Cole Parmer 81150-48 User manual

Operating Manual
Handheld Digital Refractometers
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2
LCD DISPLAY
KEYS (BUTTONS)
1. Turn on meter; or
2. Turn off meter after pressing for 3 seconds.
1. Zero calibration; or
2. Press for 2 seconds to toggle between °C and °F.
1. Press the button to read after drop samples; or
2. Press for 2 seconds to toggle between measured
items.
+Reset to factory setting.
INTRODUCTION
Thank you for choosing our refractometer. This is an easy-to-use
device, requiring little to no training. Please read the manual before
using to ensure optimal measurement.
The refractometer is designed for fast and accurate determination of
fluid concentrations. The instrument will automatically compensate
for temperature fluctuation and can be used for years of trouble-free
service when properly and regularly maintained. Instrument has
an IP65 waterproof rating.
The refractometer represents reading relative to Brix scale. The Brix
scale originated in the food industry and is primarily a unit of measure
corresponding to percent of sugar in a sugar and water solution. The
actual Brix value represents the number of grams of cane sugar in a
100-g cane-sugar solution (percent sugar wt./wt.). This direct reading
relationship holds true only for sucrose solutions. When measuring
non-sugar solutions, the Brix scale should be thought of as an arbitrary
scale. To make these arbitrary units of measure meaningful, they must
somehow be correlated to the concentration on the solution you are
testing. This is accomplished by creating a chart of solution concentra-
tion relative to the Brix scale. A separate must be made to each type of
solution being tested.
Unpacking
Check individual parts against the list of items below. If anything
is missing or damaged, please contact your instrument supplier
immediately.
1. Refractometer
2. Soft and clean cloth
3. Hard carrying case
4. Operation manual
5. Two AAA batteries
SPECIFICATIONS
Temperature measurement range: 32 to 104°F (0 to 40°C)
Measurement range: Please see table above.
Precision of measurement temperature: ±1°F (0.5°C)
Automatic temperature compensation: 41 to 104°F (5 to 40°C)
Minimum sample volume: 0.3 mL
Measuring response time: ≤3 seconds
Power supply: two AAA batteries
Battery life: ≥5000 readings
Dimensions (L x W x H): 5.7" x 2.6" x 1.5"(145 × 67 × 38 mm)
Weight: 6.5 oz (185 g)
AUTO TEMPERATURE COMPENSATION (ATC)
Refractive index (RI) is very temperature-dependent. It is well known
that substantially all materials expand when heated (i.e. become less
dense) and contract when cooled (i.e. become more dense). The speed
of light in a liquid increases as temperature increases, and refractive
index, therefore, decreases. Although this thermal effect is minor for
solids, the change in density for a liquid is substantial. ATC ensures
that concentration readings of aqueous (water-based) solutions will
be accurate with respect to sample's temperature.
The refractometer is temperature compensated for aqueous (water-
based) sucrose solutions and can automatically compensate for
temperature differences within the range of 41 to 104°F (5 to 40°C). The
temperature of the sample, however, has little bearing on the accuracy
of the reading. In most cases, the sample almost immediately assumes
the temperature of the refractometer, the ambient temperature, and the
fluid should be in equilibrium within the instrument's temperature
range.
Range Resolution Accuracy ATC Catalog
number
0 to 45% Brix, 1.3330 to 1.4098 RI 0.1, 0.0001 ±0.2, ±0.0003 Yes 81150-48
0 to 85% Brix, 1.3330 to 1.5100 RI 0.1, 0.0001 ±0.5, ±0.0003 Yes 81150-55
0 to 95% Brix, 1.3330 to 1.5400 RI 0.1, 0.0001 ±0.5, ±0.0003 Yes 81150-56
0 to 28% NaCl, 1.3330 to 1.4100 RI 0.1, 0.0001 ±0.2, ±0.0003 Yes 81150-49
0 to 45% Brix, 0 to 28% NaCl,
1.3330 to 1.4100 RI 0.1, 0.1, 0.0001 ±0.2, ±0.2, ±0.0003 Yes 81150-57
0 to 60% v/v, w/w ethyl alcohol 0.1 ±0.3 Yes 81150-52
0 to 60% v/v, w/w, isopropyl
alcohol; –58 to 32°F, –50 to 0°C 0.1 ±0.5 Yes 81150-53
0 to 75% ethylene glycol,
–58 to 32°F, –50 to 0°C 0.1 ±0.3, ±1°F, ±0.5°C Yes 81150-54
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3
CALIBRATION: ZERO SET
The refractometer must be set to zero before initial use and periodically
thereafter. It is recommended that calibration be performed at least
once a day as well as prior to performing tests requiring the highest
precision, or when moving between environments with extreme
changes in ambient temperature. A clean container of water is all that
is needed to automatically calibrate the instrument. Although tap water
may be used, distilled or deionized water is recommended. The water
temperature for calibration should ideally near 68°F (20°C).
1. Inspect the measuring surface to make sure
it is clean and dry.
2. Place a few drops of calibration liquids on the
prism window, making sure to eliminate any
bubbles.
3. Press to start calibration.
4. The unit shows the process of zero setting
(Fig 2.1).
5. If calibration is successful, it will show “Pass”
(Fig 2.2).
6. If calibration is not successful, it will show “Fail”.
7. After calibration process is finished, the LCD will
return to temperature mode. The calibration result
will be saved and will be the new zero point after
the device is powered off and on again.
Note: After calibration, please remember to clean the liquid from
the prism surface and keep the prism surface dry and clean to
avoid erosion.
MEASUREMENT
1. Make sure the prism surface is clean and dry.
2. Place a few drops of the sample on the prism.
3. Press key, “---” will be shown on the screen.
– Test result(s) will be shown on the screen
(Fig 3.1).
– The result(s) will be stored in the device
for up to 60 seconds.
– Press the “Power” button to recheck the previous measurements.
4. After testing, please remember to clean and dry the surface when
you are done.
Scale Selection
1. Hold for 2 seconds, the scale will change to
the next measurement type (Fig 4.1).
2. Repeat Step 1 until desired scale is shown
on the screen.
3. The device saves the last selected scale.
Temperature Selection
1. Hold for 2 seconds, the temperature unit will change between
°F and °C.
2. The device saves the last selected temperature unit.
Resetting
1. Press + , the option of returning to factory
setting of zero will be shown on the screen
(Fig 5.1).
2. Press button to confirm resetting, or
press to cancel.
Fig 2.1
Fig 2.2
Fig 3.1
STATUS
1. Press to turn on the device.
2. Press to turn off the device; the LCD will
show the process (Fig 6.1).
3. To conserve energy, the device enters Sleep
Mode after standing by for more than 60 seconds.
Press to wake up the device.
4. The refractometer will turn off after 90 seconds of nonuse.
TROUBLESHOOTING
Zero setting error (Fig 7.1)
– Out of calibration (zero set) range.
Action needed: Make sure there is a sufficient
amount of clean calibration water to cover the
prism surface.
Measurement Errors
– High – exceeds the specified measurement range
(Fig 7.2).
– Low – falls below the specified measurement range
(Fig 7.3).
– Error – no liquid on the prism or not sufficient
amount of liquid (Fig 7.4).
Temperature errors
– High – temperature exceeds range (Fig 7.5).
Action needed: Check temperatures of the sample
liquid, the device, and the ambient surroundings.
– Low – temperature falls under range (Fig 7.6).
Action needed: Check temperatures of the sample
liquid, the device, and the ambient surroundings.
Battery Error
– Low – battery indication (Fig 7.7).
Action needed: Replace with two new AAA batteries
when the battery icon is red.
Fig 4.1
Fig 5.1
Fig 6.1
Fig 7.1
Fig 7.2
Fig 7.3
Fig 7.4
Fig 7.5
Fig 7.6
Fig 7.7
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©2017 Cole-Parmer Instrument Company, LLC.
1065Y5_MAN_Digital Refractometers
MAINTENANCE
WARNING
Failure to follow these precautions will void the warranty and may
cause instrument damage or inaccurate readings.
Please keep the measuring surface clean. It is extremely important to
thoroughly clean the measuring surface after each use with a wet, soft,
clean cloth or paper or towel, dampened with a mild liquid dish soap
and water. This may prevent cross-contamination between samples and
provide accurate subsequent readings.
Note: The use of solvents or petroleum-based cleaners is not
recommended.
Important Precautions
– To ensure the LCD displays properly, don't expose the instrument to
an environment with too low or too high temperature or prolonged
exposure of strong direct sunlight.
– The instrument should be protected against jarring or violent shocks.
– Do not disassemble the instrument or change the inner parts.
– Calibration should be implemented strictly according to the manual.
– Be sure to clean the prism surface and window of stage before and
after every measurement.
– To avoid having the accuracy become affected by evaporation, be
sure to perform measurement immediately after dripping solution on
window of stage.
– It may cause wrong result if keep measuring under low voltage.
– Do not use the instrument in a humid and/or corrosive environment.
– During the measurement, avoid strong light (sunlight, lamp, etc.).
– When storing the instrument for long periods of time, it is advisable
to remove the batteries. Use only AAA batteries. Pay close attention to
battery polarity when inserting batteries; reversing the polarity can
cause instrument damage.
Warranty
This instrument is warrantied against defects in materials and work-
manship for a period of one (1) year from the date of invoice. For
claims under the warranty, please contact
Cole-Parmer. The warranty does not cover fair wear and tear of parts
or accessories, nor does it apply to improper use, abnormal operation
or insufficient maintenance which is not in accordance with the
instructions in this user manual.
Repairs/Return Delivery
Please contact Cole-Parmer for troubleshooting, repairs, or returns. If
possible, use original packaging when returning the instrument to
prevent any damage during shipment.
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