Dionex UVD 170S User manual

UV/VIS Detector
UVD 170S/340S
Operating Instructions
Version: 3.01
Date: July 14, 2000
© Dionex Softron 2000
Doc.: UVD170SUVD340_OI_E_V301.doc

Declaration of Conformity
Product: UV/VIS Detector
Type: UVD 170S/340S
Dionex Softron GmbH herewith declares conformity of the above products with the
respective requirements of the following regulations:
• EN 50081-1 : 1992:
Electromagnetic Compatibility (EMC) - Generic emissions standard
Part 1: Residential, commercial and light industry
• EN 50082-1 : 1992:
Electromagnetic Compatibility (EMC) - Generic immunity standard
Part 1: Residential, commercial and light industry
• EN 61000-3-2 : 1998
Electromagnetic Compatibility (EMC)
Part 3 / Section 2: Limits for harmonic current emissions
This declaration is issued for the manufacturer
Dionex Softron GmbH
Dornierstrasse 4
D-82110 Germering
by the President, Dr. Peter Jochum.
January 16, 2001

Operating Instructions UVD 170S/340S
i
Contents
1Introduction........................................................................................................................ 1
1.1 Unpacking ..................................................................................................................... 1
1.2 Warranty........................................................................................................................ 1
1.3 How to Use This Manual .............................................................................................. 1
1.4 Intended Use of This Instrument ................................................................................... 2
2Installation.......................................................................................................................... 3
2.1 Positioning and Environment........................................................................................ 3
2.2 Mains Connection ......................................................................................................... 3
2.2.1 Fuse Replacement................................................................................................... 4
2.3 Fluidic Connections....................................................................................................... 5
2.4 Rear Panel Connections ................................................................................................ 5
2.5 Installation on the PC-Interface Board .......................................................................... 6
3Overview ............................................................................................................................. 7
3.1 Principle of Operation ................................................................................................... 7
4Operation............................................................................................................................ 8
4.1 Switching the Unit On/Off ............................................................................................ 8
4.2 Status LED on the Front Panel ...................................................................................... 8
4.3 Installation and Operation within CHROMELEONTM .................................................. 9
4.3.1 Communication Port...............................................................................................9
4.3.2 Required CHROMELEONTM Modules .................................................................. 9
4.3.3 Installation with the CHROMELEONTM Installation Tool..................................... 9
4.3.4 Operation within CHROMELEONTM ................................................................... 11
4.3.5 Spectra Calibration under CHROMELEONTM ..................................................... 12
5Troubleshooting ............................................................................................................... 13
6Maintenance .....................................................................................................................15
6.1 General Information .................................................................................................... 15
6.2 Lamp............................................................................................................................ 17
6.2.1 Lamp Removal...................................................................................................... 17
6.2.2 Lamp Replacement ............................................................................................... 17
6.3 Flow Cell ..................................................................................................................... 18
6.3.1 Flow Cell Versions ............................................................................................... 18
6.3.2 Flow Cell Removal............................................................................................... 21
6.3.3 Flow Cell Replacement ........................................................................................ 21
6.3.4 Flow Cell Cleaning............................................................................................... 21
7Technical Specifications .................................................................................................. 23
8Accessories and Spare Parts ...........................................................................................24
8.1 Standard Accessories, UVD 170S/340S ..................................................................... 24
8.2 Spare Parts................................................................................................................... 24

Operating Instructions UVD 170S/340S
1
1 Introduction
1.1 Unpacking
To unpack the unit, proceed as follows:
• Place the box on the floor and remove the accessories pack.
• Pull out the unit, slowly and carefully, using the two foam inserts.
• Remove the polythene packaging by placing the unit on a firm base and lifting at one side,
then the other, while drawing the packaging out from underneath it.
Please note: Retain all original packing materials in a safe place. It is the optimum
packaging for shipping the unit (e.g. for repair). Shipping of the unit in
any other packaging automatically nullifies the warranty.
• Check-off the contents of the accessory pack against the list in section "Standard
Accessories UVD 170S/340S", page 24)
1.2 Warranty
The standard warranty coverage for this unit is in accordance with the conditions of sale. The
warranty has a duration of one year from invoice date and covers materials and labour, ex-
works. Please note that wear-parts (i.e. needle, needle-seat, syringe and valve-seals) cannot be
covered by the warranty.
The warranty period for the deuterium lamp is six months from delivery date (see the label at
the lamp cable). Removing this label automatically invalidates the warranty coverage. For
lamps with timer (without the above label) the warranty period is 1000 operating hours.
The warranty does not extend to the flow cell.
The warranty coverage shall become invalid in any case identified as resulting from
inappropriate use, service or the implementation of non-specified spare parts. Similarly, the
warranty coverage shall be invalidated in the event of inappropriate shipment, packaging or
failure to remove aggressive or damaging solvent residues.
1.3 How to Use This Manual
The layout of this manual is designed to provide quick reference to the appropriate sections,
according to the operation required.
However, it is recommended that, before operating the UVD 170S/340S, the manual should
be read thoroughly and completely in order to obtain a full understanding of the instrument.

Operating Instructions UVD 170S/340S
2
At various points throughout the manual, messages of particular importance are indicated by
the following symbols whose relevance is as follows:
Please note: Indicates general information to assist optimum performance to be
obtained.
Important: Indicates that failure to take note of the accompanying information may
result in damage to the instrument.
Warning: Indicates that failure to take note of the accompanying information may
result in personal injury.
1.4 Intended Use of This Instrument
The UVD 170S/340S is a high-sensitivity UV-VIS diode array detector and is specifically
intended for use only for HPLC analysis and only under the control of the
CHROMELEONTM Data System.
Dionex shall not be liable for any damages, material or otherwise, which are caused by
inappropriate or improper use of this device.

Operating Instructions UVD 170S/340S
3
2 Installation
2.1 Positioning and Environment
The location of the instrument should conform to the following:
• stable and free of vibration
• free of large temperature variation and draughts
• away from direct sunlight
• capillary connections between the column and the flow cell should be of minimum length
to avoid peak broadening due to excessive dead volume.
Important: Do not place any objects or stack additional instruments on top of the
detector. The ventilation slits on top of the unit must remain free at all
times.
2.2 Mains Connection
All units are generally factory set for operation at 230V. In the event that the mains supply
varies from this, it is necessary to alter the setting of the voltage selector, located on the rear
of the instrument, immediately above the mains socket (→figs. 1 and 3). The alteration
procedure is as follows:
• Switch off the unit and disconnect the power cable.
• Draw out the fuse cartridge using a small screwdriver (→fig. 1),
• Using small pliers/tweezers to pull out the small voltage selection board from the power
supply module.
• Place the board (writing facing up) under the required voltage selection, as shown in
fig. 2.
• Turn the board so that the voltage selection can be seen, as shown in fig. 2.
• Adjust the plastic clip as shown in fig. 2 (without turning the board). Ensure that the clip
locates in the notch on the board.
• Now, replace the voltage selection board all the way back into the power supply module
(→fig. 2).
• Finally, replace the fuse cartridge.
Important: Upon installation of the instrument for the first time, the earth connection
and fuses should be checked.
Please note: For minimum interference effects, all components of the analysis system
should be connected to the same mains output.
Warning: Before attempting to alter the voltage selection or replace fuses, always
ensure that the power cable is disconnected.

Operating Instructions UVD 170S/340S
4
2.2.1 Fuse Replacement
• Switch off the unit and disconnect the power cable,
• draw out the fuse cartridge using a small screwdriver (→fig. 1),
• replace fuses as appropriate.
Important: Only use fuses as indicated in fig. 1 (min. voltage 250 V) or those listed
in the original parts list.
• If using US-type fuses, follow the procedure shown in fig. 1.
• Replace the fuse cartridge.
Fig. 1.: Removing the fuse cartridge from the power supply module
Fig. 2: Voltage selection

Operating Instructions UVD 170S/340S
5
2.3 Fluidic Connections
The capillary connections of the UVD 170S/340S are located on the left side of the detector
(→fig. 6). The input capillary is marked by the black color. Connect this capillary to the
column output.
Please note: In order to minimize peak-broadening effects due to dead-volume, it
should be ensured that all fluidic connections between sample entry and
detector are of minimum possible length.
Important: The max. pressure rating of the flow depends on the model (→Technical
Specifications, page 23). For the standard flow cell, the pressure rating is
100 bar (10,000kPa).
Important: The flow cell is primed with 2-propanol.
2.4 Rear Panel Connections
The UVD 170S/340S is controlled under the CHROMELEONTM data system via a specific
PC-interface board. Data transfer is via a special cable (order no. 1310.1501) which is
connected at the rear of the unit.
Important: Use only the original UVD 170S/340S connector cable and extension
(order no. 1310.1502) from Dionex.
The Remote Input provides a digital interface that can be used in CHROMELEONTM, e.g. to
synchronize the Inject signal of an Autosampler (or manual injection valve) with the analysis
start of the data system.
Fig. 3: Rear panel, UVD 170S/340S

Operating Instructions UVD 170S/340S
6
2.5 Installation on the PC-Interface Board
Fig. 4: Address selection on the M68-Interface board
For selection of the I/O-address, the board is fitted with a DIP-switch block (Dual Inline
Package) as shown in fig. 4. The board occupies 4 addresses, starting at the selected base
value which is factory set at 318H. Under normal circumstances, this value need not be
changed unless operating in combination with network and/or other interface boards, the
addresses of which should be confirmed before installation.
Warning: Ensure that the PC is off and the power cable disconnected before
attempting installation.
Important: The interface board must be protected against static charge at all times. If
difficulty is encountered fitting the board into the PC, please contact the
Dionex Service.
Please note: The board can be inserted in an 8-bit or 16-bit slot.
On 1 4 5 6 On 4 20 40 80
Off 2 3 7 8 Off 8 10 100 200
An open switch (OFF) indicates a binary "1", while a closed switch (ON) indicates a binary
"0". On the right, the values are converted into hexadecimal values. Determine the address by
adding the values. The default setting of the UVD 170S/340S interface board therefore has the
following base address:
8 + 10 + 100 + 200 = 318H (hexadecimal).

Operating Instructions UVD 170S/340S
7
3 Overview
The UVD 170S/340S is a high-performance diode-array detector for HPLC detection in the
UV/VIS wavelength range. The unit has been developed exclusively for operation under the
CHROMELEONTM chromatography data system.
The UVD 170S/340S features:
• Powerful optics, combined with extremely low-noise electronics enable highest
sensitivity.
• Simultaneous detection on 4 wavelengths, recording of 3D-data fields and the
comprehensive data analysis capabilities within the CHROMELEONTM data system
provide maximum analysis efficiency.
Please note: The model UVD 170S supports simultaneous detection on 4 wavelengths
if operation is via CHROMELEONTM (from version 4.10). Recording
3D-fields is not possible with this model.
3.1 Principle of Operation
Fig. 5: Principle of operation UVD 170S/340S

Operating Instructions UVD 170S/340S
8
4 Operation
The UVD 170S/340S detector is intended only for operation with the CHROMELEONTM data
system. Stand-alone operation is not possible. Data transfer is performed digitally, via a
special interface cable (→section 2.4).
4.1 Switching the Unit On/Off
Having ensured all cable connections to the UVD 170S/340S, the unit can be switched on via
the power switch on the rear of the unit.
Please note: The deuterium lamp is not automatically switched on. Lamp on/off is
performed by CHROMELEONTM. For optimum results, the lamp should
be switched on at least 30 minutes prior to analysis.
Please note: After the Lamp off command, allow 5 minutes before switching the
deuterium lamp on again. Otherwise, the lamp becomes too hot and could
be damaged (message "Lamp too hot..."). In the event that the PC is
switched off, or the connection cable to the UVD 170S/340S is
disconnected, the deuterium lamp is automatically switched off.
Important: Prior to connecting or disconnecting the UVD 170S/340S cable to the PC,
the unit must be switched off! Ensure that the connectors on the
UVD 170S/340S and on the PC are inserted evenly and that they are
correctly locked into place via the latching devices on both sides.
4.2 Status LED on the Front Panel
When the detector is switched on, the status LED on the front panel indicates the current
status of the deuterium lamp.
LED Status D2-Lamp
LED red Instrument is switched on.
LED green Deuterium lamp is switched on.
LED off Instrument is switched off.

Operating Instructions UVD 170S/340S
9
4.3 Installation and Operation within CHROMELEONTM
4.3.1 Communication Port
The UVD 170S/340S is controlled via a special PC-interface board (→section 2.4).
4.3.2 Required CHROMELEONTM Modules
To be able to control the detector UVD 170S/340S via CHROMELEONTM, the corresponding
CHROMELEONTM Control Option is required. For questions, please contact your Dionex
sales representative.
4.3.3 Installation with the CHROMELEONTM Installation Tool
Proceed as follows to install the detector UVD170S/340S in the CHROMELEONTM
Installation Tool:
• Start the CHROMELEONTM -Server and then the CHROMELEONTM Installation Tool
(= Server Configuration).
• Select the timebase to which you want to assign the detector UVD170S/340S.
• Via the Edit menu or the menu of the right mouse button, choose "Add Device... ".
• Choose "Dionex UVD170S" or "Dionex UVD340S" from the displayed list. Confirm by
pressing "OK".
The now displayed tab dialog boxes show the current configuration of your detector.
Generally, these settings must not be changed. However, please check whether the settings
correspond to your current installation environment. If necessary, change settings.
Tab Dialog Box "General"
Option Description
Device Name Instrument name under which the detector is listed in the
installation environment (default: UV)
Port Address I/O-Base address of the PC interface board (default: 318H)
Demo Mode Deactivate the demo mode!

Operating Instructions UVD 170S/340S
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Tab Dialog Box "Signals"
Option Description
UV_VIS_1
UV_VIS_2
UV_VIS_3
UV_VIS_4
3DFIELD
UVD 170S / UVD 340S
UVD 170S / UVD 340S
UVD 170S: from CHROMELEONTM v.4.10 / UVD 340S
UVD 170S: from CHROMELEONTM v.4.10 / UVD 340S
only UVD 340S
Important: Ensure that the check boxes of the required channels are activated. If not,
these channels will not be available in CHROMELEONTM.
Select the required signal, and press "Change" to view the following signal parameters:
Signal parameter Description
Signal Name Signal name
Unit Signal unit of the recorded raw data
Factor current amplification factor
Tab Dialog Box "Error Levels"
In this box you can view the classification of errors in certain error levels. The default settings
must not be altered.
Tab Dialog Box "Inputs"
Option Description
INPUT170
INPUT340
only UVD 170S
only UVD 340S
Important: Ensure that the check boxes corresponding to the remote inputs are
activated. If not, these inputs will not be available in CHROMELEONTM.
Finally, save the modified Server Configuration. Close the CHROMELEONTM Installation
Tool.

Operating Instructions UVD 170S/340S
11
4.3.4 Operation within CHROMELEONTM
In CHROMELEONTM, there are two principal modes of instrument operation:
• direct control via the toolbar and menu bar or via controls in the Control Panel
• control via time programs (PGM-file or command buttons)
The following signal-specific and global commands are available.
Signal-specific commands:
Signal type Parameters
UV-channel Step
Average
Wavelength
Bandwidth
Ref.Wavelength
Ref.Bandwidth
Signal type Parameters
3D-Field (only UVD
340S)
Min/Max Wavelength
Bunch Width
Step
Ref.Wavelength
Ref.Bandwidth
Global commands:
Lamp on/off
Autozero
Connect/Disconnect
For detailed information on controlling the detector via CHROMELEONTM, refer to the
CHROMELEONTM User Manual and context-sensitive online Help in CHROMELEONTM.

Operating Instructions UVD 170S/340S
12
4.3.5 Spectra Calibration under CHROMELEONTM
Under CHROMELEONTM, spectra calibration is performed automatically after each "Lamp
on" or "Connect" command ("detector calibration"). The command "CheckWavelength"
allows you to include the largest wavelength deviation of this calibration in the Audit Trail. A
calibration is possible only when certain conditions are met:
• During calibration, the baseline must be sufficiently stable. This may not be the case e.g.
when the solvent composition was modified or when there were air bubbles in the solvent.
• The solvent in the cell must not be fully absorbing in the wavelength range which shall be
calibrated. This will be the case e.g. if the cell is filled with 96% hexane / 4 % ethyl
acetate.
• Ensure for the calibration that the deuterium lamp is already warm because its spectrum
changes much during the first minutes after switching on the lamp.
If these conditions are not met, the process will be interrupted with the corresponding error
message. When the problem is solved, repeat the calibration using the command "Disconnect"
first followed by "Connect.
To calibrate the detector, the transmission spectrum of the deuterium lamp is compared with
that of the holmium oxide filter. With the resulting spectrum, the maxima are determined and
compared with the holmium oxide values stated in the literature. If, for a maximum, a
difference is detected between the measured and the known value, an interpolation is
performed between this maximum and the two adjacent maxima to correct the wavelength
allocation of the affected photodiodes. The spectra calibration can take up to 2 minutes.
During this time, data acquisition is not possible.

Operating Instructions UVD 170S/340S
13
5 Troubleshooting
Problem Probable cause Remedial action
Lamp is not switched
on
Lamp is still too hot
(CHROMELEONTM message:
"Lamp too hot...")
Allow a delay of 5 minutes,
then switch the lamp on
again.
Lamp cannot be switched on
( CHROMELEONTM message:
"Lamp On failed...")
Allow a delay of 5 minutes,
then switch the lamp on
again. If several attempts are
unsuccessful, check the lamp
and the connecting cable (see
below).
Connecting cable between
UVD 170S/340S and PC not
correctly installed
Connectors on the detector
and the PC must be inserted
evenly and locked into place
with latching devices on both
sides.
No function Detector not connected to
mains
Connect mains cable
Detector not switched on Switch on detector
Fuse blown Replace fuse
Replaced fuse blows
immediately
Call Dionex service
Connecting cable between
UVD 170S/340S and PC not
correctly installed
Connectors on the detector
and the PC must be inserted
evenly and locked into place
with latching devices on both
sides.
No signal / irregular
signal
Flow cell contaminated Clean flow cell
(→section 6.3.4)
Low transmission through
mobile phase
Select alternative
wavelength
Check solvent for
contaminants.
Incorrect spectral range Alter spectral range
Faulty injection valve Check fluidics
Fault in diode array Call Dionex Service
No signal / irregular
signal
Lamp intensity too low Change lamp
M68 board defective Check M68 board, replace
Connecting cable between
UVD 170S/340S and PC not
correctly installed
Connectors on the detector
and the PC must be inserted
evenly and locked into place
with latching devices on both
sides.

Operating Instructions UVD 170S/340S
14
Problem Probable cause Remedial action
Strong baseline drift Insufficient warm-up time Allow min. 30 min. warm-
up
Column contaminated Rinse or replace column
System not equilibrated Continue to rinse system
until equilibrated
Unstable environment Ensure constant temperature
and humidity
Solvent contaminated Change solvent
Flow cell contaminated Clean flow cell (→sec.
6.3.4)
Lamp defective Replace lamp
Solvent reservoir is placed too
low
Place reservoir on the same
level as flow cell
High noise level Solvent contaminated Replace solvent
Lamp aged Replace lamp
Gas bubbles in system Prime system
Pressure pulsation from pump Check and prime pump
Solvent reservoir is placed on
the floor
Place reservoir on the same
level as flow cell
Wrong wavelength Select suitable wavelength
Connecting cable between
UVD 170S/340S and PC not
correctly installed
Connectors on the detector
and the PC must be inserted
evenly and locked into place
with latching devices on both
sides.
Peak broadening Capillaries too long or has
excessive internal diameter
Shorten or replace for
narrower capillaries
Column overloaded,
contaminated or aged
Rinse or replace column
Solvent aging Replace with fresh solvent
Analysis is not
reproducible
Sample is unstable Use fresh sample or alter
conditions
Irreproducible gradient Change gradient program /
check pump
Unstable environment Ensure constant temperature
and humidity
Column overloaded,
contaminated or aged
Rinse or replace column
CHROMELEONTM Error Messages
A list of CHROMELEONTM error messages is included in the CHROMELEONTM Installation
program on the tab dialog box "Error Levels". See also section 4.3.3.

Operating Instructions UVD 170S/340S
15
6 Maintenance
6.1 General Information
The UVD 170S/340S is constructed only from the highest quality components, thus keeping
maintenance requirements to a minimum. In general, the unit should be kept clean. The
painted surfaces are resistant to solvents as well as weak acid or alkaline solutions. If solvents
or other liquids should be spilled on the surface, these should be cleaned off immediately,
using a lint-free cloth or tissue (avoid rubbing).
The following sections describe all maintenance work which can be carried out by the user.
Any other service or maintenance work should be carried out only by a qualified Dionex
personnel.
In the event that the UVD 170S/340S should require shipment for the purpose of service, this
should be only in the original packing. If no original packing is available, this can be acquired
from Dionex or your local Dionex representative.
Please note: The warranty coverage shall become invalid in any case identified as
resulting from inappropriate use, service or the implementation of non-
specified spare parts. Similarly, the warranty coverage shall be
invalidated in the event of inappropriate shipment, packaging or failure to
remove aggressive or damaging solvent residues.
All user serviceable parts (i.e. lamp and flow cell) are accessible via a removable panel,
located on the left side of the unit (→fig. 6). To open, press in and turn the knurled screw 90o
anti-clockwise. The panel may now be opened and removed. The lamp and flow cell are now
clearly visible.
Warning: During operation, the lamp becomes extremely hot and remains so for
some time after the unit is switched off. Therefore, to avoid possible
injury, always allow sufficient time for lamp cooling before attempting
any maintenance.

Operating Instructions UVD 170S/340S
16
Fig. 6: Side view, UVD 170S/340S (side panel open)

Operating Instructions UVD 170S/340S
17
6.2 Lamp
Important: Use only the original Dionex deuterium lamp. Using third party parts
may damage the instrument; and the warranty coverage shall become
invalid.
6.2.1 Lamp Removal
• If the UVD 170S/340S is still connected to CHROMELEONTM, first terminate
communication using the Disconnect command in CHROMELEONTM
(→CHROMELEONTM manual).
• Switch off power to detector and remove mains connection.
• Open and remove the side panel (see above). Do not bend the teflon capillary.
• Wait until lamp has cooled down!
• Disconnect the lamp by gently pressing either side of the locking plug and pulling out.
• Undo and remove the two lamp retaining screws (using a 2.5mm Allen-key) and carefully
remove the lamp. The Allen key is contained in the Accessories.
Warning: During operation, the lamp becomes extremely hot and remains so for
some time after the unit is switched off. Therefore, to avoid possible
injury, always allow sufficient time for lamp cooling before attempting
any maintenance.
6.2.2 Lamp Replacement
• Insert the new lamp, aligning the notch on its base with the alignment pin of the mounting
and replace the retaining screws. No additional alignment is required.
Important: Avoid touching the glass tube of the lamp.
• Reconnect cable.
• Replace the side panel.
• The detector may now be switched on and communication with CHROMELEONTM restored
using the Connect command.
Please note: A new lamp must be "run in" for at least 24 hours prior to the first
analysis. During this period, there may be strong baseline fluctuations
and increased noise.