Hermle Z 206 A User manual

Instruction Manual
For
Compact Centrifuge
Z 206 A
Page 1 of 24 INSTRUCTION MANUAL Z 206 A www.bioecoms.com

CONTENTS
1PRODUCT DESCRIPTION
1.1 Usage in accordance with safety standards
1.1.1 General Information
1.1.1.1 Hazards and precautions
1.1.1.2 Brief description
1.1.1.3 Safety Standards
1.1.1.4 Extent of supply
1.1.1.5 Warranty
1.2 Installation
1.2.1 Installation of the centrifuge
1.2.1.1 Unpacking the centrifuge
1.2.1.2 Space requirements
1.2.1.3 Installation
1.3 Technical Data
1.4 Conformity Declaration
1.5 Basic Setup and the “operating data” mode
1.5.1 Access to the “basic adjustments” mode
1.5.2 Call up of the “operating data” mode
2OPERATION
2.1 Installation of rotors
2.1.1 Mounting and loading angle rotors
2.1.2 Mounting and loading swing out rotors
2.1.3 Overloading of rotors
2.1.4 Removing the rotors
2.2 Operation
2.2.1 Power switch
2.2.2 Lid release
2.2.3 Lid lock
2.2.4 Pre-selection of speed/RCF-value
2.2.5 Pre-selection of running time
2.2.6 Pre-selection of brake intensity and acceleration
2.2.7 Starting the centrifuge
2.2.8 “Stop“ key
2.3 Safety features
2.3.1 Imbalance detection
3MAINTENANCE
3.1 Service and Maintenance
3.1.1 Maintenance and cleaning
3.1.2 Glass breakage
3.1.3 Disinfection of Alu-rotors
3.1.4 Disinfection of PP-rotors
4TROUBLE SHOOTING
4.1 Error messages: Cause / Solution
4.2 Survey of possible error messages and their solutions
4.2.1 Lid release during power failure
4.2.2 Discription of the error message system
4.2.3 Error messages
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www.bioecoms.com 2004/01
1 PRODUCT DESCRIPTION
1.1 Usage in accordance with safety standards
1.1.1 General information
This unit is a medicine product according to the IVD guide line 98/79/EG.
1.1.1.1 Hazards and precautions
Before setting the centrifuge into operation, please read this instruction manual carefully!
For your personal safety, please review the following precautions:
•The HERMLE Z 206 A is not explosion-proof and must therefore not be operated in explosion-
endangered areas or locations. During centrifugation, it is prohibited to stay within the safety zone
of 30 cm around the centrifuge or deposit hazardous substances within this area.
•Centrifugation of flammable, explosive and radioactive substances, which
chemically react with high energy, is strictly prohibited!
•Never spin toxic or pathogenic material without adequate safety precautions, i.e. centrifugation of
buckets / tubes without or with defective hermetic sealings is strictly prohibited.
The user is obligated to perform appropriate disinfection procedures in the case that dangerous
substances have contaminated the centrifuge and / or the accessories. When centrifuging
infectious substances, always pay attention to the General Laboratory Precautions. If necessary,
contact your safety officer.
•It is prohibited to run the centrifuge with rotors that are not listed for this unit.
•Under no circumstances open the lid of the centrifuge while the rotor is still running or rotating with
a speed of > 2 m/s.
Following rules must strictly be adhered to:
•Do not operate the centrifuge in the case that it is not installed correctly.
•Do not operate the centrifuge when dismounted (e.g. without housing).
•Do not run the centrifuge when mechanical or electrical components have been tampered
with by unauthorized personnel.
•Do not use accessories such as rotors and buckets, which are not exclusively approved by
HERMLE Labortechnik GmbH, except commercially available centrifuge tubes made of
glass or plastic.
•Do not spin extremely corrosive substances, as they may cause material damages and
impair mechanical resistance.
•Do not operate the centrifuge with rotors or buckets, which show any signs of corrosion or
mechanical damage.
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1 PRODUCT DESCRIPTION
The manufacturer is responsible for safety and reliability of the centrifuge when:
•The unit is operated in accordance with this instruction manual.
•Modifications, repairs or other adjustments are performed by HERMLE-authorized personnel and
the electrical installation of the related location corresponds to the IEC-regulations.
1.1.1.2 Brief description
Model Z 206 A is a compact small centrifuge capable of running swing out and angle rotors. All
relevant run parameters can easily be set with the display keys and be pre-selected with the main
adjustment knob. All pre-selected and actual values are permanently displayed on large LCD display.
The lid is latched and released with an electronic lid lock.
The centrifuge has a powerful, maintenance-free motor with a low noise level.
1.1.1.3 Safety standards
The centrifuge corresponds with the General Requirements for Medical Units Regulations
(MedGV) (group 3).
Following standards have been considered for the production of our centrifuges:
•Accident Prevention Regulation for electrical units and installations UVV VBG 4
•Accident Prevention Regulation for centrifuges as per BGR 500; Chapter 2.11; Part 3
•DIN 58970 part 1, 2 and 4 for centrifuges and tubes
•Electrical Interference Suppression according to interference degree B as per VDE 0871
•Electrical Safety as per IEC 1010-1 and IEC 1010-2-D
•European Standard PR EN 61 010-1 and PR EN 61 010-2-2
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1 PRODUCT DESCRIPTION
1.1.1.4 Included Accessories
Following parts are supplied as accessories with each centrifuge:
• 1 Instruction manual
1.1.1.5 Warranty
The centrifuge has been subjected to thorough testing and quality control procedures.
In the unlikely case of any manufacturing faults occurring, the centrifuge and rotors are covered
by warranty for a period of two year from date of delivery.
This warranty becomes invalid in case of mishandling, negligence, and further in case of
usage of inappropriate spare parts and / or accessories, as well as any unauthorized
modification of the unit.
Technical modification rights are reserved by the manufacturer in respect to
technical improvement.
1.2 Installation
1.2.1 Installation of the centrifuge
1.2.1.1 Unpacking the centrifuge
Model Z 206 A is supplied in a carton.
Remove the strap retainer, open the carton and remove the centrifuge. The
instruction manual should always be kept with the centrifuge.
1.2.1.2 Space requirements
The centrifuge should be installed on an even and solid surface, if possible on a
laboratory cabinet / table or some other solid vibration free surface.
In order to enable a safe and smooth operation, level the centrifuge with a levelling device.
The centrifuge must be placed in a way, that there is a minimum space of 30 cm on each side of the
unit in order to ensure the necessary heat dissipation.
Do not place the centrifuge next to a window or a heater, where it could be disposed to excessive
heat, as the performance of the unit is based on an ambient temperature of 23°C.
Safety regulations require that the safety area of 30 cm around the unit is marked in order to indicate
that neither hazardous substances nor persons should be within this area during centrifugation.
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1 PRODUCT DESCRIPTION
1.2.1.3 Installation
Follow these steps:
•Check whether power supply corresponds with the one named on the manufacturer's rating
label which is mounted on the rear panel.
•The line voltage circuit breaker is max. 10 A (type K) slow release for commonly used instruments.
•In case of emergency, there must be an emergency switch off installed outside the room in order to
disconnect the power supply of the unit.
(• Remove the transport spacer blocks from the motor shaft (see chapter 2.2.2).)
The outlet for the power cord must be easy to reach and to disconnect!
1.3 Technical Data
Manufacturer
HERMLE Labortechnik GmbH
Type / Model
Z 206 A
Dimensions
28 cm
Width
Depth
37 cm
Height
26 cm
Weight
15 kg
Noise level (max.)
60 +2.0 dB (A)
Max. speed
6000 rpm
Max. volume
6 x 50 ml
Max. RCF
4185 x g
Admissible density
1.2 kg/dm3
Admissible kinetic energy
1694 Nm
Electrical connection AC
230 V / 50 Hz 1 ph
120 V / 60 Hz 1 ph
Current
0.55 A
1.1 A
Connected load
100 Watt
100 Watt
Interference suppression
VDE 0871, Funkentstörgrad B
Test obligations
To be filled in by purchaser:
Inventory-No.:
Check-No.:
Location:
Maintenance contract:
Your service department
Labnet International
PO Box 841, Woodbridge, NJ
Ph: 732-417-0700
Your agent
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1 PRODUCT DESCRIPTION
1.4 Conformity declaration
The CE-mark is always set by the manufacturer. Precondition is the issue of a declaration of conformity
according to Standard DIN EN 45014 in which the manufacturer declares the conformity of the product with all
relevant EU directions.
We, the company
Hermle Labortechnik GmbH
Siemensstrasse 25
78564 Wehingen
declare in mere responsibility that our product
Centrifuges
of models
Z 100 M; Z 160 M
Z 206 A
Z 233 M-2; Z 233 MK-2
Z 300; Z 300 K; SIEVA-2
Z 323; Z 323 K; Z 366; Z 36 HK; Z 383; Z 383 K
Z 400; Z 400 K; Z 513; Z 513 K
SETA Oil test centrifuge
as from month/year of construction (01 / 05)
to which this declaration refers to, have been manufactured according to the following standards or according
to normative documents.
DIN EN 61 010-1; DIN EN 61 010-2-020;
EN 50501/B;
EN 61000-6-1; EN 61000-3-2; EN 61000-3-3;
EN 292-2; EN 292-2/A1; EN 61326;
73/23/EWG; 98/37/EG; 98/79/EG
Wehingen/Germany, 1st January 2007
Harald Hermle
President
Page 7 of 24
INSTRUCTION MANUAL Z 206 A
www.bioecoms.com

1 PRODUCT DESCRIPTION
1.5 Initial Setup
Before the first operation and after each rotor change, you have to select the appropriate rotor code.
You can find each rotor type in the printed order number on the rotor.
Example:
Angle rotor
220.96
V01
≙ Rotor type “96“
Swing out rotor
220.68
V04
≙ Rotor type “68“
Switch on the unit and open the lid. Now press simultaneously the keys “lid “ (1) and “stop” (2). In the
display “rpm/rcf” then appears the selected rotor type, ex. “96”. With the adjustment knob (7) you can
select the desired rotor code. (See photo 1) To set the data in the unit please wait until the indication
in the display “rpm/rcf” disappears.
Now all important rotor parameters for the centrifuge are stored.
Photo 1
1
2
7
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1 PRODUCT DESCRIPTION
1.5.1 Access to the “Operating Data“ Mode
In this mode you can check the following points:
1. Number of starts
2. Operating hours of the centrifuge
3. Software-version
4. Error list
5. Operation of imbalance sensor
6. Operation of keyboard
7. Display test
If the centrifuge is still turned off, press simultaneously the keys “time“ (4) and “lid“ (1) and turn on
the main switch of the centrifuge. Now release both keys again. As a result a display test is
executed for approx. 5 seconds. All possible indications will appear at the same time (see photo 2 ).
Photo 2
4
1
7
ATTENTION:
Please notice that you must enter the program as described under point 1.5.1 to change the
adjustments of the points 1.5.2. After you have stored the settings you can return to the
standard program mode again by switching off and the switching on the centrifuge.
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1 PRODUCT DESCRIPTION
1.5.2 Call up of operating data
In the mode “Initial Setup“ you can call up the operating data of the centrifuge.
Please proceed as described under point 1.5.1 to enter this program mode.
Press the key “accel/decel“ (5). In the display “accel/decel“ flashes the word
“service“. By adjusting the adjusting knob (7) the information below can be called up:
A= previous starts of the centrifuge (see photo 3a)
H= previous operating hours
S= software version
E= list of previous error messages
F
Uonly for service purpose
P
C
The list of the last 99 error messages can be looked over by pressing the key “rpm/rcf“ (6) and scrolling
through it by the adjusting knob (7). The respective error codes appear in the display “rpm/rcf“
(see photo 3b). Please look up in chapter 4.2.3 of this instuction manual for the detailed description.
The first two numbers (X) indicate the quantity of errors ongoing from 00 to 99, the last two numbers
(Y) indicate the error code (ex. 02 = imbalance sensor is defective).
XY
6
5
7
Photo 3a
Photo 3b
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2 OPERATION
2.1 Installation of rotors
2.1.1 Mounting and loading angle rotors
Clean the drive shaft with a clean, grease-free piece of
cloth. Place the rotor onto the drive shaft. (see photo 4)
Be sure that the rotor is fully installed on the motor shaft.
Photo 4
Photo 5
ATTENTION:
For safety always ensure that rotor fixing screw is tightened before each run. (see photo 5)
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2 OPERATION
Hold the rotor with one hand and secure the rotor to the shaft by turning the fixing screw
(1) clockwise. (see photo 6).
1
photo 6
ATTENTION:
For safety always ensure that rotor fixing screw is tightened before each run. (see photo 6)
It is allowed to operate a 12-place-rotor with 2 or 4 loaded tubes only.
But the loaded borings must be opposite each other.
Photo 7: incorrect Photo 8: correct
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2 OPERATION
2.1.2 Mounting and loading swing out rotors
Clean the drive shaft with a clean and grease-free cloth. Place the rotor to the motor shaft. Be sure
that the rotor is fully installed onto the motor shaft.
Hold the rotor with one hand and secure the rotor to the shaft by turning the rotor screw (1) clockwise.
The installation of the buckets and the adapters must be done appropriately photo 10.
In principle swing out rotors may not be taken in operation untill all buckets or racks are put
into the rotor.
The bolts at the rotor must be greased with silicone grease.
The sample tubes have to be filled evenly by eye and put into the drillings or tube racks. The
weight difference of the loaded buckets should not exceed approx. 1.0 g.
It is allowed to operate e.g. a 6-place-rotor with 2 loaded buckets only. But the loaded buckets must
be opposite to each other. Make sure that the unloaded buckets also be put inside the rotor (see
photo 9 and 10).
ATTENTION:
Always fill all rotor positions with rotor buckets.
Photo 9: incorrect Photo 10: right
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2 OPERATION
2.1.3 Overloading of rotors
The maximum load permitted for a rotor, which is determined by the manufacturer, as well as
the maximum speed allowed for this rotor (see label on rotor), must not be exceeded.
The liquids the rotors are loaded with, should have an average homogeneous density of 1,2 g/ml or
less when the rotor is running at maximum speed.
In order to spin liquids with a higher density, the speed has to be reduced according to the
following formula:
Reduced speed nred
1,2
=
x max. speed (nmax) of the rotor
higher density
Example:
1,2
nred
=
x 4.000 = 3.360 rpm
1,7
In case of any questions, please contact the manufacturer.
2.1.4 Removing the rotor
Untighten the rotor fixing screw and lift the rotor vertically out of the centrifuge.
ATTENTION:
Do not operate the centrifuge with rotors or buckets which show any signs of corrosion
or mechanical damage.
Do not operate with extremely corrosive substances which could damage the rotor and buckets.
2.2 Operation
2.2.1 Power switch
The power switch is located on the bottom, left side of the unit.
Photo 11
Attention:
After turning on the power switch you must open the lid of the unit first, prior starting
the centrifuge.
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2 OPERATION
2.2.2 Lid release
Once the centrifuge lid is closed the display shows “rpm/rcf“ and “close“ (8). At the same time the pre-
selected rotor type is indicated, ex.. “nr 96” (11). During the run you can call up the rotor type at any
time by pressing the key “lid” (1). By pressing the key “lid“ (1) you can release the lid of centrifuge. As
soon as the electronic lid is completely released, it appears the word “open“ (9). Now you can open the
lid of the centrifuge. (see photo 12)
8
10
11
1
9
Photo 12
2.2.3 Lid lock
Attention: Before closing the lid please ensure that the rotor is tight.
As a sign that the centrifuge is ready for operation the display reads ‘rpm/rcf“ the word “close“ (8).
Simultaneously it appears in that display the word “rotor“ (10), as well as the code number of the
rotor, which is in the centrifuge ex. “nr 96“ (11).
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2 OPERATION
2.2.4 Pre-selection of speed / RCF-value
By pressing the key “rpm/rcf“ (6) the pre-selection of the speed is activated. By pressing the key
once the word “rpm“ (12) flashes.
By pressing the key once again the pre-selection of the centrifugal forces may be chosen. Flashing
the word “rcf“ (13).
You can set the desired values with the adjusting knob (7). In the display (14) the regulated value
is shown permanently, before, during and after the run.
The speed is adjustable between 200 rpm and maximum revolution of the centrifuge resp. the
maximum permissible revolution of the pre-selected rotor.
It is the same with the pre-selection of the RCF-value. The setting range is between 20 xg and
the maximum permissible centrifugal force of the rotor.
The maximum speed of the Z 206 A is 6000 rpm resp. 4180 xg.
13
12 14 14
67
Photo 13
Maximum speed of the rotors available for the Z 206 A
Rotor-Number
Max.
RCF
Revolution
Value
220.96 V01
6000 rpm
4180 xg
220.97 V01
6000 rpm
3820 xg
220.95 V06
6000 rpm
2930 xg
220.68 V04
3500 rpm
1450 xg
Attention:
Please also check the maximum permissible speed of your test tubes.
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2 OPERATION
2.2.5 Pre-selection of running time
The running time can be pre-selected in three different ranges from 10 seconds up to 99 hours
59 minutes.
1. Range from 10 seconds up to 59 minutes 50 seconds in steps of 10 seconds
2. Range from 1 hour up to 99 hours 59 minutes in steps of 1 minutes
3. Range continuous run “cont”, which can be interupted by the key “stop” (2).
The running time can be pre- selected with the lid opened or closed.
To activate the setting of the running time press the key “time“ (4).
In the display “time“ flashes the indication “m : s“ or “h : m“ (15), depending on the previous setting. To
set the desired value use the adjusting knob (7). After exceeding of 59 min 50 sec the indication (15)
changes automatically to “h : m“. After exceeding of 99 hours 59 min the word “cont” appears in the
display “time”. That continuous run can only be interupted by pressing the key “stop” (2).
The display shows always the remaining running time.
15
15
2
4
Photo 14
7
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2 OPERATION
2.2.6 Pre-selection of brake intensity and acceleration
By pressing the key “accel/decel“ (5) this function is activated.
By pressing the key once the word “accel“ (16) flashes in the display “acc/dec“. The desired
acceleration can be pre-selected by the adjusting knob (7). The value 0 is equivalent to the lowest
and the value 9 to the highest acceleration.
By pressing the key “accel/decel“ (5) twice, in the display “acc/dec“ indicates the word “decel“ (17).
Now the desired brake intensity can be pre-selected by the adjusting knob (7). The value 9 is
equivalent to the shortest and the value 0 to longest possible brake time.
57
16 17
Photo 15
Acceleration and deceleration times Z 206 A(120 V / 230 V) in seconds
Acceleration values
Deceleration values
Rotor-Number
Level 0
Level 9
Level 0
Level 9
220.96 V01
70
40
150
35
220.97 V01
80
35
150
35
220.95 V06
60
11
30
10
220.68 V04
35
8
25
7
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2 OPERATION
2.2.7 Starting the centrifuge
After closing the lid you can start the centrifuge with the key “start“ (3).
By pressing the key “start“ (3) you can start runs with manually pre-selected parameters.
When the respective pre-selected running time has ended then the centrifuge will stop automatically
or you can interupt the run in the mode “cont” with the key “stop” (2).
2
3
Photo 16
2.2.8 The „STOP“ key
By the “stop“ key (2) you can interrupt the run at any time. After pressing the key the
centrifuge decelerates with the respective pre-selected intensity down to stand still.
2
Photo 17
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2 OPERATION
2.3 Safety features
2.3.1 Imbalance detection
In case of the rotor not being equally loaded, the drive will turn off during acceleration. The
rotor decelerates to stand still.
When in the display “time” the word “error” together with the number “01” (18) appear, the
weight difference of the samples is too huge. Weigh out the samples exactly.
Load the rotor as described in chapter 2.1.1.
When in the display “time” the word “error” together with the number “02“ (18) appear, there could
be following reasons:
• The imbalance switch is defective.
18
Photo 18
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