Gilson PIPETMAN Neo P2N User manual

e Pipetting Comfort with Improved Comfort!
IMPORTANT NOTICE!
Do not lubricate this pipette.
The use of lubricant cancels
the warranty of this pipette.

2
1  INTRODUCTION
PIPETMAN Neo is a fully adjustable air displacement
pipette and is used with disposable tips.
PIPETMAN Neo is an evolution of the PIPETMAN Classic
to address the growing susceptibility of pipette users
to repetitive strain injuries. The implementation of new
features to improve pipetting comfort has no impact
on the legendary robustness, accuracy and precision of
PIPETMAN Classic.
 Six single channel models cover a volume range from
0.2 µL to 1000 µL.
 Two8andtwo12multichannel modelscoveravolume
range from 2 µL to 200 µL.
Contents Page
1  INTRODUCTION 2
2  PARTS CHECK LIST 3
3  DESCRIPTION 3
4  SETTINGTHEVOLUME 4
5  PIPETTING 5
6  GENERAL GUIDELINES FOR GOOD PIPETTING 9
7  ACCESSORIES 10
8  GLP FEATURES 11
9  TROUBLESHOOTING 11
10  LEAKTEST 12
11  MAINTENANCE 13
12 CLEANING AND DECONTAMINATION 17
13  SPECIFICATIONS 21
14  SPARE PARTS 23
EC DECLARATION OF CONFORMITY 26
WARRANTY 27

3
2  PARTS CHECK LIST
Just take a moment to verify that the following items
are present:
- PIPETMAN Neo,
- User's Guide,
- Safety bag,
- Certificate of conformity (including bar-code sticker).
3  DESCRIPTION
Please refer to the following chapters for a full description of
the different parts and functions of the pipette.
Color-coded
push-button
Thumbwheel
Tip-ejector button
Handle
Connecting nut
PIPETMAN DIAMOND® tip
Tip-holder Tip-ejector

4
4  SETTING THE VOLUME
Thevolumeofliquid tobe aspi-
ratedissetusingthevolumeter.
Color-coded push-button
Thumbwheel
Tip-ejector button
Handle
Connecting nut
PIPETMAN DIAMOND tips
Tip-holders
Tip-ejector
Ejector spacer
Cover
Ejector support
Ejector clip
Ejector lock
Model Color of volumeter numbers
Black Red Increment
P2N µL 0.01 µL 0.002 µL
P10N to P20N µL 0.1 µL 0.02 µL
P100N-P200N µL - 0.2 µL
P1000N 0.01 mL mL 0.002 mL
1
5
P2N
1.25 µL
2
0
7
5
P10N
7.5 µL
1
2
5
P20N
12.5 µL
P100N
0
7
5
75 µL
P200N
1
2
5
125 µL
P1000N
0
7
5
0.75 mL

5
The dials are colored either black or red to indicate the
position of the decimal point, depending on the model
(see examples).
The volume is set by turning
the thumbwheel or the push-
button. The push-button
makes it easier and quicker
to set volumes, especially
when wearing gloves. The
thumbwheel may be turned
usingonly one hand toslowly
reach the required setting.
To obtain maximum accuracy when setting the volume,
proceed as follows:
when decreasing the volume setting, slowly reach
the required setting, making sure not to overshoot
the mark.
whenincreasingthevolumesetting,passthe required
valueby1/3ofaturnandthenslowlydecreasetoreach
the volume, making sure not to overshoot the mark.
5  PIPETTING
Foroptimumperformance,useofPIPETMAN DIAMOND
Tipswith your PIPETMAN Neois strongly recommended.
These Tips, made from pure polypropylene have the
Gilson logo engraved on their collar, ensuring that you
haveagenuineGilson product.Plastictipsareforasingle
application – they must not be cleaned for reuse.
PIPETMAN Neo can also be used with the main tip brands.
Fitting the tips
Single channel models:
Tota newPIPETMANDIAMONDTip,pushthetip-holder
into the tip using a slight twisting motion to ensure a
firm, airtight seal.
For the P2N and P10N models,adual-positionadapter(plastic)
is required to fit DL10 tips (long tips) or D10 tips (short tips). The
metallicrodofthetip-ejectorisshapedsothattheadaptermay
be clipped to it in either of two positions.
Push-button Thumbwheeel

6
P2N and P10N models are delivered
withtheadapter inplace,positioned in
the longer slot, ready to use DL10 tips.
When D10 tips (which are shorter) are
used,theadaptermustberepositioned
in the shorter slot as follows:
Pull the adapter down from the
metallic rod.
Turn the adapter through 180°C.
Refit the adapter so that the end
of the metallic rod engages the
shorter slot of the adapter.
Multichannel models:
PIPETMAN DIAMOND Tip:
TIPACK™ and TOWERPACK™ – ROCKY RACK™
PIPETMAN DIAMOND Tips are best fitted from the pat-
ented ROCKY RACK available only in our TIPACKS and
TOWER PACKS.
ROCKY RACK is the dome-shaped part of the pack that
contains the tips. ROCKY RACK makes it easy to securely
fit the tips to a multichannel pipette, ensuring an airtight
seal on all channels without the need to use undue pres-
sure or to touch the tips.
The patented ROCKYRACK system available on
TOWERPACK and TIPACK makes it extremely easy
tot tipson a multichannelpipette.Tipswill not fall
o nor will they have to be manually positioned.
Ejector spacer
PIPETMAN DIAMOND Tip compatibility
8x20
12x20 DL10, DFL10, DF30, D200
8x200
12x200 D200, D300, DF100, DF200, DF300
Short tips Long tips

7
Pre-rinse the tips
Some liquids (e.g. protein-containing solutions and
organic solvents) can leave a lm of liquid on the inside
wall of the tip ; pre-rinse the tip to minimize any errors
that may be related to this phenomenon.
Pre-rinsingconsistsof aspiratingtherstvolumeofliquid
and then dispensing it back into the same vessel (or to
waste). Subsequent volumes that you pipette will have
levels of accuracy and precision within specications.

8
First stop
Second stop
Aspirate
Press the push-button to the first stop (this
corresponds to the set volume of liquid).
Holdthe pipette verticallyandimmersethe tip in the
liquid (see immersion depth table, page 10). Release
the push-button slowly and smoothly (to top posi-
tion) to aspirate the set volume of liquid. Wait one
second (time depends on model, see table) ; then
withdraw the pipette-tip from the liquid. You may
wipe any droplets away from the outside of the tip
using a medical wipe, however if you do so take care
to avoid touching the tip’s orifice.
Dispense
Place the end of the tip against the inside wall of the
recipient vessel (at an angle of 10° to 40°).
Press the push-button slowly and smoothly to the
rst stop.
Waitforat leasta second,thenpressthepush-button
to the second stop to expel any residual liquid from
thetip. Keepthepush-buttonpressedfullydownand
(while removing the pipette) draw the tip along the
inside surface of the vessel.

9
Release the push-button, smoothly. Eject the tip by
pressing rmly on the tip-ejector button.
6GENERALGUIDELINESFORGOODPIPETTING
Makesurethatyouop-
erate the push-button
slowly and smoothly.
When aspirating, keep
the tip at a constant
depth below the sur-
face of the liquid (refer
to the table).
Change the tip before
aspirating a dierent
liquid, sample, or reagent.
Change the tip if a droplet remains at the end of the
tip from the previous pipetting operation.
Each new tip should be pre-rinsed with the liquid
to be pipetted.
Liquid should never enter the tip-holder; to prevent
this:
- press and release the push-button slowly and
smoothly,
- never turn the pipette upside down,
- never lay the pipette on its side when there is liquid
in the tip.
If you use the same tip with a higher volume, pre-
rinse the tip.
For volatile solvents you should saturate the air-
cushionofyourpipettebyaspiratinganddispensing
the solvent repeatedly before aspirating the sample.
When the temperature of the liquid is dierent from
the ambient temperature, pre-rinse the tip several
times before use.
You may remove the tip-ejector (see Chapter 11 -
Maintenance) to aspirate from very narrow tubes.
After pipetting acids or other corrosive liquids
that emit vapors, remove the tip-holder, rinse the
Table - Immersion Depth
and Wait Time
Model Immersion Wait Time
Depth (mm) (seconds)
P2N 1 1
P10N 1 1
P20N 2-3 1
P100N 2-4 1
P200N 2-4 1
P1000N 2-4 2-3
8x20, 12x20 2-3 1
8x200, 12x200 2-3 1

10
piston, O-ring and seal with distilled water. For
the model P1000N, by using a specic tip holder
equipped with a lter, you can increase the life-
time of the piston (see Chapter 7 - Accessories).
Do not pipette liquids having temperatures above
70°C or below 4 °C.The pipette can be used between
+ 4 °C and + 40 °C but the specifications may vary
according to the temperature (refer to the ISO 8655-2
standard for conditions of use).
7  ACCESSORIES
To make pipetting more comfortable and more secure,
Gilson has developed several accessories:
To avoid the possibility of liquid running back into
the pipette, store the pipette vertically.
To identify or personalize your pipette, COLORIS™
clips are available:
WithTHE JIMMY™,handsfreemicrotubeopener,you
can open both snap-cap and screw-cap microtubes.
To protect the piston when pipetting corrosive liq-
uids, you can use a specic tip holder and lter for
the model P1000N:
CARROUSEL™ Pipette stand (7 pipettes) F161401
TRIO™ stand (3 pipettes) F161405
SINGLE™ pipette holder F161406
COLORIS™ clips (mixed colors set of 10) F161301
COLORIS™ clips (red, set of 10) F161302
COLORIS™ clips (yellow, set of 10) F161303
COLORIS™ clips (green, set of 10) F161304
COLORIS™ clips (blue, set of 10) F161305
COLORIS™ clips (white, set of 10) F161306
THE JIMMY™ (set of 3) F144983
Corrosion protection kit (tip holder + a bag of 10 filters) F144570

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8  GLP FEATURES
TheSerialNumberisengraved
on the body of the pipette. It
providesuniqueidentication
of your pipette and the date
of manufacture; Ex : AA 10369
The Bar Code on the box and
the certificate of conformity
provide traceability of your
pipette.
9  TROUBLESHOOTING
A quick inspection of the pipette may help you to detect
a problem.
You may downloadfromtheGilsonwebsite(www.gilson.com)
the “2 minute inspection”, which shows how to perform a quick
diagnosis of your pipette.
Before returning any pipette to your local Gilson
Service Center, ensure that it is completely
free of chemical, biological, or radioactive
contamination. Refer to Chapter 12 - Cleaning
and Decontamination. Please use the included
safetybagtoreturnthepipettetoyourlocalGilson
Service Center.
The following table may help you to identify and correct
the problem you might encounter.
Symptom Possible Cause Action
Pipette is Damaged tip-holder Replace the tip-holder*
leaking sample Worn O-ring or seal Replace both parts*
Pipette won’t Worn O-ring Replace both parts*
aspirate Damaged tip-holder Replace the tip-holder*
Connecting nut is loose Tighten connecting nut
Damaged or corroded piston Return pipette to supplier
Improper repair or assembly See Chapter 11 - Maintenance

12
* Not applicable for multichannel models
However, if you can’t solve the problem, contact your
Gilson representative.
Symptom Possible Cause Action
Pipette is Tip-holder is loose Tighten connecting nut*
not precise Connecting nut is loose Tighten connecting nut
Incorrect operator technique Operator training
Damaged or corroded piston(s) Return pipette to supplier
Damaged tip-holder(s) Replace the tip-holder*
Worn O-ring or seal Replace both parts*
Tips fall off or Low quality tips Use PIPETMAN DIAMOND tips
do not fit Damaged tip-holder(s) Replace the tip-holder*
correctly Damaged tip-ejector Replace tip-ejector
Ejector spacer is missing Mount the spacer on the tip- ejector
The ejector spacer is damaged Replace the ejector spacer
The tip-ejector is loose Assemble the tip-ejector properly
The ejector lock is misaligned Align theejector lock
10  LEAK TEST
This test may be performed at any time to check that
the pipette does not leak, especially after performing a
maintenanceordecontaminationprocedure. If a pipette
fails this test, replace the O-ring and seal. After making
surethatthepipette iscorrectlyreassembled,repeatthis
test (not applicable to multichannel pipettes).
FortheP2NtoP200N,8x20,12x20,8x200and12x200
models:
Fit a PIPETMAN DIAMOND Tip.
Set the pipette to the maximum volume given in the
specifications, and pre-rinse.
Aspirate the set volume from a beaker of distilled
water.
Maintain the pipette in the vertical position and wait
for 20 seconds.
If a water droplet appears at the end of the tip there
is a leak.
If you see no droplet, re-immerse the tip below the
surface of water.

13
Thewaterlevelinsidethetipshouldremainconstant;
if the level goes down there is a leak.
For the P1000N model:
Fit a PIPETMAN DIAMOND tip.
Set the pipette to the maximum volume given in
the specifications.
Aspirate the set volume from a beaker of distilled
water.
Maintain the pipette in the vertical position and wait
for 20 seconds.
If a water droplet appears at the end of the tip, there
is a leak.
11  MAINTENANCE
Routine maintenance will help keep your pipette in
good condition, ensuring a continued high level of
performance. Maintenance is limited to cleaning or au-
toclavingthepartsspecifiedunder Chapter12-Cleaning
and Decontamination or to replacing the push-button,
connecting nut, tip-ejector, tip-holder, seal and O-ring.
PIPETMAN Neo P2N and P10N should not be
disassembled,soyoumayonlyreplacethepush-button,
tip-ejector, dualposition tip-ejector andits adapter.With
these pipettes if the tip-holder is damaged, the piston
may also be damaged.
For multichannel models, the lower part should not be
disassembled:onlythepush-button,theconnectingnut
and the tip-ejector can be replaced.
After replacing any parts you should verify
the performance of your pipette following the
verification procedure available on the Gilson
website (www.gilson.com). If the pipette needs
to be readjusted, please contact your local Gilson
authorized Service Center.

14
Single channel models
Changing the Tip-ejector, to remove
Pushtheejection
button.
Pushlaterallythe
tip-ejector.
Slideandremove
the tip-ejector.
To ret
Push the ejection button.
Slide the tip-ejector along the tip-holder.
Clip the tip-ejector on the body of the pipette.
Changing the Tip-holder – no tools required
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it counter-
clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly, O-ring and seal.
Clean, autoclave, or replace the tip-holder.
Reassemble the pipette (refer to the figure, p 15).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
Servicing the Piston Assembly
You may remove the piston assembly for cleaning pur-
posesonly. Ifthepistonassembly is changed,the pipette
must be adjusted and calibrated in a Gilson authorized
Service Center.
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it counter-
clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly, O-ring and seal.
Clean and decontaminate the piston assembly.

15
Color coded push-button
Connecting nut
Seal
O-ring
Tip-holder
Tip-ejector
Piston assembly
The piston assembly must not be autoclaved.

16
Reassemble the pipette (refer to the figure, page 15).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
Changing the O-ring
The O-ring and seal are on the piston; they must not be
autoclaved, if worn or damaged in any way (chemical or
mechanical), they must be replaced.
The dimensions of the O-ring vary depending on the
model of pipette.
Remove the tip-ejector (see above).
Unscrew the connecting nut by turning it counter-
clockwise.
Carefully separate the lower and upper parts.
Remove the piston assembly, O-ring and seal.
Clean or replace the seal then the O-ring.
Reassemble the pipette (refer to the figure, page 15).
Tighten the connecting nut (turn clockwise).
Refit the tip-ejector (see above).
Multichannel models
ChangingtheTip-ejector
To remove the tip-
ejector, keep both
ejector locks de-
pressed.
Pull the tip-ejector
down.
To refit the tip-
ejector, gently re-
insertthetip-ejector
vertically into the
rails of the ejector
support.
Pull lightly on the
tip-ejector to check
the position.
Ejector lock
Tip-ejector
Ejector spacer

17
12  CLEANING AND DECONTAMINATION
PIPETMAN Neo is designed so that the parts normally in
contact with liquid contaminants, can easily be cleaned
and decontaminated. However, because the models P2N
and P10N contain miniaturized parts, it is best not to disas-
semble these pipettes yourself; please contact your local
Gilson authorized Service Center.
Cleaning
The pipette must be cleaned, as described below, before it is
decontaminated. Soap solution is recommendedforcleaning
PIPETMAN Neo.
Single channel models - External
Remove the tip-ejector.
Wipe the tip-ejector with a soft-cloth or lint-free
tissue impregnated with soap solution.
Wipe the entire pipette with a soft-cloth or lint- free
tissueimpregnated withsoap solution, to removeall
dirty marks. If the pipette is very dirty, a brush with
soft plastic bristles may be used.
Wipethe entirepipetteandthetip-ejectorwithasoft
cloth or lint-free tissue soaked with distilled water.
Refit the tip-ejector and allow the pipette to
dry.
Single channel models - Internal
The following components only can be immersed in a
cleaning solution: connecting nut, tip-ejector, tip-holder,
piston assembly, seal and O-ring.
Disassemble the pipette as described in the Chapter
11 - Maintenance.
Set aside the upper part in a clean, dry place.
Cleantheindividualcomponentsof thelowerpartof
the pipette using an ultrasonic bath (20 minutes at
You may refer to the decontamination procedure
available on the Gilson website (www. gilson.
com). Liquid must never enter the upper part
(handle) of any pipette.

18
50°C) or with a soft-cloth and brushes. Small round
brusheswithsoftplasticbristlesmaybeusedtoclean
the interior of the tip-holder.
Rinsetheindividualcomponentswithdistilledwater.
Leave the parts to dry by evaporation or wipe them
with a clean soft-cloth or lint-free tissue.
Reassemble the pipette as described in the Chapter
11 - Maintenance.
Multichannel models
The following components only can be immersed in a
cleaning solution: tip-ejector, ejector locks and ejector
spacer.
Remove the tip-ejector and the ejector spacer.
Immerse the tip-ejector, ejector locks and ejector
spacer in the cleaning solution or wipe them with
a soft-cloth or lint-free tissue impregnated with the
cleaning solution.
Rinse the components with distilled water.
Wipe the entire pipette with a soft cloth or lint-free
tissue impregnated with the cleaning solution.
Wipe it with distilled water.
Leave the parts to dry by evaporation or wipe them
with a clean soft-cloth or lint-free tissue.
Refit the tip-ejector as described in page 16 (Chang-
ing the tip-ejector).
Autoclaving
Single channel models
The upper part (body) and the piston assembly of the
pipette are not autoclavable. Only the following parts
maybeautoclaved:tip-ejector,tip-holderandconnecting
nut. The O-ring and seal are not autoclavable; they may
be cleaned or replaced with the one specified in Chapter
14 - Spare Parts.
Clean the parts to be autoclaved, especially the
tip-holder.
Put the parts in an autoclaving sack.
Autoclave for 20 minutes at 121°C, 0.1 MPa.

19
Check that the parts are dry before re-assembling
the pipette.
Setthepipetteasidetostabilizeatroomtemperature.
Multichannel models
The complete lower part can be autoclaved (20 minutes
at 121°C, 0.1 MPa).
The lower part has to be removed from the body.
To remove:
Keep the tip-ejector
button depressed,
Turn the ejector clip
counter-clockwise
and separate it from
the handle.
Unscrew the con-
necting nut turning
counter-clockwise.
Press push-button
to extract the head.
To autoclave:
Clean the parts
to be autoclaved,
especially the tip-
holders and the
ejector spacer.
Put the lower part in
an autoclaving sack.
Autoclave for 20
minutes at 121°C,
0.1 MPa.
Check that the auto-
clavedpartis drybe-
fore re-assembling
the pipette.
Setthe pipetteaside
to stabilize at room
temperature.

20
To t the lower part on the body,
Pressonthepush-button.
Insert the lower part into
the ejection rod. Release
the push-button.
Screwtheconnectingnut
turning clockwise.
Press on the tip-ejector
button.
Inserttheejectorclipand
release the tip-ejector
button.
ChemicalDecontamination
You may choose to decontaminate your pipette chemi-
cally,in accordance withyourownprocedures.Whatever
decontaminant you use, check with the
supplier of the
decontaminant that it is compatible with stainless steel
and the plastics used in the construction of the pipette:
PA (Polyamide), PBT (Polybutylene Terephtalate), PC
(Polycarbonate), PC/PBT (Polycarbonate/ Polybutylene
Terephtalate), POM (Polyoxymethylene), or PVDF (Poly-
vinylidene Fluoride).
Single and Multichannel - Upper Part (handle)
Wipe the upper part (handle) of the pipette with a
soft-cloth or lint-free tissue impregnated with the
chosen decontaminant.
Wipe the upper part of the pipette with a soft- cloth
or lint-free tissue soaked with distilled water or
sterile water.
Single channel - Lower Part (Volumetric module)
The following components only can be immersed in a
decontaminant solution: connecting nut, tip-ejector,
tip-holder, piston assembly, seal and O-ring.