Hermle Z 206 A User manual

© Hermle Labortechnik GmbH Z206A_V1.18_eng
Operating Manual for
Small Centrifuge
Z 206 A

CONTENTS
© Hermle Labortechnik GmbH Z206A_V1.18_eng I
1. PRODUCT DESCRIPTION ..................................................................................................1
1.1 Safety Instructions ............................................................................................................................................ 1
1.2 Intended Purpose .............................................................................................................................................. 1
1.3 Brief Description................................................................................................................................................ 1
1.4 Delivery Package ............................................................................................................................................... 1
1.5 Operating and Display Elements...................................................................................................................... 2
1.5.1 LCD-Display .................................................................................................................................................... 2
1.6 Signs and Indications of the Centrifuge .......................................................................................................... 3
1.6.1 General............................................................................................................................................................ 3
1.6.2 Product Nameplate (Example) ........................................................................................................................ 3
1.6.3 Warning and Information Signs ....................................................................................................................... 4
1.6.4 Declaration of ATEX (94/9/EG) ....................................................................................................................... 5
1.6.5 Following Rules:.............................................................................................................................................. 5
1.6.6 Warranty.......................................................................................................................................................... 6
1.7 Installation of the Centrifuge ............................................................................................................................ 6
1.7.1 Unpacking the Centrifuge................................................................................................................................ 6
1.7.2 Space Requirements....................................................................................................................................... 6
1.7.3 Installation ....................................................................................................................................................... 6
1.8 Basic Adjustment .............................................................................................................................................. 7
1.8.1 Adjustment of the Rotor Type.......................................................................................................................... 7
1.8.2 Access to the Mode: "Operating Data"............................................................................................................ 8
1.8.3 Retrieving Operating Data............................................................................................................................... 9
2. OPERATION......................................................................................................................10
2.1 Mounting and Loading the Rotor ................................................................................................................... 10
2.1.1 Installation of the Rotor.................................................................................................................................. 10
2.1.2 Loading Angle Rotors.................................................................................................................................... 11
2.1.3 Loading Swing Out Rotors............................................................................................................................. 11
2.1.4 Loading and Overloading of Rotors............................................................................................................... 12
2.1.5 Removing the Rotor ...................................................................................................................................... 12
2.2 Power Switch ................................................................................................................................................... 12
2.3 Lid ..................................................................................................................................................................... 13
2.3.1 Lid Release ................................................................................................................................................... 13
2.3.2 Lid Lock......................................................................................................................................................... 13
2.4 Pre-Selection.................................................................................................................................................... 14
2.4.1 Pre-Selection of Speed and RCF .................................................................................................................. 14
2.4.2 Pre-Selection of Running Time...................................................................................................................... 15
2.4.3 Pre-Selection of Brake Intensity and Acceleration......................................................................................... 15
2.5 Starting and Stopping the Centrifuge............................................................................................................ 16
2.5.1 Starting the Centrifuge................................................................................................................................... 16
2.5.2 The "STOP" Key............................................................................................................................................ 17
2.6 Imbalance Detection........................................................................................................................................ 17
3. MAINTENANCE.................................................................................................................18

CONTENTS
II Z206A_V1.18_eng © Hermle Labortechnik GmbH
3.1 Maintenance and Cleaning.............................................................................................................................. 18
3.1.1 General Care:................................................................................................................................................ 18
3.1.2 Cleaning and Disinfecting of the Unit ............................................................................................................ 19
3.1.3 Cleaning and Disinfecting of the Rotor.......................................................................................................... 19
3.1.4 Disinfection of Aluminium - Rotors ................................................................................................................ 19
3.1.5 Disinfection of PP-Rotors .............................................................................................................................. 19
3.1.6 Glass Breakage............................................................................................................................................. 20
3.2 Service Life of Rotors, Round and Rectangular Buckets, accessories...................................................... 20
4. TROUBLE SHOUTING ......................................................................................................21
4.1 Error Messages: Problem / Solution .............................................................................................................. 21
4.2 Survey of Possible Error Messages and Solutions...................................................................................... 21
4.2.1 Lid Release during Power Failure (Emergency Lid Release)........................................................................ 21
4.2.2 Description of the Error Message System..................................................................................................... 22
5. REPAIR..............................................................................................................................22
6. TRANSPORT, STORAGE, DISPOSAL .............................................................................23
6.1 Transport.......................................................................................................................................................... 23
6.2 Storage ............................................................................................................................................................. 23
6.3 Disposal............................................................................................................................................................ 23
7. APPENDIX...........................................................................................................................II
EC Conformity Declaration...................................................................................................................................... III
Table 1: Technical Data ..........................................................................................................................................IV
Table 2: Permissible Net Weight..............................................................................................................................V
Table 3: Max. Speed and RCF-Values for Permissible Rotors ................................................................................V
Table 4: Acceleration and Deceleration Times.........................................................................................................V
Table 5: Error Messages.........................................................................................................................................VI
Table 6: Symbol / Abbreviations..............................................................................................................................VI
Notes.......................................................................................................................................... I

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z206A_V1.18_eng 1
1. PRODUCT DESCRIPTION
1.1 Safety Instructions
This symbol indicates safety instructions and points of potential dangerous situations. Before using
the centrifuge for the first time, please read the operating manual.
Failure to follow these instructions can result in personal injury and/or property damage .
Intended use includes: the observation of all instructions, in the instruction manual, and
administering inspection and maintenance.
1.2 Intended Purpose
This Hermle centrifuge was designed only for the separation of materials or mixtures with different
densities, specifically for the preparation and processing of samples, from the human body, in
context of an in-vitro-diagnostic use, to allow the use of in-vitro-diagnostic in accordance to its'
intended purpose. The designated device and its' accessories listed, in the technical documentation,
are in compliance with Directive 98/79/EC for In-Vitro-Diagnostic Medical Devices.
Hermle Centrifuges are intended exclusively for indoor use and for the use of qualified personnel.
Only Hermle original rotors and accessories should be used. Any other use or intended use is strictly
prohibited. For any resulting damage, the company, Hermle Labortechnik, is not liable.
1.3 Brief Description
The unit type Z 206 A is a non refrigerated universal centrifuge, which we offer in two voltage
variations 230V or 120V.
The centrifuge can be used with swing-out and angle rotors.
All parameters are accessible via buttons and selected with the potentiometer. All pre-selected and
current values will be shown permanently on the LCD-display.
The centrifuge is powered by a maintenance-free brush motor.
Detailed technical data are in "table 1 Technical data" (see APPENDIX S.IV).
1.4 Delivery Package
•1 Centrifuge Z 206 A
•1 Instruction Manual Z 206 A

PRODUCT DESCRIPTION
2Z206A_V1.18_eng © Hermle Labortechnik GmbH
1.5 Operating and Display Elements
1potentiometer Run Parameters
3LCD Control Panel Display
4rpm/rcf Speed/ g-force
5 accel/decel Acceleration/Deceleration Intensity
6time Centrifugation Time
7 lid Lid Release
9start Start Centrifugation
10 stop Stop Centrifugation
1.5.1 LCD-Display
The following picture shows the individual elements of the LCD-Display.
Display Fields:
A-1 Display Field –"rpm/rcf"
A-2 Display Field –"acc/dec"
A-3 Display Field–"time"

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z206A_V1.18_eng 3
Messages/Logo of the Display Field:
M1 "close" M6 "rcf"
M2 "open" M7 "accel"
M3 "rotor" M8 "decel"
M4 Rotor-No. M11 "error"
M5 "rpm" M12 "service"
1.6 Signs and Indications of the Centrifuge
1.6.1 General
Instructions for Disposal (see Chapter 6, S. 23)
Direction of Rotation –clockwise rotation for the rotor drive
Reference for Loading Rotors
1.6.2 Product Nameplate (Example)
Company Address: Hermle Labortechnik GmbH, Siemensstr. 25, D-78564 Wehingen
TYPE: Type Designation of the Product
REF: Order No. of the Product
SN: Serial Number of the Product
Date of Manufacture
Manufacturer
MAX. SPEED: Max. Allowed Speed of the Unit
KIN. EN.: Max. Kinetic Energy with Corresponding Rotor

PRODUCT DESCRIPTION
4Z206A_V1.18_eng © Hermle Labortechnik GmbH
U/I/f: Allowable Voltage / Max. Current / Frequency
P: Electrical Input Power
Operating Manual
RoHS-Compliance
Labeling, Standards and Guidelines
1.6.3 Warning and Information Signs
Four carrier must be used at all times on four place swing out rotors or
damage will occur to the centrifuge. Such damage will not be covered
under the product warranty.
Attention! Check the fastening of the rotor nut before each run.
Take off mains plug before opening the housing or the emergency
release
Power Input
This device may only be operated by a trained professional. Carefully, read the
operating manual and be familiar with the functions of the device.

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z206A_V1.18_eng 5
1.6.4 Declaration of ATEX (94/9/EG)
The HERMLE Z 206 A is not "explosion-proof" and must therefore not be operated in explosion-
endangered areas or locations. During centrifugation, it is prohibited to stay within the safety zone of
30 cm/12in around the centrifuge or deposit hazardous substances, within this area. Centrifugation
of flammable, explosive and radioactive substances or substances, which chemically
react with high energy, is strictly prohibited! If used in such environment, this is at the users own
expense.
•Never spin toxic or pathogenic material without adequate safety precautions, i.e.
centrifugation of buckets / tubes with or without defective hermetic sealing, is strictly
prohibited. The user is obliged to perform appropriate disinfection procedures, in case
dangerous substances have contaminated the centrifuge and/or its' accessories. When
centrifuging infectious substances, always pay attention to the General Laboratory
Precautions. If necessary, contact your safety officer!
•It is prohibited to run the centrifuge, with rotors not manufactured for this unit.
•Under no circumstances open the lid of the centrifuge, while the rotor is still running or
rotating with a speed of > 2m/s.
This device may only be operated by a trained professional. Carefully, read the operating
manual and be familiar with the functions of the device.
1.6.5 Following Rules:
•Do not operate the centrifuge if not installed correctly.
•Do not operate the centrifuge when dismounted (e.g. without housing).
•Do not run the centrifuge, if mechanical or electrical assembly groups have been tampered
with, by unauthorized personnel.
•Do not use accessories such as rotors and buckets, that are not approved by HERMLE
Labortechnik GmbH, except commercially available centrifuge tubes, made of glass or
plastic.
•Do not spin extremely corrosive substances, as they may cause material damages and impair
mechanical resistance.
•Do not operate the centrifuge with rotors or buckets, that show any signs of corrosion or
mechanical damage.
The manufacturer is responsible for safety and reliability, of the centrifuge, only if:
•The unit is operated in accordance to this instruction manual.
•Modifications, repairs or other adjustments are performed by HERMLE-authorized personnel
and the electrical installation of the related location corresponds to the IEC-regulations.

PRODUCT DESCRIPTION
6Z206A_V1.18_eng © Hermle Labortechnik GmbH
1.6.6 Warranty
The centrifuge has been subjected to thorough testing and quality control. In the unlikely case of any
manufacturing faults occurring, the centrifuge and rotors are covered by warranty, for a period of two
years, from date of delivery. This warranty becomes invalid in any case of mishandling, damage
and/or negligence and further in any case of usage of inappropriate spare parts and / or accessories
or unauthorized modification of the unit.
Technical modification rights are reserved, by the manufacturer, in regards to
technical improvement!
1.7 Installation of the Centrifuge
1.7.1 Unpacking the Centrifuge
Model Z 206 A is supplied in a carton.
Remove the strap retainer, open the carton, and remove the centrifuge. The instruction manual must
be kept with the centrifuge, at all times!
1.7.2 Space Requirements
The centrifuge should be installed on an even, solid surface, if possible on a laboratory cabinet /
table or some other solid vibration free surface.
During centrifugation, the centrifuge must be placed in a way, that there is a minimum space of
30 cm/11.81in on each side of the unit, according to EN 61010-2-020 standards.
Do not place the centrifuge next to a window or a heater where it could be disposed to excessive
heat, as the performance of the unit is based on an ambient temperature of 23°C/73.4°F.
1.7.3 Installation
Follow these steps:
•Check whether the power supply corresponds with the one specified on the manufacturer's rating
label, mounted on the rear panel.
•The line voltage circuit breaker is max. 10 A (Type K), slowly release for commonly used
instruments.
•In case of emergency, there must be an emergency switch off installed outside of the room, in order
to disconnect the power supply from the unit.
•Connect the centrifuge, with the mains.
(The socket for the power cord must be easy to reach, respectively easy to disconnect).
-Switch on, by using the mains power switch (2.2).
Open the lid, by using the button LID.
• Remove the transport securing device of the motor.

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z206A_V1.18_eng 7
1.8 Basic Adjustment
1.8.1 Adjustment of the Rotor Type
Before the first operation resp., after each rotor change, choose the rotor type needed.
Find the rotor type on the printed order number, on the rotor.
Example:
Angle Rotor: 220.96 V01 rotortype "96"
Swing-Out Rotor:220.68 V04 rotortype "68"
Switch on the unit and open the lid. Press simultaneously, the keys “lid“ (7) and “stop” (10). In the
display, “rpm/rcf” (A-1) appears the old previous rotor type, i. e. “96”. With the potentiometer (1),
choose the used rotor now, (see Figure 1). To select the data in the unit, please press the “start” (9)
key.
*Now all important rotor parameters are stored in the centrifuge.
Figure 1
Attention: Set rotor must always match with the used rotor, otherwise the unit can be
damaged.
The rotor type can be checked during the run by pressing the key "lid" (7).

PRODUCT DESCRIPTION
8Z206A_V1.18_eng © Hermle Labortechnik GmbH
1.8.2 Access to the Mode: "Operating Data"
In this model, the following points can be reviewed:
-Number of Starts
-Operating Hours of Centrifuge
-Operating Hours of Motor
-Software Version
-Error List
-Function of the Imbalance Switch
-Operation of Keyboard
-Display Tests
If the centrifuge is still turned off, press the keys, "time" (6) and "lid" (7) simultaneously, turning on
the main switch of the centrifuge. Now release both keys. As a result, a display test is administered
for approx. 5 seconds. All possible indications will appear at the same time (see Figure 2).
Figure 2

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z206A_V1.18_eng 9
1.8.3 Retrieving Operating Data
In the function, "Basic Adjustments" the operating data, of the centrifuge, can be retrieved.
Please proceed as described, under point 1.8.2, to enter this program mode.
Press the key "accel/decel" (5). In the display, "accel/decel" (A-2) flashes the word, "service".
With the potentiometer(1) the following information can be retrieved:
A = Previous Starts of the Centrifuge
H = Previous Operating Hours
S = Software Version
E = List of Previous Error Messages
h = Running Time of the Motor
The list of the last 99 error messages can be looked over by pressing the key „rpm/rcf“ (4) and leaf
through it by the potentiometer(1). The respective error codes appear in the display „rpm/rcf“ (A-1)
(see figure 3). The first two numbers indicate the appeared errors ongoing from 00 to 99, the last two
numbers indicate the error code. Please look up in "table 5: error messages" (See APPENDIX VI).
Switch off the centrifuge, to change back to the normal program mode.
Figure 3

OPERATION
10 Z206A_V1.18_eng © Hermle Labortechnik GmbH
2. OPERATION
2.1 Mounting and Loading the Rotor
2.1.1 Installation of the Rotor
Clean the drive shaft and the collet with a clean, grease-free piece of cloth. Place the rotor onto the
drive shaft, (see Figure 4). Please be sure that the rotor is fully installed onto the motor shaft)
Please be sure that the rotor shaft is completely plugged into the rotor slot, (see Figure 5).
Figure 4 Figure 5
Figure 6
Hold the rotor with one hand and secure the rotor to the shaft, by turning the fixing screw clockwise,
(see Figure 6).
ATTENTION:
For safety reasons, check the fixing screw before each run.
Do not operate the centrifuge with rotors or buckets, that show any signs of
corrosion or mechanical damage.
Do not operate with extremely corrosive substances, that could damage the rotor,
buckets and materials.
In case of any questions, please contact the manufacturer!

OPERATION
© Hermle Labortechnik GmbH Z206A_V1.18_eng 11
2.1.2 Loading Angle Rotors
It is recommended to operate, e.g. a 12-place-rotor, with 2, 4 or 8 loaded tubes only.
But the loaded borings must be opposite of each other, (see Figure 8).
Figure 7 :WRONG Figure 8: RIGHT
Rotors must be loaded symmetrically and with equal weight, (see Figures 7 and 8). The adapter may
only be loaded with the appropriate vessels. The weight differences between the filled vessels
should be kept as low as possible. Therefore, we recommend to weigh with a balance. This reduces
the wear of drive and the acoustic operating noise.
2.1.3Loading Swing Out Rotors
Loading of the buckets / vessels must be in accordance to, Figure 10
It is recommended to operate, e.g. a 6-place swing out rotor, with 4 loaded buckets only. But, the
loaded buckets must be opposite of each other. Make sure that the unloaded buckets are placed
inside the rotor.
In principle, swing out rotors should not be used in operation, until all buckets or racks are
placed inside the rotor.
ATTENTION:
Swing Out Rotors can be removed during operation, only if all locations are filled in with either
4 buckets or 4 carriers –do not mix up buckets and carriers!!
Figure 9: WRONG Figure 10: RIGHT

OPERATION
12 Z206A_V1.18_eng © Hermle Labortechnik GmbH
2.1.4 Loading and Overloading of Rotors
All approved rotors are listed with their maximum speed and maximum filling weight, in
Table 2: "Permissible Net Weight", (see APPENDIX P. V).
The maximum load permitted for a rotor is determined by the manufacturer, as well as the maximum
speed allowed for this rotor (see label on rotor), must not be exceeded. The liquid the rotors are
loaded with, should have a max. homogeneous density of 1.2 g/ml or less when the rotor is running
at maximum speed.
In order to spin liquids with a higher density, the speed has to be reduced, according to the following
formula:
Reduced Speed nred =
 x max. speed (nmax) of the rotor
Example:
nred = 
 x 4.000 = 3.360 rpm
In case of any questions, please contact the manufacturer!
2.1.5 Removing the Rotor
Completely, untighten the rotor fixing nut (2. screw over the stiff point) and lift the rotor vertically out
of the centrifuge, (see Figure 6).
2.2 Power Switch
The power switch is located on the left-hand side of the ground plate, on the unit, (see Figure 11).
Figure 11: Power Switch
ATTENTION:
After turning on the power switch, open the centrifuge lid first, before starting the centrifuge.

OPERATION
© Hermle Labortechnik GmbH Z206A_V1.18_eng 13
2.3 Lid
2.3.1 Lid Release
After the run, properly close the lid of the centrifuge, appearing in the display, "rpm/rcf"(A-1) with the
word, "close" (M1). If there is a rotor in the centrifuge, the word, "rotor" (M3) appears, as well as the
code number of the specified rotor, which is in the centrifuge, for example "221.28" (M4). If there is
no rotor in the centrifuge, it flashes the word, "rotor" (M3) and an additional word, "no" (M4). ). By
pressing the key, "lid" (7), the lid of centrifuge can be released. As soon as the electromagnetic lid is
completely released, the word, "open" (M2) appears. The lid of the centrifuge is now able to be
opened.
For all number marked text, please refer to Figure 12.
Figure 12
2.3.2 Lid Lock
The lid must only be closed slightly. An electromagnetic lid lock closes the lid, the word "open" (M2)
will no longer be displayed. As a sign that the centrifuge is ready for starting, appearing in the
display, "rpm/rcf"(A-1), the word "close" (M1). Simultaneously, the word "rotor" (M3) appears, as
well as the code number of the rotor, which is in the centrifuge, i. e. „nr 96“ (11) along with all rotor
specific data, for example: max. speed, acceleration etc., are available, (see Figure 12).
ATTENTION
Before closing the lid, please check if the rotor is tightened, and all 6 buckets are inside the
swing out rotor.

OPERATION
14 Z206A_V1.18_eng © Hermle Labortechnik GmbH
2.4 Pre-Selection
2.4.1 Pre-Selection of Speed and RCF
By Selecting the key, "rpm/rcf" (4), pre-selection is activated. By pressing the key once, the word
"rpm" (M5) flashes. By pressing the key again, the pre-selection of the centrifugal forces can be
chosen. The flashing word, "rcf" (M6), will appear.
The desired values can be selected, with the adjustable knob (1). In the display (A-1), the regulated
value is shown permanently: before, during and after the run.
This pre-selected value will be stored, as long as a new pre-selection is made.
For all number marked text, please refer to Figure 13.
Figure 13
The speed is adjustable between 200 rpm and the maximum revolution of the centrifuge resp. of the
maximum permissible revolution, of the pre-selected rotor.
The pre-selection, of the RCF-value, is the same value. The setting range is between 20 xg and the
maximum permissible centrifugal force, of the rotor.
The maximum speed of the Z 206 A is 6,000 rpm resp. 4,427 xg.
Please refer to: "Table 3: Max. Speed and RCF", (See APPENDIX V). This table shows the
approved rotors with their max. permitted speed and RCF values.
ATTENTION:
Please notice the maximum permissible revolutions of the test tubes!!, (Producer Indication).
Please refer to: Chapter 3.1.6, for more information.

OPERATION
© Hermle Labortechnik GmbH Z206A_V1.18_eng 15
2.4.2 Pre-Selection of Running Time
The running time can be pre-selected in 3 different ranges: from 10 seconds up to 99 hours 59
minutes.
1. Range from: 10 seconds up to 59 minutes 50 seconds, in steps of 10 seconds
2. Range from: 1 hour up to 99 hours 59 minutes, in steps of 1 minutes
3. Range: Continuous Run "cont", can be interrupted by the key, "stop" (10).
-The running time can be pre-selected, with the lid opened or closed.
-To activate the setting of the running time, press the key "time" (6).
-In the display, "time" (A-3) flashes the indication: "m : s" or "h : m", depending on the
previous setting.
To set the desired value, use the adjustable knob (1). After exceeding 59 min 50 sec, the indication
changes automatically to, "h : m". After exceeding 99 hours 59 min, the word "cont" appears in the
display, "time" (A-3).
The continuous run can only be interrupted by pressing the key, "stop" (10). The time counts down,
as soon as the set speed is reached.
For all number marked text, please refer to, Figure 14.
Figure 14
2.4.3 Pre-Selection of Brake Intensity and Acceleration
Selecting the key, "accel/decel" (5), this function is activated.
By pressing the key once, the word "accel" (M7) flashes, in the display "accel/decel" (A-2). The
desired acceleration can be pre-selected, with the adjustable knob (1). The value 0 is equivalent to
the lowest acceleration and the value 9 is equivalent to the highest acceleration.
By pressing the key "accel/decel" (5) twice, in the display "accel/decel" (A-2), indicates the word
"decel" (M8). Now the desired brake intensity can be pre-selected, with the adjustable knob (1). The
value 9 is equivalent to the shortest possible brake time and the value 0 to longest possible brake
time.
See Table 5: "Acceleration and Deceleration Times", (See APPENDIX V). This table shows the
acceleration and deceleration times, for the acceleration and deceleration stages 0 to 9, for each
rotor.

OPERATION
16 Z206A_V1.18_eng © Hermle Labortechnik GmbH
Figure 15
2.5 Starting and Stopping the Centrifuge
2.5.1 Starting the Centrifuge
After closing the lid, start the centrifuge with the key, "start" (9). With the key "start" (9), runs can be
started, with manually pre-selected parameters. When the respective pre-selected running time has
ended, the centrifuge will stop automatically or the run can be interrupted, with the “stop” (10) key.
For all number marked text, please refer to Figure 16.
Figure 16