Hermle Z 366 K User manual

HERMLE Z 366 K
User Manual

CONTENTS
Z366K_V1.22_eng © Hermle Labortechnik GmbH
1. PRODUCT DESCRIPTION....................................................................................................................4
1.1 Safety Instructions .....................................................................................................................................4
1.2 Intended Purpose .......................................................................................................................................4
1.3 Brief Description.........................................................................................................................................4
1.4 Delivery Package ........................................................................................................................................4
1.5 Operating and Display Elements...............................................................................................................5
1.5.1 LCD-Display .............................................................................................................................................. 6
1.6 Signs and Indications of the Centrifuge...................................................................................................7
1.6.1 General...................................................................................................................................................... 7
1.6.2 Product Nameplate (Example) .................................................................................................................. 7
1.6.3 Warning and Information Signs ................................................................................................................. 8
1.6.4 Danger, Precautions and Warranty ........................................................................................................... 8
1.6.5 Following Rules Must Strictly be Adhered To:........................................................................................... 9
1.6.6 Warranty.................................................................................................................................................... 9
1.7 Installation of the Centrifuge ...................................................................................................................10
1.7.1 Unpacking the Centrifuge........................................................................................................................ 10
1.7.2 Space Requirements............................................................................................................................... 10
1.7.3 Installation ............................................................................................................................................... 10
1.7.4 Attention before first use!......................................................................................................................... 11
1.8 Basic Adjustments ...................................................................................................................................12
1.8.1 Access to Mode: "Operating Data".......................................................................................................... 12
1.8.2 Temperature Indication............................................................................................................................ 13
1.8.3 Sound Signal Turn On / Off ..................................................................................................................... 13
1.8.4 Volume Pre-Selection of Sound Signal ................................................................................................... 14
1.8.5 Song Selection- End of Run.................................................................................................................... 14
1.8.6 Keyboard Sound Turn On / Off................................................................................................................ 15
1.8.7 Retrieving Operating Data (operated by skilled or service engineer only!).............................................. 16
2. OPERATION........................................................................................................................................17
2.1 Mounting and Loading the Angle Rotor .................................................................................................17
2.1.1 Installation of Rotors................................................................................................................................ 17
2.1.2 Loading Angle Rotors.............................................................................................................................. 18
2.1.3 Loading Swing Out Rotors....................................................................................................................... 18
2.1.4 Loading and Overloading of Rotors......................................................................................................... 19
2.1.5 Removing the Rotor ................................................................................................................................ 19
2.2 Lid ..............................................................................................................................................................20
2.2.1 Lid Release ............................................................................................................................................. 20
2.2.2 Lid Lock................................................................................................................................................... 20
2.3 Pre-Selection.............................................................................................................................................21
2.3.1 Pre-Selection of Speed / RCF-Value....................................................................................................... 21
2.3.2 Pre-Selection of Running Time................................................................................................................ 22
2.3.3 Pre-Selection of Brake Intensity and Acceleration.................................................................................. 23
2.3.4 Pre-Selection of Temperature ................................................................................................................. 24
2.3.5 Pre-Cooling ............................................................................................................................................. 24

CONTENTS
© Hermle Labortechnik GmbH Z366K_V1.22_eng 2
2.4 Radius Correction.....................................................................................................................................25
2.5 Program.....................................................................................................................................................26
2.5.1 Storage of Programs ............................................................................................................................... 26
2.5.2 Recall of Stored Programs ...................................................................................................................... 27
2.5.3 Leaving Program Mode ........................................................................................................................... 27
2.6 Starting and Stopping the Centrifuge.....................................................................................................28
2.6.1 Starting the Centrifuge............................................................................................................................. 28
2.6.2 The "STOP" Key...................................................................................................................................... 28
2.7 Imbalance Detection.................................................................................................................................29
2.8 Timer-Function..........................................................................................................................................29
3. MAINTENANCE...................................................................................................................................31
3.1 Maintenance and Cleaning.......................................................................................................................31
3.1.1 General Care:.......................................................................................................................................... 31
3.1.2 Cleaning and Disinfecting of the Unit ...................................................................................................... 32
3.1.3 Cleaning and Disinfecting of the Rotor.................................................................................................... 32
3.1.4 Disinfection of Aluminum Rotors ............................................................................................................. 32
3.1.5 Disinfection of PP-Rotors ........................................................................................................................ 33
3.1.6 Glass Breakage....................................................................................................................................... 33
3.2 Lifetime of Rotors, Round and Rectangular Buckets, Accessories.....................................................33
4. TROUBLE SHOOTING........................................................................................................................34
4.1 Error Message: Problem / Solution.........................................................................................................34
4.2 Survey of Possible Error Messages and Solutions...............................................................................34
4.2.1 Lid Release during Power Failure (Emergency Lid Release).................................................................. 34
4.2.2 Description of the Error Message System............................................................................................... 35
4.2.3 Procedure while error 14, from software version 1.76............................................................................. 35
4.2.4 Procedure while error 38 –Lid motor is blocked ..................................................................................... 36
5. RECEIPT OF CENTRIFUGES FOR REPAIR ......................................................................................36
6. TRANSPORT, STORAGE AND DISPOSAL ..........................................................................................I
6.1 Transport......................................................................................................................................................I
6.2 Storage .........................................................................................................................................................I
6.3 Disposal........................................................................................................................................................I
7. APPENDIX.............................................................................................................................................II
EG - Conformity Declaration ............................................................................................................................. III
Table 1: Technical Data ....................................................................................................................................IV
Table 2: Permissible Net Weight........................................................................................................................V
Table 3: Lowest Temperature at Max. Speed ....................................................................................................V
Table 4: Max. Speed and RCF-Values for Permissible Rotors .........................................................................VI

CONTENTS
Z366K_V1.22_eng © Hermle Labortechnik GmbH
Table 5: Acceleration and Deceleration Time ...................................................................................................VI
Table 6: Error Messages..................................................................................................................................VII
Table 7 (part 1): Radius Correction.................................................................................................................VIII
Table 7 (part 2): Radius Correction...................................................................................................................IX
Table 7 (part 3): Radius Correction....................................................................................................................X
Table 8: Abbreviations .......................................................................................................................................X
Redemption Form / Decontamination Certificate .............................................................................................XII

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 4
1. PRODUCT DESCRIPTION
1.1 Safety Instructions
This symbol indicates safety instructions and points of potential dangerous situations. Before using
the centrifuge for the first time, please read the operating manual.
Failure to follow these instructions can result in personal injury and/or property damage .
Intended use includes: the observation of all instructions, in the instruction manual, and
administering inspection and maintenance.
1.2 Intended Purpose
This Hermle centrifuge was designed only for the separation of materials or mixtures with different
densities, specifically for the preparation and processing of samples, from the human body, in
context of an in-vitro-diagnostic use, to allow the use of in-vitro-diagnostic in accordance to its'
intended purpose. The designated device and its' accessories, listed in the technical documentation,
are in compliance with the Regulation (EU) 2017/746 of the European Parliament and of the Council
of 5 April 2017 on in-vitro-diagnostic medical devices.
Hermle Centrifuges are intended exclusively for indoor use and for the use of qualified personnel.
Only Hermle original rotors and accessories should be used. Any other use or intended use is strictly
prohibited. For any resulting damage, the company, Hermle Labortechnik, is not liable.
1.3 Brief Description
The unit type, Z 366 K, is a refrigerated universal centrifuge, which we offer in two voltage variations
230V or 120V.
The centrifuge can be used with swing-out rotors and angle rotors.
All parameters are accessible via buttons, and selected with a central adjuster. All pre-selected and
current values will be shown, permanently, on the LCD-Display.
The centrifuge is powered by a Maintenance-Free Induction Motor.
Detailed technical data are in Table 1: "Technical Data" (see APPENDIX P.V).
1.4 Delivery Package
•1 Centrifuge Z 366 K
•1 Operating Manual Z 366 K
•1 Rotor Key
*Rotor(s) / Accessories will be packaged separately.

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 5
1.5 Operating and Display Elements
1central adjuster Run Parameters
20-I Power Switch
3LCD Control Panel Display
4rpm/rcf Speed/ g-force
5 accel/decel Acceleration- / Deceleration Intensity
6time Centrifugation Time
7 lid Lid Release
8quick Short Running
9start Start Centrifugation
10 stop Stop Centrifugation
11 prog Retrieving Stored Programs
12 store Program Store
13 temp Temperature Indication

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 6
1.5.1 LCD-Display
The following picture shows the individual elements of the LCD-Display.
Figure 1
Display Fields:
A-1 Display Field –"rpm/rcf"
A-2 Display Field –"acc/dec"
A-3 Display Field –"time"
A-4 Display Field - "temp"
Messages/Logos of the Display Fields:
M1 "close" M8 "decel" M15 "precool"
M2 "open" M9 "radius"
M3 "rotor" M10 "program"
M4 Rotor-No. M11 "error"
M5 "rpm" M12 "service"
M6 "rcf" M13 h m s
M7 "accel" M14 "temperature"
Indication:
After switching on the centrifuge, the display, "rpm/rcf"(A-1) shows the loading status, the current
software version and finally the model type (e.g. Z 36 HK).

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 7
1.6 Signs and Indications of the Centrifuge
1.6.1 General
Instructions for Disposal (see P. 34)
Direction of Rotation –clockwise rotation for the rotor drive
Reference for Loading Rotors
1.6.2 Product Nameplate (Example)
Company address
Hermle Labortechnik GmbH, Siemensstr. 25, D-78564 Wehingen
In-Vitro-Diagnostic Medical Device
TYPE:
Type Designation of the Product
REF:
Order No. of the Product
SN:
Serial No. of the Product
Manufacturer
Date of Manufacture
MAX. Drehzahl:
Max. Speed Allowed of the Unit
KIN. EN.:
Max. Kinetic Energy with Corresponding Rotor
U/I/f:
Allowable Voltage / Max. Current / Frequency
P:
Electrical Input Power
Operating Manual Indication

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 8
Labeling, Standards and Guidelines
UK-Conformity Assessed
RoHS Conformity
1.6.3 Warning and Information Signs
Four microtiter carrier must be used at all times on four places
swing out rotors or damage will occur to the centrifuge. Such
damage will not be covered under the product warranty
Attention! Check the fastening of the rotor nut before each run
Take off mains plug before opening the housing or the emergency
release
Power Input
Rotation Direction Emergency Release
Biohazard Warning
1.6.4 Danger, Precautions and Warranty
This device may only be operated by a trained professional. Carefully, read the
operating manual and be familiar with the functions of the device.
To protect people and the environment, the following precautions must be taken:
•During centrifugation, the presence of people and the arrangement of hazardous materials is
strictly prohibited, within 30 cm/12 in around the centrifuge, according to the regulations of
EN 61010-2-020.
•The HERMLE Z 366 K, is a non "explosion-proof" and must not be operated in explosion-
endangered areas or locations. Centrifugation of flammable, explosive, radioactive, or such
substances, which chemically react with high energy, is strictly prohibited. If used in such
environment, this is at the users own expense.
•Never spin toxic or pathogenic material without adequate safety precautions, i.e.
centrifugation of buckets / tubes with or without defective hermetic sealing, is strictly
prohibited. The user is obliged to perform appropriate disinfection procedures, in case

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 9
dangerous substances have contaminated the centrifuge and/or its' accessories. When
centrifuging infectious substances, always pay attention to the General Laboratory
Precautions. If necessary, contact your safety officer!
•It is prohibited to run the centrifuge, with rotors not manufactured for this unit.
•Under no circumstances open the lid of the centrifuge, while the rotor is still running or
rotating with a speed of > 2m/s
1.6.5 Following Rules Must Strictly be Adhered To:
•Do not operate the centrifuge if not installed correctly.
•Do not operate the centrifuge when dismounted (e.g. without housing).
•Do not run the centrifuge, if mechanical or electrical assembly groups have been tampered
with, by unauthorized personnel.
•Do not use accessories such as rotors and buckets, that are not approved by HERMLE
Labortechnik GmbH, except commercially available centrifuge tubes, made of glass or
plastic.
•Do not spin extremely corrosive substances, as they may cause material damages and impair
mechanical resistance.
•Do not operate the centrifuge with rotors or buckets, that show any signs of corrosion or
mechanical damage.
The manufacturer is responsible for safety and reliability, of the centrifuge, only if:
•The unit is operated in accordance to this instruction manual.
•Modifications, repairs or other adjustments are performed by HERMLE-authorized personnel
and the electrical installation of the related location corresponds to the IEC-regulations.
1.6.6 Warranty
The centrifuge has been subjected to thorough testing and quality control. In the unlikely case of any
manufacturing faults occurring, the centrifuge and rotors are covered by warranty, for a period of two
years, from date of delivery. This warranty becomes invalid in any case of mishandling, damage
and/or negligence and further in any case of usage of inappropriate spare parts and / or accessories
or unauthorized modification of the unit.
Technical modification rights are reserved, by the manufacturer, in regards to technical
improvement!

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 10
1.7 Installation of the Centrifuge
1.7.1 Unpacking the Centrifuge
Model Z 366 Kis supplied in a carton.
Remove the strap retainer, open the carton, and remove the padding.
Lift the centrifuge on both sides (see Figure 1) with an appropriate number of helpers and place it on
the laboratory table.
Attention! Do not lift the centrifuge from under the lid or by the front panel!
Figure 1
The instruction manual must be kept with the centrifuge, at all times!
1.7.2 Space Requirements
The centrifuge should be installed on an even, solid surface, if possible on a laboratory cabinet /
table or some other solid vibration free surface.
During centrifugation, the centrifuge must be placed in a way, that there is a minimum space of
30 cm/11.81in on each side of the unit, according to EN 61010-2-020 standards.
Do not place the centrifuge next to a window or a heater where it could be disposed to excessive
heat, as the performance of the unit is based on an ambient temperature of 23°C/73.4°F.
1.7.3 Installation
Follow These Steps:
•Check whether the power supply corresponds with the one specified on the manufacturer's rating
label, mounted on the rear panel.
•The power connection for the centrifuge requires a separate one-site protection, with 16 A (Type K)
•In case of emergency, there must be an emergency switch off installed outside of the room, in order
to disconnect the power supply from the unit.
•Connect the centrifuge, with the mains.
(The socket for the power cord must be easy to reach, respectively easy to disconnect).
Switch on, by using the mains power switch (I).
Open the lid, by using the button LID.
• Remove the transport securing device of the motor.

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 11
1.7.4 Attention before first use!
Depending on how the motor shaft of your centrifuge is looking like, some action must be taken
before the first operation. Please note the shape of the motor shaft of your centrifuge.
There are two different versions:
Version 1 Version 2
If the motor shaft is conical
remove the collet from the rotor by turning it
counter clockwise
The collet is no longer
needed
If the motor shaft is cylindrical
Let the collet inside the rotor
Conical
motor shaft
Cylindrical
motor shaft

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 12
1.8 Basic Adjustments
At commissioning of the centrifuge, you have the options to make the following basic changes:
-Temperature Indication in °C or °F
-Acoustic Signal Turn on / off
-Keyboard Sound Turn on / off
-Volume Pre-Selection of Sound Signal
-Song Selection of Sound Signal "end of run"
1.8.1 Access to Mode: "Operating Data"
If the centrifuge is still turned off, press the keys, "time" (6) and "lid" (7) simultaneously, turning on
the main switch of the centrifuge. Now release both keys. As a result, a display test is administered
for approx. 5 seconds. All possible indications will appear at the same time (see Figure 2).
Figure 2
ATTENTION:
-Please notice that you must enter the program as described, under point 1.8.1,to
change the adjustments of the points 1.8.2 - 1.8.6. After the settings have been
stored by user, the normal program mode can be changed back again by
switching off the centrifuge, for a short period.
-All changed settings must be confirmed by the key, "start" (9). A confirmation
screen will appear with the word; "store" in the display box, "rpm/rcf"(A-1) - Only
then, the pre-selections are valid, (see Figure 3)!
Figure 3
1
7

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 13
1.8.2 Temperature Indication
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key "accel/decel" (5).
In the display, "accel/decel" (A-2) appears the word, "service". Select the letter, "C", with the
adjustable knob (1). As a result, appearing in the display, "rpm/rcf" (A-1) are the words, "CELSI/temp".
By pressing the key, "rpm/rcf" (4), the word "CELSI" flashes, this allows you to change the display into
Fahrenheit "FAREN", with the adjustable knob (1), (see Figure 4).
After the settings have been stored by user, the normal program mode can be changed back again by
switching off the centrifuge, for a short period.
Figure 4
1.8.3 Sound Signal Turn On / Off
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key; "accel/decel"
(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter, "L" with the
adjustable knob (1). As a result, appearing in the display "rpm/rcf" (4), are the words, "On Sound".
By pressing the key, "rpm/rcf" (4), the word "On" flashes, and the sound can be switched off with the
adjustable knob (1), (see Figure 5).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
Figure 5
2
3
2
3

PRODUCT DESCRIPTION
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1.8.4 Volume Pre-Selection of Sound Signal
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key "accel/decel" (5).
In the display, "accel/decel" (A-2), flashes the word, "service". Select the letter, "U" with the
adjustable knob (1). As a result, appearing in the display, "rpm/rcf" (A-1), are the words, "Vol=0-
9/Sound". By pressing the key, "rpm/rcf" (4), the desired volume can be adjusted between 0 (low)
and 9 (loud), with the adjustable knob (1), (see Figure 6).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
Figure 6
1.8.5 Song Selection- End of Run
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key, "accel/decel"
(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter, "G" with the
adjustable knob (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word " SonGo/Sound".
After pressing the key „rpm/rcf“ (4), select a song with the adjusting knob (1), (see Figure 7).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
Figure 7
9
8
1
7

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 15
1.8.6 Keyboard Sound Turn On / Off
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key, "accel/decel"
(5). In the display; "accel/decel" (A-2) flashes the word, "service". Select the letter, "b" with the
adjustable knob (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word "ON/BEEP". By
pressing the key, "rpm/rcf" (4), the keyboard sound (On) or (Off) can be turned on, with the
adjustable knob (1), (see Figure 8).
After the settings have been stored by user (see 1.8.1), the normal program mode can be changed
back again, by switching off the centrifuge, for a short period.
Figure 8

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 16
1.8.7 Retrieving Operating Data (operated by skilled or service engineer only!)
In the function, "Basic Adjustments", retrieving the operating data of the centrifuge is an available
option. Please proceed as illustrated, under point 1.8.1, to enter this program mode, press the key,
"accel/decel" (5). In the display, "accel/decel" (A-2) flashes the word, "service".
With the adjustable knob (1), the following information can be retrieved:
A = Previous Starts of the Centrifuge
H = Previous Operating Hours
S = Software Version
r = Converter Software
E = List of Previous Error Messages
h = Running Time of the Motor
The list of the last 99 error messages can be reviewed by pressing the key, "rpm/rcf" (4) and scroll
through, with the adjustable knob (1). The respective error codes appear in the display, "rpm/rcf" (A-
1). Please refer to Table 6: "Error Messages“, (see APPENDIX P.VIII).
Switch off the centrifuge, to return to the normal program mode.
Figure 9

OPERATION
© Hermle Labortechnik GmbH Z366K_V1.22_eng 17
2. OPERATION
2.1 Mounting and Loading the Angle Rotor
2.1.1 Installation of Rotors
Clean the drive shaft and the collet with a clean, grease-free piece of cloth. Place the rotor onto the
drive shaft, (see Figure 10). Please be sure that the rotor is fully installed onto the motor shaft.
Figure 10
Hold the rotor with one hand and secure the rotor to the shaft, by turning the fixing nut clockwise.
Tighten the fixing nut with the provided Allen key, (see Figure 11).
Figure 12
ATTENTION: For safety, always ensure that the rotor fixing screw is tightened before
each run, (see Figure 11)!!
Figure 11

OPERATION
18 Z366K_V1.22_eng © Hermle Labortechnik GmbH
2.1.2 Loading Angle Rotors
Rotors must be loaded symmetrically and with equal weight. The adapter may only be loaded with
the appropriate vessels. The weight differences between the filled vessels should be kept as low as
possible. Therefore, we recommend to weigh with a balance. This reduces the wear of drive and the
acoustic operating noise.
Each rotor indicates what the maximum capacity is per hole.
Attention:
For safety reasons, all places on certain rotors must be occupied with equal weight during
centrifugation (See Figure 14).
Figure 13: WRONG Figure 14: RIGHT (4 tubes)
Regardless of the centrifuge model, this applies to the following angular rotors:
221.51 (4 x 500 ml)
221.21 (6 x 250 ml)
220.78 (6 x 85 ml)
221.18 (6 x 85 ml)
221.20 (4 x 85 ml)
2.1.3 Loading Swing Out Rotors
Loading of the buckets / vessels must be done in accordance, to Figure 16.
It is allowed to operate e.g. a 4-place-rotor with 2 loaded buckets only. The loaded buckets must be
opposite of each other. Make sure that the unloaded buckets are placed inside the rotor,
(see Figures 15 and 16).
In principle, swing out rotors cannot be removed during operation, until all buckets or racks
are placed inside the rotor.
The bolts of the rotor must be greased with the HERMLE Rotorgrease (Order No. 38-5656).
The sample tubes have to be filled evenly, by eye, and set into the drillings or tube racks. The weight
difference of the loaded buckets should not exceed 1.0 g.
ATTENTION!
Swing out Rotors can be removed during operation, only if all locations are filled in with
either 4 buckets or 4 microtiter carriers –do not mix up buckets and microtiter carriers!