Halma Ocean Optics Maya2000Pro Series User manual

000-00000-000-02-A
Maya2000Pro Series Spectrometers
Installation and Operation Manual
For Products: Maya 2000Pro and
Maya2000Pro-NIR
Document: 020-00000-000-02-201603b

AMERICAS & WORLD HEADQUARTERS
Phone: [phone]
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Sales: [email protected]
Orders: [email protected]
Support: [email protected]
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www.oceanoptics.com
Copyright © 2009 Ocean Optics, Inc.
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic,
mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
Trademarks
All products and services herein are the trademarks, service marks, registered trademarks or registered service marks of their respective owners.
Limit of Liability
Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information
provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or
damages arising from the information contained in this manual.
Ocean Optics, Inc.
830 Douglas Ave.
Dunedin, FL 34698
USA
Manufacturing & Logistics
4301 Metric Dr.
Winter Park, FL 32792
USA
Ocean Optics Asia
666 Gubei Road
Kirin Tower Suite 601B
Changning District
Shanghai
PRC, 200336
Sales & Support
Geograaf 24
6921 EW Duiven
The Netherlands
Manufacturing & Logistics
Maybachstrasse 11
73760 Ostfildern
Germany

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Table of Contents
About This Manual.........................................................................................................iii
Document Purpose and Intended Audience..............................................................................iii
What’s New in this Document ...................................................................................................iii
Document Summary..................................................................................................................iii
Product-Related Documentation ...............................................................................................iii
Upgrades.......................................................................................................................iv
Chapter 1: Introduction .....................................................................1
Product Overview..........................................................................................................1
Models...........................................................................................................................2
Features........................................................................................................................2
System Requirements ...................................................................................................3
EEPROM Utilization ..................................................................................................................3
About OceanView Software.......................................................................................................3
Sampling System Overview.......................................................................................................3
How Sampling Works............................................................................................................3
Modular Sampling Accessories.............................................................................................4
Interface........................................................................................................................4
Shipment Components..................................................................................................4
Other Accessories Available..........................................................................................5
Breakout Box.............................................................................................................................5
Chapter 2: Installing the Maya2000Pro Series Spectrometers.......7
Overview .......................................................................................................................7
Maya2000Pro Series Installation...................................................................................7
Configuring the Maya2000Pro Series Spectrometers ....................................................8
OceanView QE Pro Acquisition Controls..............................................................................8
Connect Spectroscopic Accessories..............................................................................10
Chapter 3: Troubleshooting..............................................................13
Overview .......................................................................................................................13
Maya2000Pro Series Spectrometers Connected to Computer Prior to Software Installation
......................................................................................................................................13

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Windows Operating Systems ....................................................................................................13
Remove the Unknown Device from Windows Device Manager ...........................................13
Mac Operating Systems............................................................................................................14
Linux Operating Systems ..........................................................................................................14
Appendix A: Calibrating the Wavelength of the Maya2000Pro Series
Spectrometers....................................................................................15
Overview .......................................................................................................................15
About Wavelength Calibration .......................................................................................15
Calibrating the Spectrometer.........................................................................................16
Preparing for Calibration............................................................................................................16
Calibrating the Wavelength of the Spectrometer ......................................................................16
Saving the New Calibration Coefficients: USB Mode.....................................................18
Appendix B: Specifications...............................................................19
Overview .......................................................................................................................19
How the Maya2000Pro Series Works............................................................................19
Maya2000Pro Series Components Table .................................................................................20
Maya2000Pro Series Spectrometers Specifications ......................................................21
CCD Detector Specifications.....................................................................................................21
Maya2000Pro Series Spectrometer Specifications...................................................................22
System Compatibility.....................................................................................................23
30-Pin Accessory Connector Pinout ..............................................................................23
30-Pin Accessory Connector Pinout Diagram...........................................................................24
30-Pin Accessory Connector –Pin Definitions and Descriptions..............................................24
30-Pin J2 Accessory Connector - Part Numbers.......................................................................26
Index...................................................................................................27

020-00000-000-02-201603b iii
About This Manual
Document Purpose and Intended Audience
This document provides the user of a Maya2000Pro Series Spectrometer with instructions for setting up,
calibrating and performing experiments with their spectrometer.
What’s New in this Document
This version of the Maya2000Pro and Maya2000Pro-NIR Installation and Operation Manual adds
information for the shutter and interchangeable slits.
Document Summary
Chapter
Description
Chapter 1: Introduction
Contains descriptive information about the Maya2000Pro Series
Spectrometers and how sampling works. It also provides a list of
system requirements, interface options, and shipment
components.
Chapter 2: Installing the
Maya2000Pro Series
Provides installation and configuration instructions.
Chapter 3: Troubleshooting
Contains recommended steps to isolate and correct common
problems.
Appendix A: Calibrating the
Wavelength of the Maya2000Pro
Series
Provides instructions for calibrating the Maya2000Pro Series
Spectrometers.
Appendix B: Specifications
Contains technical specifications and connector pinouts for the
Maya2000Pro Series Spectrometers.
Product-Related Documentation
You can access documentation for Ocean Optics products by visiting our website at
http://www.oceanoptics.com. Select Technical Operating Instructions, then choose the appropriate
document from the available drop-down lists.
Document for…
Document Location
OceanView software
http://oceanoptics.com///wp-content/uploads/OceanViewIO.pdf

About This Manual
iv 020-00000-000-02-201603b
Document for…
Document Location
SpectraSuite software
http://oceanoptics.com/wp-content/uploads/SpectraSuite.pdf
HR-4 Breakout Box
http://oceanoptics.com/wp-content/uploads/HR-4-Breakout-Box.pdf
External triggering for
firmware versions below
3.0
http://oceanoptics.com/wp-content/uploads/External-Triggering-Options.pdf
External triggering for
firmware versions 3.0 and
above
http://oceanoptics.com/wp-content/uploads/External-Triggering-
Options_Firmware3.0andAbove.pdf
Ocean Optics offers a Glossary of spectroscopy terms to help you further understand your state-
of-the-art products and how they function, located at: http://oceanoptics.com/glossary/.
Upgrades
Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a
product.
Our 3-Year Warranty covers Ocean Optics miniature fiber optic spectrometers, light sources and
sampling accessories –regardless of the application –from manufacturing defects. It also covers fibers
and probes for a full 12 months: http://oceanoptics.com/services/exclusive-3-year-warranty/.
This comprehensive warranty ensures you of the highest level of craftsmanship and reliability for years to
come. No other manufacturer offers such a solid guarantee of quality and reliability.
The Ocean Optics 3-Year Warranty applies to Ocean Optics equipment (excluding OEM configurations)
purchased on or after July 1, 2010. The warranty covers parts and labor needed to repair manufacturing
defects that occur during the warranty period. We also will cover the costs of shipping warranty-related
repairs from our customers to Ocean Optics and from us to our customers.
ISO Certification
Ocean Optics, the industry leader in miniature photonics, has been certified for ISO 9001:2008
certification applicable to the design and manufacture of electro-optical equipment since 2009.

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WARNING
This is a Class A product. In a domestic environment this product may cause radio
interference in which case the user may be required to take adequate measures.
FCC COMPLIANCE
This equipment has been tested and found to comply with the limits for a Class A
digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to
provide reasonable protection against harmful interference when the equipment is
operated in a commercial environment. This equipment generates, uses and can
radiate radio frequency energy and, if not installed and used in accordance with the
instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful
interference in which the user will be required to correct the interference at his on
expense.
WARNING: The authority to operate this equipment is conditioned by the
requirement that no modifications will be made to the equipment unless the changes
or modifications are expressly approved by the manufacturer.

About This Manual
vi 020-00000-000-02-201603b

020-00000-000-02-201603b 1
Chapter 1
Introduction
Product Overview
The Ocean Optics Maya2000Pro Series Spectrometers have been engineered specifically for low light-
level, UV-sensitive and other scientific applications such as fluorescence, DNA sequencing and Raman
spectroscopy. These next-generation spectrometers have great quantum efficiency, high dynamic range,
extreme sensitivity and excellent deep-UV (<190 nm) response.
Ocean Optics Maya2000Pro High-Sensitivity Fiber Optic Spectrometer
The Maya2000Pro Series Spectrometers feature the Hamamatsu FFT-CCD back-thinned detector, which
offers excellent performance characteristics. Because of their great native UV-response, FFT-CCD
detectors do not require UV-sensitive coatings, eliminating batch-to-batch variations. Other performance
advantages of this detector include great signal-to-noise characteristics, low dark current and good signal
processing speed.
The Maya2000Pro Series’ onboard module has 10 user-programmable digital I/O lines for interfacing to
other equipment; and a pulse generator for triggering other devices. You can use the I/Os to flash a lamp,
stop/start a process, and send a message/alarm during the spectrometer’s integration period. The
spectrometer’s operating parameters can be controlled through software. In fact, wavelength calibration
coefficients unique to each spectrometer are programmed into a memory chip right on the spectrometer.
TheMaya2000Pro Series’ high-speed electronics have been designed for considerable flexibility in
connecting to various modules and external interfaces, including PCs, PLCs and other embedded
controllers, through USB 2.0 communications. Its USB 2.0 interface enables full spectral scans into
memory every 7 milliseconds.

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An optional internal shutter is available on custom-configured spectrometers (INTERNAL-SHUTTER-
LRG-BENCH). This makes it easier to take dark measurements and when you need fast integration times
with the best possible signal. This optional feature is also recommended for probe-based or emissive
measurements where it is difficult to introduce a shutter into the optical path. The shutter can be added to
an existing Maya spectrometer for an extra charge.
Replaceable slits are offered for added convenience. ). Deep UV option includes purge port.
The Maya2000Pro Series Spectrometers operate via USB interface.
Models
The Maya2000Pro Series Spectrometers consist of the Maya2000 Pro and the Maya2000Pro-NIR. The
Maya2000 has been discontinued. While these spectrometers have similar performance for most
parameters, the May2000Pro -NIR offers high sensitivity in the NIR range while the Maya2000Pro offers
high sensitivity in the UV range. See Maya2000Pro Series Spectrometers Specifications for a side-by-
side comparison.
Features
Hamamatsu S10420 (Maya2000Pro), or Hamamatsu S11510 (Maya2000Pro-NIR) Detector
Peak QE: 75% for Maya2000 Pro; 85% for Maya2000Pro-NIR
Back-thinned for good UV sensitivity
MPP operation for low noise
Spectrometer Design
Symmetrical Crossed Czerny Turner
101mm focal length
14 grating options, including the HC-1 composite grating for coverage from 175-1100 nm
(additional charge)
6 slit widths
Interchangeable slits
Kensington®security slot
Optional shutter for dark measurements requiring a fast integration time and good throughput –
Specify when ordering
Electrical Performance
16 bit, 500KHz A/D converter
Integration times: 6ms to 5sec for Maya2000 Pro
Embedded microcontroller allows programmatic control of all operating parameters and
standalone operation
USB 2.0 480Mbps (high-speed) & 12Mbps (full speed)
Multiple communication standards for digital accessories (SPI, I2C)
Onboard Pulse Generator

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2 programmable strobe signals for triggering other devices
Software control of nearly all pulse parameters
Onboard GPIO
10 user-programmable digital I/Os
EEPROM storage for
Wavelength Calibration Coefficients
Linearity Correction Coefficients
Absolute Irradiance Calibration (optional)
Plug-n-play interface for PC applications
30-pin connector for interfacing to external products
CE certification
System Requirements
You can use the Maya2000Pro Series’USB connectivity with any computer that meets the requirements
for the spectrometer operating software being used (Windows 98/Me/2000/XP, Mac OS X and Linux).
See About OceanView Software.
EEPROM Utilization
An EEPROM memory chip in each Maya2000Pro Series contains wavelength calibration coefficients,
linearity coefficients, and a serial number unique to each individual spectrometer. The OOI software
application reads these values directly from the spectrometer, enabling the ability to “hot-swap”
spectrometers between computers without entering the spectrometer coefficients manually on each
computer.
About OceanView Software
OceanView is the latest generation of operating software for all Ocean Optics spectrometers. It is a
completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh
and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device.
OceanView is a user-customizable, advanced acquisition and display program that provides a real-time
interface to a variety of signal-processing functions. With OceanView, you have the ability to perform
spectroscopic measurements (such as absorbance, reflectance, and emission), control all system
parameters, collect and display data in real time, and perform reference monitoring and time acquisition
experiments. Consult the OceanView manual for hardware requirements when using OceanView (see
Product-Related Documentation).
Sampling System Overview
How Sampling Works
Ocean Optics components function in a sampling system as follows:

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4020-00000-000-02-201603b
1. The user stores reference and dark measurements to correct for instrument response variables.
2. The light transmits through an optical fiber to the sample.
3. The light interacts with the sample.
4. Another optical fiber collects and transmits the result of the interaction to the spectrometer.
5. The spectrometer measures the amount of light and transforms the data collected by the
spectrometer into digital information.
6. The spectrometer passes the sample information to OOI software.
7. OOI software compares the sample to the reference measurement and displays processed spectral
information.
Modular Sampling Accessories
Ocean Optics offers a complete line of spectroscopic accessories for use with the Maya2000Pro Series.
Most of our spectroscopic accessories have SMA connectors for application flexibility. Accordingly,
changing the sampling system components is as easy as unscrewing a connector and replacing an
accessory.
Interface
You can use the Maya’s USB connectivity with any computer that meets the following requirements:
Microsoft Windows –Windows 2000/XP/7; 32-bit and 64-bit and Windows Vista (32-bit only)
Apple Macintosh –OS X version 10.0 or later
Linux –Any version released for an x86 or amd64 platform since 2010
Shipment Components
Maya2000Pro Series Spectrometer
The following information and documentation also ships with the Maya2000Pro Series Spectrometer:
Packing List
The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice
arrives separately). It lists all items in the order, including customized components in the
spectrometer (such as the grating, detector collection lens, and slit). The packing list also includes
the shipping and billing addresses, as well as any items on back order.
Wavelength Calibration Data Sheet
Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information
unique to your spectrometer. Your spectrometer operating software reads this calibration data
from your spectrometer when it interfaces to a computer via the USB port.
Note

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Please save the Wavelength Calibration Data Sheet for future reference.
Other Accessories Available
Visit us at www.OceanOptics.com for a complete list of products available for all of your spectroscopy
needs.
Fibers
Light Sources
Integrated Sampling Systems
Cuvettes
Filter Holders
Lithium Ion Battery Pack
HR4-BREAKOUT Breakout Box
Breakout Box
Ocean Optics also offers the Breakout Box (HR4-BREAKOUT), a passive module that separates the
signals from their 22-pin port to an array of standard connectors and headers, enabling easy access to a
variety of features found in Ocean Optics’ Maya2000Pro Series Spectrometer. In addition to the
accessory connector, the breakout box features a circuit board based on a neutral breadboard pattern that
allows custom circuitry to be prototyped on the board itself.

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Chapter 2
Installing the Maya2000Pro
Series Spectrometers
Overview
You must install the operating software application prior to connecting your Maya2000Pro Series
Spectrometer to a computer. The Ocean Optics OceanView spectrometer operating software installs the
drivers required for the Maya2000Pro Series spectrometer installation. If you do not install the software
first, the system will not properly recognize the Maya2000Pro Series.
If you have already connected the Maya2000Pro Series to the computer prior to installing the operating
software, consult Chapter 3: Troubleshooting for information on correcting a corrupt Maya2000Pro
Series installation.
Maya2000Pro Series Installation
This section contains instructions for connecting the Maya2000Pro Series Spectrometer to a computer.
►Procedure
Follow the steps below to connect the Maya2000Pro Series to a computer via the USB port:
1. Install the spectrometer operating software on the destination computer.
2. Locate the USB cable (USB-CBL-1) provided with the Maya2000Pro Series Spectrometer.
3. Insert the square end of the cable into the side of the spectrometer.
4. Insert the rectangular end of the cable into the USB port of the computer.
If you installed OceanView prior to connecting the spectrometer, OceanView installs the spectrometer
drivers. If the drivers do not successfully install (or if you connected the spectrometer to the computer
before installing OceanView), consult Chapter 3: Troubleshooting.
Once you install the software and hardware, and establish your sampling system, you are ready to take
measurements.

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Configuring the Maya2000Pro Series
Spectrometers
The Maya2000Pro Series spectrometer can be used with OceanView software when connected to the
USB port.
If you have followed the previous steps and started your software, the spectrometer is already acquiring
data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of
the graph. If you allow light into the spectrometer, the graph trace should rise with increasing light
intensity. This means the software and hardware are correctly installed.
Consult your spectrometer operating software manual for detailed instructions on operating your
spectrometer (see Product-Related Documentation).
OceanView QE Pro Acquisition Controls
In OceanView, the Acquisition Parameter Controls allow you to set the desired parameters for the Maya.
Optional Shutter
►Procedure
To enable the optional shutter,

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1. In the Acquisition Group window, click on the Add/Remove Controls tab.
2. Select GPIO. The GPIO controls appear on the Main Controls tab.
3. Check the Out box for Index 4. This makes the GPIO an output.
4. Check the Value box for Index 4 to close the shutter. You can now take your dark measurement.
5. Uncheck the Value box to open the shutter.

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Notes
Make sure the ALT checkbox for the GPIO (Index 4) is not selected, which would
prevent the shutter from triggering.
The activation time of the internal shutter is 11 ms.
Connect Spectroscopic Accessories
To find operating instructions for Maya2000Pro Series-compatible products (such as light sources,
sampling chambers, and probes), consult the Ocean Optics website at
http://www.oceanoptics.com/technical/operatinginstructions.asp.
Change the Slit
There’s no need to calibrate your spectrometer when changing the slit, just install and start measuring.
There is one exception to this. You cannot change from a standard slit to a slit with a filter because it
changes the optical focus and wavelength calibration of the spectrometer. In this case you would need to
send the spectrometer back to Ocean Optics. A filter must be ordered for each slit (if your application
requires a filter) and the spectrometer needs to be calibrated and focused with the filter installed. This
only applies to filters installed inside the slit assembly.
►Procedure
1. Find the SMA connector. If a fiber is attached, remove it.

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2. Use the Allen key to remove the 2 the screws attaching the slit to the spectrometer.
3. Pull the slit out of the spectrometer.
4. Put the new INTSMA slit connector into the spectrometer; with the key of the connector on the
left side.
5. Install the 2 screws again. Use the Allen key to tighten the screws carefully (do not over-tighten).
6. If necessary, connect the fiber again.

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