Hardy Diagnostics QuickSlide HemaPRO Mounting instructions

Rev. 052316dc HemaPRO User Manual Page 2of 9
Table of Content
Introduction……………………………………………………………………………………… 3
1.0 Intended Use....................................................................................................4
2.0 Operator Responsibility - Safety Instructions.............................................4
2.1 Explanations of Safety Notes.................................................................4
2.2 Safety Instructions..................................................................................4
3.0 Instrument Setup............................................................................................5
3.1 Initial Start-Up .......................................................................................5
4.0 Operating Instructions...................................................................................6
4.1 General Guidelines.................................................................................6
4.2 Stain Procedure .....................................................................................6
4.3 Reagent Replacement.............................................................................6
4.4 Menu Options.........................................................................................7
4.5 Setting Fill Levels..................................................................................7
5.0 Maintenance....................................................................................................8
6.0 Service and Supplies.......................................................................................8
7.0 Safety Data Sheets ..........................................................................................9

Rev. 052316dc HemaPRO User Manual Page 3of 9
Congratulations!
You have made an excellent choice for your Lab. Hardy Diagnostics thanks you for the
trust you have placed in our products and services.
This operating manual has been designed to help you gain an understanding of the
operation and application of our HemaPRO. For optimal utilization of all functions, we
recommend that you thoroughly study this manual prior to beginning operation.
This manual has been prepared as an aid for all operations and maintenance, which
can be carried out in your facility.
The QuickSlide™Quality Management System
Our devices for the medical laboratory are developed, produced, and distributed
according to the requirements of ISO 9001:2008. All products are shipped to you from
our manufacturing facility located at 3411 McNiel Ave., Suite 302, Wichita Falls, Texas
76308.
Unpacking and Inspecting
Carefully unpack the HemaPRO and accessories. Check for damage incurred during
transit. Keep all packing material until you are sure the unit operates properly. Any
damage to the shipping box should be reported to the responsible carrier or postal
authority. These instructions must be followed for us to guarantee our full support of
your claim for protecting against loss from concealed damage.
Printed in U.S.A. Changes without prior notification reserved.
Important: Keep this operating manual for future use.
© 2016 All Rights Reserved
QuickSlide™™, a Division of Hardy Diagnostics
3411 McNiel, #302
Wichita Falls, TX 76308
800-295-9588
www.HardyDiagnostics.com
[e-mail]

Rev. 052316dc HemaPRO User Manual Page 4of 9
1.0 Intended Use
The HemaPRO hematology stainer is an automated, bench-top instrument designed for
the staining of hematology slides for in vitro diagnostic use. This instrument accepts
standard thickness 25mm x 75mm or 1” x 3” glass slides that are clean, specimen
applied, fixed and ready for staining.
Two settings are programmed—one for blood smears, the second for bone marrow—and
specific stain durations are customizable. A cuvette holds one slide at a time. The
chamber is flooded with stain, buffer, or rinse by use of peristaltic pumps under the front
cover. After staining and rinsing have been completed, the slide quality is best when
removed from the cuvette and dried. Do not leave the slide in the cuvette to air-dry.
2.0 Operator Responsibility - Safety Instructions
The HemaPRO ensures safe operation when installed, operated, and maintained
according to common safety regulations. This section explains all the potential dangers
that may arise when operating the HemaPRO and also specifies important safety
precautions to prevent mishaps.
The operator is responsible for the qualification of the personnel operating the
instruments. The operator is also advised to refer to this Operating Manual and/or call the
QuickSlide™ Technical Support phone number for additional assistance.
2.1 Explanations of Safety Notes
2.2 Safety Instructions
CAUTION:
Describes a possibly dangerous or harmful situation. If this is not
avoided, slight or minor injuries could result, product or anything in its
surroundings can be damaged.. A warning of possible property damage
may also be contained in the text.
CAUTION:
Instrument must to be placed on a level operating surface.
Read and understand all instructions and safety precautions listed
in this manual before installing and operating the unit.
Only use the required QuickSlide™ Wright Giemsa staining
reagents (Cat. no. QS-29000A).
Only use the required QuickSlide™ tubing kits (Cat. no. QS-
002).
Always make sure tubing is placed correctly and is not leaking.

Rev. 052316dc HemaPRO User Manual Page 5of 9
3.0 Instrument Setup
3.1 Initial Start-Up
3.1.1 Before plugging the wall transformer into an AC outlet, connect the round
barrel receptacle to the instrument. The Connector is located on the left-
hand side of the instrument directly under the on/off switch.
3.1.2 Make sure the power switch is turned off and then plug the wall
transformer into the unit.
3.1.3 Place the Reagent Pack on the shelf behind the instrument and connect the
Reagent Pack phone type connector to the receptacle on the right-hand
side of the instrument. If the Reagent Pack is not plugged in when the unit
is turned on, the message "SELF-TEST FAILURE: Pack not connected"
will appear. Turn unit off, connect Reagent Pack and turn unit on to
continue.
3.1.4 Remove the caps from the Reagent Pack and insert the color-coded tubing
cannulas in the appropriate bottles. Do not remove the heat seals on the
bottles. Push the tubing cannulas through the heat seals at an angle and be
careful not to damage the silicone tubing.
3.1.5 Place the RED waste tube in the waste container.
CAUTION:
The HemaPRO will only accept stain packs with an included module sold by
Hardy Diagnostics’ QuickSlide™ to ensure optimal results. The instrument is
calibrated only for these reagents. The use of other stain packs or solutions will
void the warranty.
Always turn off the unit and disconnect the power supply before
performing any service or maintenance procedures or moving the
unit.
Always remove the tubing kit cannulas from bottles before
moving the unit.
Transport the unit with care.
Dropping the unit may cause internal and external damage to the
instrument.
Never operate the unit with a damaged power supply.
Repairs are to be carried out only by qualified QuickSlide™
service personnel.

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4.0 Operating Instructions
4.1 General Guidelines
4.1.1 Make sure the specimen smears are thoroughly dry before placing them
into the cuvette.
4.1.2 Use high quality clean slides. We recommend ProSlide Premium, Frosted
End, Slides (Cat. no. PF72P). Slides that have variable thickness may have
variable staining results.
4.1.3 Replace the tubing every 6 to 12 months following the maintenance
protocol.
4.1.4 Do not leave the stained slide in the cuvette for an extended period of
time. It will not dry as rapidly and quality could diminish.
4.2 Stain Procedure
4.2.1 Turn the instrument on and the instrument will go through a self-test and
display prime or continue.
4.2.2 The unit needs to be primed at least two times before staining slides.
4.2.3 Press <F4>. Choose the desired type of stain and press <F1> for blood
smears or <F4> for bone marrow smears.
4.2.4 Press <F4> and the cuvette will be prepared.
4.2.5 Load the slide with specimen facing out to the front (towards you) and
press <STAIN>.
4.2.6 When done, remove the slide and press <F4> to continue.
4.2.7 Wipe the back side of the slide and let completely dry before viewing
under the microscope.
4.3 Reagent Replacement
4.3.1 Turn the instrument off.
4.3.2 Remove the cannulas from the empty Reagent Pack.
4.3.3 Unplug the phone connector from the instrument and remove the empty
Reagent Pack. Empty the waste container at this time.
4.3.4 Pace the new Reagent Pack on the instrument, connect the phone
connector and place the color-coded cannulas in the new appropriate
reagent bottles.
4.3.5 Turn the instrument on. The Self-Test menu will be displayed. The cuvette
is then automatically drained.
4.3.6 Prime the instrument twice before staining.
CAUTION:
When changing reagent kits, it is important to regularly check the waste bottle.
It must be routinely emptied to prevent overflow. If an overflow occurs, it can
damage the instrument and/or its surroundings.

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4.4 Menu Options
4.4.1 The MENU key allows you to customize your stain and buffer times for
both blood and marrow stains separately.
4.4.2 The stain and buffer times are changed in 5 second intervals. If times are
changed press <F4> to save your selected time. Factory default settings
are 30 seconds for stain and 30 seconds for buffer on the blood setting
and 60 seconds for stain and 60 seconds for buffer on the marrow setting.
4.4.3 This instrument allows users to set the Sound Beep to on or off.
4.5 Setting Fill Levels
The fill levels are factory set for a standard 1”x3” slide. However, the fill levels
can be customized to the operator’s preference.
4.5.1 Insert a blank 1”x3” slide into the cuvette. Slide must be loaded before
setting fill levels.
4.5.2 Unit must be primed before setting fill levels.
4.5.3 Turn unit off and then back on and wait for the main menu. Once the
main menu is displayed, turn the unit off again.
4.5.4 Turn the machine on while holding down <F4>.
4.5.5 Select <F4>.
4.5.6 If the cuvette has any fluid in it, press and hold <F1> until it is all gone.
4.5.7 If it is empty, then press <F4>.
4.5.8 To set stain level, press and hold <F2> until the desired level is reached.
Then release the key.
4.5.9 Drain the cuvette until it is completely empty and press <F4> again.
4.5.10 Use the <F4> key to set the rinse and buffer. They are set simultaneously.
Drain the cuvette again.
4.5.11 To save fill levels, press the MENU key. The unit will save the fill levels
and cycle back to the Main Menu to prepare for the next slide.
CAUTION:
Fill levels not set with a blank 1”x3” slide inserted in the slide chamber will be
incorrect and lead to overflow of reagents onto operating area. This can lead to
possible damage of the unit’s surroundings.

Rev. 052316dc HemaPRO User Manual Page 8of 9
5.0 Maintenance
The orange pump tubes and color coded lines must be replaced every 6 to 12 months
depending on use.
To perform this procedure, do the following:
5.1 Remove the lines from the stain bottles.
5.2 Prime the unit twice to remove fluid from the old tubes and expel it into the waste
container.
5.3 Shut the unit off.
5.4 Unscrew the two thumbscrews holding the pump cover in place.
5.5 Remove the lines one at a time and replace them, starting from left to right. The
longest lines are to be positioned on the left side of the orange pump tubes and the
shorter lines should be on the right side of the pump tubes. The longer lines are to
go into the reagent bottles and the shorter lines will go to the slide holder.
Pump #1 furthest to the left is the rinse (white labeled line).
Pump #2 is the buffer (green labeled line).
Pump #3 is the waste (red labeled line).
Pump #4 is the stain (blue labeled line)
5.6 After installation, prime the instrument two times to fill the buffer and rinse lines.
6.0 Service and Supplies
If a problem is encountered that is beyond the scope of this manual or additional
assistance is required, contact our QuickSlide™Technical Support team at
[phone].
If additional supplies are needed, reagents and tubing kits can be ordered through Hardy
Diagnostics’ Customer Service. Call [phone], or go to
www.HardyDiagnostics.com. Alternatively, you can contact your preferred distributor.

Rev. 052316dc HemaPRO User Manual Page 9of 9
Catalog Number
Description
QS-2900
HemaPRO Stain Kit
QS-002
HemaPRO Replacement Tubing Kit
PF72P
ProSlide, 1” x 3”, Premium Microscope Slide, 72/box
Pre-cleaned, Polished Edges, Frosted End
7.0 Safety Data Sheets
Safety Data Sheets (SDS) can be found at www.HardyDiagnostics.com/sds_search.html