DLab D2012 User manual

D2012 & D2012 plus High Speed Mini Centrifuge
DLAB Laboratory Instruments Limited
www.dlabsci.com

USER MANUAL
Before using centrifuge, please carefully read this user manual for its efficient operation and safety.
Contents
Copyright: ........................................................................................................................................................ 1
Safety Reminder...............................................................................................................................................2
1. Specifications...............................................................................................................................................4
2. Declaration of Conformity ........................................................................................................................... 4
3. Required Operational Condition ..................................................................................................................5
4. Installation....................................................................................................................................................5
4.1 Location.............................................................................................................................................. 6
4.2 Connection of the power cord and grounding.................................................................................... 6
5. Structure.......................................................................................................................................................6
6. Operation panel............................................................................................................................................ 8
7. Rotor Preparation ......................................................................................................................................... 9
7.1 Prepare the samples............................................................................................................................9
7.2 Inject the samples into tubes. .............................................................................................................9
7.3 Keep the tubes balance.......................................................................................................................9
7.4 Inspect the rotor..................................................................................................................................9
7.5 Symmetrically load centrifuge tubes in rotor.....................................................................................9
8. Operation....................................................................................................................................................10
8.1 Normal Operation............................................................................................................................. 10
8.2 RCF Operation .................................................................................................................................13
8.3 Pulse Operation................................................................................................................................ 13
9. Maintenance...............................................................................................................................................13
10. Troubleshooting........................................................................................................................................ 14
10.1 Frequent problems list....................................................................................................................14
10.2 How to open the door.....................................................................................................................15
11. Instructions of rotor and tube ...................................................................................................................16
11.1 The rotor instructions......................................................................................................................16
11.2 Tubes ..............................................................................................................................................17
12. Calculation Relative Centrifuge Force（RCF）......................................................................................19
13. Ordering information................................................................................................................................ 19
14. Warranty................................................................................................................................................... 20
14.1 Warranty of the centrifuge.............................................................................................................. 20
14.2 Warranty of the rotor......................................................................................................................20
15. After-sales Service.................................................................................................................................... 20

High Speed Mini Micro-Centrifuge
1
Copyright:
No part of this manual may be reproduced or transmitted without prior written permission of DLAB .
We can not be responsible to inform at real-time if the outline and specifications of centrifuge is subject to
change for improvement.
VERSION201412

USER MANUAL
2
Safety Reminder
Common safety precautions
Carefully read the following safety precautions for a thorough understanding.
Follow the instructions and procedures described in this manual to operate this centrifuge safely.
Carefully read all safety messages in this manual and the safety instructions on the instrument.
Safety messages are labeled as indicated below. They are in combination with signal words of
“WARNING” and “CAUTION” with the safety alert symbol to call your attention to items or
operations that could be dangerous to you or other persons using this instrument. The definitions of
signal words are as follows:
WARNING：Personal Danger
Warning notes indicate any condition or practice, which if not strictly observed, could result in personal
injury or possible death.
CAUTION：Possible damage to instrument
Caution notes indicate any condition or practice, which if not strictly observed or remedied, could result
in damage or destruction of the instrument.
NOTE：Notes indicate an area or subject of special merit, emphasizing either the product’s capability or
common errors in operation or maintenance.
Do not operate this centrifuge in any manner not described in this User manual. When in doubt or have
any troubles with this centrifuge,ASK FOR HELP.
The precautions described in this User manual are carefully developed in an attempt to cover all the
possible risks. However, it is also important that you are alert for unexpected incidents. Be carefully
operating this centrifuge.
WARNING：
This centrifuge is not explosion-proof. Never use explosive or flammable samples.
Do not install the centrifuge in or near places where inflammable gases are generated or chemicals are
stored.

High Speed Mini Micro-Centrifuge
3
Do not place dangerous material within 30cm around the centrifuge.
Make sure to prepare necessary safety measures before using samples that are toxic, radioactive or
contaminated with pathogenic micro-organisms at your own responsibility.
If the instrument, rotor and/or accessories that has been contaminated by solutions with toxic,
radioactive or pathogenic materials, clean it according to the decontamination procedure that you are
specified.
If you require services at site, please sterilize and decontaminate it in advance, and then notice the
service center involved in the details of the particular materials.
Do not handle the power cord or turn on or off the POWER switch with wet hands to void electrical
shocks.
For safety purposes, do not enter within 30cm around this centrifuge while it is in operation.
While the rotor is rotating, never forcedly release the door lock.
Unauthorized repairs, disassembly, and other services to the centrifuge except by our service center are
strictly prohibited.
CAUTION
This centrifuge must be located on one firm and level table.
Make sure the centrifuge is horizontal before running.
Make sure the angle between the door and cover is greater than 70 degrees when open the door.
Be careful not put your fingers or hands between the door and cover when the door off.
Do not move or relocate this centrifuge while it is running.
If fluid spills in the rotor chamber, please promptly clean and dry with a dry cloth to avoid sample
contamination.
Ensure to remove any objects and fragments of the tubes dropped inside the rotor chamber before
running this centrifuge.
Cautions on rotors
(1) Always check for corrosion and damages on the rotor surface before using it. Do not use the rotor if an
abnormality is found.
(2) Do not set the centrifuge speed beyond the allowable minimum speed of the rotor kits (rotor or
adapters). Make sure to run it below the allowable minimum speed.
(3) Do not exceed the allowable imbalance.
(4) Use the rotor and tubes within their actual capacities.
(5) If the rotor is attached with a lid, ensure it is tightened before operation.
If any abnormal condition occurs during operation, please stop it immediately and contact our service
center. Notify the service center is a warning code if displayed.

USER MANUAL
4
Vibrations are likely to damage the centrifuge, contact our service center if abnormality observed.
1. Specifications
Maximum speed
15000rpm, increment: 100rpm
Maximum RCF
15100×g, increment: 100×g
Maximum capacity
2ml×12
Timer
30 seconds -99 minutes-HOLD, continuous operation
Driving Motor
Brushless DC motor
Safety devices
Door interlock、over-speed detector、over-temperature detector、automatic
internal diagnosis
Power requirements
Single-phase, 110V-240V, 50Hz/60Hz, 3A
Dimensions（mm）
（L）255×（W）245×（H）140
Weight
6kgs
Additional features
Speed/RCF switch、Pulse operation、Processing display、Voice reminder
2. Declaration of Conformity
D2012:
Construction in accordance with the following safety standards:
EN 61010-1
Construction in accordance with the following EMC standards:
EN 61326-1/ FCC Part 15 Subpart B/ ICES 001
Associated EU guidelines:
EMC-guidelines: 2004/108/EC
Instrument guidelines: 2006/95/EC
This ISM device complies with Canadian ICES-001.
Cet appareil ISM est conforme àla norme NMB-001 du Canada.
D2012 plus:
Construction in accordance with the following safety standards:
EN 61010-1
EN 61010-2-10
Construction in accordance with the following EMC standards:
EN 61326-1/ FCC Part 15 Subpart B/ IECS 001

High Speed Mini Micro-Centrifuge
5
Associated EU guidelines:
EMC-guidelines: 2004/108/EC
Instrument guidelines: 2006/95/EC
This ISM device complies with Canadian ICES-001.
Cet appareil ISM est conforme àla norme NMB-001 du Canada.
Changes or modifications not expressly approved by the party responsible for compliance could void the
user's authority to operate the equipment.
NOTE: This centrifuge has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against
harmful interference when the centrifuge is operated in a commercial environment. The centrifuge generates,
uses, and can radiate radio frequency energy and, if not installed and used in accordance with the user
manual, may cause harmful interference to radio communications. Operation of centrifuge in a residential
area is likely to cause harmful interference in which case the user will be required to correct the
interference.
3. Required Operational Condition
3.1 Basic operational Conditions
（1）Power: 110V-240V, 50Hz/60Hz, 3A.
（2）Ambient temperature: 2℃-40℃.
（3）Relative humidity: ≤80%.
（4）No vibration and airflow around.
（5）No electric dust, explosive and corrosive gases around.
3.2 Transport and storage condition
（1）Storage temperature: -40℃-55℃.
（2）Relative humidity: ≤93%.
4. Installation
This section describes the instructions that you should abide when install the centrifuge to ensure your
safety and the optimum performance. Before moving the centrifuge, the rotor must be removed.

USER MANUAL
6
WARNING：
Improper power supply may damage centrifuge.
Make sure the power source conforms to the required power supply before connecting.
4.1 Location
（1）Place the centrifuge on a firm, flat and level table, ensure the four feet of this centrifuge stand on the
table firmly. Avoid installing on the slippery surface or surface prone to vibration.
（2）Ideal ambient temperature is 20℃±5℃, avoid placing the centrifuge in direct sunlight if temperature
exceeds 30℃.
（3）Keep clear of the centrifuge at least 10cm on both sides and at least 30cm behind it to guarantee the
cooling efficiency.
（4）Keep away from heat or water to avoid sample temperature issues or centrifuge failures.
4.2 Connection of the power cord and grounding
WARNING：
To avoid electrical shocks, ensure your hands are dry when touching the power cord.
This centrifuge must be grounded properly.
A minimum 10A outlet providing a sufficient ground is required, and this must meet with local safety
requirements.
5. Structure

High Speed Mini Micro-Centrifuge
7
Figure 5.1 Front view of centrifuge
Figure 5.2 Rear view of centrifuge
Power inlet
Power switch
Air vents
View
Door
Door lock hook
转头
Rotor
Operation panel
操作面板
Door release hole
LCD Display
foot

USER MANUAL
8
6. Operation panel
Figure 6-1 Operation Panel
Item
Symbol
Name
Function
1
Select button
Press the button to choose the program which you want to
modify.
2
Pulse button
The speed can be accelerated and held at the speed when
pressing Pulse on.
3
Parameter button
Clockwise rotate to increase program values. Rotate
anti-clockwise to decrease parameter values.
Press the button, shift between speed and RCF display.
4
Open/ lock button
Press the button to open the door The button is not available
when the centrifuge is running.
5
Start/ Stop button
Press the button to start running. The centrifuge will brake to
stop running if pressed during centrifugation.
Figure 6-2 the main interface
Main interface is as figure 6-2. The speed is set to be 15000 rpm, running time is 12 minutes. When speed
symbol is rotating, indicating the centrifuge is running. Time display symbol display the ratio of
working to time setting. The total time setting is divided into 10 sections.
Speed area Lock status Time area
150 12 min
×100rpm

High Speed Mini Micro-Centrifuge
9
7. Rotor Preparation
7.1 Prepare the samples
7.2 Inject the samples into tubes.
CAUTION：
Do not overload samples into the centrifuge which will cause leaking.
Do not exceed the actual capacity allowed in the user manual.
7.3 Keep the tubes balance
Although the centrifuge can accept sample balancing by eye, we recommend that you keep this
centrifuge in a well-balanced condition to extend its life expectancy.
Never intentionally run the centrifuge under unbalanced condition even though the allowable
imbalance is not exceeded.
7.4 Inspect the rotor
Check the rotor for corrosion or scratches before using.
CAUTION：
Any abnormality such as corrosions or scratches are found, stop using the rotor and contact our service
center.
Only manufacturer’s rotors must be used with the unit.
7.5 Symmetrically load centrifuge tubes in rotor
CAUTION：
Make sure the rotor lid is securely fixed on the rotor, as well as the rotor and shaft are tightened.
Otherwise, the rotor may be moved off while rotating and cause damage of the centrifuge and rotor.

USER MANUAL
10
8. Operation
CAUTION：
Do not push or lean against the centrifuge while it is running.
Do not run the centrifuge when fragments or sample solutions are left in the centrifuge chamber.
Always keep the centrifugal chamber clean.
If the centrifuge makes strange noise during operation, stop it immediately and contact our service
center. Notify them of the warning code if displayed.
8.1 Normal Operation
Turn on the power switch, centrifuge will display the running interface last time after passing the
self-diagnostic checks, see figure 8-1 below:
Figure 8-1 the last running interface
Speed: 15000rpm. Running time: 12 minutes.
The door lock is released.
8.1.1 Rotor loading and removal
CAUTION
Attach the rotor to the rotor shaft. Ensure the rotor is in position and connected with the shaft,
tightening the locking nut to secure the rotor with shaft, to prevent the rotor damaging the centrifuge.
Ensure the rotor lid is firmly tightened to the rotor.
150 12 min
×100rpm

High Speed Mini Micro-Centrifuge
11
Figure 8-2 the rotor installation
Load the rotor to the shaft to ensure rotor is in position until it connected well with the shaft.
Rotate the rotor slightly by your fingers to check, if the rotor vibrates, if so attach the rotor again.
Hold on the rotor with one hand, tighten clockwise the nut with the other hand, and make sure tighten
firmly.
Put the rotor lid to the rotor, press the fastener down and make the fastener attach the locking nut
tightly.
Close the door and start running.
To release the rotor, firstly pull the fastener up to remove the lid, and then turn the locking nut
anti-clockwise.
CAUTION
Check the rotor is firmly tightened before running.
8.1.2 Set the operation parameters
Press the button to select required parameters. The parameter can be modified when the parameter is
flashing. Clockwise rotate the parameter button to increase parameter value; counter-clockwise rotate the
parameter button to decrease parameter value. Parameter button rotate faster, parameter value
increase faster. The minimum speed increment is 100 rpm, the minimum time increment is 1 second.
（1）Set the speed
Press the select button until the speed rpm is displayed.
When the speed button is selected, the speed symbol will flash the speed value.
The minimum speed value you can set 500rpm, the minimum increment is 100rpm.
Rotate program button clockwise to increase speed value, rotate the program button anti-clockwise
to decrease speed value.
You can speed-up set the speed value by rotating program button quickly.
There is a circulating function to increase/decrease the speed values. Rotate the program button
Fastener

USER MANUAL
12
clockwise to change settings from small → large → maximum → minimum. Rotate the program
button anti-clockwise to change settings from large → small → minimum → maximum.
（2）Set the time
Press button , time value flashes in the time setting mode.
Rotate the program button to set running time from 30 seconds to 99 minutes.
When time displays HD, this is a continuous running mode.
8.1.3 Start the operation
（1）Press running button to start running
Timer will operate once the speed setting value is reached, the screen displays the remaining run time.
（2）View and modify the operation programs
Pressing button , returns the display to the program interface and displays settings programs. Press
the select button to the desired program. When flashing, rotate parameter button to modify
values. Release the button after 5 seconds, and the centrifuge will return to normal operation mode and
run according to the new value.
If the set time value has been modified, the operation time is not affected and will continue.
（3）Warning display
If an error occurs during the operation, the centrifuge will brake to stop automatically, and display the
error code on the time/display area. The error code can be checked in the table 10-1, and corrective
actions can be applied accordingly.
8.1.4 End the operation
（1）The centrifuge will brake when it reaches the setting time or button is pressed.
When the rotor stops rotating, centrifuge will start beeping to alert the operation has finished.
（2）Open the door
The door can be released automatically when the operation has stopped.
With the door closed, you are able to press the button to open it.
After ending the operation, the program will store the setting parameters of this operation, and will
recall these parameters when restart the program.
（3）Open the door and take out the rotor and samples.

High Speed Mini Micro-Centrifuge
13
8.2 RCF Operation
（1）Turn on the power switch.
（2）Set a RCF (Relative Centrifugal Force) value.
Press the select button and choose speed unit ×g, the speed symbol will flash into RCF value
input status.
If no button is pressed after the speed value has flashed for 5 seconds, the input mode will be shut
down.
Rotate program button to input a RCF value, RCF increment is100×g .
（3）Set operating conditions
The other operation, please refer to the section 8.1.
8.3 Pulse Operation
This function is used to remove the residual samples adhered on the interior of the tubes.
（1）Turn on the power switch and load the rotor to the shaft, tighten the rotor lid and make sure it is in
secured position, and then close the door.
（2）The centrifuge gets into preparation mode and displays last running values. The values can be reset.
（3）Press knob and hold, the centrifuge will speed up to the setting speed. While releasing
knob during acceleration, the centrifuge will start to decelerate and stop.
9. Maintenance
CAUTION
If do not follow the recommended instructions for cleaning or disinfecting may damage the centrifuge.
（1）Centrifuge
If the centrifuge is exposed to ultraviolet rays for a long time, the color of the doors may be changed or
the label may be came off. After using, cover the centrifuge with a piece of cloth to protect it from
direct exposure.
If the centrifuge needs cleaning, clean it with a cloth or sponge moistened with a neutral detergent
Note：The button works only when the rotor stopped and the door is locked.

USER MANUAL
14
solution.
Sterilize the centrifuge by wiping with a cloth moistened with 70% ethanol solution.
（2）Rotor chamber
If the rotor chamber needs cleaning, clean with cloth or sponge moistened with a neutral detergent
solution. Sterilize the centrifuge by wiping with a cloth moistened with 70% ethanol solution.
（3）Drive shaft
We recommend regular maintenance for drive shaft. You can wipe the drive shaft with soft cloth, and
then apply a thin coat of silicon grease.
（4）Door
Clean and sterilize the door using the same method as the step (1) above.
（5）Rotor
To prevent corrosion, remove the rotor from rotor chamber. If not in use for a lone term, then detach
the rotor lid and turn upside down to dry the tube holes and keep clean.
For sample leaks in the rotor, rinse the rotor with water. Apply a thin coat of silicon grease to the rotor
when it is completely dry.
The rotor should be regular maintenance, recommend to cleaning it each 3 months to ensure tube and
rotor holes keep clean, and then apply a thin coat of silicon grease.
10. Troubleshooting
10.1 Frequent problems list
This centrifuge has a self-diagnostic function. If a problem occurs, an error/warning code will be displayed
on the time display screen and the operator can determine the malfunction with the warning code below.
Symptom
Causes
Solutions
Nothing appears on the screen when
the POWER is turned on.
·Building power circuit breaker
trips.
·the fuse was blown out.
·Remove the trouble and turn
on the POWER.
·Replace the fuse.
Abnormal vibration
·Rotor do not match with
spindle
·Samples are imbalance
·Install again the rotor
·Weighting scales, install
symmetrically
CAUTION
Do not directly pour water, neutral detergent or disinfectant solution into the rotor chamber. Otherwise
fluids may leak into the drive units and cause corrosion or deterioration to the bearings.

High Speed Mini Micro-Centrifuge
15
·Rotor lid loosed
·Tighten the rotor lid firmly
Alarm code appeared on the time
display screen
E-02
Door fault
·The door opened in running.
·The button is pressed
while the door opening.
·Close the door immediately.
·Close the door，and then start
to operate.
E-06
Set wrong speed
·The setting speed exceed the
allowable range.
·Modify the speed value.
E-10～86
·Read the service manual
·Contact with service center
Table 10-1 Frequent problems and solutions
Warning code E-1～9 are related to wrong operating. You can continue running the centrifuge after the
cause removed.
10.2 How to open the door
10.2.1 In the case of power on
（1）Turn on the POWER switch, the door lock will release automatically.
（2）The door lock will release automatically once the operation finished.
（3）It is available to release the door by pressing button once the rotor stops.
10.2.2 In the case of power outage
The door cannot be opened automatically if there is a power outage. It is available to be opened manually.
（1）Ensure if the rotor has stopped rotating.
Listen carefully to ensure no rotating sound can be heard.
（2）Insert a screw driver into the hole to open the door.
Hole is located on the top right side of the unit.
Insert a screw driver into the hole and push forward to release the door.
CAUTION
The door just can be opened while the power on and rotor stops rotating.

USER MANUAL
16
11. Instructions of rotor and tube
CAUTION：
Read the instructions thoroughly, correct use rotor.
Do not exceed the allowable maximum speed of rotor、tube and adapters etc., be care that the allowable
maximum speed of some adapters are lower than the rotor’s maximum speed.
11.1 The rotor instructions
11.1.1 Rotor structure
Figure11-1 the rotor profile
11.1.2Available rotors and adapters
Table 11.1 Rotors and adapters
Model
Rotor
type
ID
code
Tubes
Adapters
Maximum
speed（rpm）
Maximum
RCF（×g）
Allowable imbalance（*）
imbalance
Volume
imbalance（**）
D2012
A12-2
01
1.5/2.0ml
tube
15000
15100
2.0g/tube
5mm/ tube
0.2ml PCR
tube
A02P2
15000
11700
Locking nut Fastener Lid
Rotor block Spindle hole Tube

High Speed Mini Micro-Centrifuge
17
0.5ml micro
tube
A05P2
15000
12780
D2012
plus
A12-2P
02
1.5/2.0ml
tube
15000
15100
2.0g/ tube
5mm/ tube
0.2ml PCR
tube
A05P2
15000
11700
0.5ml micro
tube
A02P2
15000
12780
11.1.3 Notice
The centrifuge rotor can separate sample which density lower than 2.0g/ml. if the separated samples
density is over 2.0g/ml, please calculate allowable speed depending on the following formula.
Allow Speed (rpm)= Maximum speed×(2.0(g/ml)/Sample density (g/ml))1/2
11.1.4Autoclaving
A12-2 rotor is manufactured in high-strength aluminum alloy material and can be autoclaved: 121℃(1.0kg/cm2),
20 minutes.
A12-2P rotor is made of plastic, cannot be high-pressure sterilization and UV irradiation, only ordinary
sterilization can be used.
CAUTION:
The lid of the rotor is made of plastics, can not be high-pressure sterilization, only ordinary sterilization
can be used.
11.2 Tubes
11.2.1 Cleaning and sterilizing tubes
Table 11.2 Cleaning and sterilizing conditions for tubes
O：Applicable X：Inapplicable
Condition Material
PA
PC
PP
Cleaning
Cleaning fluids
Acidic（pH5 or lower）
X
X
X
Acidic（higher than pH5 ）
O
O
O
Alkaline（higher than pH9 ）
O
X
O
Alkaline（pH9 or lower）
O
O
O
Neutral（pH7）
O
O
O
Warm water(up to 70℃)
O
O
O
Ultrasonic cleaning
Neutral detergent（pH7）
O
O
O