Halma Ocean Optics HR2000 Series User manual

HR2000 and HR2000CG-UV-NIR Series
High-Resolution Fiber Optic
Spectrometers
HR2000 / HR2000CG-UV-NIR
Installation and Operation Manual
Document Number 194-00000-000-02-0305
Offices: Ocean Optics, Inc.
830 Douglas Ave., Dunedin, FL, USA 34698
Phone [phone]
Fax [phone]
8:30 a.m.-6 p.m. EST
Ocean Optics B.V. (Europe)
Nieuwgraaf 108 G, 6921 RK DUIVEN, The Netherlands
Phone [phone]
F
ax 31-(0)26-3190505
E-mail: [email protected] (General sales inquiries)
[email protected] (European sales inquiries)
[email protected] (Questions about orders)
[email protected] (Technical support)

Copyright © 2001-2005 Ocean Optics, Inc.
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic,
mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or
otherwise circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.
Trademarks
Microsoft, Windows, Windows 95, Windows 98, Windows Me, Windows NT, Windows 2000, Windows XP and Excel are either registered
trademarks or trademarks of Microsoft Corporation.
Limit of Liability
Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The
information provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect
to any loss or damages arising from the information contained in this manual.

Table of Contents
Chapter 1: HR2000 Introduction...........................................................................................................1
Product Overview .................................................................................................................................................. 1
System Requirements...................................................................................................................................................................................1
EEPROM Utilization......................................................................................................................................................................................2
About OOIBase32.........................................................................................................................................................................................2
Sampling System Overview ..........................................................................................................................................................................2
Modular Sampling Accessories.....................................................................................................................................................................2
Interface Options ................................................................................................................................................... 3
Shipment Components.......................................................................................................................................... 3
Packing List...................................................................................................................................................................................................3
Wavelength Calibration Data Sheet..............................................................................................................................................................3
Software and Technical Resources CD ........................................................................................................................................................4
Other Documentation....................................................................................................................................................................................4
Modification and Repair Policy......................................................................................................................................................................4
Chapter 2: HR2000 Specifications........................................................................................................5
How the HR2000 Works........................................................................................................................................ 5
HR2000 Specifications Table........................................................................................................................................................................6
HR2000 Specifications .......................................................................................................................................... 7
CCD Detector Specifications.........................................................................................................................................................................7
HR2000 Spectrometer Specifications ...........................................................................................................................................................7
System Compatibility.....................................................................................................................................................................................8
20-Pin Accessory Connector Pinout..............................................................................................................................................................8
20-Pin Accessory Connector Pinout Diagram...............................................................................................................................................9
20-Pin Accessory Connector – Pin Definitions..............................................................................................................................................9
20-Pin J2 Accessory Connector - Part Number and Compatibility................................................................................................................9
Chapter 3: Installing the HR2000........................................................................................................10
HR2000 Installation ............................................................................................................................................. 10
Configuring the HR2000 in OOIBase32 ......................................................................................................................................................11
Connect Spectroscopic Accessories...........................................................................................................................................................13
External Triggering Options................................................................................................................................. 13
Chapter 4: Troubleshooting................................................................................................................14
HR2000 Connected to PC Prior to OOIBase32 Installation................................................................................ 14
Remove the Unknown Device from Windows Device Manager..................................................................................................................14
Windows 98:................................................................................................................................................................................................15
Windows 2000/XP:......................................................................................................................................................................................16
Troubleshooting the Serial Port Configuration..................................................................................................... 17
Older Version of OOIBase32 Installed ................................................................................................................ 17

Chapter 5: Sample Experiments.........................................................................................................18
Preparing for Experiments................................................................................................................................... 18
Absorbance Experiments..................................................................................................................................... 20
Transmission Experiments .................................................................................................................................. 22
Reflection Experiments........................................................................................................................................ 24
Relative Irradiance Experiments.......................................................................................................................... 26
Time Acquisition Experiments............................................................................................................................. 28
Appendix A: Calibrating the Wavelength of the HR2000 .................................................................31
About Wavelength Calibration............................................................................................................................. 31
Calibrating the Spectrometer............................................................................................................................... 31
Preparing for Calibration .............................................................................................................................................................................31
Calibrating the Wavelength of the Spectrometer.........................................................................................................................................32
Saving the New Calibration Coefficients: USB Mode..................................................................................................................................34
Saving the New Calibration Coefficients: Serial Mode................................................................................................................................35
Appendix B: HR2000CG-UV-NIR Spectrometer ................................................................................36
HR2000CG-UV-NIR Features ............................................................................................................................. 36
HR2000CG-UV-NIR Specifications.............................................................................................................................................................36
Index ......................................................................................................................................................37

HR2000 Introduction
HR2000 High-Resolution Fiber Optic Spectrometer 1
1 HR2000 Introduction
The following chapter contains introductory information about the HR2000 Spectrometer, shipment information,
and spectrometer connectivity.
Product Overview
The HR2000 High-resolution Miniature Fiber Optic Spectrometer provides optical resolution as good as 0.065 nm
(FWHM). The HR2000 is responsive from 200-1100 nm, but the specific range and resolution depends on your
grating and entrance slit selections.
The HR2000 is perfect for applications where high resolution is necessary, such as absorbance of gases or
atomic emission lines (for solution chemistry or for color measurements, the S2000, PC2000 and USB2000 are
more appropriate spectrometers).
Data programmed into a memory chip on each HR2000 includes wavelength calibration coefficients, linearity
coefficients and the serial number unique to each spectrometer. Our spectrometer operating software simply
reads these values from the spectrometer -- a feature that enables hot swapping of spectrometers among PCs.
The HR2000 Spectrometer connects to a notebook or desktop PC via USB port or serial port. When connected to
the USB port of a PC, the HR2000 draws power from the host PC, eliminating the need for an external power
supply.
Figure 1-1: Ocean Optics HR2000 High-Resolution Fiber Optic Spectrometer
System Requirements
You can use the HR2000’s USB connectivity with any PC that meets the following requirements:
• Windows 98/Me/2000/XP operating system (or Windows CE 2.11 or later for handheld PCs)
• Ocean Optics OOIBase32 software application installed and configured for use with the HR2000
(OOIPS2000 software if using a handheld PC). Consult the “Configuring the HR2000 in OOIBase32”
section of Chapter 3: Installing the HR2000 for specific configuration instructions.
Alternately, the HR2000 has serial port adaptability for connecting to PCs, PLCs, and other devices that support
the RS-232 communication protocol. However, this connection method requires an external power supply to
power the HR2000, as well as a serial port adapter.

HR2000 Introduction
HR2000 High-Resolution Fiber Optic Spectrometer 2
EEPROM Utilization
An EEPROM memory chip in each HR2000 contains wavelength calibration coefficients, linearity coefficients, and
a serial number unique to each individual spectrometer. The OOIBase32 software application reads these values
directly from the spectrometer, enabling the ability to “hot-swap” spectrometers between PCs without entering the
spectrometer coefficients manually on each PC.
About OOIBase32
OOIBase32 is the latest generation of operating software for all Ocean Optics spectrometers and is available free
to all customers. OOIBase32 is a user-customizable, advanced acquisition and display program that provides a
real-time interface to a variety of signal-processing functions. With OOIBase32, you have the ability to perform
spectroscopic measurements (such as absorbance, reflectance, and emission), control all system parameters,
collect and display data in real time, and perform reference monitoring and time acquisition experiments.
Note: When using a handheld PC, you will use the OOIPS2000 software instead of OOIBase32. The
functionality of OOIPS2000 is similar to OOIBase32, but it is specifically for the handheld PC.
Sampling System Overview
Ocean Optics fiber optic spectrometer systems consist of low-cost, modular data acquisition components. A
typical HR2000-based sampling system contains four core elements:
• HR2000 Spectrometer
• OOIBase32 operating software
• Light source
• Sampling optics (varying, depending on application requirements)
How Sampling Works
The following list explains the function of Ocean Optics sampling components in the sampling process:
1. The user stores reference and dark measurements to correct for instrument response variables.
2. The light from the light source transmits through an optical fiber to the sample.
3. The light interacts with the sample.
4. Another optical fiber collects and transmits the result of the interaction to the spectrometer.
5. The spectrometer measures the amount of light and transforms the data collected by the spectrometer
into digital information.
6. The spectrometer passes the sample information to OOIBase32.
7. OOIBase32 compares the sample to the reference measurement and displays processed spectral
information.
Modular Sampling Accessories
Ocean Optics offers a complete line of spectroscopic accessories for use with the HR2000. Most of our
spectroscopic accessories have SMA connectors for application flexibility. Accordingly, changing the sampling
system components is as easy as unscrewing a connector and replacing an accessory.

HR2000 Introduction
HR2000 High-Resolution Fiber Optic Spectrometer 3
Interface Options
The HR2000 has both USB and serial port connectors (with the use of an adapter), enabling you to connect the
spectrometer to a desktop or notebook PC via a USB port or to a desktop, notebook, or to a handheld PC via a
serial port.
Computer
Interface Operating System Requirements Part Needed Description of Part
Desktop or
Notebook PC
via USB Port
Windows 98/Me/
2000/XP USB-CBL-1 (included) Cable that connects from USB port on HR2000 to USB port on desktop or notebook PC
Desktop or
Notebook PC
via Serial Port
Any 32-bit
Windows operating
system
USB-ADP-PC
(not included)
Adapter block that enables connection from serial port on
HR2000 to serial port on desktop or notebook PC; comes with 5
VDC power supply (required when connecting to serial port)
Handheld PC
via Serial Port Windows CE 2.11 or higher USB-ADP-H (not included)
Adapter block that enables connection (with standard 9-pin
serial cable) from serial port on HR2000 to serial port on
handheld PC; comes with 5 VDC power supply (required when
connecting to serial port)
Shipment Components
The following information and documentation ships with the HR2000 Spectrometer:
Packing List
The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice arrives separately).
The packing slip lists all items in the order, including customized components in the spectrometer (such as the
grating, detector collection lens, and slit). The packing list also includes the shipping and billing addresses, as well
as any items on back order.
Wavelength Calibration Data Sheet
Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information unique to your
spectrometer. OOIBase32 Operating Software reads this calibration data from your spectrometer when it
interfaces to a PC via the USB port. Any other interface requires that you manually enter the calibration data in
OOIBase32 (select Spectrometer | Configure | Wavelength Calibration tab). See the OOIBase32
documentation for more information (refer to Other Documentation for instructions on accessing OOIBase32
documentation).
Note: Please save the Wavelength Calibration Data Sheet for future reference.

HR2000 Introduction
HR2000 High-Resolution Fiber Optic Spectrometer 4
Software and Technical Resources CD
Each order ships with the Ocean Optics Software and Technical Resources CD. This disc contains software,
operating instructions, and product information for all Ocean Optics software, spectrometers, and spectroscopic
accessories. You will need Adobe Acrobat Reader version 6.0 or higher to view these files. Ocean Optics includes
Adobe Acrobat Reader on the Software and Technical Resources CD.
With the exception of OOIBase32 Spectrometer Operating Software, all Ocean Optics software requires a
password during the installation process. You can locate passwords for the other software applications on the
back of the Software and Technical Resources CD package.
Other Documentation
You can find detailed instructions for the OOIBase32 Spectrometer Operating Software at
http://www.oceanoptics.com/technical/operatinginstructions.asp.
Note: You can find instructions for the OOIPS2000 Operating Software for the handheld PC at the following
location: http://www.oceanoptics.com/products/ooips2000.asp.
To find operating instructions on some of Ocean Optics’ most popular spectroscopic accessories (including light
sources, sampling chambers, sampling optics, and probes), consult the operating instructions for Ocean Optics
products from the Software and Technical Resources CD that ships with the product, or visit our website at
http://www.oceanoptics.com/technical/operatinginstructions.asp.
Modification and Repair Policy
In the event that you need to return an item for modification or service, you must obtain a Return Merchandise
Authorization (RMA) number prior to shipping the item back to Ocean Optics. Contact an Ocean Optics
Applications Sales Engineer for specific instructions on returning an item.

HR2000 Specifications
HR2000 High-Resolution Fiber Optic Spectrometer 5
2 HR2000 Specifications
This chapter contains information on spectrometer operation, specifications, and system compatibility. It also
includes accessory connector pinout diagrams and pin-specific information.
How the HR2000 Works
Below is a diagram of how light moves through the optical bench of an HR2000 Spectrometer. The optical bench
has no moving parts that can wear or break; all the components are fixed in place at the time of manufacture.
Items with an asterisk (*) are user-specified.
Figure 2-1: HR2000 Spectrometer with Components.
The HR2000 Component Table on the following page explains the function of each numbered component in the
HR2000 Spectrometer diagram (Figure 2-1).

HR2000 Specifications
HR2000 High-Resolution Fiber Optic Spectrometer 6
HR2000 Specifications Table
Ocean Optics permanently secures all components in the HR2000 at the time of manufacture. Only Ocean Optics
Technicians can replace interchangeable components, where noted.
Item Name Description
1 SMA Connector The SMA Connector secures the input fiber to the spectrometer. Light from the
input fiber enters the optical bench through this connector.
2 Slit*
The Slit is a dark piece of material containing a rectangular aperture, which is
mounted directly behind the SMA Connector. The size of the aperture regulates the
amount of light that enters the optical bench and controls spectral resolution.
You can also use the HR2000 without a Slit. In this configuration, the diameter of
the fiber connected to the HR2000 determines the size of the entrance aperture.
Only Ocean Optics technicians can change the Slit.
3 Filter*
The Filter is a device that restricts optical radiation to pre-determined wavelength
regions. Light passes through the Filter before entering the optical bench. Both
bandpass and longpass filters are available to restrict radiation to certain
wavelength regions.
Only Ocean Optics technicians can change the Filter.
4 Collimating Mirror
The Collimating Mirror focuses light entering the optical bench towards the Grating
of the spectrometer.
Light enters the spectrometer, passes through the SMA Connector, Slit, and Filter,
and then reflects off the Collimating Mirror onto the Grating.
5 Grating*
The Grating diffracts light from the Collimating Mirror and directs the diffracted light
onto the Focusing Mirror. Gratings are available in different groove densities,
allowing you to specify wavelength coverage and resolution in the spectrometer.
Only Ocean Optics technicians can change the Grating.
6 Focusing Mirror The Focusing Mirror receives light reflected from the Grating and focuses the light
onto the CCD Detector or L2 Detector Collection Lens (depending on the
spectrometer configuration).
7 L2 Detector
Collection Lens*
The L2 Detector Collection Lens (optional) attaches to the CCD Detector. It
focuses light from a tall slit onto the shorter CCD Detector elements.
The L2 Detector Collection Lens should be used with large diameter slits or in
applications with low light levels. It also improves efficiency by reducing the effects
of stray light.
Only Ocean Optics technicians can add or remove the L2 Detection Collection
Lens.
8 CCD Detector
(UV or VIS)
The CCD Detector collects the light received from the Focusing Mirror or L2
Detector Collection Lens and converts the optical signal to a digital signal.
Each pixel on the CCD Detector responds to the wavelength of light that strikes it,
creating a digital response. The spectrometer then transmits the digital signal to the
OOIBase32 application.

HR2000 Specifications
HR2000 High-Resolution Fiber Optic Spectrometer 7
HR2000 Specifications
The following sections provide specification information for the CCD detector in the HR2000, as well as the
HR2000 Spectrometer itself.
CCD Detector Specifications
Detector: Sony ILX511 linear silicon CCD array
No. of elements: 2048 pixels
Pixel size: 14 µm x 200 µm
Pixel well depth: 62,500 electrons
Signal-to-noise ratio: 250:1 (at full signal)
A/D resolution: 12 bit
Dark noise: 2.5 RMS counts
Corrected linearity: >99.8%
HR2000 Spectrometer Specifications
Dimensions: 148.6 mm x 104.8 mm x 45.1 mm
Weight: 570 g
Power consumption: 90 mA @ 5 VDC
Detector range: 200-1100 nm
Gratings: 13 gratings available
Entrance aperture: 5, 10, 25, 50, 100 or 200 µm wide slits or fiber (no slit)
Order-sorting filters: Installed longpass and bandpass filters
Focal length: f/4, 101 mm
Optical resolution: Depends on grating and size of entrance aperture
Stray light: <0.05% at 600 nm; <0.10% at 435 nm
Dynamic range: 2 x 108(system); 2000:1 for a single scan
Fiber optic connector: SMA 905 to single-strand optical fiber (0.22 NA)
Data transfer rate: Full scans into memory every 13 milliseconds
Integration time: 3 milliseconds to 65 seconds
Operating systems:
Windows 98/Me/2000/XP when using the USB interface on desktop or
notebook PCs
Any 32-bit Windows operating system when using the serial port on
desktop or notebook PCs

HR2000 Specifications
HR2000 High-Resolution Fiber Optic Spectrometer 8
System Compatibility
The following sections provide information on hardware and software requirements for the HR2000:
Compatibility for Desktop or Notebook PCs
To use the HR2000, you must have a PC that meets the following minimum requirements:
• IBM-compatible PC with Pentium (or higher) processor
• 32 MB RAM
• OOIBase32 Spectrometer Operating Software
• Windows 98/ME/2000/XP operating system (when connecting the HR2000 to a PC via USB port)
or
Any 32-bit version of Windows (when connecting the HR2000 to a PC via serial port)
Compatibility for Handheld PCs
To use the HR2000 with your handheld PC, the computer must meet the following minimum requirements:
• Handheld PC running Windows CE 2.11 or later
• 32 MB RAM
• OOIPS2000 Spectrometer Operating Software
• Serial port connectivity
20-Pin Accessory Connector Pinout
The HR2000 features a 20-pin Accessory Connector, located on the side of the unit as follows:
Figure 2-2: Location of HR2000 20-Pin Accessory Connector
20-Pin Connector

HR2000 Specifications
HR2000 High-Resolution Fiber Optic Spectrometer 9
20-Pin Accessory Connector Pinout Diagram
When facing the 20-pin Accessory Connector on the HR2000, pin numbering is as follows:
20 18 16 14 12 10 8 6 4 2
19 17 15 13 11 9 7 5 3 1
Figure 2-3: 20-Pin Accessory Connector Pinout Diagram
20-Pin Accessory Connector – Pin Definitions
The following table contains information regarding the function of each pin in the HR2000’s 20-Pin Accessory
Connector:
Pin # Description
1 VUSB or 5Vin
2 RS232 Tx
3 RS232 Rx
4 Lamp Enable
5 Continuous Strobe
6 Ground
7 External Trigger In
8 Single Strobe
9 I2C SCL
10 I2C SDA
11 No connect
12 No connect
13 No connect
14 No connect
15 No connect
16 No connect
17 No connect
18 No connect
19 No connect
20 No connect
20-Pin J2 Accessory Connector - Part Number and Compatibility
The part numbers for the 20-pin accessory connector on the HR2000 Spectrometer are as follows:
• The connector is Samtec model IPT1-110-01-S-D-RA.
• The mating right-angle connector is Samtec model IPS1-110-01-S-D-RA.
If you are customizing your HR2000 Spectrometer system or configuring External Triggering, you may need these
part numbers to complete your setup.

Installing the HR2000
HR2000 High-Resolution Fiber Optic Spectrometer 10
3 Installing the HR2000
This chapter contains instructions in parallel for connecting the HR2000 via both USB and serial modes.
Note: You must install the OOIBase32 software application prior to connecting the HR2000 Spectrometer to the
PC. The OOIBase32 software installation installs the drivers required for HR2000 installation. If you do
not install OOIBase32 first, the system will not properly recognize the HR2000.
If you have already connected the HR2000 to the PC prior to installing OOIBase32, consult Chapter 4:
Troubleshooting for information on correcting a corrupt HR2000 installation.
HR2000 Installation
USB Mode
To connect the HR2000 to a PC via the USB port, the
PC must be running the Windows 98/ME/2000/XP
operating system.
Note: The USB port on a PC can power up to five
HR2000 spectrometer channels. Systems with
more than five channels require a powered
USB hub.
Follow the steps below to connect the HR2000 to a
PC via the USB port:
1. Install OOIBase32 on the destination PC.
2. Locate the USB cable (USB-CBL-1) provided
with the HR2000.
3. Insert the square end of the cable into the
side of the HR2000.
4. Insert the rectangular end of the cable into the
USB port of the PC.
If you installed OOIBase32 prior to connecting the
HR2000, the Add New Hardware Wizard appears
and installs the HR2000 drivers. If the drivers do not
successfully install (or if you connected the HR2000 to
the PC before installing OOIBase32), consult Chapter
4: Troubleshooting.
Serial Port Mode
To use the serial port capacity of the HR2000
Spectrometer, the PC must be running a 32-bit
version of the Windows operating system (or Windows
CE 2.11 or higher for handheld PCs).
Follow the steps below to connect the HR2000 to the
PC via serial port:
1. Connect the serial cable adapter block to the
10 pins on the right side of the HR2000’s 20-
Pin Accessory Connector (pins 1-10).
2. Connect one end of the 9-pin serial cable to
the adapter block on the HR2000, and then
connect the other end to a serial port on the
PC.
3. Note the number of the serial port (COM Port)
to which you connected the HR2000 (some
PCs may not have numbered ports; handheld
PCs typically have only one serial port).
4. Plug the 5 VDC external power supply into an
outlet and connect it to the HR2000.
Note: Connecting the spectrometer to the PC’s
serial port requires that you manually enter
the calibration coefficients from the
Wavelength Calibration Data Sheet into
OOIBase32 software (select Spectrometer |
Configure | Wavelength Calibration tab).
See the OOIBase32 documentation for more
information.

Installing the HR2000
HR2000 High-Resolution Fiber Optic Spectrometer 11
Configuring the HR2000 in OOIBase32
Once you install the HR2000, you must configure OOIBase32’s Configure Spectrometer options so that
OOIBase32 recognizes the HR2000 Spectrometer.
Note: Consult the OOIBase32 Spectrometer Operating Software Manual for detailed instructions on configuring
the spectrometer in OOIBase32. You can find these instructions on the Software and Technical
Resources CD or on the Ocean Optics web site at the following location:
http://www.oceanoptics.com/technical/ooibase32.zip.
The following sections contain instructions on initially configuring the HR2000 the first time you start OOIBase32:
Operator and Serial Number Dialog Box
The Operator and Serial Number screen prompts you to enter a user name and software serial number into
OOIBase32. Some data files created by OOIBase32 during sampling procedures use this information in the file
headers.
Default Spectrometer Configuration File
The Default Spectrometer Configuration File screen prompts you to select a spectrometer configuration
(.SPEC) file for use with the HR2000. The unique serial number of the HR2000 precedes the file extension (for
example, HR2A0162.SPEC).
Navigate to the OOIBase32 installation directory and select the spectrometer configuration file.
Configure Hardware Screen
The Configure Hardware screen prompts you to enter spectrometer-specific information into OOIBase32 the first
time you run the program. Typically, you will only enter this information the first time you run OOIBase32.
However, you can alter the hardware configuration at any time using the Spectrometer Configuration screen.
Select Spectrometer | Configure from the OOIBase32 menu bar to access the Spectrometer Configuration
screen.
Follow the steps on the next page to configure the HR2000:
Note: You do not need to configure the spectrometer hardware in the OOIPS2000 handheld PC operating
software. Most handheld PCs have only one serial connector and do not have USB ports. Thus,
OOIPS2000 will communicate with the spectrometer via this port at a fixed baud rate. There is no way to
customize the HR2000 configuration with OOIPS2000.

Installing the HR2000
HR2000 High-Resolution Fiber Optic Spectrometer 12
Configure Hardware Screen - Continued
USB Mode
1. Specify S2000/PC2000/USB2000/HR2000 in
the Spectrometer Type drop-down menu.
2. Specify HR2000 in the A/D Converter Type
drop-down menu.
3. Specify the serial number of the HR2000
under the USB Serial Number drop-down
menu.
Note: The system pre-fills this drop-down
menu with the serial numbers of all
discovered HR2000 Spectrometers.
4. Click the OK button to accept the selected
options.
Serial Port Mode
1. Select the S2000/PC2000/USB2000/HR2000
option from the Spectrometer Type drop-
down menu.
2. Select the Serial (RS-232) A/D option from the
A/D Converter Type drop-down menu. This
selection enables serial-specific options in the
lower portion of the Configure Hardware
screen.
3. Select the COM port that the HR2000 is
connected to in the SAD500 Serial Port drop-
down menu. Consult Chapter 4:
Troubleshooting for information on identifying
serial ports.
4. Select the speed at which the HR2000 will
operate from the SAD500 Baud Rate drop-
down menu (115,200 baud is recommended).
5. Specify the pixel resolution (from 1 to 500) in
the SAD500 Pixel Resolution box. This value
specifies that every nth pixel of the
spectrometer will transmit from the HR2000 to
the PC.
Note: You can sacrifice pixel resolution to
gain speed. The transfer of one
complete spectra requires ~0.3
seconds at 115,200 baud.
6. Enable the Compress SAD500 Data
function to minimize the amount of data
transferred over the RS-232 connection.
The transmission of spectral data over the
serial port is a relatively slow process.
Enabling this function ensures that the
HR2000 compresses every scan that it
transmits. This greatly increases the data
transfer speed of the HR2000.
7. Click the OK button to complete setup.
The spectrometer should now be able to acquire data and respond to light. Exit and restart OOIBase32 to save
configuration data to disk.

Installing the HR2000
Spectrometer Configuration Screen
The Spectrometer Configuration screen prompts you to configure specific channel-level spectrometer information,
if necessary.
Select Spectrometer | Configure | Wavelength Calibration tab from the menu and set system parameters. If
you have connected your spectrometer to the PC’s USB port, OOIBase32 pre-fills the coefficients for the HR2000
from information on a memory chip in the spectrometer. Otherwise, you must manually type the coefficients as
they are printed on the Wavelength Calibration Data Sheet that accompanied your spectrometer. Verify that the
calibration coefficients match the coefficients from the Wavelength Calibration Data Sheet. If necessary, modify
these values using the USB Programmer utility. Additionally, ensure that you select both the Master and Channel
Enabled boxes.
In the A/D Interface tab, enter the same values as in the Configure Hardware dialog box. OOIBase32 stores this
information for future use once you close the program.
Note: For information on using the HR2000 with the OOIPS2000 Operating Software for the handheld PC, visit
http://www.oceanoptics.com/technical/palmspec.pdf.
Connect Spectroscopic Accessories
To find operating instructions for HR2000-compatible products (such as light sources, sampling chambers, and
probes), consult the Software and Technical Resources CD or the Ocean Optics web site at:
http://www.oceanoptics.com/technical/operatinginstructions.asp
External Triggering Options
You can trigger the HR2000 using a variety of External Triggering Options through the 20-pin Accessory
Connector on the spectrometer.
Consult the External Triggering Options document located at the following address:
http://www.oceanoptics.com/technical/externaltriggering.pdf
The External Triggering PDF contains instructions on configuring External Triggering Options for the HR2000.
Note: Only the external software triggering option is available when using a handheld PC.
HR2000 High-Resolution Fiber Optic Spectrometer 13

Troubleshooting
HR2000 High-Resolution Fiber Optic Spectrometer 14
4 Troubleshooting
The following sections contain information on troubleshooting issues you may encounter when using the HR2000
Spectrometer.
Note: For issues encountered when using a handheld PC, consult the OOIPS2000 manual.
HR2000 Connected to PC Prior to OOIBase32 Installation
If you connect your Ocean Optics USB device to the computer prior to installing your Ocean Optics software
application, you may encounter installation issues that you must correct before your Ocean Optics device will
operate properly.
Follow the applicable steps in this document to remove the incorrectly installed device, device driver, and
installation files.
Note: If these procedures do not correct your device driver problem, you will need to obtain the “Correcting
Device Driver Issues” document from the Ocean Optics website. Visit the following web address:
http://www.oceanoptics.com/technical/engineering/correctingdevicedriverissues.pdf
Remove the Unknown Device from Windows Device Manager
1. Open Windows Device Manager as follows:
Windows 98/ME:
• Go to the desktop and right-click on My Computer.
• Select Properties from the pop-up menu.
• Click on the Device Manager tab.
Windows 2000/XP:
• Click Start | Settings | Control Panel | System.
• Select the Hardware tab.
• Click on the Device Manager button.
2. Locate the Other Devices option and expand the Other Devices selection by clicking on the "+" sign to
the immediate left.
Note: Improperly installed USB devices can also appear under the Universal Serial Bus Controller
option. Be sure to check this location if you cannot locate the unknown device.
3. Locate the unknown device (marked with a large question mark). Right-click on the Unknown Device
listing and select the Uninstall or Remove option.

Troubleshooting
HR2000 High-Resolution Fiber Optic Spectrometer 15
4. Click the OK button to continue. A warning box appears confirming the removal of the Unknown Device.
Click the OK button to confirm the device removal.
5. Disconnect the HR2000 from your computer.
6. Locate the section in this chapter that is appropriate to your operating system and perform the steps in
the “Remove Improperly Installed Files” section.
Windows 98:
Remove Improperly Installed Files:
1. Open Windows Explorer.
2. Navigate to the Windows | INF directory. If the INF directory is not visible, you will need to disable the
“Hide System Files and Folders” option on in Windows Folder Options.
Note: If the INF directory is not visible, you will need to disable the “Hide System Files and Folders” and
“Hide File Extensions for Known File Types” options in Windows Folder Options.
You can access Windows Folder Options from Windows Explorer, under the View | Options
menu selection.
3. Delete the OOI_USB.INF file in the INF directory.
4. Navigate to the Windows | System32 | Drivers directory.
5. Delete the EZUSB.SYS file.
6. Reinstall your Ocean Optics application and reboot the system when prompted.
7. Plug in the USB device.
The system will now be able to locate and install the correct drivers for the USB device.

Troubleshooting
HR2000 High-Resolution Fiber Optic Spectrometer 16
Windows 2000/XP:
Remove Improperly Installed Files:
1. Open Windows Explorer.
2. Navigate to the Windows | INF directory. If the INF directory is not visible, you will need to disable the
“Hide System Files and Folders” option on in Windows Folder Options.
Note: If the INF directory is not visible, you will need to disable the “Hide System Files and Folders” and
“Hide File Extensions for Known File Types” options in Windows Folder Options.
You can access Windows Folder Options from Windows Explorer, under the Tools | Folder
Options menu selection.
3. Delete the OOI_USB.INF and OOI_USB.PNF files in the INF directory.
4. Navigate to the Windows | System32 | Drivers directory.
5. Delete the EZUSB.SYS file.
6. Reinstall your Ocean Optics application and reboot the system when prompted.
7. Plug in the USB device.
The system will now be able to locate and install the correct drivers for the USB device.