Genevac EZ-2 Series User manual

T
Th he e G Ge en ne ev va ac c E EZ Z- -2 2 S Se er ri ie es s
P
Pe er rs so on na al l E Ev va ap po or ra at to or r
U
Us se er r M Ma an nu ua al l
Issue 1-6 –October 2008
Part Number 04-5359

04-5359 Issue 1-6 –October 2008
General Information
This manual has been produced to assist in the daily running and routine maintenance of the Genevac
Evaporator.
Amendment Control Form
Only the affected pages and the Front Page will display the Revision Number all others will stay at initial issue.
Revision
Number.
Issue and Reason for Change Date Issued
2 Introduction of Inert Gas Purge (IGP). Additional advice regarding the use
of H
y
dro
g
en
p
eroxide. 25 August 2006
3 Minor updates 13 February 2007
4 WARNING regarding lamp replacement on EZ-2 with Inert Gas Purge 26 April 2007
5 Information regarding ice formation and thawing in Condenser Jar 16 January 2008
1-6 Update U.S. address 24 October 2008
These instructions are subject to change without notice.
No part of these instructions may be reproduced in any form or be processed, duplicated or distributed by
electronic or optical means without the written permission of Genevac Limited.
All rights reserved. © Genevac Limited
These operating instructions should be read before you use the Genevac EZ-2 Series Personal Evaporator.
Keep them near the system for easy reference.
Your attention is drawn in particular to Page (vi), Paragraph 2.2, Safety.

04-5359 Issue 1-6 –October 2008 Page i
CONTENTS
Amendment Control Form
1INTRODUCTION
2SAFETY AND MAINTENANCE NOTES
2.1 Symbols
2.2 Safety
2.3 Collection jar – fitting and removing
2.4 Cleaning
2.5 Genevac Evaporators and Combustible Solvents
2.6 Safe loading of rotor
2.7 Lid operation
2.8 Limitations of use
SECTION 1 GENERAL
Chapter 1 UNPACKING AND SETTING UP
Flight Case.
Consumer Packaging
Chapter 2 QUICK START
A5 Quick reference card
Chapter 3 DETAILED OPERATION
SOLVENT LIMITATIONS
Evaporation of Acid Chlorides
Evaporation of Di-ethyl ether
Evaporation of High Boiling Point Solvents
Solvent Group Guide – EZ-2 & EZ-2plus (Quick Guide)
Maximum Run Times
Low Solvent Volumes
Solvent Group Guide – EZ-2 & EZ-2plus (Complete)
Solvent Group Guide – EZ-Bio (Complete)
Power Up
Starting a Run
Pre-heat / Pre-cool Stage
Changing the Run
Setting Run Time
During a run
Pausing a Run
Stopping a Run
Shutdown
Defrosting
Out of Balance
Inert Gas Purge
SECTION 2 FAULT FINDING / DIAGNOSTICS
Safety Interlocks and Errors
Audible Warnings
Other Troubleshooting
User Vacuum Test

Genevac EZ-2 Series Personal Evaporator – User Manual
04-5359 Issue 1-6 –October 2008 Page ii
SECTION 3 MAINTENANCE
Chapter 1 Software
1Introduction
2Safety and Maintenance Notes
3Special tools and equipment
4Preparation
5Uploading
6Testing
7Errors
8Completion
Chapter 2 Drive Belt
1Introduction
2Safety and Maintenance Notes
3Special tools and equipment
4Removal
5Refitting
6Testing
7Completion
Chapter 3 Fuses
1Introduction
2Safety and Maintenance Notes
3Special tools and equipment
4Removal
5Refitting
6Testing
7Completion
Chapter 4 Lamp Unit
1Introduction
2Safety and Maintenance Notes
3Special tools and equipment
4Removal
5Refitting
6Testing
7Completion
Chapter 5 Lamp Glass Assembly
1Introduction
2Safety and Maintenance Notes
3Special tools and equipment
4Removal
5Refitting
6Testing
7Completion
Chapter 6 Lid Seal
1Introduction
2Safety and Maintenance Notes
3Special tools and equipment
4Removal
5Refitting
6Testing
7Completion

Genevac EZ-2 Series Personal Evaporator – User Manual
04-5359 Issue 1-6 –October 2008 Page iii
Chapter 7 Pump
1Introduction
2Safety and Maintenance Notes
3Special tools and equipment
4Removal
5Refitting
6Testing
7Completion
Chapter 8 Pump Head Components
1Introduction
2Safety and Maintenance Notes
3Special tools and equipment
4Removal
5Refitting
6Testing
7Completion
SECTION 4 TECHNICAL SPECIFICATION
Mechanical Data
Vacuum system
Temperature controls
Condenser Data
Solvent compatibility
Dimensions
Inert Gas supply
Power Supplies
Power Consumption
Environment
Operating
Storage
Emissions
EC DECLARATION OF CONFORMITY
SAFETY
WARRANTY STATEMENT
USEFUL INFORMATION

Genevac EZ-2 Series Personal Evaporator – User Manual
04-5359 Issue 1-6 –October 2008 Page iv
QUICK REFERENCE GUIDE
LOCATION SLOT
CONTROL PANEL
LID
POWER SWITCH
COOLING VENTS
CONDENSER JAR
CATCH POT
LAMP ACCESS PANEL
HAND HOLD
HAND HOLD
1 Introduction
Using the most advanced proven technology in evaporation science, the EZ-2 Series has been designed
specifically for solvent removal in life science research, be that concentration of samples or complete
drying. Genevac’s fifteen years of experience in vacuum engineering has resulted in the creation of a
compact sample concentrator combining great performance, ease of use greater than a rotary evaporator
and compatibility with all commonly used solvents and acids.
The innovative design of the EZ-2 presents real advantages within parallel synthesis and medicinal
chemistry, as well as for general-purpose solvent removal needs in life science laboratories. At the heart of
the evaporator are a robust, solvent resistant, oil free pump from ILMVAC, and the high efficiency, defrost
free, SpeedTrap, designed by Genevac engineers, which traps all solvents as liquids, even water.
There are three EZ-2 models, Standard, Plus and Bio:
•The Standard (EZ-2) is suitable for water and volatile solvents.
•The Plus (EZ-2plus) is recommended for more difficult solvents with higher boiling points, as it is
equipped with chamber heating and a more powerful pump.
Either model can be specified with resistance to chlorinated acids.
•The Bio (EZ-Bio) is suitable for low to medium boiling point solvents in the range up to 120°C. There
are pre-programmed methods for DNA purification and extraction, oligosynthesis, protein purification
and removing aqueous mixtures. There is even a special method for HPLC fractions such as
Acetonitrile/water mixtures.
Fig 1

Genevac EZ-2 Series Personal Evaporator – User Manual
04-5359 Issue 1-6 –October 2008 Page v
The use of the high-efficiency solvent resistant Teflon diaphragm pump, which is integral to the evaporator,
saves you space and provides quiet, efficient control of the vacuum. Combined with a new glass jar
condenser that’s easy to empty, the “Next Generation” EZ-2 Series is our most compact and reliable
sample evaporator yet.
Fig 2 - EZ-2plus Control Panel
Compared with a typical rotary evaporator, the EZ-2 Series can process many more samples per unit time.
Simply select the solvent type and the maximum allowable sample temperature. Smart evaporation
software in the EZ-2 Series does the rest for you; setting the vacuum conditions and gradient, whilst
controlling the heater lamp and automatically stopping the run once the samples are dry. True walk-away
automation so simple, any one can use it with confidence.
Genevac’s patented sample protection systems prevent bumping and overheating of delicate samples,
whilst ensuring a smooth fast evaporation of the solvent, even with difficult mixtures. A wide selection of
popular sample holders accommodate most common sample formats including round-bottom flasks up to
500ml, tubes up to 160mm long, custom reaction blocks and, of course, shallow- or deep-well microplates.
For real peace of mind with thermally labile samples, the EZ-2 Series automated dryness detection and
auto shutdown software is always guarding your samples.
Sample evaporation has never been easier or quicker!

Genevac EZ-2 Series Personal Evaporator – User Manual
04-5359 Issue 1-6 –October 2008 Page vi
2 Safety and Maintenance Notes
2.1 Symbols
The following safety symbols are used throughout this manual and can be found on the equipment. The
definitions and scope of each symbol is as described below.
WARNING
THIS SYMBOL INDICATES HAZARDS THAT CAN LEAD TO SERIOUS MATERIAL
DAMAGE OR POTENTIAL SERIOUS INJURY.
THIS SYMBOL MEANS THAT YOU SHOULD NOT TOUCH SURFACES IN THESE
AREAS. THESE SURFACES CAN BE VERY HOT AND MAY LEAD TO BURNS.
This symbol indicates that you should refer to the relevant part of the User Manual for
additional information.
THESE 2 SYMBOLS COMBINED INDICATES HAZARDS THAT CAN LEAD TO
SERIOUS MATERIAL DAMAGE OR POTENTIAL SERIOUS INJURY AND THAT
YOU SHOULD READ THE RELEVANT PART OF THE USER MANUAL.
This symbol provides information about hazards that can be harmful to your health
or lead to material damage.
This symbol provides information about technical requirements, which if not followed, can
lead to malfunctions, inefficiency and reduced productivity.
This symbol indicates that there may be a risk to sample integrity.
2.2 Safety
Please be assured that sudden boiling of solvent within the condenser jar is normal and it will re-
condense. You may also see ice forming in the jar, this is normal under certain conditions.
The condenser jar is only to be emptied at the end of a run when the evaporator has stopped but not
when DEF is displayed.
If ice forms in the Condenser jar, allow it to thaw naturally. Do not use implements to try and break the
ice as this may damage the jar. Cold water may be added to the jar to accelerate the thawing process.
BEFORE OPERATING THE SYSTEM, IT IS IMPORTANT THAT THE FOLLOWING NOTES
ARE READ TO ENSURE THAT THE IMPLICATIONS TO THE SAFETY OF PERSONNEL
OPERATING THE SYSTEM AND FOR THE PROTECTION OF SAMPLE INTEGRITY ARE
UNDERSTOOD.
CARE IS TO BE TAKEN WHEN REMOVING THE COLLECTION JAR, AS IT IS HEAVY,
ESPECIALLY WHEN FULL OF SOLVENT.
INSPECT THE COLLECTION JAR BEFORE EVERY USE FOR DAMAGE, CRACKS ETC.
DO NOT ALLOW TO FILL ABOVE “MAX” LINE AS IT MAY CAUSE DAMAGE TO THE PUMP
AND STOP CONCENTRATION.
EMPTY THE COLLECTION JAR AFTER EVERY RUN.
REFER TO LIST OF ACCEPTABLE SOLVENTS BEFORE USE

Genevac EZ-2 Series Personal Evaporator – User Manual
04-5359 Issue 1-6 –October 2008 Page vii
Samples in the chamber are subjected to accelerations of up to 500G with a maximum load capacity of 1.5 kg
per swing.
Do not place any objects on top of the evaporator during a run
The following precautions should always be observed:
Ensure the catchpot or an alternative exhaust condenser is fitted prior to operation of the system.
Solvents condense in the condenser jar and catchpot (and any exhaust condenser when used). These
solvents should be disposed of appropriately.
Solvent vapour is present in the exhaust line, this line should be run to a safe extraction source. This is
particularly important if HCl or other acids are being used
Solvents can condense in the exhaust line and suitable precautions should be taken when working on the EZ-2.
This is particularly important if HCl or other acids have been used.
2.3 Collection jar – fitting and removing
To fit the jar, position so that the handle is to the front, lift the jar and rotate to the right a quarter turn until
resistance is felt.
The collection jar is removed by rotating the jar to the left a quarter turn.
•Always ensure that the condenser jar is emptied before every run.
•Clean the condenser jar at regular intervals.
2.4 Cleaning
Clean the lid, lid seal, exterior paintwork and inside of the chamber with a soft, lint free cloth using one of the
following:
Detergent solution - e.g. fairy liquid
Bleach solution (if using biological agents) – On inside of chamber and underside of lid ONLY.
Methanol
Ethanol
2.5 Genevac Evaporators and Combustible Solvents
Please note it remains the responsibility of the user to consider safety when evaporating any combustible
solvents and ensure the system is placed in a well ventilated environment. Genevac's position regarding
evaporation of such solvents, particularly with respect to the European ATEX directive, is available on our
website or from your local sales representative.

Genevac EZ-2 Series Personal Evaporator – User Manual
04-5359 Issue 1-6 –October 2008 Page viii
2.6 Safe loading of rotor
Never exceed the maximum load capacity of 1.5 kg per swing.
Balance sample holders to within 10g (approximately).
Locate tubes correctly in tube holders.
Load tube holders in balanced configurations.
Distribute tubes in sample holders symmetrically in both directions.
Locate sample blocks correctly in sample swings.
Before starting or restarting a run, check the following:
Ensure that both sample holders swing freely by swinging them by hand after loading.
The white PTFE washers located on each rotor pin are in place and in good condition.
All tube plates are correctly located.
Use only tubes that are able to withstand significant loads. Medium or thick wall Borosilicate glass tubes with a
wall thickness of no less than 1.2mm are recommended.
Do not use worn or scratched tubes or vials.
Do not load tubes or vials into sample holders other than those types that have been approved by Genevac Ltd.
Do not use sample holders that have not been supplied with this system without consulting Genevac Service.
2.7 Lid operation
The lid has an electrical lock and is opened and closed manually after pressing the LID unlock button.
There is a safety device fitted that prevents the lid from being unlocked and opened whilst the rotor is rotating
or until the chamber pressure has equalised.
2.8 Limitations of use
Accuracy of IR Sensor is dependant upon condition of Sample Holders and Swings. To ensure
correct operation, Sample Holders and Swings are to be kept clean and free from contamination
and corrosion.
Your evaporating system is unsuitable for use under the following circumstances:
•With strong mineral acids such as HCl and HBr at all concentrations, unless specifically built to order.
EVAPORATING DIETHYL ETHER AND SIMILAR LOW AUTO-IGNITION SOLVENTS WITHOUT
A GENEVAC INERT GAS PURGE SYSTEM FITTED TO THE EVAPORATOR AND PUMP.
•For use as a pressure vessel.
Genevac Ltd will
accept no
responsibility for any
loss or damage
incurred by improperly
or excessively loaded
rotors.

Genevac EZ-2 Series Personal Evaporator – User Manual
04-5359 Issue 2 – July 2006 Section 1
SECTION 1
GENERAL
Chapter 1 UNPACKING AND SETTING UP .........................................................................................................
Chapter 2 QUICK START......................................................................................................................................
Chapter 3 DETAILED OPERATION ......................................................................................................................

Genevac EZ-2 Series Personal Evaporator – User Manual
04-5359 Issue 1-6 –October 2008 Section 1 Chapter 1 - Page 1
Chapter 1
UNPACKING AND SETTING UP

Genevac EZ-2 Series Personal Evaporator – User Manual
04-5359 Issue 1-6 –October 2008 Section 1 Chapter 1 - Page 1
Flight Case.
1. Undo 4 quick release fasteners.
2. Carefully remove lid.
Refer to photographs on Pages 2 to 4 inclusive of this Chapter for aditional guidance.
3. Position base and EZ-2 near to operating bench / location.
4. With assistance (3 people total) lift EZ-2 from base and position where required on bench.
5. Ensure that there is 50mm clearance from edge of bench and other equipment.
6. Refit lid and store flight case.
7. Check contents of accessories container.
8. Fit inlet filter.
9. Fit condenser jar.
10. Fit Catchpot.
DURING ITEM 11. THE EXHAUST HOSE MUST BE FITTED; THIS HOSE MUST THEN BE
DUCTED TO A SUITABLE EXTRACTION UNIT/AREA OR A FUME HOOD.
11. Connect the long exhaust hose (supplied) and duct into fume hood. This is particularly important if HCl
or other acids are being used.
To maintain a solvent vapour free environment around your EZ-2, we suggest that you
do not use free space within the hood for storage of vessels containing solvents or
acids. Users should also ensure that tubing leading to and from the solvent condenser,
leads solvents and solvent vapours away from the machine in a safe manner. In
particular, the exhaust tube must be fitted to the outlet from the condenser and lead
away to the laboratory fume extraction system. This will ensure that any residual vapour
which passes through the condenser and pump does not cause damage to your system.
12. Connect mains supply lead (supplied).

Genevac EZ-2 Series Personal Evaporator – User Manual
04-5359 Issue 1-6 –October 2008 Section 1 Chapter 1 - Page 2
Consumer Packaging
1. Before signing for package or before opening case, check for damage.
(If there is, contact Genevac service):
UK Service: [phone]
USA Service: (1) 845-267-2211
2. Remove clips that retain the lid, and remove lid.
3. Check contents against packing list.
4. Remove the screws from the timber sleeve and carefully lift clear.
5. Position base and EZ-2 near to operating bench / location.
6. With assistance (3 people total) lift EZ-2 from base using hand-holds and position where required on to
bench.
LEFT HAND SIDE HAND-HOLDS
RIGHT HAND SIDE HAND-HOLDS

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7. Ensure that there is 50mm clearance from edge of bench and other equipment. (The system can be
easily moved by placing paper or similar under feet. If this is employed, ensure all traces are removed
on completion.)
8. Remove accessories containers and check contents against packing note.
9. If not already fitted, fit inlet filter.
10. Fit CatchPot (Not on EZ-Bio – refer to separate sheet supplied with evaporator).

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04-5359 Issue 1-6 –October 2008 Section 1 Chapter 1 - Page 4
11. Fit Condenser Jar.
DURING ITEM 12. THE EXHAUST HOSE MUST BE FITTED; THIS HOSE MUST THEN BE
DUCTED TO A SUITABLE EXTRACTION UNIT/AREA OR A FUME HOOD.
12. Connect the long exhaust hose (supplied) and duct into fume hood. This is particularly important if HCl
or other acids are being used.
To maintain a solvent vapour free environment around your EZ-2, we suggest that you
do not use free space within the hood for storage of vessels containing solvents or
acids. Users should also ensure that tubing leading to and from the solvent condenser,
leads solvents and solvent vapours away from the machine in a safe manner. In
particular, the exhaust tube must be fitted to the outlet from the condenser and lead
away to the laboratory fume extraction system. This will ensure that any residual vapour
which passes through the condenser and pump does not cause damage to your system.
13. Connect mains supply lead (supplied).