Hermle Z 216 M User manual

© Hermle Labortechnik GmbH Z216M_V2.21_eng
HERMLE Z 216 M
User Manual

CONTENTS
© Hermle Labortechnik GmbH Z216M_V2.21_eng I
1. PRODUCT DESCRIPTION..................................................................................... 1
1.1 Safety Instructions ............................................................................................................................................ 1
1.2 Intended Purpose .............................................................................................................................................. 1
1.3 Brief Description................................................................................................................................................ 1
1.4 Delivery Package ............................................................................................................................................... 1
1.5 Installation of the Centrifuge ............................................................................................................................ 2
1.5.1 Unpacking the Centrifuge................................................................................................................................ 2
1.5.2 Space Requirements....................................................................................................................................... 2
1.5.3 Installation ....................................................................................................................................................... 2
1.6 Signs and Indications of the Centrifuge .......................................................................................................... 3
1.6.1 Product Nameplate(Example) ......................................................................................................................... 3
1.6.2 Warning and Information Signs....................................................................................................................... 4
1.7 Operating and Display Elements...................................................................................................................... 5
1.7.1 LCD-Display .................................................................................................................................................... 6
1.8 Danger, Precautions and Warranty.................................................................................................................. 7
1.8.1 Following Rules Must Strictly be Adhered To:................................................................................................. 7
1.8.2 Warranty.......................................................................................................................................................... 8
1.9 Basic Adjustments ............................................................................................................................................ 9
1.9.1 Adjustment of the Rotor Type.......................................................................................................................... 9
1.9.2 Access to Mode: "Operating Data"................................................................................................................ 10
1.9.3 Signal Turn On / Off (optional)...................................................................................................................... 11
1.9.4 Volume Pre-Selection of Sound Signal (optional).......................................................................................... 11
1.9.5 Song Selection- End of Run (optional) .......................................................................................................... 12
1.9.6 Keyboard Sound Turn On / Off (optional)...................................................................................................... 12
1.9.7 Retrieving Operating Data (operated by skilled or service engineer only!).................................................... 13
2. OPERATION ........................................................................................................ 14
2.1 Mounting and Loading the Angle Rotor ........................................................................................................ 14
2.1.1 Installation of Rotors...................................................................................................................................... 14
2.1.2 Loading Angle Rotors.................................................................................................................................... 14
2.1.3 Loading and Overloading of Rotors............................................................................................................... 15
2.1.4 Removing the Rotor ...................................................................................................................................... 15
2.2 Lid ..................................................................................................................................................................... 16
2.2.1 Lid Release ................................................................................................................................................... 16
2.2.2 Lid Lock......................................................................................................................................................... 16
2.3 Pre-Selection.................................................................................................................................................... 17
2.3.1 Pre-Selection of Speed / RCF-Value............................................................................................................. 17
2.3.2 Pre-Selection of Running Time...................................................................................................................... 17
2.3.3 Pre-Selection of Brake Intensity and Acceleration........................................................................................ 18
2.4 Radius Correction............................................................................................................................................ 19
2.5 Program............................................................................................................................................................ 19
2.5.1 Storage of Programs ..................................................................................................................................... 19
2.5.2 Recall of Stored Programs ............................................................................................................................ 20
2.5.3 Leaving Program Mode................................................................................................................................. 21

CONTENTS
II Z216M_V2.21_eng © Hermle Labortechnik GmbH
2.6 Starting and Stopping the Centrifuge............................................................................................................ 21
2.6.1 Starting the Centrifuge................................................................................................................................... 21
2.6.2 The "STOP" Key............................................................................................................................................ 21
2.7 Imbalance Detection........................................................................................................................................ 22
2.8 Timer Function................................................................................................................................................. 22
3. MAINTENANCE ................................................................................................... 24
3.1 Maintenance and Cleaning.............................................................................................................................. 24
3.1.1 General Care:................................................................................................................................................ 24
3.1.2 Cleaning and Disinfecting of the Unit ............................................................................................................ 25
3.1.3 Cleaning and Disinfecting of the Rotor.......................................................................................................... 25
3.1.4 Disinfection of Aluminum Rotors ................................................................................................................... 26
3.1.5 Disinfection of PP-Rotors .............................................................................................................................. 26
3.1.6 Glass Breakage............................................................................................................................................. 26
3.2 Lifetime of Rotors, Round and Rectangular Buckets, Accessories............................................................ 26
4. TROUBLE SHOOTING......................................................................................... 27
4.1 Error Message: Problem / Solution................................................................................................................ 27
4.2 Survey of Possible Error Messages and Solutions ...................................................................................... 27
4.2.1 Lid Release during Power Failure (Emergency Lid Release)........................................................................ 27
4.2.2 Description of the Error Message System..................................................................................................... 28
4.2.3 Procedure while error 14, from software version 1.76................................................................................... 28
4.2.4 Procedure while error 38 –Lid motor is blocked ........................................................................................... 29
5. RECEIPT OF CENTRIFUGES TO REPAIR.......................................................... 30
6. TRANSPORT, STORAGE AND DISPOSAL ........................................................ 31
6.1 Transport.......................................................................................................................................................... 31
6.2 Storage ............................................................................................................................................................. 31
6.3 Disposal............................................................................................................................................................ 31
7. APPENDIX .............................................................................................................. I
EG - Conformity Declaration .................................................................................................................................... II
Table 1: Technical Data .......................................................................................................................................... III
Table 2: Permissible Net Weight.............................................................................................................................IV
Table 3: Max. Speed and RCF-Values for Permissible Rotors ...............................................................................IV
Table 4: Acceleration and Deceleration Times........................................................................................................IV
Table 5: Error Messages..........................................................................................................................................V
Table 6 Radius Correction ......................................................................................................................................VI
Table 7: Abbreviations ............................................................................................................................................VI
Redemption Form / Decontamination Certificate ...................................................................................................VII

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z216M_V2.21_eng 1
1. PRODUCT DESCRIPTION
1.1 Safety Instructions
This symbol indicates safety instructions and points of potential dangerous situations. Before using
the centrifuge for the first time, please read the operating manual.
Failure to follow these instructions can result in personal injury and/or property damage .
Intended use includes: the observation of all instructions, in the instruction manual, and
administering inspection and maintenance.
1.2 Intended Purpose
This Hermle centrifuge was designed only for the separation of materials or mixtures with different
densities, specifically for the preparation and processing of samples, from the human body, in
context of an in-vitro-diagnostic use, to allow the use of in-vitro-diagnostic in accordance to its'
intended purpose. The designated device and its' accessories listed, in the technical documentation,
are in compliance with Directive 98/79/EC for In-Vitro-Diagnostic Medical Devices.
Hermle Centrifuges are intended exclusively for indoor use and for the use of qualified personnel.
Only Hermle original rotors and accessories should be used. Any other use or intended use is strictly
prohibited. For any resulting damage, the company, Hermle Labortechnik, is not liable.
1.3 Brief Description
The unit type Z 216 M, is a microlitre centrifuge, which we offer in two voltage variations 230V or
120V.
The centrifuge can be used with angle rotors.
All parameters are accessible via buttons and selected with the central adjuster. All pre-selected and
current values will be shown permanently on the LCD-display.
The centrifuge is powered by a maintenance-free induction motor.
Detailed technical data are in, Table 1: "Technical Data", (see APPENDIX S.III).
1.4 Delivery Package
•1 Centrifuge Z 216 M
•1 Operating Manual Z 216 M
•1 Rotor key
*Rotor(s) / Accessories will be packaged separately.

PRODUCT DESCRIPTION
2Z216M_V2.21_eng © Hermle Labortechnik GmbH
1.5 Installation of the Centrifuge
1.5.1 Unpacking the Centrifuge
Model Z 216 M is supplied in a carton.
Remove the strap retainer, open the carton, and remove the centrifuge. The instruction manual must
be kept with the centrifuge, at all times!
1.5.2 Space Requirements
The centrifuge should be installed on an even, solid surface, if possible on a laboratory cabinet /
table or some other solid vibration free surface.
During centrifugation, the centrifuge must be placed in a way, that there is a minimum space of
30 cm/11.81in on each side of the unit, according to EN 61010-2-020 standards.
Do not place the centrifuge next to a window or a heater where it could be disposed to excessive
heat, as the performance of the unit is based on an ambient temperature of 23°C/73.4°F.
1.5.3 Installation
Follow These Steps:
•Check whether the power supply corresponds with the one specified on the manufacturer's rating
label, mounted on the rear panel.
•The power connection for the centrifuge requires a separate one-site protection, with 16 A (Type K)
•In case of emergency, there must be an emergency switch off installed outside of the room, in order
to disconnect the power supply from the unit.
•Connect the centrifuge, with the mains.
(The socket for the power cord must be easy to reach, respectively easy to disconnect).
Switch on, by using the mains power switch (I).
Open the lid, by using the button LID.
• Remove the transport securing device of the motor.

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z216M_V2.21_eng 3
1.6 Signs and Indications of the Centrifuge
1.6.1 Product Nameplate(Example)
Company Address:
Hermle Labortechnik GmbH, Siemensstr. 25, D-78564 Wehingen
In-Vitro-Diagnostic Medical Device
TYPE:
Type Designation of the Product
REF:
Order No. of the Product
SN:
Serial No. of the Product
Manufacturer
Date of Manufacture
MAX. Drehzahl:
Max. Speed Allowed of the Unit
KIN. EN.:
Max. Kinetic Energy with Corresponding Rotor
U/I/f:
Allowable Voltage / Max. Current / Frequency
P:
Electrical Input Power
Operating Manual Indication
Labeling, Standards and Guidelines
Instructions for Disposal
RoHS Compliance

PRODUCT DESCRIPTION
4Z216M_V2.21_eng © Hermle Labortechnik GmbH
1.6.2 Warning and Information Signs
Four carriers must be used at all times on four places swing out
rotors or damage will occur to the centrifuge. Such damage will not
be covered under the product warranty
Attention! Check the fastening of the rotor nut before each run
Take off mains plug before opening the housing or the emergency
release
Power Input
Direction of Rotation –clockwise rotation for the rotor drive
Rotation Direction Emergency Release
Biohazard Warning

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z216M_V2.21_eng 5
1.7 Operating and Display Elements
1central adjuster Run Parameters
20-I Power Switch
3LCD Control Panel Display
4rpm/rcf Speed/ g-force
5 accel/decel Acceleration / Deceleration Intensity
6time Centrifugation Time
7 lid Lid Release
8quick Short Running
9start Start Centrifugation
10 stop Stop Centrifugation
11 prog Retrieving Stored Programs
12 store Program Store
11 12 1
8
10
3
4
5
6
7
9
2

PRODUCT DESCRIPTION
6Z216M_V2.21_eng © Hermle Labortechnik GmbH
1.7.1 LCD-Display
The following picture shows the individual elements of the LCD-display.
Figure 1
Display Fields:
A-1 Display Field –"rpm/rcf"
A-2 Display Field –"acc/dec"
A-3 Display Field –"time"
Messages/Logos of the Display Fields:
M1 "close" M8 "decel"
M2 "open" M9 "radius"
M3 "rotor" M10 "program"
M4 Rotor-No. M11 "error"
M5 "rpm" M12 "service"
M6 "rcf" M13 h m s
M7 "accel"

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z216M_V2.21_eng 7
1.8 Danger, Precautions and Warranty
This device may only be operated by a trained professional. Carefully, read the
operating manual and be familiar with the functions of the device.
To protect people and the environment, the following precautions must be taken:
•During centrifugation, the presence of people and the arrangement of hazardous materials is
strictly prohibited, within 30 cm/12 in around the centrifuge, according to the regulations of
EN 61010-2-020.
•The HERMLE Z 216 M is a non "explosion-proof" and must not be operated in explosion-
endangered areas or locations. Centrifugation of flammable, explosive, radioactive, or such
substances, which chemically react with high energy, is strictly prohibited. If used in such
environment, this is at the users own expense.
•Never spin toxic or pathogenic material without adequate safety precautions, i.e.
centrifugation of buckets / tubes with or without defective hermetic sealing, is strictly
prohibited. The user is obliged to perform appropriate disinfection procedures, in case
dangerous substances have contaminated the centrifuge and/or its' accessories. When
centrifuging infectious substances, always pay attention to the General Laboratory
Precautions. If necessary, contact your safety officer!
•It is prohibited to run the centrifuge, with rotors not manufactured for this unit.
•Under no circumstances open the lid of the centrifuge, while the rotor is still running or
rotating with a speed of > 2m/s.
1.8.1 Following Rules Must Strictly be Adhered To:
•Do not operate the centrifuge if not installed correctly.
•Do not operate the centrifuge when dismounted (e.g. without housing).
•Do not run the centrifuge, if mechanical or electrical assembly groups have been tampered
with, by unauthorized personnel.
•Do not use accessories such as rotors and buckets, that are not approved by HERMLE
Labortechnik GmbH, except commercially available centrifuge tubes, made of glass or
plastic.
•Do not spin extremely corrosive substances, as they may cause material damages and impair
mechanical resistance.
•Do not operate the centrifuge with rotors or buckets, that show any signs of corrosion or
mechanical damage.
The manufacturer is responsible for safety and reliability, of the centrifuge, only if:
•The unit is operated in accordance to this instruction manual.
•Modifications, repairs or other adjustments are performed by HERMLE-authorized personnel
and the electrical installation of the related location corresponds to the IEC-regulations.

PRODUCT DESCRIPTION
8Z216M_V2.21_eng © Hermle Labortechnik GmbH
1.8.2 Warranty
The centrifuge has been subjected to thorough testing and quality control. In the unlikely case of any
manufacturing faults occurring, the centrifuge and rotors are covered by warranty, for a period of two
years, from date of delivery. This warranty becomes invalid in any case of mishandling, damage
and/or negligence and further in any case of usage of inappropriate spare parts and / or accessories
or unauthorized modification of the unit.
Technical modification rights are reserved, by the manufacturer, in regards to technical
improvement!

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z216M_V2.21_eng 9
1.9 Basic Adjustments
1.9.1 Adjustment of the Rotor Type
Before the first operation resp., after each rotor change, select the respective rotor type. Each rotor
type is found, on the printed order number, on the rotor.
Example:
Angle Rotor 220.87 V11 ≙rotortype "87"
Switch on the unit and open the lid. Now simultaneously press the keys, "lid " (7) and "stop"(10). In
the display, "rpm/rcf" (A-1), appears the previously selected rotor type, i. e. “87”. Now the buttons
can be released.
With the potentiometer (1), choose the previously used rotor, (see Figure 2). Select the data in the
unit, please press the “start” (9) key. Inside the display, (A-1), the word “store” appears, as the
confirmation.
Now all important rotor parameters for the centrifuge are stored.
Figure 2
Attention: Set rotor must always match with the used rotor, otherwise the unit could be
damaged.
The rotor type can be checked during the run, by pressing the key "lid" (7).
Before operating the centrifuge, please notice the following optional basic setting changes:
-Acoustic Signal Turn On / Off
-Keyboard Sound Turn On / Off
-Volume Pre-Selection of Sound Signal
-Song Selection for "end of run"

PRODUCT DESCRIPTION
10 Z216M_V2.21_eng © Hermle Labortechnik GmbH
1.9.2 Access to Mode: "Operating Data"
If the centrifuge is still turned off, press the keys, "time" (6) and "lid" (7), simultaneously in order to
turn on the main switch of the centrifuge. Now release both keys. As a result, a display test is
administered for approx. 5 seconds. All possible indications will appear at the same time, (see
Figure 3).
Figure 3
ATTENTION:
-Please notice that you must enter the program as described, under point 1.9.2,to
change the adjustments of the points 1.8.3 - 1.8.7. After the settings have been
stored by user, the normal program mode can be changed back again by
switching off the centrifuge, for a short period.
-All changed settings must be confirmed by the key, "start" (9). A confirmation
screen will appear with the word, "store", in the display "rpm/rcf"(A-1) - Only then
the pre-selections are valid, (see Figure 4)!
Figure 4
1
7

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z216M_V2.21_eng 11
1.9.3 Signal Turn On / Off (optional)
Proceed as illustrated, under point 1.8.2, to enter this program mode, press the key; "accel/decel"
(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter, "L" with the
adjustable knob (1). As a result, appearing in the display "rpm/rcf" (4), are the words, "On Sound".
By pressing the key, "rpm/rcf" (4), the word "On" flashes, and the sound can be switched off with the
adjustable knob (1), (see Figure 5).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
Figure 5
1.9.4 Volume Pre-Selection of Sound Signal (optional)
Proceed as illustrated, under point 1.8.2, to enter this program mode, press the key "accel/decel" (5).
In the display, "accel/decel" (A-2), flashes the word, "service". Select the letter, "U" with the
adjustable knob (1). As a result, appearing in the display, "rpm/rcf" (A-1), are the words, "Vol=0-
9/Sound". By pressing the key, "rpm/rcf" (4), the desired volume can be adjusted between 0 (low)
and 9 (loud), with the adjustable knob (1), (see Figure 6).
After the settings have been stored by user (see 1.8.2), the normal program mode can be changed
back again by switching off the centrifuge, for a short period.
Figure 6
9
8
1
7
2
3

PRODUCT DESCRIPTION
12 Z216M_V2.21_eng © Hermle Labortechnik GmbH
1.9.5 Song Selection- End of Run (optional)
Proceed as described under point 1.8.2 to enter this program mode and then press the key
„accel/decel“ (5). In the display „accel/decel“ (A-2) flashes the word „service“. Now select the letter
„G“. with the adjusting knob (1). As a result appears in the display „rpm/rcf“ (A-1) the word „
SonGo/Sound“. After pressing the key „rpm/rcf“ (4), you can select a song with the adjusting knob
(1). (see figure 7).
After you have stored the settings (see 1.8.2) you changed to the normal program mode again by
switch off the centrifuge for a short while.
Figure 7
1.9.6 Keyboard Sound Turn On / Off (optional)
Proceed as illustrated, under point 1.8.2, to enter this program mode, press the key, "accel/decel"
(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter "b" with the
adjustable knob (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word "ON/BEEP". After
pressing the key, "rpm/rcf" (4), turn the keyboard sound (On) or (Off), with the adjustable knob (1),
(see Figure 8).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
Figure 8

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z216M_V2.21_eng 13
1.9.7 Retrieving Operating Data (operated by skilled or service engineer only!)
In the function, "Basic Adjustments", retrieving the operating data of the centrifuge is an available
option. Please proceed as illustrated, under point 1.8.2, to enter this program mode, press the key,
"accel/decel" (5). In the display, "accel/decel" (A-2) flashes the word, "service".
With the adjustable knob (1), the following information can be retrieved:
A = Previous Starts of the Centrifuge
H = Previous Operating Hours
S = Software Version
r = Converter Software
E = List of Previous Error Message
h = Running Time of the Motor
The list of the last 99 error messages can be reviewed by pressing the key, "rpm/rcf" (4) and scroll
through, with the adjustable knob (1). The respective error codes appear in the display, "rpm/rcf" (A-
1). Please refer to: "Table 5: Error Messages", (see APPENDIX S. V).
Switch off the centrifuge, to return to the normal program mode.
Figure 9

OPERATION
14 Z216M_V2.21_eng © Hermle Labortechnik GmbH
2. OPERATION
2.1 Mounting and Loading the Angle Rotor
2.1.1 Installation of Rotors
Clean the drive shaft and the rotor shaft with a clean, grease-free piece of cloth. Place the rotor onto
the drive shaft, (see Figure 10). Please be sure that the rotor is fully installed onto the motor shaft.
Figure 10 Figure 11
Hold the rotor with one hand and secure the rotor to the shaft, by turning the fixing nut clockwise.
Tighten the fixing nut with the provided Allen key, (see Figure 11).
Figure 12
ATTENTION: For safety, always ensure that the rotor fixing screw is tightened before
each run, (see Figure 11)!
2.1.2 Loading Angle Rotors
Rotors must be loaded symmetrically and with equal weight, (see Figures 13 and 14). The adapter
may only be loaded with the appropriate vessels. The weight differences between the filled vessels
should be kept as low as possible. Therefore, we recommend to weigh with a balance. This reduces
the wear of drive and the acoustic operating noise.
Each rotor indicates what the maximum capacity is per hole. (It is allowed to operate e.g. a 12-place-
rotor with 2 or 4 loaded tubes only, but the loaded borings must be opposite of each other).

OPERATION
© Hermle Labortechnik GmbH Z216M_V2.21_eng 15
Figure 13: RIGHT Figure 14: WRONG
ATTENTION!
Do not operate the centrifuge with rotors or buckets that show any signs of corrosion
or mechanical damage.
Do not operate with extremely corrosive substances, which could damage the rotor
and buckets.
In case of any questions, please contact the manufacturer!
2.1.3 Loading and Overloading of Rotors
All approved rotors are listed with the maximum speed and maximum filling weight in,
Table 2: "Permissible Net Weight", (see APPENDIX P. IV).
The maximum load permitted for a rotor is determined by the manufacturer, as well as the maximum
speed allowed for this rotor (see label on rotor), must not be exceeded. The liquid the rotors are
loaded with, should have a max. homogeneous density of 1.2 g/ml or less when the rotor is running
at maximum speed.
In order to spin liquids with a higher density, the speed has to be reduced, according to the following
formula:
Reduced speed nred =√1,2
ℎ𝑖𝑔ℎ𝑒𝑟 𝑑𝑒𝑛𝑠𝑖𝑡𝑦 x max. speed (nmax) of the rotor
Example:
nred = √ 1,2
1,7 x 4.000 = 3.360 rpm
If In case of any questions, please contact the manufacturer!
2.1.4 Removing the Rotor
Untighten the rotor fixing nut, completely. Lift the rotor vertically out of the centrifuge, (see Figure 11).

OPERATION
16 Z216M_V2.21_eng © Hermle Labortechnik GmbH
2.2 Lid
2.2.1 Lid Release
After the run, properly close the lid of the centrifuge, appearing in the display, "rpm/rcf"(A-1) with the
word, "close" (M1). If there is a rotor in the centrifuge, the word, "rotor" (M3) appears, as well as the
code number of the specified rotor, which is in the centrifuge, for example "220.72" (M4). If there is
no rotor in the centrifuge, it flashes the word, "rotor" (M3) and an additional word, "no" (M4). ). By
pressing the key, "lid" (7), the lid of centrifuge can be released. As soon as the electromagnetic lid is
completely released, the word, "open" (M2) appears. The lid of the centrifuge is now able to be
opened.
For all number marked text, please refer to Figure 15.
Figure 15
During the run, you can retrieve the rotor type at any time, by pressing the key, “lid” (7).
2.2.2 Lid Lock
The lid must only be closed slightly. An electromagnetic lid lock closes the lid, the word "open" (M2)
will no longer be displayed.
As a sign that the centrifuge is ready for starting, appearing in the display, "rpm/rcf"(A-1), the word
"close" (M1). Simultaneously, the word "rotor" (M3) appears, as well as the code number of the
rotor, which is in the centrifuge, i. e. "nr 22x.xx“ (M4), along with all rotor specific data, for example:
max. speed, acceleration etc., are available.
For all number marked text, please refer to Figure 17.
ATTENTION: Don't grip your fingers between the lid and the device or the locking
mechanism, when closing the lid!