Varian 900-LC Series Parts list manual

Varian 900-LC Series
Liquid Chromatograph
Getting Started Manual
Installation Category II
Pollution Degree 2
Safety Class 1 (EN 61010-1)
8510243600
December 2007

Varian 900-LC Series Liquid Chromatograph
Varian, Inc. offices
Varian, Inc. has offices in most countries. The major offices for liquid
chromatography products are listed below:
Varian Australia Pty Ltd (Manufacturing site)
679 Springvale Road
Mulgrave, Victoria 3170
Australia
International telephone: + 61 3 9560 7133
International fax: + 61 3 9560 7950
Varian Instruments
2700 Mitchell Dr
Walnut Creek, CA 94598
Telephone: [phone]
International telephone: [phone]
International fax: [phone]
Varian BV
Herculesweg 8
4338 PL, Middelburg, Netherlands
International telephone: [phone]
International fax: [phone]
Internet
The Varian, Inc. Internet home page can be found at:
www.varianinc.com
Varian Australia Pty Ltd is the owner of copyright on this document
and any associated software. Under law, the written permission of
Varian Australia Pty Ltd must be obtained before either the
documentation or the software is copied, reproduced, translated or
converted to electronic or other machine-readable form, in whole, or
in part.
First published December 2007 in Australia. Comments about this
manual should be directed to the Marketing Communications
Manager, Varian Australia at the address above or by e-mail to:
[email protected]
Varian Australia is ISO9001:2000 certified.
©2007 Varian Australia Pty Ltd (ABN 67 004 559 540)
Galaxie, Varian and the Varian logo are trademarks or registered
trademarks of Varian, in the U.S. and other countries.
All rights reserved.
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Varian 900-LC Series Liquid Chromatograph
Declaration of Conformity
We hereby declare that the equipment listed below complies with the requirements of:
The Low Voltage Directive 73/23/EEC (93/68/EEC)
The EMC Directive 89/336/EEC (92/31/EEC and 93/68/EEC)
Applicable Standards
LVD BS EN 61010-1:1993
EMC BS EN 61326:2003 EN 55011:1999 EN 61000-4-2:1995
EN 61000-4-3:2003 EN 61000-4-4:1995 EN 61000-4-5:1995
EN 61000-4-6:1996
Equipment Model Number Varian Model Instrument Type
Authorized Representative in the EU
Name: G. A. Wassink Company Name Varian BV
Signed:
Address Herculesweg 8
4330 EA Middelburg
The Netherlands
Position: Quality Manager Telephone [phone]
Date: 1 Month 20XX Facsimile [phone]
Manufacturer
Name: Phil Binns Company Name Varian Australia Pty Ltd
Signed:
Address 679 Springvale Road
Mulgrave VIC 3170
Australia
Position: Director of Operations Telephone [phone]
Date: 1 Month 20XX Facsimile [phone]
Publication date: December 2007 3
Publication number 8510243600 Month 20XX

Varian 900-LC Series Liquid Chromatograph
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Varian 900-LC Series Liquid Chromatograph
Contents
Safety Practices and Hazards................................................................9
General 9
Electrical Hazards 10
Compressed Gas Cylinders 11
High Pressure Hazards 12
Ultraviolet Radiation 13
Flash Chromatography 13
Solvent Hazards 13
Ignition of Flammable Chemicals 14
Inflammation or Injury due to Toxic, Corrosive or Stimulative
Solvent 14
Explosion of Vapor from Flammable Chemicals 14
Other Precautions 15
Warning and Caution Messages 15
Warning Symbols 16
Color Coding 17
US FCC Advisory Statement 17
Spurious and Harmonic Radiation Compliance 18
CE Compliance 18
1. Introduction ................................................................................ 19
1.1 Pre-installation Requirements 19
1.2 User Documentation 19
1.2.1 Conventions 19
1.2.2 Notes and Hot Tips 20
1.3 Specifications 20
1.3.1 Environmental Conditions 20
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Varian 900-LC Series Liquid Chromatograph
1.3.2 Electrical Power Supplies 21
1.3.3 Gas Supplies 23
1.3.4 Waste Container 24
1.3.5 Laboratory Conditions 24
1.3.6 Instrument Cooling Air Supply 24
1.3.7 Weights and Dimensions 25
1.4 Personal Computer Requirements 26
2. Installation .................................................................................. 29
2.1 900-LC Series LC System to PC Communications 29
2.2 Installing the Galaxie™ Chromatography Data System 32
2.3 About the Varian Integrated HPLC Online Help 40
2.4 Hardware Installation 41
2.5 Installation Qualification Tests 41
2.6 Customer Training 41
2.7 Using the Online Help 42
2.8 Instrument Maintenance Software (IMS) 42
2.8.1 How to launch IMS 42
2.8.2 About IMS 43
3. Operation..................................................................................... 45
3.1 Analysis Checklist 45
3.2 Turning on the Instrument and Software 45
3.2.1 Connecting to a System 46
3.3 Preparing the System for Use 46
3.4 Configuring the Device Settings and System Parameters 47
3.4.1 Flows 48
3.4.2 Detectors 48
3.4.3 Column 48
3.4.4 Sample Introduction 49
3.4.5 System Parameters 49
3.5 Creating a Method 50
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Varian 900-LC Series Liquid Chromatograph
3.6 Starting an Acquisition 52
3.6.1 Sequence 52
3.6.2 Quick Start 52
3.7 Printing a report 53
3.8 Saving Your Files 54
4. Maintenance................................................................................ 55
4.1 Routine Maintenance 56
4.2 Cleaning 57
4.3 Spare Parts 57
5. Troubleshooting .......................................................................... 59
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Varian 900-LC Series Liquid Chromatograph
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Varian 900-LC Series Liquid Chromatograph
Safety Practices and Hazards
Your Varian instrument and accessories have been carefully
designed so that when used properly you have an accurate, fast,
flexible and safe LC system.
Information about safety practices appears throughout the
documentation (both hard copy and online) provided with your
instrument. Before using the instrument, you must thoroughly read
these safety practices.
Observe all relevant safety practices at all times.
This instrument is designed for chromatographic analysis of
appropriately prepared samples. It must be operated using
appropriate solvents and within specified maximum ranges for
pressure, flows, and temperatures as described in this manual. If
the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.
It is your responsibility to inform Varian representatives if the
instrument has been used for the analysis of hazardous biological,
radioactive, or toxic samples, prior to any instrument service being
performed or when an instrument is being returned to the service
center for repair, or is being recycled at end-of-life.
General
Operation of a Varian 900-LC Series Liquid Chromatograph
involves the use of pressurized liquid, ultraviolet radiation, and
hazardous materials including corrosive fluids and flammable
liquids. Careless, improper or unskilled use of this liquid
chromatograph can cause death or serious injury to personnel,
and/or severe damage to equipment and property.
The 900-LC Series Liquid Chromatograph incorporates covers that
are designed to prevent inadvertent contact with any potential
hazards. If the instrument is used in any manner not specified by
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Varian 900-LC Series Liquid Chromatograph
Varian, this protection may be impaired. It is essential that no
cover is bypassed, damaged or removed.
The safety practices described below are provided to help the user
operate the instrument safely. Read each safety topic thoroughly
before attempting to operate the instrument and ALWAYS operate
the liquid chromatograph in accordance with these safety
practices.
Follow these safety practices to ensure safe equipment operation.
•Perform periodic leak checks on all supply lines and
pneumatic plumbing.
•Do not allow liquid lines to become kinked or punctured. Place
lines away from foot traffic and extreme heat or cold.
•Store organic solvents in fireproof, vented and clearly labeled
cabinets so they are easily identified as toxic and/or
flammable materials.
•Keep the waste vessel in view and empty as required.
•Do not accumulate waste solvents. Dispose of such materials
through a regulated disposal program and not through
municipal sewage lines.
This instrument has been tested per applicable requirements of
EMC Directive as required to carry the European Union CE Mark.
This equipment may be susceptible to radiation/interference levels
or frequencies, which are not within the tested limits.
Electrical Hazards
Exposure to high voltages and UV energy can cause severe skin
damage and cataracts of the eyes, while close contact with the
electrical components can result in severe heat burns to the skin,
and an electrical discharge which may cause death, severe electric
shock or sub-surface skin burns.
The 900-LC Series Liquid Chromatograph has been carefully
designed to operate safely and effectively when using components
that conform to Varian’s design criteria. Use of non-approved
components in the LC system may render the system inoperative
and/or hazardous. It may also invalidate the warranty on the
instrument. Use only related components supplied or authorized by
Varian.
•Disconnect the instrument from all power sources before
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Varian 900-LC Series Liquid Chromatograph
removing protective panels to avoid exposure to potentially
dangerous voltages. Panels or covers which are retained by
screws on the LC system may be opened ONLY by Varian-
trained, Varian-qualified, or Varian-approved Representatives.
Consult the manuals or product labels supplied with your PC
to determine which parts are operator-accessible.
•When it is necessary to use a non-original power cord plug,
make sure the replacement cord adheres to the color coding
and polarity described in the manual and all local building
safety codes.
•Good grounding/earthing is essential to avoid a potentially
serious electric shock hazard. Consult the manuals or product
labels supplied with your PC for the relevant grounding
requirements.
•Replace blown fuses with fuses of the size and rating shown
on the fuse panel or in the manual.
•Replace faulty or frayed power cords immediately with the
same type and rating.
•Make sure that voltage sources and line voltage match the
value for which the instrument is wired.
•Avoid using power supplies from a source that may be subject
to electrical or RF interface from other services (for example,
large electrical motors, elevators and welders).
Compressed Gas Cylinders
Unless your system is equipped with a helium sparging unit or an
ELS detector, there are no gas requirements for the 900-LC Series
LC systems. For gas requirements on these devices, consult the
Operation Manual or other documentation enclosed with the
particular device. Compressed gas cylinders contain highly
pressurized gas. If storage conditions are outside of the
recommended suppliers safety codes the cylinders can explode or
rapidly release gas into the environment. This may result in injury
or death.
•Store and handle compressed gases carefully and in strict
adherence to safety codes.
•Secure cylinders to an immovable structure or wall.
•Store and move cylinders in an upright, vertical position.
Before transport, remove regulators and install cylinder cap.
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Varian 900-LC Series Liquid Chromatograph
•Store cylinders in a well ventilated area away from heat, direct
sunshine, freezing temperatures, and ignition sources.
•Mark cylinders clearly so there is no doubt as to their
contents.
•Use only approved regulators and connections.
•Use only connector tubing that is chromatographically clean
(Varian part number 0391832600) and has a pressure rating
significantly greater than the highest outlet pressure from the
regulator.
Storage Cylinder Instructions
Cylinders containing gas under pressure should be firmly secured
to a rigid structure, and the storage area must be adequately
ventilated.
Never locate gas cylinders near a source of ignition, or in a
position that is subject to direct heat. Gas storage cylinders often
incorporate a pressure release device which will discharge the gas
at a predetermined temperature, usually around 52 °C (125 °F).
If gases are to be plumbed from a remote storage area to the
instrument site, ensure that the local outlets are fitted with stop
valves, pressure gauges and suitable regulators which are easily
accessible to the instrument operator. The gas outlets must be
provided within 1.5 metres (5 feet) of the instrument
High Pressure Hazards
If a line ruptures, a relief device opens, or a valve opens
accidentally under pressure, potentially hazardous high liquid
pressures can be generated by the pump causing a high velocity
stream of volatile and/or toxic liquids.
•Wear personal protective equipment when you inject samples
or perform routine maintenance.
•Never open a solvent line or valve under pressure. Stop the
pump first and let the pressure drop to zero.
•Read and adhere to all NOTES, CAUTIONS, and WARNINGS
in the manual.
•Always keep the doors and covers closed during operation.
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Varian 900-LC Series Liquid Chromatograph
Ultraviolet Radiation
900-LC Series Liquid Chromatograph systems that use an
ultraviolet light source have shielding to prevent radiation exposure
to personnel.
For continued protection:
•Ensure that any protective lamp covers are in place during
operation.
•Do not look directly into detector fluid cells or at the UV light
source. When inspecting the light source or fluid cell, always
use protective eye covering such as borosilicate glass or
polystyrene.
•Ozone can be generated by radiation from source lamps.
Exposure to ozone can result in severe irritation to the skin,
eyes, and upper respiratory system. The maximum
permissible exposure level is 0.1 ppm (0.2 mg/m3). ALWAYS
ventilate the area surrounding the LC system such that the
concentration of ozone does not exceed the maximum
permissible level. All venting must be to outside air, never
within the building.
Flash Chromatography
The operator should be familiar with the physico-chemical
properties of the components of the mobile phase.
Only use solvents compatible with the LC system tubing and
fittings as certain solvents may cause weakening and leaks with
possible bursting.
Employ static measuring and static discharge devices to safeguard
against the buildup of static electricity.
Solvent Hazards
All solvents can create a hazard if they leak into the atmosphere.
Even small leaks in solvent supply systems can be dangerous. Any
leak (except that of air) can result in an oxygen-deficient
atmosphere, which can cause asphyxiation. The area in which
solvents are stored and the area surrounding the instrument must
be adequately ventilated to prevent such accumulations.
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Varian 900-LC Series Liquid Chromatograph
Ignition of Flammable Chemicals
This instrument is not explosion-proof. In unattended operation, do
not use organic solvents having an ignition point below 70 °C.
Beware of ignition hazard when using flammable chemicals such
as organic solvents.
Do not bring a heat or flame source near the instrument.
Well-ventilate the laboratory room where the instrument is used.
Always check the following conditions. If an abnormality is found
stop operation immediately.
•Leakage of solvent or waste solution.
•Leakage of solvent inside the instrument.
When using flammable chemicals, be careful about possible
ignition due to static electricity. To prevent the build-up of static
electricity, use a conductive container for waste.
Inflammation or Injury due to Toxic, Corrosive or
Stimulative Solvent
When using a toxic, corrosive or stimulative solvent, be careful not
to incur a physical inflammation or injury. For details of the
properties of each solvent and how to handle it, refer to the
relevant Material Safety Data Sheets (MSDS). Be sure to handle
each solvent properly.
Wear proper protective clothes (e.g., safety goggles) so that a
solvent will not come into direct contact with the skin.
Ventilate the laboratory room adequately to prevent accidental
inhalation of harmful solvent vapor.
Explosion of Vapor from Flammable Chemicals
If a flammable chemical such as organic solvent leaks from the
flow path of the instrument and its vapor concentration reaches the
explosion limit, it could cause spontaneous combustion with
dangerously explosive results.
When using a flammable and readily volatile chemical, be sure to
check for leakage from the instrument flow path and ventilate the
laboratory room adequately.
Use only approved regulator and hose connectors (refer to the
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Varian 900-LC Series Liquid Chromatograph
supplier’s instructions). Keep solvents cool and properly labeled.
Ensure that you have the correct solvent before connecting it to the
instrument.
Other Precautions
Use of the 900-LC Series Liquid Chromatograph and accessories
may involve materials, solvents and solutions, which are
flammable, corrosive, toxic or otherwise hazardous. Careless,
improper or unskilled use of such materials, solvents and solutions
can create explosion hazards, fire hazards, toxicity and other
hazards that can result in death, serious personal injury or damage
to equipment.
Always ensure that laboratory safety practices governing the use,
handling and disposal of hazardous materials are strictly observed.
These safety practices should include wearing appropriate safety
clothing and safety glasses.
Airflow to the cooling fans of the liquid chromatograph must be
unobstructed. Do not block the ventilation grills on the liquid
chromatograph and accessories. Consult the manuals supplied
with your PC, monitor and for their specific ventilation
requirements.
Great care should be taken when working with glass or quartz
parts to prevent breakage and cuts.
The 900-LC Series Liquid Chromatograph weighs approximately
140 kg (309 lb). To avoid injury to personnel or damage to the
instrument or property, always use appropriate lifting procedures to
move the instrument.
Use only Varian-supplied spares with your instrument.
Warning and Caution Messages
A Warning message is used in the text when failure to observe
instructions or precautions could result in death or injury. The list of
symbols that appear in conjunction with warnings are detailed in
the next section.
A Caution message is used when failure to observe instructions
could result in damage to equipment (Varian supplied and/or other
associated equipment).
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Varian 900-LC Series Liquid Chromatograph
Warning Symbols
The following is a list of symbols that appear in conjunction with
warnings in this manual or on the 900-LC Series Liquid
Chromatograph. The hazard they describe is also shown.
A triangular symbol indicates a warning. The meanings of the
symbols that may appear alongside warnings in the documentation
or on the instrument itself are as follows:
Corrosive liquid Electrical shock Explosion hazard
Eye hazard
Fire hazard
Heavy weight
(danger to feet)
Heavy weight
(danger to hands)
Hot surface
Moving parts
Radiation source
The following symbol may be used on warning labels attached to
the instrument. When you see this symbol, refer to the relevant
operation or service manual for the correct procedure referred to
by that warning label.
The following symbols appear on the instrument for your
information.
I Mains power on
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Varian 900-LC Series Liquid Chromatograph
0 Mains power off
Fuse
Single phase alternating current
Direct current
When attached to the rear of the instrument,
indicates that the product complies with the
requirements of one or more EU directives.
When attached to the rear of the product, indicates
that the product has been certified (evaluated) to
CSA 1010.1 and UL 3101-1.
Color Coding
The various indicator lights appearing on Varian instruments and
associated accessories are color coded to represent the status of
the instrument or accessory.
•A green light indicates the instrument is in normal/standby
mode.
•An orange light indicates that a potential hazard is present.
•A blue light indicates that operator intervention is required.
•A red light warns of danger or an emergency.
US FCC Advisory Statement
This equipment generates, uses and can radiate radio frequency
energy, and if not installed and operated in accordance with the
instruction manual may cause interference to radio
communications. It has been tested and found to comply with the
limits for a Class A computing device pursuant to Subpart J of Part
15 of Federal Communications Commission (FCC) Rules, which
are designed to provide reasonable protection against such
interference when operated in a commercial environment.
Operation of this equipment in a residential area may cause
interference, in which case the user will be required to take
whatever measures may be necessary to correct the interference
at his or her expense.
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Varian 900-LC Series Liquid Chromatograph
Spurious and Harmonic Radiation Compliance
Your Varian 900-LC Series Liquid Chromatograph complies with
the requirements of FCC rules and Regulations part 18, subpart
H—Miscellaneous Equipment, at the date of manufacture.
CE Compliance
Your Varian 900-LC Series Liquid Chromatograph has been
designed to comply with the requirements of the Electromagnetic
Compatibility (EMC) Directive and the Low Voltage (electrical
safety) Directive (commonly referred to as the LVD) of the
European Union. Varian has confirmed that each product complies
with the relevant Directives by testing a prototype against the
prescribed EN (European Norm) standards.
Proof that a product complies with these directives is indicated by:
•The CE Marking appearing on the rear of the product, and
•The documentation package that accompanies the product
containing a copy of the Declaration of Conformity. The
Declaration of Conformity is the legal declaration by Varian
that the product complies with the directives listed above, and
shows the EN standards to which the product was tested to
demonstrate compliance. It is also signed by Varian’s
Authorized Representative in the EU, and by the
representative of the manufacturing plant.
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Varian 900-LC Series Liquid Chromatograph
1. Introduction
1.1 Pre-installation Requirements
Prior to receiving your instrument, you will have been provided with
a 900-LC Series Liquid Chromatograph Pre-installation Manual
(publication number 8510243900), which describes the
environmental and operating requirements of the LC system. You
must prepare your laboratory according to these instructions
before the LC system can be installed. You should keep the pre-
installation manual for future reference. If you have misplaced your
copy, you can obtain a free replacement from your local Varian
office. Alternatively, download a PDF from the Varian, Inc. Web
site, www.varianinc.com
1.2 User Documentation
You have been provided with the following documentation to help
you set up and operate your Varian Integrated Liquid
Chromatograph:
•This Getting Started manual, with safety practices and hazards
information, where to find information about installing and
maintaining instrument components and a brief operation
overview.
•An extensive online Help system containing context-sensitive
help, step-by-step instructions for frequently performed
operations and instructions for using any accessories you ordered.
1.2.1 Conventions
The following conventions have been used throughout the
documentation:
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Varian 900-LC Series Liquid Chromatograph
•Menus, menu options and field names (e.g. select Copy from the
Edit menu) have been typed in bold. Bold is also used to signify
the pushbuttons appearing throughout the software (e.g. click OK).
•ALL CAPITALS indicate keyboard and mouse commands (e.g.
press the F2 key) and text you must type in from the keyboard
(e.g. type SETUP at the prompt).
1.2.2 Notes and Hot Tips
A Note is used to give advice or information. A Note is denoted by
the following symbol: ✒
A Hot Tip is used to give practical hints to help you achieve the
best possible performance from your instrument. A Hot Tip is
denoted by the following symbol: ✰✰✰
1.3 Specifications
The instrument is suitable for indoor use only and is classified
suitable under the following categories (EN 61010-1):
Installation category II
Pollution degree 2
Safety class 1
1.3.1 Environmental Conditions
Condition Altitude Temp
(°C)
Humidity (%RH)
non-condensing
Non-operating
(Transport)
0–3 050 m
(0–10 000 ft)
5–45 20–80
Non-operating &
meeting dielectric
strength tests
Sea level 40 90–95
Operating within
specifications
0–2 000 m
(0–6 560 ft)
10–35 8–80
Table 1. Environmental conditions
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