epredia HM 450 Manual

Company Information
© Copyright 2022. Epredia. All rights reserved.
Epredia makes every attempt to ensure that the information
contained in this supporting document is correct and clearly stated
but does not accept responsibility for any errors or omissions. The
development of Epredia products and services is an ongoing
process. Please ensure that any published information you use as
a reference is up to date and relates to the condition of the product.
If necessary, check with your local Epredia representative.
This document may not, in whole or in part, be copied,
photocopied, reproduced, translated, or converted to any electronic
or other form without prior written consent of Epredia. All
information contained in this manual is proprietary and confidential,
and the exclusive property of Epredia and is protected by copyright.
These instruments conform to the general safety and
performance of:
•In Vitro Diagnostics Regulation (IVDR) EU 2017/746
Symbols
The following symbols and conventions may be used
throughout this document and on the instrument:
This symbol is used on the equipment, or in a
document, to indicate that instructions must be
followed for safe and correct operation.
This symbol is also used on the instrument, or in a
document, to indicate that irritants or potentially
harmful chemicals are present. Refer to the
Material Safety Data Sheets for the products, and
always use Good Laboratory Practice.
If this symbol appears on the instrument always
refer to the operator guide.
Contact address
Shandon Diagnostics Limited, a subsidiary of Epredia
Tudor Road, Manor Park, Runcorn
Cheshire, WA7 1TA, UK
Tel: [phone]
Fax: [phone]
Web: www. epredia.com
This symbol is utilised on the instrument, or in a
document, to indicate that there are potential
biological risks associated with the instrument and
/ or with instrument use. Always use Good
Laboratory Practice.
USA Distributor
Due to moving parts, in connection also with the
microtome knife, a danger area arises, which
might lead to hand injuries in case of non-
compliance with the safety features of the
microtome and when disregarding the instruction
manual
Richard-Allan Scientific LLC, a subsidiary of Epredia
4481 Campus Drive
Kalamazoo, MI 49008, USA
Tel: [phone]
Fax: [phone]
Web: www. epredia.com
Cutting hazard, sharp edges, watch your fingers
Separate taking back of electrical and electronic
instruments in the countries of the European
Union:
This is to be applied in the countries of the
European Union and other European countries
with a separate collecting system within the waste
management. This product, being an electro
and/or electronic instrument, must be treated
separately within the waste management process
(WEEE).
Manufacturer
This symbol is used on the instrument, or in the
document, to indicate that instructions for use
must be consulted
A warning is given in the documentation if there is a
potential risk of injury, equipment failure or poor tissue
sample processing outcome.
Note
Notes give additional information about a job or instruction,
but do not form part of the instruction.

HM 450 Operator Guide Issue 02
4
Contents
Company Information 3
Symbols......................................................... 3
Contact address............................................. 3
USA Distributor .............................................. 3
Contents 4
EMC Statement.............................................. 5
IVD Intended Purpose..................................... 5
Safety Information 6
Introduction.................................................... 6
General Safety................................................ 6
Chemical Safety ............................................. 7
Environment................................................... 7
Warranty Statement........................................ 7
Care in Using Microtome Knife ........................ 8
Introduction to the HM 450 9
Description of Sliding Microtome HM 450........ 9
Theory of Operation........................................ 9
Working with the Microtome 10
Preparation and Orientation .......................... 10
Coarse Feed and Trimming Function ............. 10
Sectioning Instructions.................................. 10
Technical Data HM 450 ................................ 11
Operating Instructions 13
Setting Up the HM 450................................. 13
Initial Turn-On............................................... 13
Cutting Movement and Retraction................. 14
Locking the Sledge....................................... 14
Setting Section Thickness and Trimming
Thickness .................................................... 15
Specimen Feed............................................ 16
Operating Panel ........................................... 18
Specimen Clamping ..................................... 22
Knife Carrier ................................................. 23
Standard Equipment 26
Additional Equipment (Optional)..................... 26
Troubleshooting 28
Preparation of the Specimen......................... 28
Temperature of the Specimen ....................... 28
Tightening the Clamping Screws ................... 28
Selection of the Knife .................................... 28
Adjustment of the Knife................................. 28
Cutting Speed .............................................. 28
Trimming...................................................... 28
Appendices 29
Appendix A – Maintenance............................ 29
Appendix B - Cleaning and Care ................... 29
Appendix C - Transportation of the Instrument30
Revision Control For This
Document 33

HM 450 Operator Guide Issue 02
5
EMC Statement
This IVD equipment complies with the emissions and
immunity requirements of IEC 61326-2-6.
This equipment has been designed and tested to
CISPR 11 Class A.
It is intended for use in a laboratory environment by
a trained and qualified professional. In a domestic
environment it may cause radio interference, in
which case it may be necessary to take measures to
mitigate the interference.
IVD Intended Purpose
The Epredia sliding microtome HM 450 is an in vitro
diagnostic device, designed to take precision
sections of tissue specimens including the
sectioning of paraffin embedded samples in medical,
pharmaceutical laboratories as necessary
preparation for their examination. Only qualified and
trained laboratory personnel may operate the HM
450
The instrument may only be operated within the
scope of its intended use as described above and as
per the instructions given in this manual.
Any other use of this instrument is considered as an
improper action.