cytiva AKTA start Parts list manual

ÄKTA start
Maintenance Cue card
cytiva.com
Table of Contents
Flow path overview 2
Regular maintenance 2
Cleaning system ow path 3
Clean the column 6
Other maintenance procedures 6
Storage of the instrument 11
Getting help 12
Reference information 12
Safety precautions 12
Overview
ÄKTA™ start is a simple and modern liquid chromatography system intended for preparative purication of
proteins at laboratory scale. The system can be used for a variety of research purposes to fulll the needs of
the users in academia and the life sciences industry.
The Maintenance Cue Card describes the maintenance schedule for ÄKTA start and Frac30 (the Fraction
collector), and provides instructions for routine maintenance.
Regular maintenance is important for safe and trouble-free operation of ÄKTA start.
The system consist of ÄKTA start instrument, a fraction collector and a PC with UNICORN™ start software.
For a detailed description of the system, refer to ÄKTA start System Cue Card and ÄKTA start Operating
Instructions.
WARNING
Read ÄKTA start Operating Instructions
before using the instrument.

ÄKTA start Maintenance Cue Card, 29024043 AF2
Flow path overview
The illustration below shows the ow path for ÄKTA
start.
Part Description Part Description Part Description
1Buffer valve 5 Pressure sensor 9 UV Monitor
2 Mixer 6 Wash valve 10 Conductivity Monitor
3Sample valve 7Injection valve (manual) 11 Outlet valve
4 Pump 8 Column 12 Fraction collector
Regular
maintenance
Regular maintenance is important for safe and
trouble-free operation of ÄKTA start. The user
should perform maintenance on a daily, weekly and
monthly basis.
Periodic maintenance schedule
The periodic maintenance schedule provides a
guide to the user to ensure proper maintenance of
the ÄKTA start and Frac30.
Interval Maintenance action
Daily Visually inspect the instrument for
leakages in the ow path.
If there are signs of liquid leaking,
check integrity of tubing and tubing
connections.
Clean the column and ow path after
use and leave the system lled with
DM water.
Note: If the instrument is not going to be
used for 1-2 days, prepare the system for
storage.
Note: Open the pump cover after
switching o the instrument.

ÄKTA start Maintenance Cue Card, 29024043 AF 3
Cleaning system ow
path
Purpose
The system methods are used for cleaning the
system ow path, including all the inlets and outlets.
The following wash methods are available:
• Pump wash A, Pump wash B
• Washout fractionation tubing
• System cleaning
Pump wash A/B
The Pump wash method is used to wash the
selected inlets/outlets and ow path tubing at high
ow rate using the specied buffer port. Pump
wash prevents cross-contamination and carryover
of buffers/samples.
The Pump wash method is recommended:
• Before starting a run when the system has not
been used for a prolonged period of time
• When buffers are changed
Instruction
1. Immerse the buffer Aor Binlet tubing in DM
water or buffer of choice.
2. Follow the workow below to run Pump wash.
Interval Maintenance action
Daily
or when
required
Clean the valves (at least with DM
water) to avoid salt crystal formation
after every run or at the end of the
day, or when the instrument will be
left idle for several days.
Cleaning Procedure: DM water can
be used to clean the Buffer valve
and Wash valve by following the
Pump wash methods. Sample valve
can be cleaned using manual run.
Note: If more thorough cleaning is
required 1 M NaOH may be used. When
NaOH is used for cleaning, wash the
owpath thoroughly with DM water
before starting a run.
Weekly,
or when
required
Visually inspect the inlet lters and
clean them if necessary.
Cleaning procedure: immerse and
leave the inlet lters in 1 M NaOH for
about 2 hours.
Clean the system ow path with
0.5 to 1 M NaOH and rinse with
demineralized (DM) water.
Note: Do not leave NaOH in the system
for more than 20 min.
Note: Cleaning may be necessary more
or less frequently, depending on the
system usage and the nature of the
samples.
Calibrate the Pump.
When
required
Clean the instrument externally.
Cleaning procedure: Wipe the
surface with a damp cloth. Wipe off
stains using a mild cleaning agent or
20% ethanol.
Clean the Fraction collector.
Note: Frac30 is not a spill-free design.
There will be some spillage when tubes
shift position, depending on the ow rate.
Perform System cleaning,
Pump Wash A and B, Washout
Fractionation tubing.
Clean the UV ow cell.
Clean the Conductivity cell.
Calibrate UV.
Calibrate Conductivity.
Interval Maintenance action
Perform Zero offset for the Pressure
sensor.
Replace the inlet lters.
Replace the tubing and connectors.
Yearly,
or when
required
Check the drive sleeve on the
Fraction collector. Replace if worn
out.

ÄKTA start Maintenance Cue Card, 29024043 AF4
Operation from the Instrument
Display
Perform the following operations to run Washout
fractionation tubing.
Method run
Prepare system
Washout fractionation
tubing
EndRun
Exit
Edit run
Set Run parameters to
suit your requirement
ÄKTA start
Operation from UNICORN start
Perform the following operations to run Washout
fractionation tubing.
UNICORN start
Method run
System control
Pre defined
System preparation
Washout fractionation
tubing
System cleaning
The System cleaning method is used to clean the
instrument ow path. The method is recommended
for:
Washout fractionation tubing
The Washout fractionation tubing method is used
to clean the fractionation tubing. The method is
recommended:
• Before collecting the fractions using the Outlet
valve without Fraction collector
• When buffers are changed
Instruction
1. Immerse the buffer inlet tubing in DM water
or the buffer of choice.
2. Disconnect the column from the ow path and
reconnect the tubing with a Union connector.
3. Follow the workow below to run Washout
fractionation tubing.
Select
method
Method run
Prepare system
Pump wash A
End
Run
Exit
Pump wash B
Immerse
inlet A
in Buffer A
Immerse
inlet B
in Buffer B
ÄKTA start
Operation from UNICORN start
Perform the following operations to run Pump wash.
UNICORN start
Method run
System control
Pre defined
System preparation
Pump wash A /
Pump wash B
Operation from the Instrument
Display
Perform the following operations to run Pump wash.

ÄKTA start Maintenance Cue Card, 29024043 AF 5
Instruction
1. Clean the inlets and outlets (buffer tubing,
sample inlet tubing, fractionation tubing)
2. Immerse the buffer inlet tubing (A & B) in 1 M
NaOH.
3. Disconnect column from the ow path and
reconnect the tubing with a Union connector.
4. Follow the workow below to run System
cleaning.
Note:
• Do not leave NaOH in the system. Wash the ow
path thoroughly with water.
• Check the pH after water wash to ensure
complete removal of NaOH.
• It is recommended not to end the method before
it is completed.
Operation from the Instrument
Display
Perform the following operations to run System
cleaning.
Method run
Prepare system
Follow the instructions
& tap Continue
Run ended
Exit
System cleaning
When prompted, immerse the
buffer inlets (A & B) in DM water
Continue
ÄKTA start
Operation from UNICORN start
Perform the following operations to run System
cleaning.
UNICORN start
Method run
System control
Pre defined
System preparation
System cleaning
Clean the Buffer valve and Wash
valve
The Buffer valve and Wash valve can be cleaned
using DM water.
Note: If a more thorough cleaning is required, 1 M NaOH
may be used. When NaOH is used for cleaning, make sure
to wash the ow path thoroughly with DM water before
starting a run.
Instruction
• Perform Pump wash B and Pump wash A
protocols (in that order) ro clean the buffer valve
and wash valve. See Section Pump wash A/B on
page 3.
Set Pump wash ow rate to 10 ml/min.
• If NaOH is used for cleaning, repeat the Pump
wash B and Pump wash A protocols with DM
water until the NaOH is completely removed.
Clean the Sample valve
The Sample valve can be cleaned using DM water.
Note: If a more thorough cleaning is required, 1 M NaOH
may be used. When NaOH is used for cleaning, make sure
to wash the ow path thoroughly with DM water before
starting a run.
Instruction
1. Immerse the sample inlet tubing in the cleaning
solution (DM water or 1 M NaOH).
2. Set Run parameters
• Set Flow rate to 5 ml/min.
• Clear the Save results to USB option.
3. Set Flow path:
• Set Sample valve to Sample.
• Set Wash valve to Column.
• Set Outlet valve to Waste.
• Routine maintenance
• Preparing the system for storage
• Removing/preventing contamination (bacterial
growth) in the ow path
• Preventing carryover of buffers/samples
Cleaning solutions
• 1 M NaOH
• DM water

ÄKTA start Maintenance Cue Card, 29024043 AF6
Manual run
Set Run parameters
Run
ÄKTA start
Set Run parameters
End Run
Set Flow path
Operation from UNICORN start
Perform the following operations to clean the
Sample valve.
System control
Set Run parameters
Run
UNICORN start
Manual run
End Run
Set Run parameters
Set Flow path
Storage
If the column is not going to be used for a couple of
days or longer, store the column.
Instruction
• Fill the column with the storage solution
recommended in the column data sheet.
• Detach the column from the instrument
and store it according to the column
recommendation.
Other maintenance
procedures
Clean the UV ow cell
Maintenance interval
Clean the UV ow cell once every three months, or
when the signal is unstable or readings appear to be
incorrect.
Cleaning solutions
• 10% surfactant detergent solution (e.g., Decon™
90, Deconex™ 11, or RBS™ 25)
• DM water
Instruction
1. Disconnect the tubing from the top of the UV
ow cell, and replace the ngertight connector
with a Luer connector (needed for connecting a
syringe).
2. Disconnect the tubing from the bottom of the UV
ow cell, connect a piece of tubing of sufcient
length to the UV ow cell and insert the tubing
into a waste container.
3. Fill a syringe with detergent solution and inject
the detergent solution through the UV ow cell
about ve times.
4. Leave the UV ow cell lled with cleaning
solution for 15 minutes.
5. Fill a syringe with DM water and rinse the ow
cell thoroughly.
Clean the Conductivity ow cell
Maintenance interval
Clean the Conductivity ow cell when the
Conductivity Monitor shows a slow response or
when the conductivity measurements are not
comparable to previous results.
4. Run for 3 to 5 minutes.
Operation from the Instrument
Display
Perform the following operations to clean the
Sample valve.
Clean the column
Cleaning
After a run is completed, clean the column if
required.
Instruction
• Disconnect the column from the ow path
• Clean the column off-line according to the
column instructions.

ÄKTA start Maintenance Cue Card, 29024043 AF 7
Cleaning solutions
• 1 M NaOH
• DM water
Note: Before cleaning the Conductivity cell,
remove the column from the ow path and re-
connect the ow path.
Instruction
1. Immerse the inlet tubing (either A or B) in 1M
NaOH.
2. Start a manual run and pump 1M NaOH at 1 ml/
min through the ow cell for 10 minutes.
3. Pause the run. Leave the Conductivity ow cell
lled with 1 M NaOH for 15 minutes.
4. Immerse the inlet tubing in DM water.
5. Resume the run and rinse the ow cell
thoroughly.
Note:
Do not leave NaOH for a very long time in ow
cell to avoid damage. Rinse the ow path with
water thoroughly.
Make sure that the NaOH is completely removed.
The conductivity reading in the Run view screen
should be less than 1 mS/cm.
Calibrate the Instrument Display
Calibrate the Instrument Display if there are any
issues with the response of the touch screen.
Instruction
1. In the ÄKTA start home screen, tap Settings
and service to access the instrument modules.
2. Tap Display to access the touch screen options.
3. In the Display screen, tap Touch screen
calibration to start the calibration.
4. In the four subsequent calibration screens, tap
on the marked circles, as requested.
Pressure sensor zero offset
The pressure is set as 0 with the Pressure sensor
exposed to atmospheric pressure only (Zero offset).
P set parameter displays the pressure in the ow
path.
Instruction
1. Disconnect the inlet tubing from the Pressure
sensor to expose the sensor to atmospheric
pressure only.
2. Access Settings and service > Pressure
sensor.
3. In the Pressure sensor screen, tap Zero offset
then tap OK if there is no back pressure in the
system.
Note: Make sure that the Pump is OFF before
disconnecting the tubing.
Calibrate the Pump
For detailed instructions, refer to ÄKTA start
Maintenance Manual.
Instruction
1. Immerse the buffer inlet tubing A in DM water.
2. Place the outlet tubing from the Wash valve in a
pre-weighed collection tube.
3. In the Settings and service screen, tap Pump to
access the Pump options, then tap Calibration.
4. Set the intended ow rate in the range 0.5 to 5
ml/min.
5. Tap Start ow to start the Pump and collect
water for at least one minute in the pre-weighed
collection tube.
6. Weigh the collection tube to determine
the volume of the water collected, then set
Collected volume value equal to this volume.
7. Tap Calibrate to carry out the calibration.
Calibrate the UV Monitor
Set the ow cell path length
If the UV ow cell was replaced, a new cell path
length value has to be set.
Instruction
1. In the Settings and service screen, tap UV to
access the UV Monitor options.
2. In the UV screen, tap Flow cell path length.
3. Set the cell path length, Cell, corresponding to
the new UV ow cell.
4. Tap Save path length to save the value.
Note: The Cell path length is provided on
the packing of the UV ow cell. If the ow cell
path length is not available, refer to Operating
Instructions for instructions to calculate the Cell
path length.
Calibrate the UV LED
Tip:
The life time of the UV LED can be increased by turning
off the UV LED when the instrument is idle. This can
be useful when the system is in a cold room. Refer to
Guidelines for protein purication at low temperature
(29170790).

ÄKTA start Maintenance Cue Card, 29024043 AF8
Note: We recommend to keep the instrument On to avoid
condensation in a cold room.
For more details on the procedure for turn on/off the UV
LED, refer to ÄKTA start Operating Instructions.
Note: Make sure that the UV ow cell is assembled tightly,
and the inlet and outlet tubes are tted tightly to the UV
Monitor. No stray light should enter the UV Monitor.
Instruction
1. Immerse the buffer inlet tubing in DM water.
2. Flush the UV ow cell with DM water using
Pump. Make sure that there are no air bubbles in
the UV ow cell.
3. In the Settings and service screen, tap UV to
access the UV Monitor options.
4. In the UV screen, tap UV LED calibration.
5. Set the Light strength value to 500.
6. Tap Calibrate. A conrmation screen opens.
Select OK to automatically search the Light
strength value to get a minimum Signal
response of 2700 mV.
7. If the Signal value is above 2700 mV, tap Save.
If Signal is below 2700 mV use the arrows to
increase the Light strength until the Signal is
above 2700 mV.
Calibrate the Conductivity Monitor
Temperature sensor calibration
Instruction
1. Place a precision thermometer in the ow path
directly after the Conductivity cell and pump DM
water through system with a ow rate of 0.5 ml/
min.
2. Access Conductivity > Advanced calibration.
3. Note the temperature and type it into the Set
actual temp eld, then tap Calibrate.
Sine generator calibration
Instruction
1. Use Manual run to empty the conductivity ow
path.
2. Disconnect the ow cell and dry it with a stream
of air.
3. Access Conductivity > Advanced calibration.
4. Tap Calibrate sine gen. The cell resistance
value should be 60 Mohms.
Conductivity ow cell calibration
Prerequisites
Calibration solution: 1.0 M NaCl or 100 mS/cm
conductivity standard solution.
Instruction
1. Fill the ow cell with conductivity standard
solution.
2. Access Conductivity > Calibration.
3. Note the current temperature of the calibration
solution in the conductivity ow cell as displayed
in the Cell temperature eld.
4. In the Calibration screen, enter the conductivity
value at current temperature in the Set
conductivity eld and then tap Calibrate.
Note:
For conductivity standard solutions, use the supplied
theoretical conductivity value at the current
temperature.
For 1.0 M NaCl calibration solution, read the conductivity
value at the current temperature from the graph
provided in the ÄKTA start Maintenance Manual.
Reference temperature setting
Instruction
1. Access Conductivity > Conguration.
2. Set the Set ref temp value to the temperature
shown in the Conductivity > Calibration
screen.
3. Tap Save.
Note:
The Cell constant value is set automatically when
calibration is performed. Do not change this value.
System performance test
System performance test is performed to make
sure the system is performing within acceptable
limits. It is recommended to run this method during
installation of the instrument or after replacement
of modules such as Pump, UV, Conductivity or
Valves. For a detailed description of the test, refer to
ÄKTA start Operating Instructions.
Required solutions
• Buffer A: DM water
• Buffer B: 1.0% Acetone, 1.0 M NaCl
• Sample: 1.0% Acetone, 1.0 M NaCl (Buffer B)

ÄKTA start Maintenance Cue Card, 29024043 AF 9
Checklist
• Make sure that all the modules Pressure
sensor, Pump, UV and Conductivity are
calibrated.
• Set the conductivity reference temperature to
20°C.
• No column should be present in the ow path.
• Immerse Buffer port A inlet in Buffer A (DM
water).
• Immerse Buffer port B inlet in Buffer B (Acetone/
NaCl).
• Immerse Sample valve inlet in sample (Acetone/
NaCl).
• Fit a 1 ml Sample loop and ll with sample
(Acetone/NaCl).
• Connect 2 m of 0.5 mm i.d. tubing to the Waste
position on the Outlet valve.
• When performing System performance without
Fraction collector, insert the outlet valve
fractionation tubing into a pre-weighed beaker.
• When performing System performance with
Fraction collector, connect the outlet valve
fractionation tubing to Fraction collector with at
least 5 pre-weighed tubes.
• Make sure that the system is prelled with DM
water.
Note: all the required observations by recording
parameters while the performance run is in
progress. Enter the observed values in the ÄKTA
start report template provided in ÄKTA start
Operating Instructions.
Operation from the Instrument
Display
Perform the following operations to run System
performance method.
Method run
Prepare system
System performance
method
EndRun
Exit
ÄKTA start
Acceptance criteria
Time
(min)
Activity Check Aproved
interval
0Pump wash Wash valve
position
Mobile
phase out
through
Waste
11 ml/min, 0%
B, ow through
Outlet valve,
Waste position
Back pressure ≤ 0.05
MPa
2 Repeat UV
Auto Zero
35 ml/min Back pressure 0.06 - 0.2
MPa
UV level ± 10 mAU
Cond. level ± 1 mS/cm
4 1 ml/min,
Sample
valve, Sample
position
Max UV level 300 - 380
mAU
Max Cond.
level
65 - 95
mS/cm
7 1 ml/min,
Sample valve,
Buffer position
10 Request switch
Injection valve
to Inject
position
Max UV level 300 - 380
mAU
Max Cond.
level
65 - 95
mS/cm
13 Request switch
Injection valve
back to Load
position
15 Start gradient,
0-100% B in
10 min, start
fractionation/
collection
19 End
fractionation 1
Weigh fraction
no 2, 3 and 4
0.8 - 1.2 g
Max diff.
between
fractions
0.1 g
20 End collection 2Weigh beaker 4.2 - 5.8 g
25 End gradient,
stay at 100% B
Gradient Straight,
no
negative
dips.

ÄKTA start Maintenance Cue Card, 29024043 AF10
A typical System performance test result le:
UNICORN start
System
System control
Performance Test and
Report
Operation from UNICORN start
Instruction
1. Select method based on the Fraction Collector
conguration: a) Performance method with
Frac: when Fraction collector is enabled; b)
Performance method without Frac: when
Fraction collector is disabled.
2. Read the method notes before starting the run.
3. Make a note of the result le location.
4. Run the performance method.
5. The test report states whether the performance
test passed or failed.
Note: Verify Pressure limits, Fractionation/
collection volumes, gradient levels and all
connections for leakage during the test, using
the Acceptance criteria below.
Acceptance criteria
Time
(min)
Activity Check Aproved
interval
11 ml/min, 0%
B, ow through
Outlet
valve, Waste
position
Back pressure ≤ 0.05 MPa
2 Repeat UV
Auto Zero
35 ml/min Back pressure 0.06 - 0.2
MPa
Replace the inlet lters
Note: An Inlet Filter Set containing inlet lters and
support nets is included in the accessories kit.
Instruction
1. Pull off the inlet lter and the support net from
the inlet lter holder.
2. Fit the new support net and inlet lter, and
press the lter in position into the inlet lter
holder.
Time
(min)
Activity Check Aproved
interval
15 Start gradient,
0-100% B in
10 min, start
fractionation/
collection
19 End
fractionation
(with Fraction
collector)
Weigh
fraction no 2,
3 and 4
0.8 - 1.2 g
Max diff.
between
fractions
0.1 g
20 End collection
(with-out
Fraction
collector)
Weigh beaker 4.2 - 5.8 g
25 End gradient,
stay at 100% B
Gradient (UV
50% B / UV
100% B)
Straight, no
negative
dips.
41 End Check all
connec- tions
for leakage
No leakage
Time
(min)
Activity Check Aproved
interval
28 50% B Gradient level 345 - 55% B
36 0% B (re-
equilibrate)
41 End Check all
connec-tions
for leakage
No
leakage
1with Fraction collector 2without Fraction collector 3UV
50% B / UV 100% B

ÄKTA start Maintenance Cue Card, 29024043 AF 11
Cleaning the Fraction collector
Maintenance interval
Clean the Fraction collector when required, for
example in case of liquid spill in the Bowl assembly.
Required solutions
• Water
• 20% ethanol
• Cleaning cloth
Instruction
1. Check that no run is in progress.
2. Switch off ÄKTA start and disconnect the Frac30
Cable.
3. Remove the collection tubes from the Bowl
assembly.
4. Gently move the Dispenser arm
counterclockwise to the end position (1).
5. Push the drive assembly and hold it at the
retracted position (2).
6. Lift and remove the Bowl assembly (3).
9. Let the Bowl assembly dry completely before
re-assembling.
10. Re-assemble the Bowl assembly on to the Base
unit:
• Orient the Bowl to match the aligning groove
and the aligning features on the bowl holder (1)
• Lower the bowl assembly on to the Base unit (2)
and push the drive assembly to allow the Bowl
assembly to get in position (3).
Replace the tubing and connectors
Maintenance interval
Replace tubing and connectors when the tubing is
clogged or bent so that the ow is obstructed, or
when leaking.
Note: Before starting to replace tubing and connectors,
clean the system ow path with DM water, then remove
the inlet tubing from water.
Instruction
1. Unscrew the connectors, and disconnect the
tubing.
2. If the tubing has labels, remove the labels and
use them with the new tubing later. Discard the
old tubing and connectors.
3. Cut the new tubing to the same length as the
original tubing. Use a tubing cutter to get a
straight cut.
4. Mount the connectors on the tubing.
5. Insert the tubing with connector into the port.
Make sure to insert the tubing all the way into
the bottom of the port.
6. Tighten the connector fully.
Note: To replace the Pump tubing (part no.
29024012), follow the instructions presented in
the ÄKTA start Maintenance Manual.
Storage of the
instrument
Short term storage
If the system is not going to be used for 1-2 days,
prepare the system for short term storage.
Storage solution: DM water
Note: Before cleaning the ow path, remove the column
and re-connect the ow path.
Instruction
1. Immerse the buffers and the sample inlet tubing
7. Wash the Bowl under a water tap. Use a mild
detergent if required, and rinse thoroughly with
water.
8. Wipe the surface with a damp cloth. Wipe off
stains using water.

ÄKTA start Maintenance Cue Card, 29024043 AF12
in DM water.
2. Run system methods (Pump wash A & B,
Washout fractionation tubing) or run
manually to ush the ow path (including all
inlets and outlets) with DM water. Pump at least
20 ml water through the system.
3. End the run and leave the system lled with DM
water during the storage period.
4. Open the pump cover after switching off the
instrument.
Long term storage
If the system is not going to be used for longer
than 3 to 4 days, prepare the system for long term
storage.
Storage solution: 20% ethanol (aqueous
solution)
Note: Before cleaning the ow path, remove the column
and re-connect the ow path.
Instruction
1. Immerse the buffer and the sample inlet tubing
in 20% ethanol.
2. Start a manual run and pump 20 ml of 20%
ethanol through the system.
3. Edit the run and set the run parameters to clean
the sample inlet tubing and the outlet tubing for
fraction collection.
4. End the run and leave the system lled with 20%
ethanol during the storage period.
5. Open the pump cover after switching off the
instrument.
Safety precautions
These user instructions do not guarantee user
safety. It is the responsibility of the user’s employer
to ensure safe operation and training. Training
courses are available and can be arranged by
contacting your local Cytiva ofce.
The information given in the Cue Cards suggests
best working practices and shall in no way take
precedence over individual responsibilities or local
regulations.
Great effort has been placed on the design and
manufacture of the various parts of the equipment
so that it will comply with all applicable safety
aspects for this type of equipment.
Getting help
ÄKTA start Instrument Display
Help
• Accessible from every screen on the Instrument
Display by tapping the question mark
located in the upper right corner.
• The Display Help text provides information about
the content of the current screen or refers to
more detailed documentation.
UNICORN start Online Help
• Dialog descriptions of instructions needed
to operate UNICORN start and analyze
chromatography run data.
• Accessible within UNICORN start by pressing F1
or using the Help menu.
• The online help covers the system control,
method editor and result evaluation functions in
UNICORN start.
The user documentation available with ÄKTA start is
listed in section Reference information.
Reference
information
Ordering information
For detailed information on replaceable accessories,
refer to ÄKTA start Maintenance Manual. For
ordering information visit cytiva.com/AKTA.
Health and Safety Declaration
Form
Please complete the checklist in the On Site Service
Health & Safety Declaration Form or the Health
& Safety Declaration Form for Product Return or
Servicing, depending on whether the instrument is
going to be serviced on site or returned for service,
respectively.
Copy the form you need from Operating
Instructions, Health and Safety Declaration Form,
or print it from the PDF le available on the User
Documentation CD.

ÄKTA start Maintenance Cue Card, 29024043 AF 13
User documentation for ÄKTA start
Documentation Book PDF Web Online
Operating Instructions - • • -
Maintenance Manual - • • -
System Cue Card • • • -
Maintenance Cue Card • • • -
UNICORN start User
Manual
- • • -
UNICORN start Online
Help
- - - •
Display Help - - - •

cytiva.com/akta
Cytiva and the Drop logo are trademarks of Global Life Sciences IP Holdco LLC or an afliate.
ÄKTA and UNICORN are trademarks of Global Life Sciences Solutions USA LLC or an afliate
doing business as Cytiva.
Decon is a trademark of Decon Laboratories Ltd.
Deconex is a trademark of Borer Chemie AG.
RBS is a trademark of Chemical Products R. Borghgraef S.A.
All other third party trademarks are property of their respective owner.
Any use of UNICORN is subject to Cytiva Standard Software License End-User License
Agreement for Life Sciences Software Products. A copy of this Standard Software End-User
License Agreement is available on request.
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All goods and services are sold subject to the terms and conditions of sale of the supplying
company operating within the Cytiva business. A copy of those terms and conditions is
available on request.
Contact your local Cytiva representative for the most current information.
For local ofce contact information, visit cytiva.com/contact.
29024043 AF 03/2021