Gilson PIPETMAN Classic P2 User manual

Quick Guide
EN
CLASSIC

2QUICK GUIDE | PIPETMAN® CLASSIC
Introduction
PIPETMAN® Classic is a fully adjustable, air-displacement
pipette that is used with disposable tips.
Eight single channel models cover a volume range from
0.2 µL to 10mL for many applications.
Parts Checklist
●PIPETMAN Classic
●PIPETMAN Classic Quick Guide
●Dual-position adapter (for P2 and P10 models only;
refer to Fitting the Tips on page 5)
●Safety bag
●Certificate of Conformity (including barcode
sticker)
GLP Compliant
The serial number is engraved on the body of the
pipette. It provides unique identification of your pipette
and the date of manufacture. For more details, refer to
PIPETMAN Classic User’s Guide LT801120 . Ex:QG10369
The barcode on the box and the Certificate of
Conformity provide traceability of your pipette.
Figure 1
Serial number location
NOTE
The updated line of PIPETMAN Classic
is identified with an underlined serial
number starting from QG.
Serial Number

3
PIPETMAN® CLASSIC |QUICK GUIDE
Description
Thumbwheel
Body or
handle
Specifications
PIPETMAN Classic is a high quality pipette that offers
excellent accuracy and precision. The data provided
in the Maximum Permissible Errors table on page 4
were obtained using PIPETMAN® DIAMOND Tips.
These values are guaranteed only when genuine
PIPETMAN® DIAMOND Tips are used.
Each pipette is inspected and validated by qualified
technicians in accordance with the Gilson Quality
System. Gilson declares that its manufactured
pipettes comply with the requirements of the
ISO8655 standard by type testing. The adjustment
is carried out under strictly defined and monitored
conditions (ISO8655-6).
Tip holder
PIPETMAN®
DIAMOND Tip
Figure 2
PIPETMAN® Classic single channel model
Connecting
nut
Color-coded
push button
Tip ejector
button
Tip ejector
Clip tip
ejector

4QUICK GUIDE | PIPETMAN® CLASSIC
Maximum Permissible Errors
The data provided in the following tables conform to
the ISO8655-2 Standard.
*P5000 and P10mL do not have tip ejectors.
NOTICE
Do not lubricate this pipette. The use of
lubricant cancels the warranty of this pipette.
VOLUME
(µL)
GILSON ISO 8655
SYSTEMATIC
ERROR (µL)
RANDOM
ERROR (µL)
SYSTEMATIC
ERROR (µL)
RANDOM
ERROR (µL)
P2 (P/NF144801) WITH D10 OR DL10 TIPS
0.2 ± 0.024 ≤0.012 ± 0.08 ≤0.04
0.5 ± 0.025 ≤0.012 ± 0.08 ≤0.04
1± 0.025 ≤0.012 ± 0.08 ≤0.04
2± 0.030 ≤0.014 ± 0.08 ≤0.04
P10 (P/NF144802) WITH D10 OR DL10 TIPS
1± 0.025 ≤0.012 ± 0.12 ≤0.08
5± 0.075 ≤0.030 ± 0.12 ≤0.08
10 ± 0.100 ≤0.040 ± 0.12 ≤0.08
P20 (P/NF123600) WITH D200 TIPS
2± 0.10 ≤0.030 ± 0.2 ≤0.1
5± 0.10 ≤0.040 ± 0.2 ≤0.1
10 ± 0.10 ≤0.050 ± 0.2 ≤0.1
20 ± 0.20 ≤0.060 ± 0.2 ≤0.1
P100 (P/NF123615) WITH D200 TIPS
10 ± 0.35 ≤0.10 ± 0.8 ≤0.3
50 ± 0.40 ≤0.12 ± 0.8 ≤0.3
100 ± 0.80 ≤0.15 ± 0.8 ≤0.3
P200 (P/NF123601) WITH D200 TIPS
20 ± 0.50 ≤0.20 ± 1.6 ≤0.6
100 ± 0.80 ≤0.25 ± 1.6 ≤0.6
200 ± 1.60 ≤0.30 ± 1.6 ≤0.6
P1000 (P/NF123602) WITH D1000 TIPS
100 ± 3.0 ≤0.6 ± 8.0 ≤3.0
500 ± 4.0 ≤1.0 ± 8.0 ≤3.0
1000 ± 8.0 ≤1.5 ± 8.0 ≤3.0
P5000* (P/NF123603) WITH D5000 TIPS
500 ± 12 ≤3 ± 40 ≤15.0
2500 ± 15 ≤5 ± 40 ≤15.0
5000 ± 30 ≤8 ± 40 ≤15.0
P10mL* (P/NF161201) WITH D10ML TIPS
1000 ± 30 ≤6 ± 60 ≤30.0
2000 ± 30 ≤6 ± 60 ≤30.0
5000 ± 40 ≤10 ± 60 ≤30.0
10000 ± 60 ≤16 ± 60 ≤30.0

5
PIPETMAN® CLASSIC |QUICK GUIDE
Setting the Volume
The volume of liquid to be aspirated is
set using the volumeter. The dials are
colored either black or red to indicate the
position of the decimal point, depending
on the model (refer to Figure 3).
The volume is set by turning the
thumbwheel or the push button. The push
button makes it easier and faster to set
volumes, especially when wearing gloves.
The thumbwheel may be turned to slowly
reach the required setting.
To achieve maximum accuracy when
setting the volume:
●When decreasing the volume setting, slowly reach the
required setting, making sure to not pass the setting.
●When increasing the volume setting, pass the
required value by 1/3 of a turn and then slowly
decrease to reach the volume, making sure to not
pass the setting.
MODEL COLOR OF VOLUMETERNUMBERS
BLACK RED INCREMENT
P2 µL 0.01 µL 0.002 µL
P10 to P20 µL 0.1 µL 0.02 µL
P100–P200 µL —0.2 µL
P1000–P5000 0.01 mL mL 0.002 mL
P10mL mL 0.1 mL 0.02 mL
Fitting the Tips
To fit a new PIPETMAN DIAMOND Tip, push the tip holder
into the tip using a slight twisting motion to ensure a firm
and airtight seal.
NOTE
For the P2 and P10 models, a dual-position
adapter (plastic) is required to fit DL10 tips
(longtips) or D10 tips (short tips). The metallic
rod of the tip ejector is shaped so that the
adapter may be clipped to it in either position.
P2 and P10 models are delivered with the
adapter in place ready to use DL10tips.
When D10 tips are used, the adapter must be
repositioned in the shorter slot as follows:
1. Pull the adapter down from the metallic rod.
2. Turn the adapter 180°.
3. Refit the adapter so the end of the
metallic rod engages the shorter slot of
the adapter.
Figure 3
Dial colors by
model
Short
tips
Long
tips
Figure 4
Dual-position
adapter for
P2 and P10
NOTE
Plastic tip ejector can be used
and ordered separately, (refer
to PIPETMAN Classic User’s
Guide LT801120 ).
NOTICE
Do not lubricate this pipette. The use of
lubricant cancels the warranty of this pipette.

6QUICK GUIDE | PIPETMAN® CLASSIC
Pre-Rinse the Tips
Some liquids (e.g., protein-containing solutions and
organic solvents) can leave a film of liquid on the inside
wall of the tip. Pre-rinse the tip to minimize any errors
that may be related to this phenomenon.
Pre-rinsing consists of aspirating the first volume
of liquid and then dispensing it back to waste.
Subsequent volumes you pipette will have levels of
accuracy and precision within specifications.
Aspirate
1. Press the push button to the first stop (this
corresponds to the set volume of liquid).
2. Hold the pipette vertically and immerse the tip in
the liquid (refer to PIPETMAN Classic User’s Guide
LT801120 Guidelines for Good Pipetting ) .
3. Release the push button slowly and smoothly
(to the top position) to aspirate the set volume
of liquid.
4. Wait one second. The time depends on the
model (refer to the table). Then, withdraw the
pipette tip from the liquid. You may wipe any
droplets away from the outside of the tip using a
medical wipe; however, if you do so, take care to
avoid touching the tip’s orifice.
Dispense
1. Place the end of the tip against the inside wall of
the recipient vessel (at an angle of 10° to 40°).
2. Press the push button slowly and smoothly to the
first stop.
3. Wait for at least a second, then press the push
button to the second stop to expel any residual
liquid from the tip. Keep the push button pressed
fully down and (while removing the pipette) draw
the tip along the inside surface of the vessel.
4. Release the push button smoothly. Eject the tip by
pressing firmly on the tip of the ejector button.
First stop
Second stop
Figure 5
Pipetting motion - aspirate and dispense
Rest position

7
PIPETMAN® CLASSIC |QUICK GUIDE
Maintenance
Routine maintenance will help keep your pipette in good
condition, ensuring a continued high level of performance.
Maintenance is limited to cleaning or autoclaving the parts
specified in the Cleaning and Decontamination chapter of
the PIPETMAN Classic User’s Guide (LT801120).
NOTICE
PIPETMAN P2 and P10 should not be
disassembled; you may only replace the
push button, tip ejector, and the dual
position adapter. With these pipettes if
the tip holder is damaged, the piston may
also be damaged. After replacing any
parts you should verify the performance
of your pipette following the verification
procedure available on the Gilson website
(www.gilson.com). If the pipette needs to
be readjusted, please contact your local
authorized Gilson Service Center.
Warranty
Gilson warrants this pipette against defects in material
under normal use and service for a period of three years
from the date of purchase.
This warranty shall not apply to pipettes which are
subject to abnormal use and/or improper or inadequate
maintenance (contrary to the recommendations
given in the user’s guide), including, but not limited
to pipettes which have been subjected to physical
damage, improper handling, or spillage or exposure to
any corrosive environment. This warranty shall also be
void in the event pipettes are altered or modified by any
party other than Gilson or its designates. Gilson’s sole
liability under this warranty shall be limited to, at Gilson’s
sole option, repair or replacement of any defective
components of pipettes or refund of the purchase price
paid for such pipettes. Routine cleaning, control and
recalibration are not covered under the warranty. The
replacement of wearing parts such as seals, O-rings,
broken pistons assembly, and broken tip holders are not
covered under the warranty.
NOTICE
Yearly routine maintenance is highly
recommended to keep your pipette in good
condition, ensuring a continued high level of
performance.
Web Resources
Visit Gilson’s website at www.gilson.com for:
●Service contact information
● PIPETMAN Classic User’s Guide LT801120 pdf
●Information about accessories, spare parts, and
related products
●Additional product information and special offers

www.gilson.com/contactus
LT801623/C | ©2019 Gilson, Inc. All rights reserved.