epredia Cool-Cut Manual

Company Information
© Copyright 2022. Epredia. All rights reserved.
Epredia makes every attempt to ensure that the information
contained in this supporting document is correct and clearly stated
but does not accept responsibility for any errors or omissions. The
development of Epredia products and services is an ongoing
process. Please ensure that any published information you use as
a reference is up to date and relates to the condition of the
product. If necessary, check with your local Epredia
representative.
This document may not, in whole or in part, be copied,
photocopied, reproduced, translated, or converted to any
electronic or other form without prior written consent of Epredia.
All information contained in this manual is proprietary and
confidential, and the exclusive property of Epredia and is protected
by copyright.
These instruments conform to the essential
requirements of:
•
In Vitro Diagnostic Regulation (IVDR) EU
2017/746
Symbols
The following symbols and conventions may be used
throughout this document and on the instrument:
This symbol is used on the instrument, or in a
document, to indicate that instructions must
be followed for safe and correct operation. If
this symbol appears on the instrument,
always refer to the operator guide.
This symbol is used on the instrument, or in a
document, to indicate that irritants or
potentially harmful chemicals are present.
Refer to the Material Safety Data Sheets for
the products, and always use Good
Laboratory Practice.
Contact address
Separate taking back of electrical and
electronic instruments in the countries of the
European Union: This is to be applied in the
countries of the European Union and other
European countries with a separate collecting
system within the waste management. This
product, being an electro and/or electronic
instrument, must be treated separately within
the waste management process (WEEE).
Shandon Diagnostics Limited, a subsidiary of Epredia
Tudor Road, Manor Park, Runcorn
Cheshire, WA7 1TA, UK
Tel: [phone]
Fax: [phone]
Web: www. epredia.com
This symbol is used on the instrument, or in a
document, to indicate that there are potential
biological risks associated with the instrument
and / or instrument use. Always use Good
Laboratory Practice.
USA Distributor
Richard-Allan Scientific LLC, a subsidiary of Epredia
4481 Campus Drive
Kalamazoo, MI 49008, USA
Tel: [phone]
Fax: [phone]
Web: www. epredia.com
Manufacturer
Cold surface, if necessary, use gloves
Cutting hazard, sharp edges, watch your
fingers
Consult Instructions for Use
A warning is given in the documentation if there is a
potential risk of injury, equipment failure or poor tissue
sample processing outcome.
Note
Notes give additional information about a job or
instruction, but do not form part of the instruction.

Cool Cut Operator Guide Issue 18
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Contents
Company Information 3
Symbols .........................................................3
Contact address .............................................3
USA Distributor ...............................................3
Contents 4
Safety Information 5
Introduction ....................................................5
General Safety ................................................5
Chemical Safety..............................................6
Environment ...................................................6
Warranty Statement ........................................6
Chapter 1 – Introduction 8
Chapter 2 – Operating Instructions
11
Initial turn-On ................................................11
Installing the Cool-Cut on the Rotary Microtomes
HM 310, HM 315, HM 325 and HM 340 E .....11
Installing the Cool-Cut on the Rotary Microtome
HM 335 E .....................................................12
Installing the Cool-Cut on the Rotary Microtomes
HM 355, HM 355 S and HM 360 ...................14
Installing the Cool-Cut on the Rotary Microtomes
HM 350 and/or HM 350 SV...........................15
Sectioning of Specimens...............................16
Standard Equipment .....................................18
Chapter 3 – Theory of Operation
19
Cooling of the Specimen Clamp.....................19
Chapter 4 – Working with the Cool-
Cut 20
How to avoid malfunctions ............................20
How to Proceed in Error Cases......................20
Chapter 5 – Cleaning and Care 21
Cleaning ...................................................... 21
Transportation of instrument ......................... 21
Disposal of Instrument after final
shutdown 22
Index 23
Revision Control for this Document
24

Cool Cut Operator Guide Issue 18
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Safety Information
Introduction
Epredia instruments are designed for convenient and
reliable service; however, improper use or handling
by a user may damage the instrument or cause a
hazard to health. The instrument must not be used in
a manner not specified by Epredia. Correct
maintenance procedures are essential for consistent
performance. It is recommended that users secure a
maintenance contract with our service department.
Any problems and queries should be referred to your
Epredia service department.
The following sections contain import
ant
information for the safe setup and use of
the instrument and
should be read and
understood by the user before using the
instrument.
General Safety
This instrument, as supplied, conforms to
I
EC 61010-1 and IEC 61010-2-101.
Good
Laboratory Practice must be
employed,
and
consideration must be given to the potential
for hazard when dealing with these
chemicals
.
Do not use the instrument in close proximity
to strong electromagnetic radiation, as
these may interfere with the proper
operation. The electromagnetic
environment should be evaluated prior to
operation of the device
.
Good Laboratory Practice must be used
when handling tissue samples to prevent
cross contamination and infection. The user
should complete a risk assessment to
determine any potential hazards related to
tissue handling
.
Related to the potential hazards of the
specimens to be processed personal
protection measures shall be considered:
•protection gloves
•surgical mask
•decontamination activities
•
Do not remove any panels or access
covers, unless specifically instructed to
do so. The instrument does not have any
user serviceable parts. Potentially lethal
voltages are
present inside the
instrument.
•The device may only be used wit
h the
original power supply or
an original
replacement.
Only power supply type
FW8030M/08 should be used.
•
Use only factory approved accessories
or replacement parts within the
instrument.
•Only use cleaning
reagents
recommended in the operator guide.

Cool Cut Operator Guide Issue 18
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Chemical Safety
The introduction of chemicals creates potential
hazards. Epredia has adopted the following position
with regard to the subject of volatile chemicals used
in laboratories:
•
Customers using non-
specified
chemicals in the instrument, do so at
their own risk.
•
All chemicals recommended by Epredia
have auto-
ignition temperatures
considerably above any surface
temperatures that can be reached
during a single fault failure on the
instrument.
•
The instrument contains no source of
ignition in any areas of the instrument
where chemicals are stored, or
likely to
leak into, in a single fault condition.
•
The operator is fully aware of the
contents of the specification documents
detailing the properties of the chemicals
they are using.
•
The operator has carried out any legally
required assessment of chemi
cals used
and is using Good Laboratory Practice.
Environment
This instrument complies with the European Union's
Waste Electrical and Electronic Equipment (WEEE)
Directive 2012/19/EU. It is marked with the following
symbol:
Epredia has contracts with one or more recycling /
disposal companies in each EU Member State, and
this product and packaging should be disposed of or
recycled through them. For further information
contact your Epredia service representative.
Warranty Statement
Epredia are proud of their quality, reliability and of our
after-sales service. We continuously strive to improve
our service to our customers.
Please ask your distributor or Epredia representative
about service contracts which can help maintain your
instrument in an optimal operating condition.
Warranty provisions necessarily vary to comply with
differences in national and regional legislation.
Specific details can be found in the delivery
documentation or from your dealer or representative.
Please note that your warranty may be invalidated if:
•This instrument is modified in any way, or not
used as intended by Epredia.
•Accessories and reagents which have not been
approved by Epredia are used.
•The instrument is not operated or maintained in
accordance with instructions.
•The installation of the instrument was not
conducted by a certified Epredia representative.
Any serious incident that has occurred
in relation to the device shall be
reported to the manufacturer and the
competent authority of the Member
State in which the user and/or the
patient is established

Cool Cut Operator Guide Issue 18
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Fig 1
COOL - CUT
MI CROM
MI CROM

Cool Cut Operator Guide Issue 18
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Chapter 1 – Introduction
IVDR Intended Purpose
The Epredia Cool-Cut is an in vitro diagnostic device
intended to be used alongside the rotary
microtomes. It is designed to cool samples for
taking precision sections of tissue specimens
including the sectioning of paraffin embedded
samples in medical, pharmaceutical laboratories as
necessary preparation for their diagnostic
examination. Only qualified and trained laboratory
personnel may operate the Cool-Cut.
Description cooling device Cool-Cut
The cooling device Cool-Cut includes an actively
cooled specimen clamp (fig. 2.1), an external power
supply (fig. 2.2) as well as a counterweight
consisting of pieces (fig. 2.3, 2.4 and 2.5).
Biological and human tissue specimens which are
embedded in paraffin can be cooled with the Cool-
Cut.
The Cool-Cut is available in two different versions.
One version includes a standard specimen clamp
(fig. 3.1) for paraffin blocks. The other version is
equipped with a universal specimen clamp (fig. 3.2)
for all commercially available standard embedding
cassettes.
The device can exclusively be adapted to all rotary
microtomes from Epredia.
Each current Epredia rotary microtome can easily
be equipped with a Cool-Cut

Cool Cut Operator Guide Issue 18
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Technical Data
Clamp temperature 16°C below ambient temperature
Specimen orientation: x - and y - axes universal 8°
z - axis up to 360°
Transport and Storage conditions -25°C to +55°C (at max. 60% rel. humidity)
Operating conditions: +5°C up to +35°C (at a max. rel. humidity of 60%)
altitude up to 2000 m M.S.L.
for indoor use only
Power requirements: 100-240 V / 0.6 A +/-10%/50...60 Hz
7.5V DC/3.3A
Pollution degree: 2
Protection Class Power Supply Class II Device Cool-Cut Class III
Overvoltage category: II
Acoustic pressure: 20 dB(A)
Dimensions: 180 x 60 x 95 mm (long x wide x deep)
Weight: 700 g

Cool-Cut Operator Guide Issue 18
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Fig. 2
Fig 3
COOL - CUT
MICROM
1
COOL - CUT
MICROM
2

Cool-Cut Operator Guide Issue 18
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Chapter 2 – Operating Instructions
Initial turn-On
The Cool-Cut can be adapted to all current rotary
microtomes from Epredia.
Due to different designs of the various microtome
models, the corresponding installation varies
slightly.
The installation of the Cool-Cut on the different
microtome models is described in the following
parts.
Note
Before the initial turn-on of the Cool-Cut, the type
of the respective rotary microtome must be
determined.
Installing the Cool-Cut on the
Rotary Microtomes HM 310,
HM 315, HM 325 and HM 340 E
The sequence of the below-
described steps must strictly be
adhered to. Otherwise, there is the
danger to get injured.
The Cool-Cut
is heavier than a
specimen clamp without cooling
device. For this reason, a downward
movement of the specimen head
must be anticipated after having
inserted the Cool-Cut.
•Bring the rotary microtome into its working
position. This means, the instrument must
show with its specimen holding device
towards the user.
•Lock the hand wheel brake of the respective
rotary microtome.
•Remove the knife carrier from the dovetail
guide.
•If the specimen clamp is still installed, remove
it. For this, loosen the clamping lever and
remove the clamp from the microtome.
•Remove the cover plate (fig. 4.8) which is
placed on the hand wheel (fig. 4.6) by using a
small screwdriver.
•Remove the hand wheel handle (fig. 4.5) using
the attached Allen key (size 5).
•Loosen the centre screw (fig. 4.7) of the hand
wheel using the attached Allen key (size 5)
and remove the screw.
•One part of the counterweight, the carrier
plate (fig. 4.1), is fastened together with the
hand wheel on its centre screw (fig. 4.4) via
the extended countersunk screw M8-55.
Note
The carrier plate always has four holes for
fastening it on the hand wheels of each current
Epredia rotary microtomes.
Fig 4
Now insert the hand wheel handle together with
the respective distance ring (fig. 4.2) into the
intended hole (fig. 4.3). Tighten them again on the
hand wheel via the remove screw using the
attached Allen key (size 5).
•Cover the centre screw of the hand wheel via
the self-adhesive replacement cover plate (fig.
4.8).
•Place the hand wheel counterweight (fig. 4.9)
against the carrier plate and tighten the
screws (fig. 4.10).
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The hand wheel counterweight
bears the expression "Cool-Cut".
The compensating plate (fig. 4.11)
which is similar in design has no
inscription. Its function is described
in part "2-3 Changing to
conventional specimen clamps".
•Now fit the Cool-Cut to the orienting adapter
on the microtome using the clamping lever on
the microtome.
Note
If needed, readjust the Cool-Cut on the adapter.
For this, please see part "Readjusting of
specimen clamps" in the instruction manuals of
the respective rotary microtomes
Before turning on the instrument for
the first time, please check if the
power requirements indicated on
the type p
late correspond to the
power supply voltage being used.
•Connect the power supply unit (fig. 2.2) of the
Cool-Cut with the socket.
Note
Two adapters for plugs are attached to the
power supply unit (power requirements from 100
up to 240 V, see fig. 7).
•Place the cable of the power supply beside
the microtome.
•Insert the plug (7,5 V) of the power supply unit
(fig 2.6) into the socket of the Cool-Cuts (fig.
2.7).
•The specimen clamp of the Cool-Cut is now
being cooled down.
•During operation a fan is activated, and a
diode lights up behind the clamping handle.
Note
During operation, the cooling plate heats up. On
its rear side, a fan is placed for heat elimination.
This fan must move freely and must not be
touched or stopped.
•Fix the knife carrier on the dovetail guide.
Installing the Cool-Cut on the
Rotary Microtome HM 335 E
The sequence of the below-
described steps must strictly be
adhered to. Otherwise, there is the
danger to get injured.
The Cool-Cut is heavier than a
specimen clamp without cooling
device. For this reason, a
downward movement of the
specimen head must be
anticipated after having inserted
the Cool-Cut.
•Bring the rotary microtome into its working
position. This means, the instrument must
show with its specimen holding device
towards the user.
•Lock the hand wheel brake of the respective
rotary microtome.
•Remove the knife carrier from the dovetail
guide.
•If the specimen clamp is still installed, remove
it. For this, loosen the clamping lever and
remove the clamp from the microtome.
•Remove the cover plate (fig. 5.8) which is
placed on the hand wheel (fig. 5.6) by using a
small screwdriver.
•Remove the hand wheel handle (fig. 5.5) using
the attached Allen key (size 5).
•Loosen the three decentral screws (fig. 5.7) of
the hand wheel by using the attached Allen
key (size 4) and remove the screws.
•One part of the counterweight, the carrier
plate (fig. 5.1), is fastened together with the
hand wheel on its centre screw (fig. 5.4) via
the three extended screws (fig. 5.7) using the
attached Allen key (size 4).
Note
The carrier plate always has four holes for
fastening it on the hand wheels of each current
Epredia rotary microtomes.

Cool-Cut Operator Guide Issue 18
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Fig 5
Now insert the hand wheel handle together with
the respective distance ring (fig. 5.2) into the
intended hole (fig. 5.3). Tighten them again on the
hand wheel via the remove screw using the
attached Allen key (size 5).
•Cover the centre screw of the hand wheel via
the self-adhesive replacement cover plate (fig.
5.8).
•Place the hand wheel counterweight (fig. 5.9)
against the carrier plate and tighten the
screws (fig. 5.10).
The hand wheel counterweight
bears the expression "Cool-Cut".
The compensating plate (fig. 5.11)
which is similar in design has no
inscription. Its function is described
in part "2-
3 Changing to
conventional specimen clamps".
•Now fit the Cool-Cut to the orienting adapter
on the microtome using the clamping lever on
the microtome.
Note
If needed, readjust the Cool-Cut on the adapter.
For this, please see part "Readjusting of
specimen clamps" in the instruction manuals of
the respective rotary microtomes.
Before turning on the instrument for
the first time, please check if the
power requirements indicated on
the type
plate correspond to the
power supply voltage being used.
•Connect the power supply cord (fig. 2.2) of
the Cool-Cut with the socket.
Note
Two adapters for plugs are attached to the
power supply unit (power requirements from 100
up to 240 V, see fig. 7).
•Place the cable of the power supply beside
the microtome.
•Insert the plug (7,5 V) of the power supply unit
(fig 2.6) into the socket of the Cool-Cuts (fig.
2.7).
•The specimen clamp of the Cool-Cut is now
being cooled down.
•During operation a fan is activated, and a
diode lights up behind the clamping handle.
Note
During operation, the cooling plate heats up. On
its rear side, a fan is placed for heat elimination.
This fan must move freely and must not be
touched or stopped.
•Fix the knife carrier on the dovetail guide.
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Cool-Cut Operator Guide Issue 18
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Installing the Cool-Cut on the
Rotary Microtomes HM 355,
HM 355 S and HM 360
The sequence of the below-
described
steps must strictly be
adhered to. Otherwise, there is the
danger to get injured.
The Cool-Cut
is heavier than a
specimen clamp without cooling
device. For this reason, a downward
movement of the specimen head
must be anticipated after having
inserted the Cool-Cut.
•Bring the rotary microtome into its working
position. This means, the instrument must
show with its specimen holding device
towards the user.
•Lock the hand wheel brake of the respective
rotary microtome.
Note
Rotary microtomes with motorized cutting drive
must be turned on for this, as they are equipped
with an electro-magnetic brake. For this, please
see part "Initial turn-on" in the instruction manual
of the respective rotary microtomes.
•Remove the knife carrier from the dovetail
guide.
•If the specimen clamp is still installed, remove
it. For this, loosen the clamping lever and
remove the clamp from the microtome.
•Remove the cover plate (fig. 6.8) which is
placed on the hand wheel (fig. 6.6) by using a
small screwdriver.
•Loosen the three decentral screws (fig. 6.7) of
the hand wheel by using the attached Allen
key (size 4) and remove the screws.
•One part of the counterweight, the carrier
plate (fig. 6.1), is fastened together with the
hand wheel on its centre screw (fig. 6.4) via
the three extended screws (fig. 6.7) using the
attached Allen key (size 4). The hole of the
carrier plate (fig. 6.3) is pushed over the hand
wheel handle (fig. 6.5)
Fig 6
Note
The carrier plate always has four holes for
fastening it on the hand wheels of each current
Epredia rotary microtomes.
•Cover the centre screw of the hand wheel via
the self-adhesive replacement cover plate
(fig.6.8).
•Place the hand wheel counterweight (fig. 6.9)
against the carrier plate and tighten the
screws (fig. 6.10).
The hand wheel counterweight
bears the expression "Cool-Cut".
The compensating plate (fig. 6.11)
which is similar in design has no
inscription. Its function is described
in part "2-
3 Changing to
conventional specimen clamps".
•Now fit the Cool-Cut to the orienting adapter
on the microtome using the clamping lever on
the microtome.
Note
If needed, readjust the Cool-Cut on the adapter.
For this, please see part "Readjusting of
specimen clamps" in the instruction manuals of
the respective rotary microtomes.
Before turning on the instrument for
the first time, please check if the
power requirements indicated on
the type plate correspond to the
power supply voltage being used.
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Cool-Cut Operator Guide Issue 18
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•Connect the power supply unit (fig. 2.2) of the
Cool-Cut with the socket.
Note
Two adapters for plugs are attached to the
power supply unit (power requirements from 100
up to 240 V, see fig. 7).
•Place the cable of the power supply beside
the microtome.
•Insert the plug (7,5 V) of the power supply unit
(fig 2.6) into the socket of the Cool-Cuts (fig.
2.7).
•The specimen clamp of the Cool-Cut is now
being cooled down.
•During operation a fan is activated, and a
diode lights up behind the clamping handle.
Note
During operation, the cooling plate heats up. On
its rear side, a fan is placed for heat elimination.
This fan must move freely and must not be
touched or stopped.
•Fix the knife carrier on the dovetail guide.
Installing the Cool-Cut on the
Rotary Microtomes HM 350
and/or HM 350 SV
The sequence of the below-
described steps must strictly be
adhered to. Otherwise, there is the
danger to get injured.
The Cool-Cut
is heavier than a
specimen clamp without cooling
device. For this reason, the standard
equipment includes a (hand wheel)
counterweight. However, the HM
350 S/SV is not equipped with a
hand wheel and does not need this
counterweight.
•Bring the rotary microtome into its working
position. This means, the instrument must
show with its specimen holding device
towards the user.
•Lock the hand wheel brake of the respective
rotary microtome.
Note
Rotary microtomes with motorized cutting drive
must be turned on for this, as they are equipped
with an electro-magnetic brake. For this, please
see part "Initial turn-on" in the instruction manual
of the respective rotary microtomes.
•Remove the knife carrier from the dovetail
guide.
•If the specimen clamp is still installed, remove
it. For this, loosen the clamping lever and
remove the clamp from the microtome.
•Now fit the Cool-Cut to the orienting adapter
on the microtome using the clamping lever on
the microtome.
Note
If needed, readjust the Cool-Cut on the adapter.
For this, please see part "Readjusting of
specimen clamps" in the instruction manuals of
the respective rotary microtomes.
Before turning on the instrument for
the first time, please check if the
power requirements indicated on
the type plate co
rrespond to the
power supply voltage being used.
•Connect the power supply unit (fig. 2.2) of the
Cool-Cut with the socket.
Note
Two adapters for plugs are attached to the
power supply unit (power requirements from 100
up to 240 V, see fig. 7).
•Place the cable of the power supply beside
the microtome.
•Insert the plug (7,5 V) of the power supply unit
(fig 2.6) into the socket of the Cool-Cuts (fig.
2.7).
•The specimen clamp of the Cool-Cut is now
being cooled down.
•During operation a fan is activated, and a
diode lights up behind the clamping handle.

Cool-Cut Operator Guide Issue 18
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Note
During operation, the cooling plate heats up. On
its rear side, a fan is placed for heat elimination.
This fan must move freely and must not be
touched or stopped.
•Fix the knife carrier on the dovetail guide.
Fig 7
Sectioning of Specimens
Especially at high ambient temperatures, the
lower specimen temperature generated by the
Cool-Cut has very positive effects on the
sectioning results.
The active cooling of a paraffin specimen
clamped in the specimen clamp allows thin
sections of excellent quality without re-cooling
the specimen.
Best sectioning results are achieved at constant
section thicknesses.
Note
We recommend a pre-cooling of specimens on a
cooling plate down to at least -2°C.
Sectioning with the Cool-Cut in Routine
and Research
•Compared with the ambient temperature, the
metal surface of the specimen clamp shows
a temperature which is approx. 16 Kelvin less.
•Under these conditions, the surface
temperature of a cassette or a paraffin block
clamped into a Cool-Cut, starting with a
surface temperature of -2 to -3°C, will warm
up to 10°C only after approx. 5 min.
Sectioning Serial Sections
•Depending on the ambient temperature,
specimen clamp as well as the size of the
specimen, the paraffin surface of the
specimen reaches a constant end-
temperature after approx. 15 min. This
temperature is between 10°C and 16°C. The
paraffin does not expand anymore.
•Sectioning at these constant temperature
guarantees an optimal section thickness
consistency for producing serial sections.

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Orienting the Cool-Cut on a Rotary
Microtome
For orienting the specimen, the Cool-Cut is
equipped with an identical adapter as a
conventional specimen clamp. Please see under
"Adapters for specimen clamping" in the
instruction manual of the respective rotary
microtome.
Changing to Conventional Specimen
Clamps
As an average, the Cool-Cut is 400 g heavier
than a conventional specimen clamp.
The counterweight increases the weight on the
hand wheel accordingly. Resulting in a smooth
run of the rotary microtome during the sectioning
process with the Cool-Cut. This additional weight
is solely compensated by the hand wheel
counterweight (fig. 8.2).
For this reason, changing the counterweight for
conventional specimens is very easy:
•The standard equipment of the Cool-Cut
includes one carrier plate (fig. 8.1) and two
hand wheel weights. The heavier hand wheel
weight (the actual counterweight) consists of
brass and is marked with the expression
"Cool-Cut" (fig. 8.2). The lighter one is made
of aluminium and does not have an
inscription. It is used as a compensating plate
(fig. 8.3).
Note
The heavier counterweight (fig. 8.2) with the
expression "Cool-Cut" must be used together
with the Cool-Cut.
•As described in part "Initial turn-on", put the
respective hand wheel counterweight against
the carrier plate and tighten it by the screws.
•The lighter compensating plate (without
inscription) is then tightened on the carrier
plate, in case conventional (un-cooled)
specimen clamps are used.
Fig 8
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Cool-Cut Operator Guide Issue 18
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Standard Equipment
The Cool-Cut is supplied with the following
accessories:
Cool-Cut complete with standard specimen
clamp (cat. no. 771120)
•2 screws
•1 distance ring
•1 carrier plate
•1 counterweight (brass)
•1 compensating plate (aluminium)
•1 cover disk
•3 fillister head screws M5x65
•1 countersunk screws M8x55
•3 spring disks
•1 serrated lock washer
•1 Allen key SW 5
•1 cable
•1 power supply unit
•1 Euro adapter
•1 US adapter
Cool-Cut complete with universal cassette clamp
(cat. no. 771110)
•2 screws
•1 distance ring
•1 carrier plate
•1 counterweight (brass)
•1 compensating plate (aluminium)
•1 cover plate
•3 fillister head screws M5x65
•1 countersunk screw M8x55
•3 spring disks
•1 serrated lock washer
•1 Allen key SW 5
•1 cable
•1 power supply unit
•1 Euro adapter
•1 US adapter

Cool-Cut Operator Guide Issue 18
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Chapter 3 – Theory of Operation
Cooling of the Specimen
Clamp
The Cool-Cut is an additional equipment for
cooling specimens embedded in paraffin. As the
consistency of the specimen and consequently
the sectioning results depend on the
temperature, a pre-cooling on a cooling plate is
recommended.
However, the specimen will warm up depending
on the ambient temperature and the site of
installation of the rotary microtome. As a
consequence, sectioning results tend to get
worse.
When working with the Cool-Cut, the paraffin
embedded specimens must also be pre-cooled
in a conventional way. In the Cool-Cut the
paraffin warms up slower. Even, after having
reached the end temperature after 10 min., good
sectioning results at a constant section thickness
can be achieved.
The function of the Cool-Cut is realized via
cooling the specimen clamps by means of a
Peltier element.
The cooling plate on the rear side of the
specimen clamp warms up during operation with
the Cool-Cut. In its upper area, a fan is place for
eliminating the heat

Cool-Cut Operator Guide Issue 18
20
Chapter 4 – Working with the Cool-
Cut
How to avoid malfunctions
Always use a cassette with a paraffin block which
has been pre-cooled on a cooling plate. Un-
cooled paraffin, due to its slight heat conductivity,
would need approx. 10 min. to achieve an
optimal temperature in the Cool-Cut.
When placing the cable of the power
supply unit, please note that the
cable is able to follow the cutting
movement of the Cool-Cut.
Otherwise, the cable might be
ripped out of the socket on the
Cool-Cut, or the socket or the cable
might be damaged.
How to Proceed in Error
Cases
Note
In case of an error, the Cool-Cut must only be
checked or repaired by trained skilled service
personnel.
Note
In case components or assemblies must be
replaced, only original spare parts must be used.