Cole Parmer Jenway 7415 NANO User manual

Spectrophotometer
7415 NANO
Instruction Manual
JEN0003 /Version 1.1

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Contents
Section 1 - Introduction ............................................................................................... 7
1.1 General Description................................................................................................................. 7
1.2 Important Safety Advice .......................................................................................................... 7
1.3 SymbolsDened ..................................................................................................................... 7
1.4 Electrical Requirements............................................................................................................ 8
Section 2 - Installation................................................................................................. 9
2.1 Unpacking............................................................................................................................... 9
2.2 Installation Conditions............................................................................................................. 9
2.3 Overview................................................................................................................................. 10
2.3.1 Main View ........................................................................................................................ 10
2.3.2 Rear View ......................................................................................................................... 10
Section 3 - Theory and Practice of Spectroscopy Measurement............................... 11
3.1 Theory of Spectroscopy Measurement ..................................................................................... 11
3.2 Spectroscopy Measurement..................................................................................................... 12
3.3 Good Practice Guidelines......................................................................................................... 13
Section 4 - Instrument Set up...................................................................................... 14
4.1 Start up Screen........................................................................................................................ 14
4.2 Navigation............................................................................................................................... 14
4.3 Methods ................................................................................................................................. 15
4.4 Results .................................................................................................................................... 16
4.5 Settings................................................................................................................................... 16
4.5.1 Instrument Status.............................................................................................................. 16
4.5.2 Measurement Settings ...................................................................................................... 16
4.5.3 Network Connections ....................................................................................................... 17
4.5.4 Storage............................................................................................................................. 17
4.5.5 Regional Settings .............................................................................................................. 18
4.5.5.1 Setting the Time......................................................................................................... 18
4.5.5.2 Setting the Date ......................................................................................................... 19
4.5.5.3 Setting the Time Zone ................................................................................................ 19
4.5.5.4 Instrument Language.................................................................................................. 20
4.5.6 Service Settings................................................................................................................. 20
Section 5 - Micro Volume Settings.............................................................................. 21
Section 6 - Adding, Removing and Recovering Samples .......................................... 22
6.1 Adding a Sample..................................................................................................................... 22
6.2 Removing a Sample................................................................................................................. 22
6.3 Recovering a Sample ............................................................................................................... 22

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Section 7 - Photometrics.............................................................................................. 23
7.1 Method Set up ........................................................................................................................ 23
7.1.1 Selecting a Wavelength..................................................................................................... 23
7.2 Blank Measurement................................................................................................................. 24
7.3 Sample Measurement.............................................................................................................. 24
Section 8 - Concentration............................................................................................ 25
8.1 Method Set up ........................................................................................................................ 25
8.1.1 Selecting a Wavelength..................................................................................................... 25
8.1.2 Using a Factor................................................................................................................... 25
8.1.3 Using a Standard............................................................................................................... 26
8.1.4 Selecting Concentration Units ........................................................................................... 26
8.2 Blank Measurement................................................................................................................. 26
8.2.1 Calibrating to a Factor....................................................................................................... 27
8.2.2 Calibrating to a Standard .................................................................................................. 27
8.3 Sample Measurement.............................................................................................................. 28
8.3.1 Measuring a Sample After Calibrating to a Factor.............................................................. 28
8.3.2 Measuring a Sample After Calibrating to a Standard ......................................................... 28
Section 9 - Spectrum.................................................................................................... 29
9.1 Method Set up ........................................................................................................................ 29
9.1.1 Setting Start and End Wavelengths ................................................................................... 29
9.1.2 Setting the Scan Interval.................................................................................................... 30
9.1.3 Selecting Absorbance or % Transmittance......................................................................... 30
9.2 Blank Measurement................................................................................................................. 30
9.3 Sample Measurement.............................................................................................................. 30
9.4 Data Analysis........................................................................................................................... 31
9.4.1 Peaks and Valleys .............................................................................................................. 31
9.4.2 Area Under Curve ............................................................................................................. 32
9.4.2.1 Area Under Curve - Baseline Mode ............................................................................. 32
9.4.2.2 Area Under Curve - Tangent Mode ............................................................................. 33
Section 10 - Quantitation ............................................................................................ 34
10.1 Method Set up ........................................................................................................................ 34
10.1.1 Selecting a Wavelength..................................................................................................... 34
10.1.2 Selecting Number of Replicates ......................................................................................... 35
10.1.3 Selecting Concentration Units ........................................................................................... 35
10.2 Measuring Calibration Standards............................................................................................. 36
10.3 Standard Curve ....................................................................................................................... 37
10.4 Sample Measurement.............................................................................................................. 38

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Section 11 - Kinetics..................................................................................................... 39
11.1 Method Set up ........................................................................................................................ 39
11.1.1 Selecting a Wavelength..................................................................................................... 39
11.1.2 Setting the Kinetics Measurement Time............................................................................. 40
11.1.3 Setting the Measurement Time Interval ............................................................................. 40
11.1.4 Setting Lag Time............................................................................................................... 40
11.1.5 Selecting Absorbance or % Transmittance......................................................................... 40
11.1.6 End Point Concentration ................................................................................................... 40
11.2 Blank Measurement................................................................................................................. 41
11.3 Sample Measurement.............................................................................................................. 41
11.4 Data Analysis........................................................................................................................... 43
Section 12 - Multi-Wavelength ................................................................................... 44
12.1 Method Set up ........................................................................................................................ 44
12.1.1 Selecting a Wavelength..................................................................................................... 44
12.1.2 Equation Parameters ......................................................................................................... 45
12.1.2.1 Entering a Factor ........................................................................................................ 46
12.1.2.2 Selecting Concentration Units..................................................................................... 46
12.1.3 Selecting Absorbance or % Transmittance......................................................................... 46
12.2 Blank Measurement................................................................................................................. 47
12.3 Sample Measurement.............................................................................................................. 47
Section 13 - Nucleic Acid Modes ................................................................................. 48
13.1 dsDNA .................................................................................................................................... 48
13.2 ssDNA ..................................................................................................................................... 48
13.3 RNA ........................................................................................................................................ 48
13.4 Method Set up ........................................................................................................................ 49
13.4.1 Selecting a Wavelength..................................................................................................... 49
13.4.2 Equation Parameters ......................................................................................................... 49
13.4.2.1 Entering a Dilution Volume and Sample Volume.......................................................... 50
13.4.2.2 Entering a Factor ........................................................................................................ 50
13.5 Blank Measurement................................................................................................................. 51
13.6 Sample Measurement.............................................................................................................. 51
Section 14 - Protein Modes ......................................................................................... 52
14.1 Direct UV................................................................................................................................. 52
14.2 Warburg-Christian................................................................................................................... 52
14.3 Method Set up ........................................................................................................................ 53
14.3.1 Selecting a Wavelength..................................................................................................... 53
14.3.2 Equation Parameters ......................................................................................................... 53
14.3.2.1 Entering a Dilution Volume and Sample Volume.......................................................... 53

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14.3.2.2 Entering a Factor ........................................................................................................ 53
14.4 Blank Measurement................................................................................................................. 54
14.5 Sample Measurement.............................................................................................................. 54
Section 15 - Colorimetric Protein Assays.................................................................... 55
15.1 BCA ........................................................................................................................................ 55
15.2 Biuret ...................................................................................................................................... 55
15.3 Bradford.................................................................................................................................. 55
15.4 Lowry...................................................................................................................................... 55
15.5 Pierce 660 ............................................................................................................................... 55
15.6 Method Set up ........................................................................................................................ 56
15.6.1 Selecting a Wavelength..................................................................................................... 56
15.6.2 Selecting Number of Replicates ......................................................................................... 56
15.6.3 Selecting Concentration Units ........................................................................................... 57
15.7 Measuring Calibration Standards............................................................................................. 57
Before the 1st standard can be measured you will need to perform a blank measurement. Open the.......... 58
15.8 Standard Curve ....................................................................................................................... 59
15.9 Sample Measurement.............................................................................................................. 60
Section 16 - Saving, Loading, Deleting and Printing................................................. 61
16.1 Saving Methods ...................................................................................................................... 61
16.1.1 Saving Methods to Internal Memory ................................................................................. 61
16.1.2 Saving Methods to CPLive................................................................................................. 61
16.1.3 Saving Methods to USB Memory Stick............................................................................... 61
16.2 Loading Methods .................................................................................................................... 61
16.2.1 Loading Methods from Internal Memory ........................................................................... 61
16.2.2 Loading Methods from USB Memory Stick ........................................................................ 61
16.3 Deleting Methods.................................................................................................................... 61
16.4 Saving Results ......................................................................................................................... 62
16.4.1 Saving Results to Internal Memory .................................................................................... 62
16.4.2 Saving Results to CPLive.................................................................................................... 62
16.4.3 Saving Results to USB Memory Stick.................................................................................. 62
16.5 Loading Results ....................................................................................................................... 62
16.5.1 Loading Results from Internal Memory .............................................................................. 62
16.5.2 Loading Results from USB Memory Stick ........................................................................... 62
16.6 Deleting Results....................................................................................................................... 62
16.7 Printing ................................................................................................................................... 62
Section 17 - Accessories and Spare Parts ................................................................... 63
17.1 Optional Accessories ............................................................................................................... 63

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17.2 Spare Parts .............................................................................................................................. 63
Section 18 - Maintenance and Servicing .................................................................... 63
18.1 Routine Maintenance .............................................................................................................. 63
18.1.1 Cleaning ........................................................................................................................... 63
18.1.2 Read Head Cleaning ......................................................................................................... 63
18.1.3 Read Head Re-conditioning............................................................................................... 64
18.1.4 In Case of Accidental Spillage............................................................................................ 64
18.1.5 In Case of Contamination ................................................................................................. 64
18.2 Service, Repairs and Support ................................................................................................... 65
18.2.1 Xenon Lamp Module Replacement.................................................................................... 65
18.3 Warranty................................................................................................................................. 65
Section 19 - Environmental Protection....................................................................... 65
19.1 Packaging Material.................................................................................................................. 65
19.2 Waste Electrical and Electronic Equipment Directive (WEEE) ..................................................... 65
Section 20 - Calibration ............................................................................................... 66
20.1 Calibration Solutions ............................................................................................................... 66
20.2 Calibration Procedure.............................................................................................................. 66
20.2.1 Step 1 - Air ....................................................................................................................... 67
20.2.2 Step 2 - Blank ................................................................................................................... 67
20.2.3 Step 3 - Standard 1........................................................................................................... 68
20.2.4 Step 4 - Standard 2........................................................................................................... 68
20.2.5 Step5-Verication:Air .................................................................................................... 68
20.2.6 Step6-Verication:Blank ................................................................................................ 69
20.2.7 Step7-Verication:Standard........................................................................................... 69
Section 21 - Technical Specication ............................................................................ 70
21.1 GeneralSpecication............................................................................................................... 70
21.2 Weights and Dimensions ......................................................................................................... 71
Section 22 - Troubleshooting ...................................................................................... 72
Section 23 - Glossary of Icons ..................................................................................... 74
Section 24 - Chemical Compatability.......................................................................... 75
Index ............................................................................................................................. 76
Declaration of Conformity .......................................................................................... 79

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Section 1 - Introduction
Thank you for purchasing this Jenway product. To get the best performance from the equipment, and
for your own safety, please read these instructions carefully before use.
If the equipment is not used in the manner described in this manual and with accessories other than
those recommended by the manufacturer, the protection provided may be impaired.
1.1 General Description
The 7415 Nano is a UV/visible spectrophotometer dedicated to life science analysis. This spectrophotometer
incorporatesamicrovolume samplemeasurementaccessorythatallowssample volumesaslowas0.5μltobe
analysed. In addition to the standard measurement modes: Photometrics, Concentration, Spectrum,
Multi-wavelength, Quantitation and Kinetics, the 7415 Nano spectrophotometer is pre-programmed with methods
to determine Nucleic acid concentration and purity ratios using wavelengths recorded at 260 and 280nm, with an
optionalcorrectionat320nm.Inadditiontherearevepre-programmedmethodsforproteinanalysisincludingthe
Bradford, Lowry, Biuret, BCA and Pierce 660 as well as Direct UV and Warburg-Christian methods. This model is
compatible with CPLive.
1.2 Important Safety Advice
Usersshouldbeawareofthefollowingsafetyadvice:
❖SHOCK HAZARDS OR UNSAFE PRACTICES ARE DANGEROUSastheycancauseseverepersonalinjury,re
or death.
❖DO NOT use combustible substances near hot objects.
❖DO NOT use the equipment in hazardous atmospheres.
❖DO NOToperateorhandleanypartoftheequipmentwithwethandsoruseonsurfacesthatmaybecomeooded.
❖NEVER move the equipment while still connected to the power supply.
❖HIGH TEMPERATURES ARE DANGEROUS as they can cause serious burns to operators and ignite combustible
material.
❖USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS.
❖NEVER lift or carry the equipment during operation.
❖ DO NOTpositiontheequipmentsothatitisdifculttodisconnectfromthemainssupplyusingthemains
plug.
❖ Themainsoutletsocketusedshouldbelocatedclosetotheequipmentandreadilyidentiableandaccessible
to users.
❖DO NOT leave equipment switched on and it is not recommended to leave any heating apparatus unattended
during operation.
❖The equipment should be carried using both hands.
1.3 Symbols Dened
WARNINGEARTH RISK OF
ELECTRIC
SHOCK
BIOHAZARD HOT
SURFACE
INFORMATION UV LIGHT
SOURCE

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1.4 Electrical Requirements
THIS INSTRUMENT MUST BE GROUNDED
Before connection please ensure that the line supply corresponds to the power requirements below:
Power Supply requirements
65 W 100 V - 230 V ~ 50/60 Hz
The equipment is provided with a power supply unit and three power cables consisting of a UK 3-pin and a “Schuko”
2-pin plug for 230 V installations and a NEMA 5-15 plug for 120 V installations.
Choose the power cable appropriate for your electrical installation and discard the others. Should one of the power
cables not be suitable for connecting to the power supply, replace the plug with a suitable alternative.
THIS OPERATION SHOULD ONLY BE UNDERTAKEN BY A QUALIFIED ELECTRICIAN.
NOTE: Refer to the equipment rating plate to ensure that the plug and fusing are suitable for the voltage and
wattagestated.Thewiresinthemainscableareasfollows:
230 V a.c. 120 V a.c.
HOT/LIVE - BROWN BLACK - HOT/LIVE
NEUTRAL - BLUE WHITE - NEUTRAL
EARTH - GREEN/YELLOW GREEN - EARTH
The appropriate power cable and power adaptor combination should be connected to the equipment BEFORE
connection to the mains supply. Should the mains lead require replacement, a cable of 1.25mm² (AWG16) of
harmonised code H05VV-F connected to an IEC320 plug should be used.
IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN

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Section 2 - Installation
2.1 Unpacking
Before discarding the packaging check
that all parts are present and correct.
17415 Nano
2Instruction manual
3UK power lead
4EU power lead
5US power lead
6Power supply unit
2.2 Installation Conditions
Whentheequipmentisusedforthersttimeormovedtoadifferentenvironmentaltemperature,itis
important to allow the equipment to equalise to the ambient temperature. We recommend you allow
the equipment to stand for 2 hours before switching on.
Thisequipmentisdesignedtooperatesafelyunderthefollowingconditions:
❖For indoor use only
❖Use in a well ventilated area
❖Ambient temperature range 5°C to 40°C (41°F to 104°F)*
*Best results are achieved when the equipment is operated within a temperature range of 15ºC to 35ºC
(59ºF to 95ºF).
❖Altitude to 2000m (6500 ft)
❖Relative humidity not exceeding 80% (temperature 31ºC) decreasing to 50% (temperature 40ºC) and free
from condensation
❖Mainssupplyuctuationsnotexceeding10%ofnominal
❖Overvoltage category II IEC60364-4-443
❖Pollution degree 2 IEC664
❖Use with a minimum distance all round of 300mm (12in.) from walls or other items
Placetheequipmentonaclean,rm,levelsurfacewhichisfreefromdrafts.Avoidinstallationonaslipperysurface
oronasurfacepronetovibrationoronasurfacepronetoooding.
Select the power lead and attach to the power supply unit. Connect the power supply unit to the power inlet
socket on the rear panel of the instrument and connect to the mains socket. Ensure that the sample chamber is
empty and all packaging material has been removed before turning the power on at the mains and switching the
instrument on using the power switch on the side of the instrument.
The equipment will perform several power-on tests and wavelength calibration before displaying the main screen.
3 4 5
1 2
6

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2.3 Overview
2.3.1 Main View
1Lid 42 x USB Type A ports
2Open and close catch 5On/Off power switch
3Colour touchscreen and user interface
2.3.2 Rear View
6Ethernet (RJ45) port
7USB Type B port
8Power inlet socket
1
2
3
4
5
6
7
8

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Section 3 - Theory and Practice of Spectroscopy Measurement
3.1 Theory of Spectroscopy Measurement
UV-visiblespectroscopyisthemeasurementoftheabsorbanceoflightataspecicwavelengthinasample.Thisis
used to identify the presence and concentration of molecular entities within the sample. The Beer-Lambert law is
used to relate the absorption of light to the properties of the sample through which the light is travelling through.
TheBeer-Lambertlawstatesthat:
A = Ɛ l c
A is the absorbance
Ɛ isthemolarabsorptioncoefcient(lmol-1cm-1)
lis the path length (cm)
c is the concentration (mol I-1)
This law shows that absorbance is linear to concentration but this is only true for low concentrations. For
absorbance levels above 3 the concentration starts to move away from the linear relationship.
Transmittanceistheproportionofthelightwhichpassesthroughthesample:
Where:
lois the incident light
ltis the transmitted light
lis the path length
Therefore:
Absorbanceisinverselyrelatedtotransmittance:
NOTE:The7415Nanodoesnotusexedpathlengthcuvettes.Adropletofsampleisplacedonthereadhead
and a path length of either 0.2mm or 0.5mm is chosen by the user, see Section 5 - Micro Volume Settings for more
information.
Io
I
It
Io
It
T =
T
Log 1
A =
Io
I
It
Io
I
t
T =
T
Log 1
A =
Io
I
It
Io
It
T =
T
Log 1
A =

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3.2 Spectroscopy Measurement
There are four main components of a spectrophotometer. These are a light source to emit a high and constant
amount of energy over the full wavelength range; a method for separating the light into discreet wavelengths; a
sample holder and a light detector.
Theopticallayoutofthe7415Nanospectrophotometerisshownbelow:
Thelight fromthe pre-alignedxenon lamp is focused throughabreopticcable at 1200lines per millimetre,
whichseparatesthelightintodiscretewavelengths.Tworeadheadsareconnectedbybreopticcablestothe
light source and detector. A droplet of sample (2-5µl) is placed on the lower read head. The upper read head is
thenloweredtoadenedpathlength,either0.5mmor0.2mm.Thedefractedlightispassedthroughthesample
and the absorbance measured.
Reducing the path length results in a lower absorbance value than with a standard 10mm cuvette, so although
smaller volumes can be used, the concentration of the sample needs to be higher to achieve a readable absorbance.
The “virtual dilution” is 20-fold at 0.5mm and 50-fold at 0.2mm.
Light from Xenon lamp
Light direction
Sample droplet
Lower read head
Upper read head
Detected by photodiode detector
Fibre optic cables

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3.3 Good Practice Guidelines
1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere.
When in use ambient temperature and light levels should remain as constant as possible.
2. If required, adherence to Standard Operating Procedures (S.O.P.) and Good Laboratory Practice (G.L.P.) should be
monitored with regular calibration checks and a suitable Quality Control (Q.C.) programme.
3. The sample chamber lid must be fully closed during measurement before any readings are recorded or
printed.
4. All measurements require calibration to a blank, for maximum accuracy this should be prepared with care
using the same deionised water or solvent used for dissolving or diluting the sample. Where reagents are
added to the sample to produce a colour proportional to its concentration a ‘sample based’ blank should be
used. In this case the blank should consist of all reagents or chemicals to be used, except the sample which
will produce the colour to be measured.
5. Ensure the read heads are clean. Wipe both the upper and lower read heads with a lint free cloth wetted with
deionised water to remove any residues of previous samples. Dry with a fresh cloth.
6. If a stable droplet does not form, “buff” the read head surfaces by rubbing rmly with a dry laboratory
wipe 30-40 times. This will “re-condition” the surface.
7. Make sure that the sample is well mixed and free of air bubbles or particles. If a bubble is created when
pipetting the sample, remove the sample and repeat the application.
8. Ifpossibleuseatleast2μlofsampleformeasurement.Whenmeasuringat0.2mmpathlength,aminimum
of0.5μlcanbeused.
9. Read each sample droplet only once. The read head moves into a default position after the sample has been
measured. This means that if the sample is measured a second time, contact of the droplet with the read heads
could be lost and the subsequent reading will not give a valid result.
10. Useasampleof sufcientconcentration.Rememberthat the short pathlengthcreatesa “virtualdilution”
of the sample of 1 in 20 at 0.5mm and 1 in 50 at 0.2mm. This means that a sample which would normally read
an absorbance of 1.0 in a standard 10mm cuvette will only give a value of 0.05 at 0.5mm or 0.02 at 0.2mm.
11. To minimise any factors which may interfere with a reading such as sample turbidity or contaminants carried
over from sample preparation, it is recommended that a background reading is also made at a second reference
wavelength (where the absorbance of the sample is very low and unchanging).
12. Use the same measurement mode if comparing the concentrations of samples. Different modes use different
equationstocalculatethenalsampleconcentration.
13. Be aware that when measuring micro volume samples, very small changes in absorbance can lead to much
greater differences in calculated concentration values due to the inherent “dilution”factor of the small path
length. For example, when measuring dsDNA, an absorbance change of just 0.001 equates to a derived
concentrationchangeof1μg/mlat0.5mmpathlength(basedon1A260unitofdsDNA=50μg/ml).
14. Jenway recommend that the micro volume accessory is calibrated every 6 months. A set of calibration
solutions is available to order (part code 035 092). See Section 20 - Calibration for more information.

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Section 4 - Instrument Set up
4.1 Start up Screen
Thepowerupscreenisshownbelow:
4.2 Navigation
Themainmenuisshownbelow:
This spectrophotometer is controlled solely through the touchscreen interface of the equipment and follows a
basic Android user interface. If the number of options available in a menu exceeds the number that can be
displayed on the screen, swipe to the left to view the other modes.
The main menu screen provides access to all Measurement modes, Methods, Results and the Settings menu.
Additional icons are displayed when the unit is connected to a network and if an active accessory is installed.
The settings menu enables access to Instrument status, Measurement settings, Network connections
Storage, Regional settings and Service settings.

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Throughout,thesoftwareoptionscanbeturnedONandOFFusingaswitch:
Option is ON
Option is OFF
Ineachmeasurementmodethereisanoverowicon giving additional save and load method options.
Touching gives options to load a previously saved method, touch to save the
entered method parameters, touch to upload to CPLive* or touch to
clear recently used method parameters. *Refer to CPLive instructions for more information.
When required to enter numbers, a keypad will pop up. Touch the required numbers and touch to apply. To
exit the keypad without changing the entered value touch or the minimize icon .
When required to enter letters, a keypad will pop up. Touch the required letters and touch Save to apply. To
exit the keypad without changing the entered value touch Save or the minimize icon .
4.3 Methods
Touch to access methods that have been saved. Touch the required method to view the details of the
method set up. You will then be able to delete, upload, export, edit or run the selected method. See Section 16 -
Saving, Loading, Deleting and Printing for more information.
Save

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4.4 Results
Touch to access results that have been saved. Touch the required results to view the details of the
result. You will then be able to delete, upload or export the selected result. See Section 16 for more information.
4.5 Settings
Touch to enable access to instrument status, measurement settings, network connections, storage
and service settings.
4.5.1 Instrument Status
Touch  to view the status of the spectrophotometer, check connections, tted accessories,
the serial number of the unit and the software version the instrument is using. The language and date and time
can also be set here.
4.5.2 Measurement Settings
Touch to select options for autosave results and the favourites panel.
Slide the button to the position to automatically save results to the instruments internal memory.
Slide the button to the position to view favourite panel on the home screen.
Touch to choose Celsius or Fahrenheit
Slide the button to the position to send anonymous diagnostic and feedback data to
Cole-Parmertohelpxandimprovesoftwareandservices.

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4.5.3 Network Connections
Touch to view available network connections. Options include Ethernet (RJ45), IP
conguration,Status,IPaddressandCPLiveoptions.
4.5.4 Storage
Touch to view the amount of available storage on the internal memory of the spectrophotometer. If a
USB memory stick is inserted the amount of free space on the USB stick will also be shown.

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4.5.5 Regional Settings
Touch to view the Time, Date, Time zone and Language options.
4.5.5.1 Setting the Time
To set the instrument time touch . Touch and move the clock hand to the correct hour position,
repeat the same process for minutes, select AM or PM and touch to apply. Touching will return to the
instrument settings screen without altering the time. The set time will be displayed in each measurement
mode and will be recorded against saved methods and results.

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4.5.5.2 Setting the Date
To set the instrument date touch . Scroll up or down to change the month viewed. Touch the
required date and touch to apply. To set the year touch and scroll up or down and touch the
required date and touch to apply. Touching will return to the instrument settings screen
without altering the date. The set date will be displayed in each measurement mode and will be recorded against
saved methods and results.
4.5.5.3 Setting the Time Zone
To set the instrument time zone touch . Scroll up or down to locate the required time
zone and touch next to the required time zone to apply.

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4.5.5.4 Instrument Language
The software can be viewed in ve different languages with a choice of English, French, German, Italian or
Spanish. To select the required language touch and select from the menu. Touch next to the
required language to apply.
4.5.6 Service Settings
Service Settings are protected from normal use. They must only be accessed by service engineers only.