Cole Parmer HG-600 Geno/Grinder 2010 User manual

1300-87055-1M, REV 6
HG-600 Geno/Grinder® 2010
Tissue and Cell Lyser for Biological Applications
Operation Manual
For 115V (04500-17) and 230V (04576-84)

HG-600 Geno/Grinder® 2010
1300-87055-1M, REV 6 Cole-Parmer® Page 2 of 36
Copyright 2023 by Cole-Parmer
All rights reserved. No part of the work may be reproduced or transmitted in any form or
by any means, electronic or mechanical; including photocopying and recording, except as
may be expressly permitted by the 1976 Copyright Act or in writing from the publisher.
Requests for permission should be addressed in writing to Cole-Parmer®, 65 Liberty Street,
Metuchen, NJ 08840.
SPEX SamplePrep is now part of Cole-Parmer®.
The Cole-Parmer® HG-600 Geno/Grinder was formerly known as SPEX 2010 Geno/Grinder.
Over the years, we’ve acquired many high-quality and reputable brands. After many years
of continual growth, we realize our brands are all as brilliant as each other. Rather than
have a portfolio of complementary brands, we felt consolidating them under one world-
class brand name enabled us to offer a single and significant brand experience. Through
one brand we can speak in one voice through our team of experts who provide support in
product selection, usage and troubleshooting to empower laboratories to run efficiently
throughout the world.
Same Great Quality!
One World-Class Brand Name!

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TABLE OF CONTENTS
SECTION
DESCRIPTION
PAGE
1.0 Introduction..................................................................................................................5
2.0 Specifications................................................................................................................6
3.0 Unpacking.....................................................................................................................7
4.0 Setting Up .....................................................................................................................8
4.1 Electrical Connection....................................................................................................8
4.2 Cabinet Set-Up..............................................................................................................8
4.3 Installing the Adjustable Clamp Assembly...................................................................8
5.0 Touchscreen Display...................................................................................................10
5.1 Logo Screen.................................................................................................................10
5.2 User List ......................................................................................................................11
5.3 Control Panel ..............................................................................................................13
5.4 Changing Settings .......................................................................................................15
5.5 Saved Protocols ..........................................................................................................16
6.0 Loading the Clamp......................................................................................................19
6.1 Loading the Adjustable Clamp....................................................................................20
7.0 Operation....................................................................................................................25
7.1 Preparing the Titer Plates...........................................................................................25
7.2 Operating the Lid........................................................................................................26
7.3 Running the Geno/Grinder® ......................................................................................26
7.4 Safety Recommendations ..........................................................................................27
8.0 Run History .................................................................................................................28
8.1 System Settings...........................................................................................................29
8.2 Administrative Lockout Feature.................................................................................30
8.3 Diagnostics..................................................................................................................31
9.0 Error Messages...........................................................................................................32
9.1 Lid Error.......................................................................................................................32
9.2 Rate Error....................................................................................................................32
10.0 Maintenance...............................................................................................................33
10.1 Changing the Fuses.....................................................................................................33

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11.0 Warranty.....................................................................................................................34
11.1 Product Specifications................................................................................................34
11.2 To Arrange a Return Shipment...................................................................................34
12.0 Instrument Disposal....................................................................................................35
13.0 Contact Us...................................................................................................................36

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1.0 INTRODUCTION
The HG-600 Geno/Grinder® was re-engineered with a new look and updated features. The
features include: programmable and operating commands through a series of touchscreen
displays, administrative lockout (passcode) which allows Lab Manager to restrict user access
to specific protocols, self-diagnostics, larger observation window, improved visibility of
sample loading area, and the new clamp orientation makes it easier to load/unload
samples.
The Geno/Grinder® is designed to effectively disrupt cellular materials by vertically
oscillating two or more deep-well titer plates, jars, tubes, or vials. This motion allows the
unit to be used to prepare sample tissue for extractions of nucleic acid, proteins, and other
constituents by agitating the tissue, steel balls or beads, and a lysis agent together in each
well of the titer plate, tube, or vial.
Operation is simple: the titer plates, jars, tubes, or vials are secured in a clamp, and the
cabinet lid closed. The controls are checked for the proper running time, stroke rate,
number of cycles, pause time and the RUN button pushed. When the run is complete, the
lid is lifted and the titer plates, jars, tubes, or vials are unclamped.
The isolation of nucleic acids from intact samples requires mechanically disrupting the
samples, followed by the extraction and subsequent purification of the nucleic acid.
Mechanical tissue disruption is often performed manually with a mortar and pestle, an
approach that is not practical for high-throughput screening since manual grinding of
tissues is slow, and re-use of mortars and pestles may lead to cross-contamination.
Alternatively, nucleic acids can be isolated in a multi-well titer plate, tubes, or vials using
balls or beads that mechanically disrupt the sample. Conventional isolation methodologies
can then be used to extract the nucleic acids from the homogenate.
Sample materials that can be prepared include seeds, stems, roots, leaves, and certain
animal tissue. Because the unique vertical shaking motion of the Geno/Grinder® is so
strong, many seeds and other forms of plant tissue can also be pulverized dry in titer plates,
jars, tubes, or vials with the help of one or two grinding balls per well, jar, tube or vial.
The Geno/Grinder® is also highly effective at improving extractions from food products,
such as for pesticide analysis according to the QuEChERS1method. A pre-cut fruit or
vegetable sample of 10-15 g is placed in a 50 ml conical centrifuge tube and the appropriate
salts and solvent are added. Two ceramic grinding cylinders are added; the tubes are
capped and shaken on the Geno/Grinder® for 1 min. Following clean-up, the samples are
analyzed by LC or GC-MS. Pesticide recovery in samples prepared using the Geno/Grinder®
has been found to be significantly improved over samples prepared manually.
1QuEChERS method developed by the USDA Eastern Regional Research Center. M. Anastassiades, S.J. Lehotay,
D. Stajnbaher, F.J. Schenck, J. AOAC International 86, p. 412-431 (2003).

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2.0 SPECIFICATIONS
Type of Grinder:
Homogenizer, Bead Beater
Display:
Touchscreen
Grinding
Mechanism:
Grinding balls of stainless steel, silica or zirconia beads.
Dimensions
28 in. (71.1 cm) high (40 in., 102 cm with lid open) x 14 in. (35.6 cm)
wide x 22.5 in. (57.2 cm) deep including handle.
Weight:
100 lbs. (45.5 kg)
Capacity:
Six standard 96 deep-well titer plates, sixteen 50 ml or twenty-four
15 ml centrifuge tubes, forty-eight 5 ml tubes or ninety-six 2 ml
microfuge tubes. Dynamic loads > 2 lbs. can create a rate error.
Clamp Speed:
Adjustable range: 500-1750 strokes/min.
Clamp Travel:
1.25 in. (3.2 cm)
Electrical
Specifications:
CE approved. 115V/230V, 60/50Hz
Fuse:
3AG 15-amp, 250 V slow-blow fuse for 115V/60Hz model.
3AG 10-amp, 250 V slow-blow fuse for 230V/50Hz model
Power Cord:
Do not plug mill into GFCI outlet (Ground Fault Circuit Interrupter)
115V/60Hz model: 3-prong grounded plug supplied.
230V/50Hz model: 2-prong European plug supplied.
Operator is responsible for supplying alternate line cord/plug.
Safety Features:
Lid locks while running. Interlock prevents mill from running if lid is
not closed. Manual lid release latch on the back of the unit.
Run Timer:
Maximum 20 minutes (1 cycle), Maximum 10 minutes (2 - 5 cycles).
Motor:
1/2 HP (3-Phase). Maximum speed 3280 rpm.
Maximum power 220 V.
Environment:
For indoor use only at a maximum altitude of 6600 ft. (2000 m).
Operate at ambient conditions between 40°F (5°C) and 104°F (40°C),
with maximum relative humidity 80% below 88°F (31°C) decreasing
linearly to 50% at 104°F (40°C). Main supply voltage fluctuations up
to 10% of nominal voltage (115 or 230 volts AC RMS). Pollution
degree 1: none or only dry, conductive pollution occurs.

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3.0 UNPACKING
Carefully inspect the exterior of the packing box. Any visible damage should immediately
be reported to the carrier. Remove all packed documents from the exterior of the box and
file in your records. Remove the top of the shipping box, the foam packaging material, and
the Geno/Grinder® accessories (power cord, titer plates, etc.). Lift the sleeve of the packing
box to remove it from the base of the box. Remove the top foam protector with the clamp
assembly inserted. Grasp the Geno/Grinder® on both sides, using proper lifting techniques,
and place it on a stable bench top (This is a 2-person job). Make sure that there is adequate
clearance on the sides to reach the AC inlet at the rear of the unit and on top when the lid is
fully open.
Follow a logical sequence of steps as you inspect the unit (see Figures 1 and 2).
1. Inspect the electrical input module for any visible damage.
2. Inspect the cabinet and lid for any visible damage.
3. Ensure that the controller is intact.
4. If the lid is locked, open by pressing and holding the Manual Release Latch on the
back of the unit (at the top left) while lifting the red Lid Handle at the same time.
5. Remove the cardboard/foam protector around the shaft inside the unit before
attaching the clamp assembly (refer to Section 4.3) or turning the unit on.
6. Check the clamp assembly (packed separately). Ensure that it is undamaged.
7. Inspect the accessories and compare with the packing list.
If everything appears to be in proper order,
store the packaging materials in case there is a
need to return the unit for service or repair.
Observation
Window
Figure 1
Figure 2
2012 Clamp
Power Button
Lid Handle
Controller
AC Inlet
(Back of unit)
USB
Manual Lid
Release Latch
(Back of unit)

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4.0 SETTING UP
The HG-600 Geno/Grinder® weighs 100 pounds (45.5 Kg). The lid opens from the front with
the handle in the center. The AC Input module, fuse tray, and power cord receptacle are
located on the rear of the Geno/Grinder® cabinet, toward its bottom. The power (On/Off)
button is on the front right, as shown in Figure 2. The Manual Lid Release Latch is located
on the rear of the unit at the top left. There are gas springs on both sides of the cabinet to
control the movement of the lid. The touchscreen controller is affixed to the front side of
the unit. Under the front edge of the controller is a USB port to export run history, or
upgrade system software (Figure 1).
4.1 Electrical Connection
The detachable power cord should be plugged firmly into its inlet, then into an electrical
outlet. Do not plug mill into a GFCI (Ground Fault Circuit Interrupter) outlet.Make sure
that the electrical outlet is easily accessible in case it becomes necessary to unplug the unit.
For 115V/60HZ use, a 3-prong outlet fused at 20 amps is recommended. The 230V/50HZ
Geno/Grinder® power cord has a standard European 2-prong plug, but modification by the
user may be necessary to meet local electrical codes.
4.2 Cabinet Set-Up
To open the cabinet, grasp the red handle and raise the lid to its full upright position. The
gas spring will hold the lid in its open position. To close, grasp the red handle and lower the
lid until it is fully closed.
A safety interlock will engage the Geno/Grinder® only when the lid is closed. If the RUN
button is touched while the lid is open, the Geno/Grinder® will not start. The lid stays
locked when the unit is running which prevents the user from opening it during operation.
If the locking mechanism fails and the lid is opened, the motor will immediately stop, and
movement of the clamp assembly will cease.
4.3 Installing the Clamp Assembly
The 2012 adjustable clamp can accommodate a variety of titer plates (up to six) and vial
sizes. This clamp assembly is also used with 15 ml or 50 ml centrifuge tubes. The
Geno/Grinder® clamp can also be adapted to hold anything with the same general
dimensions (5 in. long x 3 in. wide x 2 to 4 in. high). This includes titer plates with fewer
and/or larger wells, racks that hold multiple individual vials, and other possible
configurations. Spacers are not required for the 2012 adjustable clamp.
To prevent damage during shipping, the clamp assembly is not installed at the factory.
Before operation, this must be installed by the user.

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4.3.1 Installing the Adjustable Clamp Assembly
To install the clamp, first locate the 3/16” Allen wrench and the two bolts and lock washers
supplied with the Geno/Grinder®. Open the Geno/Grinder® lid and place the clamp base on
top of the shaft. Using the lock washers and bolts, secure the clamp base to the shaft with
the Allen wrench (Figure 3).
IMPORTANT: THE BOLTS AND LOCK WASHERS SUPPLIED WITH THIS CLAMP MUST BE
USED!!
NOTE: PLEASE DO NOT OPERATE THE GENO/GRINDER® UNTIL YOU HAVE READ THESE
INSTRUCTIONS AND ARE FAMILIAR WITH ITS CONTROLS AND CLAMP MECHANISM.
THE GENO/GRINDER® IS INTENDED FOR USE ONLY BY QUALIFIED AND TRAINED
PERSONNEL.
Figure 3 –Installing the Adjustable Clamp
Upright must face the
rear of the
Geno/Grinder®
Secure clamp base to shaft
using bolts and lock washers
supplied with this clamp
Shaft attached to the
Geno/Grinder®

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5.0 TOUCHSCREEN DISPLAY
The HG-600 Geno/Grinder® is programmed and operated through a series of touchscreen
displays. Transition between screens, and all programming and operating commands, are
done by touching the screen with a fingertip or stylus. Do not use a sharp point as this can
damage or deface the screen.
5.1 Logo Screen
When the power is switched ON at the front of the mill, “Please wait” appears during start-
up as the software loads. The screen will switch to the Home Screen, as shown in Figure 4.
From the Home Screen the User Name, Control Panel, Saved Protocols, Run History, and
Settings can be accessed by touching the buttons displayed.
The Home Screen Icon can be found on the Control Panel positioned at the bottom right of
the screen. Touching the Home Icon allows the user to return to the Home Screen.
Figure 4 –Home Screen

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5.2 User List
To access the USER LIST screen touch the Username button on the Home Screen. An
infinite number of users can be stored. Only 10 user slots at one-time are visible on the
User List screen (Figure 5). To access or make visible additional users, or vacant spaces
swipe up or down the touchscreen. This brings up vacant slots on the User List screen.
Touching the Back Arrow button (top left) allows the user to return to the previous screen.
5.2.1 Recalling a User
In the USER LIST Screen, touch the username to highlight the name. Then touch the Back
Arrow button at the top left of the screen to send the username to the CONTROL PANEL
Screen. The username will appear below the Date and Time at the bottom right of the
screen, as shown in Figure 6.
Figure 5 –User List Screen
Figure 6 –Control Panel

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5.2.2 Add a User
To add a new user, touch the add button on the User List Screen (Figure 5) which will bring
up the EDIT/ADD USER Screen. Touch inside the Box, as shown in Figure 7. Touching the
Back Arrow button (top left) allows the user to return to the previous screen.
This will bring up the KEYBOARD screen, a simplified version of the standard keyboard for a
computer. In addition to number and letter keys, this keyboard has standard symbol keys
(#, %) and four function keys (SPACE, BACK, DONE, and CLEAR).
The CLEAR key deletes whatever has been entered in the Box. As the letters/numbers are
touched, they appear above the keyboard in the Box with a centered cursor. To access the
number and symbol keys touch the 12# button located bottom left on the KEYBOARD
screen. To switch back to letter keys touch the ABC button (same button) bottom left on
the KEYBOARD screen.
The Up/Down yellow arrow key allows the user to shift back and forth from upper case to
lower case (the default is upper case). Touch Down arrow to switch to lower case. Or
touch Up arrow to revert to upper case.
Touching the SPACE key advances the cursor one space. Touching the BACK key deletes one
space. Touching the DONE key enters the label shown in the box.
Touch the SAVE USER button to save the label shown in the Box. The label will appear as
the username in the USER LIST Screen (Figure 5). The CANCEL key does not change
anything on the screen, except returns the display to the USER LIST Screen.
Figure 7 –Edit/Add User Screen
Touch inside
the box
Touch inside
the Box

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5.2.3 Edit a User
To edit a saved user, touch the username to highlight the name on the User List screen
(Figure 5). Then touch the EDIT User button. From the Edit/Add User screen change the
name by touching inside the box. This will bring up the KEYBOARD screen, follow the
instructions listed in the Add a User SECTION 5.2.2.Note, changes cannot be made to the
Guest field.
5.2.4 Delete a User
To delete a saved user, touch the username to highlight the name on the User List screen
(Figure 5). Then touch the Delete button. A pop-up window opens confirming to delete
this user. Touch YES to clear or touch NO to retain the username.
5.3 Control Panel
The Control Panel displays the programmed run parameters, Date & Time and User name at
the bottom right of the screen, Protocol name at the bottom center of the screen, as shown
in Figure 8. Changes to the run settings, are made from this screen.
To recall stored run protocols, touch the button located at the bottom right of the screen.
(See section 5.5 for more information on Saved Protocols)
--To return to the Home Screen touch the Home Icon.
Figure 8 –Control Panel

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5.3 Control Panel (Cont’d)
The following sequence of events will occur from the current settings in Figure 8.
1. Cycle 1 - the unit runs for 2 minutes at a rate of 1000 strokes per minute.
2. Pause - the unit is inactive for 30 seconds allowing the samples to undergo lysis.
3. Cycle 2 - the unit runs for another 2 minutes at a rate of 1000 strokes per minute.
In the CONTROL PANEL, The STATUS BAR (top of screen) counts down the time remaining
for a run (Figure 9).
To change the settings of a selected field, press the plus and minus buttons on the
CONTROL PANEL Screen. The minus buttons decrease the number displayed and the plus
buttons increase the number displayed.
5.3.1 Starting a Programmed Run
To run the program displayed on the CONTROL PANEL Screen, touch the run button. The
Geno/Grinder® can be started, stopped, or paused in the middle of a grinding program from
the button selections to the right of the screen.
Figure 9 –Run Screen (time remaining)

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5.3 Control Panel (Cont’d)
During the RUN TIME stage the TIME REMAINING line counts down the time for the
programmed run in 1-second increments. To pause the program, touch the pause button.
To continue the program, touch the RESUME button. To abort the program, touch the
STOP button.
To start a new program, touch the RUN button.
The RUNTIME line will display the time remaining in that cycle of the grinding stage. If
there are 2 total grinding periods, the CYCLES line will countdown to “0”. The maximum
number of cycles is 5.
5.3.2 Stopping or Pausing a Run
To stop a program, touch the stop button on the RUN SCREEN (Figure 9), the button will
dim or turn a muted red and the program will end. If the RUN button is touched after the
program has stopped then the entire program will start over.
To pause during a grinding program, touch the PAUSE button. Note, the TIME REMANING
line will turn yellow which indicates runtime is inactive. The run will hold the settings at
that moment (elapsed time, cycle, etc.). At this point the door to the sample area can be
opened. To resume the program, touch the RESUME button. This will restart the program
at the point at which it was paused. Alternatively, pressing STOP will end the program.
5.4 Changing Settings
To change parameters (run time, pause time, cycles, and rate), touch the plus and minus
buttons on the CONTROL PANEL Screen. Touching the plus button increases the parameter,
while touching the minus button decreases the parameter. Tap or hold the plus or minus
buttons until the desired number is reached. The changes will appear in the blue area
between the plus and minus, as shown in Figure 10. The maximum run time is 20 minutes
for 1 cycle. If running 2 - 5 cycles the maximum run time per cycles is 10 minutes. The
minimum Rest is 15 seconds per cycle for 2 - 5 cycles. To run a program with the new
settings, touch the run button.
NOTE: If the run parameters are changed to a Protocol and not saved then (MOD) will
appear to the right of the Protocol name at the bottom center of the screen.

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Figure 10 –Changing Settings
5.4 Changing Settings (Cont’d)
5.5 Saved Protocols
The SAVED PROTOCOLS Screen is shown in Figure 11. Up to 500 Protocols can be saved for
simple and fast recall, increasing productivity and reducing operator error. Only 20
protocols are visible at one-time on the Saved Protocols Screen. To view additional
protocols, or vacant protocol spaces, swipe up or down on the touchscreen. This brings up
vacant protocol slots on the SAVED PROTOCOLS Screen.
A saved program retains the settings for number of cycles, run time, pause time between
cycles, and rate. Be sure to adjust the rate to the desired setting after recalling a stored
program.
Store and
Recall
Protocols
(+) plus
Increases the
Parameter
Home Screen
Icon
(-) minus
Decreases the
Parameter

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5.5 Saved Protocols (Cont’d)
The Default protocol recalls the last programed run setting that was not saved. The Default
protocol can be changed without saving the new settings, as described in section 5.4. At
the top left on the Saved Protocols Screen is the Back Arrow button. Touching the Back
Arrow button returns the user to the previous screen.
5.5.1 Recalling a Protocol
To recall a stored program, touch the button on the Control Panel Screen (Figure 10). In the
SAVED PROTOCOLS Screen, select the protocol to highlight the box. Then touch SELECT to
send protocol to the CONTROL PANEL Screen (Figure 11).
5.5.2 Storing a New Protocol
To store program, touch the button on the Control Panel Screen (Figure 10). In the SAVED
PROTOCOLS Screen, touch the add button, as shown in Figure 11.
This will bring up the KEYBOARD screen, a simplified version of the standard keyboard for a
computer.
Touch inside
the Label Box
Figure 11 –Saved Protocols Screen

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5.5 Saved Protocols (Cont’d)
The CLEAR key deletes whatever has been entered in the Label Box.
Touching the DONE key enters the label shown in the box.
Touch the SAVE button to save the label shown in the Box. The label will appear as the
name of the program in the SAVED PROTOCOLS Screen (Figure 11).
The CANCEL key does not change anything on the screen, except returns the display to the
SAVED PROTOCOLS Screen.
To run the newly saved protocol, touch the protocol to highlight the box. Then touch
SELECT to send the protocol to the CONTROL PANEL Screen. Review the parameters and
touch the RUN button to initiate the protocol.
5.5.3 Edit a Protocol
To edit a saved protocol, touch the protocol name to highlight the box on the Saved
Protocol screen (Figure 11). Then touch the EDIT button. From the Edit Protocol Screen
change the name by touching inside the box. This will bring up the KEYBOARD screen,
follow the instructions listed in the Storing a Protocol SECTION 5.5.2.
Figure 12 –Edit Protocols Screen
Touch inside the
Label Box to
change name

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5.5.4 Delete a Protocol
To delete a saved protocol, touch the protocol name to highlight the name on the Saved
Protocols Screen (Figure 11). Then touch the Delete button. A pop-up window opens
confirming to delete this protocol. Touch YES to clear or touch NO to keep the saved
protocol, as shown in Figure 13.
5.5.5 Modify a Recalled Protocol
Once a saved protocol is recalled to the CONTROL PANEL Screen, the User can modify the
parameters by using the plus (+) and minus (-) buttons. If the protocol is modified, (MOD)
will appear next to the protocol name, e.g. WHEAT(MOD).
6.0 LOADING THE CLAMP
Lock Sample Holder!
This warning symbol is visible in front of the clamp
assembly. It is essential that samples are securely
locked into the sample holder (clamp assembly) before
operation.
Figure 13 –Protocols Deletion Screen

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6.1 Loading the Adjustable Clamp
The clamp is the most critical component of the HG-600 Geno/Grinder®, as it must be
carefully adjusted to hold the titer plates (or vials, centrifuge tubes, etc.) firmly in place.
The up-and-down motion of the clamp is extremely vigorous. Sample holders (titer plates,
vials, centrifuge tubes, etc.) must be held securely in the clamp during operation to prevent
damage and leakage.
6.1.1 Vials in Holders or Cryo-Blocks
1. Place the sample vials (in the appropriate holder or cryo-block) in the clamp base
(Figure 14). The sample load must be evenly distributed at all sides of the clamp to
avoid excess wear to the Geno/Grinder®. Also, the clamp lid must have a flat
surface upon which to rest. If running less than a full holder of vials, be sure to place
vials at the 4 corners to support the clamp lid.
2. Slide the lid on top of the vials. Screw down the locking knob onto the threaded
screw and the guide onto the upright at the back of the clamp (Figure 15).
3. Turn the knob clockwise until finger tight to secure the vials in the clamp. It is not
necessary to over tighten the clamp lid. Check to see whether the vials can be
moved from side to side in the clamp. If not, the clamp is tight and samples are
secure. If the vials can be moved, tighten the handle gradually until the vials are
secure.
4. Close the Geno/Grinder® lid and run the unit.