Hach DR2400 User manual

59400-18
Hach
Model DR/2400
Portable
Spectrophotometer
© Hach Company, 2004. All rights reserved. Printed in the U.S.A. te/dk 05/04 3ed

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Table of Contents
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Table of Contents
Safety Information.....................................................................................................................................................7
Specifications .............................................................................................................................................................9
Installation........................................................................................................................................................... 11
Section 1 Getting Started ...........................................................................................................................13
1.1 Unpacking the Instrument............................................................................................................................13
1.2 Operating Environment ................................................................................................................................13
1.3 Cable Connections .........................................................................................................................................13
1.3.1 Power Connections ...............................................................................................................................14
1.3.2 Serial Printer and PC Connections .....................................................................................................14
1.4 Battery Pack ....................................................................................................................................................14
1.5 Using the Instrument Keys...........................................................................................................................15
1.5.1 Turning the Power On and Off ...........................................................................................................15
1.5.2 Turning On the Backlight.....................................................................................................................16
1.5.3 Adjusting the Display Contrast ..........................................................................................................16
1.5.4 Battery Status.........................................................................................................................................16
1.6 Tips for Using the Touch Screen ..................................................................................................................17
1.7 The DR/2400 Portable Spectrophotometer Main Menu ..........................................................................17
1.8 Using the DR/Check™ Absorbance Standards ........................................................................................18
1.8.1 How to Use the Standards ...................................................................................................................18
1.9 Determining Photometric Accuracy............................................................................................................19
Section 2 General Instrument Operation...........................................................................................21
2.1 Selecting a Language .....................................................................................................................................21
2.2 Instrument Setup Menu ................................................................................................................................21
2.2.1 Setting the Time and Date....................................................................................................................21
2.2.2 Setting Sound Preferences....................................................................................................................21
2.3 Using the Alphanumeric Keypad................................................................................................................22
2.3.1 Setting the Operator ID ........................................................................................................................22
2.3.2 Setting the Sample ID ...........................................................................................................................23
2.3.3 Right- or Left-Handed Screen Option................................................................................................23
2.3.4 Communications with a Printer or Computer..................................................................................24
2.4 “Push-to-Read” Display Lock .....................................................................................................................25
2.5 Working with HachLink™ ...........................................................................................................................25
2.6 Sample Cell Adapters....................................................................................................................................26
2.6.1 Installing Sample Cell Adapters .........................................................................................................26
2.7 Inserting and Removing Samples................................................................................................................27
2.8 Using the Optional Pour-Thru Cell.............................................................................................................27
2.9 Assembling the Pour-Thru Cell ...................................................................................................................28
2.10 Installing the Pour-Thru Cell......................................................................................................................29
2.11 Using the Pour-Thru Cell............................................................................................................................29
2.11.1 Cleaning the Pour-Thru Cell..............................................................................................................30
2.12 Using the General-Purpose Timer .............................................................................................................30
Section 3 Hach Programs—Simplified Analysis ...........................................................................33
3.1 Selecting a Hach Program.............................................................................................................................33
3.2 Running a Hach Program .............................................................................................................................33
3.3 Using Program Timers ..................................................................................................................................33

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3.4 Taking a Reading............................................................................................................................................33
3.5 Hach Program Options .................................................................................................................................34
3.5.1 Running a Reagent Blank.....................................................................................................................34
3.5.2 Creating a User Program Based on a Hach Program.......................................................................35
3.5.3 Correcting for a Diluted Sample .........................................................................................................35
3.5.4 Changing the Chemical Form .............................................................................................................35
3.5.5 Running a Standard Adjust .................................................................................................................36
3.5.6 Performing Standard Additions .........................................................................................................36
3.6 Adding a Hach Program to the “Favorite Programs” List ......................................................................36
Section 4 Working with Data ...................................................................................................................37
4.1 The Datalog.....................................................................................................................................................37
4.1.1 Storing Data to the Datalog .................................................................................................................37
4.1.2 Recalling, Sending, and Erasing Data from the Datalog.................................................................37
Section 5 User Programs—Customized Analysis ..........................................................................39
5.1 Creating and Saving a New User Program................................................................................................39
5.1.1 Entering a Calibration Formula Directly ...........................................................................................40
5.1.2 Creating a Calibration Table................................................................................................................40
5.2 Recalling a User Program .............................................................................................................................41
5.3 Editing an Existing User Program...............................................................................................................41
5.4 Erasing a User Program ................................................................................................................................41
5.5 Adding User Programs to the “Favorite Programs” List.........................................................................42
Section 6 Standard Additions..................................................................................................................43
6.1 Checking Results with Standard Additions...............................................................................................43
6.2 Using the Standard Additions Option........................................................................................................44
6.3 Estimating Concentration Using Standard Additions..............................................................................45
Section 7 Single Wavelength Mode ......................................................................................................47
7.1 Setting Up Single Wavelength Mode ..........................................................................................................47
7.2 Taking Single Wavelength Measurements .................................................................................................47
Maintenance........................................................................................................................................................49
Section 8 Maintenance.................................................................................................................................51
8.1 Caring for the Instrument.............................................................................................................................51
8.2 Cleaning the Instrument ...............................................................................................................................51
8.3 Replacing the Lamp Module ........................................................................................................................51
8.4 Sample Cell Maintenance .............................................................................................................................51
8.5 Pour-thru Cell Maintenance .........................................................................................................................51
8.6 Alkaline Battery Replacement......................................................................................................................51
8.7 Rechargeable Battery Pack Replacement/Maintenance ..........................................................................52
8.8 Recertification Interval..................................................................................................................................52

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Section 9 Upgrading the Instrument Software ...............................................................................53
9.1 System Requirements ....................................................................................................................................53
9.2 Installing the Upgrade Software on the PC................................................................................................53
9.2.1 Installing from Floppy Disk.................................................................................................................53
9.2.2 Installing from CD-ROM .....................................................................................................................53
9.3 Connecting the Instrument to the Computer.............................................................................................53
9.4 Starting the Upgrade Utility.........................................................................................................................54
9.5 Setting the Communication Link.................................................................................................................54
9.6 Programming the Instrument.......................................................................................................................54
9.7 Uninstalling the Upgrade Software.............................................................................................................54
Section 10 Troubleshooting and FAQs................................................................................................55
10.1 Troubleshooting............................................................................................................................................55
10.1.1 Instrument Information......................................................................................................................55
10.1.2 Wavelength Check ..............................................................................................................................55
10.2 Frequently Asked Questions......................................................................................................................55
General Information.......................................................................................................................................57
Replacement Parts and Accessories......................................................................................................................59
Certification..............................................................................................................................................................60
How To Order ..........................................................................................................................................................62
Repair Service ..........................................................................................................................................................63
Warranty ...................................................................................................................................................................64
Index..........................................................................................................................................................................65

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Safety Information
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Safety Information
Please read this entire manual before unpacking, setting up, or operating this
instrument. Pay particular attention to all danger and caution statements.
Failure to do so could result in serious injury to the operator or damage to the
equipment.
To ensure that the protection provided by this equipment is not impaired, do not
use or install this equipment in any manner other than that which is specified in
this manual.
Hazard Information
If multiple hazards exist, this manual will use the signal word (Danger, Caution,
Note) corresponding to the greatest hazard.
DANGER
Indicates a potentially or imminently hazardous situation which, if not
avoided, could result in death or serious injury.
CAUTION
Indicates a potentially hazardous situation that may result in minor or
moderate injury.
NOTE
Information that requires special emphasis.
Precautionary Labels
Read all labels and tags attached to the instrument. Personal injury or damage to
the instrument could occur if not observed.
This symbol, if noted on the instrument, references this instruction manual
for operational and/or safety information.
Section 2.6 Sample Cell Adapters on page 26
Section 2.7 Inserting and Removing Samples on page 27
Section 2.8 Using the Optional Pour-Thru Cell on page 27
!
!
!
!

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Specifications
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Specifications
Wavelength Range 400 to 880 nm
Bandwidth 4 nm ± 1 nm
Wavelength Accuracy ± 1 nm
Wavelength Resolution 1 nm
Wavelength Selection Automatic, based on method selection
Optical System Concentric Spectrometer for multi-channel spectroscopy
Wavelength Calibration Automatic, via internal filter
Linear Photometric Range –2.000 to +2.000 Abs
Photometric Linearity ±0.005 Abs from 0.0 to 0.5 Abs; ±1% from 0.5 to 2.0 Abs.
Photometric Resolution 0.001 Abs, 0.1 %T
Recommended Recertification Interval One year (See 8.8 Recertification Interval .)
Stray Light >2.5 A, <0.3 %T @ 430 nm using 3 mm thickness GG-475 filter
Operational Modes Selectable; momentary (Display Lock on), or constant (Display Lock off)
Readout Modes Transmittance, absorbance, and concentration
RS232 Port Standard 9-pin bi-directional
Line Power 9 VDC @ 1 amp; 95 to 240 VAC; 50/60 Hz; automatic selection
Battery Power 3 “D” Cell Alkaline or Rechargeable Alkaline Batteries
Sample Compartment and
Cell Compatibility:
Note: For Hach Programs, be sure to use the cell type specified for that
program in the DR/2400 Portable Spectrophotometer Procedures
Manual.
No Cell Holder Required 1-in. (25.4-mm) round cells/vials, including AccuVac®
Round Cell Holder, 16-mm COD/Test ‘N Tube™ and UniCell™ vials
Metric Rectangular Cell Holder (optional) 1-cm rectangular cells
Multi-pathlength Cell Holder Pour-Thru Cell and multi-pathlength cells
Interface Graphic user interface 320 x 240 pixel graphic display with touchscreen
Data Storage 1000 data points (date, time, results, sample ID, user ID), 50 user calibrations
Dimensions 32.4 x 17.8 x 18.8 cm (12.75 x 7.0 x 7.4 in.)
Weight 2.1 kg (4.6 lbs)
Keypad 5 keys for power on/off, backlight on/off, contrast down, contrast up, and battery
status
Printer External serial
Pour-Thru Cell 1-inch/1-cm multi-pathlength (optional)
Report Generation
Download stored information in a standard report format. Meets GLP
guidelines, including a minimum of: date, time, sample identification tag, analyst
initials (3), results, and instrument serial number.
Real-time Clock Ye s
Real-time Calendar Ye s
Operating Environment 0 to 40 °C (32 to 104 °F); 90% relative humidity, non-condensing
Storage Environment –20 to 60 °C (–4 to 140 °F); 85% relative humidity, non-condensing

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Some of the following manual sections contain information in the form of warnings,
cautions and notes that require special attention. Read and follow these instructions
carefully to avoid personal injury and damage to the instrument. Only personnel
qualified to do so, should conduct the installation/maintenance tasks described in this
portion of the manual.
Certains des chapitres suivants de ce mode d’emploi contiennent des informations sous
la forme d’avertissements, messages de prudence et notes qui demandent une attention
particulière. Lire et suivre ces instructions attentivement pour éviter les risques de
blessures des personnes et de détérioration de l’appareil. Les tâches d’installation et
d’entretien décrites dans cette partie du mode d’emploi doivent être seulement
effectuées par le personnel qualifié pour le faire.
Algunos de los capítulos del manual que presentamos contienen información muy
importante en forma de alertas, notas y precauciones a tomar. Lea y siga
cuidadosamente estas instrucciones a fin de evitar accidentes personales y daños al
instrumento. Las tareas de instalación y mantenimiento descritas en la presente sección
deberán ser efectuadas únicamente por personas debidamente cualificadas.
Einige der folgenden Abschnitte dieses Handbuchs enthalten Informationen in Form von
Warnungen, Vorsichtsmaßnahmen oder Anmerkungen, die besonders beachtet werden
müssen. Lesen und befolgen Sie diese Instruktionen aufmerksam, um Verletzungen von
Personen oder Schäden am Gerät zu vermeiden. In diesem Abschnitt beschriebene
Installations- und Wartungsaufgaben dürfen nur von qualifiziertem Personal
durchgeführt werden.
Alcune parti di questo manuale contengono informazioni sotto forma d’avvertimenti,
di precauzioni e di osservazioni le quali richiedono una particolare attenzione. La
preghiamo di leggere attentivamente e di rispettare quelle istruzioni per evitare ogni
ferita corporale e danneggiamento della macchina. Solo gli operatori qualificati per l’uso
di questa macchina sono autorizzati ad effettuare le operazioni di manutenzione
descritte in questa parte del manuale.
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Installation

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Getting Started
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Section 1 Getting Started
1.1 Unpacking the Instrument
The DR/2400 Portable Spectrophotometer comes packaged with one each of the
following items:
•Instrument
•Power cord
•Power supply
•Batteries
•Round sample cell holder assembly
•Procedure Manual with binder
•Instrument Manual
•CD-ROM containing the Procedure and Instrument Manuals
•Set of 6 sample cells, 10 mL/25 mL
If any of these items are missing or damaged, please contact Hach Company or
a sales representative immediately.
Note: Retain the original packaging material. Instruments returned for recertification or service
should be shipped in the original packaging material to protect against damage during
transportation. See Section 8.8 Recertification Interval for more information.
1.2 Operating Environment
Maintain an ambient temperature of 0 to 40 °C (32 to 104 °F) for proper
instrument operation. The relative humidity should be less than 90%; moisture
should not condense on the instrument.
1.3 Cable Connections
Figure 1 shows the cable connections on the back of the instrument.
Figure 1 DR/2400 Cable Connections
Power
RS232
Port

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Getting Started
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1.3.1 Power Connections
A UL/CSA-approved 115-VAC power cord with a NEMA 5-15P style plug is
supplied with the North American DR/2400 models (Cat. No. 18010-00). A
230 VAC harmonized power cord with a Continental European plug is supplied
with the European DR/2400 models (Cat. No. 46836-00).
To power the North American DR/2400 models with 230 VAC, replace the
supplied 115 VAC power cord with a UL/CSA approved 230 VAC power cord
with a NEMA 6-15P style plug.
Plug the power cord into the back panel of the instrument. Connect the power
cord to the proper outlet and press the power key. (See Figure 3.)
1.3.2 Serial Printer and PC Connections
Note: Use of the specified
cable or an equivalent
shielded cable is
mandatory for proper
immunity to
electromagnetic
interferences.
Connect the DR/2400 Spectrophotometer to a personal computer (PC) by using
the computer interface cable (Cat. No. 48129-00). The cable provides a direct
connection between the DR/2400 Spectrophotometer and the 9-pin D connector
used for the serial port on most personal computers. For computers with a
25-pin D connector, use a 9-pin to 25-pin adapter (available at many computer
supply stores).
For proper data transfer, the communication parameters of the DR/2400 and the
computer must match. Refer to Section 2.3.4 Communications with a Printer or
Computer for selecting DR/2400 printer and computer settings.
Once the communication link is established, touch the Send to Printer or Send to
Computer icon to send data to the printer or computer.
1.4 Battery Pack
The DR/2400 Spectrophotometer is equipped with 3 D-cell alkaline batteries to
provide primary power for field operation. The alkaline batteries can supply the
instrument with power to remain in operation for up to 200 hours.
Note: The rechargeable pack can provide power to the instrument for up to 40 hours before
recharging.
Changing the Batteries
When the alkaline batteries need to be replaced, remove the battery cover on the
bottom of the instrument. Remove the discharged batteries and replace with
fresh D-cell alkaline batteries. Orient all three batteries so that the positive
terminals point to the keyboard (front) end of the instruments. See Figure 2 on
page 15.
The Rechargeable Battery Pack is replaced as a unit. Remove the discharged
pack and replace with the fresh pack. Orient the pack so that the positive
terminals point to the keyboard (front) end of the instrument.
Note: The Rechargeable Battery Pack (Cat. No. 59491-00) is available separately. The alkaline
rechargeable batteries in the rechargeable pack are not individually replaceable.

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Figure 2 Changing the Batteries
The internal clock loses its setting whenever batteries are replaced. To reset the
clock, see Setting the Time and Date on page 21
1.5 Using the Instrument Keys
The instrument keys are shown in Figure 3.
Figure 3 Instrument Keys
1.5.1 Turning the Power On and Off
Turn the instrument on and off with the Power On/Off key (see Figure 3). The first
time the instrument is turned on, the language selection screen will appear.
Select a language and touch OK. At each succeeding instrument power-up, a
wavelength calibration will be performed automatically, and then the Main
Menu will appear.
Battery Status
Power On/Off
Backlight On/Off Contrast Adjustment

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1.5.2 Turning On the Backlight
The backlight can improve the readability of the screen in low-light conditions.
Press the Backlight key to turn the light on or off. The light will automatically
shut off 15 minutes after the last key has been pressed.
1.5.3 Adjusting the Display Contrast
Lighten or darken the contrast on the display using the Contrast Adjustment keys.
1.5.4 Battery Status
Press the Battery Status key to bring up the battery meter reading window. Press
the Battery Status key again to return to the previous screen menu. The Battery
Status reading window is not available from all screens. For more information
about batteries, see Specifications on page 9, Alkaline Battery Replacement on page 51,
and Rechargeable Battery Pack Replacement/Maintenance on page 52.
Figure 4 Battery Status–Line Power
Figure 5 Battery Status–Battery Power

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Figure 6 Battery Status–Charging
1.6 Tips for Using the Touch Screen
The entire screen is touch-activated. Spend a few minutes touching various items
on the screen to see how they work.
•To make a selection, touch the screen with a fingernail, fingertip, pencil
eraser, or a stylus. (See Figure 7.)
•Do not touch the screen with a sharp object, such as the tip of a ball
point pen!
•Touch buttons, words, or icons to select them.
•Touch buttons or check boxes to change settings.
•Use scroll bars to move up and down long lists very quickly. Touch and hold
the scroll bar, then move the fingertip up or down to move through the list.
•Highlight an item from a list by touching it once. When the item has been
successfully selected, it will be displayed as reversed text (light text on a dark
background).
Figure 7 Different Touch Screen Navigation Options
1.7 The DR/2400 Portable Spectrophotometer Main Menu
The Main Menu appears when the instrument is turned on. Figure 8 shows the
Main Menu on the standard software package. Figure 8 shows the Main Menu on
the advanced software package.

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Figure 8 Main Menu–Standard Software
1.8 Using the DR/Check™ Absorbance Standards
Hach’s DR/Check Absorbance standards are used in instrument performance
verification on both the DR/2400 and DR/800 Series instruments. The set
consists of three standards plus a blank. Each of the standards is certified to give
a known absorbance value at a particular wavelength. The standards can be
used to verify that the instrument is performing in a consistent manner on a
routine basis.
1.8.1 How to Use the Standards
Note: Be sure to follow the cell alignment steps found on the product box.
The DR/Check Standards are used to verify instrument performance by
measuring a specific absorbance at each of the following wavelengths:
•420
•520
•560
•610
1. From the Main Menu screen, touch Single Wavelength.
2. From the Single Wavelength screen, touch λ.
3. Key in 420 nm.
4. Insert the blank into the instrument so that it is properly aligned with the light
source. Touch Zero.
Note: For best results, wipe all the cells with a clean tissue and silicone oil before use
5. Place Standard #1 in the instrument so that it is properly aligned. Read the
absorbance.
6. Record the initial instrument reading in the blank table provided with the
standards. The initial value should be within the tolerance limits of the
certified absorbance specified on the Certificate of Analysis for the DR/Check
Standards.
Note: If values are not within specified tolerance limits, contact Hach Technical Support. See
Repair Service on page 63 for contact information.
7. Repeat steps 2 through 6for the other two standards, using the correct
wavelength in step 3.
Note: To continuously monitor instrument performance, repeat all steps and compare readings to
the recorded values.

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1.9 Determining Photometric Accuracy
A verification of photometric accuracy can be performed on the DR/2400 using
Hach DR/Check (Cat. No. 27639-00), NIST SRM 930 neutral density glass, or
NIST SRM 2031 metal-on-quartz standard reference materials.
To verify the photometric performance of the DR/2400 with standards, the
instrument zero should be performed on a “like” standard to obtain the
maximum performance capability of the instrument. Using the DR/Check, a
blank is performed on the blank provided with the DR/Check set and values of
standards are read directly. When using NIST standards or equivalent glass
standards, a blank is performed on the non-absorbing reference standard
provided with the set. If the stated values for the standards include the reflection
loss, then the stated value of the non-absorbing standard is subtracted from each
of the absorbance standards to obtain an adjusted true value for the reference
standards. It is these adjusted values that are used to compare the photometric
accuracy of the DR/2400 when the instrument is blanked on material other than
air. The following example provides a method for checking photometric
accuracy using glass standards with the DR/2400 that most closely matches the
performance obtained when a blank is used in Hach water analysis methods.
Example:
1. Turn the DR/2400 on and set the appropriate wavelength (see How to Use the
Standards on page 18 for setting the wavelength). The wavelength should be
set to the stated value of the reference standard being used.
2. Set the instrument to read in the same units as the reference standards
(usually “Abs” units).
3. Insert the blank or non-absorbing reference standard into the DR/2400 and
zero the instrument. The display should read 0.000 Abs.
4. Remove the blank and insert a reference standard, such as the 0.250 Abs
reference standard.
5. Touch Read to get the value for the reference standard. This is the “read”
value for the reference standard. An average of ten readings should be used to
obtain the “read” value.
6. Calculate an adjusted value for the 0.250 Abs standard by subtracting the
stated value of the non-absorbing reference standard from the stated value of
the 0.250 Abs standard. If the stated value for the non-absorbing standard is
0.0374 and the stated value for the 0.250 Abs standard is 0.2529, then the
adjusted value for the 0.250 Abs standard is:
0.2529 - 0.0374 = 0.2155 Abs.
7. Compare the “read” values to the adjusted values for the standard.
8. The standard deviation of the ten “read” values is the precision of the
DR/2400.
9. Report the difference between the “read” value and “adjusted” value plus or
minus the standard deviation.
10. Repeat steps 4 through 9 for reference standards of other values or
wavelengths.

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