Four E's Scientific MultiEX 032 User manual

Operating Instructions
Nucleic Acid Extraction System MultiEX 032

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Contents
1. Introduction
- Intended Use
- Working Principles & Steps
2. Safety Instructions
3. Unpacking & Installation
4. Operating Instructions
5. User Interface
6. Trouble shooting
7. Error Codes
8. Maintenance & Care
9. Warranty
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Thank you for purchasing MultiEx 032 Nucleic Acid Extractor.
Users should read this manual carefully, follow the instructions and
procedures, and beware of all safety instructions when using this
instrument. Please keep the manual in an easy accessible place for
reference. In this manual, "user" refers to an operator who has been
systematically trained. Any operations beyond this manual is forbidden,
which will void your warranty.
1. Introduction
MultiEX 032 is an automated nucleic acid extraction and purification
system which can process up to 32 samples per run. The system uses
Magnetic Bead Separation technology, and has the capability of extracting
and purifying nucleic acids from a variety of biological samples, such as
whole blood, viruses, tissues, plants, animals, fungi, bacteria, cultured
cells, swabs, feces, blood cards, etc. The system comes with a user-friendly
touch screen interface allowing the user to easily set purification
parameters. The automated system integrates all the purification phases
including lysis, binding, mixing, washing, and elution, and delivers high
-efficiency, high-quality purification. The system works with any prefilled
magnetic bead-based reagent kit.
- Intended Use
Application: For extracting and purifying DNA and RNA from animal and
plant tissues, blood, body fluid etc.
Fields of Application: It is widely used in routine scientific research,
genomics, food safety, disease diagnosis, forensic identification, and
other fields.
- Working Principle and Steps
The MultiEX series extractors works on the principle of magnetic bead
adsorption.
MultiEX 032 Automated nucleic acid extraction system can process up to
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Lysis Buffer
Elution Buffer
Wash Buffer
Wash Buffer
Wash Buffer
Magnetic Beads
Lysis Buffer
Elution Buffer
Wash Buffer
Wash Buffer
Wash Buffer
Magnetic Beads
Load Liquid Sample
Load Liquid
Sample
Load Liquid
Sample
Collect and transfer
magnetic beads to
lysis buffer column
Sample lysis and binding of
nucleic acids to magnetic beads
Transfer magnetic beads to
wash buffer column
Magnetic Rods
Magnetic Rod Sleeves
Deep-well Plate
Stir up
and down
1 2 3 4 5 6 7 89 10 11 12
Stir up and down
1 2 3 4 5 6 7 89 10 11 12 1 2 3 4 5 6 7 89 10 11 12
Lysis/Nucleic Acid Binding: Lysis buffer solution promotes cell rupture and releases
nucleic acids. Magnetic beads transferred from wells 2/8 to the lysis wells binds to the
target nucleic acid molecules. The magnetic beads with the bound particles are then
moved to the wells 3/9 for washing.
32 samples per run. The automated system integrates lysis, binding,
mixing, washing, and elution steps to extract nucleic acid of high purity.
Magnetic beads, with strong affinity and adsorption force for nucleic acids,
binds to the nucleic acid released on lysis. The nucleic acids bound to the
magnetic beads can be transferred to different wells using the magnetic
rods. The magnetic rod sleeves facilitate quick and even mixing of the
buffer and the nucleic acid bound beads.
Working Principle and Steps
Sample Loading
Remove the Sealing Film
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Elution Magnetic beads recovered Returned to
initial position
1 2 3 4 5 6 7 89 10 11 12 1 2 3 4 5 6 7 89 10 11 12 1 2 3 4 5 6 7 89 10 11 12
Dry and transfer magnetic
beads to elution column
1 2 3 4 5 6 7 89 10 11 12
Stir up
and down Stir up and down
1 2 3 4 5 6 7 89 10 11 12
3rd Wash
1st Wash 2st Wash
Stir up
and down Stir up and down
1 2 3 4 5 6 7 89 10 11 12
Washing: Alcoholic wash buffers in the wash wells facilitate the removal of contaminants.
Since the magnetic sleeves were soaked in alcohol during the wash stage, they need to
“air dry” above the wells so that the alcohol can volatilize before starting the elution stage.
Stir up
and down Stir up and down
1 2 3 4 5 6 7 89 10 11 12
Elution: After ethanol is removed, the nucleic acid bound to the magnetic beads are
mixed in elution buffer, releasing the nucleic acid.
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2. Safety Instructions
Standards & Regulations
The product is manufactured in accordance with the following standards:
Safety Standards:
EN 61010-1 : 2020 / A1 : 2019, EN 61010-2-010 : 2020,
EN 61010-2-101 : 2017, UL 6101-1
EMC Standards:
EN 61326-1 : 2013, EN 61326-2-6 : 2013
Associated EU guidelines：EMC-guideline 89/336/EWG
We declare under our sole responsibility that this product corresponds
to the directives
2014/35/EU, 2014/30/EU and 2011/65/EU
The meanings of safety precaution marks are as follows：
WARNING！
“WARNING” indicates a dangerous condition that may lead to death
or serious injury.
CAUTION！
This symbol is used to indicate that non-compliance with instructions or
procedures may lead to physical injury or even death or could cause
damage to the instrument.
Important！
“Important”shows the important notes for usage, as well as prohibited
actions.
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Note
“Note” indicates the notes, procedures that should be obeyed and
supplementary information for use.
HOT SURFACE
This symbol is used to label potentially hot instrument surfaces.
MECHANICAL HAZARD
During operation, there is a risk of crushing, puncture, or injury from
moving parts.
BIOHAZARD
This symbol is used to indicate that certain precautions must be taken
when working with potentially infectious material.
For your safety and that of others, follow the guidelines provided in the
following pages concerning the use of the MultiEx 032 system.
WARNING:
Ignoring the following may lead to fire or electric shock.
• Do not use the device with voltage other than the specified voltage.
• The input power supply must be stable
• Do not use the device with a damaged power plug or a loose socket.
• If there is dust on the prongs of the power plug or on the plug socket,
remove it with a dry cloth.
• When you disconnect the plug from the outlet, be sure to hold the
power plug itself. Do not pull the power cable.
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• Do not plug or unplug the power supply with wet hands.
• For maintenance, disconnect the power plug from the outlet.
• If any liquid spills into the instrument, it may cause malfunction and
electric shock.
• Do not place any objects containing liquid on the device. Doing so may
cause a device failure, fire, or electric shock.
• In the event of spillage, please immediately turn off the power and
contact supplier representative or manufacturer.
• In the event the device overheats, emits smoke or strange smells,
immediately unplug the power cable.
CAUTION！
• Never attempt to reconfigure or disassemble the device without the
manufacturer’s permission. Doing so may cause fire or electric shock.
• In case there is any liquid residue inside the device, wipe it up a soft
paper tissue, etc.
• Repairs to the MultiEx 032 system should only be performed by
personnel specifically authorized by Manufacturer.
• Only original replacement parts should be used.
• If the equipment is used in a manner not specified by the manufacturer,
the warranty may be impaired.
MECHANICAL HAZARDS
WARNING！
• The moving parts of this system may cause personal injury during
operation. While running, the door must be closed. User shouldn’t
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extend hands into the working area while the sample run or calibration
is in progress.
HOT SURFACE
• Touching the heating pads while in operation may cause burn injury.
User shouldn’t touch the heating blocks during or immediately after
operation.
Regarding Reagent Kit：
CAUTION！
Note: The kits are generally not supplied with the Device. Select the
appropriate kit to suit your requirements.
• Reagents in the kits should be handled by observing the safety
information and precautions regarding the kit.
• Extraction should be performed in an appropriate laboratory or
workplace.
Regarding Samples:
BIOHAZARD
• Always wear appropriate gloves, and PPEs when handling any infectious
samples.
Waste Handling：
• When handling or disposing of infectious materials, follow the laboratory
guidelines or the law regarding disposal of hazardous waste.
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3. Unpacking & Installation
3.1 Unpacking
Unpack the device carefully and check for any damages which may have
arisen during transit. Please contact the manufacturer/supplier for
technical support. Note: If there is any apparent damage to the device,
please do not connect the power line
3.1.1 Contents of the Package
Item
Main Unit
Power cord
User manual
Allen wrench
Phillips screwdriver
QTY
1 PC
1 PC
1 PC
1 Set
1 PC
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① Glass panel
② 10-inch color touch screen
③ Magnetic rods
④ Magnetic rod sleeves
3.2 Device Installation and Setting Up
3.2.1 Operating conditions
This automatic nucleic acid extractor must be installed and used indoors
in environment that meets the following conditions:
3.1.2 System Structure
This automatic nucleic acid extraction system consists of main housing,
mechanical movement mechanism, magnetic rods, color touch screen,
UV sterilizer, heating and temperature control modules, and control system.
①
②
③
④
⑤
⑥
⑦
⑧
⑨ Ethernet port⑤ 96 deep-well plates
⑥ Heating pads
⑦ USB port
⑧ Power port
⑨
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①
②
③
④
①
②
③
④
• Environment temperature: 10℃～40℃
• Relative humidity: 30%～80%
• Power supply: 200-240VAC, 100-120VAC; 50/60Hz
• Good ventilation and no direct sunlight
• The installation requires a flat and stable space of no less than 65cm×
50cm (length × width), and capable of bearing a load of more than
70KG.
3.2.2 Setting Up
Before starting the device, the user must remove the fixing mechanisms
as indicated below. Powering on before removing this may damage the
device.
1）Take the machine from the package and remove the foams.
2）Unscrew 4pcs of screws at positions ①②③ with the Allen wrench in
the package and take the fixing plate away to release the moving
components.
3）Remove the screw at position ④ to release heating platform.
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3.2.3. System Specifications
1-32
2
96-well deep-well plate
Magnetic bead-based open-source reagents
50-1000μL
Room temp.~140℃
349mm*325mm*390mm
Adjustable: 8 Levels
10Hz (10 times up and down in 1 second)
10-inch color touch screen
96%
CV＜5%
20min-40min/run (depending on
reagent types and volumes）
Yes
Create, edit, and delete options available
＞500 programs
1 USB port, Ethernet port
200-240V, 100-120V; 50/60 Hz
Fan
25KG
＜50db
Sample Quantity
Plate Quantity
Plate Type
Reagent Type
Volume Capacity
Heating Temp.
Dimension
Mixing Intensities
Max. Mixing Speed
Display
Magnetic Bead Recovery Rate
Inter-well Purification Variation
Processing Time
UV light
Program management
Data Storage Capacity
Extension ports
Power Supply
Exhaust
Weight
Noise
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4. Operating Instructions
4.1 Power connection
Connect one end of the power cord to the power port on the back of the
instrument and the other end to the power socket, then turn on power.
4.2. Installation and Removal of Magnetic Rod Sleeves
Installing the sleeves: Insert the magnetic rod sleeves fully into the grooves.
Press the buckle at the end of the sleeve and ensure it is fully inserted
and in position.
Removing the Sleeves. Press the buckle at the end of the sleeve and pull
it out of the groove.
4.3 Installing the deep-well plates
Slide the platform out using the handle. Place the bottom side of the
deep well plates on to the heating pads, aligning the side of the plate
against the two positioning pins ① in the middle. Press down till the
deep-well plates is completely in position and you can hear a ‘click’ sound.
①
②
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5. User Interface
5.1 Start up
Close the door, and turn on the power switch on the back of the
instrument and wait for the start-up display.
The Home Screen has Five Main Menus which can be accessed from the
side bar:
1. Run Prog., 2. Edit Prog., 3. System Settings,
4. UV Light 5. Power Off
①
①
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5.2 Create & Edit Programs
To create or edit program, press the ‘Edit Prog.’ Icon on the Home
screen side bar menu.
‘Edit Prog.’ Menu Options
The following options can be accessed from the bottom menu bar
• Create: To create new programs
• Edit: Edit Program Parameters of a selected program
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• Copy: Copy a Selected Program
• Paste: Paste a copied program (need to rename while pasting)
• Delete: Delete a selected program
Note: In the ‘Edit Program’ Menu a list of existing programs will be
shown in chronological order. Long press any program to edit
‘Program Name’ and ‘Remarks’.
icon is to choose a program as ‘frequently used program’. The
selected program will show up as icons under the ‘Run Prog.’ menu
for easy selection.
5.2.1‘Edit Prog.’ Instructions
Creating New Programs: Programs can be created in two ways.
• Option 1 -‘Create’ option: Use this option when creating an entirely
new program, and while operating the instrument for the first time.
Once the program is created all program parameters needs to be
entered for each step.
• Option 2 -‘Copy’ + ‘Paste’ (Copying an existing program):
Saves time if you want to create a new program by editing the
program parameters of an existing program.
Option 1: ‘Create’
Create new program: Click “Create”button. Enter ‘Program
Name’and any program ‘Remarks’you want to enter. Click ‘Confirm’.
Once you confirm the program will appear on the bottom of the Program
List on the “Edit prog.” Page.
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Edit Parameter: After creating the new program, you need to set corresp
-onding parameters for each step by clicking “Edit” button.
Scroll down to the bottom of the Program List to see the newly created
program and click to select the program. Then click the “Edit” button
on the bottom menu to edit program parameters. This will take you to
the ‘Edit Parameters’ page. For detailed instructions on how to add
steps and edit program parameters, please see section 5.2.1.3 Editing
Program Parameters.
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Option 2: ‘Copy’ + ‘Paste’
Copy program & Paste Program: From the program list in the ‘Edit Prog.’
Menu, select the program you want to copy by clicking on the program.
Then click “Copy” button in the bottom menu to copy the program.
After copying the program, click the “Paste” button in the bottom
menu to create a new program by replicating the program parameters
of the old program. You will be prompted to rename the program on
clicking the “Paste” button. Enter new name and click ‘Confirm‘.
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The newly renamed program will appear just below the copied program.
Click on the program to select. Then click the “Edit”button on the bottom
menu to edit program parameters. This will take you to the ‘Edit
Parameters’page. For detailed instructions on how to add steps and
edit program parameters, please see section 5.2.1.3 Editing Program
Parameters.
5.2.1.2 Deleting Programs
Select any program you want to delete and click the ‘Delete’button in
the bottom menu. Click ‘Confirm’in pop-up to complete deletion.
5.2.1.3 Editing Programs Parameters
On the ‘Edit Prog.’ Page, scroll through the program list to select a
program you want to edit / add parameters (Newly created programs
will be on the bottom of the list, while copied programs will appear just
below the original program which was copied). Then click the “Edit”
button on the bottom menu to edit program parameters. This takes you
to the ‘Edit Parameters’ page.
The following functions can be executed in the ‘Edit Parameters’page.
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