Cole Parmer HG-600 Geno/Grinder 2010 User manual

1300-87055-1M, REV 6
HG-600 Geno/Grinder® 2010
Tissue and Cell Lyser for Biological Applications
Operation Manual
For 115V (04500-17) and 230V (04576-84)

HG-600 Geno/Grinder® 2010
1300-87055-1M, REV 6 Cole-Parmer® Page 2 of 36
Copyright 2023 by Cole-Parmer
All rights reserved. No part of the work may be reproduced or transmitted in any form or
by any means, electronic or mechanical; including photocopying and recording, except as
may be expressly permitted by the 1976 Copyright Act or in writing from the publisher.
Requests for permission should be addressed in writing to Cole-Parmer®, 65 Liberty Street,
Metuchen, NJ 08840.
SPEX SamplePrep is now part of Cole-Parmer®.
The Cole-Parmer® HG-600 Geno/Grinder was formerly known as SPEX 2010 Geno/Grinder.
Over the years, we’ve acquired many high-quality and reputable brands. After many years
of continual growth, we realize our brands are all as brilliant as each other. Rather than
have a portfolio of complementary brands, we felt consolidating them under one world-
class brand name enabled us to offer a single and significant brand experience. Through
one brand we can speak in one voice through our team of experts who provide support in
product selection, usage and troubleshooting to empower laboratories to run efficiently
throughout the world.
Same Great Quality!
One World-Class Brand Name!

HG-600 Geno/Grinder® 2010
1300-87055-1M, REV 6 Cole-Parmer® Page 3 of 36
TABLE OF CONTENTS
SECTION
DESCRIPTION
PAGE
1.0 Introduction..................................................................................................................5
2.0 Specifications................................................................................................................6
3.0 Unpacking.....................................................................................................................7
4.0 Setting Up .....................................................................................................................8
4.1 Electrical Connection....................................................................................................8
4.2 Cabinet Set-Up..............................................................................................................8
4.3 Installing the Adjustable Clamp Assembly...................................................................8
5.0 Touchscreen Display...................................................................................................10
5.1 Logo Screen.................................................................................................................10
5.2 User List ......................................................................................................................11
5.3 Control Panel ..............................................................................................................13
5.4 Changing Settings .......................................................................................................15
5.5 Saved Protocols ..........................................................................................................16
6.0 Loading the Clamp......................................................................................................19
6.1 Loading the Adjustable Clamp....................................................................................20
7.0 Operation....................................................................................................................25
7.1 Preparing the Titer Plates...........................................................................................25
7.2 Operating the Lid........................................................................................................26
7.3 Running the Geno/Grinder® ......................................................................................26
7.4 Safety Recommendations ..........................................................................................27
8.0 Run History .................................................................................................................28
8.1 System Settings...........................................................................................................29
8.2 Administrative Lockout Feature.................................................................................30
8.3 Diagnostics..................................................................................................................31
9.0 Error Messages...........................................................................................................32
9.1 Lid Error.......................................................................................................................32
9.2 Rate Error....................................................................................................................32
10.0 Maintenance...............................................................................................................33
10.1 Changing the Fuses.....................................................................................................33

HG-600 Geno/Grinder® 2010
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11.0 Warranty.....................................................................................................................34
11.1 Product Specifications................................................................................................34
11.2 To Arrange a Return Shipment...................................................................................34
12.0 Instrument Disposal....................................................................................................35
13.0 Contact Us...................................................................................................................36

HG-600 Geno/Grinder® 2010
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1.0 INTRODUCTION
The HG-600 Geno/Grinder® was re-engineered with a new look and updated features. The
features include: programmable and operating commands through a series of touchscreen
displays, administrative lockout (passcode) which allows Lab Manager to restrict user access
to specific protocols, self-diagnostics, larger observation window, improved visibility of
sample loading area, and the new clamp orientation makes it easier to load/unload
samples.
The Geno/Grinder® is designed to effectively disrupt cellular materials by vertically
oscillating two or more deep-well titer plates, jars, tubes, or vials. This motion allows the
unit to be used to prepare sample tissue for extractions of nucleic acid, proteins, and other
constituents by agitating the tissue, steel balls or beads, and a lysis agent together in each
well of the titer plate, tube, or vial.
Operation is simple: the titer plates, jars, tubes, or vials are secured in a clamp, and the
cabinet lid closed. The controls are checked for the proper running time, stroke rate,
number of cycles, pause time and the RUN button pushed. When the run is complete, the
lid is lifted and the titer plates, jars, tubes, or vials are unclamped.
The isolation of nucleic acids from intact samples requires mechanically disrupting the
samples, followed by the extraction and subsequent purification of the nucleic acid.
Mechanical tissue disruption is often performed manually with a mortar and pestle, an
approach that is not practical for high-throughput screening since manual grinding of
tissues is slow, and re-use of mortars and pestles may lead to cross-contamination.
Alternatively, nucleic acids can be isolated in a multi-well titer plate, tubes, or vials using
balls or beads that mechanically disrupt the sample. Conventional isolation methodologies
can then be used to extract the nucleic acids from the homogenate.
Sample materials that can be prepared include seeds, stems, roots, leaves, and certain
animal tissue. Because the unique vertical shaking motion of the Geno/Grinder® is so
strong, many seeds and other forms of plant tissue can also be pulverized dry in titer plates,
jars, tubes, or vials with the help of one or two grinding balls per well, jar, tube or vial.
The Geno/Grinder® is also highly effective at improving extractions from food products,
such as for pesticide analysis according to the QuEChERS1method. A pre-cut fruit or
vegetable sample of 10-15 g is placed in a 50 ml conical centrifuge tube and the appropriate
salts and solvent are added. Two ceramic grinding cylinders are added; the tubes are
capped and shaken on the Geno/Grinder® for 1 min. Following clean-up, the samples are
analyzed by LC or GC-MS. Pesticide recovery in samples prepared using the Geno/Grinder®
has been found to be significantly improved over samples prepared manually.
1QuEChERS method developed by the USDA Eastern Regional Research Center. M. Anastassiades, S.J. Lehotay,
D. Stajnbaher, F.J. Schenck, J. AOAC International 86, p. 412-431 (2003).

HG-600 Geno/Grinder® 2010
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2.0 SPECIFICATIONS
Type of Grinder:
Homogenizer, Bead Beater
Display:
Touchscreen
Grinding
Mechanism:
Grinding balls of stainless steel, silica or zirconia beads.
Dimensions
28 in. (71.1 cm) high (40 in., 102 cm with lid open) x 14 in. (35.6 cm)
wide x 22.5 in. (57.2 cm) deep including handle.
Weight:
100 lbs. (45.5 kg)
Capacity:
Six standard 96 deep-well titer plates, sixteen 50 ml or twenty-four
15 ml centrifuge tubes, forty-eight 5 ml tubes or ninety-six 2 ml
microfuge tubes. Dynamic loads > 2 lbs. can create a rate error.
Clamp Speed:
Adjustable range: 500-1750 strokes/min.
Clamp Travel:
1.25 in. (3.2 cm)
Electrical
Specifications:
CE approved. 115V/230V, 60/50Hz
Fuse:
3AG 15-amp, 250 V slow-blow fuse for 115V/60Hz model.
3AG 10-amp, 250 V slow-blow fuse for 230V/50Hz model
Power Cord:
Do not plug mill into GFCI outlet (Ground Fault Circuit Interrupter)
115V/60Hz model: 3-prong grounded plug supplied.
230V/50Hz model: 2-prong European plug supplied.
Operator is responsible for supplying alternate line cord/plug.
Safety Features:
Lid locks while running. Interlock prevents mill from running if lid is
not closed. Manual lid release latch on the back of the unit.
Run Timer:
Maximum 20 minutes (1 cycle), Maximum 10 minutes (2 - 5 cycles).
Motor:
1/2 HP (3-Phase). Maximum speed 3280 rpm.
Maximum power 220 V.
Environment:
For indoor use only at a maximum altitude of 6600 ft. (2000 m).
Operate at ambient conditions between 40°F (5°C) and 104°F (40°C),
with maximum relative humidity 80% below 88°F (31°C) decreasing
linearly to 50% at 104°F (40°C). Main supply voltage fluctuations up
to 10% of nominal voltage (115 or 230 volts AC RMS). Pollution
degree 1: none or only dry, conductive pollution occurs.

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3.0 UNPACKING
Carefully inspect the exterior of the packing box. Any visible damage should immediately
be reported to the carrier. Remove all packed documents from the exterior of the box and
file in your records. Remove the top of the shipping box, the foam packaging material, and
the Geno/Grinder® accessories (power cord, titer plates, etc.). Lift the sleeve of the packing
box to remove it from the base of the box. Remove the top foam protector with the clamp
assembly inserted. Grasp the Geno/Grinder® on both sides, using proper lifting techniques,
and place it on a stable bench top (This is a 2-person job). Make sure that there is adequate
clearance on the sides to reach the AC inlet at the rear of the unit and on top when the lid is
fully open.
Follow a logical sequence of steps as you inspect the unit (see Figures 1 and 2).
1. Inspect the electrical input module for any visible damage.
2. Inspect the cabinet and lid for any visible damage.
3. Ensure that the controller is intact.
4. If the lid is locked, open by pressing and holding the Manual Release Latch on the
back of the unit (at the top left) while lifting the red Lid Handle at the same time.
5. Remove the cardboard/foam protector around the shaft inside the unit before
attaching the clamp assembly (refer to Section 4.3) or turning the unit on.
6. Check the clamp assembly (packed separately). Ensure that it is undamaged.
7. Inspect the accessories and compare with the packing list.
If everything appears to be in proper order,
store the packaging materials in case there is a
need to return the unit for service or repair.
Observation
Window
Figure 1
Figure 2
2012 Clamp
Power Button
Lid Handle
Controller
AC Inlet
(Back of unit)
USB
Manual Lid
Release Latch
(Back of unit)

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4.0 SETTING UP
The HG-600 Geno/Grinder® weighs 100 pounds (45.5 Kg). The lid opens from the front with
the handle in the center. The AC Input module, fuse tray, and power cord receptacle are
located on the rear of the Geno/Grinder® cabinet, toward its bottom. The power (On/Off)
button is on the front right, as shown in Figure 2. The Manual Lid Release Latch is located
on the rear of the unit at the top left. There are gas springs on both sides of the cabinet to
control the movement of the lid. The touchscreen controller is affixed to the front side of
the unit. Under the front edge of the controller is a USB port to export run history, or
upgrade system software (Figure 1).
4.1 Electrical Connection
The detachable power cord should be plugged firmly into its inlet, then into an electrical
outlet. Do not plug mill into a GFCI (Ground Fault Circuit Interrupter) outlet.Make sure
that the electrical outlet is easily accessible in case it becomes necessary to unplug the unit.
For 115V/60HZ use, a 3-prong outlet fused at 20 amps is recommended. The 230V/50HZ
Geno/Grinder® power cord has a standard European 2-prong plug, but modification by the
user may be necessary to meet local electrical codes.
4.2 Cabinet Set-Up
To open the cabinet, grasp the red handle and raise the lid to its full upright position. The
gas spring will hold the lid in its open position. To close, grasp the red handle and lower the
lid until it is fully closed.
A safety interlock will engage the Geno/Grinder® only when the lid is closed. If the RUN
button is touched while the lid is open, the Geno/Grinder® will not start. The lid stays
locked when the unit is running which prevents the user from opening it during operation.
If the locking mechanism fails and the lid is opened, the motor will immediately stop, and
movement of the clamp assembly will cease.
4.3 Installing the Clamp Assembly
The 2012 adjustable clamp can accommodate a variety of titer plates (up to six) and vial
sizes. This clamp assembly is also used with 15 ml or 50 ml centrifuge tubes. The
Geno/Grinder® clamp can also be adapted to hold anything with the same general
dimensions (5 in. long x 3 in. wide x 2 to 4 in. high). This includes titer plates with fewer
and/or larger wells, racks that hold multiple individual vials, and other possible
configurations. Spacers are not required for the 2012 adjustable clamp.
To prevent damage during shipping, the clamp assembly is not installed at the factory.
Before operation, this must be installed by the user.

HG-600 Geno/Grinder® 2010
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4.3.1 Installing the Adjustable Clamp Assembly
To install the clamp, first locate the 3/16” Allen wrench and the two bolts and lock washers
supplied with the Geno/Grinder®. Open the Geno/Grinder® lid and place the clamp base on
top of the shaft. Using the lock washers and bolts, secure the clamp base to the shaft with
the Allen wrench (Figure 3).
IMPORTANT: THE BOLTS AND LOCK WASHERS SUPPLIED WITH THIS CLAMP MUST BE
USED!!
NOTE: PLEASE DO NOT OPERATE THE GENO/GRINDER® UNTIL YOU HAVE READ THESE
INSTRUCTIONS AND ARE FAMILIAR WITH ITS CONTROLS AND CLAMP MECHANISM.
THE GENO/GRINDER® IS INTENDED FOR USE ONLY BY QUALIFIED AND TRAINED
PERSONNEL.
Figure 3 –Installing the Adjustable Clamp
Upright must face the
rear of the
Geno/Grinder®
Secure clamp base to shaft
using bolts and lock washers
supplied with this clamp
Shaft attached to the
Geno/Grinder®

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5.0 TOUCHSCREEN DISPLAY
The HG-600 Geno/Grinder® is programmed and operated through a series of touchscreen
displays. Transition between screens, and all programming and operating commands, are
done by touching the screen with a fingertip or stylus. Do not use a sharp point as this can
damage or deface the screen.
5.1 Logo Screen
When the power is switched ON at the front of the mill, “Please wait” appears during start-
up as the software loads. The screen will switch to the Home Screen, as shown in Figure 4.
From the Home Screen the User Name, Control Panel, Saved Protocols, Run History, and
Settings can be accessed by touching the buttons displayed.
The Home Screen Icon can be found on the Control Panel positioned at the bottom right of
the screen. Touching the Home Icon allows the user to return to the Home Screen.
Figure 4 –Home Screen