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  9. Gilson Extractman User manual

Gilson Extractman User manual

Gilson, Inc. | PO Box 620027 | Middleton, WI 53562-0027 | Tel: 608-836-1551 OR 800-445-7661 | Fax: 608-831-4451
Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 Villiers-le-bel, FRANCE
www.gilson.com | [email protected] | [email protected] | [email protected]
User’s Guide
LT310210-01
®
EXTRACTMAN® | User’s Guide
Description
7
1Description
EXTRACTMAN® leverages proprietary Exclusion-based Sample Preparation
(ESP™) technology to gently and quickly target proteins from a variety of
samples, including weakly-bound protein complexes that traditional isolation
techniques leave behind. The sliding handle processes up to four samples
simultaneously in as few as 30 seconds.
Handle
Slides to advance the bead capture strip
SLIDE
ReleaseMagnet
Position Indicator
Displays the position
of the releasemagnet
4-RowMicroplate
Holds sample solutions
and reagents; wellmoats
collect spillover
ReleaseMagnet
Disengages the
capturemagnets to release
paramagneticbeads Base
Positions the
4-rowmicroplate
and guides
the handle
ReleaseMagnet
Positioning Handle
Slides forward or
backward to position
the releasemagnet
Figure 1: EXTRACTMAN® Component Descriptions
EXTRACTMAN® | User’s GuideOperation |EXTRACTMAN®
Operation
17
Input, Wash, and Output Volumes
●The rst wellcolumn (460–500 µL) was designed for input wells.
●The second (230–275 µL) and third (100–110 µL) wellcolumns were
designed for input or wash wells.
●The fourth and fth (100–110 µL) wellcolumns were designed for
wash wells.
●The sixth (10–15 µL) wellcolumn was designed for output wells.
NOTE
Well volumes may vary because the formation
of stable, prominent menisci may require the
addition or subtraction of reagent, depending on
the surface tension of the reagent used.
3. Fill each subsequent well with wash or reagent solution.
NOTE
Wells should have prominent menisci to ensure proper
contact with the bead capture strip.
Figure 10: Input, Wash, and Output Well Locations
Input
(Blue)
Wash
(Red)
Output
(Green)
Input or Wash
(Purple)
460–500 µL 230–275 µL 100–110 µL 10–15 µL
Figure 11: Prominent Menisci on the 4-RowMicroplate
26 Operation |EXTRACTMAN®
Operation
Bead Size and Optimal Operation
The size of paramagnetic beads aects their time to pellet, suspension
dynamics, and performance.
Adjust Protocols for Large Paramagnetic Beads
Larger paramagnetic beads exert greater magnetic force than smaller
beads, resulting in a faster time to pellet. This dynamic may cause larger
paramagnetic beads to pull o the bead capture strip prematurely. This
can be avoided by raising the height of the bead capture strip. Refer to
Adjust the Collection Height on page 22.
Adjust Protocols for Small Paramagnetic Beads
Smaller paramagnetic beads exert less magnetic force than larger beads,
resulting in a slower time to pellet. This dynamic may result in longer
capture times. Gilson recommends waiting a few moments after the bead
discoloration in the wells has cleared before advancing to the next well
column.
Figure 22: Magnetic Intensity and Paramagnetic Bead Size
TIME TO PELLET
Figure 23: Paramagnetic Bead Size and Time to Pellet

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