Hanna HI 5221 User manual

HI 5221 & HI 5222
pH/mV/ISE/Temperature
Bench Meters
Instruction Manual
www.hannainst.com
MAN5222 02/15
Printed in ROMANIA
  
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA
   
Tel. [phone]
Fax [phone]
E-mail [email protected]
www.hannainst.com
     

  and   are guaranteed for two years against defects in workmanship and materials when used
for their intended purpose and maintained according to instructions. Electrodes and probes are guaranteed for
six months. This warranty is limited to repair or replacement free of charge.
Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered.
If service is required, contact the dealer from whom you purchased the instrument. If under warranty, report
the model number, date of purchase, serial number and the nature of the problem. If the repair is not covered
by the warranty, you will be notified of the charges incurred. If the instrument is to be returned to Hanna
Instruments, first obtain a Returned Goods Authorization number from the Technical Service department and
then send it with shipping costs prepaid. When shipping any instrument, make sure it is properly packed for
complete protection.
To validate your warranty, fill out and return the enclosed warranty card within 14 days from the date of
purchase.
Dear Customer,
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using these instruments. This manual will provide you
with the necessary information for correct use of these instruments, as well as a precise idea of their
versatility.
If you need additional technical information, do not hesitate to e-mail us at [email protected] or view our
worldwide contact list at www.hannainst.com.
WARRANTY
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner.
OTHER ACCESSORIES
  Voltage adapter from 115 Vac / 12 Vdc 800 mA (USA plug)
  Voltage adapter from 230 Vac / 12 Vdc 800 mA (European plug)
  Electrode holder
  pH and ORP electrode simulator with 1 m (3.3') coaxial cable ending in female BNC
connectors
  pH and ORP electrode simulator with LCD and 1 m (3.3') coaxial cable ending in female BNC
connectors
  Temperature probe with 1 m (3.3') cable
  Windows®compatible software
  USB cable
RECOMMENDATIONS FOR USERS
Before using these products, make sure they are entirely suitable for the environment in which they are used.
Operation of these instruments in residential areas could cause unacceptable interferences to radio and TV
equipment, requiring the operator to follow all necessary steps to correct interferences.
The glass bulb at the end of the pH electrode is sensitive to electrostatic discharges. Avoid touching this glass
bulb at all times.
Any variation introduced by the user to the supplied equipment may degrade the instruments’ EMC
performance.
To avoid electrical shock, do not use these instruments when voltages at the measurement surface exceed 24 Vac
or 60 Vdc.
To avoid damage or burns, do not perform any measurement in microwave ovens.
Hanna Instruments reserves the right to modify the design, construction or appearance of it’s products without
advance notice.

3
TABLE OF CONTENTS
WARRANTY.............................................................................................................................................2
PRELIMINARY EXAMINATION.................................................................................................................... 3
GENERAL DESCRIPTION ...........................................................................................................................4
FUNCTIONAL DESCRIPTION...................................................................................................................... 5
SPECIFICATIONS ...................................................................................................................................... 8
OPERATIONAL GUIDE ...............................................................................................................................9
DISPLAYING MODES .............................................................................................................................. 11
SYSTEM SETUP ..................................................................................................................................... 14
pH SETUP ............................................................................................................................................ 21
mV SETUP ............................................................................................................................................ 34
ISE SETUP (  only).................................................................................................................... 36
pH CALIBRATION...................................................................................................................................44
pH MEASUREMENT ............................................................................................................................... 47
mV & Relative mV MEASUREMENTS ......................................................................................................49
ISE CALIBRATION (  only)..........................................................................................................51
ISE MEASUREMENT (  only)...................................................................................................... 54
TEMPERATURE CALIBRATION ................................................................................................................. 60
LOGGING.............................................................................................................................................. 61
PC INTERFACE ...................................................................................................................................... 64
ADDITIONAL INFORMATION ................................................................................................................... 65
pH BUFFER TEMPERATURE DEPENDENCE............................................................................................... 68
ELECTRODE CONDITIONING AND MAINTENANCE ..................................................................................... 69
TROUBLESHOOTING GUIDE ................................................................................................................... 71
TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS ..........................................................................72
ACCESSORIES ........................................................................................................................................ 73
Remove the instrument from the packing material and examine it carefully to make sure that no damage has
occurred during shipping. If there is any damage, notify your Dealer or the nearest Hanna Customer Service
Center.
PRELIMINARY EXAMINATION
78
CONNECT TO
SCREW TYPE
ELECTRODES
CONNECT TO THE
BNC SOCKET
OF THE METER
HI 7855 SERIES CABLE CONNECTORS
CONNECTOR AND 3.0 mm (0.12") CABLE WITH BNC
ORP ELECTRODES

Glass body, refillable, combination platinum  electrode.
Use: titration.

Plastic body (PEI), gel filled, combination platinum  electrode.
Use: general purpose.

Plastic body (PEI), gel filled, combination gold  electrode.
Use: general purpose.
Consult the Hanna General Catalog for more electrodes with screw-type or BNC connectors.
EXTENSION CABLE FOR SCREW-TYPE ELECTRODES
(SCREW TO BNC ADAPTER)
  Extension cable 1 m (3.3') long
  Extension cable 3 m (9.9') long

4
GENERAL DESCRIPTION
  and   are professional bench meters with a color graphic LCD for pH (with Calibration Check), ORP
(Oxidation Reduction Potential), ISE (  only) and Temperature measurements.
The display can be configured as a single channel or dual channel display (  only). Each channel can be
configured as pH, mV, Relative mV or ISE (  only).
The main features of the instruments are:
•Single ( ) or two ( ) input channels;
•Capacitive touch keypad;
•pH calibration using up to five pH buffers: choose from the list of Hanna buffers (1.68, 3.00, 4.01, 6.86,
7.01, 9.18, 10.01 and 12.45) or custom buffers (up to 5 custom buffers);
•ISE calibration using up to five standards: choose from the list of nominal standard values (e.g. for ppm:
0.010, 0.100, 1.00, 10.0, 100, 1000, 10000 ppm) and/or from 5 user-supplied custom standard values
(up to 5 custom solutions);
•AutoHold feature to freeze first stable reading on the LCD;
•Two selectable alarm limits;
•User Selectable logging modes: Automatic logging, Manual logging with or without AutoHold;
•Continuous Lot logging directly on meter, with selectable log interval: Store up to 100,000 total data points;
•Up to 100 logging lots; a single lot can have up to 50,000 data points;   (same for each measurement
channel). Log files include GLP information;
•Basic Measurement can be viewed with detailed GLP information, or with a Graph or a Log History;
•User-friendly interface on large color graphic LCD (240 x 320 pixels) with user selectable color palette;
•PC interface via USB; download logged data to PC or use for Real time logging (  PC application
required);
The meters are supplied complete with:
•  Glass body Combination pH Electrode
•  Temperature probe
•  Electrolyte Solution
•  Electrode Holder
•pH Calibration Solutions Kit
•One capillary dropper pipette
•12 Vdc Power Adapter
•Instruction Manual
  and   are supplied with 12 Vdc/115 Vac adapter.
  and   are supplied with 12 Vdc/230 Vac adapter.
 Save all packing material until you are sure that the instrument works properly. Any defective item
must be returned in the original packing with the supplied accessories.
77

Plastic body (PVDF), open junction, conical, Viscolene, non refillable, combination  electrode.
Use: meat & cheese.

Glass body, double junction, conical, Viscolene, non refillable, combination  electrode.
Use: milk, yogurt.

Glass body, triple ceramic, single junction, refillable, combination  electrode.
Use: food processing.

Plastic body (PVDF), double junction, refillable with built-in amplifier, combination  electrode.
Use: very high humidity.

5
HI 5221 DESCRIPTION
 
1) Liquid Crystal Display (LCD)
2) Capacitive touch keypad
3) ON/OFF switch
4) Power adapter socket
5) USB connector
6) BNC electrode connector for pH/ORP measurements
7) Temperature probe socket
8) Reference input socket
FUNCTIONAL DESCRIPTION
 
•Dedicated Help key with contextual message;
•Profile feature: store up to ten different user setup (  - five on each channel).
76

Glass body, semimicro, conical, refillable, combination  electrode.
Use: semisolid products.

Plastic body (PEI), double junction, refillable, combination  electrode.
Use: general purpose.

Glass body, single junction, flat tip, Viscolene, non refillable, combination  electrode.
Use: surface measurement.

Plastic body (PVDF), double junction, refillable, combination  electrode.
Use: general purpose for food industry.

6
HI 5222 DESCRIPTION
 
1) Liquid Crystal Display (LCD)
2) Capacitive touch keypad
3) ON/OFF switch
4) Power adapter socket
5) USB connector
6) BNC electrode connector for pH/ORP/ISE measurements (Channel 1)
7) Temperature probe socket (Channel 1)
8) Reference input socket (Channel 1)
9) Reference input socket (Channel 2)
10) Temperature probe socket (Channel 2)
11) BNC electrode connector for pH/ORP/ISE measurements (Channel 2)
 
75

Glass body, refillable, double junction, combination  electrode.
Use: general purpose.

Glass body, semimicro, single junction, refillable, combination  electrode.
Use: laboratory, vials.

Glass body, semimicro, single junction, refillable, combination  electrode.
Use: flasks.

Plastic body (PEI), double junction, gel filled, combination  electrode.
Use: general, field.

7
KEYBOARD DESCRIPTION
 
 
The upper row keys are assigned to the   placed on the bottom of the LCD, which allow you to
perform the displayed function, depending on the current menu (e.g. ,
and in
Measure mode).
:All the virtual keys are assigned to the highlighted channel (  only).
LCD GENERAL DESCRIPTION
To enter/exit calibration mode.
To select the desired measurement mode, pH, mV, Rel mV (or ISE –   only).
To enter Setup (System, pH, mV or ISE) and to access Log Recall function.
To obtain general information about the selected option/operation.
74
ORP SOLUTIONS
 Test Solution 200-275 mV, 500 mL bottle
 Test Solution 240 mV, 500 mL bottle
 Test Solution 470 mV, 500 mL bottle
 Reducing Pretreatment Solution, 500 mL
 Oxidizing Pretreatment Solution, 500 mL
pH ELECTRODES
All electrodes part numbers ending in B are supplied with a BNC connector and 1 m (3.3') cable, as shown
below:

Glass body, double junction, refillable, combination  electrode.
Use: strong acid/alkali.

Glass body, triple ceramic, conical shape, refillable, combination  electrode.
Use: emulsions.

Glass body, micro, Viscolene, non refillable, combination  electrode.
Use: biotechnology, micro titration.

8
SPECIFICATIONS
 
egnaR
Hp000.02ot000.2-/Hp00.02ot00.2-/Hp0.02ot0.2-
Vm0.0002±
-01.g.e
7-
01ot 01ot500.0,M
5
,mpp
01·5
7–
01·5ot
7
.cnoc
K51.393ot51.352/F°0.842ot0.4-/C°0.021ot0.02-
noituloseR
Hp100.0/Hp10.0/Hp1.0
Vm1.0
- stigidstnacifingis3,2,1
K1.0/F°1.0/C°1.0
ycaruccA
F°77/C°52@
tigidtnacifingistsal1±Hp200.0±/Hp10.0±/Hp1.0±
tigidtnacifingistsal1±Vm2.0±
-)snoitnelavonom(%5.0±
)snoitnelavid(%1±
K2.0±/F°4.0±/C°2.0±
egnartesffoVmevitaleR Vm0.0002±
noitarbilaCHp elbaliavasreffubdradnats8,noitarbilacstniopevifotpU
sreffubmotsuc5dna,)54.21,10.01,81.9,10.7,68.6,10.4,00.3,86.1(
noitarbilaCESI -
,noitarbilacstniopevifotpU
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,tinutnemerusaemhcaerof
snoitulosmotsucevifdna
noitarbilaCresUerutarepmeT 001,05,0(stniop3 C° )
slennahCtupnI Vm/Hp1 ESI/Vm/Hp2
noitasnepmoCerutarepmeT citamotuArolaunaM
PLG pmatsemitnoitarbilac,stniopnoitarbilac,epols/tesffoedortcelE
droceRgniggoL lennahc/stniopatad000,001mumixam/tol/xamsdrocer000,05,stol001otpU
lavretnIgniggoL setunim081dnadnoces1neewtebelbatceles41
epyTgniggoL dloHotuAdnadnamednogoL,citamotuA
edortcelEHp 
eborPerutarepmeT 
ecafretnICP BSUdetalosi-otpO
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yalpsiD )slexip023x042(DCLcihparGroloC
ylppuSrewoP retpadacdV21
tnemorivnE gnisnednoc-nonHR%59.xamK323-372/F°221-23/C°05-0
snoisnemiD )"7.3x1.9x3.6(mm49x132x061
thgieW ).bl6.2(gK2.1
73
ACCESSORIES
pH BUFFER SOLUTIONS
  pH 1.679 Buffer Solution, 500 mL bottle
  pH 3.000 Buffer Solution, 500 mL bottle
  pH 4.01 Buffer Solution in FDA approved bottle, 500 mL
  pH 4.010 Buffer Solution, 500 mL bottle
  pH 6.86 Buffer Solution in FDA approved bottle, 500 mL
  pH 6.862 Buffer Solution, 500 mL bottle
  pH 7.01 Buffer Solution in FDA approved bottle, 500 mL
  pH 7.010 Buffer Solution, 500 mL bottle
  pH 9.18 Buffer Solution in FDA approved bottle, 500 mL
  pH 10.01 Buffer Solution in FDA approved bottle, 500 mL
  pH 10.010 Buffer Solution, 500 mL bottle
  pH 12.450 Buffer Solution, 500 mL bottle
ELECTRODE STORAGE SOLUTIONS
  Storage Solution, 500 mL bottle
  Storage Solution in FDA approved bottle, 500 mL
ELECTRODE CLEANING SOLUTIONS
  Electrode Rinse Sachets, 20 mL, 25 pcs
  General Purpose Solution, 500 mL bottle
  Protein Cleaning Solution, 500 mL bottle
  Inorganic Cleaning Solution, 500 mL bottle
  Oil & Fat Cleaning Solution, 500 mL bottle
  General Purpose Solution in FDA approved bottle, 500 mL
  Protein Cleaning Solution in FDA approved bottle, 500 mL
  Oil & Fat Cleaning Solution in FDA approved bottle, 500 mL
ELECTRODE REFILL ELECTROLYTE SOLUTIONS
  3.5M KCl + AgCl Electrolyte, 4x30 mL, for single junction electrodes
  1M KNO3Electrolyte, 4x30 mL
  3.5M KCl Electrolyte, 4x30 mL, for double junction electrodes
  3.5M KCl + AgCl Electrolyte in FDA approved bottle, 4x30 mL, for single junction electrodes
  1M KNO3Electrolyte in FDA approved bottle, 4x30 mL
  3.5M KCl Electrolyte in FDA approved bottle, 4x30 mL, for double junction electrodes
  1M KCl + AgCl Electrolyte in FDA approved bottle, 4x30 mL

9
OPERATIONAL GUIDE
POWER CONNECTION
Plug the 12 Vdc adapter into the power supply socket.
 •These instruments use non-volatile memory to retain the pH, ISE calibrations and all other
settings, even when unplugged.
•Make sure a fuse protects the main line.
ELECTRODE AND PROBE CONNECTIONS
For pH or ORP measurements, connect a pH/ORP electrode with internal reference to the BNC connector
located on the rear panel of the instrument (to the desired channel -   only).
For ISE measurements, (  only) connect, to the desired channel, an ISE electrode with internal
reference to the BNC connector located on the rear panel of the instrument.
For electrodes with a separate reference, connect the electrode’s BNC to the BNC connector and the electrode’s
reference to the reference input socket.
For temperature measurement and automatic temperature compensation, connect the temperature probe to the
appropriate socket (to desired channel -   only).
INSTRUMENT START UP
•Please ensure that the capacitive keypad is not covered by hand or other objects at the meter power on.
•Turn the instrument on from the power button located on the rear panel of the instrument.
•Please wait until the instrument finishes the initialization process.
 It is normal for the loading process to take a few seconds. If the instrument doesn’t display the next
screen, restart the meter using the power button. If the problem persists, contact your dealer.
72
TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS
The resistance of glass electrodes partially depends on the temperature. The lower the temperature, the
higher the resistance. It takes more time for the reading to stabilize if the resistance is higher.
Since the resistance of the pH electrode is in the range of 50 – 200 Mohm, the current across the
membrane is in the pico Ampere range. Large currents can disturb the calibration of the electrode for many
hours.
The pH electrode’s life also depends on the temperature. If constantly used at high temperatures, the
electrode life is drastically reduced.
  
Ambient Temperature 1 – 3 years
90 °C (194 °F) Less than 4 months
120 °C (248 °F) Less than 1 month
 
High concentrations of sodium ions interfere with readings in alkaline solutions. The pH at which the
interference starts to be significant depends upon the composition of the glass. This interference is called
alkaline error and causes the pH to be underestimated.

10
CHANNEL SELECTION (HI 5222 only)
•Press while in
Measure mode to access channel
selection menu. Four available options will be displayed:
Channel 1, Channel 2, or multi-channel with the first or
the second channel focused. The “ 
” message is displayed in the Reminder
messages area.
•Select the desired option by pressing the appropriate key:
, , or . The instrument
will display in the selected option Measure mode.
71
TROUBLESHOOTING GUIDE
  
evissecxe/esnopserwolS
.tfird
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.noitcnujecnereferytriD
nipitedortceleehtkaoS 
nehtdnasetunim03rofnoitulos
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11
DISPLAYING MODES
For each measurement mode (pH, mV, Rel mV or ISE) the following display configurations are available:
Basic, Good Laboratory Practice (GLP) (pH, ISE only), Graph and Log History.

The main measured value and it’s units are displayed on the LCD, along with the temperature value,
temperature probe status and basic calibration information when this option is accessed.
To choose the Basic display mode:
•Press while in
Measure mode. The “  ” message will be
displayed in the Reminder messages area.
•Press . The instrument will display the basic information for the selected measurement mode.

Detailed GLP data will be displayed on the LCD for pH Measure and ISE Measure modes only when this
option is selected: Last Calibration date and time, Offset and Slope values, Calibration Buffers/Standards and
general information regarding the buffers/standards: the calibration temperature, temperature compensation
mode, date and time. For
pH Measure , the Electrode Condition is also displayed on the LCD in percent.
:If a one point pH calibration is performed or the current calibration does not include at least two
consecutive standard buffers of pH 4.01, 7.01 (6.86) and 10.01 (9.18) buffers, the Electrode Condition
will be unknown. Electrode condition remains active for 24 hours after a calibration.
To access the GLP display option:
•Press while in
Measure mode. The “  ” message will be
displayed in the Reminder messages area.
•Press . The instrument will display the detailed GLP data.
70
 
If the electrode does not respond to pH changes, the battery may have run down and the electrode or battery
(if replaceable) should be replaced.
MEASURE
Rinse the pH electrode tip with distilled water. Immerse the tip bottom 4 cm (1.5”) in the sample and stir
gently for a few seconds.
For a faster response and to avoid cross-contamination of the samples, rinse the electrode tip with a few drops
of the solution to be tested, before taking measurements.
STORAGE PROCEDURE
To minimize clogging and ensure a quick response time, the glass bulb and the junction of pH electrode
should be kept moist and not allowed to dry out.
Replace the solution in the protective cap with a few drops of   or   Storage Solution or,
in its absence, Filling Solution (  or   for single junction and   or   for double
junction electrodes). Follow the Preparation Procedure before taking measurements.
 NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.
PERIODIC MAINTENANCE
Inspect the electrode and the cable. The cable used for connection to the instrument must be intact and there
must be no points of broken insulation on the cable or cracks on the electrode stem or bulb. Connectors must
be perfectly clean and dry. If any scratches or cracks are present, replace the electrode. Rinse off any salt
deposits with water.
pH PROBE MAINTENANCE
  
Refill the reference chamber with fresh electrolyte (  or   for single junction or   or
  for double junction electrodes). Allow the electrode to stand upright for 1 hour. Follow the Storage
Procedure above.
pH CLEANING PROCEDURE
•
General Soak in Hanna   or   General Cleaning Solution for approximately 30 minutes.
•
Protein Soak in Hanna   Protein Cleaning Solution for 15 minutes.
•
Inorganic Soak in Hanna   Inorganic Cleaning Solution for 15 minutes. This is good at
cleaning a black ceramic junction.
•
Oil/grease Rinse with Hanna   or   Oil and Fat Cleaning Solution.
 After performing any of the cleaning procedures, rinse the electrode thoroughly with distilled
water, refill the reference chamber with fresh electrolyte (not necessary for gel-filled electrodes) and soak the
electrode in   or   Storage Solution for at least 1 hour before taking measurements.

12

The on-line graph with currently logged values (pH, mV, Rel mV, or ISE vs. Seconds) will be displayed when
this option is selected.
If there is no active log, the previously logged data for the selected parameter will be shown.
To access the off-line / on-line graph:
•Press while in
Measure / Logging mode. The “  ” message will
be displayed in the Reminder messages area.
•Press . Press to begin an online graph.
  
•Press then . and will appear in virtual keys.
•Use and to move along the X (Time) axis of the graph.
•Press to access the zoom menu for Y axis. Use or for zooming Y (parameter) axis.
•Press to return to the main menu.
69
PREPARATION PROCEDURE
Remove the protective cap off the pH electrode.
SALT DEPOSITS MAY BE PRESENT. This is normal with electrodes. They will disappear when rinsed with water.
During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of the
electrode. These bubbles can be removed by “shaking down” the electrode as you would do with a glass
thermometer.
If the bulb and/or junction is dry, soak the electrode in   or   Storage Solution for at least
one hour.
  
If the filling solution (electrolyte) is more than 2½ cm (1”) below the fill hole, add   or  
3.5M KCl Electrolyte Solution for double junction or   or   3.5M KCl+AgCl Electrolyte
Solution for single junction electrodes.
Unscrew the fill hole screw during measurements. This will allow electrolyte to flow out of the junction.
ELECTRODE CONDITIONING AND MAINTENANCE

13
When the   is displayed:
•Use the arrow keys to move along the X (Time) and Y (parameter) axes of the graph.
•Press to access the zoom menu for X and Y axes. Use or / /
/ to switch between the active zooming axes. Press or to zoom the selected axis.
:While in zoom graph menu the key is not accessible.
•Press to return to the main menu.
 
The measurement, along with  , will be visible when this option is selected:
1) The last stored logged data (Not actively logging) or
2) The last data logged from an active logging lot or
3) An empty display - NO LOTS saved, NOT currently logging
The log history list also contains the appropriate mV values, the logged temperature, the temperature probe
status source, as well as the records time stamp.
To access the Log History display option:
•Press while in
Measure mode. The “  ” message will be
displayed in the Reminder messages area.
•Press . The instrument will display the log history regarding the selected Measure mode.
 •When an alarm condition is active, all logged records will have an exclamation mark “”.
•If logged in Auto Hold, logged records will have an “”.
•If another
Measure mode is selected, the Log History will reset.
•If the temperature unit is changed, all logged temperature values will be automatically displayed
in the new temperature unit.
68
pH BUFFER TEMPERATURE DEPENDENCE
Temperature has an effect on pH. The calibration buffer solutions are affected by temperature changes to a
lower degree than normal solutions.
During calibration the instrument will automatically calibrate to the pH value corresponding to the measured
or set temperature.
During calibration the instrument will display the pH buffer value at 25 °C.
PMET SREFFUBHp
C° K° F° 976.1 000.3 010.4 268.6 010.7 771.9 010.01 454.21
0 372 23        
5 872 14        
01 382 05        
51 882 95        
02 392 86        
52 892 77        
03 303 68        
53 803 59        
04 313 401        
54 813 311        
05 323 221        
55 823 131        
06 333 041        
56 833 941        
07 343 851        
57 843 761        
08 353 671        
58 853 581        
09 363 491        
59 863 302        

14
SYSTEM SETUP
The System Setup menu allows the user to customize the user interface, view meter information, set the
external serial communication interface and to restore the manufacturer settings.
  
•Press while in Measure mode.
•Press . The system setup options will be displayed on
the LCD.
To access a System Setup option:
•Use or to highlight the desired option.
•Press to access the selected option.
The following is a detailed description of the System Setup option screen:
Beeper
This option allows the user to turn an acoustic warning signal on
or off. This function can be used to signal 4 different events: a
stable signal, an alarm state, when every key is pressed or when
an incorrect key is pressed. Enable (or disable) the Beeper for
these events. Disabling the Beeper will stop audible signals.
67
∆E-the difference of potential from the electrode;
S-the electrode slope, determined in a previous
calibration;
f-the stoichiometric ratio between sample and
standard;
where: CSAMP -the sample concentration;
CSTD -the standard concentration;
VSAMP -the sample volume;
VSTD -the standard volume;
VISA -ISA volume
where: CSAMP -the sample concentration;
CSTD -the standard concentration;
VSAMP -the sample volume;
VSTD -the standard volume;
VISA -ISA volume;
∆E-the difference of potential from the electrode;
S-the electrode slope, determined in a previous
calibration;
f-the stoichiometric ratio between sample and
standard;
Example 1
You have sulfide samples and you are adding
Ag+ . The reaction is:
One mole sulfide sample reacts with 2 moles silver standard (
f = 1/2).
Example 2
You have sulfide samples and you are adding
Pb2+ . The reaction is:
One mole sulfide sample reacts with 1 mole lead standard (
f = 1).
    
Analyte Addition and Subtraction are variations of the previous two methods.
With Analyte Addition,
sample (analyte) is added to an Ion standard being measured. The standard and
sample contain the same Ion. mV are taken before and after the sample addition. From the mV the analyte
concentration is determined.
With Analyte Subtraction,
sample (analyte) is added to an Ion standard being measured. The analyte reacts
with the measured Ion in a known manner thus removing measured ions from the solution. From the change
in mV the concentration of the analyte is determined.
S2- + 2Ag+ →Ag2S
S2- + Pb2+ →PbS
CSAMP = CSTD ·VSTD · (VSTD + VSAMP + VISA)·10
∆ E
- (VSTD + VISA)
(VSTD + VISA) VSAMP
S
CSAMP = f ·{(VSTD + VISA)- [1 + (VSTD + VISA)]·10
∆ E}·(CSTD·VSTD )
VSAMP VSAMP VSTD + VISA
S

15
Saving Confirmation
Enable this option to force confirmation of a change made to a
setting in GLP data option field or a Sample ID name. If Saving
Confirmation is enabled, the user will have to accept the change
with a key stroke. If Saving Confirmation is disabled, the changes
made to these fields change automatically without asking for
confirmation.
GLP Data
Use this option to customize logging GLP information with specific identification data. When enabled, these ID
tags will be included in the GLP section of all data logs for all modes of operation and both channels ( ).
Each data field can use up to 10 characters.
The five available fields are:
  : used to add the name of the operator
  : used to name an instrument with a discrete name, location or number
  : used to include the Company ID to the GLP data field.
  : Two data fields are available for general notes or notations.
66
The Nernst equation can be rewritten:
ION SELECTIVE ANALYSIS METHODS
 
This method is a simple procedure for measuring multiple samples. It should only be used in the linear
working regions of the sensor. A direct reading instrument such as the   determines concentration of
the unknown by a direct reading after calibrating the instrument with the standards. The instrument is
calibrated as described in “ISE CALIBRATION & MEASUREMENTS” section, with two or more freshly made
standards that are in the measurement range of the unknowns. Ionic strength adjustment is made to samples
and standards. Unknowns are measured directly by the instrument.
At lower concentrations, in non-linear regions of the electrode response, multiple calibration points will extend
measurements to a practical detection limit. Calibrations must be performed more frequently in these cases.
 
Incremental methods are useful for the measurement of samples whose constituents are variable or
concentrated. Incremental techniques can reduce errors from such variables as temperature, viscosity, or pH
extremes and will provide indirect analysis of ions for which there is no ISE sensor for a direct measurement.
There are four commonly used different incremental methods for sample measurement. They are Known
Addition, Known Subtraction, Analyte Addition and Analyte Subtraction.   allows the analyst to use
these techniques as a simple routine procedure, thus eliminating calculations or tables. The method once set
up can be used for repetitive measurements on multiple samples.
    
With Known addition,
standard is added to a sample being measured. The standard and sample contain the
same Ion. mV are taken before and after the standard addition. From the change in mV, the sample
concentration is determined.
With Known subtraction,
a known standard is added to an ionic sample being measured. The standard reacts
with the measured Ion in the sample in a known manner, thus removing measured ions from the solution.
From the change in mV, the concentration of the sample is determined.
E= Eo+ S log(C)
CSAMP = CSTD ·VSTD ·f · (VSAMP + VISA)
(VSAMP + VISA)- (VSAMP + VSTD + VISA)·10
∆ EVSAMP
S
CSAMP = CSAMP ·VSTD · (VSAMP + VISA)
(VSAMP + VSTD + VISA)·10
∆ E- (VSAMP + VISA) VSAMP
S

16
To add the GLP Data:
•Press while in
Measure mode.
•Press .
•Use or to select the GLP Data option.
•Press and use or to highlight the
desired option.
•Press to edit the desired information. The Text Editor
menu will be displayed on the LCD.
•Enter the desired information by accepting the highlighted
character which is added to the text bar, using . The
and keys help the user to select the desired character. It is also possible to delete the last
character by positioning the cursor on the Backspace character ( ) and pressing .
•Press to return to the GLP Data options. If the Saving Confirmation is enabled, press to
accept the modified option, to escape without saving or to return to the editing mode.
Otherwise, the modified options are saved automatically.
  
Set the current date & time and the format in which they appear.
Set Date and Time
This option allows you to set the current date (year/month/day) and time (hour/minute/second).
 •Only years starting with 2000 are accepted.
•The time is set using the selected time format. For 12 Hour time format only, the AM/PM can
also be selected with or .
  
Choose between 12-Hour (AM/PM) time format or 24-Hour time format.
  
Choose the desired date format from 7 available options: DD/MM/YYYY,MM/DD/YYYY,YYYY/MM/DD,YYYY-MM-DD,
Mon DD, YYYY, DD-Mon-YYYY or YYYY-Mon-DD.
To set the Date & Time:
•Press while in
Measure mode.
•Press .
65
ADDITIONAL INFORMATION
ISE THEORY
An Ion Selective Electrode (ISE) is an electrochemical sensor that changes voltage with the activity or concentration
of ions in solutions. The change in voltage is a logarithmic relationship with concentration, and is expressed by
the Nernst equation:
where: E- the measured voltage;
Eo- standard voltage and other standard system voltages;
a- the activity of the Ion being measured;
S- the Nernst slope factor and is derived from thermodynamic principles:
R- the universal gas constant (8.314 J/Kmol);
T- the temperature in degrees Kelvin;
F- the Faraday’s constant (96,485 C/mol);
n- the Ion charge.
The slope may be positive or negative depending upon the Ion charge (n).
Activity and concentration are related by an “activity coefficient”, expressed as:
where: a- the activity of the Ion being measured;
γ - the activity coefficient;
C- the concentration of the Ion being measured.
In very dilute solutions γ approaches 1 so activity and concentration are the same.
Actual samples that are more concentrated have much smaller activity coefficients (γ < 1). The addition of an
inert background salt to standards and samples stabilizes the activity coefficient so that concentration measurements
may be made directly. Some of Hanna’s Ionic Strength Adjuster formulations also may optimize pH and complex
interferences, in addition to standardizing the ionic strength.
 

noitactnelavonoM 61.95+
noinatnelavonoM 61.95–
noitactnelaviD 85.92+
noinatnelaviD 85.92–
E= Eo+ S log(a)
S= 2.303RT
nF
a= γ ·C

17
•Use or to select the Date & Time option.
•Press and use or to highlight the
desired option you want to modify.
•Press to confirm your selection. Use /
to select next/previous entry to be edited. Press and
use or to set the desired value, then press
to save the modified value (for Set Date and Time
option). For the Set Date Format option press to confirm
your selection and select one of the displayed options with
or .
•Press to confirm your selection and return to the Date & Time options or press to cancel
operation.
:If the time is changed with more than one hour before last pH/ISE calibration, a pop-up warning
will appear on the LCD, notifying the user that a date/time conflict has occurred and some
time-dependent modes could work improperly (e.g.
Measure , GLP, Log).
LCD Setup
This option allows the user to set the
Contrast , the Backlight of the LCD and the Backlight Saver . The Contrast
parameter can be adjusted within 7 steps, while the Backlight parameter within 8 steps. The Backlight Saver can
be set from 1 to 60 minutes or it can be OFF (disabled). All the changes are visible on the LCD for each parameter.
:If the instrument backlight turns off after the set period of time, press any key to turn it back on.
To set the LCD Setup:
•Press while in
Measure mode.
•Press .
•Use or to select the LCD Setup option.
•Press and use key to highlight the desired
parameter.
•Use or to adjust the contrast / backlight or to
set the desired backlight saver time.
•Press to confirm the modified options and return to the
System Setup menu.
64
PC INTERFACE
Data transmission from the instrument to the PC can be done with the   Windows®compatible
software (optional).   also offers graphing and on-line help features.
Data logged on the   and   meters can be exported to the most popular spreadsheet programs
for further analysis.
  and   instruments have an USB interface available.
Use a standard USB cable to connect your instrument to the PC.
Make sure that the instrument and the   software have the same baud rate and the appropriate
communication port.
The PC software may also be used for real time logging.
  :
•Press while in
Log Recall mode.
•Press or to access delete or delete all
mode. Otherwise, press to return to Log Recall view
mode.
•After selecting one of the deleting modes, use or
to select one lot and then press or
to delete the selected lot or all lots. The “ ”
message will be displayed on the LCD until the selected lot or
all lots are deleted.
•Press and then press to exit deleting mode
and return to Log Recall view mode.
•Press to exit
Log Recall mode and return to Measure mode.
:Logged lots should also be deleted whenever “    ” or “  
” message appears on the LCD, in the Reminder messages area.

18
Color Palette
This option allow the user to choose desired color palette.
To select the Color Palette:
•Press while in
Measure mode.
•Press .
•Use or to select the Color Palette option.
•Press and use or to highlight the
desired color.
•Press to confirm your selection and return to the System
Setup menu or press to return to the System Setup menu
without changing.
Language
This option allows the user to choose the desired language in which all information will be displayed.
To select the Language:
•Press while in
Measure mode.
•Press .
•Use or to select the Language option.
•Press and use or to highlight the
desired language.
•Press to confirm your selection and return to the System
Setup menu or press to return to the System Setup menu
without changing.
63
•Press , or to select the desired Log
Report type. All logged lots for the selected Log Report type
will be displayed on the LCD.
•To filter the displayed lots, press and then the
desired parameter , or -  
only. Only the selected measurement parameter lots will be
displayed on the LCD.
•Select the desired lot with or and press
to display the logged data from the highlighted lot.
The “ ” message will be displayed on the LCD
for one second. The selected Logging Data Configuration
options will be displayed on the LCD, together with GLP
information (last calibration date and calibrated buffers/
standards) if a calibration has been performed on the selected
mode and the logged values (measured value, mV value,
temperature value, temperature compensation mode and the
logging time).
:For automatic logging only, it is possible to view the
plotted graph.
•Press to display the graph.
•By pressing it is possible to move the graph
along the X or Y axis with the arrow keys.
•If pressing while the graph is displayed, the
zoom menu for the X and Y axes will be accessed.
Press or / / /
to switch between the active zooming axes
and then zoom in or out on the selected axis by
pressing the appropriate virtual key.
•Press to return to the previous menu at any time.

19
Serial Communication
This option allows the user to set the desired speed for the serial communication (baud rate) in bps. The meter and
the PC program must have the same baud rate.
To set the Serial Communication:
•Press while in
Measure mode.
•Press .
•Use or to select the Serial Communication
option.
•Press and use or to highlight the
desired baud rate.
•Press to confirm your selection and return to the System
Setup menu or press to return to the System Setup menu
without changing.
Meter Information
This option provides general information about the instrument serial number (each instrument has a unique
identification serial number), the software version and the factory calibration date and time (for mV and
temperature).
:All instruments are factory calibrated for mV and temperature. One year after factory calibration, a
warning message “  ” will be displayed when powering up the instrument.
The instrument will still function, however, it should be taken to the nearest Hanna Customer Service for
factory calibration.
To view the Meter Information:
•Press while in
Measure mode.
•Press .
•Use or to select the Meter Information option.
•Press to acces the Meter Information menu.
•Press to return to the System Setup menu.
62
      
Press and then keys must be pressed on front
display to initiate this function. Real time continuous measurements
are on display with “” flashing and real time continuous
logging into meter memory, until the meter reaches the stability
criteria to go into Auto Hold mode. The stored sample logs will
be marked with an  to indicate the Auto Hold mode. The
virtual key returns operation to real time continuous measure-
ments and stops the logging session.
      
Press in order to add one new record in the log report. The manual log is working even if it is in
Auto Hold or Continuous reading mode. Press to initiate the Auto Hold event. “” will
flash until the stability criteria is reached and then the screen freezes in Auto Hold mode, the data is marked
with an .
       
Press and then keys initiate and automate the capture of stable data which is stored in the
Recall Manual Log file. During the process, “” will flash until the stability criteria is reached and
then the screen freezes in Auto Hold mode, the data is logged and marked with an . The virtual key
returns operation to Real time continuous measurement. Press again to log a second stable
data point. The lot ID along with the record index will appear for short time on the top/left corner on the
selected channel window, every time a record will be added to the lot.
LOG RECALL
This feature allows the user to view all stored data. If no data
was logged, the “   ” message will be
displayed on the LCD in the Log Recall screen. Otherwise, the
instrument will display all the memorized lots in accordance with
the selected option: Automatic Log, Manual Log or ISE Method
Report (  only).
To view the memorized data:
•Press while in Measure mode.
•Press . The “   ” message
will be displayed in the Reminder messages area.

20
Restore Factory Settings
This option allows the user to erase all user settings and reset the instrument to the default factory settings in pH
mode.
To restore the Factory Settings:
•Press while in
Measure mode.
•Press .
•Use or to select the Restore Factory Settings
option.
•Press to confirm your selection. A pop-up menu will be
displayed, asking for confirmation.
•Press to confirm your selection and return to the System
Setup or press to return to the System Setup menu
without restoring defaults.
•Press to return to Measure mode.
Software update
This function allows the user to update instrument software. In
order to start the PC upgrade application, you need to select the
proper baud rate, the software update package and start the
update.
61
LOGGING
  
)1(citamotuA goLcitamotuA
 )2(launaM goLlaunaM
)AN(dloHotuA elbacilppAtoN
)3(citamotuA goLcitamotuA
 )4(launaM goLlaunaM
)5(dloHotuA goLlaunaM
There are 5 ways the Reading Mode and Log may be configured together. The table below shows the
combinations and indicates where the completed log will be stored.
      
Real time continuous measurements are on display with continuous
logging to meter memory. Press .
      
Real time continuous measurements are on display and snapshots
of measurement data are stored in the Manual log when the user
presses . Subsequent snapshots will be added to the
same Manual Lot every time the is depressed unless
  is selected under Log options.
:When the is pressed the lot ID along with the
current record number will appear for short time on the
selected channel window on the top/left corner (e.g.
L033_MV 8 - this means lot ID L033_mV and recod
number 8).