Harvard Bioscience BTX Oocyte Petri Electrode 45-2059 User manual

Oocyte
Petri Electrode
45-2059
45-2060
15 mm Oocyte Petri Electrode, Planum,
1 mm gap (electrode only)
15 mm Oocyte Petri Electrode, Planum,
1 mm gap, Kit (45-2059, 45-0503, 45-0204)
User’s Manual

2Publicaon 5502-018-REV 1.0
General Information
Warranty
BTX warranes this BTX Oocyte Petri Electrode for a period of 90 days from date of
purchase. At its opon, BTX will repair or replace the item if it is found to be defecve
as to workmanship or material. This warranty does not extend to damage resulng from
misuse, neglect, or abuse, normal wear and tear,
or accident. This warranty extends only to the original customer purchase.
IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR
CONSEQUENTIAL DAMAGES. Some states do not allow exclusion or limitaon of
incidental or consequenal damages so the above limitaon or exclusion may not apply
to you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS
FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do not allow this
limitaon on an implied warranty, so the above limitaon may not apply to you. If a
defect arises within the 90 day warranty period, promptly contact: BTX, 84 OCTOBER
 using our toll free number
 Goods will not be accepted
for return unless an RMA (Return Materials Authorizaon) number has been issued by
our customer service department. The customer is responsible for shipping charges.
Please allow a reasonable period of me for compleon of repairs or replacement and
return. If the unit is replaced, the replacement unit is covered only for the remainder
of the original warranty period dang from the purchase of the original device. This
warranty gives you specic rights, and you may also have other rights which vary from
state to state.
NOTE: BTX electrodes are not recommended for use with power supplies or cables from
other manufacturers. Such use is completely at the customer’s own risk as it may result
in damage, create unsafe condions and will immediately void the 90 day warranty.
IMPORTANT: Read all instrucons, warnings and precauons prior to use.
Technical & Customer Service
BTX® is the ulmate resource for technical informaon on the use of high voltage
transfecon and general electroporaon of molecules and drugs into ssue. We
constantly track and monitor scienc publicaons in this area. Our Technical Service
group extracts and enters pernent informaon, such as results and parameters from
these papers into a Protocol database. This database is available via the BTX website.
Please visit www.btxonline.com. For technical assistance, addional informaon or an
inquiry/request for repair service, contact BTX Technical Support/Customer Service
Group at:
BTX® A Division of Harvard Bioscience
84 October Hill Road

Toll Free: 1-800-272-2775 (U.S. only)


E-mail: [email protected]om
Internet: www.btxonline.com
If outside the United States and Canada: call 1-508-893-8999 or contact your nearest
BTX Distributor. A complete list of distributors is on our website.

3Publicaon 5502-018-REV 1.0
General Safety Information
Review the following safety precauons to avoid injury and prevent damage to this
product or any products connected to it. To avoid potenal hazard, use this product only
as specied. Only qualied BTX personnel should perform service procedures.
To Prevent Hazard or Injury:
ARCING CAN OCCUR AT HIGH VOLTAGES
An unfavorable combinaon of parameters such as high voltage sengs and a small
sample volume with a highly conducve medium might lead to ashover between the
electrodes (ARC) and/or explosive evaporaon of the medium. Reduce voltage or pulse
length to avoid repeang this condion.
DO NOT OPERATE WITH SUSPECTED FAILURES
If you suspect there is damage to the product, have it inspected by qualied BTX service
personnel.
DO NOT CONTACT ELECTRODES
To avoid re or shock hazard, observe all rangs and markings on the product or in this
manual before using the device.
AVOID EXPOSURE TO CONTACT
Do not insert ngers or try to remove electrode or sample during pulsing sequence.
WEAR PROPER EYE PROTECTION DURING ELECTROPORATION
DO NOT OPERATE IN AN EXPLOSIVE ENVIRONMENT
DO NOT OPERATE IN WET/DAMP CONDITIONS

4Publicaon 5502-018-REV 1.0
Introduction
The Oocyte Petri Electrode is a reusable petri dish type of electrode specically designed
for drug or gene delivery into oocytes and/or zygotes. The Oocyte Petri Electrode is
composed of two electrodes that are 3 mm wide by 15 mm long, polished medical grade
planum, arranged in parallel, with a 1 mm gap between them, and mounted on a glass
petri dish. This electrode can be used with a microscope so that individual oocytes can be
visualized during electroporaon. This electrode is parcularly useful for the generaon
of transgenic animals including use with CRISPR/Cas 9 constructs to both ‘knock-in’
and ‘knock-out’ targeted genes. The Oocyte Petri Electrode may be cleaned with a mild
detergent and sterilized with 75% ethanol. This electrode requires the use of Mini Micro-
Grabber Cables (45-0503) and Tweezertrode Adapter Cables (45-0204). This electrode is
compable with BTX ECM 830, Gemini X2, and ECM 2001+ Electroporaon units.
*Note: For compability with older models, call BTX Technical Support.
Important: Read all instrucons, warnings and precauons prior to use.
FOR RESEARCH USE ONLY

5Publicaon 5502-018-REV 1.0
1. Prior to use of electrode, spray with
Rain-X and gently shake over planum
electrode surface of electrode for
10 seconds. Discard Rain-X. Sterilize
Oocyte Petri Electrode by spraying
three mes with 75% ethanol, then
drying with a Kimwipe. Rinse with
sterile MilliQ water, then dry with a
Kimwipe. Electrode is now ready to use.
Note: Sigmacote may be used as an
alternave to Rain-X to coat electrodes
prior to use.
2. Connect the Mini Micro-Grabber cables
to the Oocyte Petri Electrode, plug the
banana cable into the Tweezertrode
Adapter Cables and then connect the
Adapter Cables into the voltage output
of the BTX Electroporator.
3. Following instrucons for the BTX
Electroporator, set the appropriate
parameters for electroporaon.
4. Use a wide bore pipee p to transfer
oocytes or zygotes to the 1 mm gap
between the two electrodes on the
slide. Suggested ll volumes are 7 µl
for 70 embryos and 10 µl for 100–120
embryos.
5. Deliver the electroporaon pulse(s) to
the sample.
6. Recover sample with wide bore
pipee p. During a session of
mulple electroporaons using the
same transfectant, excess buer may
be removed with a sterile eye spear in
between rounds of electroporaon.
7. Disconnect from Mini Micro-Grabber
cables and clean electrode by ushing
with dislled water.
8. Sterilize Oocyte Petri Electrode by
spraying with 75% ethanol three mes
and allow to dry.
9. Electrode is ready for next sample.
10. If nished with electroporaon,
remove Mini Micro-grabber cables
from Oocyte Petri Electrode.
11. Gently ush electrode gap with
a mild detergent and rinse with
dislled water.
12. Dry electrode with 75% ethanol by
ushing electrode with alcohol and
gently shake any remaining drops.
13. Allow to air dry unl no alcohol is seen
and store with glass cover in a dry
area.
Make sure the BTX Electroporator is switched o before connuing.
WARNING: High Voltage
Operation: Getting Started

6Publicaon 5502-018-REV 1.0
Specifications
Oocyte Petri Electrode Electrical & Technical Specicaons
Standard Capabilies:
Voltage Range 0 to 200 VDC (Do not use AC current)
Pulse Length Range 10 μsec to 10 sec
Pulse Number Range 1 to 99 (depending on voltage)
Operang Temperature 5º to 40ºC
Indoor use only
Relave Humidity 20 to 80%
Physical Characteriscs:
Item No. Electrode Length Electrode Gap Electrode Material
45-2059 15 mm 1 mm Medical Grade
Polished Planum
Compability:
Generators BTX Gemini X2, ECM 830, ECM 2001+
Please contact BTX Technical Service at any of the numbers
listed below in the event of any failure.
BTX®
A Division of Harvard Bioscience
84 October Hill Road

Toll Free: 1-800-272-2775 (US only)


E-mail: [email protected]om
Internet: www.btxonline.com
Troubleshooting