Hermle Z 366 User manual

© Hermle Labortechnik GmbH Z366_V1.18_eng
Operating Manual for
Universal
Table Top Centrifuge
Z 366

CONTENTS
© Hermle Labortechnik GmbH Z366_V1.18_eng I
1. PRODUCT DESCRIPTION ..................................................................................................1
1.1 Safety Instructions ............................................................................................................................................ 1
1.2 Intended Purpose .............................................................................................................................................. 1
1.3 Brief Description................................................................................................................................................ 1
1.4 Delivery Package ............................................................................................................................................... 1
1.5 Operating and Display Elements...................................................................................................................... 2
1.5.1 LCD-Display .................................................................................................................................................... 3
1.6 Signs and Indications of the Centrifuge.......................................................................................................... 4
1.6.1 General............................................................................................................................................................ 4
1.6.2 Product Nameplate (Example) ........................................................................................................................ 4
1.6.3 Warning and Information Signs....................................................................................................................... 5
1.6.4 Danger, Precautions and Warranty ................................................................................................................. 5
1.6.5 Following Rules Must Strictly be Adhered To:................................................................................................. 5
1.6.6 Warranty.......................................................................................................................................................... 6
1.7 Installation of the Centrifuge ............................................................................................................................ 7
1.7.1 Unpacking the Centrifuge................................................................................................................................ 7
1.7.2 Space Requirements....................................................................................................................................... 7
1.7.3 Installation ....................................................................................................................................................... 7
1.7.4 Attention before first use!................................................................................................................................. 8
1.8 Basic Adjustments ............................................................................................................................................ 9
1.8.1 Access to Mode: "Operating Data".................................................................................................................. 9
1.8.2 Sound Signal Turn On / Off ........................................................................................................................... 10
1.8.3 Volume Pre-Selection of Sound Signal ......................................................................................................... 10
1.8.4 Song Selection - End of Run......................................................................................................................... 11
1.8.5 Keyboard Sound Turn On / Off...................................................................................................................... 11
1.8.6 Retrieving Operating Data (operated by skilled or service engineer only!).................................................... 12
2. OPERATION......................................................................................................................13
2.1 Mounting and Loading the Angle Rotor ........................................................................................................ 13
2.1.1 Installation of Rotors...................................................................................................................................... 13
2.1.2 Loading Angle Rotors.................................................................................................................................... 13
2.1.3 Loading Swing Out Rotors............................................................................................................................. 14
2.1.4 Loading and Overloading of Rotors............................................................................................................... 15
2.1.5 Removing the Rotor ...................................................................................................................................... 15
2.2 Lid ..................................................................................................................................................................... 16
2.2.1 Lid Release ................................................................................................................................................... 16
2.2.2 Lid Lock......................................................................................................................................................... 16
2.3 Pre-Selection.................................................................................................................................................... 17
2.3.1 Pre-Selection of Speed / RCF-Value............................................................................................................. 17
2.3.2 Pre-Selection of Running Time...................................................................................................................... 17
2.3.3 Pre-Selection of Brake Intensity and Acceleration......................................................................................... 18
2.4 Radius Correction............................................................................................................................................ 19
2.5 Program............................................................................................................................................................ 19
2.5.1 Storage of Programs ..................................................................................................................................... 19

CONTENTS
II Z366_V1.18_eng © Hermle Labortechnik GmbH
2.5.2 Recall of Stored Programs ............................................................................................................................ 20
2.5.3 Leaving Program Mode................................................................................................................................. 21
2.6 Starting and Stopping the Centrifuge............................................................................................................ 21
2.6.1 Starting the Centrifuge................................................................................................................................... 21
2.6.2 The "STOP" Key............................................................................................................................................ 22
2.7 Imbalance Detection........................................................................................................................................ 23
3. MAINTENANCE.................................................................................................................24
3.1 Maintenance and Cleaning.............................................................................................................................. 24
3.1.1 General Care:................................................................................................................................................ 24
3.1.2 Cleaning and Disinfecting of the Unit ............................................................................................................ 25
3.1.3 Cleaning and Disinfecting of the Rotor.......................................................................................................... 25
3.1.4 Disinfection of Aluminum Rotors ................................................................................................................... 25
3.1.5 Disinfection of PP-Rotors .............................................................................................................................. 25
3.1.6 Glass Breakage............................................................................................................................................. 26
3.2 Lifetime of Rotors, Round and Rectangular Buckets, Accessories............................................................ 26
4. TROUBLE SHOOTING ......................................................................................................27
4.1 Error Message: Problem / Solution................................................................................................................ 27
4.2 Survey of Possible Error Messages and Solutions ...................................................................................... 27
4.2.1 Lid Release during Power Failure (Emergency Lid Release)........................................................................ 27
4.2.2 Description of the Error Message System..................................................................................................... 28
4.2.3 Procedure while error 14, from software version 1.76................................................................................... 28
5. RECEIPT OF CENTRIFUGES FOR REPAIR.....................................................................29
6. TRANSPORT, STORAGE AND DISPOSAL......................................................................30
6.1 Transport.......................................................................................................................................................... 30
6.2 Storage ............................................................................................................................................................. 30
6.3 Disposal............................................................................................................................................................ 30
7. APPENDIX..........................................................................................................................III
EG - Conformity Declaration ...................................................................................................................................IV
Table 1: Technical Data ...........................................................................................................................................V
Table 2: Permissible Net Weight.............................................................................................................................VI
Table 3: Max. Speed and RCF-Values for Permissible Rotors ...............................................................................VI
Table 4: Acceleration and Deceleration Times.......................................................................................................VII
Table 5: Error Messages.......................................................................................................................................VIII
Table 6 (Part 1): Radius Correction.........................................................................................................................IX
Table 6 (Part 2): Radius Correction..........................................................................................................................X
Table 6( Part 3): Radius Correction.........................................................................................................................XI
Table 7: Abbreviations ............................................................................................................................................XI
Redemption Form / Decontamination Certificate ...................................................................................................XII
8. NOTES.............................................................................................................................XIV

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366_V1.18_eng 1
1. PRODUCT DESCRIPTION
1.1 Safety Instructions
This symbol indicates safety instructions and points of potential dangerous situations. Before using
the centrifuge for the first time, please read the operating manual.
Failure to follow these instructions can result in personal injury and/or property damage .
Intended use includes: the observation of all instructions, in the instruction manual, and
administering inspection and maintenance.
1.2 Intended Purpose
This Hermle centrifuge was designed only for the separation of materials or mixtures with different
densities, specifically for the preparation and processing of samples, from the human body, in
context of an in-vitro-diagnostic use, to allow the use of in-vitro-diagnostic in accordance to its'
intended purpose. The designated device and its' accessories listed, in the technical documentation,
are in compliance with Directive 98/79/EC for In-Vitro-Diagnostic Medical Devices.
Hermle Centrifuges are intended exclusively for indoor use and for the use of qualified personnel.
Only Hermle original rotors and accessories should be used. Any other use or intended use is strictly
prohibited. For any resulting damage, the company, Hermle Labortechnik, is not liable.
1.3 Brief Description
The unit type Z 366, is a non refrigerated universal centrifuge, which we offer in two voltage
variations 230V or 120V.
The centrifuge can be used with swing-out rotors and angle rotors.
All parameters are accessible via buttons, and selected with a central adjuster. All pre-selected and
current values will be shown, permanently, on the LCD-Display.
The centrifuge is powered by a Maintenance-Free Induction Motor.
Detailed technical data are in Table 1: "Technical Data" (see APPENDIX P.V).
1.4 Delivery Package
•1 Centrifuge Z 366
•1 Operating Manual Z 366
•1 Rotor key
*Rotor(s) / Accessories will be packaged separately.

PRODUCT DESCRIPTION
2 Z366_V1.18_eng © Hermle Labortechnik GmbH
1.5 Operating and Display Elements
1central adjuster Run Parameters
20-I Power Switch
3LCD Control Panel Display
4rpm/rcf Speed/ g-Force
5 accel/decel Acceleration / Deceleration Intensity
6time Centrifugation Time
7 lid Lid Release
8quick Short Running
9start Start Centrifugation
10 stop Stop Centrifugation
11 prog Retrieving Stored Programs
12 store Program Store
11 12 1
8
10
3
4
5
6
7
9
2

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366_V1.18_eng 3
1.5.1 LCD-Display
The following picture shows the individual elements of the LCD-display.
Figure 1
Display Fields:
A-1 Display Field –"rpm/rcf"
A-2 Display Field –"acc/dec"
A-3 Display Field –"time"
Messages/Logos of the Display Fields:
M1 "close" M8 "decel"
M2 "open" M9 "radius"
M3 "rotor" M10 "program"
M4 Rotor-No. M11 "error"
M5 "rpm" M12 "service"
M6 "rcf" M13 h m s
M7 "accel"
Indication:
After switching on the centrifuge, the display, "rpm/rcf"(A-1) shows the loading status, the current
software version and finally the model type (e.g. Z 36 HK).

PRODUCT DESCRIPTION
4 Z366_V1.18_eng © Hermle Labortechnik GmbH
1.6 Signs and Indications of the Centrifuge
1.6.1 General
Instructions for Disposal (see P. 28)
Direction of Rotation –clockwise rotation for the rotor drive
Reference for Loading Rotors
1.6.2 Product Nameplate (Example)
Company Address: Hermle Labortechnik GmbH, Siemensstr. 25, D-78564 Wehingen
TYPE: Type Designation of the Product
REF: Order No. of the Product
SN: Serial Number of the Product
Date of Manufacture
Manufacturer
MAX. Drehzahl: Max. Allowed Speed of the Unit
KIN. EN.: Max. Kinetic Energy with Corresponding Rotor
U/I/f: Allowable Voltage / Max. Current / Frequency
P: Electrical Input Power
Operating Manual!
Labeling, Standards and Guidelines

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366_V1.18_eng 5
1.6.3 Warning and Information Signs
Four carrier must be used at all times on four place swing out rotors or
damage will occur to the centrifuge. Such damage will not be covered
under the product warranty.
Attention! Check the fastening of the rotor nut before each run.
Take off mains plug before opening the housing or the emergency
release
Power Input
1.6.4 Danger, Precautions and Warranty
This device may only be operated by a trained professional. Carefully, read the
operating manual and be familiar with the functions of the device.
To protect people and the environment, the following precautions must be taken:
•During centrifugation, the presence of people and the arrangement of hazardous materials is
strictly prohibited, within 30 cm/12 in around the centrifuge, according to the regulations of
EN 61010-2-020.
•The HERMLE Z 366 is "non explosion-proof" and must not be operated in explosion-
endangered areas or locations. Centrifugation of flammable, explosive, radioactive, or such
substances, which chemically react with high energy, is strictly prohibited. If used in such
environment, this is at the users own expense.
•Never spin toxic or pathogenic material without adequate safety precautions, i.e.
centrifugation of buckets / tubes with or without defective hermetic sealing, is strictly
prohibited. The user is obliged to perform appropriate disinfection procedures, in case
dangerous substances have contaminated the centrifuge and/or its' accessories. When
centrifuging infectious substances, always pay attention to the General Laboratory
Precautions. If necessary, contact your safety officer!
•It is prohibited to run the centrifuge, with rotors not manufactured for this unit.
•Under no circumstances open the lid of the centrifuge, while the rotor is still running or
rotating with a speed of > 2m/s
1.6.5 Following Rules Must Strictly be Adhered To:
•Do not operate the centrifuge if not installed correctly.

PRODUCT DESCRIPTION
6 Z366_V1.18_eng © Hermle Labortechnik GmbH
•Do not operate the centrifuge when dismounted (e.g. without housing).
•Do not run the centrifuge, if mechanical or electrical assembly groups have been tampered
with, by unauthorized personnel.
•Do not use accessories such as rotors and buckets, that are not approved by HERMLE
Labortechnik GmbH, except commercially available centrifuge tubes, made of glass or
plastic.
•Do not spin extremely corrosive substances, as they may cause material damages and impair
mechanical resistance.
•Do not operate the centrifuge with rotors or buckets, that show any signs of corrosion or
mechanical damage.
The manufacturer is responsible for safety and reliability, of the centrifuge, only if:
•The unit is operated in accordance to this instruction manual.
•Modifications, repairs or other adjustments are performed by HERMLE-authorized personnel
and the electrical installation of the related location corresponds to the IEC-regulations.
1.6.6 Warranty
The centrifuge has been subjected to thorough testing and quality control. In the unlikely case of any
manufacturing faults occurring, the centrifuge and rotors are covered by warranty, for a period of two
years, from date of delivery. This warranty becomes invalid in any case of mishandling, damage
and/or negligence and further in any case of usage of inappropriate spare parts and / or accessories
or unauthorized modification of the unit.
Technical modification rights are reserved, by the manufacturer, in regards to technical
improvement!

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366_V1.18_eng 7
1.7 Installation of the Centrifuge
1.7.1 Unpacking the Centrifuge
Model Z 366 is supplied in a carton.
Remove the strap retainer, open the carton, and remove the padding.
Lift the centrifuge on both sides (see Figure 1) with an appropriate number of helpers and place it on
the laboratory table.
Attention! Do not lift the centrifuge from under the lid or by the front panel!
Figure 1
The instruction manual must be kept with the centrifuge, at all times!
1.7.2 Space Requirements
The centrifuge should be installed on an even, solid surface, if possible on a laboratory cabinet /
table or some other solid vibration free surface.
During centrifugation, the centrifuge must be placed in a way, that there is a minimum space of
30 cm/11.81in on each side of the unit, according to EN 61010-2-020 standards.
Do not place the centrifuge next to a window or a heater where it could be disposed to excessive
heat, as the performance of the unit is based on an ambient temperature of 23°C/73.4°F.
1.7.3 Installation
Follow These Steps:
•Check whether the power supply corresponds with the one specified on the manufacturer's
rating label, mounted on the rear panel.
•The power connection for the centrifuge requires a separate one-site protection, with 16 A (Type K)
•In case of emergency, there must be an emergency switch off installed outside of the room, in order
to disconnect the power supply from the unit.
•Connect the centrifuge, with the mains.
(The socket for the power cord must be easy to reach, respectively easy to disconnect).
Switch on, by using the mains power switch (I).
Open the lid, by using the button LID.
• Remove the transport securing device of the motor.

PRODUCT DESCRIPTION
8 Z366_V1.18_eng © Hermle Labortechnik GmbH
1.7.4 Attention before first use!
Depending on how the motor shaft of your centrifuge is looking like, some action must be taken
before the first operation. Please note the shape of the motor shaft of your centrifuge.
There are two different versions:
Version 1 Version 2
If the motor shaft is conical
remove the collet from the rotor by turning it
counter clockwise
The collet is no longer
needed
If the motor shaft is cylindrical
Let the collet inside the rotor
Conical
motor shaft
Cylindrical
motor shaft

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366_V1.18_eng 9
1.8 Basic Adjustments
At commissioning of the centrifuge, you have the options to make the following basic changes:
-Acoustic Signal Turn On / Off
-Keyboard Sound Turn On / Off
-Volume Pre-Selection of Sound Signal
-Song Selection - "End of Run"
1.8.1 Access to Mode: "Operating Data"
If the centrifuge is still turned off, press the keys, "time" (6) and "lid" (7) simultaneously, turning on
the main switch of the centrifuge. Now release both keys. As a result, a display test is administered
for approx. 5 seconds. All possible indications will appear at the same time (see Figure 2).
Figure 2
Attention:
-Please notice that you must enter the program as described, under point 1.8.1,to
change the adjustments of the points 1.8.2 - 1.8.6. After the settings have been
stored by user, the normal program mode can be changed back again by
switching off the centrifuge, for a short period.
-All changed settings must be confirmed by the key, "start" (9). A confirmation
screen will appear with the word; "store" in the display box, "rpm/rcf"(A-1) - Only
then, the pre-selections are valid, (see Figure 3)!

PRODUCT DESCRIPTION
10 Z366_V1.18_eng © Hermle Labortechnik GmbH
Figure 3
1.8.2 Sound Signal Turn On / Off
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key; "accel/decel"
(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter, "L" with the
adjustable knob (1). As a result, appearing in the display "rpm/rcf" (4), are the words, "On Sound".
By pressing the key, "rpm/rcf" (4), the word "On" flashes, and the sound can be switched off with the
adjustable knob (1), (see Figure 4).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
Figure 4
1.8.3 Volume Pre-Selection of Sound Signal
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key "accel/decel" (5).
In the display, "accel/decel" (A-2), flashes the word, "service". Select the letter, "U" with the
adjustable knob (1). As a result, appearing in the display, "rpm/rcf" (A-1), are the words, "Vol=0-
9/Sound". By pressing the key, "rpm/rcf" (4), the desired volume can be adjusted between 0 (low)
and 9 (loud), with the adjustable knob (1), (see Figure 5).
After the settings have been stored by user (see 1.8.1), the normal program mode can be changed
back again by switching off the centrifuge, for a short period.
2
3
1
7

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z366_V1.18_eng 11
Figure 5
1.8.4 Song Selection - End of Run
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key, "accel/decel"
(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter, "G" with the
adjustable knob (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word " SonGo/Sound".
After pressing the key „rpm/rcf“ (4), select a song with the adjusting knob (1), (see Figure 6).
After the settings have been stored by user (see 1.8.1), the normal program mode can be changed
back again by switching off the centrifuge, for a short period.
Figure 6
1.8.5 Keyboard Sound Turn On / Off
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key, "accel/decel"
(5). In the display; "accel/decel" (A-2) flashes the word, "service". Select the letter, "b" with the
adjustable knob (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word "ON/BEEP". By
pressing the key, "rpm/rcf" (4), the keyboard sound (On) or (Off) can be turned on, with the
adjustable knob (1), (see Figure 7).
After the settings have been stored by user (see 1.8.1), the normal program mode can be changed
back again, by switching off the centrifuge, for a short period.
9
8
1
7

PRODUCT DESCRIPTION
12 Z366_V1.18_eng © Hermle Labortechnik GmbH
Figure 7
1.8.6 Retrieving Operating Data (operated by skilled or service engineer only!)
In the function, "Basic Adjustments", retrieving the operating data of the centrifuge is an available
option. Please proceed as illustrated, under point 1.8.1, to enter this program mode, press the key,
"accel/decel" (5). In the display, "accel/decel" (A-2) flashes the word, "service".
With the adjustable knob (1), the following information can be retrieved:
A = Previous Starts of the Centrifuge
H = Previous Operating Hours
S = Software Version
r = Converter Software
E = List of Previous Error Message
h = Running Time of the Motor
The list of the last 99 error messages can be reviewed by pressing the key, "rpm/rcf" (4) and scroll
through, with the adjustable knob (1). The respective error codes appear in the display, "rpm/rcf" (A-
1). Please refer to Table 6: "Error Messages“, (see APPENDIX P.VIII).
Switch off the centrifuge, to return to the normal program mode.
Figure 8

OPERATION
© Hermle Labortechnik GmbH Z366_V1.18_eng 13
2. OPERATION
2.1 Mounting and Loading the Angle Rotor
2.1.1 Installation of Rotors
Clean the drive shaft and the collet with a clean, grease-free piece of cloth. Place the rotor onto the
drive shaft, (see Figure 9). Please be sure that the rotor is fully installed onto the motor shaft.
Figure 9
Hold the rotor with one hand and secure the rotor to the shaft, by turning the fixing nut clockwise.
Tighten the fixing nut with the provided Allen key, (see Figure 10)
Figure 11
ATTENTION: For safety, always ensure that the rotor fixing screw is tightened before
each run, (see Figure 10)!!
2.1.2 Loading Angle Rotors
Rotors must be loaded symmetrically and with equal weight, (see Figures 12 and 13). The adapter
may only be loaded with the appropriate vessels. The weight differences between the filled vessels
should be kept as low as possible. Therefore, we recommend to weigh with a balance. This reduces
the wear of drive and the acoustic operating noise.
Each rotor indicates what the maximum capacity is per hole. (It is allowed to operate e.g. a 12-place-
rotor with 2 or 4 loaded tubes only, but the loaded borings must be opposite of each other).
Figure 10

OPERATION
14 Z366_V1.18_eng © Hermle Labortechnik GmbH
Figure 12: WRONG Figure 13: RIGHT (4 tubes)
2.1.3 Loading Swing Out Rotors
Loading of the buckets / vessels must be done in accordance, to Figure 15.
It is allowed to operate e.g. a 4-place-rotor with 2 loaded buckets only. The loaded buckets must be
opposite of each other. Make sure that the unloaded buckets are placed inside the rotor,
(see Figures 14 and 15).
In principle, swing out rotors cannot be removed during operation, until all buckets or racks
are placed inside the rotor.
The bolts of the rotor must be greased with the HERMLE Rotorgrease (Order No. 38-5656).
The sample tubes have to be filled evenly, by eye, and set into the drillings or tube racks. The weight
difference of the loaded buckets should not exceed 1.0 g.
ATTENTION!
Swing Out Rotors can be removed during operation, only if all locations are filled in with either
4 buckets or 4 carriers –do not mix up buckets and carriers!!
Figure 14: WRONG Figure 15: RIGHT
ATTENTION!
Do not operate the centrifuge with rotors or buckets that show any signs of corrosion
or mechanical damage.
Do not operate with extremely corrosive substances, which could damage the rotor
and buckets.
In case of any questions, please contact the manufacturer!

OPERATION
© Hermle Labortechnik GmbH Z366_V1.18_eng 15
2.1.4 Loading and Overloading of Rotors
All approved rotors are listed with their maximum speed and maximum filling weight, in
Table 2: "Permissible Net Weight", (see APPENDIX P. VI).
The maximum load permitted for a rotor is determined by the manufacturer, as well as the maximum
speed allowed for this rotor (see label on rotor), must not be exceeded. The liquid the rotors are
loaded with, should have a max. homogeneous density of 1.2 g/ml or less when the rotor is running
at maximum speed.
In order to spin liquids with a higher density, the speed has to be reduced, according to the following
formula:
Reduced speed nred =
 x max. speed (nmax) of the rotor
Example:
nred = 
 x 4.000 = 3.360 rpm
In case of any questions, please contact the manufacturer!
2.1.5 Removing the Rotor
Completely, untighten the rotor fixing nut (2. screw over the stiff point) and lift the rotor vertically out of
the centrifuge, (see Figures 9 and 10).

OPERATION
16 Z366_V1.18_eng © Hermle Labortechnik GmbH
2.2 Lid
2.2.1 Lid Release
After the run, properly close the lid of the centrifuge, appearing in the display, "rpm/rcf"(A-1) with the
word, "close" (M1). If there is a rotor in the centrifuge, the word, "rotor" (M3) appears, as well as the
code number of the specified rotor, which is in the centrifuge, for example "221.28" (M4). If there is
no rotor in the centrifuge, it flashes the word, "rotor" (M3) and an additional word, "no" (M4). ). By
pressing the key, "lid" (7), the lid of centrifuge can be released. As soon as the electromagnetic lid is
completely released, the word, "open" (M2) appears. The lid of the centrifuge is now able to be
opened.
For all number marked text, please refer to Figure 16.
Figure 16
During the run, you can retrieve the rotor type at any time, by pressing the key, “lid” (7).
2.2.2 Lid Lock
The lid must only be closed slightly. An electromagnetic lid lock closes the lid, the word "open" (M2)
will no longer be displayed.
As a sign that the centrifuge is ready for starting, appearing in the display, "rpm/rcf"(A-1), the word
"close" (M1). Simultaneously, the word "rotor" (M3) appears, as well as the code number of the
rotor, which is in the centrifuge, i. e. "nr 22x.xx“ (M4), along with all rotor specific data, for example:
max. speed, acceleration etc., are available.
For all number marked text, please refer to Figure 16.
ATTENTION: Don't grip your fingers between the lid and the device or the locking
mechanism, when closing the lid!