Heraeus MEGAFUGE 1.0 User manual

MEGAFUGE 1.0
MEGAFUGE 2.0
MEGAFUGE 1.0 R
MEGAFUGE
2.0 R
MEGAFUGE
3.0 R
Megafuge 1.0
r.eraeus
Operating Instructions
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QUEST 1054

PRECAUTIONS AND HAZARDS
Please read this manual very carefully before attemptingto operate the centrifuge!
The centrifuge must not be operated by unqualified personnel1 Avoid causing damage to the un it or its
accessories through incorrect operation!
In the event oferrors, proceed exactly according to the instructions1
Nevertransportthe centrifuge with a rotor installed on its shaft!
Use only original spare parts!
ATTENTION -DANGER!
Never open the lid manually while the rotor is spinning!
It is forbidden to program a rotor or buckettype, which is not physically installed (see 7.2.1 ).
The following rules must be strictly observed:
-Neveroperate the centrifuge if the rotor is not correctly installed (see 5.1).
-Do not operate the centrifuge with any parts or covers removed.
-Never run the centrifuge when the electrical or mechanical equipment have been tampered wi th by
unauthorized or unskilled personnel.
-Never attempt to run the centrifuge with an incompatible rotor (see 2.4 - It is forbidden to use the rotor
#8155with any buckets in the Megafuge 1.0 or 1.0R).
-Do not operate the centrifuge with incorrectly installed buckets.
-The maximum rotor load and speed must be observed.
-Do not spin corrosive samples which may impair the material strength of the rotors and buckets without
taking all necessary precautions (e.g. sealed rotors or buckets).
-Never use accessories (rotors or buckets) which show noticeable traces of corrosion or mechanical
damage.
-Do not spin samples which can create flammable or explosive mixtures when exposed to air, unless
adequate safety precautions have been taken (e.g. shel ter).
-Do not operate the centrifuge in explosion-hazardous locations. The Megafuges are neither explosion-
proof norinert gas shielded.
-Do not use accessories which are not approved by Heraeus, except commercially available centrifuge
tubes of glass or plastic.
The manufacturer is only responsible for the security, reliability and performance of the unit, if
- the unit is operated in accordance with the operating instructions
-installations, expansions, new adjustments, changes or repairs are performed by Heraeus authorized
personel
NOTE:
When performing centrifugation and other functions which may expose workers to splashed blood or body
fluids, all laboratory personel must follow universal laboratory precautions including wearing gloves, facial
protection, gowns or laboratory coats, and plastic aprons. For further details, consult your local laboratory
safety director.

FAST START UP REFERENCE GUIDE
Setting up the MEGAFUGE
1. Set unit on a flat sturdy, resonance-free table (or lorrywith lockable wheel s) in a well vent il ated area (without
exposion to direct su nl igh t) and mark a safety zone of 30 cm around it (details in Section 4. 1 ).
2. Insure tho ma ins supplyavailable isequal with t t1e required on identification plate (see Electrical Connec tion,
Section 4.2).
3. After plugging into the adequatewall socket, turn on the main power loca.ted on the rear panel (left hand side).
4. Wail for the illumination of the yellow L ED n ex t to the LID key. Open the lid by pressing the LID keypad and
remove all pack i ng materials and accessories.
5. Remove the rotor packaging and mount it on the dr i ve shaft (see Secti on 5.1 + 5.2).
6. Rot or must move freely, when installing the swing-out rotor a ll four buckets must be occupied!
7. Load a ll adap tors and lubes mak ing sure the rotor is symmetrically balanced (see Symmetrical Charge,
Section 6.1).
WARNING!
Do not centrifugate dangerous material without taking all necessary precautions (see PRECAUTIONS
AND HAZARDS)I
8. Close the l id firmly, bul do not slam it. The "Open" message will disappear from the displ ay, if th e lid is
closed correctly.
Programming the First Run
9. To set the Cat. No. of lhe installed rotor press the SET key of the TI ME field once and then pressagain and
keep pressed for the whole se tting procedure. The rotor sign "r o" will appear and the flash ing Cat. No.
shown in th e SPEED/RCF field can now be changed by using the yellow "+" or "-" keys. If the flashing
Cat. No.is eq ualwith the insta ll ed one,release the pressedSET key (seeProgramming ofRotor,Section 7.2.1 ).
WARNING!
On using the swing-out rotor, always program the Cat. No. of the really installed buckets, otherwise the
maximum allowed bucket speed can be overwritten and damageto persons or propertymay result.
10 .To se t time, press the SET keypad of the TIME fi el d. Cha n ge time by press in g the"+" or "- " keyp ads (see
Programming of Tim e, Section 7.2.5).
11. To set speed for a timed run, press the SET keypad in t he SPEED field. Change speed setting by pressing
"+" or " -" keypad (for setting RCF i ns t ea d of SPEED values see 7.2.2).
12 . For timed runs, press the START keypad. Slow and fast acce leration can be togg led with the BRAKE key
(see Smoo th Acc eleration and Deceleration, Secti on 7.1.5). After termination of the programmed time, unit
will shut off automatically.
Preparing the next run
13. Wait for release of LID key (yellow LED indicates end of cycl e), open the lid and remove all tub es from
the rotor.
14. Ch eck for broken glass or leakage into roto r, bucketsand /o r chambe r (see 10.1 and for disinfecting see10.2).
ATTENTION!
In case of contamination, the user is obligated to disinfect the unit and its accessories.
15. To remove roto r, unscrew and grasp it on op po s in g points and detach from the shaft by pul li ng vertically
to the top.
Caution!
Remove the rotor only in vertical position otherwise the drive shaft will be damaged .
To pre pa re the next run, foll ow the routine cleaning procedures (of Section 10), if necessary,then repeal steps
5 to 15. For more detailed informa ti on abo ut the M EG AFUGE feature s, operati on and gu idelines, consult other
sections of th is manual.
3

TABLE OF CONTENTS
Page
PRECAUTIONS AND HAZARDS 2
FAST START UP REFERENCE GUIDE 3
1.
DESCRIPTION 6
1.1
Classification
6
1.2 Definition
6
1.3
Safety Inspections, Standards and Regulations
6
1.4
Standard Accessories
6
1.5
Warranty
7
2. TECHNICALDATA 8
2.1
Performance Features 8
2.1 .1 Com mon Features
8
2.1.2
Special Features of the standard MEGAFUGES 8
2.1 .3
Special Features of the refrigerated MEGAFUGES
9
2.2 "M EGACONTROL"-Features 9
2.3 Mains Supply
10
2.4 Rotor Tables 10
2.4 .1 MEGAFUGE 1.0 Rotors 10
2.4.2 MEGAFUGE 2.0 Rotors 10
2.4.3 MEGAFUGE 1.0R Rotors
11
2.4.4 MEGAFUGE 2.0 R Rotors
11
2.4.5 MEGAFUGE 3.0 R Rotors
11
3. SAFETYSYSTEMS
12
3.1 Rotor Chamber
12
3.2 Lid Lock
12
3.3
Thermal Motor Protection
12
3.4 Imbalance Switch
12
3.5
Check of Acceleration Rate (only MEGAFUGE 1.0R/2.0R) 12
3.6 Emergency Lid Release
13
3.7 Overtemperature Protection (only MEGAFUGE 1.0R/2.0R/3.0R) 13
4. INSTALLATION 14
4.1
Location
14
4.2
Electrical Requirements
14
4.3 Main Power Switch
14
4.4 Opening the lid
14
5. ROTOR EXCHANGE 15
5.1 Use of Collet Chuck 15
5.2 Mounting 16
5.3 Removing 16
6. ROTOR LOADING 17
6.1 Swing-out Rotors 17
6.2 Symmetrical Charge 17
6.3 Distribution of Racks 18
6.4 Tube Filling
18
6.5 Partial Load
18
6.6 Loading limits
19
6.7
Sealed Rotors # 3360/ # 5315
19
4

TABLE OF CONTENTS
7. "MEGACONTROL" PROGRAMMING INSTRUCTIONS
7.1 Co ntrol Keys
7.1.1 Lid
7.1.2 Start
7.1.3 Stop
7.1.4 Quick Run
7.1.5 Smooth Acceleration and Deceleration (brake)
7.2 Programming
7.2.1 RotorSelection
7.2.2 Speed Selection
7.2.3 RCF Selection
7.2.4 Brake Cut-out Speed Selection
7.2.5 Time Selection
7.2.6 Temperature Selection (o nly MEGAF UGE 1.0R/2.0R/3 .0R )
7.3 D is pl ay
7.3.1 Speed
7.3.2 RCF
7.3.3 Time
7.3.4 Rotor
7.3.5 Standby
7.3.6 Temperature (only MEG AF UGE 1.0R /2.0R/3.0 R)
8. ACCELERATION AND BRAKING PROFILES
8 .1 Profile Principles
8.2 Sel ec tion of Profiles
8.3 Turbule nce During Deceleration (Swing-out Rotors)
8 .4 Brake Setting for DelicateSamples
9. TEMPERATUREFEATURES
9.1 MEGAFUGE 1.0/2 .0 with Air Circulation Cooli ng
9.1.1 Pr epa ring a Speed-Temperature Ch art
9.1.2 Thermo-Deli cate Sa mples
9.2 MEGAFUGE 1. 0R/2.0R/3.0R Cooling Unit -Temperature Control
9.2.1 Pre-cooling or pr e- h ea ting of Empty Rotors and Buckets
9.2.2 Pre-cooling or pre -heating of Samp l es
10. MAINTENANCE AND CARE
10 .1 Cleaning
10 .2 Disinfection
10 .3 Collet Chuck
10.4 Grea sing
10 .5 Glass Breakage
10 .6 Condensed Water
10.7 Servicing Schedule
11. TROUBLE SHOOTING
11.1 Measures to Do-it-yourself
11.2 Servicing
Page
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23
24
24
24
24
25
25
25
26
26
26
26
26
27
27
27
28
28
30
5

1. DESCRIPTION
1.1 Classification
Tt1e MEGAFUGES are technically hi gh ly developed microprocessor controlled laboratory cen tr ifuges with
frequency controlled and maintenance-free induction rnolor.
The MEGAFUGES are bench-top mode ls with an ext ensivG range of accessories.
1.2 Definition
The centrifuges have low vibra ti on solid polymer bo tt om plat es. The housing is built of sheel steel. Tt1e front
panel is made of plastic. Housing and front panel are fixed tightly to th e bottorn plate.
The rotors are staned directly with a frequency controlled induction d ri ve (brushlcss) with high acceler a1 ion
power. Ihis drive ensures quiet, low vibration runr1ing at high speed wi th hi gh reliabili ty. The drive is mounted
with shock absorbers to the bottom plate and sealed wi th a rubber flange to the rot or chamber. The main
microprocess or controls the functions fo r speed measuring and regulation, the temperature measuring and
regulation (MEGAFUGE 1.0R/2. 0R/3 .0R) program storage, safety control and error coding. An addilional
microprocessor is responsible fo r the key and di sp l ay !unc ti ons. The centrifuge is controlled by the
"Megacontrol" system (see section 7.)
MEGAFUGE 1.0/2.0
The air, drawn through the rear hole of the lid during ope ration of the motor, cools the rotor, motor and
electroni cs and is discharged fro m the centrifuge via louvers in th e housing (see fig .1 ).
MEGAFUGE 1.0R/2.0R/3.0R
These mode ls are equipped with a compressor plant ro r temperature control (see section 9. 2).
1.3 Safety Inspections, Standards and Regulations
Safety inspections accordi ng to GS, CSA, CLA, U L, VOE and SEV.
- Acc ident prevention regulations for ce n1 rifuges, UVV VBG 7z.
- Acc ident prevention regulations fo r e le c tr ical plants and supplies U VV VBG 4.
-Electrical safety according to IEC 1010-1and I EC 1010-2-D
- Electrical noise suppression according to VOE 0871 grade 8
-DIN 58 970, part 2 for laboratory centrifuges
-MEGAFUGE 1.0R/2.0R/3.0R:Acciden1 preventation regulations for coo ling units, UVV VBG 20.
1.4 Standard Accessories
The centrifu ges are supplied with a collet chuck and a special socket wrench to fasten the rotors. (Spare
Pan N o's see below).
Centrifuge
MEGAFUGE 1.0/2.0R/1.0R/2.0R
MEGAFUGE 3.0R
Collet ct1uck
/ /70003100
,,?70904041
Socket wrench
//2036 0047
// 20005492
50 ml of anti-corrosive o il (Spare Part No. #7 00 098 24) to pro tect the metall ic surface of rotors and buckets
and a small vial of special grease (Spare Part No. #70006692) to coat the trun ni ons or sw in g out rotors are
su pp lied as well.
Printed documentation supplied are:
- a prospectus with all avail ab le accessories
- the operating instruc ti ons
- a notice card fort he correct fas te ning ofrotors
- a warranty registration form
- a pl as tic case for keeping all documentation (should t)e fastened aside the centrifuge)
6

1.5 Warranty
The vvarranty pe ri od starts wi th me day of delivery. Within lhe wa rranty pe r iod lhe cen tr ifuge is repaired or
re placed t ree of cost if tt1ere are clernonstrable faults in ma terials or workmanship.
0
(!)
0
Fig. 1 MEGAFUGE 1.012.0- lidshowing the holes for integrated air circulation
7

2. TECHNICAL DATA
2.1 Performance Features
2.1.1 Common Features
Base construction:
Housing:
Front panel:
Operation:
Drive:
Lid opening:
Closing:
Locking at run:
Starting/Stopping:
Re-starting:
Ouick-run/-stop:
Imbalancestop:
Range ofambient temp.:
-during operation
- in case ofstorage
or shipping
solid polymer bottom plate ensures l ow vibration centrifugal runnings
motorand back panel made of sheet steel covered wi th annealing color
synthetic resin for incorporation of key and displayelementscovered with cleanable
membrane.
By programming ofMEGACONTROL (features see 2.2)
frequency controlled, maintenance-free induction motor
key button, !he lid pops up and opens by the gas lid stay(s)
press the lid down and push on top of the lid locks.
doub le safety circuit (hard- and software controlled)
dualfunction key button indicated by LED's (start at green, stop at red)
at anytime during braking phase
wi th quick-run press-and-hold key/quick stop on release
automatic dur ing acceleration, if allowed imbalance is exceeded
4°C - 35 °C (39° F- 95 ° F)
-10°C - 50°C (14°F - 122°F)
2.1 .2 Specia l features of the standard MEGAFUGES
MEGAFUGE 1.0 MEGAFUGE 2.0
max. speed: 6000 rpm
6000 rpm
w it h rotor: 1/3 360
//33 60
max. RCF: 6240 X g
6240 X g
with rotor: /1 3360
1/ 3360
max. power in put:
700W
700W
max.capacity: 4 x 220 ml 4 x 500
ml
with rotor/bucket: #2704/#2706
# 81 55/# 8160
max. kin. energy:
26 .0 kNm 29 .8 kNm
max. sample overtemp.: 15 K 15 K
(above ambient temp.)
rotor chamber -material: ename lled steel ename
ll ed steel
- si ze(¢ x H): (430x210) mm
(430x210)mm
(1 7" X 8¼")
(17" X8¼")
dimension (HxWxD):
(41 x46x54)cm
(41 x46x54)cm
(16¼" X 18 ¼" 21¼") (16¼" X
18¼" X 211/4'')
weight:
52 kg
55 kg
115 pounds 1
22 pounds
noise level: ~ 70 dB(A)
~ 70 dB(A)
8 I

2.1 .3 Special features of the refrigerated MEGAFUGES
-MEGAFUGE 1
.OR MEGAFUGE 2.0 R MEGAFUGE 3.0 R
max. speed: 15000 rpm 15000 rpm 4 700 rpm
with rotor: # 1379/ #30 41 # 1379/ //304 1 /1 5315
max. RCF: 20120 X Q 20120 X g 4 790 xg
with rotor: II 1379 /11379 11 5315
max. power input: 1100W 1100 W 1450W
max. capacity: 4 X 220 ml 4 X 500 ml 4 x 750
ml
with rotor/bucke t:
,¥ 2704/#2706 #8 155/ #8160 118074/11 8080
max. kin. energy: 26.0 kNm 29.8 kNm 40.6 kNm
rotor chamber -material: fine steel fine steel fine steel
- size(¢ x H): (430x210)
mm (430x210) mm (460x230) mm
(17" X 8¼") (17" X 8¼") (18" X 9")
dimension (H xWxD):
(41 x71 x58)cm
(41 x71 x58)cm (44x 73 x60) cm
(16¼" X 28" 23") (16¼" X 28"X 23") (171/2'' X 29" X 24'')
weight: 104 kg 109kg
128 kg
230 pounds 240 pounds 282 pounds
noise level: ~ 64 dB(A)
~64 dB(A) ~ 64 dB(A)
2.2 "MEGACONTROL"-Features
Parameter storage:
Data memory:
Diagnostic indications:
Digital parameter display:
Acceleration:
Deceleration:
Speed selection:
RCF se l ect ion:
Brake cut -out point:
Rotor selection:
Time selection in 2 modes:
"hd" mode:
min mode:
Time display by Quick-Run:
sec mode:
MEGAFUGE 1.0R/2.0R/3.0R
Temperature selection:
for speed, RCF value, rotor type, brake cut-out point, running time
MEGAFUGE 1.0R/2.0R/3.0R: alsotemperature
with NV -R AM's (non -volatile random -access-memory)
-lid open
-imbalanced loading
-general troubles in the unit
-data transmitting errors
-rotor over temperature (MEGAFUGE 1.0R/2.0R/3.0R)
speed, RCF, rotor, time MEGAFUGE 1.0R/2.0R/3.0R: alsotemperature
2 stepped reproducable profiles: smooth start and fast, can be switched over
at any time using the brake key
1reproducable profile and unbraked deceleration, can be switched over at
anytime using the brake key
adjustable in stages of 100 rpm up to max. rotor or bucket speed
adjustable to max. ref value (see 2.4) converted into stages of 100 rpm
adjustable in stagesof 1 00 rpm up to the preselected speed
preprogrammed menu, arranged in ascending rotor or bucketCat. Numbers
continuous operation
adJustable in minutes from 1 min to 99 min
1 s - 99 s
0°c - 40°c
9

2.3 Mains Supply
Centrifuge Cat. No.
Voltage/ Frequency
MEGAFUGE 1.0
// 3490
230V /50Hz
--
MEGAFUGE 1.0
#349 1
120V/60Hz
MEGAFUGE 1.0 //3 495
100V 50/60Hz
MEGAFUGE 1.0 // 3496
240V 50 Hz
MEGAFUGE 2.0
#3482
230V /50Hz
MEGAFUGE 2.0 //3 485
120V /60 Hz
MEGAFUGE 1.0R
//3060
230V /50Hz
MEGAFUGE 1.0R
#3 06 1
120V /6 0 Hz
MEGAFUGE 1.0R #
3066
1 00V /50Hz
MEGAFUGE 1.0R //306 7
100V /60Hz
MEGAFUGE 2.0R
#30 80
230V /50Hz
MEGAFUGE 2.0R
// 3085
120V /60Hz
MEGAFUGE 3.0R
#8 100
230V/50Hz
MEGAFUGE 3.0R
#8 107
208/240
1) V
/60 Hz
ATTTENTION!
') Voltage for use: see identification label.
2.4 Rotor-Tables
2.4.1 MEGAFUGE 1.0 Rotors
Rotors with Swing-out Buckets
rotor/bucket max.
Cat.-No. capacity
[# ] [ml]
2705 + 2252
48 X 15
2705 + 2706
1) 4 X
220
2705 +27083) 8 plates
33604) 48 X 15
2704 + 8030
5)
48 X 15
2.4.2 MEGAFUGE 2.0 Rotors
Rotors with Swing-out Buckets
rotor/bucket max.
Cat.-No. capacity
[#]
[ml]
33604)
48 X 15
8155 +8082 2 carriers
8155 + 8160
4 X 500
1) round buckel with her
me tically tight lid
•) vacutain cr (tubes up to 1 32 mm length)
3) 4 microtiter carriers w,th 2 plates in each
max.
speed
[rpm]
4000
4300
2800
6000
4000
max.
speed
[rpm]
6000
3300
3500
ATTENTION -DANGER! The maximum speed of 2800 rpm must never be exceeded!
4) sealed rotor -ATTENTION! Instructions
in Section 6.7 must be observed absolutely!
5) rectangle bucket with her
me tically sealedl id
max.
RCF
[xg]
2890
3490
1280
6240
3220
max.
RCF
(xg]
6240
1850
2570
-
Power input Main Fusing
700W
16A
700W 15A
700W 15A
700W 16A
700W
16A
700W
15A
1100W
16A
1100W 15A
1100W 15A
-
1100W
15A
1100W
16A
-
1100W
15 A
1450W
16A
1450W
15A
max. max. load
radius
per position
[cm] [g]
16.2 510
16.9
425
14.6
360
15.5
510
18.1
510
max. max. load
radius
per position
[cm]
(g]
15.5 510
15.2 260
18.8
800
ATTENTION-DANGER! Hermeticalsealing is only guaranteed by troublefree operation of the centrifuge and its accessories!
10

2.4.3 MEGAFUGE 1.0R Rotors
Rotors with Swing-out Buckets and Angle Rotors
rotor/bucket max. max .
Cat.-N o.
capacity speed
[// ] [ml] [rpm]
1379
24 X 1 .5 15000
2705 + 2252
48 X 15 4000
2705 + 2706
1) 4 X 220
4300
2705 + 2708
3) 8 plates 2800
3041
18 X 2.0 15000
33604)
48 X 15 6000
2705 + 80305) 48 X 15
4000
2.4.4 MEGAFUGE 2.0R Rotors
Rotors with Swing-out Buckets and Angle Rotors
--- ---
rotor/bucket max.
Cat.-No. capacity
[ tf ] [ml]
1379
24 X 1.5
3041
18 X 2.0
3360
4) 48 X
15
8155 + 8082
2 carriers
8155+8160
4 X 500
1) rou
nd bucket with hermetically tight l id
2) vacutainer ( tu bes up to 1 32 rn m le ng th)
J) 4 microt1ter carriers witt1 2 plates in each
max.
speed
[rpm]
15000
15000
6000
3300
4000
---
ATTENTION -DANGERI The maximum speed of 2800 rpm must never be exceeded!
') sealedrotor -ATTENTION! Instructions in Section6.7 must be observed absolutely!
5) rectangle bu cketwith h erm etica ll y sealed li d
max. max.
RCF radius
[x g] [cm]
20120 8.0
2890 16.2
3490 16.9
1280 14 .6
17860 7.1
6240 15.5
3220
18.1
max. max.
RCF radius
(xg] [cm]
20120 8.0
17860 7.1
6240 15.5
1850 15.2
3360 18.8
--
max. Ioad
sit io nper po
[g]
3
5"10
425
360
4.9
510
510
-
max. load
per position
[g]
-3
4.9
510
260
800
ATTENTION -DANGER! Hermetical sealing is only guaranteed by troublefree operation of the centrifuge and its accessories!
Notice! The minimum reachable sample temperatures in MEGAFUGE 1.0R/2.0R/3.0R are equal to or towerthan 4°C for all rotors at maximum speed.
2.4.5 MEGAFUGE 3.0R Rotors
Rotors with Swing-out Buckets
rotor/bucket max. max.
Cat-No. capacity speed
[//] [ml] [rpm]
5315
1)
8X12X15
4700
80702) 4 carriers
2800
807 4 + 8078
3) 4 X
24 X 15
3500
807 4 + 8080
4) 4 X
750 3500
8074 + 8082
2 carriers 3300
1) sealed rotor -ATTENTION! Observe instructions
in Section6.7!
') rotor for mineral oil glasses (up to 200 mm length)
max. max. max. load
RCF radius per position
[xg] [cm )
[g)
4790 19.4 510
1910 21.8
260
2510 18.3 1020
2890 21 .1
1100
1960 16.1
260
ATTENTION -DANGER! When using the standard glasses #3 1 10 , # 311 1 and# 3112, the speed must be r ed uced to 1800rpm
3) double-rectangular
bu ck et
4) r
ou nd bucket with hermetically sealed lid
ATTENTION -DANGER! Hermetical sealing is only guaranteed by troublefree operation of the centrifuge and its accessories!
Notice! The minimum reachable sample temperatures in MEGAFUGE 1.0R/2.0R/3.0Rare equal to or lowerthan 4°C for all rotors at maximum speed.
11

3. SAFETY SYSTEMS
3.1 Rotor Chamber
MEGAFUGE 1.0/2.0
The rotor chamber consists ofa solid steel armor coated with enamel.
MEGAFUGE 1.0R/2.0R/3.0R
The stainlesssteel rotor chambe r is surrounded by a sol id armored vessel.
3.2 Lid Lock
The cabinet lid can only be opened when the rotor speed is at zero and the mains power on. The centr·ifuge
can only be started when the lid is correct ly locked and the "open" message has turned off.
3.3 Thermal Motor Protection
The power to the motor is switched off in the eventof motorovertemperalure to protect th e drive system.
MEGAFUGE 1.0R/2.0R/3.0R
An additional motor temperature switch turns on cooli ng fans to keep the temperature inside the un it a
suitable range.
3.4 ImbalanceSwitch
Al l MEGAFUGES are equipped with an imbalance switch.
If the all owab le imbalance is exceeded, the message "ba l" will alternate with th e speed display and the drive
brakes to standstill automatically (see 11.1).
3.5 Check of Acceleration Rate (only MEGAFUGE 1.0R/2.0R)
Whenever the rotor # 1379 or #304 1 is set, the microprocessor will check the acceleration rate to protect
the drive system or other rotor types from damagein the event of false programming (see 11.1, E-18).
12

3.6 Emergency Lid Release
ATTENTION -DANGER!
It is dangerous to open the lid while the rotor is spinning!
In the event ofa fa il ure of the electrical supply,the automatic lid release is rendered inoperative.
To remove the samples when the rotor has come to standsti ll, a manually operated re lease is provided.
Perfo rmance as fo llow s:
-look into lid window to ensure rotor is not spinning
-turn off the power, remove main power plug
-MEGAFUGE 1.0/1.0R/2.0/2.0R:
plastic stopper is localed to the rightcenter underthe front panel
-MEGAFUGE 3.0R:
plastic stoppers are located to the left and right of the rotor chamber under the front panel
-pry the stoppers fr om the un it using a flat bladed tool
-pu ll t he stoppers and it s attached cords downwards to unlock th e lid mec ha nical ly
Fig. 2
After openingpush the co rd back through the ho le and refit the plug.
3.7 Overtemperature Protection (only MEGAFUGE 1.0R/2.0R /3 .0R)
These models are equipped with a rotor overtemperature protection. If th e temperature inside the rotor
chamber exceeds 51 °C, tt1e microprocessor will indicate an overtemperature alarm (see 11.1, E-07).
13

4. INSTALLATION
4.1 Location
The centrifuges are delivered in a special cardboard or wooden box. Op en the box carefull y, remove th e
transportprotec ti on parts (foam) and put the un itm its location.
The unit must be set up on a sturdy, le vel laboratory t ab le or a suitable carriage with lockable wheels.
WARNING!
Do not place the unitnext to a heat emitting element or instrument!
There must be a minimum clearance to the wall and any adjacent equipment of 10 cm to ensure
unrestricted air circulation.
The international european regulations for centrifuges require a safety zone of 30 cm around the
centrifuge. We recommend to mark this area to keep away persons or dangerous goods (e.g. comprising
inflammable or infectious liquids} during centrifugation.
4.2 Electrical Requirements
Bef or e connecting th e Megafuge to the mains suppl y, make certain th at:
1. the line voltage imprinted on th e identification plate is equal wi th th e one available (see 2.3 Mains Supply}
2. th e line voltage circuit breaker is a 13, 15 or 16 A mp type with a slow release feature commonly used for
instruments.
4.3 Main Power Switch
Connect the line cord plug to the app r op riate wall socket. Turn on the main power sw it ch located on the back
left hand side. All displaysand diodeswill light up tor two seconds (system check).
ATTENTION!
The main power switch should not be us ed (except in case of emergency) to interrupt the centrifuga ti on . Fo r
t hat, the STOP key is provided.
Note: When powered off, all parameters of the last run a re main ta ined.
4.4 Opening the lid
The lid can only be opened with tr1c lid key when the yellow LED is li t. This is only possible when the rotor is
not turn in g and no er ro r messages arc displayed.
WARNING -DANGER!
The mechanical lid unlocking mechanism (see 3.6} must not be used to interrupt centrifugation. It is
absolutely necessary to wait until the drive has come to standstill (can be checked through the window
in the center of the lid).
14

5. ROTOR EXCHANGE
CAUTION!
Before inserting the rotor, make sure that the rotor chamber is free of contaminations (dust, debris etc.).
Condensate or residual sample liquid must be removed before centrifugation.
When clamping on the rotor to the drive shaft, the temperature of the rotor, the drive shaft and the collet
chuck must all be in the range of 10-30 °C to avoid loosening of the collet chuck by temperature
imbalance.
WARNING!
The rotor must be removed before transporting the centrifuge! Damage to the rotor and centrifuge may
result ifthe rotor is not removed prior to transporting.
5.1 Use of Collet Chuck
The rotor mustbe clampedfirmlyontothe drive shaft using a collet chuck (Cat.-No.4"70003100for MEGAFUGE
1.0/1.0R/2.0/2.0R and Cat.-No. // 70904041 for MEGAFUGE 3.0R).
The troublefree condition ofthe collet chuck must be checked before each rotor installation (see 10.3.).
Fi rs !, grease th e conical drive shaft (incl. th read) and the collet chuck slightly.
Place the collet chuck onto the drive shaft Ci> and twist it by hand at maxi mum 3 turns clockwise (see Fig. 3).
Fig. 3 rotor cham ber with correctlyattachedcol/et chuck
WARNING!
The collet chuck mustnotspread out (vanes ® must not open) yet.
Otherwise the rotor cannot be pushed down completely onto it's annular seat @ in which case co llet chuck,
rotor and centrifuge may be damaged!
15

5.2 Mounting
Seat the rotor gently onto the collet chuck and push it a ll the way down un til i ts bottom bears against the
annular seat@ofthe collet chuck.
Then use the 6 mm (or 10 mm for MEGAFUGE 3.0R) box spanner wrench to turn the hexagonal screw Ci)
(see Fig. 3) of the co llet chuck clockwise,thereby spreading the collet chuck untiltight.
Fi g. 4 Fastening the rotorclockwise
5.3 Removing
To remove the rotor, reverse the sequence.
CAUTION!
The collet ct1uck should always be removed -when not in use -so that it can't be damaged by inadve rt ently
starting without the rotor installed.
Fig. 5 Unscrewthe rotor counter-clockwise
16

}
6. ROTOR LOADING
WARNING!
The following instructions must be observed exactly to avoid damage!
When using the sealed rotor # 3360 or # 5315 , the loading instructi ons in Sec tion 6.7 must be obs erved
in every det ai l. All adapters must only be loaded with t ubes whi ch have the right shape , siz e and material
(observe tub e manufacturer recommendations).
6.1 Swing-out Rotors
WARNING!
In swing - ou t rotors all positions mus t be occupied with swinging bucke ts. The buckets may have an
ide nt i fi ca ti on letter which sta te s H1e weight class. (e. g. "E" or "D"). Only equal weight classes may be inserted
in opposi ng place s. Make sure that the tubes or adapters in opposing buckets are identica l.
6.2 Symmetrical Charge
All rotors must be loaded symme tr ically.Tubesand racks must be evenly distributed.
Following figures sh ow how 48 and 24 tubes can be correctly or in co rr ectly placed in a rotor wi th 48 places.
Fig. 6a
rotor fully loaded F
ig . 6b
ro tor sy mme tr ically loaded
a = be st solution
The tubes must always be distributed within the buckets in such a way that the roto r trnnnions are
stressed (loaded) evenly.
Fig. 6c
not allowed , because tr unni ons are
notevenly loaded
Fig . 6d
not allowed , because trunnions are
also not evenlyloaded
17

6.3 Distribution of Racks
In order to avoid rotor wobble which might cause damage to both lhe centrifuge and the sample, opposing
places must be provided with identically loaded adapters, multiple carriers, tubes, bottles etc . to obtain
the best possible balance.
Imbalance causes running no ises and produces a negative effect on th e cJrive system (excessive wear out of
the motorsupport).
The permiss i ble difference in total weight (or load) between opposing buckets is rotor dependent
(see RotorTables 2.4).
Todetermine the buc ket weights, any laboratory scale is suitable but a taring balance is best.
6.4 Tube Filling
Centrifuge tubes up to approx. 30 mrn in diameter may be fil led evenly by eye (up to 5-10 mm below the
rim, depending upon size).
NOTE!
Using an angle rot or and tubes without caps, only partial filling is possible (about 60% and 75% of the
maximum capacity). The actual amount of fil ling depends on the rotor's angle.
6.5 Partial Load
Wi th only a partial load, the tubes must be symmetrically distributed so that an even loading of the rotor is
achieved (see also Fig. 6b).
If a small number of sample tubes is to be centrifuged (less than the ful l capacity of the rotor), it is advisable
to obseNe the following rules for distribution ofthe tubes within the adapters.
Fig. 6e
correct loading
WARNING!
Fig. 6f
correctloading for tube length
> 100mm
To avoid glass brea kage wt1en using tubes greater than 100 mm in l ength, use the configurations shown in
Fig. 6f. In this case, always conducta manual swing-out test with emptytubes before committing samples.
18

6.6 Loading limits
rne material strength of every rotor Is limited with regmd to 1he mass and speed-dependent forces actrng on its
yoke a nd other c om ponents du ri ng centrifuge operation.
HERAEUS rotors are designed In such a way that they still possess high strength reserves at the maximum
perm1ss1ble load and speed. Still th e maximum permissible limi ts must not be exc ee ded . It is the operator's
responsibility to avoid overloa ding the rotor.
The max. permitted load is reached when all tubes are filled with a fluid of 1.2 g • cm-
3 density. When the
density is higher th;rn 1.2 the tubes may only be partly filled. The total weight of a bucket msy not exceed the
value corresµonding to to tal fi ll ing with a liquid of 1.2 dens i ty.
As an alternative in such cases, 11 is possible to reduce the maximum perrrntted speed nm;:ix in accordance
with the following formula:
✓ To:ai wc,y",I or ltw t•IIC:<ets rul s~m:il••
nadm = nmax w,tro a ,i,,ns,1y o: 1.2 9 cm -
---
l otdl vvei<Jht c: tt1H tJuckcts 11 c1 samp1t.?
w1 t ll h1ghnr c!e ns1 N
6.7 Sealed Rotors 3360/5315
ATTENTION!
Sp ecial lo ading instructions must be observed when us i ng the Seated Rotors# 3360 or # 5315!
The operating instructions which are supplied with the sealed rotors must al so be read and observed exactly!
The Sealed Rotor is a swing-out rotor with a sealing lid designed for applications involving infectious or
pathogenic samples. The centrifugal force of the buckets ,n this rotor Is absorbed by an exterior ring of
fiberglass-reinforced pla sti c. The lid reduces air friction whict1 a ll ows th is rotor to roach a high er max i mum
speed th an without lid.
Lock the rotor lid correctly to protect against spills due to broken glass or damaged tubes that may result 1n
aerosol cont am i na tion.
The buckets or rotor" 3360 or "' 5315 should be loaded wtlh Centn-Lab racks or inserts of identical exterior
dimensions and weight.
The rotor is intended to accomodato tubes 1 00 mm in length. However, shorter or longer tubes can be used.
Under certain circumstances, only the middle positions of the tube rack (sec fig. 6Q may be occupied by the
longer tubes to prevent them from being knocked against the rotor yoke. Check th at th e buckets swing out
fr eely.
Do not run the centrifuge with emptyglasstubes. Glass tubes of equal weight may exhibitverydifferent centers
ofgravity resulting in substan ti al im balance. This effect is reduced by filling th e tubes.
The unique co nstruction of the Sealed Rotor requires special precautions when operating. The following
rules must be absolutely observed in every detail.
WARNING!
It is esse nt ial thatthese rules are prec isely followed in order to preventdamage to the rotor or ce ntrifuge
resulting in a potential hazard to nearby equipment and personnel.
1) Attach the rotor co rrectly
2) Ensure each counter-weightis In itsownposition 1-4 (only for rotor~ 3360being balanced by theseweights)
3) Insert all buckets and all tube rack s. All buckets must be loaded.
4) Load bucketsevenly and symmetrically
5) Do not exceed permitted weight differences:
adjacent buckets: 100g
opposing buckets: 10 g
6) Do not exceed maximum sample load ( 51 0g per bucket)
7) Check troublcfree operation of th e rotor lid and observe expiration date (5 years laterthan imprinted date)
8) Lockrotor lid correctly
9) Do not exceed the speed limitof th e rotor (see RotorTables 2.4)
19

7. "MEGACONTROL" PROGRAMMING INSTRUCTIONS
A B C
11
I
10
I I 5 8 7 1
1i2
I I I
I I I I
~~:
/SOOD!
, 2 s
spud
ltm p
,er
-e~b<al<r -
starl· □ -
1
-- L_J
~o
I I I I
I I I I I I .
Fig. 7 1 2 3 4 6 9 I1\
14
MEGACONTROL - Key and Indication Board
Temperature displayis onlyexistanton MEGAFUGE 1 .0R/2 .0R /3.0R
A Display
B Display
C Display
1 "set" -key
2 "brake" -key
3 "set" -key
4 "set" -key
5 "+" -key
6 "-" -key
7 "lid" -key
8 "quick run"
9 "start stop"
10 red LED
11 yellow LED
12 green LED
13 green LED
14 red LED
20
for speed, RCF value, rotor type, brake cut -offpoint and special indications
for running tirne, "hd" (conti nuous opera ti on), "ro" (rotor type), "re" (RCF)
for temperature (MEGAFUGE 1.0R/2.0R/3.0R)
for speed- or RCFselecti on
a) toset brakecut-off point,
b) to switch between normal and sl ow acce leration
c) to sw i tch brake on/off
for running tim e- and rotor selection
for temperature sele ct ion (MEGAFUGE 1.0R/2.0R/3.0R)
to increase the selected value (auto-repeal)
to decrease the selected value (auto-repeat)
to open the lid
press-and-hold key for short cyc les, upon release, maximum braking occ u rs
dual functi on key for start and stop
a) on indicates normal acce leration
b) on indicates brake activated
on indicates lid may be opened
on indicates "quick run" may be used
on indicates run may be started (or restarted)
on indicatesrun may be terminated manual ly