PerkinElmer OMNI Prep User manual

OMNI Prep
Multi-Sample Homogenizer
User Manual
OMNI Prep
Multi-Sample Homogenizer

WARRANTY INFORMATION
This manual is a guide for the use of the OMNI Prep Automated Homogenizer and accessories.
Data herein has been veried and validated. It is believed adequate for the intended use of
the instrument. If the instrument or procedures are used for purposes over and above the
capabilities specied herein, conrmation of the validity and suitability should be obtained,
otherwise OMNI International, a PerkinElmer company does not guarantee results and
assumes no obligation or liability. This publication is not a license to operate under, or a
recommendation to infringe upon, any process patents.
Notes, cautions, and warnings within the text of this manual are used to emphasize important
and critical instructions.
This OMNI International, a PerkinElmer company product is warranted to be free from defects
in material and workmanship for a period of ONE YEAR from the date of delivery. OMNI
International, a PerkinElmer company will repair or replace and return free of charge any part
which is returned to its factory within said period, transportation prepaid by user, and which is
found upon inspection to have been defective in materials or workmanship. For the rst 90 days,
both parts and service are without charge. For the balance of the period, parts will be provided
but service will be charged at established labor rates. This warranty does not include normal
wear from use; it does not apply to any instrument or parts which have been altered by anyone
other than an employee OMNI International, a PerkinElmer company nor to any instrument
which has been damaged through accident, negligence, failure to follow operating instructions,
the use of electric currents or circuits other than those specied on the plate axed to the
instrument, misuse, or abuse. OMNI International, a PerkinElmer company reserves the right
to change, alter, modify, or improve any of its instruments without any obligation whatever to
make corresponding changes to any instrument previously sold or shipped.
THE FORGOING OBLIGATION IS IN LIEU OF ALL OBLIGATIONS AND LIABILITIES INCLUDING
NEGLIGENCE AND ALL WARRANTIES OF MERCHANTABILITY OR OTHERWISE, EXPRESSED OR IMPLIED
IN FACT OR BY LAW, AND STATE OUR ENTIRE AND EXCLUSIVE LIABILITY AND BUYERS EXCLUSIVE
REMEDY FOR ANY CLAIM OF DAMAGES IN CONNECTION WITH THE SALE OR FURNISHING OF
GOODS OR PARTS, THEIR DESIGN, SUITABILITY FOR USE, INSTALLATION, OR OPERATION. OMNI
INTERNATIONAL, A PERKINELMER COMPANY WILL IN NO EVENT BE LIABLE FOR ANY SPECIAL OR
CONSEQUENTIAL DAMAGES WHATSOEVER, AND THEIR LIABILITY UNDER NO CIRCUMSTANCES WILL
EXCEED THE CONTRACT PRICE FOR THE GOODS FOR WHICH LIABILITY IS CLAIMED.
- READ ALL INSTRUCTIONS BEFORE USING.
- SAVE THIS OWNER’S MANUAL.
The OMNI Prep has been engineered for maximum functionality as well as safety; however, basic
safety precautions and common sense must always be demonstrated when using any electrical
product. DO NOT attempt to modify any part of the OMNI Prep. If you experience problems with
or have questions about your OMNI Prep, contact your authorized dealer.
- DO NOT allow the machine to be submerged in any liquid.
- DO NOT use in any setting other than an indoor laboratory.
- DO NOT plug power cord into an incorrect outlet.
- DO NOT operate the product if it is damaged in any way.
- DO NOT use attachments not recommended by the manufacturer.
- Use this product only for its intended purpose as described in this booklet.

OVERVIEW
The OMNI Prep Homogenizer is a variable speed, multi-sample homogenizer. The OMNI Prep
is powered by a 150 watt, 30,000 rpm belt-driven motor. It includes a built-in high eciency
speed control, a digital speed display, and a built-in timer. The OMNI Prep operates exclusively
with Disposable Probes™ plastic generator probes or specially designed Stainless Steel Probes.
The motor drive unit housing has been specically designed to reduce motor noise to less than
75db at top speed. The OMNI Prep allows you to process up to 6 samples at a time in 1.5 - 50 mL
sample tubes.
Door
Lever Belt Drive
Window
Lift Plate
Thumb Tab Disposable Probes
Disposable Probes
Cradle
Sample Tube
(15 mL shown)
Floor Mat Linear
Actuator Control
Panel
Processing Rack
Toggle
Tube Holder
Dynamic
Tab Power Entry Module
Processing Rack

UNPACKING THE OMNI PREP
PLEASE NOTE: SAVE ALL INCLUDED PACKAGING!
1. Open the box on
the oor. Remove the
two rack boxes.
(Package may
contain one rack box
depending on your
order).
3. Lay the box down
on one side. Make
sure that the box
ap is folded under
(arrow).
5. Lift the box o of
the foam as shown.
7. Work the foam o
of the unit by lifting
from one side to the
other as shown. The
unit is held very tight-
ly in the foam.
2. Remove the
accessory box.
4. Turn the box
upside down making
sure that all of the
aps are folded out
as shown.
6. Lay the OMNI Prep
upside-down on the
oor.
8. Turn the unit over
and remove the rest
of the foam.

SPECIFICATIONS
Variable Motor Speed: 500 - 30,000 rpm
Speed Control Accuracy: +/- 1%
Dimensions: W: 15.7”(40 cm), D: 7.8” (20 cm), H: 11.8” (33 cm)
Weight: 21 lbs. (9.53 kg)
Electrical Requirements: 110-230 VAC 50/60 Hz
Standards Approval/Compliance: CE and UKCA certied
ACCESSORIES
The OMNI Prep is sold as a customizable homogenizing system. Listed below
are all available accessories for the OMNI Prep. At least 1 processing rack, 3
tube holders, and Disposable Probes are required to operate the OMNI Prep.
OMNI Prep Processing Rack
Movable for highly ecient process. Required for operating the
OMNI Prep.
OMNI Prep Rack Tube Holders
Tube holders are available for 1.5 - 2 mL, 15 mL and 30 or 50 mL.
sample tubes. Custom tube holder sizes are also available. Each
holder holds 2 sample tubes. Tube holders are required to operate
the OMNI Prep.
OMNI TipsTM Disposable Probes
25 OMNI TipsTM Soft Tissue - Order # 30750
50 OMNI TipsTM Soft Tissue - Order # 32750
100 OMNI TipsTM Soft Tissue - Order # 34750
500 OMNI TipsTM Soft Tissue - Order # 35750
25 OMNI TipsTM Hard Tissue - Order # 30750H
50 OMNI TipsTM Hard Tissue - Order # 32750H
100 OMNI TipsTM Hard Tissue - Order # 34750H
500 OMNI TipsTM Hard Tissue - Order # 35750H
2 mL
15 mL
30 or
50 mL

ACCESSORIES
Stainless Steel Homogenizer Probes
These Stainless Steel Probes were designed specically
for the OMNI Prep. These probes are ideal for larger
sample volumes, larger initial sample tissue sizes, and
tougher tissue types. Stainless Steel Probes are also
resistant to most organic solvents commonly used
during homogenization.
5 x 75 mm Stainless Steel Probe
Order # B5-075
(6 Pack) - Order # B5-075-6
7 x 110 mm Stainless Steel Probe
Order # B7-110ST
(6 Pack) - Order # B7-110ST-6
10 x 110 mm Stainless Steel Probe
Order # B10-110ST
(6 Pack) - Order # B10-110ST-6
7 mm Hybrid Probes
The Hybrid Probes combine the convenience of
disposable plastic probes with the durability of
traditional stainless steel probes. Hybrid probes are
made up of an outer stainless steel tube with an inner
Ultem plastic shaft. The plastic shafts can either be
disposed of after use or cleaned and reused multiple
times. The simple two-piece design makes these probes
much easier to clean and disassemble than traditional
stainless steel probes.
7 x 110 mm Hybrid Probe
Order # 22-007
(6 Pack) - Order # 22-007-6
OMNI Prep Ice Tray
Keeps frozen and sensitive samples cold during processing.
Ice Tray for 1.5mL—2mL Tubes - Order # 06-207
Ice Tray for 15mL—50mL Tubes - Order # 06-210

PREPARATION FOR USE
Connect the power cord to the power entry module on the rear panel, and
plug it into an appropriate outlet.
Note: The OMNI Prep is equipped with a universal power supply which works
with an input voltage of 110 - 230 VAC at 50/60 Hz.
WARNING: DO NOT attempt to plug a cord into an incompatible outlet.
WARNING: DO NOT attempt to remove the ground post on 115v cords. Ser-
vice power must be earth grounded.
CAUTION: It is strongly recommended that a surge protector be used
to help protect the OMNI Prep.
On/O Switch

LOADING SAMPLES
The processing rack has been engineered to hold up to six open or sealed
tubes of various sizes.
1. Determine the number of samples to be processed. Only an
even numbers of samples may be processed at a time.
- If processing only two samples, place the tube holder
between the two middle bars as shown.
(50 mL tube holder shown)
- If processing only four samples, mount the tube holders
on the rack leaving the rods on the far ends empty.
(50 mL holders shown)
- If six samples are desired, mount all three tube holders in
the rack as shown. (50 mL tube holders shown)
2. Load empty sample tubes into tube holders. Do not add
sample or uid to tubes until after a complete“dry”setup
has been made. (50 mL tube holders shown)
3. Install Disposable or Stainless Steel Probes by rst inserting
the lower end into the sample tube, then lower the Tip/
Probe until it rests securely in the probe cradle.
CAUTION: DO NOT allow probes to touch the bottoms of the tubes.
CAUTION: Only probes made by OMNI International, a PerkinElmer company are
compatible with the OMNI Prep. Attempting to use other style processors will
damage the OMNI Prep and void all warranties.

LOADING SAMPLES
PROCESSING RACK
The processing rack has been engineered to allow the
samples to be vertically oscillated relative to the probes during processing.
1. Load the tube holders, sample tubes, and Disposable/
Stainless Steel Probes by following the steps.
(50 mL tubes shown).
2. Loosen top adjustment knob on rear of rack.
3. Raise platform up to desired Tip/Probe depth in the sample
tubes. Lower top adjustment knob to platform and tighten.
4. Loosen lower adjustment knob on rear of rack.
5. Move platform down to desired Tip/Probe maximum
height in sample tubes. Raise lower adjustment knob to
meet platform and tighten.
CAUTION: DO NOT allow Disposable/Stainless Steel
Probes to touch the bottoms of the tubes.
CAUTION: DO NOT allow the Disposable/Stainless Steel
Probes to ever be completely extracted from the sample
tubes during processing.
6. Remove the Disposable/Stainless Steel Probes and the
sample tubes.
7. Load tubes containing sample and uid and replace the
Disposable/Stainless Steel Probes. The rack is now ready
to be loaded into the OMNI Prep.
NOTE: If stationary operation is desired, load the tube
holders as described and leave the processing
rack toggle switch in the O position during processing.
CAUTION: DO NOT use sealed tube caps when move-able
rack processing is desired. Rack will no move with tube caps
in place.
PLEASE NOTE: Portion of Disposable/Stainless Steel Probe
shown below the red line must be submerged in liquid
during processing.

LOADING THE ICE BATH
(OPTIONAL)
INSTALLING THE PROCESSING RACK
1. Install the empty ice bath to the rack underneath the tube holders.
2. Lower the tube holders so that the tubes are at the desired
depth relative to the ice bath.
3. Place the cooling agent (chilled water, ice, dry ice, etc.) into
the bath.
1. Ensure the lever is positioned all the way to the left; hold the
rack in your right hand using the thumb-tab located on the
center of the top plate.
2. Slide the rack onto the lift plate, and push completely back
until it stops.
3. Close the door and sweep the lever to the right until the
processing rack locks in its raised position.
CAUTION: The lever should move with smooth and constant
motion. If the lever does not slide smoothly as it is moved to
the right:
1. Slide the lever back to the left.
2. Remove the rack.
3. Rotate the tops of the probe shafts.
4. Replace the rack in the OMNI Prep.
Repeat this procedure until the locking lever can be moved
smoothly all the way to the right (fully locked position).

MODES OF OPERATION
The OMNI Prep is now ready for use.
Turn ON the power switch located on the rear of the unit.
The OMNI Prep has two modes of operation.
In Program mode, the active program denes the
process time and speed. Processing time counts down
and processing stops when time reaches zero.
In Manual Mode, the timer counts UP from zero, and the
OMNI Prep continues to process until the “Stop” button is
pressed (10 minute maximum).
Mode Line
Message Line
The OMNI Prep can store up to three separate programs. The active program
number is displayed on the mode line of the LCD screen (example: PROGRAM 1).
A program is modiable when it is active and the OMNI Prep is not running.
Modied time and speed values are automatically stored in the active program.
Press START to begin processing; the speed may be adjusted during a run, but
the time may not. The time will count down from the stored value. Processing
may be paused at any time by pressing the STOP button once or by lowering
the rack; the message line will read “RUN PAUSED”. Pressing START will resume
the run; the message line will read “RUN RESUMED” (rack must be locked).
Pressing the STOP button twice will cancel the run and reset the time to the
starting value.
PROGRAM MODE
Multi-sample Homogenizer
RUN
Speed Time
STOP
SPEED 30000 RPM
TIME 1:30 MIN
PROGRAM 1
OMNI Prep

The speed value is actively stored and may be changed at any time.
Press START to begin processing. Processing may be paused at any time by
pressing the STOP button once or by lowering the rack; the LCD display will
read “RUN PAUSED”. Pressing START will resume the run; the display will read
“RUN RESUMED” (rack must be locked). Pressing the STOP button twice will
cancel the run and reset the time to zero.
The processing rack is controlled by the toggle switch on the side of the OMNI
Prep. The mechanism raises and lowers the rack as the samples are processed
allowing more even/ecient processing. When in use, the rack oscillates
through the user specied range of motion, see section 3.2. The maximum
range of motion is about 2 inches. The rack oscillates at an approximate rate
of 0.8 Hz. The system is disengaged until the rack is raised into the running
position by sweeping and locking the lever to the right. Once the rack has
been installed, see section 3, and the Program to be run has been specied,
see sections 4.1 and 4.2, press the START button and turn on the movable rack
toggle switch.
MANUAL MODE
PROCESSING RACK OPERATION
MODES OF OPERATION

CLEANING THE OMNI Prep
CLEANING DISPOSABLE PROBES/TIPS
Remove power. Remove the rack and the oor mat and wipe with a mild
detergent and water solution. The OMNI Prep motor unit and tube holders
may be wiped down with a damp cloth of the same mild detergent. Do not use
an excess amount of cleaning solution. Dry thoroughly.
CAUTION: DO NOT attempt to submerge any part of the OMNI Prep in any
solution.
CAUTION: DO NOT spray cleaning solution on to the OMNI Prep. Wipe the
surfaces with a damp cloth.
CAUTION: DO NOT sterilize plastic tube holders in an autoclave. They may be
damaged if autoclaved.
While Disposable Probes can be autoclaved up to seven times for repeated
use, they are not recommended for extended use with phenol or guanidine-
based reagents. Disposal may be required when used in conjunction with
these reagents. However, for many applications the probe can be cleaned and/
or sterilized for reuse by the procedures described below:
Recommended Not Recommended
Sterilization
Techniques
• Autoclave up to 250°F
• Ethylene Oxide
• Formaldehyde
• Gluteralhyde (2%)
• Gamma irradiation up to 5MRAD
• UV Irradiation
Biological
Disinfection:
• Alcohol (70%)
• Hypochlorite (5%) [chlorine
bleach]
• Formaldehyde (40%)
• Phenolic Derivatives
• Extended exposure to
Guanidine
• Dichloromethane
Radioactive
Decontamination:
• Radiacwash
• Count-O
• Water/Ethanol/SDS

FUSE CHECK AND REPLACEMENT
1. Remove power. Unplug the cord from the power entry module.
The fuse holder is located in the center of the power entry module.
2. Use the tab on the right-hand side of the fuse holder and a screwdriver
(gure A) to slide the holder straight back until it is completely removed.
3. Check the fuse by examining the lament in the center of the tube as shown
(gure B). If the lament is broken, continue to step four. If the lament is in tact,
return the assembly into the power entry module and contact
OMNI International, a PerkinElmer company.
4. Un-clip the expired fuse and discard. Press on one end of the enclosed
portion of the fuse holder to remove the spare fuse from the casing as
shown (gure C). Replace the empty casing into the fuse holder.
5. Clip the new fuse into the exposed side of the holder and reinstall into the
power entry module.
6. Contact OMNI International, a PerkinElmer company to obtain a new
replacement fuse. Store the fuse in the casing on the enclosed side of the
fuse holder.
Figure A
Figure B
Figure C

CHECKING DRIVE BELT TENSION
The OMNI Prep operates by means of a belt driven motor system. Over time
the tension of the belt may need to be adjusted or the belt itself may need to
be replaced.
The OMNI Prep is equipped with a Belt Drive Window located on top of the
unit. This window allows for easy viewing of the Belt Drive Tensioner. The belt
tension is correct when the tensioner is located between the letters “B” and “C”
on the tension scale (See gure below).
If the belt tension is incorrect contact OMNI International, a PerkinElmer company.
CAUTION: ANY ATTEMPT TO REMOVE ANY SURFACE OR MODIFY THE OMNI
Prep IN ANY WAY WILL VOID ANY WARRANTY.
WARNING: To reduce the risk of electric shock, personal injury, or death,
DO NOT ATTEMPT TO REMOVE THE REAR PANEL OF THE OMNI Prep.
Tensioner

TROUBLESHOOTING
Do not attempt to service the OMNI Prep in a manner other than those
discussed in this manual. For any issue that is unsuccessfully corrected using
this guide, please contact OMNI International, a PerkinElmer company.
PROBLEM CORRECTIVE ACTION
The OMNI Prep is plugged in and
turned on but is not functioning.
-Check power cord connectors
-Check wall socket for power to the outlet
-Check Fuse
LCD screen is lit but motor will not run -Verify that the LCD displays“READY”
-Make sure the rack is locked
LCD lit but not functioning properly -Turn power o and back on to reset
Call if problem persists.
OMNI Prep begins a run but probes/tips
are not rotating
-Drive Belt may need servicing
-Turn the OMNI Prep OFF and call OMNI
International, a PerkinElmer company
Rack raises but will not lock -Do Not Force Lever.
-Verify that the rack is pushed completely
onto lift plate.
- Damaged drive connects on Disposable
Probes Tip shafts; replace the Disposable
Probes
Rack binds in transition to locked
position
-Do Not Force Lever
-Disposable Probe drive connects may
be damaged. Inspect and replace if
necessary.
-Verify that rack is securely pushed into
place.
Disposable Probes/tips stick in OMNI
Prep drives when unlocking the rack
-Disposable Probe drive connect worn,
replace Disposable Probe.
Excessive noise/vibration from Disposable
Probes
-Fluid level may be too low in tube.
-Drive Belt may need servicing
Excessive splashing in sample tube -Fluid level too low for tube size
-Contact OMNI International, a
PerkinElmer company about sealed tube
operation

NOTES

NOTES

935 Cobb Place Blvd. NW
Kennesaw, GA 30144
800.776.4431 • 770.421.0058
www.omni-inc.com PN: 03201 Rev. D