Gilson Pipetman Fixed User manual

User’s Guide
EN
FIXED

TABLE OF CONTENTS
TABLE OF CONTENTS |PIPETMAN® FIXED
TABLE OF CONTENTS
INTRODUCTION | 3
SPECIFICATIONS | 4
PIPETTING | 6
Aspirate | 6
Dispense | 6
Pre-Rinse | 7
User Adjustment | 7
ASSOCIATED TIPS | 9
RECOMMENDATIONS | 11
TROUBLESHOOTING | 12
SPARE PARTS | 14
WARRANTY | 15

Chapter 1
INTRODUCTION
PIPETMAN
®
Fixed volume
pipettes are available in
14 dierent models.
Together, they cover a
range of volumes from 2 to
5000 µL.
The pipetting mechanism
incorporates a highly
polished stainless steel
piston, a seal, and an O-ring.
The mechanism requires no
routine lubrication.
Tip ejector and disposable
tips mean no need to handle
tips, protecting the user from
contamination.
The tip holder, the
connecting nut, and the tip
ejector are auto,clavable for
20 minutes at 121°C and
0.1 MPa pressure.
The PIPETMAN Fixed
can be easily re-adjusted
in the laboratory, with
compensation for
variations in fluid viscosity
and density. A special
calibration key
is provided.
The nominal volume of
each model is shown on
the small label on the
push button.
PIPETMAN Fixed is in
accordance with ISO 8655
Standard.
INTRODUCTION
3
PIPETMAN® FIXED |USER’S GUIDE
Figure 1
PIPETMAN® Fixed
Tip ejector
button
Push button
Body
or
handle
Connecting
nut
Tip holder
Tip ejector
PIPETMAN
DIAMOND tip
Figure 2
Push Button
Top View

SPECIFICATIONS
4SPECIFICATIONS | PIPETMAN® FIXED
Chapter 2
SPECIFICATIONS
Maximum Permissible Errors
PIPETMAN Fixed is a high quality pipette that
oers excellent accuracy and precision. The figures
given in the “Gilson Maximum Permissible Errors”
table were obtained using “PIPETMAN DIAMOND
Tips”. These figures are only guaranteed using
genuine PIPETMAN DIAMOND Tips.
MODEL VO L*
GILSON ISO 8655
SYSTEMATIC
ERROR*
RANDOM
ERROR*
SYSTEMATIC
ERROR*
RANDOM
ERROR*
F2
(F123770) 2± 0.08 ≤0.03 ± 0.08 ≤0.04
F5
(F123771) 5± 0.10 ≤0.04 ± 0.125 ≤0.075
F10
(F123772) 10 ± 0.10 ≤0.05 ± 0.12 ≤0.08
F20
(F123604) 20 ± 0.20 ≤0.06 ± 0.2 ≤0.1
F25
(F123775) 25 ± 0.25 ≤0.07 ± 0.5 ≤0.2
F50
(F123778) 50 ± 0.40 ≤0.15 ± 0.5 ≤0.2
F100
(F123784) 100 ± 0.80 ≤0.25 ± 0.8 ≤0.3
F200
(F123605) 200 ± 1.60 ≤0.30 ± 1.6 ≤0.6
F250
(F123787) 250 ± 3.00 ≤0.75 ± 4.0 ≤1.5
F300
(F123788) 300 ± 3.50 ≤0.75 ± 4.0 ≤1.5
F400
(F123789) 400 ± 3.60 ≤0.80 ± 4.0 ≤1.5
F500
(F123790) 500 ± 4.00 ≤1.00 ± 4.0 ≤1.5
F1000
(F123606) 1000 ± 8.0 ≤1.3 ± 8.0 ≤3.0
F5000
(F123607) 5000 ± 30 ≤8 ± 40 ≤15.0
NOTE
The data given in the table conform to the
ISO 8655-2 Standard.
*All Values in Microliters

SPECIFICATIONS
5
PIPETMAN® FIXED |USER’S GUIDE
SPECIFICATIONS | PIPETMAN® FIXED
Each pipette is inspected and validated by
qualified technicians according to the Gilson
Quality System.
Gilson declares that its manufactured pipettes
comply with the requirements of the ISO 8655
Standard, by type testing. The adjustment is
carried out under strictly defined and monitored
conditions (ISO 8655-6):
●Basis of adjustment, Ex.
●Reference temperature, 20°C
●Relative humidity, 50%
●Barometric pressure, 101 kPa
●Use of distilled water
grade 3 (ISO 3696)
●Ten measurements for each test volume.

PIPETTING
6PIPETTING | PIPETMAN® FIXED
NOTE
Always fit a tip to your PIPETMAN before
aspirating any liquid.
Chapter 3
PIPETTING
Place a tip on the tip holder. Press the tip on firmly
using a slight twisting motion to ensure a positive,
airtight seal (refer to CHAPTER 4 - ASSOCIATED
TIPS, on page 9).
Aspirate
●Press the push button to
the first positive stop (A),
refer to Figure 3.
●Hold the pipette vertically
and immerse the tip
into the liquid (depth
immersion 2-4 mm).
●Release the push button
slowly and smoothly to
aspirate the liquid (B),
refer to Figure 3.
●Wait one second and then
withdraw the tip from the
liquid. Wipe any droplets
away from the outside of
the tip using medical wipe.
Dispense
●Place the end of the tip against the inside wall
of the vessel at an angle of 10 to 40 degrees.
Press the push button smoothly to the first
stop (C), refer to Figure 3. Wait one second.
Press the push button to the second stop to
expel any residual liquid from the tip (D), refer
to Figure 3.
●Keep the push button pressed fully down
and (while removing the pipette) draw the tip
along the inside surface of the vessel.
●Release the push button smoothly.
●Eject the tip by pressing the tip ejector button.
Figure 3
Pipetting Positions
NOTE
Avoid touching the orifice of the tip.
A B C
D

PIPETTING
7
PIPETMAN® FIXED |USER’S GUIDE
PIPETTING | PIPETMAN® FIXED
NOTE
Always fit a tip to your PIPETMAN before
aspirating any liquid.
User Adjustment
PIPETMAN Fixed is factory calibrated using
distilled water and very high precision balances.
The nominal value of the PIPETMAN Fixed may
be slightly adjusted to compensate for liquids of
dierent density or viscosity.
This adjustment to accommodate for density
or viscosity is limited to one full turn of the
calibration key in either direction, which equals:
± 1.0 µL (models F2 to F20)
± 4.0 µL (models F25 to F100)
± 10.0 µL (models F120 to F200)
± 40.0 µL (models F250 to F1000)
± 200.0 µL (model F5000)
Adjustments of the factory
calibration must only be performed
using a special calibration key (refer
to Figure 4, on page 8)
NOTICE
NOTE
The display is graduated in tenths of
the key revolution (letters A, B,... J) and
is read from left to right. Each small
graduation mark represents 0.2 of the
distance between each letter.
Pre-Rinse
Some liquids (e.g., protein–containing solutions
and organic solvents) can leave a film of liquid on
the inside the wall of the tip; pre-rinse the tip to
minimize any errors that may be related to this
phenomenon.
Pre-rinsing consists of aspirating the first volume
of liquid and then dispensing it back into the same
vessel (or to waste). Subsequent volumes that you
pipette will have levels of accuracy and precision
within specifications.
This pre-rinsing operation should be repeated
when a new tip is used.

PIPETTING
8PIPETTING | PIPETMAN® FIXED
To adjust the volume setting to
compensate for a specific density
or viscosity, slide the key over
the push button until notches fit
into the grooves in the calibration
sleeve (Figure 4), and then turn
the key slowly:
●Clockwise to decrease the
volume.
●Counterclockwise to
increase the volume; go
above the desired value,
then decrease the volume
to reach the required
setting.
Example: When using a PIPETMAN F100 to
aspirate a particularly viscous solution, you
may determine gravimetrically that the volume
delivered is 98.8 µL, and the display reads
“E 0.2”. Therefore, you wish to increase the
volume dispensed by 1.2 µL. As the interval
between each letter on the calibration display
represents 0.4 µL for the F100 (1/10 of 4 µL), the
calibration tool must be turned three units until
the display reads “B 0.2”.
It is possible to alter the volume by more than
one full turn in the counterclockwise direction. In
this case, remember to readjust the same number
of turns when it is necessary to return to the
original setting.
Check the new volume gravimetrically. If the
volumes delivered are still not suciently close to
the desired value, make another slight adjustment
in the relevant direction.
Be sure to change tips between
volume setting adjustments and
to pre-rinse each new tip.
When the PIPETMAN Fixed is
readjusted to compensate for a
particular solution, the original
setting (ex. 0.2) can be noted on
the self-adhesive labels supplied
(Figure 5).
Figure 4
Volume Setting
Adjustment
Figure 5
Self-Adhesive
Labels
NOTE
Liquids of high density, vapor
pressure, or viscosity are best
pipetted by the Gilson MICROMAN®
positive displacement pipette.

ASSOCIATED TIPS
9
PIPETMAN® FIXED |USER’S GUIDE
PIPETTING | PIPETMAN® FIXED
Chapter 4
ASSOCIATED TIPS
PIPETMAN® DIAMOND Tips are made to the
highest specifications and strict quality control is
maintained throughout the manufacturing process.
These tips are used to calibrate PIPETMAN Fixed,
therefore for optimum performance we
recommend using PIPETMAN DIAMOND Tips with
your PIPETMAN Fixed.
To ensure accuracy and precision, Gilson's Quality
Assurance System focuses on the following critical
parameters:
●PIPETMAN DIAMOND Tips are made from
pure polypropylene (virgin, metal-free, to
avoid the possibility of contamination).
They are available sterilized and with filters.
●Optimized shape (revised collar for
optimum sealing, thin walls, and fine point),
making them easy to mount, flexible, with
no vortexing, and improved precision.
●PIPETMAN DIAMOND Tips are free from
even microscopic defects, especially
at the orifice. All surfaces are smooth
and hydrophobic, thereby avoiding the
excessive retention of liquids that causes
poor accuracy and a lack of precision.
●Mold and cavity
references are marked on
the collar, ensuring the
traceability. For quality
assurance purposes,
batch numbers appear on
all packages (bags and
boxes).
●They form an air-tight
seal with the tip holder,
preventing the leaks that
cause poor accuracy and
a lack of precision.
●PIPETMAN DIAMOND Tips (except filter
tips) may be autoclaved at 121°C for
20 minutes at 0.1 MPa.
NOTE
Liquids of high density, vapor
pressure, or viscosity are best
pipetted by the Gilson MICROMAN®
positive displacement pipette.
Figure 6
PIPETMAN
DIAMOND Tips
Traceability

ASSOCIATED TIPS
10 ASSOCIATED TIPS | PIPETMAN® FIXED
PIPETMAN DIAMOND Filter Tips
Filter tips are used when sample-to-sample,
sample-to-pipette, or sample-to-operator
contamination must be avoided.
Gilson's sterilized PIPETMAN DIAMOND Filter
Tips are certified free of detectable RNase,
DNase, DNA, RNA, and protease.
PIPETTE PIPETMAN DIAMOND TIPS TYPE
F2 to F100 D200 Regular
F200 D200/D300 Regular
F250 to F1000 D1000 Regular
F5000 D5000 Regular
PIPETTE PIPETMAN DIAMOND TIPS TYPE
F2 to F25 DF30ST Sterilized
F50 to F100 DF100ST Sterilized
F200 DF200ST/DF300ST Sterilized
F250 to F1000 DF1000ST Sterilized
To ensure the best performance
from your Gilson pipette, you
shoud ALWAYS use PIPETMAN
DIAMOND Tips (in accordance
with ISO 8655) to complete
your pipetting system, because
PIPETMAN DIAMOND Tips were
used to establish the specifications.
NOTE

RECOMMENDATIONS
11
PIPETMAN® FIXED |USER’S GUIDE
ASSOCIATED TIPS | PIPETMAN® FIXED
To ensure the best performance
from your Gilson pipette, you
shoud ALWAYS use PIPETMAN
DIAMOND Tips (in accordance
with ISO 8655) to complete
your pipetting system, because
PIPETMAN DIAMOND Tips were
used to establish the specifications.
Chapter 5
RECOMMENDATIONS
The recommendations below will ensure
maximum performance from your PIPETMAN
Fixed.
●Make sure to operate PIPETMAN Fixed
slowly and smoothly.
●When aspirating, keep the tip at a constant
depth below the surface of the liquid.
●PIPETMAN Fixed should be held in the
vertical position.
●Change the tip before aspirating a
different liquid, sample or reagent.
●Change the tip if a droplet remains on the
end of the tip from the previous pipetting
operation.
●Each new tip should be pre-rinsed with the
liquid to be pipetted.
●Liquid should never enter PIPETMAN Fixed
tip holder. To prevent this:
○Press and release the push button
slowly and smoothly.
○Never turn the pipette upside down.
○Never lay the pipette on its side
when there is liquid in the tip. Use a
dedicated stand or hanger: Gilson
CARROUSEL™, TRIO™, or SINGLE™.
●Never grease pistons or seals.
The pipette can be used
between 4°C and 40°C
but the specifications
may vary according to the
temperature (see CHAPTER 2 -
SPECIFICATIONS, on page 4, for
controlled conditions of use).
NOTE

TROUBLESHOOTING
12 TROUBLESHOOTING | PIPETMAN® FIXED
●When pipetting liquids with temperatures
different from the ambient temperature,
it is recommended to pre-rinse the tip
several times before use.
●Do not pipette liquids with temperatures
above 70°C or below 4°C.
●After pipetting acids or corrosive liquids
which emit vapors, it is recommended to
disassemble the tip holder and to rinse the
piston, the O-ring, and seal with distilled
water.
Chapter 6
TROUBLESHOOTING
The following table may help you to identify and
correct the problem you might encounter.
SYMPTOM POSSIBLE
CAUSE ACTION
PIpette won't
aspirate
Damaged tip
holder
Replace the tip holder.
For F2 to F20 models, if the tip
holder is damages, the piston may
also be damaged.Replace the
damaged parts. (See CHAPTER 7 -
SPARE PARTS,on page 14).
Connecting nut
is loose Tighten connecting nut
Damaged
piston and
seals
Replace the piston and seals
(see CHAPTER 7 - SPARE PARTS,
on page 14). Wash the inside of
the tip holder using distilled water.
Improper repair
or assembly
Disassemble the pipette and
reassemble the pipette correctly.
Refer to page 14.
Pipette is
inaccurate
Improper repair
or assembly
Disassemble the pipette and
reassemble the pipette correctly.
Refer to page 14.
Connecting nut
is loose Tighten connecting-nut
Tips fall o
or do not fit
correctly
Low quality tips Use PIPETMAN DIAMOND tips
Damaged tip
holder
Replace the tip holder. For F2 to
F20 models, if the tip holder is
damaged, the piston may also be
damaged. Replace the damaged
parts (See CHAPTER 7 - SPARE
PARTS, on page 14).
Damaged tip
ejector Replace tip ejector
The tip ejector
is loose Assemble the tip ejector properly

TROUBLESHOOTING
13
PIPETMAN® FIXED |USER’S GUIDE
TROUBLESHOOTING | PIPETMAN® FIXED
If there is liquid in the tip holder, clean the
pipette as follows:
●Remove the tip ejector. Unscrew the
connecting nut and rinse the tip holder,
piston, seal and O-ring with distilled water.
Dry these parts and reassemble the pipette.
If there is an air bubble when the sample is
aspirated:
●Dispense the sample into its original
vessel.
●Ensure that the tip is properly immersed in
the sample liquid.
●Aspirate the sample more slowly.
If the bubble appears a second time, replace the
tip.
The outside of the PIPETMAN Fixed can be
cleaned using a cloth dampened with isopropanol.
If a problem continues after carrying out the
above steps, contact your Gilson representative.
If necessary, the tip holder, connecting
nut and the tip ejector can be
autoclaved for 20 minutes at 121°C and
at 0.1 MPa pressure. The seal and the
O-ring should not be autoclaved.
WARNING
Before returning a pipette, please
ensure that it is completely free
of any chemical, biological,
or radioactive contamination.
Please contact your Gilson
Service Center to obtain the
decontamination form.

SPARE PARTS
14 SPARE PARTS | PIPETMAN® FIXED
Chapter 7
SPARE PARTS
B
C
D
E
F
PART NUMBER
ITEM F2 TO
F20
F25 TO
F100 F200 F250 TO F1000 F5000
Piston
assembly F123845 F144611 F123846 F123847 F123848
Seal &
O-ring
(5 sets) F144863 F144864 F144865 F144866 F144867
Connecting
Nut F144723 F144723 F144723 F144723 -
Tip ejector F144766 F144767 F144768 F144769 -
Service kit
1st level
(1 tip holder,
3 piston
seals and
3 O-rings) F144495 F144496 F144497 F144498 F144499
Adjustment
key F123674 F123674 F123674 F123674 F123674
Bags of 10
filters
Bags of 100
filters
- - - - F161280
- - - - F161241
A
B
C
D
E
F
A
NOTE
Contact your Gilson
representative to
order spare parts.

WARRANTY
15
PIPETMAN® FIXED |USER’S GUIDE
SPARE PARTS | PIPETMAN® FIXED
Chapter 8
WARRANTY
Gilson warrants this pipette against defects
in material under normal use and service
for a period of 12 months from the date of
purchase.
This warranty shall not apply to pipettes
which are subject to abnormal use and/
or improper or inadequate maintenance
(contrary to the recommendations given in
the user’s guide), including, but not limited
to pipettes which have been subjected
to physical damage, improper handling,
or spillage or exposure to any corrosive
environment. This warranty shall also be
void in the event pipettes are altered or
modified by any party other than Gilson or its
designates. Gilson’s sole liability under this
warranty shall be limited to, at Gilson’s sole
option, repair or replacement of any defective
components of pipettes or refund of the
purchase price paid for such pipettes.
THE FOREGOING WARRANTY IS EXCLUSIVE
AND GILSON HEREBY DISCLAIMS
ALL OTHER WARRANTIES, WHETHER
EXPRESS OR IMPLIED, INCLUDING ANY
WARRANTIES OF MERCHANTABILITY
AND ANY WARRANTIES OF FITNESS FOR
A PARTICULAR PURPOSE, UNDER NO
CIRCUMSTANCES SHALL GILSON BE LIABLE
FOR ANY CONSEQUENTIAL, PUNITIVE,
INDIRECT OR INCIDENTAL DAMAGES
ARISING OUT OF ANY BREACH OF ANY
EXPRESS OR IMPLIED WARRANTY.

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LT801118/N | ©08/2017 Gilson, Inc. All rights reserved.