Integra WELLJET 5000 User manual

WELLJET Dispenser Operating Instructions
WELLJET Dispenser Stacker
191950-V00

i/iii INTEGRA Biosciences AG
Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the devices
comply with:
Description Models
WELLJET Dispenser 5000
WELLJET Dispenser Stacker 5001
Accessory 128909
EU Directives Scope Date effective
2014/35/EU Low voltage directive (LVD) 20.04.2016
2014/30/EU Electromagnetic compatibility (EMC) 20.04.2016
2014/53/EU Radio Equipment directive (RED) 13.06.2016
2012/19/EU Waste electrical and electronic equipment
(WEEE)
14.02.2014
2011/65/EU Restriction of hazardous substances (RoHS) 03.01.2013
EU Regulations Scope Date effective
1907/2006
Registration, evaluation, authorisation and
restriction of chemicals (REACH)
01.06.2007
2019/1782
External power supply efficiency 01.04.2020
EU Standards Scope
EN 9001:2015 Quality Management
EN 61010-1:2020 Safety general laboratory equipment
EN 61326-1:2013 Electromagnetic compatibility laboratory equipment
EN 61010-2-081:2020 Safety automatic laboratory equipment
EN 301 489-1 V2.2.0 EMC radio equipment: technical requirements
EN 301 489-17 V3.2.0 EMC radio equipment: conditions for operating
EN 300 330 V2.1.1 Short range devices
EN 300 328 V2.2 Wideband data transmission systems
EN 60950-1:2013 Safety information technology equipment
EN 62368-1:2021 Safety information technology equipment

www.integra-biosciences.com ii/iii
WELLJET – Declaration of conformity
GBR Regulations Scope Date effective
S.I. 2016/1101
Electrical equipment safety 08.12.2016
S.I. 2016/1091 Electromagnetic compatibility (EMC) 08.12.2016
S.I. 2017/1206 Radio equipment (RED) 26.12.2017
S.I. 2013/3113 Waste electrical and electronic equipment
(WEEE)
01.01.2019
S.I. 2012/3032 Restriction of hazardous substances (RoHS) 02.01.2013
GBR Standards Scope
BS 61010-1:2010 Safety general laboratory equipment
BS 61010-2-081:2020 Safety automatic laboratory equipment
BS 62368-1:2020 Safety information technology equipment
BS 63000:2018 Restriction of hazardous substances (RoHS)
USA Regulations Scope
47 CFR Part 15 (FCC) Electromagnetic compatibility (EMC)
10 CFR Part 430 External power supply efficiency (CEC VI)
17 CFR Parts 240 & 249b Dodd frank “Conflict minerals”
27 CCR Parts 25102-
27001
Proposition 65: The safe drinking water and
toxic enforcement act
TSCA 40 CFR Part 751 Toxic substances control act
USA Standards Scope
UL 61010-1:2012 Safety general laboratory equipment
UL 61010-2-081:2019 Safety automatic laboratory equipment
CAN Standards Scope
CSA-C22.2
No. 61010-1
Safety general laboratory equipment
CSA C22.2
No. 61010-2-081
Safety automatic laboratory equipment

iii/iii INTEGRA Biosciences AG
WELLJET – Declaration of conformity
Zizers, August 11, 2022
CHN Regulations Scope Date effective
AQSIQ Order 5 /2001 China compulsory certification mark (CCC)
safety and EMC requirements for electrical
equipment
01.08.2003
Order 32/2016 Restriction of hazardous substances (RoHS) 01.07.2016
CHN Standards Scope
GB4943.1-2011 Information technology equipment safety
GB9254-2008 Information technology equipment radio
disturbance
GB17625.1-2012 EMC limits for harmonic current emissions
SJ/T 11364-2014 Restriction of hazardous substances (RoHS)
JPN Regulations Scope Date effective
PSE (Denan) Law
Electrical appliance and material safety law 01.01.2014
EAC Технический регламент Таможенного союза
ТР ТС 004/2011 О
безопасности низковольтного оборудования
ТР ТС 020/2011 Электромагнитная совместимость технических средств
Urs Hartmann
CEO
Daniel Bächi
Head of Corporate Quality

www.integra-biosciences.com 5
WELLJET – Operating instructions V00
Table of contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................7
1.2 Intended use.....................................................................................8
1.3 Safety notes .....................................................................................8
Chapter 2 Description of the device
2.1 Scope of delivery............................................................................10
2.2 Overview of the WELLJET .............................................................10
2.2.1 Dispenser............................................................................10
2.2.2 Interfaces ............................................................................10
2.2.3 Dispenser Stacker...............................................................11
2.2.4 Dispensing cassette............................................................11
Chapter 3 Installation
3.1 Operating environment...................................................................12
3.2 Assembling the WELLJET..............................................................12
3.2.1 Installing/removing dispensing cassettes............................12
3.2.2 Breaking off anti-collision rib ...............................................13
3.2.3 6 through 48 well dispensing ..............................................14
3.2.4 Reduce dead volume ..........................................................14
3.2.5 Tube holder.........................................................................15
3.2.6 Waste tubing .......................................................................15
3.3 WELLJET Dispenser Stacker.........................................................16
3.3.1 Inserting the towers.............................................................16
3.3.2 Inserting plates into the left tower .......................................16
3.3.3 Removing plates from tower ...............................................16
3.3.4 Plate loading adapter ..........................................................17
3.4 Settings - Adapt your WELLJET.....................................................18
3.4.1 System settings...................................................................18
3.4.2 Dispense and cleaning settings ..........................................19
Chapter 4 Operation
4.1 Turn on the device..........................................................................20
4.2 Overview of Main Menu functions ..................................................20
4.3 Running a program.........................................................................21
4.3.1 Quick start guide.................................................................21
4.3.2 Description of buttons .........................................................22

6 INTEGRA Biosciences AG
WELLJET – Operating instructions V00
4.4 Plate library ....................................................................................23
4.4.1 Plate dimensions for stacker...............................................24
4.5 Creating a new dispense program .................................................26
4.5.1 Main functions.....................................................................26
4.5.2 Advanced functions.............................................................28
4.5.3 Stacker options (model 5001 only) .....................................28
4.6 Modifying dispense programs ........................................................29
4.7 Troubleshooting/FAQ .....................................................................30
Chapter 5 Maintenance
5.1 Daily procedure ..............................................................................31
5.2 Dispensing cassettes .....................................................................31
5.2.1 Creating a cleaning program...............................................31
5.2.2 Modifying a cleaning program.............................................31
5.2.3 Flushing the tubing after use...............................................32
5.2.4 Cleaning the nozzles...........................................................32
5.2.5 Decontamination .................................................................32
5.2.6 Performance for non-aqueous solutions .............................33
5.3 WELLJET .......................................................................................34
5.3.1 Cleaning..............................................................................34
5.3.2 Decontamination .................................................................34
5.3.3 Regularly data backup ........................................................34
5.4 Servicing.........................................................................................35
5.5 Equipment disposal ........................................................................35
Chapter 6 Technical Data
6.1 Environmental conditions ...............................................................36
6.2 Specification of the WELLJET .......................................................36
6.3 Intellectual property ........................................................................37
6.4 Dispensing cassettes .....................................................................37
6.4.1 Volumes ..............................................................................37
6.4.2 Dispensing speeds..............................................................38
6.4.3 Accuracy and precision specifications ................................38
6.4.4 Life time expectancy ...........................................................39
6.4.5 Chemical compatibility ........................................................40
Chapter 7 Accessories and consumables
7.1 Accessories ....................................................................................41
7.2 Consumables .................................................................................41

WELLJET – Operating instructions V00 Introduction
www.integra-biosciences.com 7
1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the WELLJET.
1.1 Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
WARNING
This safety symbol warns against hazards that could result in injury. It also
indicates hazards for machinery, materials and the environment.
It is essential that you follow the corresponding precautions.
CAUTION
This symbol cautions against potential material damage or the loss of data in
a microprocessor controller. Follow the instructions.
NOTE
This symbol identifies important notes regarding the correct operation of the
device and labor-saving features.
The device is marked with the following symbols:
BIOHAZARD
The instrument can be potentially biohazardous due to the use of
biohazardous substances by the operator.
CRUSHING OF HANDS
Keep the plate carrier area free of any labware and keep hands away. The
hands may be squeezed, pulled in or otherwise injured by moving parts of the
instrument.
LASER CLASS 1
The sensor contains a class 1 laser which is inherently safe
under reasonably foreseeable conditions of operation.

WELLJET – Operating instructions V00 Introduction
8 INTEGRA Biosciences AG
1.2 Intended use
WELLJET a is a bulk reagent dispenser in the volume range of 0.5–9’999 µl using a
variety of tubing sets. Any use of this device in a medical or IVD setting is the responsibility
of the user.
This product may only be operated in a secure, protected network with validated,
trustworthy clients. The operator must ensure that network security measures are always
up-to-date and state-of-the-art. This product may not be directly exposed to the internet.
If WELLJET is used in a manner not specified by INTEGRA Biosciences, the protection
provided by the WELLJET may be impaired.
1.3 Safety notes
WELLJET complies to the recognized safety regulations and is safe to operate. WELLJET
can only be operated when in perfect condition and while observing these operating
instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the WELLJET must have read and understood
these operating instructions, and particularly, the safety notes, or must have been
instructed by supervisors so that safe operation of the device is guaranteed.
WARNING
Do not use the WELLJET near flammable material or in explosive areas.
Also, do not pipette highly flammable liquids such as acetone or ether.
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
CAUTION
Avoid pipetting of liquids whose vapors could attack the materials PA
(polyamide), POM (polyoxymethylene), FPM (fluor-rubber), NBR (nitrile-
rubber), CR (chloroprene), silicone. Corrosive vapors could also damage
metallic parts inside the device.
Do not open or modify WELLJET in any way. The cover must not be
removed. Repairs may only be performed by INTEGRA Biosciences or by an
authorized after-sales service member.
Parts may be replaced with original INTEGRA Biosciences parts only.
NOTE
Prolonged exposure of the WELLJET to UV-light can cause discoloration
and/or yellowing of the control unit. However, this will not affect the
performance of the device in any way.

WELLJET – Operating instructions V00 Introduction
www.integra-biosciences.com 9
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.

WELLJET – Operating instructions V00 Description of the device
10 INTEGRA Biosciences AG
2 Description of the device
2.1 Scope of delivery
• WELLJET Dispenser or WELLJET Dispenser Stacker
• Waste trough
• Waste tubing
• Rib removal tool (Dispenser) or plate measuring tool (Dispenser Stacker)
• Mains adapter
• Power cord
• Quick start guide
CAUTION
Verify the scope of delivery when unpacking the device and check for
potential transportation damage. Do not operate a device that is damaged,
instead contact your local INTEGRA representative.
2.2 Overview of the WELLJET
2.2.1 Dispenser
2.2.2 Interfaces
1Hood of pump unit, open to install dispensing
cassette
2Plate carrier with clamp mechanism
3Display
4Waste trough with drain fitting for waste tubing,
serves also as reservoir holder
5Deck
6Mounting bar for attaching the tube holder
7Lever for tightening and releasing the dispensing
cassette
8Ethernet
9USB-A ports Host: for data backup and software
updates
10 USB-B port Device: for remote control
11 Main switch
12 Socket for mains adapter
6
1
2
3
4
5
7
11
10 128 9

WELLJET – Operating instructions V00 Description of the device
www.integra-biosciences.com 11
2.2.3 Dispenser Stacker
The WELLJET Dispenser Stacker allows for microplates to be stored, dispensed to and
then stacked. Two plate towers are available with the capacity of 25 plates or 50 plates
and must be ordered separately.
2.2.4 Dispensing cassette
The dispensing cassette has a RFID tag integrated.
13 Towers with tower handles
14 Guiding rods
15 Tower doors, 2 in front of each tower
16 Tower bar with tower release lever
17 Plate carrier
18 Loading adapter, facilitates manual plate loading,
stored underneath the device
19 Plate measuring tool
20 Label
21 Tubing weight
22 Nozzles
23 Anti-collision rib
13
15
18
14
17
16
19
20
22
21
23

WELLJET – Operating instructions V00 Installation
12 INTEGRA Biosciences AG
3 Installation
3.1 Operating environment
The WELLJET has been designed for use in a laboratory. It shall be operated in a dry and
dust-free location without large temperature fluctuations or direct sunlight. Place the
WELLJET on a flat, dry, clean and vibration-proof bench. Leave at least 10 cm (3.9 in.)
space on both sides and on the back to allow adequate air circulation.
3.2 Assembling the WELLJET
Unpack the WELLJET from the packaging configuration. Two persons are required to lift
the WELLJET Dispenser Stacker. Hold the WELLJET firmly on both sides of the
instrument base. In addition, you can lift the WELLJET Dispenser Stacker on the silver
tower bars (16).
Connect the WELLJET to the power supply with the supplied mains adapter.
3.2.1 Installing/removing dispensing cassettes
Different dispensing cassettes can be used with the WELLJET, see 7.2:
The life time depends on the volume dispensed, see 6.4.2.
EasySnap™ dispensing cassette Can be used for
8 channel, small bore 96, 384 or 1536 well plates
8 channel, large bore 96 or 384 well plates
16 channel, small bore 384 or 1536 well plates
1) To insert the cassette, open the hood and turn
the lever to the right ( ).
2) Hold the cassette with one hand, the tubings
are showing backwards.
3) Insert the two pins (3) into the slots of the
holder.
4) Move the front part of the cassette down until
the two pins (4) audibly click into position.
5) Turn the lever backwards ( ) to tighten the
cassette. Alternatively, press down the rear
part of the cassette (3) until it clicks into
position ( ).
6) Place the tubings in the opening of the holder
and close the hood.

WELLJET – Operating instructions V00 Installation
www.integra-biosciences.com 13
NOTE
The tutorial for cassette loading/removal can be switched on/off under
System settings - Display.
3.2.2 Breaking off anti-collision rib
Removing the rib allows dispensing inside the wells, which can improve precision when
dispensing into a 384 or 1536 well plate and can also prevent the buildup of liquid on the
nozzles when dispensing viscous reagents.
1) To remove the cassette, open the hood and
turn the lever to the right as far as possible
().
2) Pull the cassette straight out.
Anti-collision rib of dispensing cassette, protects
the nozzles from crashes
Guide the slit of the delivered rib removal tool or
of the scale bar (part of plate measuring tool) and
break off the anti-collision rib carefully at the 3
predetermined breaking points (see arrows).
Ensure the dispensing nozzles are not damaged.
Rip removal tool
Plate measuring tool with scale bar (a)
a
a

WELLJET – Operating instructions V00 Installation
14 INTEGRA Biosciences AG
3.2.3 6 through 48 well dispensing
For use of an 8 channel cassette to dispense 2, 3, 4, or 6 rows on 6, 12, 24, or 48 well
plates respectively, the tubings must be adapted.
In the dispensing program select the correct 8 channel tubing set and the number of wells.
3.2.4 Reduce dead volume
To reduce the dead volume of the dispensing cassette, you can shorten the source
tubings. Hang the tubing into a source vessel and determine the length to be cut. Note the
length! The original length of source tubing is 50 cm.
Subtract the cut length from the original length. For example, if you cut off 10 cm, the new
length is 50 cm - 10 cm = 40 cm. Enter the new length under Dispense/Cleaning Settings
- Tubing length to enable the calculation of the new dead volume.
Attach an 8 channel cassette.
Follow the instructional diagram below to
disconnect the appropriate tubing from the
source tubing weight (21). Tubing one is the
leftmost in the cassette.
Connected nozzles
12345678
6 well plate (2x3)    
12 well plate (3x4) 
24 well plate (4x6) 
48 well plate (6x8) 
Remove the source tubing weight.
Lay out the tubings on a lint free, flat surface. Use
a ruler to measure the length that should be cut.
Using a razor blade or a pair of shaping scissors,
cut pieces of equal length from all the tubing
ends, one after the other and as straight as
possible.

WELLJET – Operating instructions V00 Installation
www.integra-biosciences.com 15
3.2.5 Tube holder
3.2.6 Waste tubing
Slide the tube holder over the mounting bar (6).
Connect the waste tubing to the drain fitting of the
waste trough.
Alternatively insert a 25 ml reservoir in the waste
trough, see “7.2 Consumables” on page 41.

WELLJET – Operating instructions V00 Installation
16 INTEGRA Biosciences AG
3.3 WELLJET Dispenser Stacker
3.3.1 Inserting the towers
Hold a tower on the guiding rods and put it on the stacker. Slide the tower to the back until
it audible clicks into position. Repeat with the second tower.
3.3.2 Inserting plates into the left tower
3.3.3 Removing plates from tower
Open the tower doors (15) and slide a stack of
empty plates to the back of the left tower.
Close the tower doors.
CAUTION
The right tower needs to be empty
when starting a run. Otherwise the
plates will fall down.
The dispensed plates are stacked in the right
tower.
While pressing the tower release lever (16) hold
a guiding rod and slide the tower a little bit to the
front.

WELLJET – Operating instructions V00 Installation
www.integra-biosciences.com 17
3.3.4 Plate loading adapter
Hold the tower with one hand on the handle and
the other hand on the frame at the bottom of the
tower.
Lift and remove the tower.
Pull out the loading adapter (18) from underneath
the device.
Put the adapter on the plate carrier. It serves as a
frame for the plate to facilitate plate loading.

WELLJET – Operating instructions V00 Installation
18 INTEGRA Biosciences AG
3.4 Settings - Adapt your WELLJET
Press the -icon in the upper right corner of the VIAFILL Main menu to access the
Settings. Press to save your settings.
3.4.1 System settings
Software update
Download the latest software from INTEGRA’s website to a newly formatted USB drive of
the standard FAT32 format. Unzip the software on the USB with the Windows unzip
program installed. Move both software files (.bin and .upd) to the top level of the USB
drive. On your WELLJET select System settings - Software update, connect the USB drive
and press Reboot. If the USB drive is not detected by the device, please contact
INTEGRA.
System Settings Description
Language Choose the language in which all screens are displayed.
Display Sets brightness of the display (Low, Medium, High). Allows to
switch off the display of tutorials (white bar).
Password Allows to change or remove a device password of 4-12 digits.
Enter the default password “WELLJET”. The password is used
to protect programs or plate layouts.
Reset Device Resets the device with all settings including the password and
deletes all programs and the labware library.
Date and Time Sets date and time.
Device Information Displays the serial number and software version. Allows to enter
an owner name.
Backup Backups the settings, labware library and dispense and cleaning
programs to a USB drive inserted in one of the two USB ports ().
The data can be imported to another device.
Connectivity Allows remote control using API commands via a PC.
Sounds Allows beep tones to be turned on or off. Move the bar beside an
option to the right to activate the sound, e.g. at program end, and
set the volume (Low, Medium, High).
Software update Allows to load the latest software version from INTEGRA’s
website via a USB drive.
Import Imports the device settings, labware library and dispense and
cleaning programs from a USB drive. It also removes the device
password.
Maintenance Shows the date of the last maintenance, allows to set a service
reminder and to save the device log file to a USB drive.

WELLJET – Operating instructions V00 Installation
www.integra-biosciences.com 19
3.4.2 Dispense and cleaning settings
Dispense/Cleaning
Settings Description
Prime Defines the prime speed and the default volume in number of
cassette volumes.
Clog Prevention Activates an automatic pump backward rotation in direction of
the source container after a defined idle time in order to prevent
clog building in the dispensing nozzles.
Program Categories Allows to define, edit and delete program categories.
Recover Defines the recover speed and the default volume in cassette
volumes.
Cassette Information Shows information on the RFID tag1of the inserted cassette,
such as lot number, number of channels, cassette validation
date, life time, volume counter.
1. The RFID tag allows tracking of cassette usage by incrementing rotations of
the pump. The number of rotations is compared to the specific lifetime value of
each cassette.
Cleaning Reagents Allows to define, edit and delete cleaning reagents.
Shake Sets the shake speed (Fast, Medium, Slow) and shake time.
Tubing Length Allows to save the tubing length of the cassette, e.g. if you had
to shorten the tubing length to reduce the dead volume. Insert
the cassette, enter the new tubing length and save it to the RFID
tag of the cassette. The length is considered for priming and
washing volume when using cassette volumes.

WELLJET – Operating instructions V00 Operation
20 INTEGRA Biosciences AG
4 Operation
4.1 Turn on the device
Turn on the WELLJET by pressing the main switch (11).
CAUTION
Remove hands from WELLJET during initialization.
Press OK to start initialization. After initialization the Main Menu is displayed.
4.2 Overview of Main Menu functions
The Main menu shows the recently used dispense programs. On the right side additional
options are available:
To return to the Main menu, press home ( ).
Programs Description
Library Opens the plate library where dimensions of the used microplates
or tube racks can be defined.
Settings The system, dispense and cleaning settings provide options to
adapt the WELLJET to appropriate applications.
New:
Dispense
Creates a new dispense program. On the Main Functions screen
all commonly used parameters can be defined. The Advanced
menu offers additional settings.
Dispense
Programs
Opens a list of saved dispense programs in alphabetical order.
Plate, category, author, protections and favorite filters can be set.
New:
Cleaning
Creates a new cleaning program to wash the cassette.
Cleaning
Programs
Opens the list of saved cleaning programs.