Cole Parmer Jenway 7415 User manual

Spectrophotometer
7410 and 7415
Instruction Manual
JEN0001 / Version 1.0

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Contents
Section 1 - Introduction............................................................................................... 6
1.1 General Description................................................................................................................. 6
1.2 Important Safety Advice .......................................................................................................... 6
1.3 SymbolsDened ..................................................................................................................... 6
1.4 Electrical Requirements............................................................................................................ 7
Section 2 - Installation................................................................................................. 8
2.1 Unpacking............................................................................................................................... 8
2.2 Installation Conditions............................................................................................................. 8
2.3 Overview................................................................................................................................. 9
Section 3 - Theory and Practice of Spectroscopy Measurement............................... 10
3.1 Theory of Spectroscopy Measurement ..................................................................................... 10
3.2 Spectroscopy Measurement..................................................................................................... 11
3.3 Good Practice Guidelines......................................................................................................... 12
Section 4 - Instrument Set up...................................................................................... 14
4.1 Start up Screen........................................................................................................................ 14
4.2 Navigation............................................................................................................................... 14
4.3 Methods ................................................................................................................................. 16
4.4 Results .................................................................................................................................... 16
4.5 Settings................................................................................................................................... 16
4.5.1 Instrument Status.............................................................................................................. 16
4.5.1.1 Instrument Language.................................................................................................. 16
4.5.1.2 Setting the Time......................................................................................................... 17
4.5.1.3 Setting the Date ......................................................................................................... 17
4.5.1.4 Setting the Time Zone ................................................................................................ 18
4.5.2 Measurement Settings ...................................................................................................... 18
4.5.3 Network Connections ....................................................................................................... 19
4.5.4 Storage............................................................................................................................. 19
4.5.5 Service Settings................................................................................................................. 19
Section 5 - Photometrics.............................................................................................. 20
5.1 Method Set up ........................................................................................................................ 20
5.1.1 Selecting a Wavelength..................................................................................................... 20
5.2 Calibration .............................................................................................................................. 21
5.3 Sample Measurement.............................................................................................................. 21
Section 6 - Concentration............................................................................................ 22
6.1 Method Set up ........................................................................................................................ 22
6.1.1 Selecting a Wavelength..................................................................................................... 22

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6.1.2 Using a Factor................................................................................................................... 22
6.1.3 Using a Standard............................................................................................................... 23
6.1.4 Selecting Concentration Units ........................................................................................... 23
6.2 Calibration .............................................................................................................................. 23
6.2.1 Calibrating to a Factor....................................................................................................... 24
6.2.2 Calibrating to a Standard .................................................................................................. 24
6.3 Sample Measurement.............................................................................................................. 25
6.3.1 Measuring a Sample After Calibrating to a Factor.............................................................. 25
6.3.2 Measuring a Sample After Calibrating to a Standard ......................................................... 25
Section 7 - Spectrum.................................................................................................... 26
7.1 Method Set up ........................................................................................................................ 26
7.1.1 Setting Start and End Wavelengths ................................................................................... 26
7.1.2 Setting the Scan Interval.................................................................................................... 27
7.1.3 Selecting Absorbance or % Transmittance......................................................................... 27
7.2 Calibration .............................................................................................................................. 27
7.3 Sample Measurement.............................................................................................................. 27
7.4 Data Analysis........................................................................................................................... 28
7.4.1 Peaks and Valleys .............................................................................................................. 28
7.4.2 Area Under Curve ............................................................................................................. 29
7.4.2.1 Area Under Curve - Baseline Mode ............................................................................. 29
7.4.2.2 Area Under Curve - Tangent Mode ............................................................................. 30
Section 8 - Quantitation .............................................................................................. 31
8.1 Method Set up ........................................................................................................................ 31
8.1.1 Selecting a Wavelength..................................................................................................... 31
8.1.2 Selecting Number of Replicates ......................................................................................... 32
8.1.3 Selecting Concentration Units ........................................................................................... 32
8.2 Measuring Calibration Standards............................................................................................. 33
8.3 Standard Curve ....................................................................................................................... 34
8.4 Sample Measurement.............................................................................................................. 35
Section 9 - Kinetics....................................................................................................... 36
9.1 Method Set up ........................................................................................................................ 36
9.1.1 Selecting a Wavelength..................................................................................................... 36
9.1.2 Setting the Kinetics Measurement Time............................................................................. 37
9.1.3 Setting the Measurement Time Interval ............................................................................. 37
9.1.4 Setting Lag Time............................................................................................................... 37
9.1.5 Selecting Absorbance or % Transmittance......................................................................... 37
9.1.6 End Point Concentration ................................................................................................... 37
9.2 Calibration .............................................................................................................................. 38

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9.3 Sample Measurement.............................................................................................................. 38
9.4 Data Analysis........................................................................................................................... 40
Section 10 - Multi-Wavelength ................................................................................... 41
10.1 Method Set up ........................................................................................................................ 41
10.1.1 Selecting a Wavelength..................................................................................................... 41
10.1.2 Equation Parameters ......................................................................................................... 42
10.1.2.1 Entering a Factor ........................................................................................................ 43
10.1.2.2 Selecting Concentration Units..................................................................................... 43
10.1.3 Selecting Absorbance or % Transmittance......................................................................... 43
10.2 Calibration .............................................................................................................................. 44
10.3 Sample Measurement.............................................................................................................. 44
Section 11 - Saving, Loading, Deleting and Printing................................................. 45
11.1 Saving Methods ...................................................................................................................... 45
11.1.1 Saving Methods to Internal Memory ................................................................................. 45
11.1.2 Saving Methods to CPLive................................................................................................. 45
11.1.3 Saving Methods to USB Memory Stick............................................................................... 45
11.2 Loading Methods .................................................................................................................... 45
11.2.1 Loading Methods from Internal Memory ........................................................................... 45
11.2.2 Loading Methods from USB Memory Stick ........................................................................ 45
11.3 Deleting Methods.................................................................................................................... 45
11.4 Saving Results ......................................................................................................................... 46
11.4.1 Saving Results to Internal Memory .................................................................................... 46
11.4.2 Saving Results to CPLive.................................................................................................... 46
11.4.3 Saving Results to USB Memory Stick.................................................................................. 46
11.5 Loading Results ....................................................................................................................... 46
11.5.1 Loading Results from Internal Memory .............................................................................. 46
11.5.2 Loading Results from USB Memory Stick ........................................................................... 46
11.6 Deleting Results....................................................................................................................... 46
11.7 Printing ................................................................................................................................... 46
Section 12 - Accessories and Spare Parts ................................................................... 47
12.1 Optional Accessories ............................................................................................................... 47
12.2 Installing the Accessories ......................................................................................................... 47
12.2.1 Passive Accessories............................................................................................................ 47
12.2.2 Active Accessories............................................................................................................. 48
12.2.2.1 Installing Automatic 8 Cell Turret ................................................................................ 49
12.3 Using the Accessories .............................................................................................................. 50
12.3.1 Automatic 8 Cell Turret ..................................................................................................... 50
12.3.1.1 Automatic 8 Cell Turret - Manual Mode...................................................................... 50

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12.3.1.2 Automatic 8 Cell Turret - Automatic Mode.................................................................. 52
12.3.1.3 Automatic 8 Cell Turret - Supporting Creation of a Standard Curve in Quantitation..... 54
12.4 Spare Parts .............................................................................................................................. 54
Section 13 - Maintenance, Servicing and Cleaning ................................................... 55
13.1 Routine Maintenance .............................................................................................................. 55
13.2 Lamp Replacement.................................................................................................................. 57
13.2.1 Tungsten Halogen Lamp Replacement............................................................................... 57
13.2.2 Xenon Lamp Module Replacement.................................................................................... 58
13.3 Service, Repairs and Support ................................................................................................... 58
13.4 Warranty................................................................................................................................. 58
Section 14 - Environmental Protection....................................................................... 58
14.1 Packaging Material.................................................................................................................. 58
14.2 Waste Electrical and Electronic Equipment Directive (WEEE) ..................................................... 58
Section 15 - Technical Specication ............................................................................ 59
15.1 GeneralSpecication............................................................................................................... 59
15.2 Weights and Dimensions ......................................................................................................... 60
Section 16 - Troubleshooting ...................................................................................... 61
Section 17 - Glossary of Icons ..................................................................................... 63
Index ............................................................................................................................. 64
Declaration of Conformity .......................................................................................... 67

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Section 1 - Introduction
Thank you for purchasing this Jenway product. To get the best performance from the equipment, and
for your own safety, please read these instructions carefully before use.
If the equipment is not used in the manner described in this manual and with accessories other than
those recommended by the manufacturer, the protection provided may be impaired.
1.1 General Description
The 7410 and 7415 spectrophotometers are suited to a wide range of applications in education, quality
control, environmental and clinical analysis. The 7410 is a visible spectrophotometer covering a wavelength range
from 320nm to 1000nm. The 7415 is a UV/Visible spectrophotometer with a wavelength range from 198nm
to 1000nm. Both models have measurement modes for Photometrics, Concentration, Spectrum scanning,
Multi-wavelength, Quantitation and Kinetics. Both models are compatible with CPLive.
1.2 Important Safety Advice
Users should be aware of the following safety advice:
❖SHOCK HAZARDS OR UNSAFE PRACTICES ARE DANGEROUSastheycancauseseverepersonalinjury,re
or death.
❖DO NOT use combustible substances near hot objects.
❖DO NOT use the equipment in hazardous atmospheres.
❖DO NOToperateorhandleanypartoftheequipmentwithwethandsoruseonsurfacesthatmaybecomeooded.
❖NEVER move the equipment while still connected to the power supply.
❖HIGH TEMPERATURES ARE DANGEROUS as they can cause serious burns to operators and ignite combustible
material.
❖USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS.
❖NEVER lift or carry the equipment during operation.
❖ DO NOTpositiontheequipmentunitsothatitisdifculttodisconnectfromthemainssupplyusingthemains
plug.
❖ Themainsoutletsocketusedshouldbelocatedclosetotheequipmentandreadilyidentiableandaccessible
to users.
❖DO NOT leave equipment switched on and it is not recommended to leave any heating apparatus unattended
during operation.
❖The equipment should be carried using both hands.
1.3 Symbols Dened
WARNINGEARTH RISK OF
ELECTRIC
SHOCK
BIOHAZARD HOT
SURFACE
INFORMATION UV LIGHT
SOURCE

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1.4 Electrical Requirements
THIS INSTRUMENT MUST BE GROUNDED
Before connection please ensure that the line supply corresponds to the power requirements below:
Power Supply requirements
65 W 100 V - 230 V ~ 50/60 Hz
The equipment is provided with a power supply unit and three power cables consisting of a UK 3-pin and a “Schuko”
2-pin plug for 230 V installations and a NEMA 5-15 plug for 120 V installations.
Choose the power cable appropriate for your electrical installation and discard the others. Should one of the power
cables be suitable for connecting to the power supply, replace the plug with a suitable alternative..
THIS OPERATION SHOULD ONLY BE UNDERTAKEN BY A QUALIFIED ELECTRICIAN.
NOTE: Refer to the equipment rating plate to ensure that the plug and fusing are suitable for the voltage and
wattage stated. The wires in the mains cable are as follows:
230 V a.c. 120 V a.c.
HOT/LIVE - BROWN BLACK - HOT/LIVE
NEUTRAL - BLUE WHITE - NEUTRAL
EARTH - GREEN/YELLOW GREEN - EARTH
The appropriate power cable and power adaptor combination should be connected to the equipment BEFORE
connection to the mains supply. Should the mains lead require replacement, a cable of 1.25mm² (AWG16) of
harmonised code H05VV-F connected to an IEC320 plug should be used.
IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN

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Section 2 - Installation
2.1 Unpacking
Before discarding the packaging check
that all parts are present and correct.
1 7410 / 7415
2Instruction manual
3UK power lead
4EU power lead
5US power lead
6Power supply unit
2.2 Installation Conditions
 Whentheequipmentisusedforthersttimeormovedtoadifferentenvironmentaltemperature,itis
important to allow the equipment to equalise to the ambient temperature. We recommend you allow
the equipment to stand for 2 hours before switching on.
This equipment is designed to operate safely under the following conditions:
❖ For indoor use only
❖ Use in a well ventilated area
❖ Ambient temperature range 5°C to 40°C (41°F to 104°F)
❖ Altitude to 2000m (6500 ft)
❖ Relative humidity not exceeding 80% (temperature 31ºC) decreasing to 50% (temperature 40ºC) and free
from condensation
❖ Mainssupplyuctuationsnotexceeding10%ofnominal
❖ Overvoltage category II IEC60364-4-443
❖ Pollution degree 2 IEC664
❖ Use with a minimum distance all round of 300mm (12in.) from walls or other items
Placetheequipmentonaclean,rm,levelsurfacewhichisfreefromdrafts.Avoidinstallationonaslipperysurface
oronasurfacepronetovibrationoronasurfacepronetoooding.
Select the power lead and attach to the power supply unit. Connect the power supply unit to the power inlet
socket on the rear panel of the instrument and connect to the mains socket. Ensure that the sample chamber is
empty before turning the power on at the mains and switching the instrument on using the power switch on the
side of the instrument.
The equipment will perform several power-on tests and wavelength calibration before displaying the main screen.
NOTE: 7410 must be switched on 30 minutes before any readings are performed to allow the tungsten lamp to
warm up
NOTE: Leaving cuvettes in the sample holder during power up will result in failure of the power on tests.
345
1 2
6

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2.3 Overview
Main View
1 Lid
2 Open and close catch
3 Colour touchscreen
and user interface
4 Removable panel
for sipper accessory
5 2 x USB Type A ports
6 On/Off power switch
Rear View
7 Ethernet (RJ-45) port
8 USB Type B port
9 Power inlet socket
Underneath View
10 Lamp holder cover
Refer to Section 13.2 for instructions on how to remove and replace the lamp.
1
2
3
4
10
5
6
7
4
8
9

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Section 3 - Theory and Practice of Spectroscopy Measurement
3.1 Theory of Spectroscopy Measurement
UV-visiblespectroscopyisthemeasurementoftheabsorbanceoflightataspecicwavelengthinasample.Thisis
used to identify the presence and concentration of molecular entities within the sample. The Beer-Lambert law is
used to relate the absorption of light to the properties of the sample through which the light is travelling through.
The Beer-Lambert law states that:
A = Ɛ l c
A is the absorbance
Ɛ isthemolarabsorptioncoefcient(lmol-1cm-1)
l is the path length (cm)
c is the concentration (mol I-1)
This law shows that absorbance is linear to concentration but this is only true for low concentrations. For
absorbance levels above 3 the concentration starts to move away from the linear relationship.
Transmittance is the proportion of the light which passes through the sample:
Where:
lois the incident light
ltis the transmitted light
l is the path length
Therefore:
Absorbance is inversely related to transmittance:
Io
I
It
Io
It
T =
T
Log 1
A =
Io
I
It
Io
I
t
T =
T
Log 1
A =
Io
I
It
Io
It
T =
T
Log 1
A =