Endress+Hauser Analytik Jena CyBio Well vario User manual

User Manual
CyBio Well vario

II Rev B 2022/03
CyBio Well vario
Registered trademarks:
CyBio, Analytik Jena, Germany
Title to all other trademarks or brands which are referenced in this User Manual belongs to their legal
owners.
Copyright Copyright 2022 Analytik Jena GmbH. All rights reserved. No part
of this documentation may be duplicated, photocopied, saved to
a storage system or transferred to electronic media without prior
written permission of the publisher.
Publisher
Analytik Jena GmbH
Konrad-Zuse-Straße 1
07745 Jena, Germany
Germany
Tel: [phone]
Fax:[phone]
Service support
Tel: [phone]
E-Mail: [e-mail]
Ordering code number OL3381-25-300-BLE
Document type Translation of Original instructions
Serial number Refer to product nameplate

Rev B 2022/03 III
CyBio Well vario

IV Rev B 2022/03
CyBio Well vario
EC Declaration of Conformity (reproduction of the content)
Name and address of the manufacturer:
Analytik Jena GmbH
Konrad-Zuse-Straße 1
D-07745 Jena
Herewith we declare, that the product described below
CyBio Well vario
OL3381-25-300 (linear system)
OL3381-25-305 (linear system)
OL3381-25-355 (round table)
is complying with all corresponding requirements of the Machinery Directive 2006/42/EC.
In addition the product is in conformity with the EC Directive relating to electromagnetic
compatibility 2014/30/EU. The safety objectives of the Low-Voltage Directive are taken
into account.
Harmonized Standards used:
EN ISO 12100 - Safety of Machinery - General principles for design - Risk assessment and
risk reduction
EN 61010-1 - Safety requirements for electrical equipment for measurement, control and
laboratory use
EN 61000-6-2 - EMC - Generic standards: Immunity for industrial environments
EN 61000-6-4 - EMC - Generic standards: Emission for indutrial environments
The person authorized to compile the relevant technical documentation:
Analytik Jena GmbH, Konrad-Zuse-Straße 1, D-07745 Jena

Rev B 2022/03 V
CyBio Well vario

VI Rev B 2022/03
CyBio Well vario

Rev B 2022/03 VII
CyBio Well vario

VIII Rev B 2022/03
CyBio Well vario

Rev B 2022/03 IX
CyBio Well vario
Table of Contents
1 General Information ............................................................................. 1
1.1 Notes on this User Manual ...................................................................................1
1.2 Intended Purpose ..................................................................................................2
1.3 Conforming Use ....................................................................................................2
1.4 Warranty & Liability ..............................................................................................3
1.5 Scope of Delivery ..................................................................................................4
2 Technical Specifications........................................................................ 5
3 Safety Notes ........................................................................................ 11
3.1 General ............................................................................................................... 11
3.2 Standards & Guidelines ..................................................................................... 11
3.3 NRTL Certification .............................................................................................. 11
3.4 Symbols & Signal Words Used Throughout This Manual ................................ 12
3.5 Safety labeling provided at the system ............................................................. 12
3.6 Mechanical Danger Zones ................................................................................. 15
3.7 General Safety Notes ......................................................................................... 17
3.8 Requirements for Operating Personnel ............................................................ 17
3.9 Safety Requirements for Transportation & Installation ................................... 18
3.10 Safety Notes for Operation ................................................................................ 18
3.10.1 General................................................................................................................ 18
3.10.2 Compressed air ................................................................................................... 19
3.10.3 Explosion Protection, Fire Prevention ............................................................... 19
3.10.4 Electrics ............................................................................................................... 19
3.10.5 Maintenance & Care ........................................................................................... 20
3.10.6 Handling of Dangerous Substances ................................................................... 20
3.10.7 Chemical Resistance ........................................................................................... 21
3.11 Rules of Conduct in Cases of Emergency .......................................................... 22
4 Technical Description.......................................................................... 23
4.1 System Setup ...................................................................................................... 23
4.2 Component Description ..................................................................................... 25
4.2.1 Basic Unit ............................................................................................................ 25
4.2.2 Operation control panel ..................................................................................... 26
4.2.3 Pipetting heads................................................................................................... 27
4.2.4 Pipetting head CyBio Well vario 1536/8 μl ...................................................... 29
4.2.5 Capillary Head..................................................................................................... 30
4.2.6 Tip Magazine (Pipetting Head) ......................................................................... 31
4.2.7 Capillary Magazine (Capillary Head) ................................................................. 31
4.2.8 Terminals ............................................................................................................ 32
4.2.9 Compressed Air Control Unit.............................................................................. 33
4.3 Tip Wash Station 1536 Set ............................................................................... 34
4.3.1 General................................................................................................................ 34
4.3.2 Summaries .......................................................................................................... 34
4.3.3 Safety Notes........................................................................................................ 35
4.3.4 Component Description...................................................................................... 36
4.3.5 Operation ............................................................................................................ 39
4.4 Dabbing station ................................................................................................. 40
4.4.1 Introduction ........................................................................................................ 40
4.4.2 Functionality ....................................................................................................... 42
4.4.3 Composers Scripts............................................................................................... 43
4.4.4 Important Instructions for Using the Dabbing Station...................................... 43
4.5 Function ............................................................................................................. 44

X Rev B 2022/03
CyBio Well vario
4.5.1 Function With Pipetting Head............................................................................ 44
4.5.2 Function With Capillary Head ............................................................................ 45
5 Transportation & Storage ................................................................... 47
5.1 Transport ............................................................................................................ 47
5.2 Storage ............................................................................................................... 48
6 Initial Start-Up & Routine Start-Up Procedure................................... 49
6.1 Site requirements .............................................................................................. 49
6.1.1 Installation Requirements.................................................................................. 49
6.1.2 Spatial Requirements ......................................................................................... 49
6.1.3 Power Supply ...................................................................................................... 50
6.1.4 Compressed Air Supply (Only for Capillary Head)............................................. 50
6.2 Configuration & Start-Up ................................................................................... 51
6.3 Function Tests .................................................................................................... 51
6.3.1 Precision Test ..................................................................................................... 51
6.3.2 Accuracy Test ...................................................................................................... 54
6.3.3 Leak Test............................................................................................................. 55
6.3.4 Precision Test (Capillary Head).......................................................................... 56
7 Operation ............................................................................................ 57
7.1 Switching the CyBio Well vario on .................................................................... 57
7.2 Operating the CyBio Well vario from Operation Control Panel ....................... 57
7.2.1 Overview of Menu Items .................................................................................... 57
7.2.2 Menu [Manual] .................................................................................................. 59
7.2.3 Menu [Configuration] ........................................................................................ 74
7.2.4 Menu [Head change] ......................................................................................... 78
7.2.5 Special functions................................................................................................. 88
7.3 Switching the CyBio Well vario off .................................................................... 90
7.4 Specific System Details (Pipetting Head) ......................................................... 91
7.4.1 Aspiration With Additional Stroke ..................................................................... 91
7.4.2 Aspiration without additional stroke................................................................. 92
7.4.3 Volume Cycle....................................................................................................... 93
7.4.4 Waiting Time ...................................................................................................... 93
7.5 System Precision (Pipetting Head) ................................................................... 94
8 Troubleshooting.................................................................................. 95
8.1 Error Messages .................................................................................................. 95
8.2 Errors By Compressed Air Control Unit (Capillary Head) ............................... 101
9 Maintenance & Care......................................................................... 103
9.1 Safety Notes ..................................................................................................... 103
9.2 Maintenance Work .......................................................................................... 104
9.2.1 Overview .......................................................................................................... 104
9.2.2 Lubricating Transport Rails (Linear Transporting Unit) ................................ 105
9.2.3 Cleaning the Lifter ........................................................................................... 105
9.2.4 Tip Magazine (Pipetting Head) ...................................................................... 105
9.2.5 Replacement of Capillaries.............................................................................. 105
9.2.6 Cleaning the Carriage (Linear Transporting Unit) ......................................... 107
9.2.7 Cleaning the Rotary Stage (Circular Transporting Unit) ................................ 107
9.2.8 Sealing Plate (Pipetting Head) ....................................................................... 107
9.2.9 Compressed Air Control Unit – Draining Condensate .................................... 108
10 Decommissioning............................................................................. 109

Rev B 2022/03 XI
CyBio Well vario
11 Accessories &Spare Parts ................................................................. 111
11.1 Pipetting Heads & Pipette Tips ....................................................................... 111
11.2 Capillary Magazines ......................................................................................... 112
11.3 Further Accessories .......................................................................................... 112
12 Waste Disposal................................................................................. 115
12.1 Consumables .................................................................................................... 115
12.2 Reagents .......................................................................................................... 115
12.3 System & Accessories ....................................................................................... 115
A 1 CyBio Well vario System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
A 1.1 Overview .............................................................................................. 117
A 1.2 Technical Specifications ......................................................................... 118
A 1.3 Safety Notes ......................................................................................... 119
A 1.4 Device Versions ..................................................................................... 120
A 1.5 Installation & Startup Procedures............................................................ 121
A 1.6 Operation ............................................................................................. 123
A 1.7 Maintenance & Care .............................................................................. 124
A 1.8 Troubleshooting.................................................................................... 124
A 2 Scripts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127

XII Rev B 2022/03
CyBio Well vario

Rev B 2022/03 XIII
CyBio Well vario
Table of Figures
Fig. 1: Safety labeling at pipetting head and capillary head. . . . . . . . . . . . . . . . . . . . . 14
Fig. 2: Safety labeling at compressed air control unit (only capillary head) . . . . . . . . 14
Fig. 3: Safety labeling provided at the system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Fig. 4: Danger zones at pipettor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Fig. 5: Danger points at transporting unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Fig. 6: CyBio Well vario with linear (shown with stackers)
and circular transporting unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Fig. 7: General view of CyBio Well vario
(head replacement from the front version) . . . . . . . . . . . . . . . . . . . . . . . 24
Fig. 8: Type plate / connection value label. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Fig. 9: Operation control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Fig. 10: Pipetting head with magazine adapter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Fig. 11: Pipetting head CyBio Well vario 1536/8 μl . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Fig. 12: Capillary head with magazine adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Fig. 13: CyBio TipTray. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Fig. 14: Capillaries – color code and volumes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Fig. 15: View of rearside panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Fig. 16: Connector panel (for head replacement from the front version). . . . . . . . . . 32
Fig. 17: Compressed air control unit with magnetic switch for
vacuum extraction of the capillary wash station . . . . . . . . . . . . . . . . . . . 33
Fig. 18: Wash tub . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Fig. 19: Suction tub. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Fig. 20: Warning symbols on the TWS module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Fig. 21: Danger areas on the TWS module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Fig. 22: Vacuum unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Fig. 23: TWS module with 2 peristaltic pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Fig. 24: TWS module – connections (rear side). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Fig. 25: Peristaltic pump – closed and open . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Fig. 26: Insertion of Tube into Peristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Fig. 27: Peristaltic pump on TWS module
direction of rotation and delivery of liquid . . . . . . . . . . . . . . . . . . . . . . . . 39
Fig. 28: Typical behavior of the residual liquid in normal pipette tips
when resetting the pistons to the zero position . . . . . . . . . . . . . . . . . . . 40
Fig. 29: Typical behavior of the residual liquid in the case of
small pipette tips and capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Fig. 30: Removing the residual liquid from the pipette tip. . . . . . . . . . . . . . . . . . . . . . 42
Fig. 31: Complete removal of the residual liquid from the pipette tips or
capillaries by washing the dabbing fleece . . . . . . . . . . . . . . . . . . . . . . . . 43
Fig. 32: Power on/off switch on front panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Fig. 33: Power on/off switch on rear panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Fig. 34: Display screen – main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Fig. 35: Menu structure diagram view with pipetting head . . . . . . . . . . . . . . . . . . . . . 58
Fig. 36: Display – main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Fig. 37: Menu structure diagram view with capillary head. . . . . . . . . . . . . . . . . . . . . . 59
Fig. 38: Display screen – dosing – aspiration volume . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Fig. 39: Display screen – dosing – start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Fig. 40: Display screen – dosing – dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Fig. 41: Display screen – dosing – dabbing break . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Fig. 42: Display screen – pipetting – dispensing volume . . . . . . . . . . . . . . . . . . . . . . . 61
Fig. 43: Display screen – pipetting – aspiration volume . . . . . . . . . . . . . . . . . . . . . . . . 61
Fig. 44: Display screen – pipetting – start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Fig. 45: Display screen – pipetting – aspiration volume . . . . . . . . . . . . . . . . . . . . . . . . 62
Fig. 46: Display screen – pipetting – dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Fig. 47: Display screen – pipetting – residual ejection . . . . . . . . . . . . . . . . . . . . . . . . . 62
Fig. 48: Display screen – pipetting – dabbing break . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Fig. 49: Display screen – dispensing – number of volumes . . . . . . . . . . . . . . . . . . . . . 63

XIV Rev B 2022/03
CyBio Well vario
Fig. 50: Display screen – dispensing – dispensing volume . . . . . . . . . . . . . . . . . . . . . . 63
Fig. 51: Display screen – dispensing – aspiration volume . . . . . . . . . . . . . . . . . . . . . . 63
Fig. 52: Display – dispensing – start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Fig. 53: Display screen – dispensing – aspiration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Fig. 54: Display screen – dispensing – ejection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Fig. 55: Display screen – dispensing – residual ejection. . . . . . . . . . . . . . . . . . . . . . . . 65
Fig. 56: Display screen – dispensing – dabbing break. . . . . . . . . . . . . . . . . . . . . . . . . . 65
Fig. 57: Display screen – diluting – number of volumes . . . . . . . . . . . . . . . . . . . . . . . . 65
Fig. 58: Display screen – diluting – aspiration volume . . . . . . . . . . . . . . . . . . . . . . . . . 66
Fig. 59: Display screen - diluting - start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Fig. 60: Display screen – diluting – aspiration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Fig. 61: Display screen – diluting – ejection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Fig. 62: Display screen – diluting – dabbing break . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Fig. 63: Display – tip change – tip release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Fig. 64: Display – tip change – magazine removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Fig. 65: Tip magazine change (for head replacement from the front) . . . . . . . . . . . . 68
Fig. 66: Display – tip change – magazine insertion. . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Fig. 67: Display – tip change – tightening of tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Fig. 68: Display screen – rinsing - number of rinsing cycles . . . . . . . . . . . . . . . . . . . . . 69
Fig. 69: Display screen – rinsing - volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Fig. 70: Display screen – rinsing - start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Fig. 71: Display screen – rinsing - aspiration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Fig. 72: Display screen – rinsing - dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Fig. 73: Display screen - rinsing - dabbing break . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Fig. 74: Display - dispense - tip change . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Fig. 75: Display - dispense . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Fig. 76: Display - dispense with position selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Fig. 77: Display – dispensing – start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Fig. 78: Display – tip change – tip release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Fig. 79: Display – tip change – magazine removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Fig. 80: Capillary magazine change (version: head replacement from the front) . . . 72
Fig. 81: Display – tip change – magazine insertion. . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Fig. 82: Display – tip change – tightening of tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Fig. 83: Display – configuration menu (pipetting head). . . . . . . . . . . . . . . . . . . . . . . . 74
Fig. 84: A1 well target positions for 96/384/1536 well microplate. . . . . . . . . . . . . . 75
Fig. 85: Display – XY correction values . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Fig. 86: Display – configuration menu (capillary head) . . . . . . . . . . . . . . . . . . . . . . . . 76
Fig. 87: A1 well target positions for 96/384/1536 well microplate. . . . . . . . . . . . . . 77
Fig. 88: Display – XY correction values . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Fig. 89: Display – main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Fig. 90: Display – head change – remove magazine. . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Fig. 91: Remove tip magazine or capillary magazine
(head replacement from the front version) . . . . . . . . . . . . . . . . . . . . . . . 79
Fig. 92: Display – head change – insert magazine adapter . . . . . . . . . . . . . . . . . . . . . 79
Fig. 93: Insert magazine adapter
(head replacement from the front version) . . . . . . . . . . . . . . . . . . . . . . . 79
Fig. 94: Display – head change – clamp magazine adapter . . . . . . . . . . . . . . . . . . . . . 79
Fig. 95: Display – head change – remove head please . . . . . . . . . . . . . . . . . . . . . . . . . 80
Fig. 96: Open cover (head replacement from the front version) . . . . . . . . . . . . . . . . . 80
Fig. 97: Retrieval of pipetting head (from the front head replacement version). . . . 81
Fig. 98: Insertion of pipetting head (head replacement from the front version). . . . 81
Fig. 99: Display – head change – remove magazine adapter. . . . . . . . . . . . . . . . . . . . 82
Fig. 100: Removing magazine adapter (head replacement from the front version). 82
Fig. 101: Display – head change – insert magazine . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Fig. 102: Pushing magazine in (head replacement from the front version). . . . . . . . 83
Fig. 103: Display – main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Fig. 104: Display – head change – remove magazine. . . . . . . . . . . . . . . . . . . . . . . . . . 83
Fig. 105: Removal of tip magazine (head replacement from the back version) . . . . 84
Fig. 106: Display – head change – insert magazine adapter . . . . . . . . . . . . . . . . . . . . 84

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Fig. 107: Insertion of magazine adapter
(head replacement from the back version) . . . . . . . . . . . . . . . . . . . . . . . 84
Fig. 108: Display – head change – clamp magazine adapter . . . . . . . . . . . . . . . . . . . . 84
Fig. 109: Display – head change – remove head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Fig. 110: Opening the cover at the back of the pipettor. . . . . . . . . . . . . . . . . . . . . . . . 85
Fig. 111: Removal of pipetting head (head replacement from the back version) . . . 86
Fig. 112: Insertion of pipetting head (head replacement from the back version) . . . 86
Fig. 113: Display – head change – remove magazine adapter . . . . . . . . . . . . . . . . . . 87
Fig. 114: Removal of magazine adapter
(head replacement from the back version) . . . . . . . . . . . . . . . . . . . . . . . 87
Fig. 115: Display – head change – insert magazine . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Fig. 116: Insertion of tip magazine (head replacement from the back version) . . . . 88
Fig. 117: Aspiration with additional stroke . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Fig. 118: Aspiration without additional stroke . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Fig. 119: Toolset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Fig. 120: Compressed air control unit - condensate drain outlet. . . . . . . . . . . . . . . . 108
Fig. 121: Disconnectible air supply inlet. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Fig. 122: CyBio Well vario system with two stackers . . . . . . . . . . . . . . . . . . . . . . . . . 117
Fig. 123: Warning notes and safety devices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Fig. 124: Danger zones of CyBio Well vario system. . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Fig. 125: Setup versions on base frame . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Fig. 126: Cabling diagram of a CyBio Well vario system with
2 stackers and extension module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Fig. 127: Direction of rotation and delivery of liquid
for peristaltic pump at the stacker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Fig. 128: Insertion of tube into peristaltic pump. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Fig. 129: CyBio Well vario connection to a 9- or a 25-pole RS232-PC jack . . . . . . . 123
Fig. 130: Pin assignments of 9-pole D-Sub jack (CyBio Well vario) . . . . . . . . . . . . . 124
Fig. 131: Pin assignments of 9-pole D-Sub jack (stacker) . . . . . . . . . . . . . . . . . . . . . 124
Fig. 132: Example 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Fig. 133: Example 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
Fig. 134: Example 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129

XVI Rev B 2022/03
CyBio Well vario

Rev B 2022/03 1
CyBio Well vario General Information
1 General Information
1.1 Notes on this User Manual
This User Manual contains information regarding CyBio Well vario setup and function. It
also provides qualified operator personnel with knowledge as required for the safe han-
dling of the system, its components and related control and evaluation software module
CyBio Composer.
Furthermore, this User Manual includes advice on system maintenance and care, providing
references to sources of potential faults or malfunction and proposing action for removal of
faults.
This User Manual must be readily available to operating and maintenance personnel at all
times!
Conventions
Work instructions involving a timed sequence are numbered, merged to action units and
specify related results.
Lists which are not in chronological order are shown as itemized lists, sub-listings as bullet
points.
Safety notes are marked with a pictogram and the appropriate signal word (refer to sec-
tion "Safety Notes" for the meaning of pictograms and signal words used herein). They
specify the type, source and consequences of a hazard, giving advice for accident preven-
tion. A safety note always precedes instructions on required action.
Software components for control and evaluation are highlighted as follows:
Control buttons, menus, description fields, checker boxes and option fields are shown in
bold lettering enclosed in bracket (for example, [OK] button, [Devices]) menu.
Menu and selection sequences are divided by arrows
(e.g. menu [File] [Open]).
References to windows are shown in italics (e. g. window Measurement).
Field, frame and tab names are shown in bold lettering (for example, Software tab,
Position frame).
Cross references (internal) are marked with an arrow and shown in italics.
TIP
All information represents the state of knowledge at the date of printing. The manufac-
turer reserves the right to make changes if deemed necessary in the interest of technical
progress.

2 Rev B 2022/03
General Information CyBio Well vario
1.2 Intended Purpose
The CyBio Well vario provides a simultaneous multi-pipetting tool for automated process-
ing of microplates with 96, 384 or 1536 wells according to the SBS standard. Its basic
functions are: dosing, pipetting, dispensing, diluting, rinsing, tip change and pipetting
head replacement, as well as transportation of microplates. In addition, it allows non-con-
tact pipetting to be performed when the capillary head is installed. Optional extra func-
tions are, for example, the stockpiling of microplates (when working with stackers) and a
reservoir filling station. Depending on the product design version, a head may be replaced
from the front or from the back.
The CyBio Well vario pipettor is intended for laboratories doing research and development
in the fields of pharmacy, biochemistry, clinical chemistry, combinatorial chemistry, molec-
ular biology, molecular genetics and cell biology, as well as any laboratory where pipetting
operations have to be carried out in a highly parallel, rapid and precise manner.
Users are required to operate this system in no way or mode other than described or speci-
fied in this User Manual. The system's range of applications is limited by the functional
scope of available software and firmware and by the scope of delivery.
1.3 Conforming Use
The term conforming use of the CyBio Well vario presumes that:
the system is operated by qualified and trained research and laboratory personnel
all operating requirements quoted in this User Manual and described procedural
sequences and related safety notes are duly observed
all specifications in this Manual regarding system start-up, operation, preventive main-
tenance and care are met
applicable safety standards or rules are always fulfilled.
Any type of mode of application other than specified herein will be regarded as non-con-
forming use! Only the operator is liable for any damages that result from this.
The term non-conforming use will include, but is not limited to:
operating the system in medical laboratories
working with explosive substances
working in potentially explosive atmospheres
This includes compliance with all safety requirements for the protection of people and
material goods during work with radioactive, infectious, poisonous, corrosive, combustible
and other hazardous substances. The user is under obligation to fulfil all requirements on
laboratory equipment and the conduct of personnel handling substances of this nature and
on the practices in place for cleanness, sterilization, environmental protection and waste
disposal.
Users are advised to establish their own work practice and issue related instructions before
operating the system with involvement of dangerous substances Accordingly, this User
Manual contains no safety notes to protect from personal injury or material damage
TIP
The manufacturer will refuse any claims for warranty and liability for material damage
and personal injury if the CyBio Well vario is shown to have been subject to non-confor-
ming use!
TIP
CyBio Well vario system operation with involvement of dangerous substances will be
the sole responsibility of the user!

Rev B 2022/03 3
CyBio Well vario General Information
caused by substances being examined.
Process control must rely on included manufacturer software. Alterations in or damage to
the software may give rise to faults in process and damage the system or its components.
Software protection is the sole responsibility of the user.
1.4 Warranty & Liability
The period of warranty and scope of liabilities will be as stipulated under binding law and
provided for in the General Terms of Business of the manufacturer.
Warranty will be limited to repair services or replacement of damaged parts. It will exclude
consequential damage of any kind. Damage to wearing parts is not included in the war-
ranty.
Any deviation from conforming use as defined in this User Manual (operating require-
ments, process sequences) will result in restricted acceptance of warranty or liability claims
in the event of damage.
In the event of personal injury or material damage, claims for warranty or liability will be
refused, unless the CyBio Well vario can be shown to have been operated in accordance
with section →1.3 (→ „Conforming Use”).
TIP
This loss-of-warranty clause shall apply to potential periods of interruption in business
and to any system component that had not been directly affected by authorized warran-
ty work.

4 Rev B 2022/03
General Information CyBio Well vario
1.5 Scope of Delivery
CyBio Well vario system delivery includes:
CyBio Well vario
Transporting unit (linear type or circular type)
Pipetting head or capillary head
Compressed air control unit (only for working with capillary head)
CyBio Composer software
Toolset
User manual
Optional accessories