%).
keep dry
The shipping packaging must be kept away from rain and kept in a dry environment.
1.3 Personal protective equipment
The assessment of the personal protective equipment must take place on
site according to the actual risks which are posed by the used substances,
the procedures and the environmental conditions.
The operating company must create a risk assessment according to the
corresponding standards and directives and enact work instructions which
ensure the safe handling of the device and accessories.
1.4 Personnel qualication
Repairs may only be performed by a person authorized by the manufac-
turer.

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Introduction
Skilled personnel are able to perform the work assigned to them and inde-
pendently recognize and avoid potential hazards based on their specialized
education (professionally recognized vocational training), knowledge and
experience as well as knowledge of the relevant regulations.
Personnel have participated in special instruction by the manufacturer of the
device or personnel authorized by the manufacturer and, through this, are
authorized to perform the corresponding task.
Personnel have completely read and understood this documentation.
A service technician is someone who, based on his professional training
and advanced practical knowledge, provides expert support (installation,
commissioning, maintenance, etc.) for our customers in all things mechan-
ical, on the phone as well as on site, and is an employee of the company of
the manufacturer or was trained and authorized by the manufacturer to
carry out service work on the device.
Intended use
Improper use
Interventions and modications to devices by persons not
authorized by the company Andreas Hettich GmbH & Co.
KG will be at one's own risk and result in the voiding of all
warranty claims, as well as the voiding of liability claims
against Andreas Hettich GmbH & Co. KG.
Authorized skilled personnel with special instruction
Service technician
1.5 Intended use
Dual centrifugation: The purpose of the ZentriMix is to process samples
pre-analytically in non-medical laboratories.
This includes the extraction, grinding, mixing and dissolving as well as
homogenizing of samples, so also the decomposition of organic or inor-
ganic materials.
Another purpose is the creation of nanoparticles through homogenization
(e.g. lipid nanoparticles, particularly liposomes) for non-clinical uses.
Classical centrifugation: In its function as a classical centrifuge, it is used for
the separation of materials or material mixtures with a density of max. 1.2
kg/dm³ in non-medical laboratories.
The device was not designed for use in environmental conditions that do
not meet the manufacturer requirements or in a potentially explosive, radio-
active or biologically or chemically contaminated atmosphere.
The ZentriMix is only meant for the above-named purposes. Another use or
one which goes beyond this is considered to be improper. The manufac-
turer is not liable for damage resulting from this.
Intended use also includes the observation of all information in the oper-
ating instructions and complying with the inspection and maintenance inter-
vals.
The device was not designed for use under environmental conditions that
do not meet the manufacturer requirements or in a potentially explosive,
radioactive or biologically or chemically contaminated atmosphere.
The device is not meant for processing toxic, corrosive or infectious sam-
ples; such samples must not be processed in the ZentriMix.

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Introduction
Foreseeable misuse The device is built according to the state-of-the-art and the recognized
safety regulations. If used and handled improperly, there could be life-
threatening danger to the user or third parties, or the device could be
impaired or there could be other property damage. The device is only to be
used for its intended purpose and only when it is in safe working condition.
Malfunctions which could affect safety must be corrected immediately.
If consumable material approved/sold by the manufacturer is not used,
there is a danger of container damage at high speeds, which could result in
a spontaneously high unbalance and damage to the device.
Do not operate the device unsupervised.
If used improperly, incorrect input of the parameters can result in dangerous
operating conditions: For example, if the duration of a run is entered too
high, the sample could overheat, which could cause the sample container
to burst, which could result in extreme unbalance at high speed and
damage to the device.
1.6 Replacement parts/consumable material
Only original replacement parts and approved original accessories of the
manufacturer may be used and only original consumable material or that
recommended (approved) by the manufacturer in its ofcial documentation
may be used.
WARNING
If original replacement parts, consumable material approved
or sold by the manufacturer and original accessories are not
used, any warranty or liability claims against the company
Andreas Hettich GmbH & Co. KG are void.
1.7 Scope of delivery
■1x device
■1 x mains cable (power supply)
■1 x size 5 Allen wrench (for emergency release and mounting/
dismounting the rotor)
■1 x lubricating grease for lifting lugs
■1x Allen wrench, size 2.5
■1 x Allen keys, short, size T20 SG
■1 x operating instructions
Rotor(s) and the corresponding accessories are included in
delivery, depending on the order.
1.8 Return shipments
If the device or its accessories are sent to the manufacturer, this/these must
be decontaminated and cleaned before shipping in order to protect per-
sonnel, the environment and material. For returns, please contact Hettich or
the responsible Hettich sales partner. You will then receive a clearance cer-

11 / 88
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Introduction
ticate and return material authorization number. Devices without a signed
clearance certicate will not be accepted in order to protect our employees.
We ask for your understanding.

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Safety information
Danger due to electrical
energy
Danger due to kinetic energy
Danger due to insufficient
maintenance or maintenance
not performed in a timely
manner
2 Safety information
The device and its associated components and assemblies
meet the currently valid safety standards individually and as
a whole and meet the CE directives of the European Union.
The device is safe if used as intended and observing the
descriptions and information given in this documentation.
WARNING
Warning of incorrect voltage on the device protection switch!
Pay attention to the correct operating voltage in order to
prevent damage to the device.
−Only operate the device at the correct operating voltage.
−Refer to the technical data and nameplate for the value
for the correct operating voltage.
−If the operating voltage is too high, the overcurrent pro-
tection fuse in the device switch can trip and the device
electronics can be destroyed.
DANGER
Danger due to rotor moving with high kinetic energy!
There is a risk of injury if the lid is opened via the emergency
release during device operation.
−Only open the device via the emergency release when
the device is switched off.
−Before the emergency release, make sure that the
device rotor has stopped.
DANGER
Danger due to insufcient maintenance or maintenance not
performed in a timely manner!
There is a risk of collision inside the device due to insufcient
maintenance or maintenance not performed in a timely
manner.
−Have maintenance done at the specied intervals.
−Check the rotor for a rm t before using the device.
Correctly tighten the fastening screw. If the rotor has
play, it must be replaced.
−Check the device for visible damage or deciencies
before use. If you discover deciencies or damage, put
the device out of operation and immediately inform an
authorized service technician.

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Safety information
Warning of premature pro-
gram abort
Warning of hand injury due to
rotor gears
Risk of injury due to the
device falling out of the pack-
aging
Warning of hand injury due to
device lid falling closed
unbraked
WARNING
Warning of premature program abort!
In the event of a premature program abort, such as a power
failure, switching off during the program run or pulling out
the mains plug, the desired effect on the samples might not
be achieved.
−Do not switch off the device during a program run.
−Do not actuate the emergency release during a program
run.
−Do not pull out the mains plug during a program run.
−After a program abort, check whether the samples can
be used.
CAUTION
Warning of hand injury due to rotor gears!
There is a risk of injury to ngers by the rotor gears if this is
turning. Cuts with possible infections could result.
−Do not grab between the gears of the rotating units and
the rotor.
−Avoid rotor rotation with the lid open as much as pos-
sible.
−To prevent injuries to third parties, do not leave the
device unsupervised with the lid open.
WARNING
Risk of injury due to parts falling out of the packaging!
When parts fall out of the transport packaging, there is a risk
of crushing injuries to extremities.
−Observe the warnings for opening the packaging.
−Only open the packaging at the specied point.
WARNING
Warning of hand injury due to device lid falling closed
unbraked!
If the lid falls closed unbraked, there is a risk of extremities
getting caught.
−Have the device maintained by a service technician at
the specied intervals.
−Always open the device lid completely.
−If a gas pressure spring of the lid is defective, have it
replaced by a service technician.

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Safety information
Danger posed by selection of
unsuitable parameters for dual
centrifugation
Damage due to incorrect
loading for dual centrifugation
WARNING
Danger posed by selection of unsuitable parameters for dual
centrifugation!
The maximum permissible speed (RPM) for ZentriMix appli-
cations depends on the sample composition and sample
container. Every ZentriMix sample container can only be
used up to a certain rotational speed depending on the
design and application; this must not be exceeded under
any circumstances! Otherwise, there is a danger that the
sample container will not withstand the stress, and the
device will be damaged due to the resulting unbalance; the
heat that arises cannot be compensated by the cooling
system, resulting in the sample container becoming very hot.
Only remove such a container after a sufcient cooling-off
time while wearing appropriate protective clothing.
−ZentriMix applications are to be carried out according to
the protocol authorized by the manufacturer.
−Every application not yet described by a manufacturer
protocol must be checked and validated for any hazard
potential before its use. ⮫ Further information
on page 36
.
−The correct parameter input must always be checked.
−Do not leave the device unsupervised.
WARNING
Damage due to incorrect loading for dual centrifugation!
The two rotor receptacles must be loaded strictly symmetri-
cally with the same weight.
−Observe the loading rules. ⮫
Chapter 6.5.2 ‘Loading a
rotor for dual centrifugation’ on page 33 !
−Observe the device as it starts up. If unusually high
unbalance occurs, stop the run immediately by pressing
the [Stop/Open] key twice and check the load. If you
cannot determine that the load is incorrect, put the
device out of operation and inform the Service depart-
ment.

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Safety information
Danger due to hazardous sub-
stances in the sample
Explosion and fire hazard
Danger due to the penetration
of water or other liquids
DANGER
Danger due to hazardous substances in the sample
No materials or material mixtures may be processed in a
way which poses a risk of re or explosion.
−Therefore, thoroughly check the intended process
regarding this hazard and take the appropriate measures
to avoid personal injury.
−Follow the relevant regulations and directives for han-
dling chemicals and hazardous substances.
−In particular, no aggressive chemicals may be used (e.g.
dangerous, corrosive extracting agents, such as chloro-
form, strong acids).
WARNING
Explosion and re hazard
Do not process any explosive materials or materials which
can react together chemically with high energy. Substances
which could create a hazardous explosive or ammable
atmosphere in the device may not be processed. The esti-
mation of such risks is the responsibility of the operating
company. The operating company must instruct the user
with regard to this.
−Therefore, thoroughly check the intended process
regarding these hazards and take the appropriate meas-
ures to prevent personal injury.
−Follow the relevant regulations and directives for han-
dling chemicals and hazardous substances.
−In particular, no aggressive chemicals may be used (e.g.
dangerous, corrosive extracting agents, such as chloro-
form, strong acids).
DANGER
Danger due to the penetration of water or other liquids!
When water penetrates into the device, there is a danger of
a short circuit and the user suffering an electric shock.
−Prevent water and moisture from penetrating into the
device.
−Do not pour liquids into the device interior.
−The device must not stand in the rain.
−Transport is only allowed with suitable transport protec-
tion.

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Safety information
Warning - Impermissible
ambient temperature
Warning - Position changed
due to vibration
Mechanical emergency
release
WARNING
Warning - Impermissible ambient temperature!
If the ambient temperature is outside of the permissible
ambient temperature range for the samples, these might be
compromised.
−Observe the permissible maximum and minimum
ambient temperatures for setting up the device.
−Do not place the device next to heat sources or devices
which radiate heat.
−Check the samples after any device overheating to see if
they can still be used and take new samples, if neces-
sary.
−Make sure the device is not exposed to direct sunlight.
−Make sure the device is not exposed to frost.
−Maintain the minimum clearance around the device.
−Make sure that the fan is not blocked.
−Never operate the device in a continuous run.
WARNING
Warning - Position changed due to vibration!
The device might fall down if the set-up surface vibrates or if
it is not at or stable enough.
−Only place the device on a stable, at surface.
−The set-up surface must reliably bear the weight of the
device.
WARNING
There is a risk of injury if the mechanical emergency release
of the device is actuated during the program run.
If the mechanical emergency release of the lid is actuated
during a program run, the program is aborted and the rotor
runs down unbraked.
−Only actuate the emergency release when the rotor is
stopped.
−Check whether the rotor has stopped before an emer-
gency release.

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Safety information
Danger of infections due to
improper disposal DANGER
Danger of infections due to improper disposal!
In the event of improper disposal of samples, there is a risk
of injury to the user, disposal personnel and the environ-
ment.
−Observe the legal regulations for disposing of contami-
nated material.

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Device description
12
Fig. 4: Operating elements, front
side
1 Control panel
2 Lid
12
Fig. 5: Device interior
1 Centrifuging chamber
2 Rotor cavity
1 2
Fig. 6: Right outer side
1 Device plug (power supply)
2 Device switch [On/Off]
3 Device description
Further information about the control panel can be found in
the Operation chapter ⮫ Chapter 6 ‘Operation’
on page 27.

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Device description
1
Fig. 7: Rear side
1 RS232 interface

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Transport and storage
4 Transport and storage
Dimensions and weight with transport packaging
80
100
ca.925
ca.620
ca.630
Fig. 8: Transport packaging dimensions
Data Value Unit
Outer dimensions (L x W x H) 925 x 620 x
630
mm
Weight approx. 105 kg