Genevac HT-24 User manual

Genevac HT-24
Evaporation System
User Manual
Original instructions
Part number 10-1562 Issue 3-7 March 2014

Contents
Introduction...........................................2
Safety.....................................................5
Warnings and cautions ...........................5
Risk assessment.....................................5
Precautions.............................................5
Combustible solvents..............................6
Electrical earthing ...................................6
Strong acids............................................6
Low auto ignition point solvents..............6
Options..................................................7
Standard equipment................................7
Auto defrost and drain.............................7
High power lamps...................................7
Fume hood..............................................7
Sample holders.......................................8
Basic Operation ....................................9
Power up / down.....................................9
Opening / closing the door....................11
Stand-by mode......................................11
Workstation controls .............................12
Evaporator controls...............................13
LCD screen...........................................14
SampleGuard™ ....................................15
Selecting a run......................................16
Starting a run ........................................16
Run in progress.....................................17
Stopping a run.......................................17
End of run .............................................18
Advanced Operation...........................19
Setting-up a method..............................19
Run parameters....................................20
Run log..................................................28
Options menu........................................28
Controls menu.......................................29
Menu bar...............................................29
Data transfer .........................................30
Condenser...........................................31
Automatic defrost and drain..................31
Manual defrost and drain......................32
Waste solvent container........................32
Loading Samples................................34
Sample holders.....................................34
Use of correct accessories ...................34
Mass limit..............................................35
Sample holders and swings..................35
Loading the rotor...................................36
Balancing the rotor................................37
Method guide.......................................38
Pre-programmed runs...........................38
Creating new methods..........................39
Method optimisation..............................39
Lyophilisation........................................39
Acceptable solvents..............................40
Solvent mixtures ...................................41
Method examples .................................42
Maintenance........................................ 45
Recommended practice........................45
Periodic maintenance ...........................45
General cleaning...................................46
IR lamp lenses......................................46
Sample holders.....................................46
Planned maintenance and servicing.....47
Delivery and Installation..................... 48
Checking the delivery ...........................48
Installation site......................................48
User training..........................................48
Troubleshooting..................................49
Audible alerts........................................49
Visual alerts ..........................................49
Faults and errors...................................49
Clearing Errors......................................49
Condensation........................................50
TFA creep.............................................50
Bumping................................................51
Opening the door without power...........51
Circuit breakers.....................................51
Rectifying faults and errors...................52
Technical Data.....................................54
Specifications........................................54
EC Declaration of Conformity...............55
Warranty statement ..............................56
Patents..................................................56
Amendment control...............................57
Useful Information.................................58

Genevac HT-24
User Manual
210-1562 Issue 3-7 March 2014
Introduction
Genevac HT evaporation systems are designed to provide very high performance, coupled
with ease of use. They are simple to set up, easy to operate and very flexible.
The HT-24 Workstation occupies the minimum amount of laboratory space, comprising two
HT-8 or HT-12SII evaporators (or a combination of both) mounted on a workstation trolley
with the option of an integrated fume hood. The workstation also accommodates two
condensers, a high vacuum scroll pump and a waste solvent container.
Scroll pump & catch-pot
door open
Workstation front view
Two HT12-SII evaporators installed with optional fume
hood. Note: The fume hood has three sliding doors (not
shown) for easy access.
Waste solvent container
door open
The evaporators can be operated independently, one unit can operate while the other is
emptied and reloaded, or different solvents can be removed simultaneously in each
evaporator (providing waste solvents do not react within waste container).

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The two evaporators share a single scroll pump via a vacuum valve manifold which
intelligently switches the pump to each evaporator on demand. The scroll pump is a dry
vacuum pump, compact in size, air cooled and driven directly by a single-phase electric
motor.
Data Logging is available, either by transferring files via a USB storage device, or by
connecting each evaporator independently to a PC.
Rear view with fume hood rear panel
removed for clarity
Two data connections
& waste solvent pump
Three mains power supply
connections & electrical
circuit breakers
The optional fume hood fits on top of the HT-24 workstation and provides a totally enclosed
system. There are three transparent sliding doors in the front for easy access to the
evaporators and a window in each side. An exhaust spigot in the roof provides for
connection to a suitable fume extraction system and a flow meter is located in the top right
corner to monitor performance of the extraction system.

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Convenient pull-out sample shelves provide assistance when loading / unloading the
evaporators.
Fume hood flow meter
Pull out sample shelves
Automatic end of run detection software can be utilised to complete evaporation runs in the
shortest possible time, opening the possibility for two or more runs to be completed in a day
where previously overnight running may have been required.
User interaction is via an easy-to-clean touch-sensitive keypad and data is displayed on a
bright and clear LCD screen. Once set-up, operation is straight forward, simply load
samples, select the appropriate run and press start.
Evaporator screen and keypad
Evaporation progress is shown by a graphical display with plots for temperature and
pressure.
The evaporator controls the condenser and feed-back signals prevent the system initiating
an evaporation process until the condenser is ready to start. Waste solvent defrost and
drain can be set up to occur automatically at the end of evaporation or at key points during
the evaporation process. Alternatively, the condenser can be defrosted or drained manually
via the evaporator Select Run screen.
This manual will guide you through the requirements for setting up, operating and
maintaining the system. It will facilitate the most efficient procedures to protect your
product’s integrity and ensure optimum performance at all times.

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Safety
Warnings and cautions
This symbol is used within this manual to highlight warnings and cautions.
Warning: highlights a risk of personal injury or material damage.
Caution: highlights a risk of material damage.
Risk assessment
Caution: Carry out a risk assessment for each solvent before use. Consider the
possible consequences of applying vacuum to solvent mixtures, especially
organic solvents. For example, H2O2 gives off O2 under vacuum, creating an
oxygen rich atmosphere.
Precautions
Observe the following safety precautions when using the evaporation system:
Only operate the system in a ventilated space
Make sure exhaust / ventilation hoses are securely fitted and take solvent vapours away
from the equipment and from the operator
Only use sample holders and / or swings that are approved for use by Genevac
Do not leave the system unevenly loaded, someone may start it
Do not start or restart a system without checking it is evenly loaded
Do not place objects on top of the system
Check the condenser has been drained before starting the system
Refer to the Genevac published list of acceptable solvents for solvent compatibility
information
Clean up any solvent spillage immediately
Dispose of waste solvents in accordance with local environmental regulations.
Caution: Incorrect loading may result in damage to samples and to the system.

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Only allow users who are familiar with all the issues outlined in this User Manual to operate
the equipment. If personnel lack the training or experience to comprehend the hazards that
can arise when operating the system, do not allow them to use it; personnel without such
training require thorough instruction. The information contained within this User Manual may
form the basis of such training.
Any maintenance or repair of this product, other that that which is detailed in this user
manual, must be carried out by Genevac personnel (or approved representatives of
Genevac) using only Genevac approved spare parts.
Combustible solvents
Warning: Risk of vapour ignition. Only operate the system in a well ventilated
environment and consider safety when evaporating any combustible solvents.
Genevac's position regarding the evaporation of such solvents, particularly with
respect to the European ATEX directive, is available on our website, at
www.genevac.com, or from your local Genevac representative.
Electrical earthing
Warning: Risk of electric shock. This equipment must be earthed. The
evaporation system is a safety class I product according to IEC classification. It
must never be used with any interruption to the safety earth conductor. It is an
installation class II product and is intended to operate from a normal single-phase
supply.
Strong acids
Caution: Do not attempt to evaporate, HCl, thionyl chloride, or other acid
chlorides.
Low auto ignition point solvents
Warning: Risk of vapour ignition. Do not attempt to evaporate di-ethyl ether,
pentane or other low auto-ignition solvents.

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Options
Standard equipment
Genevac HT-24 evaporators feature the following equipment as standard:
Dri-Pure®(including Variable Dri-Pure) which eliminates sample bumping and prevents
associated problems of sample loss and cross contamination
SampleGuard™ for accurate sample holder temperature control
Onboard data logging –files can be transferred via a USB data key (not included)
Remote door open, the evaporator doors can be opened to clear fumes before opening
the fume hood doors.
In addition, the following optional specifications can be added at the time of ordering.
Consult your Genevac representative for advice on any other requirements.
Auto defrost and drain
AD&D enables the uninterrupted evaporation of mixtures of solvents with dissimilar boiling
points, including HPLC fractions. The system facilitates the use of the Genevac
LyoSpeed™ fast lyophilisation process.
High power lamps
High power Coolheat IR lamps can significantly reduce evaporation times when evaporating
solvents with high latent heat of evaporation (such as water, methanol or ethanol).
Fume hood
Fume hood for connection to laboratory fume extraction system.

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Sample holders
A wide range of swings and sample holders is available to cover plate, tube, vial and flask
formats, as well as the revolutionary Genevac SampleGenie™system. The illustration
shows just a few of the sample holders available.
Sample holders are manufactured in solid aluminium and are anodised to improve heat
absorption. The contact area is maximised to provide good physical support, optimum heat
transfer and even heat distribution for drying loads.
For further details of sample holders, contact your local Genevac representative to request a
Genevac Accessories Brochure or visit the Genevac web site at www.genevac.com.

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Basic Operation
Power up / down
Before switching the system on, make sure 3 x mains power leads are connected and the
mains power is switched on. If the EMERGENCY STOP button has been pressed, push and
twist to reset it, the emergency stop should not be used to power the system down on a
regular basis.
Note: The workstation power switch can be left on at all times. Power distribution to the
entire system, can be controlled the evaporator power switches.
To power up the system, switch on the evaporator power switches.
Each evaporator powers up in stand-by mode, the LCD screen shows the evaporator
software version. To make each evaporator operational, press the green START button on
the evaporator keypad.
Workstation power switch
Evaporator power switches
Three power leads

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The vacuum pump starts and the SELECT RUN menu appears on the evaporator
LCD screen. The condenser also powers up and begins to cool down to its operating
temperature. The system will not allow an evaporation run to commence until the condenser
reaches 0°C; if an attempt is made to start, the run will commence once operational
temperature has been reached.
To power down, enter the Select Run screen and press the STOP key. Press Y on the
evaporator keypad when prompted:
Note: If a pump purge sequence begins, allow this to finish before switching off the
evaporator power switch.

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Opening / closing the door
To open the door, press the OPEN CHAMBER # button
on the workstation control panel. If necessary, wait for
fumes to clear before opening the fume hood doors.
To close and lock the door, hold the door in the closed
position and press and hold the CLOSE button until a beep
sounds to indicate the door is locked.
Note: The door cannot be opened while the rotor is
turning or the evaporation chamber is below atmospheric
pressure.
To avoid the doors colliding, close the evaporator doors
before closing the fume hood doors.
Warning: Risk of hand entrapment between
the left-hand evaporator and the side of the
fume cupboard. Take care when opening the
evaporator door.
Stand-by mode
To enter stand-by mode when the system is idle (powered up but not evaporating), press
the evaporator STOP button. The LCD screen continues to operate with reduced brightness;
all other systems power down. Each evaporator can be placed in stand-by mode
independently.
To restart the system, press the START button.
Note: The system may continue to purge the vacuum pump for a preset time after entering
stand-by mode. The Purge Time remaining is indicated on the screen; do not power the
system down until the pump purge cycle has completed.

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Workstation controls
Workstation control panel
1. WASTE FULL: Lights-up when waste solvent container is full.
2. OPEN CHAMBER 2: Press to open evaporator 2 (right-hand-side evaporator).
3. ON indicator: Lights-up to indicate workstation is powered.
4. EMERGENCY STOP: Press to isolate the workstation and stop both evaporators in an
emergency.
5. POWER ON: Press to power up workstation.
6. OFF indictaor: Lights-up when EMEGENCY STOP has been pressed.
7. OPEN CHAMBER 1: Press to open evaporator 1 (left-hand-side evaporator).
5
6
7
1
2
3
4

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Evaporator controls
Frequently used function keys are positioned on the left hand side of the keyboard. The right
side illuminates to accept input for programming via the Run data screen when required.
1. LCD screen: Shows graphical and numerical data.
2. MENU: Press to display the User Menu screen, only operates when the system is idle.
3. CLOSE / OPEN: Press and hold to lock / unlock the door.
4. LCD screen: Press to show a graph of the last completed run, use the cursor direction
keys to show different segments in time.
5. SETTINGS: Press to display the Run Data screen for the selected run.
6. Keypad: Enter data for setting up or editing runs.
7. Evaporator power switch: Controls distribution of power to the entire system.
8. Service Panel: Vent inlet and electrical connections to vacuum pump and condenser.
9. USB port: Transfer data (methods or log files) via USB data key.
10. Service port: For use by service engineers only.
11. START: Press once to start the selected run or to execute the highlighted option.
12. STOP: When evaporator is operating, press once to stop; when evaporator is idle,
press once to enter standby mode.
13. Cursor direction keys: Scroll up and down, move around menu options on the
display.
1
4
11
5
6
7
9
8
2
3
12
13
10

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LCD screen
The illustration shows the LCD screen as it appears during an evaporation run, the graphical
and numerical information shown relates to evaporation progress.
Other menu screens appear during different operations and are discussed later in this User
Manual.
Run log screen shortly after evaporation has started
1. RUN: Currently selected method number.
2. Status: shows current evaporator status.
3. Graphical data: Shows progression of evaporation run, with data for Channel 1
(temperature) Channel 2 (temperature), Pressure and Spin Rate.
4. Messages and instructions: Provided instructions if user intervention is required, or
shows summary of current evaporator status.
5. Numerical data: Shows temperatures of evaporation chamber, sample holder, sample,
and condenser; also shows pressure, rotor speed and time elapsed or remaining.
6. ALARMS: Information or warnings regarding errors that occur during operation are
shown here.
2
3
6
1
5
4

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SampleGuard™
The SampleGuard transmitter has two input connectors for SampleGuard probes, these
must be connected for the SampleGurard system to function correctly. The numbers
1 and 2,embossed on the side of the SampleGuard transmitter, correspond to the
SampleGuard channels shown on the LCD screen. The third connector is for a stuffer plug
which should not be removed (this is for use by service engineers only).
The channel 1 temperature probe must always be used, it controls the temperature of the
sample holder.
The channel 2 temperature probe monitors the temperature of a sample. Runs that use
Sample Detection for the automated heat-off or end-of-run options, depend on monitoring
the evaporative cooling effect on samples, therefore these runs require the channel 2
temperature probe to be used. For runs that use Heat flow or timed auto shut down, the
use of SampleGuard channel 2 is optional.
SampleGuard probes are available in lengths to suit different types of sample holder.
Caution: Risk to sample integrity; make sure the probes are long enough to allow
the sample holder to swing
channel 1: insert the probe into the small hole (which is provided for this purpose) in
the sample holder.
channel 2: insert the probe into one the central sample tubes (or wells) making sure the
probe rests against the bottom of the tube. During evaporation, the probe is held firmly in
place by centrifugal force.
Channel 1 probe
inserted in hole
in sample holder
SampleGuard
transmitter
Channel 2 probe
in central well of
sample holder
Note: The illustration shows an HT-4X rotor; the same principals apply to HT-8 and HT-12
rotors.
Caution: Risk to sample integrity; the programmed SampleGuard Control
Temperature can only be maintained correctly if the channel 1 SampleGuard
probe is placed in the holder block as shown.
Note: Significant solvent cooling occurs during evaporation so samples in the central wells
can experience a thermal drain effect due to the greater number of surrounding samples.

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For this reason it is advisable to position the channel 2 sample probe near the centre of the
holder as these samples can take longest to dry.
The thermal drain effect is particularly prevalent when using microtitre plates. Under these
conditions, dryness may occur in the outer wells whist the central samples remain in solution
or frozen. The use of Genevac Heat Transfer Plates reduces the effect by providing an even
heat transfer across the plate. For details of the range of heat transfer plates that are
available, contact your local Genevac representative to request a Genevac Accessories
Brochure or visit the Genevac website at www.genevac.com.
Selecting a run
From the SELECT RUN screen, use the cursor direction keys to move the white band up
or down the screen.
Cursor direction keys
The screen displays ten runs at a time and the next ten runs appear when the white band
scrolls off the top or bottom of the screen. Up to 100 runs can be stored in total.
Move the white band until it required run is highlighted.
.
Starting a run
With the required run highlighted on the SELECT RUN screen, press the START button to
start an evaporation using the selected run.
Note: If the system is not ready, a message appears near the bottom of the screen, the run
will start once the system becomes ready.

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Run in progress
The Run Log screen provides data relating to evaporation progress, information is
presented in numerical and graphical form.
During evaporation, the effect of evaporative cooling (on the sample being monitored) can be
seen in the divergence of the SampleGuard channel 1 and channel 2 traces. When the
traces begin to converge, this indicates the sample is approaching dryness.
Run parameters can be changed whilst the evaporator is operating, refer to Advanced
Operation: Setting –up a run.
Stopping a run
To manually stop an evaporation run, press the STOP button. The evaporator vents to
atmospheric pressure, once a safe pressure is reached the rotor begins to slow down. The
door cannot be opened until the rotor is stationary and the system is at atmospheric
pressure.
When the rotor comes to a stop, a short sequence of beeps sounds to inform the user (this
repeats periodically).

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End of run
This screen appears after the run finishes.
Select Skip, Drain or Defrost using the numeric keys indicated.
Skip: The evaporator returns to the Run Select screen.
Drain: The system opens the drain valve and pumps any waste solvent into the waste
solvent container.
Defrost: Enter the defrost menu. Select START to begin the defrost cycle.
The evaporator defrosts the condenser until solvent defrost is automatically detected.
Alternatively, press the STOP key to end the defrost cycle.