Gilson Pipetman M User manual

User’s Guide
EN

TABLE OF CONTENTS
TABLE OF CONTENTS | PIPETMAN® M
TABLE OF CONTENTS
INTRODUCTION | 4
PARTS CHECKLIST AND ACCESSORIES | 5
Parts Checklist | 5
Accessories | 5
GETTING STARTED | 5
DESCRIPTION | 6
Reset | 6
Switch O | 6
Display | 6
PIPETTING JUST LIKE WITH PIPETMAN | 7
Switch On | 7
Adjust the Volume Setting | 7
Purge Volume | 7
PIPETTING MODES | 7
PIPET Mode | 7
REPETITIVE Mode | 8
MIX Mode | 9
REVERSE Mode | 11
CUSTOM Mode | 12
PIPETTING SPEED CONTROL | 13
PIPETMAN® DIAMOND TIPS | 13
GUIDELINES FOR GOOD PIPETTING | 15
Aspirate and Dispense | 15
Pre-Wet the Tips | 15
General Guidelines for Good Pipetting | 16
PERSONALIZE YOUR PIPETMAN® M | 16
Tip Ejector Extension for Use with P10M | 16
Personal Label – Name and/or Application Tag | 17
GOOD LABORATORY PRACTICE FEATURES | 17
General | 17
Cycle Counters | 17
Alarms | 18
POWER MANAGEMENT | 18
Battery Charging | 18
Working with Charger Connected to the Pipette | 19
Low Battery Warning | 19
Changing the Battery | 19

TABLE OF CONTENTS
PIPETMAN® M |USER’S GUIDE
CONFIGURATION | 20
MAINTENANCE | 20
Service Information | 21
Adjustment | 22
Standard Readjustment | 22
Maintenance Operation | 22
Maintenance Warning | 23
Maintenance Operation for Single Channel Models | 23
Maintenance Operation for Multichannel Models | 25
CLEANING AND DECONTAMINATION | 26
Cleaning | 26
Single channel Models | 26
Multichannel Models | 27
Decontamination | 27
Chemical decontamination – Single and Multichannel Models | 27
LEAK TEST | 28
TROUBLESHOOTING | 29
SPECIFICATIONS | 29
SAFETY PRECAUTIONS AND LIMITATIONS OF USE | 31
REPLACEMENT PARTS | 32
Single Channel Models | 32
Multichannel Models | 32
PIPETMAN M SOFTWARE | 33
What is PIPETMAN M Software? | 33
Passwords and User Information | 33
Home Screen | 34
Installing PIPETMAN M Software | 35
Uninstalling PIPETMAN M Software | 36
Creating and Managing Custom Protocols | 36
Transferring Custom Protocols to the Pipette | 42
Updating Pipette Settings | 44
Updating Pipette Firmware | 45
Other Administration | 46
EC DECLARATION OF CONFORMITY | 49
WARRANTY | 50
APPENDICES | 51
Appendix A – Example of a Performance Check | 51
Appendix B – Z Factor | 52
Appendix C – Evaporation Loss | 53

INTRODUCTION
4INTRODUCTION | PIPETMAN® M
Chapter 1
INTRODUCTION
Congratulations on the purchase of your PIPETMAN® M, the electronic pipette with
guaranteed performance in both standard and repetitive pipetting modes.
PIPETMAN M is a motorized member of the PIPETMAN® family of pipettes from Gilson,
requiring virtually no pipetting forces to aspirate and dispense samples. Combining
PIPETMAN’s renowned accuracy, precision, and robustness with user-friendly
functions, PIPETMAN M reduces pipetting fatigue, and increase pipetting efficiency.
PIPETMAN M is a unique pipette with only two buttons to operate the pipette and
reach all menu options:
●Push button: set the volume by turning the button left or right and then
pipette with a simple push.
●Mode button: access additional features like repetitive mode, mix, or
maintenance functions.
Only two buttons are needed to perform routine pipetting tasks.
PIPETMAN M features the classic PIPETMAN® look with additional functionalities:
●Intuitive interface with five pipetting modes for a large number of applications:
standard pipetting, repetitive, mix, reverse, and custom mode.
●Minimal effort: aspirate and dispense with one click of
the push button and eject tips with ease to help reduce
the risk of repetitive strain injuries (RSI).
●Maximum comfort: the lightweight and balanced design
of PIPETMAN M offers an ergonomic design that rests
comfortably in your hand as you pipette.
●Minimized pipetting variability: the motorized piston
allows for high reproducibility and accuracy while
pipetting.
●Increased performance for all day pipetting comfort.
PIPETMAN M is available in 20 models covering
a range from 0.5 µL to 10 mL in single channel and
0.5 µL to 1200 µL in multichannel:
Mode
button
Push
button
PIPETMAN M MULTICHANNEL
Model Part Number Volume Range
P8x10M F81025
0.5–10 µL
P12x10M F81026
P8x20M F81027
1–20 µL
P12x20M F81028
P8x100M F81018
10–100 µL
P12x100M F81019
P8x200M F81029
20–200 µL
P12x200M F81030
P8x300M F81031
10–300 µL
P12x300M F81032
P8x1200M F81020
50–1200 µL
P12x1200M F81021
PIPETMAN M SINGLE CHANNEL
Model Part Number Volume Range
P10M F81022 0.5–10 µL
P20M F81023 2–20 µL
P100M F81013 5–100 µL
P200M F81024 20–200 µL
P300M F81014 20–300 µL
P1200M F81015 100–1200 µL
P5000M F81016 500–5000 µL
P10mLM F81017 1–10 mL
Chapter 1

PARTS CHECKLIST AND ACCESSORIES
5
PIPETMAN® M |USER’S GUIDE
Chapter 2
PARTS CHECKLIST AND ACCESSORIES
Parts Checklist
Take a moment to verify that the following items are in the box:
●PIPETMAN M pipette
●Power Supply 5V with AC adapter and cable
●4 Battery side tags
●4 Ejector side tags
●Tip ejector extension (P10M model only)
●Quick Guide
Accessories
ACCESSORIES PART NUMBER
POWER CARROUSEL: 5–position charging carrousel for single and
multichannel models FB1001
Battery side tags (set of 4) F807013
Ejector side tags (set of 4) F807014
Chapter 3
GETTING STARTED
Please contact your Gilson representative to order additionnal accessories.
NOTE
PIPETMAN M is provided with a minimal battery charge. Before using your
new PIPETMAN M, we strongly recommend that you to fully charge the
battery. PIPETMAN M charges 80% of its full battery capacity in less than an
hour, it takes three hours to fully charge the battery. Please refer to
Chapter 12 Power Management on page 18 for charging procedure.
Switch on your PIPETMAN M. Press the push button to activate
the pipette. The start-up screen appears:
The pipette will do a self calibration test and the firmware
will reset the piston.
During initialization, the volume range, version of the
firmware, and the number of readjustments is displayed on
the screen (refer to Standard Readjustment on page 22).
Your PIPETMAN M is preset to PIPET mode
and is now ready to pipette (Refer to Chapter 5
Pipetting Just Like with PIPETMAN on page 7).
PIPETMAN M goes into sleep mode (display turns off) after three minutes of inactivity.
Press the push button to reactivate the pipette. Your last setting and pipetting mode
will appear on the screen.
●Safety bag
●Certificate of conformity
(including bar-code sticker)
●Lubricant (except for P10M,
and the multichannel models)
Volume
range
Version of
firmware
Number of
readjustments

DESCRIPTION
6DESCRIPTION | PIPETMAN® M
Figure 1
PIPETMAN® M single and multichannel models
Chapter 4
DESCRIPTION
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18 19
Upper part
Push button and volume
adjustment knob
Mode button: direct access to all
menu functions
Tip ejector button
Display
Elastomer plug to cover the battery
charging port
Identity-tag clip
Identity-tag window
Connecting nut — attaches handle
to lower part
Lower part
Tip ejector: removable to access tip
holder
Optimized tip holder to reduce tip
fitting and ejection forces, removable
for cleaning and servicing
Ejector clip
Ejector support
Cover
Ejector spacer
Screen
Pipetting Mode
Aspirate and dispense indicator
Battery status
Purge indicator
Volume
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
Reset
To reset your pipette, simultaneously press on the push button and mode button
and hold for at least five seconds.
Switch o
To switch off your PIPETMAN M, press the push button and hold for at least five
seconds.
Display
The PIPETMAN M display is an Organic Light-Emitting Diode (OLED) screen. It shows
the current mode and operation step, pipetting volume, battery indicator and piston
status (aspirated and dispensed volume) in real time.Warning messages appear in the
place of volume.
12
1

PIPETTING JUST LIKE WITH PIPETMAN
7
PIPETMAN® M |USER’S GUIDE
Chapter 6
PIPETTING MODES
PIPETMAN M offers more pipette modes for a large number of applications: you
will find all pipetting modes in the menu (for system settings refer to the Chapter 7
Pipetting Speed Control on page 13 and Chapter 13 Configuration on page 20). Access
the menu by pushing the mode button. To choose one of the pipetting modes, turn
the push button and then click to confirm your selection.
PIPET Mode
This is the classic pipette mode (forward pipetting) for simple
aspirating and dispensing.
•
Chapter 6
Chapter 5
PIPETTING JUST LIKE WITH PIPETMAN
Using PIPETMAN M is as easy as using a mechanical PIPETMAN.
Your PIPETMAN M is set by default to “PIPET Mode”, which means
forward pipetting. Select a volume and then start to pipette.
Switch On
Press push button.
Adjust the Volume Setting
1. Hold your PIPETMAN M in a nearly vertical position.
2. Turn the push button half way. The screen will display:
“ Click to change volume. ”
3. Press the push button; the volume on the display will start blinking and
you can now adjust the volume:
●Turn the push button either clockwise to decrease volume or counter
clockwise to increase volume, as indicated on the button.
●Press the push button one time when finished. The volume is locked.
Purge Volume
The tip can be emptied at any time during a pipetting cycle.
1. Turn the push button quickly: the message “Click to abort” will appear on the
screen.
2. Click on the push button to validate: the liquid will be dispensed and an automatic
purge followed by piston reset to zero will occur.
NOTE
You can use the PIPET Mode for all standard applications like DNA
extraction, plasmid isolation, cloning, dilution, PCR, qPCR and many others.
You can easily pipette aqueous liquids like buer, chemical solutions
(MgCl2, KCl …), and biological samples such as blood, DNA, and RNA.
Chapter 5
Figure 1
PIPETMAN® M single and multichannel models

PIPETTING MODES
8DESCRIPTION | PIPETMAN® M
1. Fit a tip suitable for the model of PIPETMAN M that you are using (preferably use
PIPETMAN DIAMOND Tips for assurance of accurate and precise results, refer to
Chapter 8 PIPETMAN DIAMOND Tips on page 13).
2. Press the push button to aspirate the selected volume.
3. To dispense: click (press and release) the push button.
Three things happen: sample dispense, automatic
purge, and piston reset to zero. Or, keep your thumb
pressing the push button until the end of the dispense
cycle: the piston reset takes place after releasing the
push button again. This feature enables you to remove
the tip from the liquid, without aspirating anything.
4. Eject tip by pressing the ejection button.
Your PIPETMAN M is ready for the next pipetting cycle while
maintaining the volume and the last settings.
Continue pipetting as you would with your mechanical PIPETMAN; it’s as simple as
that to use your new, electronic pipette!
REPETITIVE Mode
The REPETITIVE Mode allows you to distribute the same volume repeatedly in a
predefined number (N) of equal aliquots. You supply the aliquot volume
(AV) and the pipette automatically calculates the number of aliquots
possible from the nominal (maximum) volume (NV) of the pipette as
follows:
N = NV/AV (e.g., 120 μL x 10 for P1200M).
You also can decrease the number of repetitions (e.g., 120 μL x 10-(n ≥1) for P1200M).
NOTICE
Always fit a tip before using any pipette.
START button
Optional STOP
0
V
Purge
Figure 2
PIPET Mode
NOTE
The REPETITIVE Mode is ideal for dispensing aliquots, e.g., dispensing
a PCR master mix into PCR tubes or 96–well, pipetting elution buer for
DNA extraction, preparing a target for spectrometric analyses, distributing
loading buer into samples, etc.
1. Press mode button to access system menu, where you can choose between
different pipette modes. Select REPETITIVE Mode by turning the push button.
To confirm your selection click on the push button.
2. Turn the push button half way, the screen will display: “Click to
change volume.”
3. Press the push button; the volume on the display will start to blink, and you can
now adjust the volume.
Set the aliquot volume
1. Click the push button, set the volume by turning the push button and click again
to confirm your settings.
2. Set the aliquot number: the maximal number of aliquots has been calculated by
your PIPETMAN M. The number of aliquots flashes and you can decrease this
number (N – (n ≥1)) by turning the push button.
3. Press again to confirm your settings.

PIPETTING MODES
9
PIPETMAN® M |USER’S GUIDE
4. Fit a tip suitable for the model
of PIPETMAN M that you
are using (preferably use
PIPETMAN DIAMOND Tips
for assurance of accurate and
precise results; refer to Chapter8
PIPETMAN DIAMOND Tipson
page 13).
5. Press push button to aspirate
total volume. The volume
aspirated will be a little bit more
than required (extra volume).
The extra volume is necessary to
ensure equal operating conditions
for each dispensed aliquot.
Dispense
Press the push button. DISCARD appears on the screen. Discard the extra volume
andcontinue dispensing the aliquots. PIPETMAN M distributes the aliquot volume each
time you click the push button. The number of aliquots left to dispense is displayed on
the screen. A warning beep indicates the last aliquot.
Purge
Press the push button, PURGE appears on the screen, click again to purge and reset
the piston. Keep your thumb pressing the push button until the end of the purge: the
piston reset takes place after you release the push button again. This feature enables
you to remove the tip from the liquid, without aspirating anything.
Eject Tip
Pressing the ejection button.
MIX Mode
This is the classic pipette mode (forward pipetting) followed by a mixing phase, composed
of repeatedly aspirating and dispensing, as well as an optional forward pipetting step.
1. Press mode button to access menu, where you can choose between different pipette
modes. Select MIX Mode by turning the push button, to confirm your selection click
on the push button.
2. Set the first volume, named VOLUME 1: press the push button, set the volume by
turning the push button and click to confirm your selection. This is the first volume
you will aspirate and dispense. Set the second volume, named VOLUME 2: press the
push button, you can now set the second volume by turning the push button.
Click again to confirm your settings.
NOTICE
Always fit a tip
before using
any pipette.
START button Optional STOP
V (+ extra vol)*
Purge
Start Purge
V (vol)
0
DISCARD ALIQUOTS PURGE
Click to Purge
Discard
Figure 3
REPETITIVE Mode
NOTE
The MIX Mode can be used to prepare a PCR master mix, enzyme restriction
mix, protein solution, oligonucleotide dilution and to mix samples with
gel loading buffer and so on…you can mix two different solutions or
homogenize one solution. If you work with higher viscosity than water (e.g.,
restriction enzyme), you may change the aspiration speed (refer to Chapter 7
Pipetting Speed Control on page 13). If you work with genomic DNA, then you
should always pipette very carefully to avoid shearing and nicking.

PIPETTING MODES
10 DESCRIPTION | PIPETMAN® M
3. Set the MIX volume: press the push button, set the volume by turning the push
button. Confirm your settings with a simple click. This is the volume which will
be aspirated and dispensed repeatedly. “MIX” as long as you press the push
button.
4. Set the additional pipetting volume, named VOLUME 3: You can choose a
volume to be aspirated and dispensed after the mixing step, so you can continue
routine pipetting.
Press the push button, and then set the volume by turning the push button.
Confirm your settings by a simple click.
5. Fit a tip suitable for the model of PIPETMAN M that you are using (preferably
use PIPETMAN DIAMOND Tips for assurance of accurate and precise results;
refer to Chapter8 PIPETMAN DIAMOND Tips on page 13).
Aspirate VOLUME 1
Press the push button.
Dispense
Press and release the push button:
three things happen: sample
dispense, automatic purge and piston
reset to zero. Or, keep your thumb
pressing the push button until the
end of the dispense cycle: the piston
reset takes place after you release the
push button again.
This feature enables you to remove
the tip from the liquid, without
aspirating anything.
Aspirate and Dispense the
Second Volume
“VOLUME 2”, repeat steps Aspirate Volume 1 and To dispense.
Mix
Press push button; as long as you keep your thumb pressing the button, the pipette
continues mixing. Release the push button to complete the current mixing cycle.
Purge
Press push button, PURGE appears on the screen, click again to purge and reset the
piston.
Pipette
If you have chosen a VOLUME 3 > 0, you can now aspirate and dispense this volume
by repeating steps 2 and 3.
NOTE
If you set VOLUME 1 or VOLUME 2 = 0 µL, then the step will not appear.
If you set VOLUME 1 and 2 = 0 µL, then the cycle will start directly with
the MIX step.
If you set the VOLUME 3 = 0 µL, then the cycle will stop after the MIX
step.
NOTICE
Always fit a tip before using any pipette.
NOTE
NOTE
If you choose VOLUME 1 = 0, then
step 2 won’t occur, if you choose
VOLUME 2 = 0 µL, then step 4 won’t
occur, as well if you set VOLUME
1 and 2 = 0 µL, then step 1–4 will
disappear. If you set VOLUME 3 =
0 µL step 7, then will not occur.
Figure 4
MIX Mode
START button Optional STOP
0
V
Purge
Start Purge
Push button pressed
V1 V2 V3

PIPETTING MODES
11
PIPETMAN® M |USER’S GUIDE
REVERSE Mode
The REVERSE Mode is reverse pipetting with a manual pipette. During
aspiration, additional liquid is added. After delivery, the excess volume
remains in the tip and is discarded.
The REVERSE Mode is ideal for pipetting viscous liquids; liquids with high vapor
pressure or those that tend to foam. Your PIPETMAN M aspirates a selected volume
and an excess. This excess compensates for the liquid that remains as a film inside the
tip during dispensing. For example: protein extraction, cell lysis, plasmid isolation, cell
culture, buffer preparation and, many others.
Electrophoresis Gels Loading Protocol
Electrophoresis gels loading protocol: first change the pipetting speed of your pipette
(refer to Chapter 7 Pipetting Speed Control on page 13). Aspiration can be done by
using the standard speed (by default speed 6). Dispensing should be done very slowly
and carefully, to prevent swirling and spilling of the samples, so select the lowest speed 1.
1. Press mode button to access system menu, where you can choose between
different pipette modes. Select REVERSE Mode by turning the push button.
To confirm your selection click, the push button.
Set the volume
1. Press the push button, set the volume by turning the push button and press again
to confirm your settings.
2. Fit a tip suitable for the model of PIPETMAN M that you are using (preferably use
PIPETMAN DIAMOND Tips for assurance of accurate and precise results; refer to
Chapter 8 PIPETMAN DIAMOND Tips on page 13).
Aspirate
Press push button to aspirate the selected volume.
An amount of liquid equal to the amount of purged
air is added. The volume aspirated will be a little bit
more than the set volume.
Dispense
Press push button to dispense the volume, the
additional amount of liquid remains in the tip.
Purge
Press push button, ‘‘PURGE’’ appears on the
screen, press again to purge and reset the piston.
Keep your thumb pressing the push button until
the end of the purge: the piston reset takes place
after you release the push button again.
This feature enables you to remove the tip from
the liquid, without aspirating anything.
Eject tip
Press the ejection button.
NOTICE
Always fit a tip before using any pipette.
0
Purge
START button
Optional STOP Start Purge
V (+ extra vol)*
Click to Purge
Figure 5
REVERSE Mode
NOTICE
If you purge without pulling out the tip air bubbles could get into your gel!

PIPETTING MODES
12 DESCRIPTION | PIPETMAN® M
CUSTOM Mode
The CUSTOM Mode enables personalized pipetting protocol creation on a PC for
transfer to PIPETMAN M through PIPETMAN M programmable software, an easy-to-use
software. To use this mode, the software has to be installed on the PC (file downloadable
on www.gilson.com/pipetmanm).
To install PIPETMAN M software:
1. Download PIPETMAN M software zip file and extract the installation files.
Then choose the programm corresponding to your operating system (Windows®
32-bit or 64-bit).
2. Double-click the installer and follow the instructions on the screen to complete the
installation.
3. When completed, the PIPETMAN M Software icon will appear on desktop of the PC if
“ Create desktop shortcut ” was selected.
4. If PIPETMAN M Software is not launched automatically, then double-click the
PIPETMAN M icon on the desktop.
Once installed, the PIPETMAN M Software can be run by any user on the installed PC.
You can then create, edit, import, or export custom protocols easily and rapidly.
To create a protocol, choose tasks in PIPETMAN M programmable software:
●Aspirate: aspirates specified volume into the tip.
●Beep: makes pipette produce a beep sound while selected tasks are executed.
●Dispense: delivers the specified volume from the tip.
●Dispense all: dispenses all remaining volume from the tip followed by a purge
action.
●Mix: aspirates then dispenses a specified volume one or more times.
●Purge: dispenses all remaining volume from the tip followed by purging extra
volume.
●Repetitive: dispense liquid into a number of aliquot.
●Reverse: handle liquid using reverse pipetting.
●Wait: introduces a wait for a specified number of seconds.
●Wait for click: pauses the protocol until Push button is clicked.
In this mode, the protocols can be automatically executed, avoiding repetitive pushes on
the button, helping to decrease risk of RSI.
To transfer a protocol to your PIPETMAN M pipette, click Transfer custom protocols to
pipette and follow the instructions on the screen.
The CUSTOM Mode is available in pipettes from firmware version V2.02.
For more information on PIPETMAN M Software, refer to Appendix B - Z Factor on page 52.

PIPETTING SPEED CONTROL
13
PIPETMAN® M |USER’S GUIDE
Chapter 7
PIPETTING SPEED CONTROL
PIPETMAN M is set by default on speed 6 (maximum speed). You may need to change
the speed of aspiration or dispensing depending on your application. You can change
aspiration speed and dispensing speed independently of one other: from very slow to
very fast (speed 1 — speed 6). Your speed selection is memorized for each pipetting
mode until you change it again.
1. Press mode button to access system menu, where you can find the different
pipette modes, the speed menu and configuration menu.
2. Select SPEED by turning the push button, to confirm your selection click on
the push button.
3. Set ASPIRATION SPEED: aspiration speed is blinking, set the speed by turning the
push button and click to confirm your selection.
4. Set DISPENSING SPEED: dispensing speed is blinking, set the speed by turning
the push button and click to confirm your selection.
After selecting the speed you go automatically back to pipetting mode.
Chapter 8
PIPETMAN® DIAMOND TIPS
PIPETMAN DIAMOND Tips are made to the highest specifications;
strict quality control is maintained throughout the manufacturing
process. These tips are used to calibrate PIPETMAN M, therefore
for optimum performance we recommend using PIPETMAN Tips
with your PIPETMAN M.
However, PIPETMAN M also offers you a high compatibility with a
large number of other tips. For more information, please contact
your Gilson distributor.
Every PIPETMAN DIAMOND Tip is individually marked with an
identification number (ID). To ensure accuracy and precision,
Gilson’s quality assurance system focuses on the following critical
parameters:
●PIPETMAN DIAMOND Tips are made from pure polypropylene (virgin, metal-free,
to avoid the possibility of contamination). They are available sterilized and with
filters.
●Sterilized PIPETMAN DIAMOND Tips are certified free of detectable RNases,
DNases, DNA, RNA, and proteases.
●Optimized shape (revised collar for optimum sealing, thin walls, and fine point),
making them easier to mount, more flexible, with no vortexing, and improved
precision.
●PIPETMAN DIAMOND Tips are free from even microscopic defects, especially
at the orifice. All surfaces are smooth and hydrophobic, thereby avoiding the
excessive retention of liquids that causes poor accuracy and a lack of precision.
ID Number
Figure 6
ID number

PIPETMAN® DIAMOND TIPS
14 PIPETMAN® DIAMOND TIPS | PIPETMAN® M
●Mold and cavity references are marked on the collar, ensuring the traceability. For
quality assurance purposes, batch numbers appear on all packages (bags and boxes).
●They form an air-tight seal with the tip holder, preventing the leaks that cause
poor accuracy and a lack of precision.
●PIPETMAN DIAMOND Tips (except filter tips) may be autoclaved at 121°C for
20 minutes at 0.1 MPa.
NOTICE
Do not autoclave PIPETMAN Filter Tips as the filter will be damaged.
NOTE
To ensure the best performance from your Gilson pipette, you
shoud ALWAYS use PIPETMAN DIAMOND Tips (in accordance with
ISO8655) system, because PIPETMAN Tips were used to establish the
specifications.
PIPETMAN M MULTICHANNEL
P8x10M D10
DL10
DF10
DFL10 0.5–10 µL
P12x10M
P8x20M DL10
D200
DFL10
DF30 1–20 µL
P12x20M
P8x100M
D200 DF100 10–100 µL
P12x100M
P8x200M D200
D300
DF100
DF200
DF300
20–200 µL
P12x200M
P8x300M D200
D300
DF200
DF300 10–300 µL
P12x300M
P8x1200M
D1200 DF1200 50–1200 µL
P12x1200M
PIPETMAN M SINGLE CHANNEL
VARIABLE VOLUME MODELS
Model PIPETMAN
DIAMOND Tips
Volume Range
P10M D10*
DL10*
DF10
DFL10 0.5–10 µL
P20M D200 DF30 2–20 µL
P100M D200 D100 5–100 µL
P200M D200
D300
DF200
DF300 20–200 µL
P300M D200
D300
DF200
DF300 20–300 µL
P1200M D1000
D1200
DF1000
DF1200 100–1200 µL
P5000M D5000 500–5000 µL
P10mLM D10mL 1–10 mL
Figure 7
Plastic adapter
Figure 8
P8x10M and P12x10M
Long tips Short tips
Ejector spacer Ejector spacer D10
* A plastic adapter is required to eject D10 and
DF10 tips (short tips). The adapter is supplied
with P10M. No adapter is required when using
DL10 and DFL10 tips (long tips).

GUIDELINES FOR GOOD PIPETTING
15
PIPETMAN® M |USER’S GUIDE
Chapter 9
GUIDELINES FOR GOOD PIPETTING
Aspirate and Dispense
Fit new PIPETMAN DIAMOND Tips for the best results (refer
to Chapter 18 Specifications on page 29).
Single Channel Model
For single channel models, push the tip holder into the tip
using a slight twisting motion to ensure a firm and airtight seal.
MultiChannel Model
PIPETMAN DIAMOND tips are best fitted with the
ROCKY RACK technique, invented by Gilson, available only
in our TIPACK and TOWERPACK.
Pre-Wet the Tips
All Models
Pre-wetting the tips before pipetting helps prepare the tips for the best
pipetting performance. Ideally, the pre-wet includes both immersing the tip
in the liquid and performing one pipetting step.
Pre-wetting the tips helps ensure
that volumes that you pipette will
achieve accuracy and precision within
specifications.
Hold the pipette vertically and immerse
the tip in the liquid (refer to page 14).
Press the push button to aspirate the set
volume of liquid. Wait a few seconds (time
depends on model, refer to page 14); then
withdraw the pipette tip from the liquid.
You may wipe any droplets away from
the outside of the tip using a medical
wipe; however if you do so, take care to
avoid touching the orifice of the tip.
Place the end of the tip against the inside
wall of the recipient vessel (at an angle of
10° to 40°). Press the push button. Wait
for at least a few seconds before releasing
the push button to expel any residual
liquid from the tip. While removing the
pipette draw the tip along the inside
surface of the vessel.
Eject the tip by pressing firmly on the tip
ejector button.
MODEL IMMERSION
DEPTH (MM)
WAIT TIME
(SECONDS)
P10M 1 1
P20M 2–31
P100M 2–41
P200M 2–4 1
P300M 2–41
P1200M 2–42–3
P5000M 3–64–5
P10mLM 5–74–5
P8x10M/P12x10M 1 1
P8x20M/P12x20M 2–31
P8x100M/P12x100M 2–41
P8x200M/P12x200M 2–31
P8x300M/P12x300M 2–31
P8x1200M/P12x1200M 2–42–3
Table 1
Immersion depth and wait time
Chapter 9

PERSONALIZE YOUR PIPETMAN® M
16 PERSONALIZE YOUR PIPETMAN® M | PIPETMAN® M
General Guidelines for Good Pipetting
●Make sure that you fit new tips.
●Each new tip should be pre-wetted with the liquid to be pipetted.
●When aspirating, keep the tip at a constant depth below the surface of the liquid
(refer to table 1 on page 15).
●Change the tip before aspirating a different liquid, sample, or reagent.
●Change the tip if a droplet remains at the end of the tip from the previous pipetting
operation.
●Liquid should never enter the tip holder. To prevent this:
○Never turn the pipette upside down,
○Never lay the pipette on its side when there is liquid in the tip(s),
The Gilson Stand Adapter (refer to Chapter 2 Parts Checklist and Accessories on page 5)
is recommended for use with the CARROUSELTM and SINGLETM Pipette Holder to store
your PIPETMAN M pipette in the vertical position. Alternatively the POWER CARROUSELTM
can be used to store and charge up to five PIPETMAN M pipettes.
●When pipetting liquids with temperatures different to the ambient temperature,
pre-we the tip several times before use in order to reach equilibrium between the
temperatures of the liquid and the pipette’s dead volume.
●For volatile liquids, saturate the dead volume by aspirating and dispensing the liquid
repeatedly before aspirating the sample.
After pipetting acids or other corrosive liquids that emit vapors, clean the pipette, as
described in Chapter 15 Cleaning and Decontamination on page 26.
The pipette can be used between 4°C and 40°C, but the specifications may vary (refer to
Chapter 18 Specifications on page 29).
●Do not pipette liquids at temperatures above 50°C or below 4°C.
Chapter 10
PERSONALIZE YOUR PIPETMAN® M
Tip Ejector Extension for Use with P10M
Tip ejector extensions is required to eject D10 tips and is supplied with P10M pipettes.
The tip ejector extension which is made of PVDF
(polyvinylidene Fluoride), is autoclavable.
To fit a tip ejector extension:
1. Hold the pipette with the push button upright.
2. Hold the extension with the slot upright.
3. Slide the extension over the tip holder.
4. Push the extension firmly onto the end of the tip
ejector until it clicks into place (see opposite). To fit To remove
Slot
NOTE
Extreme temperatures can aect accuracy and precision.
Figure 9
Fit a tip ejector

GOOD LABORATORY PRACTICE FEATURES
17
PIPETMAN® M |USER’S GUIDE
To remove a tip ejector extension (see opposite):
1. Hold the pipette in one hand and grip the extension with the other.
2. Gently twist the extension (either direction) and pull it away
from the pipette.
Personal Label – Name and/or
Application Tag
Your PIPETMAN M offers you two options to mark your pipette.
You can personalize your pipette with a name tag and/or an application tag:
Window Tag:
1. Remove the window by inserting a small
screwdriver in the access slot.
2. Position the name tag on the pipette.
3. Clip the window back in place.
Clip Tag:
1. Pull off the clip.
2. Position the name tag into the clip.
3. Clamp the clip back in place.
Short
tips Long
tips
Window tag
Clip tag
Chapter 11
GOOD LABORATORY PRACTICE FEATURES
Your PIPETMAN M is fully compliant to ISO8655 standard and is CE marked (for EMC
and LDV directives).
PIPETMAN M includes the following good laboratory practice (GLP) features.
General
●Lockable volume.
●Volume range is printed on the push button.
●Volume range is displayed on the screen.
●Serial number is engraved on the body (handle) of the pipette and encoded in the
firmware.
●Bar code: on the box and with the certificate (can be transferred).
Personalization by nametags (for marking application and/or user name), refer to
Chapter 10 Personalize your PIPETMAN M on page 16.
Cycle Counters
●From last volume setting (to count the number of cycles in the current “run”).
●From manufacture and since the last service (refer to
Chapter 14 Maintenance on page 20).
●By indicators (R1, R2, R3 … Rx) each time pipette is readjusted.
Maintenance intervals can be specified by weeks or number of cycles elapsed.
Figure 10
Remove a tip ejector
Figure 11
Name and application tag

POWER MANAGEMENT
18 POWER MANAGEMENT | PIPETMAN® M
Alarms
●Low battery warning.
●Service is overdue warning (refer to Chapter 14 Maintenance on page 20).
●Service alert can be set “On” or “Off”.
Chapter 12
POWER MANAGEMENT
The PIPETMAN M battery has been
designed to ensure 900 pipetting cycles
(750 for PIPETMAN M Multi 200 µL and
300 µL) at maximum speed (up to nine
96-well plates) between two charge cycles.
For service continuity when needing more
capacity, your PIPETMAN M can also
be used while charging with the same
performance as a fully charged pipette.
Battery Charging
PIPETMAN M charges 80% of its full battery
capacity in less than an hour, and it takes three
hours to fully charge the battery.
Charge your pipette by using the power supply:
1. Connect the AC adapter to a suitable
AC power supply.
2. Plug the adapter into the USB port
of the pipette.
The pipette starts charging.
Charge your pipette by using stand adapter (refer
to Chapter 2 Parts Checklist and Accessories on
page 5):
1. Place the stand adapter on your Gilson Single
Pipette Holder or Carrousel.
2. Remove the plug. Now place your pipette on the
Stand Adapter and plug the power transformer
into the stand adapter.
The pipette starts charging.
Battery charging of PIPETMAN M using the
POWER CARROUSEL:
1. Install the POWER CARROUSEL as described
above.
2. Remove the plug. Now place your pipette on the
charging position, please ensure
that the pipette is properly fitted to the contacts
on the top of the carrousel (a proper fitting will
trigger a beep sound).
The pipette starts charging.
Stand adapter
Plug
USB port
Figure 12
PIPETMAN® M Power supply
Figure 13
POWER CARROUSEL for PIPETMAN® M

POWER MANAGEMENT
19
PIPETMAN® M |USER’S GUIDE
Working with Charger Connected to the Pipette
PIPETMAN M can also be used for pipetting while charging.
1. Plug the power supply into your pipette.
2. Press the push button to start your pipette.
PIPETMAN M is ready to pipette.
Low Battery Warning
PIPETMAN M has a low battery alert. As the battery runs down, the
low battery message appears. The warning is replaced by a blinking
symbol of an empty battery. If it is not recharged, the pipette will switch off after a
while. Settings will not be lost.
If the battery is completely discharged (black screen, no operation) connect the
pipette to the charger for at least five minutes, and then a “batt. too low” message
will appear. The pipette will display the last settings used a few minutes later and will
continue charging.
Changing the Battery
If the following symptoms occur, please contact your Gilson service center:
●PIPETMAN M battery won’t charge or will not operate even if connected to the
charger at least for 10 minutes.
●PIPETMAN M battery provides you very short cycle time or permanently needs
to be used in connected mode.
NOTE You can also recharge PIPETMAN M by USB with your PC; however it will
take much longer than by power supply.
NOTICE
Use only the original power transformer supplied by Gilson. Use of an
incompatible power supply can damage your PIPETMAN M.
Do not operate PIPETMAN while connected to a PC.
CAUTION PIPETMAN M uses a Lithium-Ion battery. Operation on the battery is done
at user’s risk only. Opening the pipette voids the warranty.

CONFIGURATION
20 CONFIGURATION | PIPETMAN® M
Chapter 13
CONFIGURATION
Enter the menu using the mode button. You will find not
only the pipetting programs and the speed control, but
also the menu CONFIGURATION, including SERVICE and
ADJUSTMENT (refer to Chapter 14 Maintenance below).
The navigation of the menus is always done in the same
way — make your selection by rotating the push button and
then enter and confirm with a click.
The Configuration menu allows you to set up the following items:
●Volume limit: Here you can fix the maximal volume of the
pipette (by default = nominal volume). For example, you
have a P200M pipette, but you would like to use it with
your PIPETMAN Diamond Filter Tips DF100, you can set
the maximal
volume at 100 µL and you won’t risk contamination of your pipette.
●Beeper: You can switch the tone of your pipette on or off.
●Contrast: You can choose a value of 1 to 5 to set up the contrast of the screen.
●Service: refer to Chapter 14 Maintenance below
Adjustment: refer to Chapter 14 Maintenance below.
●Language: Available from the firmware version 1.20.
Languages available are English, Chinese and Japanese.
To change the language, enter in the Configuration menu:
1. Select your language by turning the push button.
2. Click on the push button to confirm your selection.
The language selected is blinking.
3. Press and hold the Mode button for two seconds to confirm your selection.
Mode button
Push button
Chapter 14
MAINTENANCE
PIPETMAN M requires very little maintenance. However, to ensure pipette accuracy,
precision, and robustness please proceed periodically with a two–minute inspection as
recommended by Gilson.
Your PIPETMAN M allows you to:
●Get service information.
●Readjust the pipette to user settings.
●Return to factory settings.