Thermo Scientific Applied Biosystems GeneChip 645 User manual

For Research Use Only. Not for use in diagnostic procedures.
GeneChip™ Hybridization Oven 645
USER GUIDE
Catalog Numbers 00-0331
Publication Number 08-0255
Revision E

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Products: GeneChip™ Hybridization Oven 645

GeneChip™ Hybridization Oven 645 User Guide 3
Contents
CHAPTER 1 The Hybridization Oven 645 . . . . . . . . . . . . . . . . . . . . . . . 4
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Quick reference guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Safety information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Unpacking and setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Unpacking the oven . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Operating the door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Using the rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Installing the rotisserie into the oven . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Operating the oven . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
The front panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Loading the rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Unloading the rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Communicating with the oven . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Serial communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Oven maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Replacing the fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
GeneChip™ Hybridization Oven 645 specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Physical dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Power requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Environmental requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Performance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Communications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Regulatory requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
China RoHS Restriction of Hazardous Substances compliance . . . . . . . . . . . . . . . . . . . . . . 26
China RoHS Restriction of Hazardous Substances compliance . . . . . . . . . . . . . . . . . . . . . . 27
Customer and technical support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Limited product warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

4GeneChip™ Hybridization Oven 645 User Guide
1The Hybridization Oven 645
Introduction
The Applied Biosystems GeneChip™ Hybridization Oven 645 (Figure 1) is a general
purpose laboratory oven with a rotating rotisserie designed for hybridization of
Applied Biosystems GeneChip™ cartridge arrays. Electronic controls accurately
govern the oven temperature and provide visible status information to the operator.
Key features of the oven include:
• Programmable temperature range from 30.0°C to 70.0°C
• Programmable rotation rate range from 10 rpm to 80 rpm
• Keypad programming
• Excellent temperature accuracy and uniformity
• Ergonomic design for ease of use
• Holds up to 64 GeneChip™ cartridge arrays
• Pause / Jog control
• Highly visible status indicators
• Status information available via RS232 serial port
• Stackable (2 units high)
• Error detection and shutdown in case of malfunction
The oven has a glass door for observing samples during processing. Interior surfaces
are of stainless steel for high durability and low maintenance.
Figure 1 The GeneChip™ Hybridization Oven 645.

GeneChip™ Hybridization Oven 645 User Guide 5
Chapter 1 The Hybridization Oven 645
Quick reference guide 1
Quick reference guide
The hybridization oven is very simple to operate (Table 1).
Table 1 Quick reference guide for the Hybridization Oven 645
If you want to: Then do this: Description
Turn on/off power. 1. Press the front, left I/O button.
2. Press PAUSE to view the current
settings and operate the oven.
Controls line power to the unit.
Adjust rotisserie motor
speed, pause the motor
or jog the motor.
1. Press the UP or DOWN button until
the set point blinks to access the
set point.
2. Press the UP or DOWN button to
set the speed (rpm).
3. Press PAUSE to pause the
rotisserie.
4. Press PAUSE again to take the unit
out of pause to program and run
the oven.
5. When the door is open, you can
use the PAUSE button as the JOG
button.
These controls adjust the speed of the rotisserie
motor.
Adjust the oven
temperature.
1. Press the UP or DOWN button until
the set point blinks to access the
set point.
2. Press the UP or DOWN button to
set the temperature (centigrade).
These controls are associated with the heating
function.

Chapter 1 The Hybridization Oven 645
Safety information
6GeneChip™ Hybridization Oven 645 User Guide
1
Safety information
These instructions contain important operating and safety information. You must
carefully read and understand these instructions before using the hybridization oven.
Applied Biosystems has designed the hybridization oven to optimize function,
reliability, safety, and ease of use. It is your responsibility to install and use the
hybridization oven properly.
Follow these safety instructions regarding the hybridization oven:
• This equipment is for indoor use only.
• Connect the unit to a properly grounded electrical outlet of the correct voltage
and current handling capacity. Check the nameplate on the back of the unit for the
voltage and current rating.
• Do not remove or modify the grounded power plug. Use only properly grounded
outlets to avoid a shock hazard.
• If replacing fuses, note that the oven employs double pole fusing. The power
entry module, located on the rear panel of the unit, contains two fuses, one for
each side of the AC power circuit. Replace these only with 5x20 mm F6.3A (fast
acting) 250V rated fuses, as marked on the rear panel of the oven. (See "Replacing
the fuses" on page 22.)
• Disconnect the unit from the power outlet before performing any cleaning,
maintenance, or service.
• The power cord is the disconnect device. Do not position the equipment so that it
is difficult to operate the disconnecting device.
• Always verify that the rotisserie has stopped turning before reaching into the
oven.
• The interior of the oven and its contents can reach temperatures that can cause
burns. Avoid immediate contact with the oven interior upon opening the door, or
wear protective gloves. The interior of the unit will remain hot without visual
indication for some time after the door is open or power is switched off.
• If you plan to use the unit with any procedure that will give off fumes, be sure to
operate the unit in a fume hood or with proper ventilation.
• The hybridization oven is not intended for use with flammable materials. If used
with flammable materials, fire or explosion hazard may result.
• If radioactive materials are used in processing, user must comply with all
regulations regarding the acquisition, use, and disposal of such materials.
• Do not continue to use the oven if the temperature or rotisserie controllers fail, as
evidenced by errors E01 through E12 being displayed (see the section, "Error
codes and troubleshooting" on page 21). Contact Thermo Fisher Scientific
Technical Support.
• Use appropriate hand and eye protection when handling hazardous chemicals.
CAUTION! If you use this instrument in a manner not specified by the
manufacturer, the protection provided by the equipment may be impaired and
the warranty may be voided.
WARNING! The oven weight is approximately 67 pounds (30 kg). To avoid
injury, use 2 persons when lifting the oven.

GeneChip™ Hybridization Oven 645 User Guide 7
Chapter 1 The Hybridization Oven 645
Unpacking and setup 1
• Replace the fuses with same type (see the section, "Replacing the fuses" on page
22).
NOTE: Verify that 115/230V switch is set correctly:
• 115 for Americas and Japan
• 230 for EU and most of the world
Standard IEC 320 power entry with fuse compartment on rear panel of oven
NEMA 5-15P (US standard) cord supplied with unit.
Local power cord must be supplied for EU, rest of world
t
Unpacking and setup
Each hybridization oven is shipped in a single carton. When unpacking the unit, check
each item against the packing list below. If something is missing, contact Thermo
Fisher Scientific Technical Support.
The GeneChip™ Hybridization Oven 645 accessories include:
• Rotisserie
• Eight plastic GeneChip™ cartridge carriers (Two are included in each box.)
– Red (Part No. 90-0356)
– Green (Part No. 90-0357)
– Purple (Part No. 90-0358)
– White (Part No. 90-0359)
• Eight spring steel carrier retaining clips (Part No. 10-0458)
• RS232 communications cable, 5 meters long, for serial port—workstation
communication
• Oven power cord (US NEMA 5-15P style standard)
CAUTION! DOUBLE POLE NEUTRAL FUSING. The power entry module
located on the rear of the unit contains two fuses, one located in each side of
the AC line.
IMPORTANT! In case of power interruption, if the interruption is no longer than
10 seconds, the oven will restart automatically at the originally programmed
temperature set point and rotation rate. If the interruption lasts longer than 10 seconds,
the oven will restart in PAUSE mode, in which both heating and rotation are
suppressed. You must press the PAUSE button to reactivate the oven. If you experience
frequent power interruptions in your locality, consider purchasing an uninterruptible
power supply (UPS).
WARNING! The oven is heavy. Two people are required to lift the oven.

Chapter 1 The Hybridization Oven 645
Unpacking and setup
8GeneChip™ Hybridization Oven 645 User Guide
1
Locating the oven • Use a strong, level table or laboratory bench capable of supporting the weight of
the oven. The oven weighs approximately 67 pounds (30 kg).
• Provide an adequate source of electrical power within reach of the power cord.
The oven operates on AC voltages from 100 to 240 volts nominal, 50/60 Hz, and
draws currents below 5 Amperes.
• Provide minimum clearance as follows: 0.5 inch (13 mm) from top, 2 inches (50
mm) from the back and each side, and 18 inches (460 mm) for door swing at the
front of the unit. Note that there is an air intake fan located in the rear panel. Do
not block this vent.
• Provide adequate bench clearance for cartridge preparation, loading and
unloading cartridge carriers.
• Avoid extreme ambient temperatures and locations close to heaters or air
conditioning vents.
• Keep the oven dry—avoid locations close to sinks or bulk liquid dispensers and
storage.
Unpacking the oven NOTE: The oven is packaged in a corrugated shipping crate that can be reuse if you
need to return the oven for service. However, if the crate has suffered significant
crushing, cutting or structural damage, and you need to return the oven to Thermo
Fisher Scientific, we recommend that you discard the crate and use new shipping
materials.
1. Remove the oven from its corrugated box and styrofoam protectors.
2. Place it on a sturdy table or laboratory bench.
3. Remove protective wrappings.
4. Pull on the handle to open the oven door and remove the box of accessories.
5. Verify that the oven chamber is free of paper, cardboard or other debris.
6. Push the door closed. It latches automatically.

GeneChip™ Hybridization Oven 645 User Guide 9
Chapter 1 The Hybridization Oven 645
Unpacking and setup 1
Rear panel items The following items are located on the rear panel (Table 2).
Operating the door 1. The door is hinged on the left of the unit and latches on the right. It opens with a
firm pull on its handle, located at the right side of the door.
2. When the door unlatches, if the oven is ON, the Door Open indicator will
illuminate, rotisserie rotation will stop and the heater will shut off.
3. The door swings open up to 180°, allowing easy access to the oven interior. Take
care not to forcefully overextend the door and ensure that the bench is clear of
objects that may be hit by the door.
4. Take care when opening the door while the oven is operating. The interior is hot.
Avoid immediate contact with the interior or wear protective gloves.
5. To close the door, swing it shut and press the door until it latches. Normal
operation will resume.
Table 2 Rear panel items.
Item What it does How to use
Power entry module /
line filter / fuse holder
Accepts power cord for providing line power to
unit. Filters incoming and outgoing power line
noise. Holds fuses for overcurrent protection.
Plug mating end of power cord into this
connector. Plug other end into suitable
power outlet.
Use your fingers or a screwdriver to
open the fuse holder to replace the two
fuses (See the section,"Replacing the
fuses" on page 22).
Voltage selector
You can manually change the oven voltage from
115V to 230V.
With a screwdriver, flip the switch to
select the required voltage.
Caution! Take care to set the correct
voltage for the oven. An incorrect
voltage setting will damage the oven.
Serial port
The serial port allows communication with the
workstation.
Connect the oven with the serial port of
the computer (RS-232).

Chapter 1 The Hybridization Oven 645
Using the rotisserie
10 GeneChip™ Hybridization Oven 645 User Guide
1
Using the rotisserie
Installing the
rotisserie into the
oven
Follow this procedure to install a rotisserie into the oven.
1. Set the power switch off and open the oven door.
2. Remove the rotisserie from the accessory box. Note that one end is equipped with
a rubber “foot” (Figure 2). The other end is equipped with a gear (Figure 5).
3. Place the rotisserie into the oven with the rubber mounted shaft end on the left
(Figure 4).
4. Insert the shaft into the holder. Line up the left side of the shaft with the pivot
point, then push the rotisserie to the left against the spring. Press the rotisserie
axle into the receptacle with sufficient force to displace the socket (Figure 3 and
Figure 4).
Figure 2 Detail of the left rotisserie axle, rubber foot.
Figure 3 Detail of the spring-loaded socket.