Heraeus CLINIFUGE Instruction Manual

Heraeus
CLINIFUGE ™
Operating Instruction Manual
Kr LL

READ ME FIRST
The Clinifuge centrifuge is designed to perform a variety of separation tasks in small-scale laboratories. Before
operating, follow these few simple cautions and guidelines.
A FEW DO'S AND DON'Ts
Read all parts of this manual carefully before attempting to operate the unit, to insure smooth operation and
avoid damage to the unit or its accessories.
If a malfunction occurs, consult the Troubleshooting Guide at the end of this manual. If problems persist,
call Heraeus Instruments at 1-800-441-2554 or FAX information about the problem to Heraeus at 1-908-754-9494.
Never attempt to open the lid manually while the rotor is spinning!
Always use tube adaptors when loading 5 ml. and 7 ml. tubes (see Section 3.6). 10-, 12- and 15-ml. tubes
are loaded without adaptors.
Complete the warranty card shipped with your unit and return to the manufacturer.
FOR SAFETY, NEVER OPERATE THE CENTRIFUGE IF:
- the lid is open
— the rotor is not properly installed (see Section 3.5)
- the expiration date stamped on the rotor has passed (see Section 6.3) or the rotor has been used more than
10,000 cycles (see Section 6.2)
— the maximum weight of a tube including sample exceeds 24 grams or the density of the sample material
exceeds 1.2 g - cm~3 for top speed operation
— the rotor is not loaded symmetrically (see Section 4.1)
Never operate the Clinifuge if you have not checked the maximum centrifugal speed recommended by the
tube manufacturer.
WARNING! In case of imbalance (e.g. tube breakage or non-symmetrical loading), shut off power to the unit
immediately. To accomplish this:
1. press the STOP key on the front of the unit immediately
2. less preferably, pull the power plug.
NOTE: When performing centrifugation and other functions which may expose workers to splashed blood
or body fluids, all laboratory personnel must follow universal laboratory precautions. For details, contact
your local laboratory safety officer.

Clinifuge Centrifuge Operation Manual
(Revision 05/93)
READ ME FIRST
Table of Contents
FAST STARTUP REFERENCE GUIDE
Fig. 1. Clinifuge Control Panel
Fig. 2. Closed Clinifuge
Fig. 3. Opened Clinifuge
Fig. 4. Locking Nut and Assembled Rotor
Fig. 5. Tube Adaptors and Inserts (optional)
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3.2.
3.3.
3.4.
DESCRIPTION OF UNIT
Definition
Standard Parts
Optional Equipment
Mechanical Construction
Electrical Construction
Warranty
Operating Controls
Fig. 6. Clinifuge Operating Controls and Control Panel
Display Features
. Speed
. Time
. Standby Mode Light
SETTING UP THE CLINIFUGE
Location
Electrical Requirements
Self Check Cycle
Standby Mode
Automatic Lid Lock
Opening the Lid
Emergency Lid Opening
Accidental Lid Opening during Run
Fig. 7. Emergency Lid Opening
ROTOR REMOVAL AND INSTALLATION
Rotor Parts
Fig. 8. Fully Assembled Rotor
Fig. 9. Correct Rotor Installation
Fig. 10. Disassembled Rotor
Removing the Rotor
Taking the Rotor Apart
Fig. 11. Rotor Disassembly - Top View
Assembling the Rotor
Fig. 12. Assembling the Rotor
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3.5.
3.6.
5.2.
5.3.
5.4.
5.5.
5.5.1.
5.5.2.
6.1.
6.2.
6.3.
6.4.
6.5.
6.6.
7.
7.1.
7.2.
7.8.
7.4.
Installing the Rotor
Fig. 13. Seating Rotor on Drive Shaft
Fig. 14. Rotor Installation Procedure
Rotor Tube Adaptors
Fig. 15. Rotor Tube Adaptors with and without Inserts
Fig. 16. Tube Adaptors Loaded in Rotor
PROPER ROTOR BALANCING AND LOADING
Positioning Tubes for Symmetrical Balancing
Fig. 17. Correct Tube Positions
Positioning Tube Adaptors
Fig. 18. Loading Tube Adaptors into Rotor Top
OPERATING AND PROGRAMMING PROCEDURES
Opening the Lid
Fig. 19. Lid Key
Stop/Start
Fig. 20. Start/Stop Key
Quick Run
Fig. 21. Quick Run Key
Speed Selection
Fig. 22. Speed Selection Keys
Fig. 23. Speed Display
Time Selection
Timed Settings
Fig. 24. Time Selection Keys
Fig. 25. Time Display
Untimed Runs
Fig. 26. "hd" (hold) Time Display
MAINTENANCE
Cycle Counter Checkup
Fig. 27. Cycle Counter
Rotor Exchange
Rotor Expiration Date Stamp
Fig. 28. Expiration Date Stamp
Routine Cleaning Procedure
Decontamination Requirements
Shipping Requirements
TECHNICAL DATA
Electrical Line Supply
Rotor Characteristics
Performance Features
Environmental Requirements
TROUBLE-SHOOTING GUIDE
15
15
16
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17
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17
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18
19
19
19
19
19
19
20
20
20
20
21
21
21
21
21
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22
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23

FAST STARTUP REFERENCE GUIDE
Setting Up the Clinifuge
1. Set unit on a flat, sturdy, resonance-free surface in a well ventilated area.
2. After plugging into power supply, turn on the main power switch located under the left side of the unit
(See Electrical Requirements, Section 2.2.)
3. Open the lid by pressing the LID keypad.
4. Remove all packing materials. Rotor must move freely.
5. If adaptors are required, remove the rotor (See Section 3.6, Fig. 15), and insert adaptors (See Section 3.6,
Fig. 16)
6. Mount the rotor on the drive shaft, aligning the groove on the underside of the rotor base with the cross-pin
in the drive shaft. Screw locking nut in place. (See Section 3.5, Figures 13 and 14 for full rotor installation
instructions)
WARNING! If the locking nut does not easily engage the threaded portion of the drive shaft, do not try to
force the rotor further onto the drive shaft. Instead, remove the rotor and re-install, making
sure the rotor is correctly seated on the drive shaft cross pin.
7. Load tubes making sure the rotor is symmetrically balanced. (See Section 4.1, Fig. 17 on Positioning Tubes.)
8. Close lid making sure both lid locks are engaged. Display will read "OPEN” and centrifuge will not operate
if both locks are not fully engaged.
Programming the First Run
9. To set time, press the SET keypad in the TIME field. Change time by pressing "+" or "~" keypads.
10. To set speed for a timed run, press the SET keypad in the SPEED field. Change speed setting by pressing
"+" or "-" keypad.
11. For timed runs, press START keypad. Unit will shut off automatically.
For untimed runs, press QUICK START keypad and hold for desired time.
Preparing the Next Run
12. At end of cycle, remove all tubes from rotor.
13. Check for broken glass or leakage into rotor and/or chamber.
14. To remove rotor, remove locking nut by turning counter-clockwise. Insert index fingers into opposite tube
holes and lift rotor straight up. To prevent damage to drive shaft, do not lift rotor at an angle.
To prepare the next run, follow the routine cleaning procedure (Section 6.4), if necessary, then repeat Steps
3to 14.
For more detailed information about Clinifuge features, operation and guidelines, consult other sections of
this
manual.
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Figure 1. Clinifuge Control Panel
TO SET TIME:
Press SET keypad
in the TIME field.
Change time by
pressing "+" or "-" keypads.
TO SET SPEED:
Press SET keypad
in SPEED field.
Change speed ing by
pressing "+" or "-" keypad.
+/- KEYS
Use to change speed
and time settings up or down.
LID KEY
Press to open
Lid will pop up
automatically.
QUICK RUN KEY
Use for untimed runs.
Hold to start.
Release to stop.
START/STOP KEY
Press to start timed runs.
To stop anytime,
press START/STOP keypad.
Panel will display "br" message.

Figure 2. Closed Clinifuge
(Control Panel)
Figure 3. Open Clinifuge
(down view w/rotor mounted)
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==
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Figure 4. Locking Nut & Assembled Rotor
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Figure 5. Tube Adaptors & Inserts (optional)
6

1. DESCRIPTION OF UNIT
1.1. Definition
The Clinifuge is a laboratory centrifuge used for a variety of separation tasks in small-scale laboratories.
The following features provide easy, efficient operation:
— maintenance-free induction drive motor
— microprocessor controlled
— no carbon dust build-up due to elimination of carbon brushes
— membrane touch pads for easy cleaning
— CSA listed (Canadian Standards Association)
- angle rotor designed to carry 10-, 12- and 15-ml. tubes
(tube adaptors available as optional equipment for 5 ml. and 7 ml. tubes)
1.2. Standard Parts
The Clinifuge you have been shipped comes with the following parts:
1. Two-part rotor (Figure 4)
2. Locking Nut (Figure 4)
1.3 Optional Equipment
Tube adaptors and inserts (See Figure 5.)
1.4 Mechanical Construction
The Clinifuge frame is constructed of solid steel with an armor chamber and a suspension for the drive and lid
mechanics which provide extra security in the event of rotor malfunction. The impact-resistant plastic casing
is mounted to the frame to reduce noise and vibration. Digital displays (LED's - light emitting diodes) indicate
speed, time and operating conditions.
1.5 Electrical Construction
The Clinifuge operates on one circuit board using microprocessor-controlled digital LED displays and key
pads on the face of the unit to control program settings.
CAUTION: The microprocessor board is located directly behind the front panel. To avoid electric shock or
damage, only a factory trained technician may service this area.
1.6 Warranty
The Clinifuge is warranted against defects in materials and workmanship for a period of one year after shipment
to the customer.
The Clinifuge rotor is warranted against defects in materials and workmanship for the period of 10,000 cycles
or the expiration date stamped on the rotor, whichever occurs first.

1.7. Operating Controls
B ο D E Е
A. Speed Setting and Indication Field F. Start/Stop Key
B. Time Setting and Indication G. Indicator LED's
C. + and — Keys for Time and Speed Setting H. Main Power Switch (left side under base)
D. Lid Opening Key L Window
E. Quick Run Key J. Access holes (for manual and emergency lid opening)
Figure 6. Clinifuge Operating Controls and Control Panel
1.8. Display Features
1.8.1. Speed
Shows set speed during acceleration and centrifugation phases. When the unit brakes, "br" is indicated in the
speed field window.
1.8.2. Time
Records time in seconds up to one minute. Settings above one minute are counted down in full minutes, with
the last minute counted down in seconds,
1.8.3. Standby Mode Light
A red flashing dot in the time display field indicates the unit is in standby mode. (See Section 2.4, Standby Mode.)

2. SETTING UP THE CLINIFUGE
2.1. Location
Remove
packing
materials
and
place
the
centrifuge
on
a
sturdy,
resonance-free
table
in
an
area
allowing
good
air
flow
in
and
around
the
unit.
Proper
ventilation
is
required
to
keep
the
motor
cool
during
operation.
A
fan
built
into the
bottom
of the
centrifuge
also
helps
maintain
cool
operation.
Before
operating,
be
sure
to
remove
packing
materials
inside
the
unit
used
to
secure
and
protect
the
rotor
during
shipment.
To
open
the
unit,
you
will
need
to plug
it
in.
2.2. Electrical Requirements
Before connecting the unit to the main power supply, make certain that:
1. the line voltage imprinted on the identification plate is equal with the one available (120 Volts);
2.
the
line
voltage
circuit
breaker
is
a
15
Amp,
type
K,
slow
release
caracteristic
commonly
used
for
instruments.
A
main
power
switch
located
under
the
left
side
of
the
unit
may
be
used
to
turn
the
unit
on
and
off
as
desired.
(See
Figure
6.)
Once
the
power
supply
plug
is
connected,
the
main
switch
is
turned
on,
and
the
self-check
cycle
is
completed,
the unit
is
ready
for
operation.
2.3. Self Check Cycle
Each
time
the
unit
is
turned
on,
the
microprocessor
performs
an
automatic
self-check
of
the
electronics,
indicating
all
LED's
temporarily.
Whenever
the
centrifuge
is
disconnected
during
run,
the
unit
will
make
a
humming
sound.
When
the
self-check
is
complete,
the
yellow
LID
light
will
illuminate,
permitting
the
lid
to
be
opened.
2.4. Standby Mode
For
convenience,
the
Clinifuge
is
designed
to
operate
as
a
"constant
on.”
After
two
minutes
of
inoperation,
the
microprocessor
automatically
blanks
out all displays
and goes
into
a Standby
Mode,
indicated
by a flashing
red
LED
dot
in
the
time
display
field.
Press
any
key
to
reactivate
the
displays
and
to
perform
the
centrifugal
function.
2.5. Automatic Lid Lock
The
lid
is
all-steel
construction
with
an
integrated
strobe
port
and
tabs
on
both
sides
to
engage
the
automatic
dual
lid
locks.
2.6. Opening the Lid
To open the lid, turn the unit on and press the LID key pad. The lid will pop up automatically.
Three conditions will automatically prevent the lid from being opened:
1. The unit is not plugged into the electrical power source and/or the main switch is not turned on.
2. To prevent injury, the lid can not be opened while the rotor is spinning. Wait until the rotor comes to a full
stop before attempting to open the lid. This may be checked by looking through the window in the lid.
3. If a power failure occurs during operation, the lid will remain locked until power returns. In case of power
failure,
the
centrifuge
always
performs
a
complete
braking
cycle.
When
power
is
restored
to
the
centrifuge,
the “br” code will be displayed accompanied by a buzzing sound. To re-start after braking cycle is
completed, press key pad for desired function. (Refer to Emergency Lid Opening instructions in Section 2.7.)
NOTE:
Time
and
speed
settings
are
retained
in
memory
and
do
not
need
to
be
re-entered
after
power
failure
or
between
operating
cycles.

2.7. Emergency Lid Opening
WARNING! Never attempt to open the lid manually while the rotor is still spinning.
Confirm that rotor has stopped spinning by looking through the round window in the middle
of the lid.
Disconnect power cord before using the following Emergency Lid Opening Procedure.
2.8. Accidental Lid Opening during Run
If the lid opens during a run, the microprocessor will flash the LID error message. Before lifting the lid, confirm
that the rotor has come to a full stop by looking through the round window in the middle of the lid.
In case of a prolonged power failure or defect, the LID button can not be used to open the lid. The locked
cabinet lid can be opened manually as follows:
1. Disconnect the power cord before using the emergency lid opening procedure.
2. Make sure the rotor is fully stopped by looking through the round lid window.
3. Insert two rods into the access holes on both side panels (See Figure 7) and unlock the lid, one side
ata time.
Access
Hole
Access Hole
Figure 7. Emergency Lid Opening
Not to be used while rotor is still spinning!
10

3. ROTOR REMOVAL AND INSTALLATION
The Clinifuge rotor is shipped installed in the centrifuge and secured for shipping.
WARNING! Before operating the centrifuge, remove all packing material placed inside the unit for shipping.
To operate, the rotor and drive shaft must turn freely and the rotor nut must be checked for tightness.
by turning clockwise. For safety in operation, check the locking nut for tightness before each run
Each time the rotor is re-installed, the rotor and chamber must be completely free of contamination
(dust, glass splinters, etc.).
To insure proper operation and prevent damage, the rotor must be properly positioned on the
specially formed drive shaft each time it is re-installed. (See Section 3.1, Fig. 9 and Section 3.5.)
3.1. Rotor Parts
The rotor is a two-piece unit connected to the drive shaft by a locking nut.
Nut
Rotor Nut
Rotor Top
Figure 8. Fully Assembled Rotor
É Groove on < Rotor Base
underside of (right side up)
G O rotor base
RC
Threaded portion
NZ of drive shaft
Cross-pin
groove
Drive shaft =
4
cross
pin
Rotor
Base
(Bottom view)
Figure 9. Correct Rotor Installation Figure 10. Disassembled Rotor

3.2. Removing the Rotor
To remove the rotor from the chamber:
1. Open the lid.
2. Remove all tubes and check for broken glass.
3. Remove rotor locking nut by turning counter-clockwise.
4. To remove rotor, insert index fingers into holes in rotor and pull straight up. If properly installed, the entire
rotor will lift out. (Before inserting fingers, check holes for broken glass.)
WARNING! Never pull the rotor up at an angle. Doing so could bend the drive shaft and cause permanent
damage to the motor.
3.3. Taking the Rotor Apart
To separate the rotor top from the rotor base:
1. Remove rotor from unit and place on a flat surface.
2. Insert index fingers into empty tube holes on opposite sides of the rotor top.
3. Using thumb, press firmly down on centerpiece. The two halves of the rotor should separate easily.
Rotor Hole
Centerpiece
Press here
to separate
Rotor Base
Figure 11--Rotor Disassembly — Top View

3.4. Assembling the Rotor
1. Before each installation, clean the chamber and rotor of dust, glass splinters and any other materials.
2. Insert tube adaptors, if required. (See Section 3.6, Rotor Tube Adaptors and Section 4., Proper Rotor
Balancing and Loading.)
3. To assemble, line up the tab in the center hole of the rotor top with the corresponding notch in the rotor
base centerpiece. Push down gently until the unit snaps together. The center of the base will be slightly
recessed.
Alignment notch Alignment tab in
in centerpiece of center opening
rotor base of rotor top
Figure 12. Assembling the Rotor
3.5. Installing the Rotor
A slot matching the cross pin on the drive shaft is grooved into the underside of the rotor base and must be
aligned precisely when installing the rotor in the chamber.
WARNING! Position the rotor so that the slot grooved into the underside of the rotor base seats in exact
alignment with the cross pin on the drive shaft. Failure to do so will prevent proper seating.
1. Turn the drive shaft to place the cross pin in a known position, e.g. long bar pointing to front (See Figure 9).
2. Using the tab in the rotor centerpiece as a guide, position the grooved notch on the underside of the rotor
base in exact alignment with the cross pin on the drive shaft. Press firmly into place.
3. When rotor is properly seated, secure the locking nut, turning clockwise.
NOTE! If the locking nut does not easily engage the threaded portion of the drive shaft, do not try to force
the rotor further onto the drive shaft. Instead, remove the locking nut and the rotor, and re-install, making
sure that the the rotor is properly seated. (To insure correct seating, be sure Step 4 2 is properly
performed.)
RIGHT WRONG
(threaded portion extends (Threaded portion
completely above rotor below rotor
centerpiece centerpiece)
Figure 13. Seating Rotor on Drive Shaft
13

Figure 14. Rotor Installation Procedure
3.6. Rotor Tube Adaptors
The Clinifuge-rotor is designed to hold 5 ml., 7 ml., 10 ml., 12 ml. and 15 ml. tubes.
Tube adaptors and inserts (Section 1.3, Fig. 5) are available as optional equipment and tubes must be
positioned as follows:
- 10, 12 and 15 ml. tubes may be placed directly into the rotor without adaptors
- 7 ml. tubes must be loaded into adaptors without inserts before being positioned in the rotor (See Figure 16)
- 5 ml. tubes must be loaded into adaptors which have been fitted with inserts before being positioned into the
rotor (See Figure 15)
Rh — Top |
Tab Tab Insert
(7 ml. tubes) 會 (5 ml tubes)
Rotor Tube Adaptor without Insert Inserts A Î Rotor Tube Adaptor with Insert
Figure 15. Rotor Adaptors with and without Inserts
Figure 16. Tube Adaptors Loaded in Rotor
14

4. PROPER ROTOR BALANCING AND LOADING
WARNING! The rotor must always be symmetrically balanced when loading.
In case of imbalance (caused by tube breakage or non-symmetrical loading), press the STOP key
immediately, pull the plug or turn off circuit breaker.
To maintain symmetrical balancing, insert tube adaptors in all empty opposing slots.
4.1. Positioning Tubes for Symmetrical Balancing
For
correct
balance
in
loading
the
rotor,
tubes
and
adaptors
must
be
placed
exactly
opposite
tubes
(and
adaptors) of equal size and weight.
The maximum allowable difference in weight between opposing tubes (and adaptors, if used) is one gram.
Glass tubes have different wall thicknesses and, therefore, hold different volumes. This must be taken into
consideration when positioning tubes in the rotor.
CAUTION!
G-forces
vary
from
rotor
to
rotor.
To
prevent
breakage,
observe
tube
manufacturer
recommendations.
Tubes
made
of Polystyrol tend to break
at very
low g-forces.
Empty positions Filled positions
Figure 17. Correct Tube Positions
NOTE: When using either flared top, 12-ml. or 15-ml. tubes, load symmmetrically in every other position
for proper balance. (See # 3)

4.2. Positioning Tube Adaptors
Tube adaptors for 5 ml. tubes and 7 ml. tubes must be installed before loading tubes into the rotor.
To position adaptors:
1. Before loading, separate the two halves of the rotor. (See Section 3.3)
2. Turn the rotor top upside down and place on a flat surface.
3. For 7 ml. tubes, insert each adaptor pair pointing the flat ends down with the two tabs turned extending into
the center of the bowl. Secure each adaptor by pushing firmly into the rutted sleeves
For 5 ml. tubes, push the flat end of the inserts into the curved openings in the adaptor, sliding the side tabs
on the insert into the grooves in the adaptor. Follow #3 to position the adaptor inside the rotor.
To remove inserts, push a pencil or similar tool through the open end in the adaptor.
WARNING! When loading adaptors, opposite sides of the rotor must be equally balanced in weight.
(See Section 4, Proper Rotor Balancing and Loading.)
Figure 18. Loading Tube Adaptors into Inverted Rotor Top
16

5. OPERATING AND PROGRAMMING PROCEDURES
5.1. Opening the Lid
Figure 19. LID Key
5.2 Start/Stop
Figure 20. START/STOP Key
The lid can be opened only when the braking
process is completed and the yellow LID LED goes
on. To open, press the LID key pad. The lid will pop
up automatically.
Once the unit starts, the LID key is disabled for the
entire operating cycle.
NEVER TRY TO OPEN THE LID WHILE ROTOR IS
TURNING. (See Emergency Lid Opening Pro-
cedure.)
NOTE: In case of power failure, the LID key pad
cannot be used to open the lid. Follow Emergency
Lid Opening Procedure, Section 2.7, Fig. 7.
When the green START LED is on, centrifugation
may be started by pressing the START/STOP key
pad. When the rotor starts turning, the green
START/STOP LED goes off and the red LED goes on,
indicating the STOP function is available.
When the cycle is completed and the braking cycle
has ended, the green START LED and yellow LID
LED will both illuminate, indicating that the unit may
be opened or another cycle may be started.
To stop centrifugation, press the START/STOP key
pad when the red LED is illuminated.
After each stop, the green START LED lights up
again, indicating that the interrupted cycle may be
continued at any time. If START is pressed during
the braking cycle there will a delay before the Clini-
fuge begins to accelerate.
17

5.3. Quick Run
Figure 21. Quick Run Key
5.4. Speed Selection
IAN
Figure 22. Speed Selection Keys
Figure 23. Speed Display
If the green START LED is on, centrifugation may
also be started with the QUICK RUN key. To operate
in QUICK RUN mode, press and hold the QUICK
RUN key for the required length of time. While the
QUICK RUN key is pressed, the rotor accelerates
gradually to its highest speed. Centrifugation
continues until the key is released, which causes
the rotor to brake immediately.
If QUICK RUN is pressed again, acceleration will
be re-activated.
The QUICK RUN key may be used for runs requiring
less than one minute. (Braking will still require the
same time.)
The QUICK RUN key may also be used to override
the speed set in programming)
Speed is adjusted in gradations of 100 revolutions
per minute.
Speed settings are retained in the unit's memory
during standby periods, and need be entered only if
changing the previously set value.
When the centrifuge is in Standby Mode, the display
screens for TIME and SPEED are blank. To activate,
press the SET key pad in the SPEED field. The
previously set speed will appear, flashing the digit in
the 100ths position in the speed display window.
Set the new speed up or down using the "+" or
"=" keys.
After entering the new speed, press the SET key pad
in the SPEED field again to replace the previously
set speed in memory. The speed setting mode is
complete.
18