ChemoMetec NucleoCounter YC-100 User manual

NucleoCounterYC-100
User's Guide
Revision 1.3
Technology that counts

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NucleoCounterYC-100
Yeast Cell Counter
P/N 991-0300 (English)
Revis ion 1. 2
Decem ber, 2005
ChemoMetec A/S
Gydevang 43, DK-3450 Allerød, Denmark
Telephone: [phone], Fax: [phone]
Internet: www.chemometec.com,
E-mail: [e-mail]

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Caution!
This equipment must be operated as described in this User’s Guide. Please read the entire guide
before attempting to use this unit. Please pay attention to that gloves or protective clothing are not
worn on the illustrations/pictures shown in this User’s Guide. However, ChemoMetec A/S does
recommend that the user wear suitable protective clothing etc.
Contacting support
Technical information including product literature, answers to questions regarding the operation of the
NucleoCounterYC-100™ not covered in this document and information on software upgrade is
available through the following:
For e-mail support, send questions to NucleoCounterYC-100™ Technical Support on the
address [e-mail]
To speak with a Technical Support Specialist, call ([phone].
Please note the NucleoCounterYC-100™ serial number and have it available when contacting
ChemoMetec A/S for support. The NucleoCounterYC-100™ serial number is found on the label
affixed to the bottom of the instrument and in the display upon start-up.
Sales and ordering information
For sales assistance with NucleoCounterYC-100™ or the NucleoViewsoftware, to place an order
for a NucleoCounterYC-100™ or consumables, call ([phone], fax [phone], or send
e-mail to [e-mail]
Disclaimer Notices
The material in this document is for information only and is subject to change without notice. While
reasonable efforts have been made in the preparation of this document to assure its accuracy,
ChemoMetec A/S assumes no liability resulting from errors or omissions in this document, or from the
use of the information contained herein.
ChemoMetec A/S reserves the right to make changes in the product design without reservation and
without notification to its users.
Copyright Notices
Copyright © ChemoMetec A/S 2005. All rights reserved. No part of this publication may be reproduced,
stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical,
photocopying, recording or otherwise, without the prior written consent of ChemoMetec A/S, Gydevang
43, DK-3450 Allerød, Denmark.
ChemoMetec and NucleoCounter are registered trademarks owned by ChemoMetec A/S.
NucleoCounter, NucleoCassettes, Reagent Y100 and NucleoView are trademarks of ChemoMetec A/S.
All other trademarks are the property of their respective owners.

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Declaration of Conformity
Name of product: NucleoCounter
Type: YC-100
Other identifying data: Part no. 900-0300
The product complies with requirements of the following directives:
89/336/EEC - Electromagnetic Compatibility (EMC)
89/392/EEC - Machinery Directive
Harmonized standards, which have been used:
EN61326: 1997+A1: 1998 (Class B) +A2: 2000, Electrical equipment for
measurement, control and laboratory use –EMC requirements (emission and
immunity). Annex B and C from A1: 1998 is used.
This device complies with Part 15 of the FCC Rules. Operation is subject to the
following two conditions: (1) this device may not cause harmful interference, and (2)
this device must accept any interference received, including interference that may
cause undesired operation.
Date: 2005-11-04 Signed:
Name: Frans Ravn
Position: Chief Technology Officer
Name and address of manufacturer:
ChemoMetec A/S
Gydevang 43
DK-3450 Allerod
DENMARK

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WEEE directive information –Europe only
Correct Disposal of This Product
(Waste Electrical & Electronic Equipment) - Europe only
This marking shown on the product or its literature, indicates that it
should not be disposed together with other household wastes at the
end of its working life. To prevent possible harm to the environment or
human health from uncontrolled waste disposal, please separate this
from other types of wastes and recycle it responsibly to promote the
sustainable reuse of material resources.
Business users should contact their supplier and check the terms and
conditions of the purchase contract. This product should not be mixed
with other commercial wastes for disposal.
This information is listed in “Appendix 1: WEEE directive information in
more EU languages”.

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Introduction and intended use
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Introduction and intended use
The NucleoCounter YC-100 is intended to be used for the determination of yeast
cell concentration in sample containing dispersed yeast cells in suspension.
The NucleoCounter YC-100 has been developed
to count dispersed yeast cells in suspension
The instrument is a part of the NucleoCounter YC-100 system, which also comprises
a disposable NucleoCassette and a lysis buffer (Reagent Y100).
The NucleoCounter YC-100 system allows reliable, fast and objective cell counting
to be carried out based on an automated method of fluorescence microscopy. The
system enables the user to stain and count cells with good protection against being
exposed to potentially hazardous DNA staining dyes.
The NucleoCounter YC-100 is developed as a stand-alone instrument but optionally
it can be connected to a computer via an USB-interface. When connected to a
computer the NucleoView software offers various features such as documentation of
the obtained results. For further description of the software please refer to the
NucleoView User’s Guide.
The NucleoCounter YC-100 has the ChemoMetec part number 900-0300. Refer to
section 10.1 with respect to other part numbers of the items of the NucleoCounter
YC-100 system.
The NucleoCounter YC-100 system is not intended for human or veterinary
diagnostic purposes.

Introduction and intended use
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Warnings and precautions
Wherever the symbol appears on the NucleoCounter instrument, it indicates that
the manual must be consulted for precautions and warnings.
Power and cables
Use the shielded USB cable supplied with the NucleoCounter to ensure that you
maintain the appropriate EMI classification for the intended environment.
The USB interface connector of the NucleoCounter must only be connected to
SELV circuits. External computing devices connected to the USB interface
connector of the NucleoCounter has to comply with the standards, UL 1950 and
IEC/EN 60950.
Table 1.Description of the NucleoCounter USB interface connector
Use the shielded printer cable supplied with the NucleoCounter to ensure that you
maintain the appropriate EMI classification for the intended environment.
The printer interface connector of the NucleoCounter must only be connected to
the printer supplied by ChemoMetec A/S. External computing devices connected to
the printer interface connector of the NucleoCounter has to comply with the
standards, UL 1950 and IEC/EN 60950.
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In normal operation mode (refers to Pin no 4)
Pin no.
Name
Maximum
Voltage level1
1
+5V
+5
VDC
2
D-
+3.5
VDC
3
D+
+3.5
VDC
4
DGND
0
VDC
Metal
enclosure
Shield
(connected
to DGND)
0
VDC

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Table 2 Description of the NucleoCounter Printer Output connector
Pin no
Name
Maximum Voltage level2
13
2
Rx
±10 VDC
3
Tx
±10 VDC
Metal Enclosure
DGND
0 VDC
2
1
3
Metal
Enclosure
Figure 1 Printer Output connector seen from the cable entry side.
NucleoCounter is powered by an external 12VDC power supply. For safe use,
please follow the instructions for connecting the power supply.
The NucleoCounter shall only be used with one of the following external power
supplies:
Power supply Class I, Proton Electronic Industrial Co. Ltd., model SPN-260-
12, input rated 100-240 Vac, 50-60Hz, 1.6 A. Output rated 12 Vdc, 4.3 A.
Power Supply Class I, Powerbox Europe AB, model EBH 03 131, input rated
100-240 Vac, 47-63 Hz, 0.8A. Output rated 11-13 Vdc, 2.7 A.
Power supply Class II, Celetron USA Inc., model ZVC40LT12E, input rated
100-240 Vac, 50-60Hz, 1.2 A. Output rated 12 Vdc, 3.3 A.
The detachable power supply cord set and appliance inlet of the external power
supply are considered as the disconnecting device.
The mains supply cord and plug of the external power supply shall comply with
any national regulations.
2
In normal operation. Refers to DGND Metal Enclosure. Taken from the datasheet
for MAX202ECSE.
3
There is no signal connected to pin 1.

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The user shall be made aware of that, if the NucleoCounter and the external
power supply is used in a manner not specified by the manufacturer, the protection
provided by the NucleoCounter and the external power supply may be impaired.
Electromagnetic interference
NOTE: This equipment has been tested and found to comply with the limits for a
Class B digital device, pursuant to Part 15 of the FCC Rules. These limits are
designed to provide reasonable protection against harmful interference in a
residential installation. This equipment generates, uses and can radiate radio
frequency energy and, if not installed and used in accordance with the instructions,
may cause harmful interference to radio communications. However, there is no
guarantee that interference will not occur in a particular installation. If this
equipment does cause harmful interference to radio or television reception, which
can be determined by turning the equipment off and on, the user is encouraged to
try to correct the interference by one or more of the following measures:
Reorient or relocate the receiving antenna.
Increase the separation between the equipment and receiver.
Connect the equipment to an outlet on a circuit different from that to which
the receiver is connected.
Consult the dealer or an experienced radio/TV technician for help.
Caution! Changes or modifications not expressly approved by the party responsible
for compliance could void the user's authority to operate the equipment.
Cassettes, Reagents and Dispensers
With respect to usage and handling of cassettes, lysis buffers and dispensers,
please refer to appropriate package inserts for these items.
Caution! When using a bottle-top dispenser: To protect against accidental splashes
protective clothing, eye protection and gloves must be worn when using potentially
hazardous liquids.
General
Any biological specimen should be handled as if it is capable of transmitting
infectious disease and disposed of with proper precautions according with federal,
state and local regulations.
Avoid specimen contact with skin or mucous membranes.

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Never pipette by mouth.
Avoid cross contamination of the samples when preparing of the samples. This can
compromise the quality of the results.

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Table of contents
DECLARATION OF CONFORMITY ....................................................................... VI
WEEE DIRECTIVE INFORMATION –EUROPE ONLY .........................................VIII
INTRODUCTION AND INTENDED USE ................................................................. X
WARNINGS AND PRECAUTIONS.......................................................................... 1
1INSPECTION AND UNPACKING OF EQUIPMENT ........................................... 7
2INTRODUCING THE NUCLEOCOUNTER YC-100............................................ 9
2.1 THE NUCLEOCOUNTER YC-100 INSTRUMENT.................................................. 9
2.1.1 Fluorescence microscope...................................................................10
2.1.2 Built-in image analysis .......................................................................11
2.2 THE NUCLEOCASSETTE ..............................................................................11
2.2.1 Loading the cassette..........................................................................12
2.2.2 Fluorescent dye .................................................................................12
2.2.3 Sample volume ..................................................................................12
2.2.4 Cassette handling ..............................................................................13
2.3 LYSIS BUFFER............................................................................................13
3CONTROL BUTTONS, KEYPAD AND DISPLAY..............................................15
3.1 INTERACTIVE CONTROLS .............................................................................15
3.1.1 Control Buttons..................................................................................15
3.1.2 Keypad..............................................................................................15
3.2 INTERFACE DISPLAY ...................................................................................16
3.2.1 Status indicator..................................................................................16
3.2.2 Display screen...................................................................................16
4INSTALLATION AND START-UP....................................................................17
4.1 POWER ON,POWER OFF..............................................................................17
4.1.1 Starting up.........................................................................................17
4.1.2 Shutting down....................................................................................18
4.2 INSTALLATION OF NUCLEOVIEW ...................................................................18
5OPERATION OF THE NUCLEOCOUNTER YC-100 .........................................19
5.1 READY MODE.............................................................................................19
5.2 INSERTING AND REMOVING THE CASSETTE .....................................................19
5.2.1 Protection of the optical system .........................................................19
5.2.2 Inserting cassette ..............................................................................19
5.3 MEASURING -THE RUN BUTTON ...................................................................20
5.3.1 Actuator movements ..........................................................................20
5.3.2 Result presentation............................................................................20
5.3.3 External computer..............................................................................21
5.4 RESULT DISPLAY ........................................................................................21

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5.4.1 Result mode ......................................................................................21
5.4.2 Saving parameters.............................................................................22
5.4.3 Setting Date (F200) and Time (F201) .................................................22
5.4.4 Reset Counter (F30) ..........................................................................22
5.4.5 LCD contrast (F220) ..........................................................................22
5.4.6 Instrument Info (F100) .......................................................................23
5.5 INSTRUMENT SENSITIVITY (F11)...................................................................23
5.6 ZERO COUNT CHECK (F50) .........................................................................24
6TOTAL AND VIABILITY COUNT .....................................................................27
6.1 NON-VIABLE CELL CONCENTRATION .............................................................27
6.2 TOTAL CELL CONCENTRATION .....................................................................27
6.3 DETERMINATION OF VIABILITY......................................................................28
7MAINTENANCE OF NUCLEOCOUNTER YC-100 ............................................29
7.1 CLEANING .................................................................................................29
7.1.1 Cassette insertion area ......................................................................29
7.1.2 Optical elements................................................................................29
7.1.3 Removal of dust particles...................................................................30
7.1.4 Spill of liquid......................................................................................31
8TROUBLESHOOTING - ERROR MESSAGES..................................................33
8.1 NO VALID CASSETTE ...................................................................................33
8.2 ANALYSIS ABORTED....................................................................................33
8.3 ACTUATOR ERROR MESSAGES......................................................................34
8.4 SAMPLE COULD NOT BE ANALYZED................................................................34
8.5 SENSOR ERROR .........................................................................................34
8.6 POWER-ON FAILURE ...................................................................................35
9TECHNICAL SPECIFICATIONS......................................................................37
9.1 TECHNICAL SPECIFICATIONS FOR NUCLEOCOUNTER YC-100 ...........................37
9.2 THE NUCLEOCASSETTE™...........................................................................38
9.3 EMC/EMI STANDARDS................................................................................38
10 EQUIPMENT AND ACCESSORIES..............................................................38
10.1 EQUIPMENT AND ACCESSORIES LIST..........................................................38
10.2 POWER SUPPLY ......................................................................................40
10.3 MAINS POWER CORD ...............................................................................40
10.4 DRY COMPRESSED AIR.............................................................................41
10.5 EXTERNAL PRINTER (OPTIONAL)................................................................42
11 APPENDIX 1: WEEE DIRECTIVE INFORMATION IN MORE EU LANGUAGES
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1 Inspection and Unpacking of Equipment
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1 Inspection and Unpacking of Equipment
Upon receiving the order from ChemoMetec A/S, the box or boxes should be
carefully inspected for any damage that may have occurred during shipping. Any
damage must be reported to the carrier and to ChemoMetec A/S immediately.
Unpack the order, saving the packing materials for possible later use. Also be sure
to save the User’s Guide, for instruction and reference.
Verify the ChemoMetec packing list received refers to the correct and ordered
materials, and that nothing is missing.
If any part of the order was damaged during shipping or is missing, or fails to
operate, please contact ChemoMetec A/S.

1 Inspection and Unpacking of Equipment
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