PerkinElmer Lambda Series User manual

HIGH-PERFORMANCE LAMBDA
SPECTROMETERS
Getting Started Guide
UV-VIS SPECTROSCOPY

Release History
Part Number Release Publication Date
L6050007 G November 2014
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer Ltd
Chalfont Road
Seer Green
Beaconsfield
BUCKS
HP9 2FX
United Kingdom
Or emailed to: [e-mail]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no
warranty of any kind with regard to this document, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in
connection with furnishing, performance or use of this material.
Copyright Information
This document contains proprietary information that is protected by copyright.
All rights are reserved. No part of this publication may be reproduced in any form whatsoever or
translated into any language without the prior, written permission of PerkinElmer, Inc.
Copyright © 2014 PerkinElmer, Inc.
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically marked as such,
are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.

Table of Contents
Introduction...............................................................................................5
About this Manual ............................................................................................. 6
Conventions used in this manual........................................................................ 7
Notes, cautions and warnings...................................................................... 7
Safety Practices .......................................................................................11
Overview.........................................................................................................12
Precautions......................................................................................................13
General Operating Conditions............................................................................14
Environmental Conditions .................................................................................15
Other Environmental Conditions .................................................................15
Electrical Safety ...............................................................................................17
Electrical Safety Issues ..............................................................................18
EMC Compliance ..............................................................................................19
Warning Labels................................................................................................20
Fuses ..............................................................................................................22
Moving the Instrument.....................................................................................24
System Description..................................................................................25
Overview.........................................................................................................26
Startup Procedure............................................................................................27
Further Information...................................................................................28
Installing your Lambda Spectrometer.....................................................29
Overview.........................................................................................................30
Site Requirements .....................................................................................30
Unpacking .......................................................................................................31
Connecting the System Components .................................................................33
Connecting to the PC.................................................................................33
Connecting to the Line Power Supply.................................................................34
Installing the UV WinLab Software ....................................................................35
Setting up the Instrument in the Software.........................................................44
Calibrating the Instrument .........................................................................49
Appendices...............................................................................................53
Appendix 1: WEEE Instructions for PerkinElmer Products....................................54
Appendix 2: Decontamination and Cleaning .......................................................55

Introduction

6 . High-Performance Lambda Spectrometers Getting Started Guide
About this Manual
This Getting Started Guide contains the safety information for your instrument and the basic
information you will need to get started with your instrument. It also includes the installation
details for a Lambda spectrometer (usually performed by a PerkinElmer Service Engineer).
For detailed information on your instrument, maintenance and troubleshooting procedures,
see the
High-Performance Lambda Spectrometers Hardware Guide (L6050008) on the
High-Performance Lambda Spectrometers User Manuals CD (L6050009).
Figure 1 High-performance Lambda Spectrometer

Introduction . 7
Conventions used in this manual
Normal text is used to provide information and instructions.
Bold text refers to text that is displayed on the screen.
UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. '+' is
used to show that you have to press two keys at the same time, for example, ALT+F.
All eight digit numbers are PerkinElmer part numbers unless stated otherwise.
Unless stated otherwise, High-performance Lambda spectrometer refers to the Lambda 650,
750, 850, 950, 1050 WB or 1050 NB spectrometer, as applicable.
Notes, cautions and warnings
Three terms, in the following standard formats, are also used to highlight special
circumstances and warnings.
NOTE: A note indicates additional, significant information that is provided with some
procedures.

8 . High-Performance Lambda Spectrometers Getting Started Guide
CAUTION
We use the term CAUTION to inform you about situations that could
result in serious damage to the instrument or other equipment. Details
about these circumstances are in a box like this one.
Caution (Achtung)
Bedeutet, daß die genannte Anleitung genau befolgt werden muß, um
einen Geräteschaden zu vermeiden.
Caution (Bemærk)
Dette betyder, at den nævnte vejledning skal overholdes nøje for at
undgå en beskadigelse af apparatet.
Caution (Advertencia)
Utilizamos el término CAUTION (ADVERTENCIA) para advertir sobre
situaciones que pueden provocar averías graves en este equipo o en
otros. En recuadros éste se proporciona información sobre este tipo de
circunstancias.
Caution (Attention)
Nous utilisons le terme CAUTION (ATTENTION) pour signaler les
situations susceptibles de provoquer de graves détériorations de
l'instrument ou d'autre matériel. Les détails sur ces circonstances
figurent dans un encadré semblable à celui-ci.
Caution (Attenzione)
Con il termine CAUTION (ATTENZIONE) vengono segnalate situazioni
che potrebbero arrecare gravi danni allo strumento o ad altra
apparecchiatura. Troverete informazioni su tali circostanze in un
riquadro come questo.
Caution (Opgelet)
Betekent dat de genoemde handleiding nauwkeurig moet worden
opgevolgd, om beschadiging van het instrument te voorkomen.
Caution (Atenção)
Significa que a instrução referida tem de ser respeitada para evitar a
danificação do aparelho.

Introduction . 9
WARNING
We use the term WARNING to inform you about situations that could
result in personal injury to yourself or other persons. Details about
these circumstances are in a box like this one.
Warning (Warnung)
Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu einer
Verletzung des Benutzers kommen kann.
Warning (Advarsel)
Betyder, at brugeren kan blive kvæstet, hvis anvisningen ikke
overholdes.
Warning (Peligro)
Utilizamos el término WARNING (PELIGRO) para informarle sobre
situaciones que pueden provocar daños personales a usted o a otras
personas. En los recuadros como éste se proporciona información
sobre este tipo de circunstancias.
Warning (Danger)
Nous utilisons la formule WARNING (DANGER) pour avertir des
situations pouvant occasionner des dommages corporels à l'utilisateur
ou à d'autres personnes. Les détails sur ces circonstances sont
données dans un encadré semblable à celui-ci.
Warning (Pericolo)
Con il termine WARNING (PERICOLO) vengono segnalate situazioni
che potrebbero provocare incidenti alle persone. Troverete
informazioni su tali circostanze in un riquadro come questo.
Warning (Waarschuwing)
Betekent dat, wanneer de genoemde aanwijzing niet in acht wordt
genomen, dit kan leiden tot verwondingen van de gebruiker.
Warning (Aviso)
Significa que a não observância da instrução referida poderá causar
um ferimento ao usuário.

10 . High-Performance Lambda Spectrometers Getting Started Guide

Safety Practices

12 . High-Performance Lambda Spectrometers Getting Started Guide
Overview
This chapter describes the general safety practices and precautions that must be observed
when operating your high-performance Lambda spectrometer.
This advice is intended to supplement, not supersede, the normal safety codes in the user's
country. It is also a supplement to the PerkinElmer standard Safety and Health Policy. The
information provided does not cover every safety procedure that should be practiced.
Ultimately, maintenance of a safe laboratory environment is the responsibility of the analyst
and the analyst's organization.
Please consult all manuals and CDs supplied with your high-performance Lambda
spectrometer and accessories before you start working with the instrument. Carefully read
the safety information in this chapter and in the other manuals supplied. When setting up the
instrument or performing analyses or maintenance procedures, strictly follow the instructions
provided.

Safety Practices . 13
Precautions
WARNING
Be sure that all instrument operators read and understand the
precautions listed below. It is advisable to post a copy of the
precautions near or on the instrument itself.
The following precautions must be observed when using your high-performance Lambda
spectrometer:
•Connect the instrument to a correctly installed line power outlet that has a protective
conductor (earth/ground).
•Do not attempt to make internal adjustments or replacements except as directed in this
handbook.
•Do not operate the instrument with any covers or parts removed.
•Servicing should only be carried out by a PerkinElmer Service Engineer or a similarly
authorized and trained person.
•Disconnect the instrument from all voltage sources before opening it for any
adjustment, replacement, maintenance, or repair. If, afterwards, the opened instrument
must be operated for further adjustment, maintenance or repair, this must only be done
by a skilled person who is aware of the hazard involved.
•Use fuses with the required current rating only and of the specified type for
replacement.
•Do not use makeshift fuses or short-circuit the fuse holders.
WARNING
If the equipment is used in a manner not specified herein the protection
provided by the equipment may be impaired.

14 . High-Performance Lambda Spectrometers Getting Started Guide
General Operating Conditions
Only use the high-performance Lambda spectrometer indoors and under the following
conditions:
Temperature 15 °C to 35 °C
Relative humidity 80% maximum (non-condensing)
If possible, avoid any adjustment, maintenance or repair of the opened, operating
instrument. If any adjustment, maintenance or repair of the opened instrument is necessary,
this must be done by a skilled person who is aware of the hazard involved.
Whenever it is likely that the high-performance Lambda spectrometer is unsafe, make it
inoperative. The high-performance Lambda spectrometer may be unsafe if it:
•shows visible damage;
•fails to perform the intended measurement;
•has been subjected to prolonged storage in unfavorable conditions;
•has been subjected to severe transport stresses.

Safety Practices . 15
Environmental Conditions
The instrument has been designed to be safe under the following conditions:
•Indoor use;
•Altitude up to 2000 m;
•Ambient temperatures of 5 °C to 40 °C;
•A maximum ambient relative humidity of 80% for temperatures up to 31 °C, decreasing
linearly to 50% relative humidity at 40 °C;
•Mains fluctuations not exceeding ±10% of the nominal voltage.
WARNING
This instrument is not designed for operation in an explosive
atmosphere.
Other Environmental Conditions
Chemicals
Use, store, and dispose of chemicals that you require for your analyses in accordance with
the manufacturer’s recommendations and local safety regulations.
WARNING
Hazardous Chemicals
Some chemicals used with this instrument may be hazardous or may
become hazardous after completion of an analysis.
The responsible body (for example, the Laboratory Manager) must
take the necessary precautions to ensure that the surrounding
workplace and instrument operators are not exposed to hazardous
levels of toxic substances (chemical or biological) as defined in the
applicable Material Safety Data Sheets (MSDS) or OSHA, ACGIH, or
COSHH documents.
Venting for fumes and disposal of waste must be in accordance with
all national, state and local health and safety regulations and laws.
OSHA: Occupational Safety and Health Administration (U.S.A.)
ACGIH: American Conference of Governmental Industrial Hygienists (U.S.A)
COSHH: Control of Substances Hazardous to Health (U.K.)

16 . High-Performance Lambda Spectrometers Getting Started Guide
Toxic Fumes
If you are working with volatile solvents or toxic substances, you must provide an efficient
laboratory ventilation system to remove vapors that may be produced when you are
performing analyses.
Waste Disposal
Waste containers may contain corrosive or organic solutions and small amounts of the
substances that were analyzed. If these materials are toxic, you may have to treat the
collected effluent as hazardous waste. Refer to your local safety regulations for proper
disposal procedures.
Deuterium lamps and other spectral lamps are maintained under reduced pressure. When
you dispose of lamps that are defective or otherwise unusable, handle them correctly to
minimize the implosion risk.
UV Radiation
Although the instrument is safe during normal use, you should be aware of the health
hazards presented by ultraviolet radiation. The deuterium lamp in the instrument emits
hazardous ultraviolet (UV) radiation. The radiation can cause serious damage to the eyes.
•When the deuterium (UV) lamp is illuminated, do not open the spectrophotometer
covers unless specifically instructed to do so in the manual.
•Always wear UV-absorbing eye protection when the deuterium lamp is exposed.
•Never look directly into the deuterium lamp.
Ozone
The deuterium (UV) lamp generates ozone while it is illuminated. Exposure to ozone can
result in severe irritation to the skin, eyes and upper respiratory system.
•ALWAYS ventilate the area surrounding the instrument such that the concentration of
ozone does not exceed the maximum permissible level (0.1 parts per million
(0.2 milligrams per cubic meter)). All venting must be to the outside air and never
within the building.
•Switch off the UV lamp when the spectrometer is not in use. You can switch off the
lamp using the Manual Control screen in UV WinLab by unchecking the lamp option and
clicking Apply.
Compressed Gases
Handle cylinders of compressed gas with care, in accordance with local regulations.
We recommend that gas cylinders be located outside the laboratory and the gases led to the
laboratory through approved gas supply lines.
Use only approved tubing, connectors, and regulators for gas supply lines.

Safety Practices . 17
Electrical Safety
The high-performance Lambda spectrometer has been designed and tested in accordance
with PerkinElmer specifications and in accordance with the safety requirements of the
International Electrotechnical Commission (IEC). The high-performance Lambda
spectrometer conforms to IEC 61010-1 (Safety Requirements for electrical equipment for
measurement, control and laboratory use) as it applies to IEC Class 1 (earthed) appliances
and therefore meets the requirements of the low-voltage EC directive 2006/95/EC.
This instrument is also certified by the Canadian Standards Association for safety of
laboratory equipment.
The instrument has been designed to protect the operator from potential electrical hazards.
This section describes some recommended electrical safety practices.
WARNING
Electrical Hazard
Any interruption of the protective conductor inside or outside the
instrument or disconnection of the protective conductor (earth/ground)
terminal is likely to make the instrument dangerous.
Intentional interruption is prohibited.
Lethal Voltages are Present In the instrument:
• Even with the power switch OFF, line power voltages can still be
present within the instrument.
• When the instrument is connected to line power, terminals may be
live, and opening covers or removing parts (except those to which
access can be gained without the use of a tool) is likely to expose
live parts.
• Capacitors inside the instrument may still be charged even if the
instrument has been disconnected from all voltage sources.

18 . High-Performance Lambda Spectrometers Getting Started Guide
Electrical Safety Issues
To ensure satisfactory and safe operation of the instrument, it is essential that the
green/yellow lead of the line power cord is connected to true electrical earth (ground).
If any part of the instrument is not installed by a PerkinElmer Service Engineer, make sure
that the line power plug is wired correctly:
Terminal Cord Lead Colors
International USA
Live Brown Black
Neutral Blue White
Protective Conductor (earth/ground) Green/Yellow Green
NOTE: The instrument must always be positioned so that it can be easily disconnected from
the power supply.

Safety Practices . 19
EMC Compliance
EMC directive
This product has been designed and tested to meet the requirements of the EMC directive
2004/108/EC.
FCC rules and regulations
This product is classified as a digital device used exclusively as industrial, commercial, or
medical test equipment. It is exempt from the technical standards specified in Part 15 of the
FCC Rules and Regulations based on Section 15.103(c).

20 . High-Performance Lambda Spectrometers Getting Started Guide
Warning Labels
WARNING
When this label is attached to an instrument it means refer to the
manual.
The following electrical warnings are shown on the rear of the instrument, as identified in
Figure 2:
Figure 2 Warning labels on the high-performance Lambda spectrometer
Warning symbols shown on
the spectrometer housing