Beckman Coulter Avanti J-HC User manual

PN J3HC-IM-5AD
December 2016
Instructions For Use
Avanti J-HC
High-Capacity Centrifuge
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Avanti J-HC
High Capacity Centrifuge
PN J3HC-IM-5AD (December 2016)
© 2016 Beckman Coulter, Inc.
All rights reserved
Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Find us on the World Wide Web at:
www.beckmancoulter.com
Beckman Coulter Eurocenter S.A.
22, rue Juste-Olivier
Case Postale 1044
CH - 1260 Nyon 1, Switzerland
Tel: [phone]
Printed in U.S.A.

PN J3HC-IM-5AD iii
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to
how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Warning, Caution, Important, and Note
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
NOTE
NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety During Installation and/or Maintenance
This centrifuge is designed to be installed by a Beckman Coulter field service representative.
Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty
covering the instrument.
Any servicing of this equipment that requires removal of any covers can expose parts which involve
the risk of electric shock or personal injury. Make sure that the power switch is turned off and the
instrument is disconnected from the main power source by removing the Mains (power) plug from
the outlet receptacle, and refer such servicing to qualified personnel.
Be sure to use the anchoring system to secure the centrifuge in place. The anchoring system is
designed to reduce the possibility of injury or damage which could result from instrument
movement in the event of a major rotor mishap.
Do not replace any centrifuge components with parts not specified for use on this instrument.

PN J3HC-IM-5AD
iv
Safety Notice
Electrical Safety
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three or five-wire electrical cord and
plug to connect this equipment to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the
centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the centrifuge and damage electrical or mechanical components.
Safety Against Risk of Fire
This centrifuge is not designed for use with materials capable of developing flammable or explosive
vapors or hazardous chemical reactions. Do not centrifuge such materials (for example, chloroform
or ethyl alcohol) in this instrument nor handle or store them near the centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the Beckman Coulter rotors and accessories designed for use in this centrifuge.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop a rotor by hand.
• Do not lift or move the centrifuge while a rotor is spinning.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Do not lean on the centrifuge or place items on it while it is operating.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this centrifuge unless all necessary safety
precautions are taken.
• Observe all cautionary information printed on the original solution containers prior to
their use.

PN J3HC-IM-5AD v
Safety Notice
RoHS Notice
• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require
more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the instrument and accessories before requesting service by
Beckman Coulter Field Service.
RoHS Notice
These labels and materials declaration table (the Table of Hazardous Substance’s Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.
China RoHS Caution Label — This label indicates that the electronic information product contains
certain toxic or hazardous substances. The center number is the Environmentally Friendly Use
Period (EFUP) date, and indicates the number of calendar years the product can be in operation.
Upon the expiration of the EFUP, the product must be immediately recycled. The circling arrows
indicate the product is recyclable. The date code on the label or product indicates the date of
manufacture.
China RoHS Environmental Label — This label indicates that the electronic information product does
not contain any toxic or hazardous substances. The center “e” indicates the product is
environmentally safe and does not have an Environmentally Friendly Use Period (EFUP) date.
Therefore, it can safely be used indefinitely. The circling arrows indicate the product is recyclable.
The date code on the label or product indicates the date of manufacture.

PN J3HC-IM-5AD
vi
Safety Notice
RoHS Notice

vii
Contents
Safety Notice,iii
Alerts for Warning, Caution, Important, and Note,iii
Safety During Installation and/or Maintenance,iii
Electrical Safety,iv
Safety Against Risk of Fire,iv
Mechanical Safety,iv
Chemical and Biological Safety,iv
RoHS Notice,v
Introduction,xiii
Certification, xiii
Scope of Manual, xiii
Conventions,xiii
Recycling Label,xiv
Multi Compliance Label,xiv
CHAPTER 1: Description,1-1
Introduction,1-1
Centrifuge Function and Safety Features,1-1
Centrifuge Function,1-1
Safety Features,1-1
Centrifuge Chassis,1-2
Housing and Door,1-2
Rotor Chamber,1-3
Friction Reduction System (FRS),1-3
Temperature Control System,1-3
Overtemp System,1-3
Drive,1-4
Name Rating Plate,1-4
Controls and Indicators,1-4
Power Switch,1-4
Control Panel,1-4
Keypad,1-7
System Keys,1-8

viii
Contents
Specifications,1-8
Available Rotors,1-10
CHAPTER 2: Operation,2-1
Introduction,2-1
Summary of Avanti J-HC Run Procedures,2-1
Manual Run,2-1
Programmed Run,2-2
Preparation,2-3
Installing the Rotor,2-4
Manual Operation,2-4
Selecting a Rotor,2-5
Entering Run Speed,2-5
Entering Run Time,2-7
Entering Run Temperature,2-10
Entering Acceleration and Deceleration Times,2-11
Starting a Run,2-12
Changing Parameters During a Run,2-13
Ending a Run,2-13
Programmed Operation,2-13
Creating a New Program,2-13
Modifying a Program,2-15
Running a Program,2-16
CHAPTER 3: Troubleshooting,3-1
Introduction,3-1
User Messages,3-1
Accessing the Rotor in Case of Power Failure,3-4
CHAPTER 4: Care and Maintenance,4-1
Introduction,4-1
Maintenance,4-1
Cleaning,4-2
Decontamination,4-3
Sterilization and Disinfection,4-3
Replacing the Air Filter,4-3
Circuit Breaker and Fuses,4-4
Speed Calibration,4-5
Speed Verification,4-5
Storage and Transport,4-6
Storage,4-6
Returning a Centrifuge,4-6

ix
Contents
Supply List,4-6
Replacement Parts and Supplies,4-7
APPENDIX A: Preinstallation Requirements,A-1
Introduction,A-1
Site Preparation Requirements,A-1
Electrical Requirements,A-1
Single- and Three-Phase Power Connections,A-2
Additional Requirements for Three-Phase Power Connections,A-3
Space Requirements,A-4
Securing the Centrifuge to the Floor,A-6
Using the JA-10 Rotor,A-6
Checking for Rotor Pins,A-6
APPENDIX B: Temperature Calibration Procedure,B-1
Description,B-1
Beckman Coulter, Inc.
Avanti J-HC Centrifuge Warranty
Related Documents

x
Illustrations
Illustrations
1.1 The Control Panel,1-5
1.2 Actual Value Display,1-5
1.3 The Setup Screen,1-6
2.1 Arranging Loads in Six-Place Swinging Bucket Rotors,2-3
3.1 Emergency Door Release Latch Access,3-5
3.2 Manual Door Release,3-6
3.3 Venting the Chamber Vacuum,3-7
3.4 Front Panel Retaining Strip,3-9
A.1 Avanti J-HC Single-Phase Power Connections,A-2
A.2 Avanti J-HC Three-Phase “Y” Power Connections,A-3
A.3 Correct and Incorrect Three-Phase Power Configurations,A-4
A.4 Rear View and Dimensions,A-5
A.5 Checking the Rotor for Drive Pins,A-7

xi
Tables
Tables
1.1 Complete and Abbreviated Rotor Names,1-6
1.2 Available Rotors for the Avanti J-HC Centrifuge,1-11
2.1 Acceleration and Deceleration Settings,2-11
3.1 Diagnostic Message Chart,3-2
A.1 Nominal Supply Voltage Ratings for the Avanti J-HC,A-2
A.2 Required Wire Connections,A-3

xii
Tables

PN J3HC-IM-5AD xiii
Introduction
Certification
Beckman Coulter Avanti J-HC centrifuges are manufactured in a facility that maintains
certifications to both ISO 9001:2008 and ISO 13485:2003. They have been designed and tested to be
compliant with the laboratory requirements of applicable regulatory agencies (only when used
with Beckman Coulter rotors). Declarations of conformity and certificates of compliance are
available at www.beckmancoulter.com.
Scope of Manual
This manual is designed to familiarize you with the Avanti J-HC centrifuge, its functions,
specifications, operation, and routine operator care and maintenance. Beckman Coulter
recommends that you read this entire manual, especially the Safety Notice and all safety-related
information, before operating the centrifuge or performing instrument maintenance.
• CHAPTER 1, Description contains system specifications and a physical and functional
description of the centrifuge, including the operating controls and indicators.
• CHAPTER 2, Operation contains centrifuge operating procedures.
• CHAPTER 3, Troubleshooting lists possible malfunctions, together with probable causes and
suggested corrective actions.
• CHAPTER 4, Care and Maintenance contains procedures for routine operator care and
maintenance, as well as a brief list of supplies and replacement parts.
• APPENDIX A, Preinstallation Requirements provides information about space and power
requirements for installing and connecting the centrifuge.
• APPENDIX B, Temperature Calibration Procedure contains a procedure to use when temperature
control within 1°C is required.
NOTE
If the centrifuge is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual is the sole responsibility of the user.
Conventions
Certain symbols are used in this manual to call out safety-related and other important information.
These international symbols may also be displayed on the centrifuge and are reproduced on the
inside back cover of this manual.

PN J3HC-IM-5AD
xiv
Introduction
Recycling Label
Typing Conventions
Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys and displays.
• Key names (for example,
START and STOP ) and display names and setup field names (for example,
TEMP°C and Rotor: ) appear in boldface type.
Recycling Label
1. the device was put on the European market after August 13, 2005 and
2. the device is not to be disposed via the municipal waste collection system of any member state
of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label please contact your dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:
Multi Compliance Label
OR
This symbol indicates compliance with:
• Recycling – Refer to the Recycling Label section in this document.
• – A “CE” mark indicates that a product has been assessed before being placed on the
market, and has been found to meet European Union safety, health, and/or environmental
protection requirements.
• 169502 – This label indicates recognition by a Nationally Recognized Testing Laboratory (NRTL)
that the instrument has met the relevant product safety standards.
A28219-AA

PN J3HC-IM-5AD xv
Introduction
Multi Compliance Label
• The RCM mark is intended for use on products that comply with Australian communications
Media Authority (ACMA) EMC Requirements.

PN J3HC-IM-5AD
xvi
Introduction
Multi Compliance Label

PN J3HC-IM-5AD 1-1
CHAPTER 1
Description
Introduction
This section describes the Avanti J-HC centrifuge components and their functions. It also describes system
safety features and centrifuge controls and displays. Refer to the applicable rotor manual for rotor
descriptions.
Centrifuge Function and Safety Features
Centrifuge Function
The Avanti J-HC high-capacity centrifuge
*
generates centrifugal forces required for applications
including pelleting of bacteria, yeast, and mammalian cells. The instrument design features a
brushless switched-reluctance drive motor,
†
FRS (friction reduction system) vacuum control
circuitry, temperature control system, rotor verification system, and a choice of acceleration and
deceleration rates.
Manual and programmed operation are available.
• In manual operation, you enter the individual run parameters before beginning each run.
• In programmed operation, you enter and save sets of run parameters. To perform a run, you
recall and start a previously saved program, enabling quick and accurate run duplication. Up to
thirty programs can be saved, each containing one or two steps.
User messages and/or audible signals are provided to alert you to conditions that may need
attention.
The centrifuge can also be operated by the SpinTrace II system for centrifuge control and data
collection. SpinTrace II software runs on a personal computer connected to up to thirty centrifuges.
Contact your Beckman Coulter representative for more information.
Safety Features
Avanti J-HC centrifuges have been designed and tested to operate safely indoors at altitudes up to
2000 m (6252 ft).
* Avanti J-HC software and firmware copyright 1999–2013 by Beckman Coulter, Inc., Brea, CA, 92821, U.S.A.
† Manufactured under license from Switched Reluctance Drives Limited, Harrogate, U.K.

PN J3HC-IM-5AD
1-2
Description
Centrifuge Chassis
Safety features include the following:
• An electromechanical door lock system prevents operator contact with spinning rotors and
prevents run initiation unless the door is shut and locked. The door locks when
START is
pressed, or when the POWER switch is turned off.
• A steel casing surrounds the rotor chamber to provide operator protection in the unlikely event
of a rotor mishap.
• An automatic rotor identification system detects which Beckman Coulter rotor is installed in
the centrifuge during acceleration and prevents the rotor from running above its maximum
rated speed.
• An imbalance detector monitors the system during operation, causing automatic shutdown if
rotor loads are severely out of balance.
Centrifuge Chassis
Housing and Door
The instrument housing is made of aluminum and molded structural foam, finished with acrylic
baking enamel. The door and structural-foam cover panels are finished with polyurethane enamel.
The control panel is covered by a protective overlay made of coated polycarbonate.
The door is opened by stepping on a foot pedal, which is located at the bottom right front of the
instrument. Initial opening is assisted by a built-in torsion bar. When the door is about three-
quarters open, a gas spring opens the door the rest of the way. The door will remain open without
support.
A small label, shown below, is located on the top left side of the chamber door above the hinge. This
label indicates a potential pinch hazard if hands or fingers are placed in the hinge area when the
door is being opened or closed. Operators and observers must take care to move their hands away
from the hinge area when the door is opened and closed.
The rubber block on the inside of the door is provided as a hanger for a rotor windshield lid while
the door is open.
In the event of a power failure, the door lock can be manually unlocked for sample recovery (see
CHAPTER 3, Troubleshooting).

PN J3HC-IM-5AD 1-3
Description
Centrifuge Chassis
1
Rotor Chamber
The rotor chamber is made of stainless steel to resist corrosion. The rotor drive hub and thermistor
are visible in the bottom of the chamber. A rubber gasket around the chamber opening ensures
sealing. (Instrument gaskets have not been qualified as bioseals for aerosol containment.)
A port in the centrifuge door provides visual access to the chamber, enabling verification of rotor
speed using a customer-supplied strobe system. See Speed Verification in CHAPTER 4 for more
information.
Friction Reduction System (FRS)
The friction reduction system (FRS) uses a mechanical rotary vane vacuum pump to reduce chamber
pressure to approximately one-quarter atmosphere (190 mm Hg). The pump turns on after the run
is started, before rotor friction reaches a high level. When the required vacuum level is reached, the
pump turns off. Vacuum in the chamber is vented automatically during rotor deceleration.
FRS is disabled when the JS-4.2 rotor is run.
Temperature Control System
The temperature control system is cooled by circulation of a non-CFC-based refrigerant. The
temperature control system is activated when the centrifuge power is on and when the door
is closed.
A thermistor in the rotor chamber continuously monitors the chamber temperature. The system
calculates the chamber temperature required to maintain the set rotor temperature, 2°C. If the
OFF deceleration setting is used, temperature is controlled to 4°C during deceleration. Although
the chamber temperature fluctuates during operation, the rotor’s large mass keeps the sample
temperature substantially constant. At the end of a run, the system continues controlling the
temperature to prevent freezing or overheating (more than 4°C over set temperature) of the sample.
Overtemp System
An overtemp (over temperature) system provides flexibility, sample protection, and safety for
the user.
• The user sets the run temperature, then sets a maximum temperature or accepts the default
maximum temperature which is 4°C over the set temperature.
• The user specifies whether the run should shut down or continue if the overtemp setting is
reached.
• The system will always shut down and end the spin using maximum brake if the temperature
goes above 50°C.

PN J3HC-IM-5AD
1-4
Description
Name Rating Plate
Drive
The drive spindle is directly driven by a brushless, high-torque, switched-reluctance motor. The
instrument’s resilient suspension minimizes disturbance of the sample during acceleration and
deceleration, and reduces damage to the drive shaft if an imbalance occurs during centrifugation.
Name Rating Plate
The name rating plate is affixed to the rear of the centrifuge. Check that the line voltage agrees with
the voltage listed on this name rating plate before connecting the centrifuge. Always mention the
model number and serial number when corresponding with Beckman Coulter regarding your
centrifuge.
Controls and Indicators
Power Switch
The power switch, located below the control panel, is a two-position rocker switch ( I , on; O , off) that
controls electrical power to the centrifuge. It is also a circuit breaker that will trip to cut off power
in the event of a power overload. The power switch must be turned on before the chamber door can
be opened.
Control Panel
The control panel (see Figure 1.1) is used to enter run parameters via touch keys and a keypad.
There are separate displays for set parameters and actual values.